Publications by authors named "Heba Roshdy"

3 Publications

  • Page 1 of 1

Monitoring of Antimicrobial Susceptibility of Bacteria Isolated from Poultry Farms from 2014 to 2018.

Vet Med Int 2021 10;2021:6739220. Epub 2021 Sep 10.

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center (ARC), Nadi El-Seid Street, Dokki P.O. Box 246, Giza 12618, Egypt.

The current situation of antibiotic resistance of most bacterial pathogens was a threat to the poultry and public health with increasing economic losses. Regarding this problem, monitoring of the circulating microorganisms occurred with the antibiotic resistance profile. A total of 657 different samples from internal organs (liver, heart, lung, and yolk) and paper-lining chick boxes were collected from native chicken farms which were submitted to the Reference Laboratory for Veterinary Quality Control on Poultry Production in the period from 2014 to 2018 for the detection of , (. , and . The bacterial isolates were tested for their antimicrobial susceptibility by disk diffusion technique. was isolated from 128 out of 657 (19.5%), . was isolated from 496 out of 657 (75.5%), and species was isolated from 497 out of 657 (75.6%). All positive samples were examined for antibiotic resistance against 10 different antibiotics, and the highest percentage all over the five years was against penicillin, ampicillin, and tetracycline. All E. positive samples were examined for antibiotic resistance against 14 different antibiotics, and the highest percentage all over the five years was with ampicillin, tetracycline, norfloxacin, streptomycin, and danofloxacin. All positive sample species were examined for antibiotic resistance against 14 different antibiotics, and the highest percentage of resistance all over the five years was shown with tetracycline, streptomycin, ampicillin, and nalidixic acid.
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http://dx.doi.org/10.1155/2021/6739220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448596PMC
September 2021

Detection of aerobic bacterial pathogens associated with early embryonic death in pregnant New Zealand female Rabbits in Egypt.

Vet World 2021 Apr 24;14(4):986-995. Epub 2021 Apr 24.

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center, Nadi El-Seid Street, Dokki P.O. Box246, Giza 12618, Egypt.

Background And Aim: Rabbits are a highly sensitive species and susceptible to various bacterial pathogens that may be causative agents for early embryonic death. This study aimed to explore the administration of different bacterial agents in does suffering from early embryonic death. Furthermore, identification of genes associated with virulence was performed to identify the phenotypic and genotypic antimicrobial resistance patterns that may increase the virulence of pathogens and lead to early embryonic death.

Materials And Methods: We isolated and identified bacterial agents in 106 samples from live and dead female rabbits that had undergone early embryonic death, including liver and intestine tissue, aborted fetuses, discharges, and vaginal swabs. Conventional polymerase chain reaction (PCR) was conducted to confirm the identity of the isolated bacterial strains and their virulence. Moreover, antibiotic resistance was studied phenotypically and genotypically.

Results: We isolated , , , , and . PCR confirmed typical identification except in , which was confirmed as spp. in some cases. The final percentage of detection was 34%, 30.2%, 16.9%, 13.2%, and 11.3%, respectively. Virulence properties were investigated using different designated genes. All strains harbored , , , and genes, while the gene was identified in 31.25%. strains harboring the gene lacked the shiga toxin () gene. and strains harbored the hemolysin gene (66.7% and 33.4%, respectively). Multidrug resistance was detected phenotypically and genotypically in most strains. Each bacterial pathogen had a different antibiotic resistance profile.

Conclusion: Multiple bacterial species may contribute to early embryonic death in does. Furthermore, the combined infection could be the main cause of early embryonic death. Thus, monitoring programs should bear this in mind and focus on the early detection of these bacterial agents in female rabbits to avoid embryonic death.
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http://dx.doi.org/10.14202/vetworld.2021.986-995DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167537PMC
April 2021

Antibiotic resistance and extended-spectrum β-lactamase in isolates from imported 1-day-old chicks, ducklings, and turkey poults.

Vet World 2020 Jun 10;13(6):1037-1044. Epub 2020 Jun 10.

Department of Bacteriology, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, P.O. Box 264, Dokki, Giza 12618, Egypt.

Aim: Antimicrobial resistance is a global health threat. This study investigated the prevalence of in imported 1-day-old chicks, ducklings, and turkey poults.

Materials And Methods: The liver, heart, lungs, and yolk sacs of 148 imported batches of 1-day-old flocks (chicks, 45; ducklings, 63; and turkey poults, 40) were bacteriologically examined for the presence of .

Results: We isolated 38 strains from 13.5%, 6.7%, and 5.4% of imported batches of 1-day-old chicks, ducklings, and turkey poults, respectively. They were serotyped as O91, O125, O145, O78, O44, O36, O169, O124, O15, O26, and untyped in the imported chicks; O91, O119, O145, O15, O169, and untyped in the imported ducklings; and O78, O28, O29, O168, O125, O158, and O115 in the imported turkey poults. The isolates were investigated for antibiotic resistance against 16 antibiotics using the disk diffusion method and were found resistant to cefotaxime (60.5%), nalidixic acid (44.7%), tetracycline (44.7%), and trimethoprim-sulfamethoxazole (42.1%). The distribution of extended-spectrum β-lactamase (ESBL) and β-lactamase genes was (52.6%), (28.9%), (39.5%), (13.1%), and (28.9%).

Conclusion: Imported 1-day-old poultry flocks may be a potential source for the dissemination of antibiotic-resistant and the ESBL genes in poultry production.
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http://dx.doi.org/10.14202/vetworld.2020.1037-1044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396329PMC
June 2020
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