Dr. Hesham N. Mustafa, MD - King Abdulaziz University - Associate Professor

Dr. Hesham N. Mustafa

MD

King Abdulaziz University

Associate Professor

Jeddah, Western Region | Saudi Arabia

Main Specialties: Biology

Additional Specialties: Cell Biology - Electron Microscopy

ORCID logohttps://orcid.org/0000-0003-1188-2187


Top Author

Dr. Hesham N. Mustafa, MD - King Abdulaziz University - Associate Professor

Dr. Hesham N. Mustafa

MD

Introduction

Dr. Hesham N. Mustafa has received his MD in Basic Medical Sciences (Anatomy) from Ain Shams University, Cairo, Egypt during the period of 2005-2009. Currently, he is working as Associate Professor in Basic Medical Sciences (Anatomy) department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. He has successfully completed his Administrative responsibilities as Coordinator of the Nutrition and Metabolism Module & Coordinator of the E-Library Services in the Health colleges Library & Coordinator of the Academic Advising Unit, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. He is serving as an editorial member of several reputed journals like International Journal of Animal Biology (IJAB) & Journal of Nanoscience and Nanoengineering & Biological and Chemical Research Journal & Clinical Medicine Journal & Peertechz Journal of Cytology and Pathology & Journal of Diabetes Mellitus and Metabolic Syndrome (JDMMS). He has authored 11 research articles in ISI Journals. He is a member of European Society for the Study of Peripheral Nerve Repair and Regeneration (ESPNR) & the Institute of Research Engineers and Doctors (IRED) & Egyptian Society of Basic Medical Sciences & Arab Society for Medical Research & Egyptian Anatomical Society & Student Support Unit at the Faculty of Medicine, King Abdulaziz University & Curriculum Development Committee of First phase for the academic years in the Faculty of Medicine, King Abdulaziz University. He has honoured the Award of Excellence of Scientific Publication for the staff members 2012, from Deanship of Scientific Research, King Abdulaziz University.

Primary Affiliation: King Abdulaziz University - Jeddah, Western Region , Saudi Arabia

Specialties:

Additional Specialties:

Research Interests:


View Dr. Hesham N. Mustafa’s Resume / CV

Education

Jan 2005 - Jan 2009
Ain Shams University Faculty of Medicine
M.D. Degree in Basic Medical Sciences “ANATOMY”
Anatomy and Embryology
Jan 2001 - Jan 2005
Ain Shams University Faculty of Medicine
Master Degree in Basic Medical Sciences “ANATOMY"
Anatomy and Embryology
Jan 1999 - Jan 2001
Cairo University KasrAlainy School of Medicine
First Part, Master degree in Tropical Medicine
Tropical Medicine
Jan 1988 - Jan 1994
Cairo University KasrAlainy School of Medicine
M.B.B.Ch., Medicine and Surgery
M.B.B.Ch

Experience

Jan 2010 - Jul 2016
King Abdulaziz University Faculty of Medicine
Associate Professor
Anatomy
Aug 2010
King Abdulaziz University, Faculty of Medicine
Assistant Professor
Anatomy
Jan 2006 - Aug 2006
King Abdulaziz University Faculty of Medicine
Lecturer
Anatomy
Jan 2005 - Aug 2005
Misr University for Science and Technology College of Medicine
Lecturer
Anatomy
Jan 1998 - Oct 1998
Misr University for Science and Technology College of Medicine
Demonstrator
Anatomy

Publications

22Publications

508Reads

33Profile Views

120PubMed Central Citations

Liver ischemia/reperfusion injury, a setting in which the functional mass is reduced and the role of PDE5 inhibitor

Eur J Anat, 23 (5): 325-332 (2019)

European Journal of Anatomy

Liver ischemia reperfusion is induced during sur-gical procedures like liver transplantation and re-section. Multiple mechanisms have been postulat-ed to liver damage following liver ischemia reperfu-sion injury, such as oxidative stress and inflamma-tory reactions. The present study declares the pos-sible mechanism of tadalafil, toward modulating the inflammatory response. Forty-eight rats were divided into 4 groups as follows; Sham group sub-jected to midline laparotomy only. Tadalafil group administered Tadalafil 10 mg/kg intraperitoneal 45 min before sham operation. I/R (Ischemia-reperfusion) group, rats undergo 60 min of hepatic ischemia followed by 60 min of reperfusion. Tada-lafil + I/R group rats undergo a similar pattern of I/R after the treatment with Tadalafil 10 mg/kg, 45 min before ischemia. At the end of the reperfusion, the blood samples were collected for estimation of biochemical markers including liver enzymes using colorimetric assay method and serum: TNF-α (tumor necrosis factor-α), IL-6 (interleukin 6) le-vels, ICAM- 1 (Intercellular Adhesion Molecule-1) were measured. Tissues were evaluated by semi-quantitative and morphometrical approaches. Ta-dalafil succeeded in restoring normal levels of liverenzymes and ameliorating the oxidative stress as evidenced by decreasing MDA and restoring redu-ced glutathione levels in liver tissue homogenate. Also, Tadalafil exhibits anti-inflammatory effects, as it significantly decreased the levels of TNF-α, IL6 and ICAM-1. The findings are supported by BCL-2, TNF-α immunomarkers. It is concluded that modulation of the inflammatory response might be one of the mechanisms of Tadalafil-mediated he-patoprotection, so it is recommended as an adju-vant therapy in liver surgery.

Keywords: Ischemia/reperfusion injury – Oxidati-ve stress – Apoptosis – TNF-α – BCL-2

View Article
September 2019

Impact Factor 0.380

Protective effect of garlic extract against maternal and foetal cerebellar damage induced by lead administration during pregnancy in rats.

Folia Morphol (Warsz) 2018;77(1):1-15. Epub 2017 Jul 13.

King Abdulaziz University, Department of Anatomy, Faculty of Medicine, King Abdulaziz University, P O Box 80205, 21589 Jeddah, Saudi Arabia.

Background: In spite of its industrial usefulness and varied daily uses, lead (Pb) pollution is a widespread ecological problem that faces the humans in the 21th century. Pb was found to produces a wide range of toxic effects including neurotoxicity especially to the developing and young offspring. Recently, the utilisation of herbal plants has received a significant attention where there has been rising awareness in their therapeutic use; among these is the garlic. In light of the above, the current study is designed experimentally in female pregnant rats in order to investigate the beneficial role of garlic extract in the protection from the maternal and foetal cerebellar damage produced by administration of different doses of Pb during pregnancy.

Materials And Methods: Positively pregnant female rats were divided into five groups; one control group, two Pb-treated groups (exposed to 160 and 320 mg/kg b.w. of Pb, respectively) and two groups treated with both Pb and garlic (exposed to Pb as previous groups together with 250 mg/kg b.w./day of garlic extract). Treatments started from day 1 to day 20 of pregnancy, where the mother rats of different experimental groups were sacrificed to obtain the foetuses. Pb level in the maternal and foetal blood and cerebellum was estimated by spectrophotometry. Specimens of the cerebellum of different mother and foetal groups were processed to histological and immunohistochemical staining for microscopic examination.

Results: The results showed that administration of Pb to pregnant rats resulted in a dose-dependent toxicity for both mothers and foetuses in the form of decrease in maternal weight gain, placental and foetal weights, brain weight and diminished foetal growth parameters, which were prominent in rat's group treated with larger dose of Pb. In Pb-treated rats, Pb level in blood and cerebellum was high when compared with the control group. The histopathological examination of the cerebellum of treated dams and foetuses showed marked alterations mainly in the form of Purkinje cell degeneration and lack of development of foetal cerebellum. Co-treatment of garlic extract along with Pb resulted in a significant decrease in Pb levels as compared with those treated with Pb alone with improvement of the histopathological changes.

Conclusions: This study was useful in evaluating the hazardous effects of uncontrolled use of Pb in general and in assessing the developmental and neurotoxicity of foetuses due to exposure during pregnancy in particular. Co-administration of garlic has beneficial effects in amelioration of Pb-induced neurotoxicity and reversing the histopathological changes of the cerebellum of mother rats and foetuses. (Folia Morphol 2018; 77, 1: 1-15).

View Article

Download full-text PDF

Source
http://dx.doi.org/10.5603/FM.a2017.0063DOI Listing
August 2019
14 Reads
2 Citations
0.524 Impact Factor

Neuro-amelioration of cinnamaldehyde in aluminum-induced Alzheimer's disease rat model.

Authors:
Hesham N Mustafa

J Histotechnol 2019 Aug 28:1-10. Epub 2019 Aug 28.

Anatomy Department, Faculty of Medicine, King Abdulaziz University , Jeddah , Saudi Arabia.

Aluminum (Al) is a neurotoxic substance which has played an important role in the etiology, pathogenesis, and development of amyloid-? (A?) plaques. This study was carried out to evaluate the neuroprotective effect of aqueous cinnamon extract against aluminum chloride (AlCl)-induced Alzheimer's disease. Forty adult male albino rats, randomly divided into four equal groups. Control group; ACE200 group administered aqueous cinnamon extract (ACE) orally; AlCl group received daily intraperitoneal (i.p.) injection of AlCl for 60 days to induce neurotoxicity and AlCl + ACE200 group received a combination of AlCl and ACE in the same dose and route as previous groups. Aluminum administration significantly enhanced the memory impairment and the A? formation in the rat model. The cerebellum exhibited a significant reduced number of Purkinje cells, marked decrease in the density of dendritic arborization and prominent perineuronal spaces in the molecular layer. There was loss of dendritic spines, neurofibrillary degeneration, and appearance of neuritic plaques. Concomitant administration of AlCl and ACE displayed an observable protection against these changes with progressive improvement in memory and intellectual performance. In conclusion, ACE may play a protective role against formation of amyloid-? plaques in cerebellum.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01478885.2019.1652994DOI Listing
August 2019
0.306 Impact Factor

Analytical Study of Clinicopathological Data of Saudi Patients with Osteoarthritis Subjected to Total Knee Arthroplasty

figshare

SUMMARY: Knee osteoarthritis (OA) is a common disabling disease. Epidemiological studies have revealed various risk
factors for OA, including sex, aging, obesity, occupational illnesses, and chronic diseases. Here we evaluate the clinical, pathological,
and radiological findings of knee OA in a subset of Saudi patients who were subjected to total knee replacement (TKA). The study
population included 30 Saudi patients with knee OA who were operated by TKA (from June 2014 to December 2015) in the Department
of Orthopedics, Faculty of Medicine, King Abdulaziz University, Saudi Arabia. Patient’s clinical and radiological data were collected
from the hospital files. Pathological examination of the excised superior articular surface of tibia and femoral condyles were done.
Pearson Chi-squared analysis was used to test for differences between the variables in associated risk factors. There were more women
than men. Sixty per cent of patients were older than 60 years [mean age, 59.2 (females) and 61.7 (men) years-old]. All patients exceeded
obesity class 1, with females being more obese than males. Pathological examination of the superior articular surface of tibia and femoral
condyles showed high score lesions, which was more apparent in females than in males. Radiological findings showed that most lesions
were high grade. The findings of this study will help to understand the pathogenesis of OA and improve treatment decision making
relevant to TKA in knee OA in Saudi Arabia and elsewhere.

View Article
August 2019

Impact Factor 0.470

A Study on the Toxic Effect of Different Doses of Diclofenac Sodium on the Development of the Kidney in the Postnatal Period

figshare

SUMMARY: The toxic effects of different doses of diclofenac sodium (DS) on the kidney on the postnatal period (0-7 days) by
morphometrical and immunohistochemical methods were investigated. For this purpose, 15 female adult wistar albino rats were used and
divided into 5 main groups. Group Ia served as normal control, physiologic group Ib received normal saline, group II received low dose (3.9
mg/kg), group III received medium dose (9 mg/kg) and group IV received high dose (18 mg/kg). Male offspring’s from 0-7 days after birth
were used in this study. On the 8th day of postnatal life, all animals were anesthetized. Then, the kidney samples were analyzed. Haematoxylin
and eosin staining showed degeneration and necrosis, apparent atrophy of the glomeruli, mononuclear cell infiltration, congested vessels,
increased fibrous tissue and distortion of the proximal convoluted tubules with interruption of the brush margin of the DS treated group.
Increased level of Caspase-3 and upregulation of TNF-α with different doses of DS. In light of our findings, DS may lead to adverse effects
that are dose-dependent in the prenatal subjected kidney to this drug.

View Article
July 2019

Impact Factor 0.470

Does allicin combined with vitamin B-complex have superior potentials than alpha-tocopherol alone in ameliorating lead acetate-induced Purkinje cell alterations in rats? An immunohistochemical and ultrastructural study.

Folia Morphol (Warsz) 2016;75(1):76-86. Epub 2015 Sep 14.

Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.

Background: The current article aims to explore the protective potentials of ?-tocopherol alone and the combination of allicin and vitamin B-complex against lead-acetate neurotoxicity on the cerebellar cortex.

Materials And Methods: Forty rats were divided into four groups (n = 10). Group 1 was the control group; Group 2 received 10 mg/kg body weight (BW) of lead acetate; Group 3 was exposed to 10 mg/kg BW of lead acetate plus a combination of allicin (100 mg/kg BW) and vitamin B-complex (40 mg/kg BW); Group 4 was administered lead acetate (10 mg/kg BW) and ?-tocopherol (100 mg/kg BW). The animals received the treatment for 60 days by oral gavage. All the groups were studied ultrastructurally and immunohistochemically with glial fibrillary acidic protein (GFAP).

Results: The affected groups revealed shrunken and degenerated Purkinje cells with irregular nuclei. The cytoplasm comprised several lysosomes, unhealthy mitochondria, and dilated Golgi saccules. The myelinated nerve fibres demonstrated breaking of the myelin sheaths, apparent vacuoles, and broad axonal spaces. Immunohistochemically, there was a tremendous surge in GFAP-positive astrocytes in the lead acetate-treated group. These histological and ultrastructural variations were ameliorated by the administration of a-tocopherol and the combination of allicin and vitamin B complex. Moreover, an apparent decrease in the number of GFAP-positive astrocytes was obvious in the protected groups.

Conclusions: Although both a-tocopherol and the combination of allicin and vitamin B-complex can be used as possible adjuvant therapies to ameliorate nervous system ailments attributable to lead acetate, ?-tocopherol showed more protective potential.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.5603/FM.a2015.0076DOI Listing
October 2018
36 Reads
13 Citations
0.524 Impact Factor

Immunohistochemical Study of the Ameliorative Effect of Vitamin E on Liver Regeneration after Different Periods of Partial Hepatectomy

Halawani M. M, Aziz G. S. A, Amin H. A, Mustafa H. N, Elhaggagy A. A. Immunohistochemical Study of the Ameliorative Effect of Vitamin E on Liver Regeneration after Different Periods of Partial Hepatectomy. Biomed Pharmacol J 2018;11(2).DOI : http://dx.doi.org/10.13005/bpj/1419

Biomedical and Pharmacology Journal

The liver is almost unique in its capacity for regeneration after hepatectomy but the exact mechanisms are not yet fully clarified. Antioxidants have been shown to promote liver regeneration after major hepatectomy. The present study evaluated the ameliorative effect of vitamin E administration on the liver regeneration after different periods of partial hepatectomy (PH) in rats. Fifty-six adult male albino rats were divided into three groups: Control sham operated group; partially hepatectomized group which were divided into three subgroups sacrificed at 1day, 3 days and 7days after the operation respectively; Partially Hepatectomized group with vitamin E pretreatment before PH where the rats were given a daily oral dose of vitamin E until the time of sacrifice of the rats. Immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and labeling index were demonstrated. After PH, the PCNA positive hepatocytes and the PCNA labeling indices were significantly high after the 1st day and then much decreased after the 3rd day, to be followed by a slight increase at the 7th day. Vitamin E pretreatment in PH rats resulted in a decrease in PCNA positive cells and its labeling indices in the 1st day with a gradual increase in the 3rd and 7th days. Vitamin E has an inhibitory effect in the first 24 hours on liver regeneration followed by stimulatory effect at the third and seventh days after PH. These data indicated that vitamin E pretreatment has an important role in regulation and enhancement of liver regeneration after PH. Keywords: Immunohistochemistry; Labeling Index; Vitamin E; Partial Hepatectomy; PCNA

http://biomedpharmajournal.org/vol11no2/immunohistochemical-study-of-the-ameliorative-effect-of-vitamin-e-on-liver-regeneration-after-different-periods-of-partial-hepatectomy/

View Article
June 2018

Impact Factor 0.200

3 Reads

Beneficial Effects of Curcumin Inmaternal and Fetal Oxidativestress and Brain Damage Induced by Gestational Lead Administration

Saleh H. A, El-Aziz G. S. A, Mustafa H. N, El-Fark M, Tashkandi J. M, Alzahrani A. H, Mal A, AboRass M, Deifalla A. H. Beneficial Effects of Curcumin Inmaternal and Fetal Oxidativestress and Brain Damage Induced by Gestational Lead Administration. Biomed Pharmacol J 2018;11(2).DOI : http://dx.doi.or

Biomedical and Pharmacology Journal

This study was planned to explore the protective role of curcumin (Cur) against maternal and fetal oxidative stress and cerebral damage induced by lead (Pb) during pregnancy. Positively pregnant female rats were divided into seven groups: control group, Cur group (300 mg/kg of Cur/b.wt.), DMSO group (50% DMSO), two Pb-treated groups (exposed to 160 and 320 mg/kg b.wt./day of Pb acetate, respectively), and two groups treated with both Pb and Cur (exposed to Pb as previous groups together with 300 mg/kg b.wt./day of Cur). Treatments through oral gavage once a day started from gestation day 1 (GD1) till day 20 (GD20), where the mother rats of different experimental groups were sacrificed to obtain the fetuses. Different chemical parameters were assessed. Brain specimens of mother and fetal groups were processed with examination. The results displayed that Pb administration to pregnant rats resulted in a dose-dependent toxicity for both mothers and fetuses. Also, there was a significant rise in lipid peroxidation and decreased antioxidant enzyme activities in the brains of the different Pb-treated groups. The histological examination of the brain of treated dams and fetuses showed marked alterations. Co-treatment of Cur along with Pb caused a significant decrease in Pb levels as compared with those treated with Pb alone, improving the oxidative condition with amelioration of the brain’s histopathological changes. Co-administration of Cur could have ameliorative effect against Pb-induced neurotoxicity through the reduction of oxidative stress and reversal of histopathological changes. Keywords: Lead; Oxidative Stress; Brain; curcumin; Fetal toxicity

http://biomedpharmajournal.org/vol11no2/beneficial-effects-of-curcumin-inmaternal-and-fetal-oxidativestress-and-brain-damage-induced-by-gestational-lead-administration/

View Article
June 2018

Impact Factor 0.200

3 Reads

The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in a Streptozotocin-Induced Diabetic Model

Hegazy G. A, Mustafa H. N, Atahi R. M, Yousef J. M. The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in A Streptozotocin-Induced Diabetic Model. Biomed Pharmacol J 2018;11(1), 285-303. Available from: http://biomedpharmajournal.org/?p=

Biomedical & Pharmacology Journal

Renal ischemia/reperfusion injury (IRI) represents the main reason for acute kidney injury (AKI). Dexmedetomidine (Dex) and Benincasa cerifera (BC) have wide benefits due to their anti-inflammatory and antioxidant properties. This study aims to illustrate the protective effects of BC and Dex on renal IRI in a diabetic model. Sixty adult male albino rats (Wistar strain), weighing 250–300 g, were included in the study. The rats were divided into four groups, as follows: sham group: non-diabetic; diabetes mellitus (DM) + IRI group: streptozotocin (STZ)-induced diabetic rats exposed to renal IRI on day 30 after diagnosis of diabetes; DM + IRI + BC group: STZ-induced diabetic rats treated with BC (500 mg/kg) for 30 days after diagnosis of diabetes, then exposed to renal IRI; and DM + IRI + Dex group: STZ-induced diabetic rats treated with Dex (100 µg/kg intraperitoneally) 5 min before induction of ischemia on day 30 after diagnosis of diabetes, then exposed to renal IRI. Biochemical parameters, histopathological examination, and immunohistochemical markers were evaluated. A significant improvement in the biochemical, histopathological, and immunohistochemical parameters was observed in the DM + IRI + BC group, while the DM + IRI + Dex group showed improvements in renal IRI and dyslipidemia. The present study demonstrated that oxidative stress plays a chief role in renal IRI in the STZ-induced diabetic model. Treatment with BC achieved excellent ameliorative effects, while treatment with DEX improved renal IRI. Keywords: Diabetes; Dexmedetomidine; Ischemia/Reperfusion; Oxidative Stress

http://biomedpharmajournal.org/vol11no1/the-ameliorative-potential-of-dexmedetomidine-and-benincasa-cerifera-extract-in-renal-ischemiareperfusion-injury-in-a-streptozotocin-induced-diabetic-model/

View Article
March 2018

Impact Factor 0.200

3 Reads

Protective role of CoQ10 or L-carnitine on the integrity of the myocardium in doxorubicin induced toxicity.

Tissue Cell 2017 Jun 2;49(3):410-426. Epub 2017 Apr 2.

Pharmacology Department, National Research Center, Cairo, Egypt.

Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its clinical usage is hindered by the oxidative injury-related cardiotoxicity. This work aims to declare if the harmful effects of DOX on heart can be alleviated with the use of Coenzyme Q10 (CoQ10) or L-carnitine. The study was performed on seventy two female Wistar albino rats divided into six groups, 12 animals each: Control group; DOX group (10mg/kg); CoQ10 group (200mg/kg); L-carnitine group (100mg/kg); DOX+CoQ10 group; DOX+L-carnitine group. CoQ10 and L-carnitine treatment orally started 5days before a single dose of 10mg/kg DOX that injected intraperitoneally (IP) then the treatment continued for 10days. At the end of the study, serum biochemical parameters of cardiac damage, oxidative stress indices, and histopathological changes were investigated. CoQ10 or L-carnitine showed a noticeable effects in improving cardiac functions evidenced reducing serum enzymes as serum interleukin-1 beta (IL-1 ?), tumor necrosis factor alpha (TNF-?), leptin, lactate dehydrogenase (LDH), Cardiotrophin-1, Troponin-I and Troponin-T. Also, alleviate oxidative stress, decrease of cardiac Malondialdehyde (MDA), Nitric oxide (NO) and restoring cardiac reduced glutathione levels to normal levels. Both corrected the cardiac alterations histologically and ultrastructurally. With a visible improvements in ?-SMA, vimentin and eNOS immunohistochemical markers. CoQ10 or L-carnitine supplementation improves the functional and structural integrity of the myocardium.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tice.2017.03.007DOI Listing
June 2017
7 Reads
12 Citations
1.440 Impact Factor

Evaluation of the safety of conventional lighting replacement by artificial daylight.

J Microsc Ultrastruct 2017 Oct-Dec;5(4):206-215. Epub 2017 Jun 1.

Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, 51452 Buraydah, Saudi Arabia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jmau.2017.05.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025781PMC
June 2017
36 Reads

Prophylactic role of coenzyme Q10 and Cynara scolymus L on doxorubicin-induced toxicity in rats: Biochemical and immunohistochemical study.

Indian J Pharmacol 2015 Nov-Dec;47(6):649-56

Department of Pharmacology, National Research Center, Cairo, Egypt.

Objective: The study aims to evaluate the protective effects of coenzyme Q10 (CoQ10) and Cynara scolymus L (CS) on doxorubicin (dox)-induced toxicity.

Materials And Methods: Sixty male rats were divided into six groups. Group 1 as a control. Group 2 received dox (10 mg/kg) intraperitoneally. Group 3 received CoQ10 (200 mg/kg). Group 4 received CS (500 mg/kg). Group 5 received CoQ10 (200 mg/kg) and dox (10 mg/kg). Group 6 received CS (500 mg/kg) and dox (10 mg/kg). The rats were then evaluated biochemically and immunohistochemically.

Results: Dox produced a significant deterioration of hepatic and renal functional parameters. Moreover, an upsurge of oxidative stress and nitrosative stress markers. The expression of alpha-smooth muscle actin (?-SMA) was increased and proliferating cell nuclear antigen (PCNA) expression was decreased. Administration of CoQ10 and CS resulted in a significant improvement of hepatic and renal functional parameters, and an improvement of both ?-SMA and PCNA.

Conclusion: It is concluded that pretreatment with CoQ10 and CS is associated with up-regulation of favorable protective enzymes and down-regulation of oxidative stress. That can be advised as a supplement to dox-treated patients.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4103/0253-7613.169588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689020PMC
October 2016
8 Reads
21 Citations
0.902 Impact Factor

The role of curcumin in streptozotocin-induced hepatic damage and the trans-differentiation of hepatic stellate cells.

Authors:
Hesham N Mustafa

Tissue Cell 2016 Apr 9;48(2):81-8. Epub 2016 Feb 9.

Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address:

Diabetic patients frequently suffer from non-alcoholic steatohepatitis. The current study aimed to investigate the role of curcumin and the response of hepatic stellate cells in streptozotocin (STZ)-induced hepatic damage. Sixty male rats were divided into three groups. The normal control injected with a citrate buffer vehicle and the diabetic control group which was injected intraperitoneally (IP) with a single-dose of streptozotocin (50mg/kg body weight) and a diabetic group was treated with an oral dose of curcumin at 80 mg/kg body weight daily for 60 days. Curcumin effectively counteracts oxidative stress-mediated hepatic damage and improves biochemical parameters. Alpha-smooth muscle actin (?-SMA) was significantly reduced, and insulin antibodies showed strong positive immunoreactivity with curcumin administration. These results optimistically demonstrate the potential use of curcumin, which is attributed to its antiradical/antioxidant activities and its potential ?-cell regenerative properties. Also, it has the capability to encourage the trans-differentiation of hepatic stellate cells into insulin-producing cells for a period of time. In addition, as it is an anti-fibrotic mediator that inhibits hepatic stellate cell activation and the transition to myofibroblast-like cells, this suggests the possibility of considering curcumin's novel therapeutic effects in reducing hepatic dysfunction in diabetic patients.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tice.2016.02.003DOI Listing
April 2016
5 Reads
5 Citations
1.440 Impact Factor

Potential Alleviation of Chlorella vulgaris and Zingiber officinale on Lead-Induced Testicular Toxicity: an Ultrastructural Study.

Folia Biol (Krakow) 2015 ;63(4):269-78

Natural, products were studied to combat reproductive alterations of lead. The current work aimed to disclose the efficacy of Chlorella vulgaris and Zingiber officinale to alleviate lead acetate induced toxicity. Sixty adult male Wistar rats were distributed into four groups. Group 1 was considered control, group 2 received 200 mg/l PbAc water, group 3 received 50 mg/kg/rat of C. vulgaris extract and 200 mg/l PbAc water, and group 4 received 100 mg/kg/rat of Z. officinale and 200 mg/l PbAc water for 90 days. Testis samples were subjected to ultrastructural examination. It was observed that PbAc caused degenerative alterations in the spermatogenic series in many tubules, with a loss of germ cells and vacuoles inside the cytoplasm and between the germ cells. Mitochondria exhibited ballooning, with lost cristae and widening of the interstitial tissue, while nuclear envelopes of primary spermatocytes were broken up, and axonemes of the mid-pieces of the sperms were distorted. With the treatment with C. vulgaris or Z. officinale, there were noticeable improvements in these modifications. It was concluded that both C. vulgaris and Z. officinale represent convincing medicinal components that may be used to ameliorate testicular toxicity in those exposed to lead in daily life with superior potentials revealed by C. vulgaris due to its chelating action.

View Article

Download full-text PDF

Source
April 2016
7 Reads
5 Citations
0.523 Impact Factor

Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on cadmium induced testicular toxicity: an ultrastructural study.

Folia Morphol (Warsz) 2015;74(3):325-34

Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia..

Background: The current study aimed to elucidate the protective role of combined fenugreek and a-tocopherol against cadmium induced histopathological changes in the testes.

Materials And Methods: Thirty adult male albino rats divided into three equal groups 10 rats each. Group I is the control group. Group II received 5 mg/kg/ day cadmium chloride. Group III received 5 mg/kg/day cadmium chloride and 150 mg/kg/day fenugreek and 100 mg/kg/day of a-tocopherol. The treatment of all groups was done by oral gavage for 60 consecutive days. The testes were removed and subjected to histopathological and ultrastructure study.

Results: Rats exposed to cadmium showed severe histopathological changes in the testicular spermatogenic series, many vacuoles and multinucleated giant cells. Treatment with fenugreek and a-tocopherol partially improved the morphological changes, reduced tissue damage and rebuilt of the spermatogonia layer.

Conclusions: Fenugreek and a-tocopherol might represent a promising medicinal combination to ameliorate the toxic effects of cadmium exposure.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.5603/FM.2015.0049DOI Listing
January 2015
273 Reads
3 Citations
0.524 Impact Factor

The ameliorative potential of Hyphaene thebaica on streptozotocin-induced diabetic nephropathy.

Folia Morphol (Warsz) 2015;74(4):447-57

Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia..

Background: Diabetic nephropathy (DN) is the leading cause of end-stage renal disease. The aim of the current study is to investigate the possible beneficial effects of Hyphaene thebaica in DN.

Materials And Methods: For this, 50 male albino rats were divided into five groups: group I - represented the control group; group II - received Hyp-haene thebaica extracts of 150 mg/kg BW by oral gavage for 6 weeks; group III - received single intraperitoneal injections of streptozotocin (50 mg/kg BW) to induce type-2 diabetes mellitus; group IV (protective) - diabetic rats recei-ved Hyphaene thebaica extract (150 mg/kg BW) orally for 6 weeks; group V (curative) - received Hyphaene thebaica extract (150 mg/kg BW) orally after the diagnosis of DN.

Results: In the DN protected group, blood glucose, urea, and creatinine decreased significantly, while insulin and C-peptide increased significantly. Moreover, cystatin C and neutrophil gelatinase-associated lipocalin decreased. Collagen fibre deposition is increased with an apparent thickening of the parietal layer of Bowman's capsules and the basal lamina of convoluted tubules, as well as increase of the immune-reaction of caspase-3 and desmin. The introduction of Hyphaene thebaica led to greater amelioration in the biochemical markers, apoptotic alterations, and podocyte injuries of the protected group than in the curative group.

Conclusions: Hyphaene thebaica may be advised as a good choice that can delay diabetic renal complications.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.5603/FM.2015.0106DOI Listing
January 2015
5 Reads
4 Citations
0.524 Impact Factor

Effect of sodium selenite and vitamin E on the renal cortex in rats: an ultrastructure study.

Tissue Cell 2014 Jun 20;46(3):170-7. Epub 2014 Mar 20.

Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address:

This study examined the use of vitamin E to alleviate toxic effects of sodium selenite. Adult male albino rats (n=50) was divided into five groups. Group 1 was control, Groups 2 and 4 were treated with sodium selenite (2 mg/kg) for 2 and 4 weeks, respectively, Groups 3 and 5 were treated with sodium selenite (2 mg/kg) and vitamin E (100 mg/kg) for 2 and 4 weeks, respectively. Renal tissues were studied using anti-BCL2 and examined ultrastructurally. Positive Bax immunoreactivity was detected after 2 and more positive after 4 weeks and nearly all groups improved with co-administration of vitamin E. Ultrastructural study revealed lesions in Bowman's capsule and proximal convoluted tubules. The submicroscopic study revealed damage and necrosis of cortical structures after 2 and 4 weeks, respectively. After 4 weeks, cellular changes were seen, such as vacuolation and moderate degeneration of cells, widening of the urinary space scattered through the cortex with loss of cellular details, formation of apical buds, degeneration, and cellular rupture. Present findings disclosed an ameliorative effect of adding vitamin E to sodium selenite-induced changes in cortical tissues. Clinically, it is advised to add vitamin E to avoid selenium overdose hazards.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tice.2014.03.002DOI Listing
June 2014
9 Reads
13 Citations
1.440 Impact Factor

The effects of diethylstilbestrol administration on rat kidney. Ultrastructural study.

Saudi Med J 2013 Nov;34(11):1114-24

Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.

Objective: To assess the histological and ultrastructural changes that can be induced by diethylstilbestrol (DES) on renal tissues using histological, immunohistochemical, and ultrastructural methods.

Methods: Thirty adult male Wistar rats were divided into 3 groups (10 rats each): Group 1 - control; Group 2 - received DES at a dose of 60 ug/kg/day, dissolved in 0.1 ml corn oil for 20 days; and Group 3 - received the same dose of DES for 50 days by oral gavage. The renal tissues were studied histologically, immunohistochemically (using an anti-BCL2-associated X protein [BAX protein] antibody), and ultrastructurally. This study was carried out at the Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia between December 2011 and December 2012.

Results: The DES administration for 50 days caused noticeable degeneration, and alteration of the morphology of the renal tissues in the form of damaged renal tubules with loss of the brush border of the proximal convoluted tubules and increased cellularity of the glomeruli. In addition, there was a significant increase in BAX protein expression based on immunoreactivity, and in renal tubules, as well as glomerular cells. These changes were less obvious after 20 days of treatment.

Conclusion: Non-steroidal, synthetic estrogens showed harmful effects on the renal tissues and altered their morphology with an increased number of apoptotic cells, and these changes were duration dependent.

View Article

Download full-text PDF

Source
November 2013
7 Reads
3 Citations
0.554 Impact Factor

Protective role of antioxidants on thioacetamide-induced acute hepatic encephalopathy: biochemical and ultrastructural study.

Tissue Cell 2013 Oct 19;45(5):350-62. Epub 2013 Jul 19.

Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.

Thioacetamide (TAA) has been used in development of animal models of acute hepatic encephalopathy (AHE). This experimental study was designed to evaluate effects of oral administration of vitamin C, vitamin E and their combination on liver and brain enzymes and their histologic and ultrastructure changes. Eighty Wistar rats were included and divided into five groups (16 each). Group 1 (control) received saline once intraperitoneally (IP) then administered orally saline and corn oil for 3 days. Group 2 [hepatotoxic (TAA)] were received TAA (300mg/kg) once intraperitoneally (IP). Group 3 (vitamin C and TAA) received TAA (300mg/kg) once intraperitoneally (IP) and then administered orally vitamin C (100mg/kg) daily for 3 days. Group 4 (vitamin E and TAA) received TAA (300mg/kg) once intraperitoneally (IP) and then administered orally vitamin E (200mg/kg) daily for 3 days. Group 5 (vitamin C and vitamin E and TAA) received TAA (300mg/kg) once intraperitoneally (IP) and then administered orally vitamin C (100mg/kg) in combination with vitamin E (200mg/kg) daily for 3 days. All rats were sacrificed 24h after last treatment under anesthesia. Blood samples were collected and serum was obtained for analysis of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), total protein, triglyceride, cholesterol using spectrophotometer and ELISA kits. Liver and brain were extracted and tissue homogenate was used to measure malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide (NO). Histological and ultrastructure examination were done. TAA induced significant increase of MDA and decreased in GSH and NO in both liver and brain homogenate with more liver affection, and increased in serum levels of AST, ALT, triglyceride, cholesterol and decreased in total protein. Furthermore, there is decrease in serum levels of AST, ALT, triglyceride, cholesterol and tissue levels of MDA and elevated serum total protein and tissue GSH and NO under the umbrella of vitamin C and vitamin E and their combination, although vitamin E is more efficient. These data showed protective effect of vitamins C and E, especially vitamin E against oxidative stress and hepatic and brain damage, and histological architecture of the liver in rats' model of acute hepatic encephalopathy elicited by TAA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tice.2013.06.001DOI Listing
October 2013
95 Reads
29 Citations
1.440 Impact Factor

Effect of acrylamide on testis of albino rats. Ultrastructure and DNA cytometry study.

Authors:
Hesham N Mustafa

Saudi Med J 2012 Jul;33(7):722-31

Department of Anatomy, Faculty of Medicine, King Abdulaziz University, PO Box 80205, Jeddah 21589, Kingdom of Saudi Arabia.

Objective: To explore the harmful effects of acrylamide on the structure of testis in albino rats, in an attempt to clarify its potential risks on human health.

Methods: The present study was carried out in the Department of Anatomy, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia from December 2010 to December 2011. Forty-eight adult male albino rats (250-300 g) were divided randomly into 6 groups. Electron microscopy and histochemical techniques using Feulgen stain were used to conduct the morphological study. In addition, DNA cytometry method was used.

Results: Rats treated with acrylamide 25 mg/kg body weight for 10 days showed mild affection, whether acrylamide was administered orally or intraperitoneally. On the other hand, the testis of the group treated with a dose of 50 mg/kg/10 days showed damage, especially with intraperitoneal administration in comparison to oral treatment. This was in the form of degeneration of germ cells, numerous multinucleated giant cells with sloughed seminiferous epithelium, and vacuolation in-between the germ cells.

Conclusion: Exposure to acrylamide produced degenerative changes in the testis, which were more prominent with a longer period of exposure. Recommendations are necessary to decrease acrylamide level in different foods, and ways to decrease acrylamide formation during preparation of different foods should be advertised.

View Article

Download full-text PDF

Source
July 2012
6 Reads
9 Citations
0.554 Impact Factor

Top co-authors

Gehan A Hegazy
Gehan A Hegazy

King Abdulaziz University

3
Mustafa H N
Mustafa H N

Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.

1
Hamid A Saleh
Hamid A Saleh

King Abdulaziz University

1
Mohammed Farahna
Mohammed Farahna

Qassim University

1

Followers