Publications by authors named "Hicham Naji Amrani"

10 Publications

  • Page 1 of 1

Prevalence and risk factors for latent tuberculosis infection among healthcare workers in Morocco.

PLoS One 2019 15;14(8):e0221081. Epub 2019 Aug 15.

Genetics Unit, Military Hospital Mohamed V, Rabat, Morocco.

Increased prevalence of latent tuberculosis infection (LTBI) has been observed among high-risk populations such as healthcare workers (HCWs). The results may depend on the method of LTBI assessment, interferon-gamma release assay (IGRA) and/or tuberculin skin test (TST). Here, we investigated the prevalence and risk factors for LTBI assessed by both IGRAs and TST in HCWs living in Morocco, a country with intermediate tuberculosis (TB) endemicity and high BCG vaccination coverage. HCWs were recruited in two Moroccan hospitals, Rabat and Meknes. All the participants underwent testing for LTBI by both IGRA (QuantiFERON-TB Gold In-Tube, QFT-GIT) and TST. Different combinations of IGRA and TST results defined the LTBI status. Risk factors associated with LTBI were investigated using a mixed-effect logistic regression model. The prevalence of LTBI among 631 HCWs (age range 18-60 years) varied from 40.7% (95%CI 36.9-44.5%) with QFT-GIT to 52% (95%CI 48.2-56.0%) with TST using a 10 mm cut-off. The highest agreement between QFT-GIT and TST (κ = 0.50; 95%CI 0.43-0.56) was observed with the 10 mm cut-off for a positive TST. For a definition of LTBI status using a double positive result for both QFT-GIT and TST, significant associations were found with the following risk factors: being male (OR = 2.21; 95%CI 1.40-3.49; p = 0.0007), belonging to age groups 35-44 years (OR = 2.43; 95%CI 1.45-4.06; p = 0.0007) and even more 45-60 years (OR = 4.81; 95%CI 2.72-8.52; p = 7.10-8), having a family history of TB (OR = 6.62; 95%CI 2.59-16.94; p = 8.10-5), and working at a pulmonology unit (OR = 3.64; 95%CI 1.44-9.23; p = 0.006). Smoking was associated with LTBI status when defined by a positive QFT-GIT result (OR = 1.89; 95%CI 1.12-3.21; p = 0.02). A high prevalence of LTBI was observed among HCWs in two Moroccan hospitals. Male gender, increased age, family history of TB, and working at a pulmonology unit were consistent risk factors associated with LTBI.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0221081PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695119PMC
March 2020

Tuberculosis and impaired IL-23-dependent IFN-γ immunity in humans homozygous for a common missense variant.

Sci Immunol 2018 12;3(30)

St. Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, Rockefeller University, New York, NY, USA.

Inherited IL-12Rβ1 and TYK2 deficiencies impair both IL-12- and IL-23-dependent IFN-γ immunity and are rare monogenic causes of tuberculosis, each found in less than 1/600,000 individuals. We show that homozygosity for the common P1104A allele, which is found in about 1/600 Europeans and between 1/1000 and 1/10,000 individuals in regions other than East Asia, is more frequent in a cohort of patients with tuberculosis from endemic areas than in ethnicity-adjusted controls ( = 8.37 × 10; odds ratio, 89.31; 95% CI, 14.7 to 1725). Moreover, the frequency of P1104A in Europeans has decreased, from about 9% to 4.2%, over the past 4000 years, consistent with purging of this variant by endemic tuberculosis. Surprisingly, we also show that TYK2 P1104A impairs cellular responses to IL-23, but not to IFN-α, IL-10, or even IL-12, which, like IL-23, induces IFN-γ via activation of TYK2 and JAK2. Moreover, TYK2 P1104A is properly docked on cytokine receptors and can be phosphorylated by the proximal JAK, but lacks catalytic activity. Last, we show that the catalytic activity of TYK2 is essential for IL-23, but not IL-12, responses in cells expressing wild-type JAK2. In contrast, the catalytic activity of JAK2 is redundant for both IL-12 and IL-23 responses, because the catalytically inactive P1057A JAK2, which is also docked and phosphorylated, rescues signaling in cells expressing wild-type TYK2. In conclusion, homozygosity for the catalytically inactive P1104A missense variant of selectively disrupts the induction of IFN-γ by IL-23 and is a common monogenic etiology of tuberculosis.
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http://dx.doi.org/10.1126/sciimmunol.aau8714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341984PMC
December 2018

[Pulmonary nocardiosis in immunocompetent patients: about 2 cases].

Pan Afr Med J 2017 29;27:149. Epub 2017 Jun 29.

Service de Pneumo-Phtisiologie, Hôpital Moulay Youssef, Rabat, Maroc.

Nocardiosis is a rare but severe infection caused by bacteria of the genus nocardia, which belong to the order actinomycetales. If they can affect immunocompetent adult, nocardioses are pathologies affecting the individuals with weakened immune system. Pulmonary involvement is the most common manifestation, its correct management is based on diagnosis, which is often delayed due to non-specific symptoms and inconclusive specimens. We here report two cases of nocardiosis in immunocompetent patients. The first case concerns a 24-year old man with a history of smoking and alcoholism, hospitalized for chest pain and hemoptysis of low abundance evolving for two months, associated with the occurrence of dorsal subcutaneous fistulized abscess. Radiological assessment showed right mediastino-pulmonary tissue mass associated with adjacent costal lysis and dissemination in rights paravertebral tissues. Bacteriological sampling remained negative motivating ultrasound-guided biopsy of the lesion, which confirmed the diagnosis of nocardia infection. The second case concerns a 22-year old man with a history of pleural tuberculosis treated 8 years ago and of relapse of tuberculosis in 2011 (mediastinal abscess). He was admitted to hospital due to suspicion of relapse of tuberculosis based on chronic cough with alteration of general state and hepatosplenomegaly. Chest CT scan showed alveolar condensations with pleurisy. During his hospitalization, purulent subcutaneous swellings occurred. Bacteriological analysis of the pus confirmed the diagnosis of nocardiosis. Nocardia strains were resistant to all antibiotics except for colistin and bactrim. This study aims to highlight the clinical and radiological aspects of pulmonary nocardiosis, focusing on diagnostic and therapeutic difficulties especially in a country with a high prevalence of tuberculosis and a very low incidence of nocardiosis.
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http://dx.doi.org/10.11604/pamj.2017.27.149.12862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567944PMC
September 2017

Chilaiditi syndrome.

Pan Afr Med J 2017;26:129. Epub 2017 Mar 7.

Department of Pneumology, Moulay Ismail Military Hospital, Meknes, Morocco.

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http://dx.doi.org/10.11604/pamj.2017.26.129.11557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429429PMC
March 2017

[Left pulmonary artery agenesis].

Pan Afr Med J 2016;25:181. Epub 2016 Nov 21.

Service de Pneumologie, Hôpital Militaire d'Instruction Mohammed V, Rabat, Maroc.

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http://dx.doi.org/10.11604/pamj.2016.25.181.10388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326040PMC
April 2018

[Severe pulmonary involvement in hypocomplementemic urticarial vasculitis (HUV)].

Pan Afr Med J 2016 28;24:285. Epub 2016 Jul 28.

Service de Pneumologie, Hôpital Militaire Mohamed V, Rabat, Maroc.

Pulmonary involvement in hypocomplementemic urticarial vasculitis (HUV) or Mac Duffie syndrome is extremely rare with a poor prognosis. We report the case of a 55-year-old female patient treated for HUV over a period of 20 years. The diagnosis was confirmed on the basis of urticarial lesions, ocular inflammation, positive C1q-p test by immunodiffusion, with low rate of C1q. The patient was treated with cycles of cyclophosphamide, corticoids and rituximab as she developed class III dyspnea (NYHA classification ). The clinico-radiological and functional assessment showed thoracic distension and severe obstructive pulmonary disease which found no significant improvement with systemic treatment Aerosol therapy was started and the patient had a marked clinical improvement. Pulmonary involvement in Mac Duffie hypocomplementemic urticarial vasculitis worsens the patient short-term vital prognosis. The knowledge of the different types of pulmonary involvement opens new therapeutic prospects.
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http://dx.doi.org/10.11604/pamj.2016.24.285.8168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267844PMC
March 2017

[Intramedullary metastasis of lung adenocarcinoma: about a case].

Pan Afr Med J 2015 14;20:37. Epub 2015 Jan 14.

Service de Pneumo-Phtisiologie, Hôpital Militaire d'Instruction Mohammed V, CHU Rabat, Maroc.

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http://dx.doi.org/10.11604/pamj.2015.20.37.5500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4441140PMC
March 2016

[Do smokers know the harmful effects of tobacco?].

Pan Afr Med J 2014 3;19:127. Epub 2014 Oct 3.

Service de Pneumologie, Hôpital Militaire d'Instruction Mohammed V, Rabat, Maroc.

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http://dx.doi.org/10.11604/pamj.2014.19.127.5163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341264PMC
October 2015