Dr Helene Martin-Yken, PhD - INRA / IRD - Academic Researcher

Dr Helene Martin-Yken

PhD

INRA / IRD

Academic Researcher

PAPEETE - TAHITI, Tahiti | French Polynesia

Main Specialties: Biology, Biotechnology, Medical Microbiology, Medical Toxicology

Additional Specialties: Mycology

ORCID logohttps://orcid.org/0000-0002-2024-3232


Top Author

Dr Helene Martin-Yken, PhD - INRA / IRD - Academic Researcher

Dr Helene Martin-Yken

PhD

Introduction

Helene Martin-Yken works for the French National Institute for Agricultural Research (INRA). Helene has been using Cell Biology, Microbiology, Molecular Biology and Biophysics to study Fungal Morphogenesis and Fungal Cell Wall Synthesis for several years in the Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP, Toulouse, France).
She is curently working the French Research Institute for Developpment (IRD) and has started to design and develop new biosensors based on yeast cells to detect marine toxins, in the Biotoxins Research Laboratory at Institut Lous Malardé in Papeete (French Polynesia).

Primary Affiliation: INRA / IRD - PAPEETE - TAHITI, Tahiti , French Polynesia

Specialties:

Additional Specialties:

Research Interests:


View Dr Helene Martin-Yken’s Resume / CV

Education

Dec 2011
Polytechnic National Institute (Toulouse)
Habilitation to Direct Research
Nov 1998
University of Toulouse
PhD
with distinction
Jun 1995
INSA Toulouse
Engineer Diploma
1 rst of Promotion

Experience

Nov 1999
Post-Doc at Johns Hopkins Hospital (Baltimore)
Performing Reasearch

Publications

24Publications

190Reads

24Profile Views

A conserved fungal hub protein involved in adhesion and drug resistance in the human pathogen Candida albicans.

The Cell Surface

Abstract :

Drug resistance and cellular adhesion are two key elements of both dissemination and prevalence of the human
fungal pathogen Candida albicans. Smi1 belongs to a family of hub proteins conserved among the fungal kingdom
whose functions in cellular signaling affect morphogenesis, cell wall synthesis and stress resistance. The data
presented here indicate that C. albicans SMI1 is a functional homolog of Saccharomyces cerevisiae KNR4 and is
involved in the regulation of cell wall synthesis. Expression of SMI1 in S. cerevisiae knr4Δ null mutants rescued
their sensitivity to caspofungin and to heat stress. Deletion of SMI1 in C. albicans resulted in sensitivity to the
cell-wall-perturbing compounds Calcofluor White and Caspofungin. Analysis of wild-type and mutant cells by
Atomic Force Microscopy showed that the Young’s Modulus (stiffness) of the cell wall was reduced by 85% upon
deletion of SMI1, while cell surface adhesion measured by Force Spectroscopy showed that the surface expression
of adhesive molecules was also reduced in the mutant. Over-expression of SMI1, on the contrary, increased
cell surface adhesion by 6-fold vs the control strain. Finally, Smi1-GFP localized as cytoplasmic patches
and concentrated spots at the sites of new cell wall synthesis including the tips of growing hyphae, consistent
with a role in cell wall regulation. Thus, Smi1 function appears to be conserved across fungi, including the yeast
S. cerevisiae, the yeast and hyphal forms of C. albicans and the filamentous fungus Neurospora crassa.

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December 2018
1 Read

The Role of Glycans in Bacterial Adhesion to Mucosal Surfaces: How Can Single-Molecule Techniques Advance Our Understanding?

Microorganisms 2018 May 4;6(2). Epub 2018 May 4.

Department of Biotechnology and Food Science, NTNU the Norwegian University of Science and Technology, NO-7491 Trondheim, Norway.

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http://dx.doi.org/10.3390/microorganisms6020039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027152PMC
May 2018
3 Reads

Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool?

Environ Res 2018 04 4;162:144-151. Epub 2018 Jan 4.

Laboratoire des Micro-Algues Toxiques, Institut Louis Malardé, UMR 241-EIO, BP 30 98713 Papeete, Tahiti, Polynésie Française.

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http://dx.doi.org/10.1016/j.envres.2017.12.022DOI Listing
April 2018
5 Reads
4.373 Impact Factor

Knr4: a disordered hub protein at the heart of fungal cell wall signalling.

Cell Microbiol 2016 09 28;18(9):1217-27. Epub 2016 Jun 28.

LISBP, Université Fédérale de Toulouse, CNRS, INRA, INSA, 135 Avenue de Rangueil, F-31077, Toulouse, France.

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http://dx.doi.org/10.1111/cmi.12618DOI Listing
September 2016
3 Reads
4.915 Impact Factor

Crystallographic studies of the structured core domain of Knr4 from Saccharomyces cerevisiae.

Acta Crystallogr F Struct Biol Commun 2015 Sep 25;71(Pt 9):1120-4. Epub 2015 Aug 25.

Institut de Pharmacologie et de Biologie Structurale (IPBS), Centre National de la Recherche Scientifique (CNRS), 205 Route de Narbonne, BP 64182, 31077 Toulouse, France.

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http://dx.doi.org/10.1107/S2053230X15012522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555916PMC
September 2015
12 Reads

Cell Wall Remodeling Enzymes Modulate Fungal Cell Wall Elasticity and Osmotic Stress Resistance.

MBio 2015 Jul 28;6(4):e00986. Epub 2015 Jul 28.

School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, United Kingdom

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http://dx.doi.org/10.1128/mBio.00986-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551979PMC
July 2015
5 Reads
6.786 Impact Factor

Natural yeast promoter variants reveal epistasis in the generation of transcriptional-mediated noise and its potential benefit in stressful conditions.

Genome Biol Evol 2015 Mar 11;7(4):969-84. Epub 2015 Mar 11.

Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés, UMR CNRS 5504, UMR INRA 792, INSA/Université de Toulouse, France

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http://dx.doi.org/10.1093/gbe/evv047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4419794PMC
March 2015
3 Reads
4.229 Impact Factor

Targeted changes of the cell wall proteome influence Candida albicans ability to form single- and multi-strain biofilms.

PLoS Pathog 2014 Dec 11;10(12):e1004542. Epub 2014 Dec 11.

Institut Pasteur, Unité Biologie et Pathogénicité Fongiques, Département Génomes et Génétique, Paris, France; INRA, USC2019, Paris, France.

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http://dx.doi.org/10.1371/journal.ppat.1004542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263760PMC
December 2014
18 Reads

A combined chemical and enzymatic method to determine quantitatively the polysaccharide components in the cell wall of yeasts.

FEMS Yeast Res 2014 Sep 28;14(6):933-47. Epub 2014 Jul 28.

INSA, UPS, INP, Université de Toulouse, Toulouse, France; UMR792 Ingénierie des Systèmes Biologiques et des Procédés, INRA, Toulouse, France; UMR5504, CNRS, Toulouse, France; Lallemand SAS, Blagnac, France.

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http://dx.doi.org/10.1111/1567-1364.12182DOI Listing
September 2014
3 Reads
2.818 Impact Factor

Use of atomic force microscopy (AFM) to explore cell wall properties and response to stress in the yeast Saccharomyces cerevisiae.

Curr Genet 2013 Nov 27;59(4):187-96. Epub 2013 Sep 27.

Université de Toulouse, INSA, UPS, INP, 135 avenue de Rangueil, 31077, Toulouse, France,

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http://dx.doi.org/10.1007/s00294-013-0411-0DOI Listing
November 2013
5 Reads
2.682 Impact Factor

10th Francophone Yeast Meeting 'Levures, Modèles & Outils'.

Res Microbiol 2012 Jun 31;163(5):309-15. Epub 2012 May 31.

Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés, INSA, CNRS UMR5504, INRA UMR792, Université de Toulouse, INSA, UPS, INP, 135 avenue de Rangueil, 31077 Toulouse cedex 4, France.

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http://dx.doi.org/10.1016/j.resmic.2012.05.007DOI Listing
June 2012
12 Reads
2.705 Impact Factor

An atomic force microscopy analysis of yeast mutants defective in cell wall architecture.

Yeast 2010 Aug;27(8):673-84

CNRS UPR8001, LAAS, 7 Avenue du Colonel Roche, F-31077 Toulouse, France.

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http://dx.doi.org/10.1002/yea.1801DOI Listing
August 2010
44 Reads
1.634 Impact Factor

Knr4 N-terminal domain controls its localization and function during sexual differentiation and vegetative growth.

Yeast 2010 Aug;27(8):563-74

University of Toulouse, INSA, UPS, INP, INRA-UMR 792 and CNRS-UMR 5504, Ingénierie des Systèmes Biologiques et Procédés, 135 Avenue de Rangueil, F-31400 Toulouse, France.

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http://dx.doi.org/10.1002/yea.1804DOI Listing
August 2010
18 Reads
1.634 Impact Factor

Shear-flow induced detachment of Saccharomyces cerevisiae from stainless steel: influence of yeast and solid surface properties.

Colloids Surf B Biointerfaces 2006 May 18;49(2):126-35. Epub 2006 Apr 18.

Laboratoire de Biotechnologie-Bioprocédés UMR CNRS 5504 - UMR INRA 792, INSA, 135 avenue de Rangueil, 31 077 Toulouse cedex 4, France.

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http://linkinghub.elsevier.com/retrieve/pii/S092777650600076
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http://dx.doi.org/10.1016/j.colsurfb.2006.03.001DOI Listing
May 2006
13 Reads
4.152 Impact Factor

The 'interactome' of the Knr4/Smi1, a protein implicated in coordinating cell wall synthesis with bud emergence in Saccharomyces cerevisiae.

Mol Genet Genomics 2006 Mar 16;275(3):217-30. Epub 2005 Dec 16.

Laboratoire de Biotechnologie et Bioprocédés, UMR-CNRS 5504 & INRA 792, 135, Avenue de Rangueil, 31077 Toulouse Cedex 04, France.

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http://dx.doi.org/10.1007/s00438-005-0082-8DOI Listing
March 2006
6 Reads

The interaction of Slt2 MAP kinase with Knr4 is necessary for signalling through the cell wall integrity pathway in Saccharomyces cerevisiae.

Mol Microbiol 2003 Jul;49(1):23-35

Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, UR-INRA 792, 135, avenue de Rangueil, F-31077, Toulouse, France.

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July 2003
3 Reads
4.419 Impact Factor

Genome-wide analysis of the response to cell wall mutations in the yeast Saccharomyces cerevisiae.

J Biol Chem 2003 May 18;278(22):20345-57. Epub 2003 Mar 18.

Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504 and INRA 792, France.

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http://dx.doi.org/10.1074/jbc.M211604200DOI Listing
May 2003
1 Read
4.573 Impact Factor

KNR4 is a member of the PKC1 signalling pathway and genetically interacts with BCK2, a gene involved in cell cycle progression in Saccharomyces cerevisiae.

Curr Genet 2002 Aug 23;41(5):323-32. Epub 2002 Jul 23.

Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, UR-INRA 792, 31077 Toulouse, France.

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http://dx.doi.org/10.1007/s00294-002-0299-6DOI Listing
August 2002
1 Read
2.682 Impact Factor

Involvement of GFA1, which encodes glutamine-fructose-6-phosphate amidotransferase, in the activation of the chitin synthesis pathway in response to cell-wall defects in Saccharomyces cerevisiae.

Eur J Biochem 2002 Mar;269(6):1697-707

Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, UR-INRA 792, Département de Génie Biochimique et Alimentaire, Complexe Scientifique de Rangeuil, Toulouse, France.

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March 2002
6 Reads