Publications by authors named "Heng Zhang"

1,629 Publications

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Pan-cancer analysis of microbiome quantitative trait loci.

Cancer Res 2022 Aug 16. Epub 2022 Aug 16.

Wuhan University, China.

Microorganisms are commonly detected in tumor tissues, and the species and abundance have been reported to affect cancer initiation, progression, and therapy. Host genetics have been associated with gut microbial abundances, while the relationships between genetic variants and the cancer microbiome still require systematic interrogation. Therefore, identification of cancer microbiome quantitative trait loci (mbQTL) across cancer types might elucidate the contributions of genetic variants to tumor development. Using genotype data from The Cancer Genome Atlas and microbial abundance levels from Kraken-derived data, we developed a computational pipeline to identify mbQTLs in 32 cancer types. This systematically identified 38,660 mbQTLs across cancers, ranging 50 in endometrial carcinoma to 3,133 in thyroid carcinoma. Furthermore, a strong enrichment of mbQTLs was observed among transcription factor binding sites and chromatin regulatory elements, such as H3K27ac. Notably, mbQTLs were significantly enriched in cancer GWAS loci and explained an average of 2% of cancer heritability, indicating that mbQTLs could provide additional insights into cancer etiology. Correspondingly, 24,443 mbQTLs overlapping with GWAS linkage disequilibrium regions were identified. Survival analyses identified 318 mbQTLs associated with patient overall survival. Moreover, we uncovered 135,248 microbiome-immune infiltration associations and 166,603 microbiome-drug response associations that might provide clues for microbiome-based biomarkers. Finally, a user-friendly database, Cancer-mbQTL (http://canmbqtl.whu.edu.cn/#/), was constructed for users to browse, search, and download data of interest. This study provides a valuable resource for investigating the roles of genetics and microorganisms in human cancer.
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http://dx.doi.org/10.1158/0008-5472.CAN-22-1854DOI Listing
August 2022

[Remediation Potential of Rodin on Lead and Cadmium Contaminated Farmland Soil].

Huan Jing Ke Xue 2022 Aug;43(8):4253-4261

College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, China.

Rodin (TSK) is an energy plant that can be used as a raw material for natural rubber. The aim of this study was to examine the remediation potential of TSK to lead (Pb)- and cadmium (Cd)-contaminated farmland soil. In this study, a pot experiment was conducted, and the "Soil Environmental Quality and Agricultural Land Soil Pollution Risk Control Standard (GB 15618-2018)" was used as reference. We set up four different concentrations of Pb and Cd pollution treatment to study the characteristics of the accumulation and tolerance of TSK to Pb and Cd. The results showed that as the content of Pb and Cd in the soil increased, the chlorophyll content and biomass of TSK gradually decreased, and the SOD, POD, and CAT enzyme activities gradually increased. The BCF and TF of Cd were between 1.20 and 1.50, indicating that TSK presented some characteristics of a Cd hyperaccumulator. The BCF and TF of Pb were between 0.71 and 1.11. Thus, TSK was a good Pb enrichment plant and possessed the potential to repair soil with (Pb) below 400 mg·kg. The accumulation of Pb and Cd gradually increased, and the maximum accumulation of Cd and Pb in the shoots was 9.832 μg·plant and 1091.185 μg·plant, respectively. However, in lower concentrations of Pb- and Cd-contaminated soil, the removal rate was greater, and the remediation efficiency was better. Overall, using TSK to repair Pb- and Cd-contaminated farmland soil has good application prospects and economic value.
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http://dx.doi.org/10.13227/j.hjkx.202110142DOI Listing
August 2022

Multiple Lesions at Different Stages of Pyoderma Gangrenosum in a Crohn's Disease Patient.

Clin Cosmet Investig Dermatol 2022 9;15:1593-1596. Epub 2022 Aug 9.

Department of Dermatology, Peking University People's Hospital, Beijing, 100044, People's Republic of China.

Pyoderma gangrenosum (PG) is an inflammatory dermatosis characterized by the rapid progression of a painful, necrolytic ulcer with an irregular and undermined border. The prevalence of PG in patients with Crohn's disease (CD) has been estimated to be 0.7%. Here, we report a case that presented various painful skin lesions, including erythema, vesicles, plaques, and ulcers, one week before the fourth infliximab infusion for CD. PG was diagnosed and the lesions subsided after a 390-mg ustekinumab infusion for one month. It suggests that different lesions of PG may occur concomitantly in CD patients, and the therapy should be re-evaluated on time.
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http://dx.doi.org/10.2147/CCID.S374973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375554PMC
August 2022

oligosaccharides increase serotonin in the brain and ameliorate depression promoting 5-hydroxytryptophan production in the gut microbiota.

Acta Pharm Sin B 2022 Aug 3;12(8):3298-3312. Epub 2022 Mar 3.

National Engineering Research Center for the Emergence Drugs, Beijing 100000, China.

oligosaccharides (MOO) are an oral drug approved in China for the treatment of depression in China. However, MOO is hardly absorbed so that their anti-depressant mechanism has not been elucidated. Here, we show that oral MOO acted on tryptophan → 5-hydroxytryptophan (5-HTP) → serotonin (5-HT) metabolic pathway in the gut microbiota. MOO could increase tryptophan hydroxylase levels in the gut microbiota which accelerated 5-HTP production from tryptophan; meanwhile, MOO inhibited 5-hydroxytryptophan decarboxylase activity, thus reduced 5-HT generation, and accumulated 5-HTP. The raised 5-HTP from the gut microbiota was absorbed to the blood, and then passed across the blood-brain barrier to improve 5-HT levels in the brain. Additionally, pentasaccharide, as one of the main components in MOO, exerted the significant anti-depressant effect through a mechanism identical to that of MOO. This study reveals for the first time that MOO can alleviate depression increasing 5-HTP in the gut microbiota.
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http://dx.doi.org/10.1016/j.apsb.2022.02.032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366226PMC
August 2022

Designer Cathode Additive for Stable Interphases on High-Energy Anodes.

J Am Chem Soc 2022 Aug 12. Epub 2022 Aug 12.

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

Rechargeable lithium-based batteries built with high-energy anode materials (e.g., silicon-based and silicon-derivative materials) are considered a feasible solution to satisfy the stringent requirements imposed by emerging markets, including electric vehicles and grid storage, due to their higher energy density compared to contemporary lithium-ion batteries. The robustness of the solid electrolyte interphase (SEI) layer on high-energy anodes is critical to achieve long-term and stable cycling performances of the batteries. Herein, we propose a new type of designer cathode additive (DCA), i.e., an ultrathin coating layer of elemental sulfur on the cathode, for the in situ formation of a thin and robust SEI layer on various types of high-energy anodes. The DCA elemental sulfur undergoes simultaneous oxidation and reduction paths, forming lithium alkyl sulfate (R-OSOOLi) and poly(ethylene oxide) (PEO)-like polymers on the anode surface. The as-formed R-OSOOLi/PEO-modified SEI layer has good lithium cation (Li) permeability to facilitate fast ion transportation across the interphases and superior elasticity to adapt to large volume changes, which is particularly effective for improving the cycling efficiency of high-energy anodes (e.g., ca. 14-35% increase in capacity retention for the silicon-carbon composite (SiC) or silicon-tin alloy (Si-Sn)||LiFePO cells). The present work opens a new avenue toward the practical deployment of high-energy rechargeable lithium-based batteries.
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http://dx.doi.org/10.1021/jacs.2c04124DOI Listing
August 2022

Perspectives from remote sensing to investigate the COVID-19 pandemic: A future-oriented approach.

Front Public Health 2022 26;10:938811. Epub 2022 Jul 26.

Faculty of Animal Husbandry, Institute of Animal and Dairy Sciences, University of Agriculture, Faisalabad, Pakistan.

As scientific technology and space science progress, remote sensing has emerged as an innovative solution to ease the challenges of the COVID-19 pandemic. To examine the research characteristics and growth trends in using remote sensing for monitoring and managing the COVID-19 research, a bibliometric analysis was conducted on the scientific documents appearing in the Scopus database. A total of 1,509 documents on this study topic were indexed between 2020 and 2022, covering 165 countries, 577 journals, 5239 institutions, and 8,616 authors. The studies related to remote sensing and COVID-19 have a significant increase of 30% with 464 articles. The United States (429 articles, 28.42% of the global output), China (295 articles, 19.54% of the global output), and the United Kingdom (174 articles, 11.53%) appeared as the top three most contributions to the literature related to remote sensing and COVID-19 research. , and were the three most productive journals in this research field. The utmost predominant themes were COVID-19, remote sensing, spatial analysis, coronavirus, lockdown, and air pollution. The expansion of these topics appears to be associated with cross-sectional research on remote sensing, evidence-based tools, satellite mapping, and geographic information systems (GIS). Global pandemic risks will be monitored and managed much more effectively in the coming years with the use of remote sensing technology.
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http://dx.doi.org/10.3389/fpubh.2022.938811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360797PMC
August 2022

A Universal Calibration Device for an Air Flow Sensor of the VAV Terminal Unit.

Sensors (Basel) 2022 Aug 3;22(15). Epub 2022 Aug 3.

College of Metrology and Measurement Engineering, China Jiliang University, Hangzhou 310018, China.

In this paper, a new calibration device for an air flow sensor of the VAV terminal unit is designed. Multi-aperture air outlets are designed to meet the calibration requirements of the air flow sensor in a variety of measurement range. The device can calibrate the air flow sensors of different types of VAV terminal unit by a movable flow rectifier without repeating the design of a different calibration pipeline. The Raspberry PI is used to design the high-performance GUI interface and controlling algorithm to achieve a one-button intelligent calibration. The air flow sensors in three different types of VAV terminal units are used to calibrate the experiment. After testing, the differential pressure value measured by the air flow sensor can accurately measure the air flow within the accuracy of 5% after the formula conversion. The conversion from differential pressure values to air flow values requires precise calibration in order to establish an accurate air flow equation, and here the calibration device plays a key role. The negative effect caused by the distance between the flow rectifiers and the VAV terminal unit is discovered. In other words, the distance between the inlet flow rectifier and the air inlet of VAV terminal unit should be kept as close as possible, or within a range of 2~3 cm. Moreover, the distance between the air outlet of VAV terminal unit and the middle-flow rectifier should be kept as close as possible; otherwise, any slight gap will cause a huge error in the calibration result. The research contributes to the further study of airflow sensing technology through the conversion and calibration of differential pressure measurements to accurate air flow values.
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http://dx.doi.org/10.3390/s22155797DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371138PMC
August 2022

Two-Dimensional Simulation of the Freezing Characteristics in PEMFCs during Cold Start Considering Ice Crystallization Kinetics.

Polymers (Basel) 2022 Aug 5;14(15). Epub 2022 Aug 5.

School of Automotive Engineering, Wuhan University of Technology, Wuhan 430070, China.

Cold start is one of the major issues that hinders the commercialization of polymer electrolyte membrane fuel cells (PEMFCs). In this study, a 2D transient multi-physics model is developed to simulate the cold start processes in a PEMFC. The phase change between water vapor, liquid water, and ice in the catalyst layers (CLs), micro porous layer (MPLs), and gas diffusion layers (GDLs) is also investigated, particularly the effect of ice crystallization kinetics when supercooled liquid water changes into ice. The factors affecting the different operating conditions and structural features of the membrane electrode assembly (MEA) are investigated. The results show that when the start temperature is -20 °C or higher, ice formation is delayed and the formation rate is decreased, and supercooled liquid water permeates from the CL into the MPL. For an MEA with relatively high hydrophobicity, the water permeation rate is high. These results can enable a PEMFC to start at subzero temperatures. The effect of ice crystallization kinetics is negligible when the fuel cell is started at -30 °C or below.
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http://dx.doi.org/10.3390/polym14153203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370896PMC
August 2022

Synthetic MRI in differentiating benign from metastatic retropharyngeal lymph node: combination with diffusion-weighted imaging.

Eur Radiol 2022 Aug 11. Epub 2022 Aug 11.

Department of Radiology, Affiliated Hospital of Jiangnan University, 1000 Hefeng Road, Wuxi, 214122, People's Republic of China.

Objectives: This study aimed to evaluate the synthetic MRI (syMRI), its combination with diffusion-weighted imaging (DWI), and morphological features for discriminating benign from metastatic retropharyngeal lymph nodes (RLNs).

Methods: Fifty-eight patients with a total of 63 RLNs (21 benign and 42 metastatic) were enrolled. The mean and standard deviation of syMRI-derived relaxometry parameters (T1, T2, PD; T1, T2, PD) were obtained from two different regions of interest (namely, partial-lesion and full-lesion ROI). The parameters derived from benign and metastatic RLNs were compared using Student's t or chi-square tests. Logistic regression analysis was used to construct a multi-parameter model of syMRI, syMRI + DWI, and syMRI + DWI + morphological features. Areas under the curve (AUC) were compared using the DeLong test to determine the best diagnostic approach.

Results: Benign RLNs had significantly higher T1, T2, PD, and T1 values compared with metastatic RLNs in both partial-lesion and full-lesion ROI (all p < 0.05). The T1 obtained from full-lesion ROI showed the best diagnostic performance among all syMRI-derived single parameters. The AUC of combined syMRI multiple parameters (T1, T2, PD, T1) were higher than those of any single parameter from syMRI. The combination of synthetic MRI and DWI can improve the AUC regardless of ROI delineation. Furthermore, the combination of synthetic MRI, DWI-derived quantitative parameters, and morphological features can significantly improve the overall diagnostic performance.

Conclusions: The value of syMRI has been validated in differential diagnosis of benign and metastatic RLNs, and syMRI + DWI + morphological features can further improve the diagnostic efficiency for discriminating these two entities.

Key Points: • Synthetic MRI was useful in differential diagnosis of benign and metastatic RLNs. • The combination of syMRI, DWI, and morphological features can significantly improve the diagnostic efficiency.
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http://dx.doi.org/10.1007/s00330-022-09027-4DOI Listing
August 2022

Shear characteristics and shear strength model of rock mass structural planes.

Sci Rep 2022 Aug 10;12(1):13637. Epub 2022 Aug 10.

Badong National Observation and Research Station of Geohazards, China University of Geosciences, Wuhan, 430074, China.

Accurately determining the shear strength of structural planes is crucial for evaluating the stability of rock masses. The shear test using the sawtooth structural plane usually captures the main influencing factors of its shear characteristics. In this study, the two-dimensional particle flow code (PFC2D) numerical simulation method was used to conduct shear tests on the sawtooth structural planes of rock masses with undulant angles of 10°, 20°, and 30°, respectively. With the increase in normal stress and the undulant angle, the shear failure of the structural planes was found to no longer be pure slip failure or shear failure but accompanied by a compression-induced fracture phenomenon. Based on the analysis of the shear test results, a peak shear strength model considering different undulant angles and normal stresses was proposed, and the hyperbolic function post-peak shear strength model was improved. The peak shear strength obtained from the physical direct shear tests was compared with those calculated using the proposed model, Parton model, and Shen model. The calculation error under low and high normal stress of the proposed method was found to be within an acceptable range. Additionally, when calculating the peak shear strength of a structural plane under high normal stress, applying the calculation method proposed in this study is a better option than applying the other models. Furthermore, although the variation trend of the post-peak shear strength was similar to that of the experimental results, the values obtained using the hyperbolic variation model were too large. The variation trend of the post-peak shear strength obtained using the improved function was essentially consistent with the experimental results, and the calculated values were close to the experimental results. The systematic research on the shear strength calculation model of rock mass structural planes contributes to the theoretical research of rock mass mechanics, and this study can act as a guide for landslide prediction and control projects.
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http://dx.doi.org/10.1038/s41598-022-17998-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365770PMC
August 2022

Licochalcone B induces DNA damage, cell cycle arrest, apoptosis, and enhances TRAIL sensitivity in hepatocellular carcinoma cells.

Chem Biol Interact 2022 Aug 7:110076. Epub 2022 Aug 7.

School of Pharmacy, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271000, PR China. Electronic address:

Hepatocellular carcinoma (HCC) is a highly fatal disease recognized as a growing global health crisis. Traditional Chinese herbal medicines have been used to treat patients with cancer for many years in China. This study investigated the effects of licochalcone B (LCB), a flavonoid compound isolated from the root of Glycyrrhiza uralensis Fisch., on cell proliferation, DNA damage and TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in HCC cells. Our results showed that LCB inhibited cell proliferation and induced DNA damage, cell cycle arrest and apoptosis. Treatment with LCB significantly inhibited the Akt/mTOR pathway and activated endoplasmic reticulum (ER) stress and mitogen-activated protein kinase (MAPK) signaling pathway. Moreover, combined treatment with LCB and TRAIL yielded evident enhancements in the viability reduction and apoptosis. LCB upregulated death receptor 4 (DR4) and death receptor 5 (DR5) protein in a concentration- and time-dependent manner. The knockdown of DR5 significantly suppressed TRAIL-induced cleavage of PARP, which was enhanced by LCB. Treatment with an extracellular-regulated kinase (ERK) inhibitor (PD98059) or c-Jun N-terminal kinase (JNK) inhibitor (SP600125) markedly reduced the LCB-induced upregulation of DR5 expression and attenuated LCB-mediated TRAIL sensitization. In summary, LCB exhibits cytotoxic activity through modulation of the Akt/mTOR, ER stress and MAPK pathways in HCC cells and effectively enhances TRAIL sensitivity through the upregulation of DR5 expression in ERK- and JNK-dependent manner. Combination therapy with LCB and TRAIL may be an alternative treatment strategy for HCC.
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http://dx.doi.org/10.1016/j.cbi.2022.110076DOI Listing
August 2022

Pre-operative MRI Presentations of Herlyn-Werner-Wunderlich Syndrome.

Congenit Anom (Kyoto) 2022 Aug 8. Epub 2022 Aug 8.

Department of Radiology, Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, National Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, China.

Herlyn-Werner-Wunderlich (HWW) syndrome is a rare complex female urogenital anomaly, with diverse anatomical presentations. Due to obstruction, most patients with HWW syndrome need to be addressed surgically. The treatment strategy should be tailored to the different anatomical variants of each patient. Therefore, detailed and comprehensive pre-operative evaluation is needed. In this review, we describe the embryology and clinical manifestations of HWW syndrome, and discuss and illustrate its diverse pre-operative MRI presentations to guide clinical treatment.
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http://dx.doi.org/10.1111/cga.12489DOI Listing
August 2022

Metabonomic analysis of abnormal sphingolipid metabolism in rheumatoid arthritis synovial fibroblasts in hypoxia microenvironment and intervention of geniposide.

Front Pharmacol 2022 22;13:969408. Epub 2022 Jul 22.

Key Laboratory of Xin'an Medicine, Ministry of Education, Hefei, China.

Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by a joint hypoxia microenvironment. Our previous untargeted metabolomics study found that sphingolipid (SPL) metabolism was abnormal in the joint synovial fluid samples from adjuvant arthritis (AA) rats. Geniposide (GE), an iridoid glycoside component of the dried fruit of Ellis, is commonly used for RA treatment in many Asian countries. At present, the mechanism of GE in the treatment of RA, especially in the joint hypoxia microenvironment, is not entirely clear from the perspective of SPL metabolism. The purpose of this research was to explore the potential mechanism of abnormal SPL metabolism in RA joint hypoxia microenvironment and the intervention effect of GE, through the untargeted metabolic analysis based on the ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Arthritis index, foot swelling and histopathology were used to assess whether the AA rat model was successfully established. The SPLs extracts collected from AA rats' synovial tissue, serum and rheumatoid arthritis synovial fibroblasts (RASFs, MH7A cells, hypoxia/normoxia culture) were analyzed by metabolomics and lipdomics approach based on UPLC-Q-TOF/MS, to identify potential biomarkers associated with disorders of GE regulated RA sphingolipid metabolism. As a result, 11 sphingolipid metabolites related to RA were screened and identified. Except for galactosylceramide (d18:1/20:0), GE could recover the change levels of the above 10 sphingolipid biomarkers in varying degrees. Western blotting results showed that the changes in ceramide (Cer) level regulated by GE were related to the down-regulation of acid-sphingomyelinase (A-SMase) expression in synovial tissue of AA rats. To sum up, this research examined the mechanism of GE in the treatment of RA from the perspective of SPL metabolism and provided a new strategy for the screening of biomarkers for clinical diagnosis of RA.
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http://dx.doi.org/10.3389/fphar.2022.969408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353937PMC
July 2022

Occurrence, distribution, and ecological risk of psychoactive substances in typical lakes and rivers in Qinghai-Tibet Plateau.

Ecotoxicol Environ Saf 2022 Aug 1;242:113928. Epub 2022 Aug 1.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Center for Environmental Health Risk Assessment and Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; State Environmental Protection Key Laboratory of Ecological Effect and Risk Assessment of Chemicals, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address:

The occurrence, distribution, and ecological risks of psychoactive substances (PSs) in Qinghai-Tibet Plateau (QTP) was investigated in this study. The surface water samples were collected in July in 2020 from five major water bodies, and 9 PSs were determined by liquid chromatography-mass spectrometry. The mean concentrations of the total PSs were 2.19-96.86 ng/L in lakes and 4.56-34.47 ng/L in rivers. Amphetamine (AMP) was the predominant contaminant both in lakes and rivers with a mean concentration of 12.21 ± 22.76 ng/L and 9.83 ± 6.14 ng/L, respectively. The compositions of PSs in lakes and rivers were significantly different. AMP, methadone (MTD), 3,4-methylenedioxyamphetamine (MDA), and ketamine (KET) were the most detected contaminants in lakes, while in rivers AMP, MDA, heroin (HER), and methamphetamine (METH) were the most detected ones. Concentrations of AMP and MTD, the two predominant drugs, varied spatially, with the decreasing concentration of AMP in the order of Huangshui River > Yamzhog Yumco Lake > Qinghai Lake > Lhasa River > Namco Lake, and of MTD in the order of Qinghai Lake > Namco Lake > Huangshui River > Yamzhog Yumco Lake. The risk quotients (RQs) of PSs ranged from 4.44 × 10 to 4.32 × 10, indicating a low risk of PSs in the aquatic ecosystem in QTP. Compared with other research in the world, the contamination of psychoactive substances in the Qinghai-Tibet Plateau was at relatively low levels with low ecological risks.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113928DOI Listing
August 2022

Adsorption behavior and mechanism of amine/quaternary ammonium lignin on tungsten.

Int J Biol Macromol 2022 Sep 1;216:882-890. Epub 2022 Aug 1.

The State Key Laboratory of Refractories and Metallurgy, Wuhan University of Science and Technology, Wuhan, Hubei 430081, China; Key Laboratory for Ferrous Metallurgy and Resources Utilization of Ministry of Education, Wuhan University of Science and Technology, Wuhan, Hubei 430081, China.

Amine/quaternary ammonium lignin for adsorption of tungsten was synthesized by amination and quaternization from lignin. The adsorbent was characterized by SEM-EDS and FTIR. The effects of pH, initial concentration of tungsten, adsorption time and dosage of adsorbent on the adsorption effect were investigated. The adsorption mechanism was revealed by SEM-EDS and FTIR and XPS. The results showed that amine/quaternary ammonium lignin was loose and rough and contained a large number of phenolic hydroxyl and amine and quaternary ammonium functional groups. Using the optimum conditions, which included the pH of 4.0 and initial tungsten concentration of 800 mg·L and adsorption time of 960 min, the saturated adsorption capacity of 1 g·L amine/quaternary ammonium lignin for tungsten reached 421.68 mg·g. The adsorption followed Langmuir model and quasi-second-order kinetic model, indicating that the adsorption was monolayer homogeneous chemisorption. When the total concentration of tungsten was 0.005 mol·L and the value of pH was smaller than 4.7, HWO was the existing form of tungsten and was adsorbed by electrostatic attraction of hydrogen bond and coordination with amino and ion exchange with Cl.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.226DOI Listing
September 2022

Re: the association between histological prostatitis and benign prostatic hyperplasia: a single-center retrospective study.

Aging Male 2022 12;25(1):192

Department of Urology, Guizhou Provincial People's Hospital, Guiyang, China.

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http://dx.doi.org/10.1080/13685538.2022.2099829DOI Listing
December 2022

m6A methyltransferase METTL3-induced lncRNA SNHG17 promotes lung adenocarcinoma gefitinib resistance by epigenetically repressing LATS2 expression.

Cell Death Dis 2022 Jul 28;13(7):657. Epub 2022 Jul 28.

Department of General Thoracic Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, P. R. China.

Gefitinib has been widely applied for the treatment of lung adenocarcinoma (LUAD). However, the long-term application of gefitinib usually leads to acquired drug resistance in tumour patients, resulting in clinical treatment failure. Small nucleolar host gene 17 (SNHG17) has been shown to play a regulatory role in LUAD progression. Nevertheless, the role of SNHG17 in LUAD gefitinib resistance remains elusive. The expression pattern of SNHG17 was examined in tissues and cell lines of gefitinib-sensitive and gefitinib-resistant LUAD, respectively. Gain- and loss-of-function experiments were employed to assess the biological functions of SNHG17 in cell proliferation and apoptosis, as well as aggressive phenotypes of LUAD cells. MeRIP-qPCR and colorimetric quantificational analysis were performed to detect m6A modifications and contents. Fluorescence in situ hybridisation (FISH) and subcellular fractionation analysis were used to reveal the distribution of SNHG17. RIP and ChIP assays were performed to further validate the SNHG17/EZH2/LATS2 regulatory axis. A xenograft tumour growth assay was conducted to evaluate the role of SNHG17 in LUAD gefitinib resistance in vivo. SNHG17 was upregulated in gefitinib-resistant LUAD tissues and cell lines. Functional assays showed that SNHG17 aggravated the malignant phenotypes of gefitinib-resistant LUAD cells. In addition, METTL3-mediated N-methyladenosine modification could induce the upregulation of SNHG17by stabilising its RNA transcript. Mechanistically, SNHG17 epigenetically repressed the expression of LATS2 by recruiting EZH2 to the promoter region of LATS2. The regulatory role of the SNHG17/EZH2/LATS2 axis in LUAD gefitinib resistance was further supported in vivo. Collectively, our findings suggested that SNHG17 induced by METTL3 could promote LUAD gefitinib resistance by epigenetically repressing LATS2 expression.
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http://dx.doi.org/10.1038/s41419-022-05050-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334586PMC
July 2022

Chemical and Structural Elucidation of Lignin and Cellulose Isolated Using DES from Bagasse Based on Alkaline and Hydrothermal Pretreatment.

Polymers (Basel) 2022 Jul 6;14(14). Epub 2022 Jul 6.

College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 260412, China.

The separation of cellulose, hemicellulose, and lignin components using deep eutectic solvent, which is a green solvent, to obtain corresponding chemicals can realize the effective separation and high-value utilization of these components at low cost. In this study, we used waste biomass sugarcane bagasse as the raw material, choline chloride as the hydrogen bond acceptor, and lactic acid as the hydrogen bond donor to synthesize a deep eutectic solvent of choline chloride/lactic acid (L-DES) and treated sugarcane bagasse pretreated by alkali or hydrothermal methods to separate cellulose, hemicellulose, and lignin. In addition, we comparatively studied the effect of different pretreatment methods on lignin removal by DES and found that the lignin removal rate by L-DES after alkaline pretreatment was significantly higher than that after hydrothermal pretreatment, and the mechanism of action causing this difference is discussed.
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http://dx.doi.org/10.3390/polym14142756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325185PMC
July 2022

Pd-Modified LaFeO as a High-Efficiency Gas-Sensing Material for HS Gas Detection.

Nanomaterials (Basel) 2022 Jul 18;12(14). Epub 2022 Jul 18.

Institute of Life Sciences & Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou 325035, China.

As a typical -type semiconductor gas-sensing material, LaFeO has good response stability to HS, but its responsiveness is low, and the detection limit is not low enough for large-scale use in the field of gas sensors. To obtain better performance, we synthesized Pd modified LaFeO using the sol-gel method. A total of 3 wt% of Pd-LaFeO with a high specific surface area had the highest response to HS (36.29-1 ppm) at 120 °C, with relatively fast response-recovery times (19.62/15.22 s), and it had higher selectivity to HS with other gases. Finally, we detected the HS concentrations in the air around the shrimps, and the HS concentrations that we obtained by the 3 wt% Pd-LaFeO in this study were within 10% of those obtained by GC-MS. According to the experimental results, noble-metal surface modification improves the performance of gas-sensing materials, and Pd-LaFeO has considerable potential in HS detection.
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http://dx.doi.org/10.3390/nano12142460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316696PMC
July 2022

Mitochondrial Genome of (Plecoptera: Taeniopterygidae), with a Phylogenetic Analysis of Nemouroidea.

Genes (Basel) 2022 Jun 22;13(7). Epub 2022 Jun 22.

Henan International Joint Laboratory of Taxonomy and Systematic Evolution of Insecta, Henan Institute of Science and Technology, Xinxiang 453003, China.

Taeniopterygidae is a medium-sized family of stoneflies. The phylogeny of Taeniopterygidae was widely accepted based on the morphological analyses. However, there are different opinions based on molecular data. To date, only two taeniopterygid mitochondrial genomes (mitogenomes) were available, and more sampling is needed to obtain precise phylogenetic relationships. In this research, the mitogenome was sequenced and analyzed. The complete mitogenome of was 15,527 bp in length and contained 37 genes and a non-coding control region. Among taeniopterygid mitogenomes, the length variation was minimal in protein-coding genes (PCGs), transfer RNA genes (tRNAs) and ribosomal RNA genes (rRNAs), but very different in the control region. Similar to mitogenomes of other taeniopterygid species, the mitogenome was consistently AT biased and displayed positive AT- and negative GC-skews of the whole mitogenome. Most PCGs used ATN as the start codon and TAA/TAG as the stop codon. The stop codons were far less variable than the start codons in taeniopterygid mitogenomes. All / ratios were less than 1, indicating the presence of purifying selection in these genes. The secondary structures of transfer and ribosomal RNA genes of mitogenome are highly conserved with other taeniopterygid species. In the control region of the mitogenome, some essential elements (tandem repeats, stem-loop structures, and poly-N stretch, etc.) were observed. Two phylogenetic trees were inferred from Bayesian inference (BI) and Maximum Likelihood (ML) methods generated the identical topology across the PCGR dataset. The relationships of five families in Nemouroidea were recovered as Leuctridae + ((Capniidae + Taeniopterygidae) + (Nemouridae + Notonemouridae)). These results will help us understand the mitogenome structure of taeniopterygid species and the evolutionary relationship within Plecoptera.
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http://dx.doi.org/10.3390/genes13071116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322138PMC
June 2022

A Preliminary and Evaluation of the Effect and Action Mechanism of 17-AAG Combined With Azoles Against Azole-Resistant spp.

Front Microbiol 2022 7;13:825745. Epub 2022 Jul 7.

Department of Dermatology, Jingzhou Hospital, Yangtze University, Candidate Branch of National Clinical Research Center for Skin and Immune Diseases, Jingzhou, China.

Invasive candidiasis is the primary reason for the increased cases of mortality in a medical environment. The resistance spectra of species to antifungal drugs have gradually expanded. Particularly, the resistance spectra of are the most prominent. Hsp90 plays a protective role in the stress response of fungi and facilitates their virulence. In contrast, Hsp90 inhibitors can improve the resistance of fungi to antifungal drugs by regulating the heat resistance of Hsp90, which destroys the integrity of the fungal cell walls. Hsp90 inhibitors thus offer a great potential to reduce or address fungal drug resistance. The drugs tested for the resistance include itraconazole, voriconazole, posaconazole, fluconazole, and 17-AAG. A total of 20 clinical strains of were investigated. The broth microdilution checkerboard technique, as adapted from the CLSI M27-A4 method, was applied in this study. We found that 17-AAG alone exerted limited antifungal activity against all tested strains. The MIC range of 17-AAG was 8 to >32 μg/ml. A synergy was observed among 17-AAG and itraconazole, voriconazole, and posaconazole against 10 (50%), 7 (35%), and 13 (65%) of all isolates, respectively. Moreover, the synergy between 17-AAG and fluconazole was observed against 5 (50%) strains of azole-resistant . However, no antagonism was recorded overall. Our result adequately verifies the influence of 17-AAG on the formation of spp. biofilm. Moreover, we determined that with the use of rhodamine 6G to detect drug efflux and that of dihydrorhodamine-123 to detect intracellular reactive oxygen species (ROS), treatment with 17-AAG combined with azole drugs could inhibit the efflux pump of fungi and promote the accumulation of ROS in the fungal cells, thereby inducing fungal cell apoptosis. Thus, the mechanism of 17-AAG combined with azoles can kill fungi. Our results thus provide a new idea to further explore drugs against drug-resistant spp.
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http://dx.doi.org/10.3389/fmicb.2022.825745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300965PMC
July 2022

Genome-Wide Analysis of and Gene Families in and Their Roles in Salt Stress Response.

Front Plant Sci 2022 7;13:918594. Epub 2022 Jul 7.

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

is a halophyte with exceptional nutritional qualities, and therefore it is potentially an ideal crop to grow in saline soils, not only addressing the problem of land salinization, but also providing nutrient food for the health of humans. Currently, the molecular mechanisms underlying salt tolerance in quinoa are still largely unknown. In , receptor-like kinase (RLK1Ls) FERONIA (FER) and its ligands rapid alkalinization factors (RALFs) have been reported that participate in the regulation of salt tolerance. Here, we performed a genome-wide analysis and identified 26 and 18 family genes in quinoa genome. Transcriptomic profiling of the leaf, root, stamen, and pistil tissues of quinoa reveals that different and genes exhibit tissue-specific expression patterns, which is consistent with that observed in other plant species. RNA-seq data show that three genes are highly up-regulated after salt treatment, suggesting that some family genes are transcriptionally responsive to salt stress in quinoa. Biochemical study indicates that CqRALF15, a paralog of Arabidopsis RALF22, is physically associated with RLK1L proteins CqFER and AtFER. CqRALF15 and AtRALF22 are functionally conserved in inducing the internalization of AtFER and in triggering root growth inhibition in both quinoa and Arabidopsis. Moreover, overexpression of in Arabidopsis results in enhanced leaf bleaching under salt stress, indicating that is involved in salt stress response. Together, our study characterizes and family genes in quinoa at genomic, transcriptional, and protein levels, and provides evidence to support their roles in salt stress response.
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http://dx.doi.org/10.3389/fpls.2022.918594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302450PMC
July 2022

Conditional empirical wavelet transform with modified ratio of cyclic content for bearing fault diagnosis.

ISA Trans 2022 Jun 25. Epub 2022 Jun 25.

College of Electrical Engineering, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address:

Empirical wavelet transform (EWT) is usually employed to segment Fourier spectrum for fault diagnosis. However, the original empirical segmentation approach may be easily affected by noise. In this paper, several conditions and a modified ratio of cyclic content are then proposed to help establish proper spectrum segments and to improve fault diagnosis. The proposed conditions include a pre-whitening process to reduce discrete frequency noise, a threshold to avoid white frequency noise, an additional boundary for the last considered maximum, distance requirement for consecutive local maxima, as well as one iteration of finding local extremums. Finally, the proposed method is compared with EWT and fast kurtogram methods in three case studies. The results indicate that the proposed method can provide more favorable diagnosis results.
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http://dx.doi.org/10.1016/j.isatra.2022.06.027DOI Listing
June 2022

Diagnosis of urogenital cancer combining deep learning algorithms and surface-enhanced Raman spectroscopy based on small extracellular vesicles.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Nov 7;281:121603. Epub 2022 Jul 7.

Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China. Electronic address:

Purpose: To identify and compare the capacities of serum and serum-derived small extracellular vesicles (EV) in diagnosis of common urogenital cancer combining Surface-enhanced Raman spectroscopy (SERS) and Convolutional Neural Networks (CNN).

Materials And Methods: We collected serum samples from 32 patients with prostate cancer (PCa), 33 patients with renal cell cancer (RCC) and 30 patients with bladder cancer (BCa) as well as 35 healthy control (HC), which were thereafter used to enrich extracellular vesicles by ultracentrifuge. Label-free SERS was utilized to collect Raman spectra from serum and matched EV samples. We constructed CNN models to process SERS data for classification of malignant patients and healthy controls (HCs).

Results: We collected 650 and 1206 spectra from serum and serum-derived EV, respectively. CNN models of EV spectra revealed high testing accuracies of 79.3%, 78.7% and 74.2% in diagnosis of PCa, RCC and BCa, respectively. In comparison, serum SERS-based CNN model had testing accuracies of 73.0%, 71.1%, 69.2% in PCa, RCC and BCa, respectively. Moreover, CNN models based on EV SERS data show significantly higher diagnostic capacities than matched serum CNN models with the area under curve (AUC) of 0.80, 0.88 and 0.74 in diagnosis of PCa, RCC and BCa, respectively.

Conclusion: Deep learning-based SERS analysis of EV has great potentials in diagnosis of urologic cancer outperforming serum SERS analysis, providing a novel tool in cancer screening.
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http://dx.doi.org/10.1016/j.saa.2022.121603DOI Listing
November 2022

Electrophotochemical Ce-Catalyzed Ring-Opening Functionalization of Cycloalkanols under Redox-Neutral Conditions: Scope and Mechanism.

J Am Chem Soc 2022 Aug 21;144(30):13895-13902. Epub 2022 Jul 21.

The Institute for Advanced Studies (IAS), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei, P. R. China.

Selective cleavage and functionalization of C-C bonds in alcohols is gaining increasing interest in organic synthesis and biomass conversion. In particular, the development of redox-neutral catalytic methods with cheap catalysts and clean energy is of utmost interest. In this work, we report a versatile redox-neutral method for the ring-opening functionalization of cycloalkanols by electrophotochemical (EPC) cerium (Ce) catalysis. The EPC-Ce-enabled catalysis allows for cycloalkanols with different ring sizes to be cleaved while tolerating a broad range of functional groups. Notably, in the presence of chloride as a counteranion and electrolyte, this protocol selectively leads to the formation of C-CN, C-C, C-S, or C-oxime bonds instead of a C-halide bond after β-scission. A preliminary mechanistic investigation indicates that the redox-active Ce catalyst can be tuned by electro-oxidation and photo-reduction, thus avoiding the use of an external oxidant. Spectroscopic characterizations (cyclic voltammetry, UV-vis, electron paramagnetic resonance, and X-ray absorption fine structure) suggest a Ce(III)/Ce(IV) catalytic pathway for this transformation, in which a Ce(IV)-alkoxide is involved.
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http://dx.doi.org/10.1021/jacs.2c05520DOI Listing
August 2022

Association between gestational arsenic exposure and intrauterine growth restriction: the role of folate content.

Environ Sci Pollut Res Int 2022 Jul 20. Epub 2022 Jul 20.

Department of Toxicology, Anhui Medical University, Hefei, 230032, Anhui Province, China.

Gestational arsenic (As) exposure is associated with intrauterine growth restriction (IUGR). This study explored the association among gestational As exposure, IUGR, and reduction of folate content in maternal and umbilical plasma from 530 mother-and-singleton-offspring pairs. Birth weight (BW) was negatively correlated with As in maternal plasma (r=-0.194, P<0.001) and umbilical plasma (r=-0.235, P<0.001). By contrast, a positive correlation was found between BW and maternal folate content (r=0.198, P<0.001). The subjects were divided into As-L and As-H groups. The influence of As-H on small for gestational age (SGA) infants, a marker of IUGR, was evaluated by multivariate logistic regression that excludes interferences of gestational age, infant sex, and other confounding factors. Mothers with As-H had an elevated risk of SGA infants (adjusted OR, 2.370; P<0.05). Interestingly, maternal folate content was lower in subjects with As-H than those with As-L (22.4±10.7 vs 11.2±6.7 nmol/L, P<0.001). Linear correlation models show that As level was negatively correlated with folate content in maternal plasma (r=-0.615, P<0.001) and umbilical plasma (r=-0.209, P<0.001). Moreover, maternal folate reduction has an obvious mediating effect between increased As and decreased BW (β=-0.078, P<0.05). Our results indicate that folate reduction may be a mediator between gestational As exposure and IUGR.
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http://dx.doi.org/10.1007/s11356-022-21961-wDOI Listing
July 2022

External auditory canal and middle ear tumors: characterization by morphology and diffusion features on CT and MRI.

Eur Arch Otorhinolaryngol 2022 Jul 17. Epub 2022 Jul 17.

Department of Radiology, Affiliated Hospital of Jiangnan University, 1000 Hefeng Road, Wuxi, 214122, People's Republic of China.

Purpose: To explore the value of morphology and diffusion features on CT and MRI in the characterization of external auditory canal and middle ear tumors (EAMETs).

Methods: Forty-seven patients with histologically proved EAMETs (23 benign and 24 malignant) who underwent CT and MRI were retrospectively analyzed in this study. CT and MRI characteristics (including size, shape, signal intensity, border, enhancement degree, and bone changes) and apparent diffusion coefficient (ADC) value were analyzed and compared between benign and malignant EAMETs. Logistic regression, receiver operating characteristic (ROC) curve, and Delong test were performed to assess the diagnostic performance.

Results: Compared with benign tumors, the malignant EAMETs are characterized by irregular shape, ill-defined border, invasive bone destruction, and intense enhancement (all p < 0.05). There were no significant differences on the size and signal intensity between benign and malignant tumors. The ADC value of malignant tumors were (879.96 ± 201.15) × 10 mm/s, which was significantly lower than benign ones (p < 0.05). Logistic regression demonstrates the presence of ill-defined margin, invasive bone destruction, and low ADC value (≤ 920.33 × 10 mm/s) have significant relationship with malignant EAMETs. The combination of characterization by morphology and diffusion features on CT and MRI can further improve the diagnostic efficiency when compared with morphology and diffusion features alone (both p < 0.05).

Conclusion: Some CT and MRI characteristics are helpful in identifying malignant EAMETs from benign ones (especially ill-defined margin, invasive bone destruction, and low ADC value), and the combination of morphology and diffusion features on CT and MRI has best diagnostic efficiency for discriminating these two entities.
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http://dx.doi.org/10.1007/s00405-022-07509-1DOI Listing
July 2022

Effects of secukinumab and adalimumab on serum uric acid level in patients with plaque psoriasis.

Chin Med J (Engl) 2022 Jul 18. Epub 2022 Jul 18.

Department of Dermatology, Peking University People's Hospital, Beijing 100044, China.

Background: Psoriasis is a chronic systemic inflammatory disease, and hyperuricemia is a common comorbidity in patients with psoriasis. However, there are limited reports on the relationship between serum uric acid levels and biological treatment efficacy. The purposes of this study were to compare the differences in serum uric acid levels between patients with psoriasis and healthy controls and analyze the risk of hyperuricemia.

Methods: A total of 196 patients with psoriasis and 191 age- and sex-matched healthy controls were enrolled in this retrospective cohort study. One hundred and twenty-seven patients with severe psoriasis were treated with biologics. Sixty-eight patients received adalimumab, and 59 patients received secukinumab. Serum uric acid levels were measured at baseline, week 24, and week 48of treatment.

Results: Patients with psoriasis had higher serum uric acid levels than healthy controls (6.4 ± 1.7 mg/dL vs. 5.7 ± 1.5 mg/dL, P < 0.001). Hyperuricemia was found in 33.7% (66/196) of (66/196) patients with psoriasis, which was significantly higher than that in healthy controls (13.1% [25/191] [25/191], P < 0.001). Serum uric acid levels and hyperuricemia were not related to the severity of psoriasis (P > 0.05). No significant changes in serum uric acid levels and hyperuricemia were observed following adalimumab treatment (P > 0.05). The serum uric acid level in patients treated with secukinumab was 6.7 ± 1.6 mg/dL at week 24, which was not statistically different from that at baseline (6.6 ± 1.4 mg/dL, P = 0.885). Serum uric acid levels were significantly decreased at week 48 (6.3 ± 1.5 mg/dL vs. 6.6 ± 1.4 mg/dL, P = 0.007) in patients treated with secukinumab. Secukinumab had no significant effect on hyperuricemia either (P > 0.05).

Conclusions: The serum uric acid levels and prevalence of hyperuricemia in patients with psoriasis were significantly higher than those in healthy controls. Secukinumab treatment for 48 weeks successfully decreased serum uric acid levels in patients with psoriasis, whereas adalimumab had no significant effect on serum uric acid levels.
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http://dx.doi.org/10.1097/CM9.0000000000002130DOI Listing
July 2022

Effects of adalimumab and secukinumab on comorbidities associated with metabolism in patients with plaque psoriasis.

Chin Med J (Engl) 2022 Jul 18. Epub 2022 Jul 18.

Department of Dermatology, Peking University People's Hospital, Beijing 100044, China.

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http://dx.doi.org/10.1097/CM9.0000000000002143DOI Listing
July 2022

Synthesis of Cyclopentene Derivatives via Electrochemically Induced Intermolecular Selective (3+2) Annulation.

Angew Chem Int Ed Engl 2022 Jul 11:e202207059. Epub 2022 Jul 11.

The Institute for Advanced Studies (IAS), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, P. R. China.

Cyclopentenes are common cores in many natural products, and in bioactive and functional molecules. However, their synthesis remains challenging in terms of harsh conditions, poor selectivity, prefunctionalization of the substrates, over-reliance on volatile activating reagents and the use of noble metals. Herein, we develop an electrochemical mediator-induced intermolecular selective (3+2) annulation of readily available alkenes and alkynes/alkenes, which provides a simple and efficient method for the synthesis of a library of decorated cyclopentenes and cyclopentanes. This protocol features high efficiency, mild reaction conditions, broad substrate scope, good functional group tolerance, and high regioselectivity. Potential applications are demonstrated by gram-scale synthesis as well as the construction of structural diversity and complexity. A preliminary mechanistic investigation was performed, which indicated that an iodine radical and carbon radicals are involved in this transformation.
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http://dx.doi.org/10.1002/anie.202207059DOI Listing
July 2022
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