Publications by authors named "Henning Feist"

15 Publications

  • Page 1 of 1

Pulmonary echinococcosis: A rare pseudotumour of the lung.

Rare Tumors 2021 11;13:20363613211009769. Epub 2021 Apr 11.

Institut für Pathologie, DIAKO Hospital Flensburg, Flensburg, Germany.

Cystic echinococcosis is a widely endemic helminthic disease worldwide but occurs only rarely in Central Europe. Humans are infected as 'aberrant' hosts by and develop cysts in numerous different organs. 20%-30% of the affected individuals develop hydatid disease in the lungs with associated complications including pleuritis, lung abscess and pneumothorax. Radiologically, the pulmonary lesions of cystic echinococcosis occasionally pose difficulties in the differential diagnosis of primary lung carcinoma or metastatic disease and vice versa. Herein we report on a case of pulmonary hydatid disease in a 25-year-old Iraqi male presenting with a cystic lesion of the lung associated with thoracic pain and involuntary weight loss. Despite of its rare occurrence in Central Europe, clinicians, radiologists and pathologists should be aware of this entity and its pulmonary manifestations. During frozen section examination, imprint cytology specimens may facilitate the detection of the pathogens.
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http://dx.doi.org/10.1177/20363613211009769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044558PMC
April 2021

Villitis of unknown etiology and chronic deciduitis are not associated with human papilloma virus and enterovirus infection.

Virchows Arch 2020 Jul 5;477(1):73-81. Epub 2020 Feb 5.

Department of Virology, Medical School Hannover, Hannover, Germany.

Villitis of unknown etiology (VUE) and chronic deciduitis with plasma cells (CD) are supposed to be non infectious placental lesions caused by a pathologic immune reaction similar to a host versus graft mechanism. In some investigations, infection of human trophoblastic cells with human papilloma virus (HPV) has been described, and a relationship with miscarriage, preeclampsia, and chronic inflammatory placental lesions has been suspected. Infection with enterovirus, especially Coxsackievirus, has been observed in cases with spontaneous abortion and adverse perinatal outcome, respectively. We investigated 20 cases with VUE and 30 cases with chronic deciduitis with plasma cells. The placenta specimens were analyzed for expression of HPV capsid protein by immunohistochemistry, for presence of HPV DNA via polymerase chain reaction (PCR), and for presence of enterovirus mRNA using RT-PCR, respectively. VUE was associated with maternal diseases: atopic lesions in 21%, other autoimmune diseases in 15.5%, and obesity in 31.5%, respectively. Birth weight below the 10th percentile was detected in 63% of the cases with VUE. Chronic deciduitis was associated with preterm labor and preterm premature rupture of membranes (26%). Intrauterine fetal demise occurred in 5 cases with CD (18.5%). HPV DNA, HPV capsid protein, and enterovirus mRNA were not detected in all investigated VUE or CD cases. Our investigations show that a causal role for enterovirus and human papilloma virus in the development of VUE and CD is unlikely. Therefore, HPV vaccination is unlikely to reduce the incidence of VUE and CD in the future.
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http://dx.doi.org/10.1007/s00428-020-02765-0DOI Listing
July 2020

Pathoanatomical Lesions in Placentas With Excessively Hypercoiled Umbilical Cords: Frequent Detection of Massive Perivillous Fibrin Deposition.

Pediatr Dev Pathol 2020 Mar-Apr;23(2):107-114. Epub 2019 Jul 25.

Department of Pathology, Diakonissenkrankenhaus Flensburg, Flensburg, Germany.

This study focused to investigate a possible association of extensive umbilical hypercoiling (displaying an umbilical coiling index [UCI] of at least 1.0 coils/cm), clinical outcome, and associated pathoanatomical placental lesions. Of the 771 singleton placentas from the second and third trimesters submitted for pathoanatomical evaluation, 15 cases (2%) displayed extensive hypercoiling. There was an association of excessive hypercoiling with hypotrophy of fetuses and children (11 cases) and fetal demise (12 cases). Thin cord syndrome and umbilical stricture were observed in 9 cases and 4 cases, respectively. Seven of the 15 cases with excessive umbilical hypercoiling showed increased placental fibrin deposition (47% of the cases with hypercoiling), in 4 cases sufficient for rendering the diagnosis of massive perivillous fibrin deposition. Signs of maternal vascular malperfusion (n = 6) and chorangiosis (n = 2) were also detected in cases with hypercoiling. Recurrence of excessive umbilical hypercoiling was observed in 2 families, suggesting a genetic predisposition for the development of this lesion. Extensive hypercoiling could be a hitherto underrecognized pathogenetic factor for the development of massive perivillous fibrin deposition. A high UCI measured in the second trimester by ultrasound may be predictive of fetal hypotrophy, and intensified fetal monitoring is warranted, particularly if there is a history of hypercoiling and adverse fetal outcome.
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http://dx.doi.org/10.1177/1093526619865426DOI Listing
January 2021

Discordancy for a Villous Maturation Defect in a Dizygotic Twin Placenta.

Fetal Pediatr Pathol 2019 Oct 26;38(5):432-436. Epub 2019 Apr 26.

Department of Pathology/Pediatric Pathology, University of Cologne , Cologne , Germany.

Dizygotic twin pregnancies with discordant manifestation of abnormalities with unclear etiology are of interest because they arise in the same environment. We present a dizygotic third trimester twin placenta with discordant villous maturation, one placenta lacking developed syncytiocapillary membranes. The twins were eutrophic with no perinatal or postnatal complications. Discordant manifestation of villous maturation in dizygotic twin placentas could be a hint for a genetic rather than an environmental etiology. Villous maturation defect may be underrecognized and has been associated with perinatal morbidity and stillbirth in the late third trimester. Proper recognition is important because of the increased recurrence risk of villous dysmaturity.
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http://dx.doi.org/10.1080/15513815.2019.1604924DOI Listing
October 2019

Complement receptor-associated CD163/CD18/CD11c/CD206/CD209 expression profile in chronic histiocytic intervillositis of the placenta.

Placenta 2019 03 15;78:23-28. Epub 2019 Feb 15.

Institut für Pathologie, Ev.-Luth. Diakonissenanstalt zu Flensburg, Knuthstr. 1, 24939, Flensburg, Germany.

Introduction: Chronic histiocytic intervillositis of unknown etiology (CIUE) is a non-infectious, most probably immunologic placenta lesion. CIUE is associated with recurrent miscarriage, intrauterine growth restriction and stillbirth. Among the pathologic-anatomic defined placental lesions this entity displays the highest risk of recurrence in following pregnancies (about 67-100%). The histiocytic cells accumulate in the placental blood space but do not infiltrate into the villi or decidua. Sparsely known is the expression profile of these intervillous cells regarding histiocytic markers.

Methods: We analysed 5-22 markers by immunohistochemistry in a total of 41 placenta samples and evaluated decidual, villous and intervillous histiocytic cells.

Results: In CIUE, intervillous CD163 histiocytes over-express CD11c/CD18 and down-regulate CD206/CD209, while CD163 decidual and Hofbauer cells show low CD11c/CD18 and higher CD206/CD209 protein expressions.

Discussion: CD163 expression indicates a M2-like polarisation. CD11c and CD18 form the complement receptor 4 which could be related to a complement mediated trigger for aberrant cell accumulation in CIUE.
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http://dx.doi.org/10.1016/j.placenta.2019.02.007DOI Listing
March 2019

Massive Perivillous Fibrin Deposition of an Enterovirus A-Infected Placenta Associated With Stillbirth: A Case Report.

Pediatr Dev Pathol 2019 Mar-Apr;22(2):142-145. Epub 2018 Sep 7.

5 Department of Virology, Hannover Medical School, Hannover, Germany.

Massive perivillous fibrin deposition (MFD) is a morphologically defined severe placental lesion associated with perinatal morbidity and mortality. The etiology is unknown, and recurrence risk in subsequent pregnancies is assumed to be high. In most cases, a pathologic immune reaction is supposed to be responsible for the lesion. We report a case of a pregnant woman's suffering from hand, foot, and mouth disease in the 20th gestational week. Subsequently, MFD developed in the placenta and was followed by intrauterine growth restriction and stillbirth in the 29th gestational week. Enterovirus A with high homology to Coxsackievirus A16 was detected in the placenta by means of immunohistochemisty and reverse transcription polymerase chain reaction. This infection could be a rare cause of MFD and should be taken into consideration in the differential diagnosis of the individual etiology. Recurrence risk of virus-related MFD is expected to be lower than in MFD without infectious association.
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http://dx.doi.org/10.1177/1093526618798772DOI Listing
April 2019

Papilloma of the Fallopian Tube: A Rare Gynecologic Neoplasm Harboring a BRAF (c.1799T>A) Mutation (V600E).

Int J Gynecol Pathol 2019 Sep;38(5):459-463

Institute of Pathology, University Hospital of Essen, University of Duisburg-Essen, Essen (J.W., K.W., T.H., K.W.S) Institute of Pathology (J.W., H.F., J.P.) Department of Gynecology and Obstetrics (H.O.), Lutheran Diakonissen-Hospital Flensburg, Flensburg, Germany Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital and Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway (B.D.).

Papillomas of the fallopian tube are exceedingly rare benign tumors, and only very few cases have been reported in the literature. Clinically, they may present as a mass lesion or occur without symptoms. Histomorphologically, they are papillary tumors covered by nonatypical epithelium with occasional ciliated or goblet cells growing in the lumen, and they are most frequently located in the infundibular region of the fallopian tube. They require a number of differential diagnostic evaluations and can be mistaken for either other benign tumors or malignant neoplasms. Because of their rare occurrence, molecular data about this entity have been lacking so far. Herein, a case of a papilloma with a BRAF (c.1799T>A) mutation (V600E) in a 45-yr-old woman with tumor-like dilation of the fallopian tube is presented.
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http://dx.doi.org/10.1097/PGP.0000000000000526DOI Listing
September 2019

Expression of Toll-Like Receptors in Chronic Histiocytic Intervillositis of the Placenta.

Fetal Pediatr Pathol 2015 26;34(6):407-12. Epub 2015 Oct 26.

b Diakonissenkrankenhaus Flensburg , Flensburg , Germany.

Chronic histiocytic intervillositis of the placenta (CHI) shows monocytic/histiocytic infiltration of the intervillous space. Placental malaria has a CHI-like histopathology and induces an aberrant expression of Toll-like receptors (TLR) 3, 7-9. We hypothesized that, similar to placental malaria, CHI could be associated with increased TLR expression. TLR1-10 and other inflammation-associated factors were analyzed by real-time PCR and immunohistochemistry. A total of 31 formalin-fixed and paraffin-embedded placenta samples were evaluated: CHI (n = 9), and for control purposes, villitis of unknown etiology (VUE, n = 8) and placentas without inflammation (n = 14). CHI shows increased expression of monocytic TLR1, a receptor which is involved in bacterial lipopolysaccharide (LPS)-induced inflammation. This could indicate a TLR1-mediated immune mechanism in the placenta (e.g. triggered by transient, clinically inapparent maternal bacteraemia) which leads to massive monocytic/histiocytic accumulation in the intervillous space. The increased expression of TLR1 with no increased expression of TLR3 and TLR7-9 is different from that in malaria.
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http://dx.doi.org/10.3109/15513815.2015.1095259DOI Listing
August 2016

Discordancy for Placental Massive Perivillous Fibrin Deposition and Fetal Growth in Dichorionic Twins after In Vitro Fertilization.

Pediatr Dev Pathol 2015 Sep-Oct;18(5):405-9. Epub 2015 Apr 23.

4 Department of Pathology, Medical School, Hannover, Germany.

Massive perivillous fibrin deposition (MFD) is a placental lesion of unknown etiology associated with perinatal morbidity and mortality and recurrence risk in subsequent pregnancies. We report a 34 weeks' gestation dizygotic twin pregnancy with discordancy for MFD and for intrauterine growth restriction after in vitro fertilization (IVF). Only the smaller twin corresponding to the placenta affected by MFD showed intrauterine growth restriction, with a weight below the 3rd percentile according to gestational age. The affected placenta showed a moderate increase in decidual lymphocytes. No difference in expression of complement factor C4d in umbilical veins could be observed. Development of MFD in one of the dizygotic placentas may be due to a pathologic immune response to one of the different fetal genotypes as semiallografts. The pathogenetic role of IVF as an environmental factor for development of MFD is unclear.
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http://dx.doi.org/10.2350/15-02-1609-CR.1DOI Listing
February 2016

Partial hydatidiform mole with extensive angiomatoid vessel configuration in a first trimester miscarriage.

Int J Gynecol Pathol 2015 May;34(3):253-6

Institute of Pathology (H.F.) Department of Obstetrics and Gynecology (J.O.), Diakonissenkrankenhaus Flensburg, Flensburg Department of Human Genetics (A.C.), University of Kiel, Kiel Institute of Pathology of the Charite (N.S.), Humboldt-University, Berlin Institute of Pathology (K.H.), Medical School, Hannover, Germany.

We report a first trimester miscarriage (9 wk gestation) with a macroscopic grape-like aspect due to extensive angiomatoid changes with widened communicating thin-walled villous vessels. Fluorescence in situ hybridization analysis and microsatellite analysis revealed a diandric triploidy of the trophoblastic tissue, so this miscarriage is indeed a genetic partial hydatidiform mole. This is remarkable since the typical morphologic hallmarks of partial hydatidiform mole, especially enhanced trophoblastic proliferation and marked villous cistern formation, were not prominent. The finding of extensive angiomatoid morphology is to our knowledge an undescribed morphology of an early partial hydatidiform mole. It serves as an example of the morphologic variability of this probably underestimated condition that has a slightly elevated risk for the development of gestational trophoblastic disease.
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http://dx.doi.org/10.1097/PGP.0000000000000161DOI Listing
May 2015

Array-CGH and quantitative PCR genetic analysis in a case with bilateral hypoplasia of pulmonary arteries and lungs and simultaneous unilateral renal agenesis.

Int J Clin Exp Pathol 2010 Aug 18;3(7):723-9. Epub 2010 Aug 18.

Institute of Pathology, Hannover Medical School, 30625 Hannover, Germany.

We describe the clinical course and have characterised anatomically and genetically a unique case of a newborn with bilateral hypoplasia of pulmonary arteries, consecutive extremely hypoplastic lung tissue and associated unilateral renal agenesis. Intrauterine oxygenation by the placenta seemed to have allowed normotrophic body maturity but immediately after delivery, in the third trimester, progressive hypoxemia developed and the newborn succumbed to acute respiratory failure. Genetic analysis by array-based comparative genomic hybridisation and quantitative PCR revealed duplication of 1p21, which, however, might not be the disease causing aberration. This case might represent an extreme form of previously reported, rare cases with simultaneous dysorganogenesis of lungs and kidneys.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2933393PMC
August 2010

Conversion of a neonatal hepatic hemangioma to focal nodular hyperplasia.

Pathol Int 2009 Apr;59(4):251-4

Department of Pediatric Surgery, Hannover Medical school, Hannover, Germany.

Hepatic hemangioma and focal nodular hyperplasia are both frequently observed benign lesions of the liver. Whereas hepatic hemangioma is the most frequent benign liver tumor in children, focal nodular hyperplasia occurs predominantly in adult patients. Concomitance of both entities has been described in adults, suggesting a similar pathogenesis. We report on a 6-month-old child with a continuously shrinking hepatic hemangioma after interventional therapy and a growing hepatic mass 5 years later, which emerged as focal nodular hyperplasia at the site of the former hemangioma. Diagnostic and therapeutic strategies regarding this patient are discussed. The present case supports the theory that these two entities may share a similar pathomechanism.
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http://dx.doi.org/10.1111/j.1440-1827.2009.02359.xDOI Listing
April 2009

[Necrotizing sialometaplasia: diagnosis of a rare entity].

Schweiz Monatsschr Zahnmed 2008 ;118(7):635-40

Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Medizinische Hochschule Hannover, 30625 Hannover, Deutschland.

A differential diagnosis of an ulcer on the hard palate must consider a wide variety of diseases and conditions, among them rare entities such as necrotizing sialometaplasia. We report the case of a patient who presented with a painful ulcer on the hard palate. A biopsy taken at the initial presentation of the patient revealed a diagnosis of necrotizing sialometaplasia. Histology showed foci of eosinophilic granulocytes with lobular infarction or necrosis, bland-appearing nuclear morphology of squamous cells, simultaneous metaplasia of ducts and mucous acini. The ulcer resolved spontaneously within seven weeks and required no specific treatment. The case presented here shows that early diagnosis of necrotizing sialometaplasia is important in order to prevent unnecessary treatment.
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September 2008

SELDI-MS-based expression profiling of ductal invasive and lobular invasive human breast carcinomas.

Pathol Res Pract 2005 24;201(12):763-70. Epub 2005 Oct 24.

Institute of Pathology, Medizinische Hochschule Hannover, Carl-Neuberg-Street 1, 30635 Hannover, Germany.

Expression profiling using proteomic techniques has a great potential to identify new biomarkers that might help to better diagnose and treat diseases such as breast cancer, which is one of the leading causes of cancer death in women. Surface-enhanced laser desorption ionization mass spectrometry (SELDI-MS) combines chromatographic separation of peptides and proteins with mass spectrometry and is a fast, user-friendly tool to analyze protein and peptide profiles. SELDI-MS was employed for a comparative analysis of lobular invasive versus ductal invasive breast tumors to find differentially expressed proteins and peptides, and to validate this technique for biomarker identification using complex samples such as tissue. After optimization of sample preparation using HMEC and MCF-7 cell lines, 20 breast tumors were analyzed, and about 550 mass signals corresponding to an estimated 140 native peptides and proteins were detected in each tumor. Only 14% of the mass signals were present in more than six tumors of one subgroup or in more than 12 tumors of both groups showing a great overall heterogeneity of the peptide and protein profiles obtained. Peptide mass signals specific for each of the analyzed groups were identified. In addition, we detected peptides from laser-microdissected ductal invasive and intraductal tumor parts corresponding to peptides present in whole tumors. The low amount of identified peptides and proteins and the observed heterogeneity suggest that SELDI-MS is not well suited for biomarker identification of and profiling experiments on complex samples such as tumor tissue.
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http://dx.doi.org/10.1016/j.prp.2005.08.011DOI Listing
May 2006

Quantitative assessment of promoter hypermethylation during breast cancer development.

Am J Pathol 2002 Feb;160(2):605-12

Institute of Pathology, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany.

The aberrant methylation of cytosine residues in the promoter region of growth regulatory genes is now widely recognized as an additional mechanism for gene inactivation in cancer cells. In this study we analyzed the methylation status of four growth regulatory genes (p16, RASSF1A, cyclinD2, 14-3-3zeta) during breast cancer progression. For this purpose invasive and noninvasive tumor cell populations as well as hyperplastic cell proliferations were isolated from a series of archival breast tissue specimens (n = 57) using laser-assisted microdissection. A new real-time polymerase chain reaction-based assay was used for the sensitive and quantitative determination of the cell-specific methylation status. We found that aberrant promoter methylation was already prevalent in pure intraductal carcinoma with different frequencies and different methylation levels for the four genes analyzed. For RASSF1A and 14-3-3zeta promoter methylation was also demonstrated in epithelial hyperplasia and intraductal papillomas. By contrast, aberrant methylation of cyclinD2 and p16 was restricted to cancerous epithelium. Increased methylation of the cyclinD2 gene was significantly associated with a higher van Nuys grade. Furthermore, when intraductal and invasive tumor cells were compared, significant quantitative changes in the methylation level were detected primarily within the cyclinD2 gene. These results demonstrate that promoter methylation is an early and frequent event in breast cancer development, but displays great quantitative and gene-specific differences, and changes in a gene-specific manner during tumor progression.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1850646PMC
http://dx.doi.org/10.1016/S0002-9440(10)64880-8DOI Listing
February 2002