Publications by authors named "He Shi"

61 Publications

Plasma EBF1 as a Novel Biomarker for Postmenopausal Osteoporosis.

J Clin Densitom 2021 Jun 16. Epub 2021 Jun 16.

The Second Ward of Department of Orthopedics, Daqing Longnan Hospital, Daqing, Heilongjiang, China. Electronic address:

Postmenopausal osteoporosis (OPO) is one of the most common types of primary osteoporosis. There is currently lack of a plasma biomarker for sensitive and early diagnosis of OPO. Here we aimed to explore the potential of early B cell factor 1 (EBF1) as a new plasma biomarker of OPO. Quantitative real-time PCR was used to measure the plasma EBF1 levels. Absorptiometry markers, such as lumbar spine (LS) bone mineral density (BMD) and LS T score were obtained after X-ray scans. Biochemical analyses used to measure osteopontin (OPN), β-isomerized C-terminal telopeptides and total N-terminal procollagen of type-I collagen levels of patients with osteopenia (OPE, n = 81), osteoporosis (OPO, n = 98) as well as healthy subjects (NC, n = 110). Quantitative real-time PCR was used to measure the plasma levels of PAX5 and GSTP1, which are target genes of EBF1. EBF1 was downregulated in OPO patients. Levels of EBF1 were positively correlated to clinicopathological characteristics, including LS BMD and LS T scores, and negatively correlated to OPN and total N-terminal procollagen of type-I collagen levels. Increased PAX5 and GSTP1 levels also demonstrated strong correlations with higher EBF1, LS BMD and LS T score. Anti-osteoporotic treatment resulted in significant upregulation of EBF1, PAX5 and GSTP1 at 6 mo after treatment. Our study suggests that plasma EBF1 is a potential biomarker for diagnosing and assessing treatment outcome of OPO.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jocd.2021.06.001DOI Listing
June 2021

Characterization of a transcription factor SlNAC7 gene from Suaeda liaotungensis and its role in stress tolerance.

J Plant Res 2021 May 8. Epub 2021 May 8.

School of Life Sciences, Liaoning Normal University, Dalian, 116081, China.

NAC (NAM, ATAF1/2, CUC2) transcription factors play important roles in plant growth, development, and responses to abiotic stress. In this study, we cloned an NAC2 subfamily transcription factor gene (SlNAC7) from the halophyte Suaeda liaotungensis K., and conducted a series of studies to determine the characteristics and functions of this gene. The SlNAC7 coding region contains 1719 base pairs that encode a 573 amino acid long protein. SlNAC7 is expressed in the roots, stems, and leaves of S. liaotungensis, with the highest expression in the leaves. We found that SlNAC7 expression can be induced by drought, salt, cold, and abscisic acid. Transient expression in onion epidermal cells revealed that SlNAC7 is located in both the nucleus and cytoplasm. A transcriptional activation experiment in yeast showed that the transcriptional activation domain of SlNAC7 is located at the C terminus. When SlNAC7 was transformed into Arabidopsis under the control of a CaMV 35S promoter its overexpression was found to enhance the ability of transgenic plants to resist drought, salt, and cold stress. Moreover, these plants showed multiple changes in growth characteristics and physiological and biochemical indices in response to different stresses, as well as the upregulation of numerous stress-related genes. We have thus characterized a new halophyte-derived NAC transcription factor, SlNAC7, which can regulate plant growth and physiological and biochemical changes under adverse conditions by regulating the expression of stress-related genes, thereby enhancing plant stress resistance. SlNAC7 is a promising candidate for breeding new varieties of stress-tolerant crops.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10265-021-01309-0DOI Listing
May 2021

An Effective Strategy for the Production of Lauric Acid-Enriched Monoacylglycerol via Enzymatic Glycerolysis from Black Soldier Fly (Hermetia illucens) Larvae (BSFL) Oil.

Appl Biochem Biotechnol 2021 Apr 19. Epub 2021 Apr 19.

School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China.

Here, we developed an efficient strategy for the production of lauric acid-enriched monoacylglycerol (MAG) via enzymatic glycerolysis using black soldier fly (Hermetia illucens) larvae (BSFL) oil. The effects of the substrate molar ratio, reaction temperature, type of immobilized lipase, and organic solvent on the MAG content and conversion degree of BSFL oil were optimized. The maximum substrate conversion rate (97.88%) and MAG content (70.84%) were obtained in a tert-butanol system at 50 °C with a glycerol/BSFL oil molar ratio of 4:1 by using immobilized MAS1 lipase as a catalyst. The MAG content in the purified product reached 97.7%, with lauric acid accounting for 50.2%. Improved oxidation stability was observed after glycerolysis. Overall, this study provides a new strategy for the preparation of lauric acid-enriched MAG from BSFL oil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-021-03565-1DOI Listing
April 2021

Factors Influencing Viral Clearance in Mild COVID-19 and Clinical Characteristics of Asymptomatic Patients.

Biomed Res Int 2021 26;2021:5909612. Epub 2021 Feb 26.

Infection Disease Department, Anqing Municipal Hospital, Anhui, China.

Background: The outbreak of coronavirus disease (COVID-19) has become a global public health emergency.

Objective: To evaluate the characteristics and outcomes of patients with COVID-19 in Anhui and to identify predictors of viral clearance.

Methods: We retrospectively analyzed the data collected from discharged patients with laboratory-confirmed SARS-CoV-2 infections. We compared clinical features between viral clearance and viral persistence, and evaluated factors associated with SARS-CoV-2 shedding using multiple linear regression.

Results: Among the 83 patients involved in the study, the median age was 43 years, while 60.2% were male, 35.4% had comorbidities, and the mortality was zero. The median time from illness onset to admission was 5 days (interquartile range (IQR), 2-7 days), and the median time from the illness onset to SARS-CoV-2 RNA detection was 16 days (IQR, 13-18 days). The factors influencing viral clearance were as follows: (1) delayed admission (beta 1.057, 95% CI 0.810-1.304; ≤ 0.001) and (2) underlying comorbidities (beta 1.907, 95% CI 0.198-3.616; = 0.029). No significant differences were observed in the length of stay ( = 0.246) and pneumonia between asymptomatic and symptomatic patients based on computed tomography (CT) ( = 0.124).

Conclusions: Delayed admission and underlying comorbidities may effectively predict SARS-CoV-2 RNA clearance. For those infected with SARS-CoV-2, even asymptomatic patients without any clinical symptoms should be traced and isolated. This practice may reduce the spread of SARS-CoV-2 and slow the COVID-19 pandemic caused by the virus. : This trial is registered with 2020-051.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5909612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936893PMC
March 2021

Development and application of a multiplex PCR system for drowning diagnosis.

Electrophoresis 2021 Jun 20;42(11):1270-1278. Epub 2021 Mar 20.

Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou Forensic Science Institute, Guangzhou, P. R. China.

In recent years, the DNA detection of drowning-related diatoms, cyanobacteria, and aeromonas has gradually attracted interest from forensic scientists. In this study, we described the validation and application of a novel multiplex PCR system. This system integrated 12 fluorescently labelled primers designed to amplify specific genes of diatoms, cyanobacteria, and aeromonas. The specificity studies demonstrated that this multiplex PCR system could detect nine species of diatom, seven species of cyanobacteria, and five species of aeromonas, all of which were drowning-related and widely distributed in various water circumstance of southern China. The sensitivity studies indicated that the limit concentration of template DNA was 0.0125 ng. Besides, this multiplex PCR system had good performance in sizing precision and stability, but it is not suitable for degraded DNA samples. The application into forensic casework showed that all the tissue samples from ten nondrowning cases showed negative results, and the positive rates of lung, liver, kidney, and water samples from 30 drowning bodies were 100, 86.7, 90, and 100%, respectively. Combined with results of diatom tests of MD-VF-Auto SEM method, this multiplex PCR system could help rule out nondrowning bodies and provide extra evidences to support drowning diagnosis, especially for those cases with few diatoms observed. It is expected that this multiplex PCR system has great potential for forensic drowning diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/elps.202000265DOI Listing
June 2021

Effect of selenium on thyroid autoimmunity and regulatory T cells in patients with Hashimoto's thyroiditis: A prospective randomized-controlled trial.

Clin Transl Sci 2021 Jul 9;14(4):1390-1402. Epub 2021 Apr 9.

Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Selenium (Se) is an essential trace element in human. Recent studies of Se supplementation on the effect of Hashimoto's thyroiditis (HT) have been reported, but the exact benefit is unclear as well as the underlying immunologic mechanism. We aimed to evaluate the clinical effect of Se supplement in patients with HT, and explore the potential mechanism against thyroid autoimmunity. A prospective, randomized-controlled study was performed in patients with HT assigned to two groups. Se-treated group (n = 43) received selenious yeast tablet (SYT) for 6 months, whereas no treatment in control group (n = 47). The primary outcome is the change of thyroid peroxidase antibody (TPOAb) or thyroglobulin antibody (TGAb). Second, thyroid function, urinary iodine, Se, Glutathione peroxidase3 (GPx3), and Selenoprotein P1 (SePP1) levels were measured during the SYT treatment. Meanwhile, regulatory T cells (Tregs) and their subsets activated Tregs (aTregs), resting Tregs, and secreting Tregs, as well as Helios and PD-1 expression on these cells were also detected. The results showed that SYT treatment significantly decreased TPOAb, TGAb, and thyroid stimulating hormone (TSH) levels, accompanied with the increased Se, GPx3, and SePP1, compared with the control group. Subgroup analysis revealed that subclinical HT may benefit more from this treatment in the decrease of TSH levels by interaction test. Moreover, the percentage of aTregs, Helios/Tregs, and Helios/aTregs were significantly higher in the Se-treated group than control. In conclusion, Se supplementation may have a beneficial effect on thyroid autoantibodies and thyroid function by increasing the antioxidant activity and upregulating the activated Treg cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/cts.12993DOI Listing
July 2021

[Progranulin (PGRN) promotes invasion and migration of mouse breast cancer 4T1 cells by promoting epithelial-mesenchymal transition of cancer cells and activating ERK1/2 pathway].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Feb;37(2):125-131

Ministry-of-Education Key Laboratory of Laboratory Medical Diagnostics, Chongqing Medical University, Chongqing 400016, China. *Corresponding author, E-mail:

Objective To investigate the effect of progranulin (PGRN) on the invasion and migration of mouse breast cancer 4T1 cells and its mechanism. Methods After treated with PGRN (1 μg/mL) for 24 hours, the invasion ability of breast cancer 4T1 cells was detected by Transwell invasion assay, the migration ability was detected by scratch test, and the epithelial cadherin (E-cadherin), vimentin mRNA expression was detected by real-time fluorescent quantitative PCR. Western blot assay was used to detect the expression of E-cadherin, vimentin, extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 (p-ERK1/2). After treated with 1 μg/mL PGRN and ERK1/2 signaling pathway inhibitor U0126 (10 μmol/L) simultaneously, the migration and invasion ability of 4T1 cells and the changes in the expression of E-cadherin, vimentin and p-ERK proteins were detected again. Results After treated with PGRN, the migration and invasion capabilities of breast cancer 4T1 cells were significantly enhanced; E-cadherin expression decreased; vimentin and p-ERK1/2 expression increased. After treated with ERK1/2 signaling pathway inhibitor, the ability of PGRN to promote breast cancer 4T1 cell migration, invasion and epithelial-mesenchymal transition (EMT) was significantly inhibited. Conclusion PGRN can promote the migration and invasion of breast cancer 4T1 cells by promoting EMT and activating the ERK1/2 pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
February 2021

Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8 T cell exclusion.

J Exp Clin Cancer Res 2021 Jan 4;40(1). Epub 2021 Jan 4.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China.

Background: Progranulin (PGRN), as a multifunctional growth factor, is overexpressed in multiple tumors, but the role of PGRN on tumor immunity is still unclear. Here, we studied the effect of PGRN on breast cancer tumor immunity and its possible molecular mechanism.

Methods: The changes of macrophage phenotypes after PGRN treatment were detected by western blot, quantitative polymerase chain reaction (PCR) and flow cytometry. Western blot was used to study the signal molecular mechanism of PGRN regulating this process. The number and localization of immune cells in Wild-type (WT) and PGRN breast cancer tissues were analyzed by immunohistochemical staining and immunofluorescence techniques. The activation and proliferation of CD8 T cells were measured by flow cytometry.

Results: After being treated with PGRN, the expressions of M2 markers and programmed death ligand 1 (PD-L1) on macrophages increased significantly. Signal transducer and activator of transcription 3 (STAT3) signaling pathway inhibitor Stattic significantly inhibited the expression of PD-L1 and M2 related markers induced by PGRN. In WT group, CD8 were co-localized with macrophages and PD-L1, but not tumor cells. The number of immune cells in PGRN breast cancer tissue increased, and their infiltration into tumor parenchyma was also enhanced. Moreover, in the co-culture system, WT peritoneal macrophages not only reduced the ratio of activated CD8 T cells but also reduced the proportion of proliferating CD8 T cells. The addition of programmed death receptor 1 (PD-1) and PD-L1 neutralizing antibodies effectively reversed this effect and restored the immune function of CD8 T cells.

Conclusion: These results demonstrate that PGRN promotes M2 polarization and PD-L1 expression by activating the STAT3 signaling pathway. Furthermore, through PD-1/PD-L1 interaction, PGRN can promote the breast tumor immune escape. Our research may provide new ideas and targets for clinical breast cancer immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13046-020-01786-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780622PMC
January 2021

The effect of the standing angle on reducing fatigue among prolonged standing workers.

Work 2021 ;68(s1):S281-S287

Faculty of Industrial Design Engineering, Delft University of Technology, The Netherlands.

Background: Many occupations require workers to stand for prolonged periods, which can cause discomfort, pain and even injures. Some supermarkets in life provide a foot pad for checkout staff to let them stand on it at work, thereby reducing standing fatigue caused by standing for a long time. The inclined platform is the same as the foot pad mentioned above. That is, the staff stepped on it and relieved standing fatigue to a certain extent.

Objective: The study aims to analyze how the standing angle affects fatigue among prolonged standing workers and tries to find an inclined platform with a specific angle to reduce standing fatigue.

Methods: This experiment studied fatigue of the inclined platforms with different angles on prolonged standing workers, eight participants were selected to participate in the test. The plantar pressures and sEMG (Surface Electromyography) were used to collect the physiological information change of prolonged standing participants in the lower limb and waist. The visual analogue scale was used as a subjective method to measure the psychological fatigue.

Results And Conclusion: The study highlights the relationship between standing angle and lower limb fatigue. The inclination of the standing platform has different effects on the participants under different time conditions. When participants stand on inclined platforms at 0°, 5° and 10°, the iEMG (Integrated Electromyography) values of the gastrointestinal muscle were not significantly different until the third sampling point (40 minutes). After that self-regulation of lower limb muscles is better when standing on an inclined platform between 5° and 10°, it has a certain effect on alleviating lower limb fatigue. This knowledge is crucial for the design of the inclined working platforms fitting the needs of prolonged standing workers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3233/WOR-208026DOI Listing
June 2021

PGRN TAMs-derived exosomes inhibit breast cancer cell invasion and migration and its mechanism exploration.

Life Sci 2021 Jan 9;264:118687. Epub 2020 Nov 9.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China. Electronic address:

Breast cancer is one of the most malignant diseases world-wide and ranks the first among female cancers. Progranulin (PGRN) plays a carcinogenic role in breast cancer, but its mechanisms are not clear. In addition, there are few reports on the relationship between PGRN and tumor-associated macrophages (TAMs).

Aims: To investigate the effects of exosomes derived from PGRN TAMs on invasion and migration of breast cancer cells.

Main Methods: Mouse breast cancer xenograft model was constructed to explore the effect of PGRN tumor environment (TME) on breast cancer. Flow cytometry was used to compare TAMs of wild type (WT) and PGRN tumor tissue. Transwell assay, wound healing assay and western blot were used to explore the effect of WT and PGRN TAMs and their exosomes on invasion, migration and epithelial-mesenchymal transition (EMT) of breast cancer cells. MicroRNA (miRNA) assay was used to find out the differentially expressed miRNA of negative control (NC) and siPGRN-TAMs exosomes. Quantitative PCR and luciferase report assay were used to explore the target gene.

Key Findings: The lung metastasis of breast cancer of PGRN mice was inhibited. PGRN TAMs inhibited invasion, migration and EMT of breast cancer cells through their exosomes. MiR-5100 of PGRN TAMs-derived exosomes was up-regulated, which might regulate expression of CXCL12, thereby inhibiting the CXCL12/CXCR4 axis, and ultimately inhibiting the invasion, migration and EMT of breast cancer cells.

Significance: Our study elucidates a new molecular mechanism of lung metastasis of breast cancer, so it may contribute to efficient prevention and therapeutic strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2020.118687DOI Listing
January 2021

The wetting characteristics of molten Ag-Cu-Au on Cu substrates: a molecular dynamics study.

Phys Chem Chem Phys 2020 Nov;22(44):25904-25917

School of Materials Science and Engineering, Harbin Institute of Technology, Harbin, 150001, China.

Ag-Cu-Au ternary alloys are promising solder materials for wire bonding. Limited experimental studies on Ag-Cu-Au materials can be found due to the high cost of gold. In this study, face-centered-cubic Cu(100), Cu(111), and Cu(110) substrates wetted by molten Ag45Cu42Au13 were investigated via molecular dynamics (MD). As demonstrated by melting simulation results, the Ag45Cu42Au13 alloy has a lower melting temperature compared to the eutectic alloy, Ag60Cu40. MD methods were also used to investigate the dissolutive characteristics of Ag45Cu42Au13/Cu wetting. Density profiles and contact angles show an increase in wettability in the Ag45Cu42Au13/Cu(100) wetting system. For molten Ag60Cu40 and Ag45Cu42Au13 the spreading behavior on Cu(100) shows a promoted tendency, which contrasts with both Cu(111) and Cu(110). Solid-liquid adhesion is indicative of the comparative spreading degrees. The contact angles and PMF analysis of wetting behaviors on rough and smooth Cu substrates illustrate that solid-liquid adhesion in Wenzel states is stronger than in Cassie wetting states.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cp03337eDOI Listing
November 2020

Automated diatom searching in the digital scanning electron microscopy images of drowning cases using the deep neural networks.

Int J Legal Med 2021 Mar 13;135(2):497-508. Epub 2020 Aug 13.

Guangzhou Forensic Science Institute & Key Laboratory of Forensic Pathology, Ministry of Public Security, Baiyun Avenue 1708, Baiyun District, Guangzhou, People's Republic of China.

Forensic diatom test has been widely accepted as a way of providing supportive evidences in the diagnosis of drowning. The current workflow is primarily based on the observation of diatoms by forensic pathologists under a microscopy, and this process can be very time-consuming. In this paper, we demonstrate a deep learning-based approach for automatically searching diatoms in scanning electron microscopic images. Cross-validation studies were performed to evaluate the influence of magnification on performance. Moreover, various training strategies were tested to improve the performance of detection. The conclusion shows that our approach can satisfy the necessary requirements to be integrated as part of an automatic forensic diatom test.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00414-020-02392-zDOI Listing
March 2021

Evaluation of L/D ratio in a water-related case for the differentiation between drowning and postmortem immersion.

Forensic Sci Int Synerg 2019 10;1:68-70. Epub 2019 Apr 10.

Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University & Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Zhongshan 2nd Road 74, Yuexiu District, 510080, Guangzhou, China.

It is a difficult task to determine the cause of death for decomposed bodies found in water. Diatom test has been shown to provide supportive evidence for the diagnosis of drowning. According to our previous studies, the ratio of diatom content in lung tissue to diatom content in drowning medium (L/D ratio) is a very useful indicator to distinguish between drowning and postmortem immersion. In this article, we presented a case of a highly decomposed body being recovered from water with no significant findings on its cause of death. We applied the microwave digestion-vacuum filtration-automated scanning electron microscopy method (MD-VF-Auto SEM) to detect diatoms in the organs and suspected drowning medium. In this case, positive results are found in diatom tests of lung tissue, liver tissue and kidney tissue, while the L/D ratio analysis suggested that the victim was more likely to suffer from postmortem immersion. Taking the result of L/R ratio and other findings into consideration, we can eliminate the possibility of drowning. With continuous study, the L/D ratio analysis would be a valuable tool in diagnosis of drowning.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsisyn.2019.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219118PMC
April 2019

Nucleus-located PDK1 regulates growth, invasion and migration of breast cancer cells.

Life Sci 2020 Jul 26;253:117722. Epub 2020 Apr 26.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China. Electronic address:

Aims: It is well known that pyruvate dehydrogenase kinase 1 (PDK1) is highly expressed in breast cancer (BC) tissues and promotes tumor growth, but the underlying mechanisms of this process are unclear. Here, we investigated the effects of nuclear PDK1 on growth, migration and invasion in human BC cells.

Main Methods: The sub-cellular localization of PDK1 in BC cells was performed with subcellular fractionation followed by Western blot and immunofluorescence. The localization of PDK1 in breast normal tissue and breast duct carcinoma was detected by Immunohistochemistry. Then the protein-protein interaction between PDK1 and Importin β was verified by co-immunoprecipitation assay. Finally, the effects of nuclear PDK1 on cell proliferation, apoptosis, migration and invasion of BC cells were assessed.

Key Findings: In addition to its well-known sub-cellular localization, PDK1 was present in the nucleus of BC cells, and EGF treatment increased nucleus distribution of PDK1. Moreover, the level of nuclear PDK1 accumulation facilitated the growth of BC cells. We also found that the entry of PDK1 into nucleus mainly relied on the nuclear localization signal (NLS), and NLS mutation inhibited the entry of PDK1 into nucleus; as a result, the migration and invasion abilities of BC cells were impaired, and the number of apoptotic cells was significantly increased.

Significance: Our findings provided a new supplement to the sub-cellular localization of PDK1 in BC cells and uncovered the function of nuclear PDK1 in facilitating BC cells growth, migration and invasion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2020.117722DOI Listing
July 2020

Risk Factors for the Relapse of Graves' Disease Treated With Antithyroid Drugs: A Systematic Review and Meta-analysis.

Clin Ther 2020 04 2;42(4):662-675.e4. Epub 2020 Mar 2.

Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address:

Purpose: Antithyroid drugs (ATDs) are the first-line treatment for Graves' disease (GD). A common problem with ATD treatment is the high relapse rate after drug withdrawal. The goal of this study was to analyze the influencing factors for the relapse of GD patients treated with ATD by using a systematic review and meta-analysis, provide some predictive indexes for the susceptibility of GD recurrence, and then further explore some useful methods to decrease the GD relapse rate after ATD treatment.

Methods: Articles published in PubMed, EMBASE, The Cochrane Library, China National Knowledge Infrastructure, Wan Fang, and Chinese Biomedical Literature databases before January 2019 were collected. Patients newly diagnosed with GD, who were aged >16 years, were treated with ATD. Follow-up was then conducted for at least 12 months after ATD withdrawal. Only prospective or retrospective studies were eligible. The primary end point was the recurrence of GD during follow-up. All the data from the trials were analyzed via meta-analysis and meta-regression. p values < 0.05 were considered statistically significant, and statistical heterogeneity was assessed by using I statistics.

Findings: A total of 20 studies and 3242 patients were involved in this meta-analysis, with 1681 patients relapsed (incidence rate, 51.9%) during the follow-up time. Analysis of risk factors suggested that younger age (weighted raw mean difference [RMD], -3.51; 95% CI, -5.74 to -1.29), larger thyroid volume (RMD, 4.38; 95% CI, 1.68 to 7.08), bigger goiter size (1.94% risk; 95% CI, 0.43 to 3.46), higher free triiodothyronine level (RMD, 5.09; 95% CI, 4.42 to 5.77), and higher free thyroxine level (RMD, 4.21; 95% CI, 0.54 to 7.89) were associated with the higher relapse rate of GD. The block-replace ATD regimen (a fixed high dose of an ATD with levothyroxine supplementation to maintain euthyroidism) (risk ratio, 0.64; 95% CI, 0.52 to 0.78) exhibits a lower relapse rate than the titration regimen (an ATD used alone and dose adjusted according to thyroid function tests).

Implications: This analysis revealed that certain risk factors were associated with GD relapses such as younger age, larger goiter size or thyroid volume, and the higher free triiodothyronine or free thyroxine level in the diagnosing phase of GD. For patients with these clinical characteristics, early definitive treatment with radioactive iodine or surgery should be offered to those who are unlikely to achieve remission with ATDs only. In addition, more prospective cohort studies with different ATD regimens would help to determine the optimum ATD treatment for patients with GD. PROSPERO identifier: CRD 42019146825.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.clinthera.2020.01.022DOI Listing
April 2020

Study on Morphology and Rheological Property of Organoclay Dispersions in Soybean Oil Fatty Acid Ethyl Ester over a Wide Temperature Range.

ACS Omega 2020 Feb 24;5(4):1851-1861. Epub 2020 Jan 24.

PetroChina Jilin Oilfield Company, Songyuan, Jilin 138000, China.

This work attempted to establish the relationship between the dispersion morphology and the viscous flow behavior of clay dispersions in soybean oil fatty acid ethyl ester (FAEE) at 2 and 65 °C. The clays used in this study include raw montmorillonite (Mt) and three kinds of organoclays prepared by ion exchange modification of Mt by cetyltrimethylammonium chloride (OC16), dihexadecyldimethylammonium chloride (ODC16), and trihexadecylmethylammonium chloride (OTC16), respectively. The X-ray diffraction and water contact angle results demonstrated that greater alkyl chain number of surfactants led to greater interlayer space and stronger hydrophobicity of organoclays. Due to the good affinity of the surfactant and FAEE, OC16 exhibited the most stable dispersion in FAEE between 2-65 °C, which resulted in the best flat rheological property. The molecular structures of multiple chain surfactants were quite different from that of FAEE, resulting in weak affinity between organoclays (ODC16 and OTC16) and FAEE. The sheets of ODC16 and OTC16 tended to aggregate at 2 °C, forming a gel structure, thus significantly increasing the low shear rate viscosity (LSRV) and yield stress. At 65 °C, with the expansion of FAEE and the stronger thermal motion of sheets, the dispersions of ODC16 and OTC16 were improved, destroying the original gel structure and resulting in significant decreases in LSRV and yield stress. This study confirmed that stable clay/FAEE dispersions tended to exhibit flat rheology, which could serve as a basis for the application of clay/biodiesel dispersion in deep-water drilling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.9b03183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003210PMC
February 2020

Understanding the Origin of Selective Reduction of CO to CO on Single-Atom Nickel Catalyst.

J Phys Chem B 2020 Jan 9;124(3):511-518. Epub 2020 Jan 9.

Department of Chemistry , Duke University , Durham , North Carolina 27708 , United States.

Electrochemical reduction of CO to CO offers a promising strategy for regulating the global carbon cycle and providing feedstock for the chemical industry. Understanding the origin that determines the faradaic efficiency (FE) of reduction of CO to CO is critical for developing a highly efficient electrocatalyst. Here, by constructing a single-atom Ni catalyst on nitrogen-doped winged carbon nanofiber (NiSA-NWC), we find that the single-atom Ni catalyst possesses the maximum CO FE of over 95% at -1.6 V vs Ag/AgCl, which is about 30% higher than the standard Ni nanoparticles on the same support. The Tafel analysis reveals that the single-atom Ni catalyst has a preferred reduction of CO to CO and a slower rate for the hydrogen evolution reaction. We propose that the domination of singular Ni electronic states and limited hydrogen atom adsorption sites on the single-atom Ni catalyst lead to the observed high FE for CO reduction to CO.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpcb.9b09730DOI Listing
January 2020

[Molecular mechanism of macrophages derived from PGRN gene knockout mice inhibit invasion and migration of breast cancer cells].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2019 Sep;35(9):769-775

Ministry-of-Education Key Laboratory of Laboratory Medical Diagnostics, Chongqing Medical University, Chongqing 400016, China. *Corresponding author, E-mail:

Objective To explore the functions and mechanisms of macrophages derived from PGRN gene knockout (PGRN ) C57BL/6 mice in the invasion and migration of breast cancer cells. Methods Breast cancer cells were cultured in conditioned medium of macrophages derived from WT and PGRN mice. Transwell assay and scratch assay were used to detect the invasion and migration ability of cancer cells. Western blot analysis was used to detect the expression of E-cadherin and N-cadherin in cancer cells. Cytokine array, real-time quantitative PCR and ELISA were performed to investigate the differences of cytokines secreted by macrophages derived from WT and PGRN mice. Breast cancer cells were treated by the differentially expressed cytokine interleukin-6 (IL-6), and then the above methods were used to investigate its effect on cancer cells. Western blot analysis was used to verify the roles of NF-κB and JAK/STAT3 signaling pathways. Results The macrophages derived from PGRN mice blocked NF-κB signaling pathway, reduced IL-6 secretion, and inhibited the invasion and migration of breast cancer cells. IL-6 activated JAK/STAT3 signaling pathway to promote the invasion and migration of breast cancer cells. Conclusion The macrophages derived from PGRN mice can block the NF-κB and JAK/STAT3 signaling pathways, down-regulate IL-6 expression, and inhibit the invasion and migration of breast cancer cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
September 2019

The length and width of diatoms in drowning cases as the evidence of diatoms penetrating the alveoli-capillary barrier.

Int J Legal Med 2020 May 22;134(3):1037-1042. Epub 2019 Oct 22.

Ministry of Public Security, Guangzhou Forensic Science Institute & Key Laboratory of Forensic Pathology, Baiyun Avenue 1708, Baiyun District, Guangzhou, China.

Forensic diatom test has been considered as a significant tool for diagnosis of drowning. Most of the studies in this field discussed the methodology of extracting, enriching and detecting diatoms from different tissues and drowning media. There are few studies on the basic principle of diatom test which was based on the theory developed by forensic scientists many years ago. This study was designed to analyze the length and width of diatoms in different organs and drowning medium samples of drowning cases. This study is designed to find evidence of diatoms penetrating the alveoli-capillary barrier. Samples from 100 drowning cases were analyzed using the methodology we developed: the Microwave Digestion-Vacuum Filtration-Automated Scanning Electron Microscopy method (MD-VF-Auto SEM method). The results showed that the length and width of diatoms in the liver and kidney tissues are smaller than that of the lung tissues and water samples. Our studies also found that the pennate diatoms are easier to penetrate through the alveoli-capillary barrier, travel in the blood stream and finally deposit in the distant tissues including liver and kidney. These findings provided evidences to support the process of diatoms penetrating the alveoli-capillary barrier.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00414-019-02164-4DOI Listing
May 2020

Enhanced photoelectrocatalytic activity of direct Z-scheme porous amorphous carbon nitride/manganese dioxide nanorod arrays.

J Colloid Interface Sci 2019 Dec 11;557:644-654. Epub 2019 Sep 11.

School of Chemistry and Chemical Engineering/Institute of Clean Energy and Materials/Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, PR China. Electronic address:

Carbon nitride (CN) is a promising photocatalyst that can be applied in environmental remediation and energy conversion. However, the absorption range and charge separation efficiency of CN are still severely restricted for its large-scale practical applications. Herein, we demonstrate a simple thermal polymerization and electrodeposition method, followed by partial etching strategy to synthesize direct Z-scheme porous zinc oxide/amorphous carbon nitride/manganese dioxide hybrid core-shell nanorod array (denoted as P-ZnO/ACN/MnO) by encapsulating amorphous carbon nitride layers (ACN) and manganese dioxide nanosheets (MnO) on the zinc oxide nanorod arrays (denoted as ZnO). Interestingly, ZnO serves as the collector of charge carriers and MnO plays a significant role in protecting ACN from corrosion. The as-prepared Z-scheme P-ZnO/ACN/MnO heterojunction exhibits high photocurrent density of 5.2 mA cm at 0.6 V vs. Ag/AgCl, high photoconversion efficiency 0.98%, and universal photoelectrocatalytic degradation activity for degradation of organic dyes under visible light irradiation. The band gap energy and conduction band position of ZnO, ACN and MnO are calculated by UV-visible diffuse reflection and Mott-Schottky measurement, which strongly support the direct Z-scheme charge carrier migration mechanism. This finding provides an efficient strategy to construct highly active and stable CN-based Z-scheme photocatalytic system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2019.09.035DOI Listing
December 2019

Analysis of Regulatory T Cell Subsets and Their Expression of Helios and PD-1 in Patients with Hashimoto Thyroiditis.

Int J Endocrinol 2019 13;2019:5368473. Epub 2019 May 13.

Department of Endocrinology, Nanjing Medical University First Affiliated Hospital, Nanjing, China.

Hashimoto thyroiditis (HT) is an autoimmune disease that presumably arises consequent to loss of immune tolerance to autoantigen in thyroid. Regulatory T cells (Tregs) are considered to play a vital role in maintaining the immune balance, as they own intensive suppressive function. This study was undertaken to analyze numbers of Tregs and their expressions of Helios and PD-1 in HT patients. It also aimed to explore the relationship of these with thyroid function and specific autoantibodies. Peripheral blood mononuclear cells (PBMCs) were extracted from blood of 20 healthy controls (HC) and 42 HT patients with varying thyroid functions (10 overt hypothyroidism, 12 subclinical hypothyroidism, and 20 euthyroidism). We performed flow cytometry analysis in PBMCs to detect CD4CD25Foxp3Tregs and their subsets, including CD45ROFoxp3 activated Treg cells (aTregs), CD45ROFoxp3 resting Tregs cells (rTregs), and CD45ROFoxp3 secreting Treg cells (sTregs), as well as the expression of Helios and PD-1 on these cells. The results showed that the percentage of Tregs, aTregs was significantly lower in HT patients and it showed inverse correlation to thyroid function states, in comparison with these in healthy controls. In addition, patients with HT showed decreased expression of Helios in aTregs, while having increased expression of PD-1 in Tregs and sTregs. The levels of Tregs, aTregs, and Helios expressing aTregs were all negatively correlated with antithyroid antibodies. In conclusion, the deficiency of Tregs frequency and aberrant expressions of Helios and PD-1 may possibly contribute to thyroid immune damage in HT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2019/5368473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6535823PMC
May 2019

Prognostic Threshold of Neuroendocrine Differentiation in Gastric Carcinoma: a Clinicopathological Study of 945 Cases.

J Gastric Cancer 2019 Mar 20;19(1):121-131. Epub 2019 Mar 20.

Department of Pathology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.

Purpose: The significance of neuroendocrine differentiation (NED) in gastric carcinoma (GC) is controversial, leading to ambiguous concepts in traditional classifications. This study aimed to determine the prognostic threshold of meaningful NED in GC and clarify its unclear features in existing classifications.

Materials And Methods: Immunohistochemical staining for synaptophysin, chromogranin A, and neural cell adhesion molecule was performed for 945 GC specimens. Survival analysis was performed using the log-rank test and univariate/multivariate models with percentages of NED (P) and demographic and clinicopathological parameters.

Results: In total, 275 (29.1%) cases were immunoreactive to at least 1 neuroendocrine (NE) marker. GC-NED was more common in the upper third of the stomach. P, and Borrmann's classification and tumor, lymph node, metastasis stages were independent prognostic factors. The cutoff P was 10%, beyond which patients had significantly worse outcomes, although the risk did not increase with higher P. Tumors with ≥10% NED tended to manifest as Borrmann type III lesion with mixed/diffuse morphology and poorer histological differentiation; the NE components in this population mainly grew in insulae/nests, which differed from the predominant growth pattern (glandular/acinar) in GC with <10% NED.

Conclusions: GC with ≥10% NED should be classified as a distinct subtype because of its worse prognosis, and more attention should be paid to the necessity of additional therapeutics for NE components.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5230/jgc.2019.19.e9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441775PMC
March 2019

Forensic entomology application in China: Four case reports.

J Forensic Leg Med 2019 Apr 5;63:40-47. Epub 2019 Mar 5.

Department of Forensic Medicine, Soochow University, Ganjiang East Road, Suzhou, China. Electronic address:

We present four cases that use entomological data to estimate postmortem interval (PMI). In the first case, the body was found in an outdoor environment at 26 °C and was at the fresh stage. However, the PMI estimated using maggots collected from the corpse was more than 68 h. This was certainly an incorrect estimation because the degree of the body decomposition was light, and it may have been caused by flies invading the corpse before death occurred. In the second case, a corpse of an adult male was found in a semi-closed room, and the body was highly decomposed and mummified. In this case, we used Dermestes maculatus DeGeer (Coleoptera: Dermestidae) to successfully estimate the PMI. The third case involved a female body discovered in her room after she committed suicide by consuming excessive clozapine and alcohol. The PMI estimated through entomological data did not match the time of death that the other evidence indicated. Thus, we speculated that clozapine and alcohol may have influenced the development of fly larvae. The fourth case occurred in the winter. The murderer killed the victim and burned and dumped the corpse. However, the burned body still attracted necrophagous insects and we successfully calculated the PMI by using insect evidence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jflm.2019.03.001DOI Listing
April 2019

Analysis of false-positive results of diatom test in the diagnosis of drowning-would not be an impediment.

Int J Legal Med 2019 Nov 15;133(6):1819-1824. Epub 2019 Feb 15.

Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University & Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Zhongshan 2nd Road 74, Yuexiu District, Guangzhou, 510080, China.

Diatom test has been a significant tool for the diagnosis of drowning. The reliability of the diatom test is still in strong dispute in the field of forensic science because of the false-positive results. This study was designed to quantitatively compare the numbers of the diatoms in false-positive cases and true drowning cases. Diatom samples from 64 victims were used in this study: 32 cases are confirmed drowning victims and other 32 cases died from non-drowned death. Samples were subject for the diatom test those were analyzed by the microwave digestion-vacuum filtration-automated scanning electron microscopy method (MD-VF-Auto SEM method) that we developed before. The results did show that there are false-positive diatoms detected in the liver and kidney tissues of non-drowned bodies: 6/20 in liver tissues and 7/20 in kidney tissues. However, the quantitative studies showed that there are statistical differences with the numbers of diatoms in the false-positive cases and in the true drowning cases. Diatom test of single organ is difficult for us to distinguish the sources of the diatoms detected. Therefore, comprehensive analysis of multiple organs would be more useful for the diagnosis of drowning.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00414-019-02021-4DOI Listing
November 2019

Mechanisms for Abscisic Acid Inhibition of Primary Root Growth.

Plant Signal Behav 2018 6;13(9):e1500069. Epub 2018 Aug 6.

a State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology , College of Life Sciences, Henan University , Kaifeng , China.

Abscisic acid (ABA) plays pivotal roles in plant growth and development and in responses to diverse stresses. It also modulates the growth of primary and lateral roots. Much evidence indicated that key cellular components auxin, ethylene, PLETHs, reactive oxygen species and Ca are involved in the regulation of ABA suppression of root elongation. In this review, we summary the molecular mechanism for ABA inhibiting primary root growth, focusing on the roles of these components in Arabidopsis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/15592324.2018.1500069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204825PMC
June 2019

The diagnostic value of TROP-2, SLP-2 and CD56 expression in papillary thyroid carcinoma.

Eur Arch Otorhinolaryngol 2018 Aug 27;275(8):2127-2134. Epub 2018 Jun 27.

Department of Endocrinology, Jiangsu Province Hospital and Nanjing Medical University First Affiliated Hospital, Nanjing, China.

Objective: The study aimed to explore some novel diagnostic biomarkers for papillary thyroid carcinoma (PTC) by identifying the different expression of TROP-2, SLP-2 and CD56 in benign and malignant thyroid lesions.

Methods: We evaluated the mRNA expressions of TROP-2 and SLP-2 in fine needle aspirates (FNAs) which contained 10 PTCs and 10 benign follicular adenomas (FAs) using quantitative real-time PCR (qRT-PCR). Immunohistochemical (IHC) staining of TROP-2, SLP-2 and CD56 was also performed on postoperative samples of 30 PTCs and 29 FAs. Membranous or cytoplasmic staining in > 10% of cells was considered as positive. Diagnostic sensitivity, specificity, positive predictive value, negative predictive value (NPV) and diagnostic accuracy of these three biomarkers were carried out. We further analyzed the associations between the clinical features and the expressions of markers in PTCs.

Results: The mRNA expressions of both TROP-2 and SLP-2 were increased substantially in PTCs in comparison with those in FAs (P < 0.05). Similarly, IHC for these two proteins demonstrated higher positive staining in PTCs than in FAs (96.5% vs. 12.5% for TROP-2, 83.3% vs. 20.7% for SLP-2, P < 0.05). Conversely, CD56 expression was lost with 86.7% of PTCs. In identifying malignancy, TROP-2 was the most sensitive marker and CD56 was the most specific one. When the markers were combined, the sensitivity and NPV increased to 100% and had better diagnostic accuracy. However, no association was found between biomarker expressions and clinicopathological factors in PTCs.

Conclusions: We found that TROP-2, SLP-2 and CD56 were effective diagnostic markers for PTC, especially when they were combined to use.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00405-018-5045-xDOI Listing
August 2018

The SlNAC8 gene of the halophyte Suaeda liaotungensis enhances drought and salt stress tolerance in transgenic Arabidopsis thaliana.

Gene 2018 Jul 6;662:10-20. Epub 2018 Apr 6.

College of Life Sciences, Liaoning Normal University; Key Laboratory of Plant Biotechnology of Liaoning Province, Dalian, Liaoning 116081, PR China. Electronic address:

NAC (NAM, ATAF1/2 and CUC) transcription factors play an important role in resisting abiotic stress in plants. In this study, a novel NAC gene, designated SlNAC8 from Suaeda liaotungensis K. was characterized. SlNAC8 protein is localized in the nucleus, and the yeast one-hybrid screening showed that it contains an activation domain in its C-terminus and functions as a transcriptional activator. Gene expression analysis revealed that it is induced by drought and salt stress. Arabidopsis plants overexpressing SlNAC8 demonstrated enhanced tolerance to drought and salt stress, showing significant advantages in seed germination, root growth, shoot growth, and survival rate compared with controls. Moreover, transgenic plants had a significantly higher proline concentration, antioxidant enzyme activity (superoxide dismutase, peroxidase, and catalase), and level of chlorophyll fluorescence than wild-type, and a significantly lower malondialdehyde concentration and electrolyte leakage under drought and salt stress. The overexpression of SlNAC8 in transgenic plants also enhanced the expression of stress-responsive genes such as RD20, GSTF6, COR47, RD29A, RD29B, and NYC1. In summary, SlNAC8, as a transcription factor, may change the physiological-biochemical characteristic of plants by regulating the expression of stress-responsive genes and enhance the drought and salt stress tolerance of plants. SlNAC8 can be utilized for developing drought and salinity tolerance in crop plants through genetic engineering.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2018.04.012DOI Listing
July 2018

Associations Between Three CTLA-4 Polymorphisms and Hashimoto's Thyroiditis Risk: An Updated Meta-Analysis with Trial Sequential Analysis.

Genet Test Mol Biomarkers 2018 Apr 20;22(4):224-236. Epub 2018 Feb 20.

Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University , Nanjing, China .

Aims: In this article, we conducted an updated meta-analysis with trial sequential analysis (TSA) to refine the associations between three common single nucleotide polymorphisms (SNPs) in the CTLA-4 gene (+49A/G, CT60, and -318C/T) and Hashimoto's thyroiditis (HT).

Methods: Statistical association analyses were performed using four genetic models, including the allelic, codominant, dominant, and recessive models with the Revman 5.3, Stata 14.0, and TSA 0.9 software. For quality evaluation, the Newcastle-Ottawa Scale was used.

Results: Our meta-analysis included 29 independent studies with low risk of bias that involved 3614 cases and 8839 controls. The pooled results indicated a significant association between the +49A/G polymorphism and an increased risk of HT in all four genetic models. Furthermore, the TSA demonstrated that the evidence of this association was robust and credible. Subgroup analysis revealed a significantly higher risk of HT in Asians compared with Caucasians associated with the +49A/G polymorphism. Surprisingly, in contrast to the results with adults, we did not find any significant association when analyzing the pediatric subgroup. For the CT60 polymorphism, a significant association with risk of HT was detected overall, and subgroup analysis revealed that this association was significant in the Asian subgroup, but not in the Caucasian subgroup. No statistically significant associations were detected in any of the investigated genetic models for the -318C/T polymorphism. However, the results of the TSA suggested that the sample sizes used for the CTLA-4 CT60 and -318C/T SNPs were insufficient.

Conclusion: Our meta-analysis showed significant associations between the risk of HT and both the +49A/G and CT60 polymorphisms, but not the -318C/T polymorphism. In addition, the TSA results indicated that CTLA-4 +49A/G should be considered as a biomarker for HT, whereas both the CT60 and -318C/T SNPs warrant confirmation by further studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/gtmb.2017.0243DOI Listing
April 2018

Functional polymorphism rs3783553 in the 3'-untranslated region of IL-1A increased the risk of ischemic stroke: A case-control study.

Medicine (Baltimore) 2017 Nov;96(46):e8522

Department of Radiology, the Second People's Hospital of Yunnan Province, Kunming, Yunnan, China.

Accumulating evidence indicates interleukin-1 (IL-1) is a critical mediator of inflammatory responses in ischemic stroke (IS). The aim of this study was to investigate whether rs3783553 in the 3'-untranslated region of IL-1A was associated with the risk of IS. In this hospital-based case-control study, we genotyped the rs3783553 using polymerase chain reaction in 316 patients with IS and 332 age, sex, and ethnicity-matched controls. Plasma level of IL-1α was measured by enzyme-linked immunosorbent assay. The relative luciferase activities were measured by the Dual Luciferase assay system. The presence of ins/ins genotype was associated with higher odds ratios (ORs) of IS compared with del/del genotype (ins/ins vs del/del: adjusted OR 1.77, 95% confidence interval [CI] 1.06-2.98; recessive model: adjusted OR 1.69, 95% CI 1.06-2.70). The higher risk of IS was also observed in allele comparison (adjusted OR 1.29, 95% CI 1.00-1.65). Multivariate logistic regression analysis showed that age, hypertension, total cholesterol, triglyceride, low-density lipoprotein, and rs3783553ins/ins genotypes were independent risk factors for IS. Plasma level of IL-1α was higher among IS patients compared with controls (P = .03). Notably, IS patients with the TTCA/TTCA genotype had a higher level of IL-1α compared with those with the del/del genotype (P = .01). Luciferase reporter assay showed that the vector containing the TTCA del allele exhibited a reduced transcriptional activity in the presence of miR-122 and miR-378. These findings indicate that IL-1A rs3783553 ins/ins genotype may increase the susceptibility to IS, possibly by interrupting the binding site of miR-122 and miR-378.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000008522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704800PMC
November 2017

The Diagnostic Value of Quantitative Assessment of Diatom Test for Drowning: An Analysis of 128 Water-related Death Cases using Microwave Digestion-Vacuum Filtration-Automated Scanning Electron Microscopy.

J Forensic Sci 2017 Nov 15;62(6):1638-1642. Epub 2017 Feb 15.

Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Zhongshan 2nd Road 74, Yuexiu District, Guangzhou, 510080, China.

The value of diatom test for the diagnosis of drowning remains controversial. The conventional forensic diatom test with low sensitivity is not a useful tool to provide accurate information about diatom in the tissues and organs. To improve the sensitivity of the diatom test, we developed a novel method called the Microwave Digestion-Vacuum Filtration-Automated Scanning Electron Microscopy (MD-VF-Auto SEM) method which resulted in a high recovery of diatoms. In this article, we analyzed 128 water-related death cases. Our results showed that the MD-VF-Auto SEM method could achieve a much higher positive rate (0.97) in drowning cases. Large amounts of diatoms are retained, even concentrated, in the lung tissues during the intense breathing movement in drowning process. This might be useful for the diagnosis of drowning. Our research indicates that the MD-VF-Auto SEM method would be a valuable methodology in the study of diatom test for the forensic community.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1556-4029.13455DOI Listing
November 2017