Publications by authors named "He Liu"

803 Publications

Urinary levels of dimethoate, bisphenol A and benzo[a]pyrene in first-year students of Hohai University from different geographical regions.

BMC Public Health 2021 Sep 16;21(1):1692. Epub 2021 Sep 16.

Core Laboratory, Sir Run Run Hospital, Nanjing Medical University, Nanjing, 211166, China.

Background: The objective of this study was to detect the urinary levels of dimethoate, benzo(a) pyrene (BaP), and bisphenol A (BPA) in first-year Hohai University students with different geographic origins.

Methods: First-morning urine samples were collected from 540 healthy freshmen aged 17 to 19 years. Chemical levels were measured using β-glucuronidase hydrolysis followed by a high-performance liquid chromatography-tandem mass spectrometry-based method. Geometric means (GMs) of these three chemicals are presented by body mass index (BMI) and location in a volume-based and creatinine-standardized way.

Results: GM concentrations of omethoate, BPA and 3-OHBaP were 9.47 μg/L (10.80 μg/g creatinine), 3.54 μg/L (4.04 μg/g creatinine) and 0.34 ng/L (0.39 ng/g creatinine), respectively. The GM concentration of omethoate in males was significantly higher than that in females. The individuals with a BMI higher than 23.9 had higher GM concentrations of omethoate, BPA, and 3-OHBaP. The inhabitants of Southwest China had significantly lower GM concentrations of omethoate, BPA, and 3-OHBaP than those who lived in other locations in China.

Conclusion: The average level of environmental chemical accumulation in freshmen is lower in Southwest China and differs in youth who live in different regions. In addition, obesity is correlated with higher toxin levels in youth.
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http://dx.doi.org/10.1186/s12889-021-11726-4DOI Listing
September 2021

Facile fabrication of soy protein isolate-functionalized nanofibers with enhanced biocompatibility and hemostatic effect on full-thickness skin injury.

Nanoscale 2021 Sep 16. Epub 2021 Sep 16.

Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Disease, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, P. R. China.

Extensive full-thickness skin defect lacks self-healing ability. Tissue engineering wound dressing is considered as the most promising approach to promote wound healing. In this study, a series of biocompatible and hemostatic nanofiber dressings were fabricated. Soy protein isolate (SPI) and poly(L-lactic acid) (PLLA) solutions were mixed in certain proportions for high-voltage electrospinning. The obtained products were coded as SPNF- ( = 100, 80, 60 and 40, corresponding to the weight percentage of PLLA solution). We found that SPNF- ( = 100, 80, 60 and 40) could facilitate the adhesion and spread of L929 cells. In particular, SPNF-80 was capable of promoting fibroblast proliferation and diminishing inflammation. Compared with the neat PLLA film (SPNF-100), the biosafety and hemostatic effect of SPNF-80 got significantly improved. The hemostatic effect of SPNF-80 was comparable with that of a commercial gelatin sponge. wound healing assay demonstrated that SPNF-80 could accelerate the wound healing process by enhancing vascularization, re-epithelization and collagen formation. In conclusion, our results reveal that SPNF- has good biocompatibility and hemostatic effect, and exhibits great application potential in wound healing.
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http://dx.doi.org/10.1039/d1nr03430hDOI Listing
September 2021

Renin-angiotensin system inhibitors and clinical outcomes in patients with atrial fibrillation and heart failure: a propensity score-matched study from the Chinese Atrial Fibrillation Registry.

J Int Med Res 2021 Sep;49(9):3000605211041439

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University; National Clinical Research Center for Cardiovascular Diseases; Beijing Advanced Innovation Center for Big Data-Based Precision Medicine for Cardiovascular Diseases, Beijing, China.

Objective: The effect of renin-angiotensin system inhibitors (RASIs) in patients with heart failure (HF) and atrial fibrillation (AF) remains unclear. This study aimed to investigate associations between RASI use and all-cause mortality and cardiovascular outcomes in patients with AF and HF.

Methods: Using data from the China Atrial Fibrillation Registry study, we included 938 patients with AF and HF with a left ventricular ejection fraction <50%. Cox regression models for RASIs vs. non-RASIs with all-cause mortality as the primary outcome were fitted in a 1:1 propensity score-matched cohort. A sensitivity analysis was performed by using a multivariable time-dependent Cox regression model. As an internal control, we assessed the relation between β-blocker use and all-cause mortality.

Results: During a mean follow-up of 35 months, the risk of all-cause mortality was similar in RASI users compared with non-users (hazard ratio: 0.92; 95% confidence interval: 0.67-1.26). Similar results were obtained in the sensitivity analysis. In contrast, β-blocker use was associated with significantly lower all-cause mortality in the same population.

Conclusions: RASI use was not associated with better outcomes in patients with AF and HF in this prospective cohort, which raises questions about their value in this specific subset.Trail Registration: ChiCTR-OCH-13003729.
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http://dx.doi.org/10.1177/03000605211041439DOI Listing
September 2021

Parathyroid disorder and concomitant thyroid cancer in patients with multiple endocrine neoplasia type 1: A retrospective cohort study.

Medicine (Baltimore) 2021 Sep;100(36):e27098

Department of Ultrasound, Chinese Academy of Medical Sciences & Peking Union Medical College Hospital, Beijing, China.

Abstract: This study aimed to determine the rates and characteristics of parathyroid disorder and thyroid cancer in patients with multiple endocrine neoplasia type 1 vs sporadic primary hyperparathyroidism (SPHP) undergoing parathyroidectomy.Patients with multiple endocrine neoplasia type 1-associated primary hyperparathyroidism (MPHP) or SPHP who underwent initial or reoperative parathyroid exploration from 1999 to 2019 were identified via a clinical database. The data for MPHP patients (n = 15) were compared to those of a selected 2:1 age- and sex-matched SPHP cohort (n = 30) who all underwent thyroidectomy for concurrent thyroid nodules.Compared with that of the SPHP group, the parathyroid hormone level of the MPHP group was much higher (470.67 ± 490.74 pg/mL vs 217.77 ± 165.60 pg/mL, P = .001). Multiglandular parathyroid disease (6/15 [40%] vs 3/30 [10%], P = .026) and more hyperplasia (7/15 [46.7%] vs 5/30 [16.7%], P = .039) were found in the MPHP group, and more parathyroid lesions presented as a round shape (long/short meridian < 2) by ultrasound (16/20 [80%] vs 8/31 [25.8%], P < .001). Regarding thyroid nodules, there was no difference in the rate of histologic thyroid cancer, but more thyroid cancer was found in the last 5 years among the MPHP cases (5/9 [55.6%] vs 3/18 [16.7%], P = .052).Multiglandular parathyroid disease and hyperplasia were more frequent in the MPHP cohort than in the SPHP cohort, and the parathyroid lesions usually presented with a round shape on ultrasonography. More concurrent thyroid cancer was found in MPHP than SPHP patients over the previous 5 years.
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http://dx.doi.org/10.1097/MD.0000000000027098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428741PMC
September 2021

Characterization of the structure and properties of the isolating interfacial layer of oil-water emulsions stabilized by soy hull polysaccharide: Effect of pH changes.

Food Chem 2021 Sep 2;370:131029. Epub 2021 Sep 2.

College of Food Science and Technology, Bohai University, Jinzhou 121013, China; Liaoning Province Grain and Cereal Food Bio-efficient Transformation Engineering Research Center, Bohai University, Jinzhou 121013, China. Electronic address:

The effect of pH on the microstructure and properties of the soy hull polysaccharide interfacial layer was determined. The particle size at pH 2.0 was the largest (36.7 μm), whereas the absolute ζ-potential was the smallest. The protein content was the lowest at pH 2.0 and 9.0 and peaked around pH 4.0-5.0 (77.7%). The ordered secondary protein structure content under low pH conditions was greater than that under high pH conditions and the stability of the interfacial layer was higher at high pH, whereas the emulsion viscosity decreased by two orders of magnitude between pH 2.0 and 9.0. It appears that low pH reduced the thermal stability and increased the apparent viscosity of the emulsion by increasing the structural order of the protein in the interfacial layer. These findings lay the foundation for future work to reveal the key components and characteristic structures of soy hull polysaccharide that affect interfacial stability.
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http://dx.doi.org/10.1016/j.foodchem.2021.131029DOI Listing
September 2021

Relationship between serum lipid levels and ischemic stroke in patients with atrial fibrillation: a nested case-control study based on the China Atrial Fibrillation Registry.

BMC Cardiovasc Disord 2021 09 8;21(1):424. Epub 2021 Sep 8.

Department of Cardiology, Beijing Anzhen Hospital, National Clinical Research Center for Cardiovascular Diseases, Capital Medical University, 2 Anzhen Road, Chaoyang District, Beijing, China.

Background: Atrial fibrillation (AF) is an important risk factor for acute ischemic stroke.

Methods: A nested case-control study was conducted among patients diagnosed with AF, whose information was acquired from the prospective China Atrial Fibrillation Registry (China-AF), from August 2011 to December 2018.

Results: This study compared patients with stroke group (n = 145) with a matched control group (n = 577). Demographic data were similar except for body mass index (BMI), diastolic blood pressure (DBP) which were higher, and new oral anticoagulant (NOAC) treatment rate which was lower in the stroke group (all P < 0.05). Baseline median [IQR] levels of including triglyceride (TG) were higher in the stroke group (21.96 [16.74, 21.52], mg/dL) than the control group (19.62 [14.76, 27.36], mg/dL) (P = 0.012), while the total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were similar between the two groups. Elevated TG and HDL-C were positively associated with ischemic stroke (OR 1.01, 95% CI 1.00-1.02, P = 0.032; OR 1.03, 95% CI 1.00-1.05, P = 0.025), after adjustment for BMI, systolic blood pressure, DBP, CHADS-VASc score, HAS-BLED score, NOAC, LDL-C and HDL-C. However, NOAC (OR 0.20, 95% CI 0.05-0.84, P = 0.029) could decrease the likelihood of ischemic stroke in patients with AF. In subgroup analysis, higher TG level remained significantly associated with ischemic stroke for AF patients without a history of smoking (OR 1.26, 95% CI 1.02-1.55, P = 0.028).

Conclusion: Higher level of TG and HDL-C were positively associated with ischemic stroke in patients with AF.
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http://dx.doi.org/10.1186/s12872-021-02237-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425053PMC
September 2021

Micro-computed Tomography Evaluation of Root Canal Filling Quality of Mandibular Molars Obturated for 54 Months.

J Endod 2021 Sep 4. Epub 2021 Sep 4.

Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Canada.

Introduction: The present study evaluated the percentage volume of voids in root canals of mandibular molars that had been obturated for 54 months.

Methods: Thirty extracted human mandibular molars were instrumented and debrided. The teeth were assigned to three groups (n=10) according to the filling technique and sealer used: single-cone technique using AH Plus sealer (AHS) or EndoSequence BC sealer (BCS), and warm vertical compaction technique using AH Plus sealer (AHW). The specimens were stored at 37 °C and 100% humidity. Micro-computed tomography was used to scan each specimen one day and 54 months after obturation. Data were analyzed using one-way ANOVA and paired t-test.

Results: The percentage volume of voids in the teeth one day after obturation in the AHS group was higher than in the BCS group and AHW group (P < 0.05). After 54 months, the proportion of voids decreased in all groups (P < 0.05). No significant difference was observed between the AHS group and BCS group after 54 months. Teeth in the AHW group contained fewer voids than the AHS group (P < 0.05).

Conclusions: Voids in root canal filling were reduced 54 months after obturation. The warm vertical compaction technique achieved better root canal filling quality in mandibular molars than the single-cone technique when using AH Plus sealer after long-term storage at 100% humidity.
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http://dx.doi.org/10.1016/j.joen.2021.08.015DOI Listing
September 2021

Fault Estimation and Control for Unknown Discrete-Time Systems Based on Data-Driven Parameterization Approach.

IEEE Trans Cybern 2021 Sep 3;PP. Epub 2021 Sep 3.

This study investigates the problem of fault estimation and control for unknown discrete-time systems. Such a problem was first formulated as an multiobjective optimization problem. Then, a data-driven parameterization controller design method was proposed to optimize both fault estimation and robust control performances. In terms of the single-objection control problem, necessary and sufficient conditions for designing the suboptimal controller were presented, and the performance index optimized by the developed data-driven method was shown to be consistent with that of the model-based method. In addition, by introducing additional slack variables into the controller design conditions, the conservatism of solving the multiobjective optimization problem was reduced. Furthermore, contrary to the existing data-driven controller design methods, the initial stable controller was not required, and the controller gain was directly parameterized by the collected state and input data in this work. Finally, the effectiveness and advantages of the proposed method are shown in the simulation results.
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http://dx.doi.org/10.1109/TCYB.2021.3107425DOI Listing
September 2021

Development and Validation of Nomogram Prediction Model for Postoperative Sleep Disturbance in Patients Undergoing Non-Cardiac Surgery: A Prospective Cohort Study.

Nat Sci Sleep 2021 24;13:1473-1483. Epub 2021 Aug 24.

Department of Anesthesiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou City, Jiangsu Province, People's Republic of China.

Purpose: To develop a risk prediction nomogram of postoperative sleep disturbance (PSD) in patients undergoing non-cardiac surgery.

Patients And Methods: Data on 881 consecutive patients who underwent non-cardiac surgery at the Affiliated Hospital of Xuzhou Medical University between June 2020 and April 2021 were prospectively collected. Of these, we randomly divided 881 non-cardiac patients into two groups, training cohort (n = 617) and validation cohort (n = 264) at the ratio of 7:3. Characteristic variables were selected based on the data of training cohort through least absolute shrinkage and selection operator (LASSO) regression. Multivariate logistic regression was used to identify the independent risk factors associated with PSD that then were incorporated into the nomogram. The predictive performance of the nomogram was measured by concordance index (C index), receiver operating characteristic (ROC) curve, and calibration with 1000 bootstrap samples to decrease the over-fit bias.

Results: PSD was found in 443 of 617 patients (71.8%) and 190 of 264 patients (72.0%) in the training and validation cohorts, respectively. The perioperative risk factors associated with PSD were female sex, anxiety, dissatisfaction of ward environment, absence of combined regional nerve block, postoperative nausea and vomiting (PONV), the longer duration stayed in post anesthesia care unit (PACU), the higher dose of midazolam and sufentanil, the higher postoperative numeric rating score for pain (NRS) score. Incorporating these 9 factors, the nomogram achieved good concordance indexes of 0.82 (95% confidence interval [CI], 0.78-0.85) and 0.80 (95% CI, 0.74-0.85) in predicting PSD in the training and validation cohorts, respectively, and obtained well-fitted calibration curves. The sensitivity and specificity (95% CIs) of the nomogram were calculated, resulting in sensitivity of 74.0% (70.0-78.2%) and 75.3% (68.4-81.7%) and specificity of 79.3% (72.5-85.2%) and 70.3% (58.4-80.7%) for the training and validation cohorts, respectively. Patients who had a nomogram score of less than 262 or 262 or greater were considered to have low or high risks of PSD presence, respectively.

Conclusion: The proposed nomogram achieved an optimal prediction of PSD in patients undergoing non-cardiac surgery. The risks for an individual patient to harbor PSD can be determined by this model, which can lead to a reasonable preventive and treatment measures.
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http://dx.doi.org/10.2147/NSS.S319339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403031PMC
August 2021

Streptomyces luteolifulvus sp. nov., a novel actinomycete isolated from soil in Nanjing, China.

Antonie Van Leeuwenhoek 2021 Aug 30. Epub 2021 Aug 30.

School of Life Science and Technology, Harbin Institute of Technology, No. 2 Yikuang Street, Nangang District, Harbin, 150080, People's Republic of China.

During the investigation of exploring potential sources of novel species and natural bioactives, a novel actinomycete, designated strain HIT-DPA4, was isolated from a soil sample, which was collected from Nanjing, Jiangsu Province, PR China and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities and the result of phylogenetic analysis, strain HIT-DPA4 was most closely related to Streptomyces cyaneus CGMCC 4.1671, and shared the highest sequence similarity of 98.76%. In addition, the cell walls of the species HIT-DPA4 contained LL-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysates were identified as glucose and ribose, and the principal phospholipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and phosphatidylmonomethylethanolamine. MK-9(H) and MK-9(H) were predominant menaquinones; and C, anteiso-C and C as major cellular fatty acids of the organism HIT-DPA4. Gene Ontology database analysis and antiSMASH server predicted results displayed that strain HIT-DPA4 was a promising classification units, which has various types of functions and contains multiple biosynthetic gene clusters with the similarity more than 80%. Multilocus sequence analysis (MLSA) of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) illustrated that Streptomyces luteolifulvus formed a separate branch in the genus Streptomyces. However, a combination of low level of DNA-DNA relatedness and physiological properties indicated that strain HIT-DPA4 can be distinguished from its phylogenetically related species Streptomyces cyaneus CGMCC 4.1671. Moreover, gene synteny research could be further differed organism HIT-DPA4 from similarity species. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces luteolifulvus sp. nov. is proposed. The type strain is HIT-DPA4 (= CGMCC 4.7558 = TISTR 2751).
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http://dx.doi.org/10.1007/s10482-021-01643-2DOI Listing
August 2021

Mechanistic Insight into Copper-Mediated Trifluoromethylation of Aryl Halides: The Role of CuI.

J Am Chem Soc 2021 Sep 25;143(35):14367-14378. Epub 2021 Aug 25.

Key Laboratory of Organofluorine Chemistry, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, P.R. China.

The synthesis, characterization, and reactivity of key intermediates [Cu(CF)(X)]Q (X = CF or I, Q = PPh) in copper-mediated trifluoromethylation of aryl halides were studied. Qualitative and quantitative studies showed [Cu(CF)]Q and [Cu(CF)(I)]Q were not highly reactive. Instead, a much more reactive species, ligandless [CuCF] or DMF-ligated species [(DMF)CuCF], was generated in the presence of excess CuI. On the basis of these results, a general mechanistic map for CuI-promoted trifluoromethylation of aryl halides was proposed. Furthermore, on the basis of this mechanistic understanding, a HOAc-promoted protocol for trifluoromethylation of aryl halides with [PhP][Cu(CF)] was developed.
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http://dx.doi.org/10.1021/jacs.1c07408DOI Listing
September 2021

Mechanism of interactions between soyasaponins and soybean 7S/11S proteins.

Food Chem 2021 Aug 13;368:130857. Epub 2021 Aug 13.

College of Food Science and Technology, Bohai University, Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning 121013, China. Electronic address:

In this study, the proteins glycinin (11S) and β-conglycinin (7S) were mixed with soyasaponin (Ssa) Ab/Bb to form a composite system. We used fluorescence and synchronous fluorescence spectra to demonstrate the changes in the surrounding environment and the structure of the proteins. Dynamic interface behavior analysis showed the possible interface behavior induced by the composite system. The interactions between Ssa and the proteins, along with the mode of action, were analyzed by molecular docking. The interactions between Ssa and soy protein increased with the change in concentration. The interactions between the two proteins were mediated by tryptophan (Trp) and primarily involved hydrogen bonds, which changed the microenvironment and loosened the protein structure. These results helped in understanding the mechanism underlying the interactions between Ssa Ab/Bb and 7S/11S. Furthermore, these results highlighted the theoretical fundamentals for the future applications of composite systems as surfactants in the food industry.
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http://dx.doi.org/10.1016/j.foodchem.2021.130857DOI Listing
August 2021

Stimulation and Sequestration Mechanism of CO Waterless Fracturing for Continental Tight Oil Reservoirs.

ACS Omega 2021 Aug 4;6(32):20758-20767. Epub 2021 Aug 4.

Beijing Key Laboratory for Greenhouse Gas Storage and CO2-EOR, China University of Petroleum (Beijing), Beijing 102249, China.

CO fracturing is a promising technology for oil field development in tight, continental deposits, with potential advantages of enhanced oil recovery (EOR), CO sequestration, and water conservation. Compared with CO-EOR techniques, such as CO huff and puff and CO flooding, CO can interact with reservoir rock and fluid under higher pressure conditions during fracturing, resulting in CO stimulation and sequestration effects that differ from those that occur during conventional CO-EOR. In this paper, the CO interactions between CO and reservoirs in continental tight oil reservoirs under fracturing conditions are systematically studied through laboratory experiments. The results show that under high pressure, CO effectively changes the pore structure through the extraction of hydrocarbons, dissolution of the rock matrix, and migration of minerals. CO dissolution of the rock matrix can significantly increase the number and complexity of fractures. Furthermore, CO has a higher solubility in formation fluid under high-pressure conditions. Given the higher pressures, CO forms a miscible phase with crude oil, diffuses more deeply into the formation, and reacts fully with the reservoir minerals and fluid during CO fracturing. Accordingly, CO can improve the permeability of the reservoir and flowability of crude oil significantly. Hence, CO fracturing can enhance oil recovery and CO sequestration more effectively. Core displacement experiments indicate that oil recovery of CO soaking process after CO fracturing is 36%, which is 12% and 9% higher than those of CO huff and puff and CO flooding with 5 pore volume, respectively. Field tests show that average oil production after CO fracturing is 1.42 times higher than that after CO flooding, which further validates the advantage of CO fracturing and demonstrates its huge application potential.
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http://dx.doi.org/10.1021/acsomega.1c01059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374915PMC
August 2021

DAPK2 activates NF-κB through autophagy-dependent degradation of I-κBα during thyroid cancer development and progression.

Ann Transl Med 2021 Jul;9(13):1083

Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Discipline Construction Research Center of China Hospital Development Institute, Shanghai Jiao Tong University, Shanghai, China.

Background: Death-associated protein kinase 2 (DAPK2) is a serine/threonine kinase, which has been implicated in autophagy and apoptosis. DAPK2 functions as a tumor suppressor in various cancers. However, the role of DAPK2 in thyroid cancer (TC) is unclear.

Methods: RNA sequencing of human TC samples was performed to identify differentially expressed genes that may play a role in TC development. The messenger RNA (mRNA) expression of DAPK2 was verified by quantitative real-time polymerase chain reaction (qRT-PCR). To investigate the role of DAPK2 in TC development, DAPK2 was knocked down and overexpressed in a TTA1 cell line. The effect of DAPK2 on cell proliferation, sensitization of TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis and tumor growth was examined. The effect of DAPK2 on autophagy and NF-κB activation was investigated to address the underlying mechanism.

Results: DAPK2 was upregulated in TC. Knockdown of DAPK2 in TTA1 cells led to reduced cell proliferation, sensitization of TRAIL-induced apoptosis, and restricted tumor growth both and , while overexpression of DAPK2 exhibited the opposite effect. Mechanistically, DAPK2 promoted autophagy as demonstrated by the accumulation of microtubule-associated protein 1A/1B-light chain 3 (LC3)-II, which correlated with the level of nuclear factor-κB (NF-κB) activation. Knockdown of inhibitory-κBα (I-κBα) in short hairpin (sh) DAPK2 TTA1 cells restored the activity of NF-κB, suggesting DAPK2 activated NF-κB through autophagy-mediated I-κBα degradation.

Conclusions: Our findings revealed a pivotal role of DAPK2 in thyroid carcinogenesis, being required for tumor growth and for resistance to TRAIL-induced apoptosis through autophagy-mediated I-κBα degradation. This result provides a novel target for the therapy of TC.
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http://dx.doi.org/10.21037/atm-21-2062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339828PMC
July 2021

Urinary albumin creatinine ratio associated with postoperative delirium in elderly patients undergoing elective non-cardiac surgery: A prospective observational study.

CNS Neurosci Ther 2021 Aug 20. Epub 2021 Aug 20.

Jiangsu Province Key Laboratory of Anesthesiology & NMPA Key Laboratory for Research and Evaluation of Narcotic and Psychotropic Drugs, Xuzhou Medical University, Xuzhou City, China.

Introduction: The blood-brain barrier (BBB) disruption contributes to postoperative delirium, but cost-effective and non-invasive assessment of its permeability is not practicable in the clinical settings. Urine albumin to creatinine ratio (UACR), reflecting systemic vascular endothelial dysfunction, may be a prognostic and predictive factor associated with postoperative delirium. The aim was to analyze the relationship between UACR and postoperative delirium in elderly patients undergoing elective non-cardiac surgery.

Materials And Methods: Through stratified random sampling, a cohort of 408 individuals aged 60 years and older scheduled for elective non-cardiac surgery were included between February and August 2019 in the single-center, prospective, observational study. The presence of delirium was assessed using the Confusion Assessment Method (CAM) or Confusion Assessment Method for the ICU (CAM-ICU) on the day of surgery, at 2 h after the surgery ending time and on the first 3 consecutive days with repeated twice-daily, with at least 6-h intervals between assessments. Urine samples were collected on one day before surgery, and 1st day and 3rd day after surgery. The primary outcome was the presence of postoperative delirium, and association of the level of UACR with postoperative delirium was evaluated with unadjusted/adjusted analyses and multivariable logistic regression.

Results: Postoperative delirium was observed in 26.75% (107 of 400) of patients within 3 days post-surgery. UACR-Pre (OR, 1.30; 95% CI, 1.14-1.49, p < 0.001), UACR-POD1 (OR, 1.20; 95% CI, 1.13-1.27, p < 0.001), and UACR-POD3 (OR, 1.14; 95% CI, 1.08-1.20, p < 0.001) between the delirium and non-delirium groups show a significant difference, even after adjusting for age, education levels, and other factors.

Conclusion: As the marker of endothelial dysfunction, the high perioperative UACR value may be linked to the postoperative delirium in elderly patients undergoing elective non-cardiac surgery.
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http://dx.doi.org/10.1111/cns.13717DOI Listing
August 2021

Long-term porosity and retreatability of oval-shaped canals obturated using two different methods with a novel tricalcium silicate sealer.

Clin Oral Investig 2021 Aug 14. Epub 2021 Aug 14.

Division of Endodontics, Department of Oral Biological & Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.

Objective: To evaluate the percentage volume of voids and gaps in oval-shaped canals obturated using two different methods with a tricalcium silicate-based sealer after short- or long-term storage. The long-term effect of storage on the efficiency of removing filling material was also investigated.

Materials And Methods: Forty premolar teeth with oval-shaped canals were instrumented to Reciproc R25 and obturated using single cone obturation (SCO) or warm vertical compaction (WVC) techniques with gutta-percha and HiFlow sealer. The specimens were stored at 100% humidity and 37°C for 2 weeks or 6 months and scanned using micro-computed tomography. Initial retreatment was performed up to a Reciproc R40, and the operating time was recorded. The residual material in the canal received a supplementary procedure using XP-endo Finisher R (XPFR) files. After each retreatment procedure, the specimens were rescanned.

Results: The percentage volume of voids and gaps in the SCO group was higher than that of the WVC group at both 2 weeks and 6 months (P < 0.05). The percentage volume of the filling material removed after initial retreatment and XPFR cleaning was significantly higher in the 6-month group than in the 2-week groups (P < 0.05). The proportion of the residual material decreased significantly when XPFR files were used, compared to the initial retreatment group (P < 0.05) in both storage times.

Conclusion: The efficiency of retreatment in the oval-shaped canal was closely related to the storage time rather than the filling technique using a tricalcium silicate sealer. The XPFR instrument proved effective in the removal of the remaining materials from the oval-shaped canal.

Clinical Relevance: Obturation of the oval-shaped canal with TSBS using the SCO technique in the coronal area needs to be optimized. The retreatment was less efficacious in freshly filled canals than aged filled canals.
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http://dx.doi.org/10.1007/s00784-021-04088-zDOI Listing
August 2021

Deorphanization of novel biogenic amine-gated ion channels identifies a new serotonin receptor for learning.

Curr Biol 2021 Aug 7. Epub 2021 Aug 7.

MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK; Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium. Electronic address:

Pentameric ligand-gated ion channels (LGICs) play conserved, critical roles in both excitatory and inhibitory synaptic transmission and can be activated by diverse neurochemical ligands. We have performed a characterization of orphan channels from the nematode C. elegans, identifying five new monoamine-gated LGICs with diverse functional properties and expression postsynaptic to aminergic neurons. These include polymodal anion channels activated by both dopamine and tyramine, which may mediate inhibitory transmission by both molecules in vivo. Intriguingly, we also find that a novel serotonin-gated cation channel, LGC-50, is essential for aversive olfactory learning of pathogenic bacteria, a process known to depend on serotonergic neurotransmission. Remarkably, the redistribution of LGC-50 to neuronal processes is modulated by olfactory conditioning, and lgc-50 point mutations that cause misregulation of receptor membrane expression interfere with olfactory learning. Thus, the intracellular trafficking and localization of these receptors at synapses may represent a molecular cornerstone of the learning mechanism.
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http://dx.doi.org/10.1016/j.cub.2021.07.036DOI Listing
August 2021

Sex-Related Characteristics, Quality of Care, and Short-Term Mortality Rates in Patient With Chronic Heart Failure.

Heart Lung Circ 2021 Aug 5. Epub 2021 Aug 5.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, Beijing, P.R. China; Cardiovascular Hospital, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China. Electronic address:

Background: The association of female sex with quality of care and short-term mortality rates in patients with chronic heart failure (CHF) remains controversial.

Method: We performed a retrospective study using data from 2,663 patients with CHF from nine hospitals in Beijing between January 2014 and December 2015. Multivariable logistic regression analyses were performed to investigate whether female sex was independently associated with quality of care and short-term mortality rates in Chinese patients with CHF.

Results: Compared to male patients, female patients (48%) were older and had a higher prevalence of comorbidities. Performance measures, including angiotensin-converting enzyme inhibitors or angiotensin receptor blockers, aldosterone receptor antagonists, and beta blocker use in patients with heart failure with reduced ejection fraction. warfarin therapy in those with atrial fibrillation, documentation of B-type natriuretic peptide/N-terminal pro-B-type natriuretic peptide, and left ventricular ejection fraction, showed no sex difference in multivariable analysis. Adjusted in-hospital mortality (1.7% vs 2.1%; adjusted odds ratio, 0.908; 95% confidence interval [CI], 0.448-1.842; p=0.789) and 30-day mortality (4.2% vs. 4.4%; adjusted hazard ratio, 0.908; 95% CI, 0.567-1.454; p=0.689) were comparable between the sexes.

Conclusion: Chinese female patients with CHF receive a similar quality of care and have similar short-term mortality rates as male patients.
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http://dx.doi.org/10.1016/j.hlc.2021.07.005DOI Listing
August 2021

A comparison of resin infiltration and microabrasion for postorthodontic white spot lesion.

Am J Orthod Dentofacial Orthop 2021 Jul 31. Epub 2021 Jul 31.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China. Electronic address:

Introduction: The objective of this research was to evaluate and compare the effectiveness of microabrasion and resin infiltration for white spot lesions (WSLs).

Methods: Patients with postorthodontic WSLs were enrolled and randomly assigned to the control, microabrasion, and resin-infiltration groups. Intraoral photographs were taken before and after (6 months later) treatment. WSL sizes were determined through ImageJ (Wayne Rasband, Kensington, Md). Integrated optical density (IOD) was determined for a WSL and its surrounding normal enamel through Image-Pro Plus (version 6.0; Media Cybernetics, Rockville, Md), and their differences of IOD were considered as the IOD surrogate for that WSL. The color change of WSL were measured through ΔE.

Results: A total of 27 eligible patients were enrolled; 9 subjects were assigned to each group, resulting in 56 teeth in the control group, 72 in the microabrasion group, and 58 in the resin-infiltration group. The ratios of WSL size (after/before) were similar between the microabrasion and resin-infiltration group (43.94 ± 0.03% vs 45.02 ± 0.03%; P = 0.96 > 0.05), but those of the 2 groups were significantly lower than those of the control group (92.15 ± 0.02%) (P <0.001). Moreover, the ratios of IOD (after/before) were significantly lower in the resin-infiltration group (22.94 ± 0.02%) than in the microabrasion (78.11 ± 0.03%) and control (83.79 ± 0.02%) (P <0.001) groups. The highest ΔE improvement was obtained by infiltration, but there was no significant difference between microabrasion and control group.

Conclusions: Resin infiltration and microabrasion are comparably effective in reducing the sizes of WSL, but resin infiltration enjoys an esthetic advantage over microabrasion.
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http://dx.doi.org/10.1016/j.ajodo.2020.04.039DOI Listing
July 2021

Preparation and antitumor activity of triphenylphosphine-based mitochondrial targeting polylactic acid nanoparticles loaded with 7-hydroxyl coumarin.

J Biomater Appl 2021 Aug 1:8853282211037030. Epub 2021 Aug 1.

Department of Pharmaceutics, School of Pharmacy, Hubei University of Science and Technology, Xianning, China.

Due to the low bioavailability and severe toxic side effects caused by the lack of selectivity of traditional chemotherapy drugs, the targeted delivery of chemotherapy drugs has become the key to tumor treatment. The activity and transmembrane potential of mitochondria in cancer cells were significantly higher than that of normal cells, making them a potential target for chemotherapeutic drug delivery. In this study, triphenylphosphine (TPP) based mitochondria targeting polylactic acid (PLLA) nanoparticles (TPP-PLLA NPs) were synthesized to improve the delivery efficiency of anticancer drugs. The carrier material was characterized by H NMR and FT-IR and 7-hydroxyl coumarin (7-HC) was successfully loaded into TPP-PLLA to form 7-HC/TPP-PLLA NPs. Further studies showed that TPP-PLLA NPs were primarily accumulated in the mitochondrial and 7-HC/TPP-PLLA NPs had higher antitumor activity. Taken together, our results indicated that TPP-PLLA NPs could be a promising mitochondria-targeted drug delivery system for cancer therapy.
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http://dx.doi.org/10.1177/08853282211037030DOI Listing
August 2021

Investigating SARS-CoV-2 persistent contamination in different indoor environments.

Environ Res 2021 Jul 28;202:111763. Epub 2021 Jul 28.

Tianjin Centers for Disease Control and Prevention, Tianjin, 300011, PR China.

Environmental contamination caused by COVID-19 patients could be a medium of transmission. Previous reports of SARS-CoV-2 in environmental surfaces were about short-term contamination. This study investigated SARS-CoV-2 RNA existence in room-temperature and low-temperature environments long after exposure (>28 days). A department store, where a COVID-19 outbreak was occurred in January 2020 (the epicenter of 43 COVID-19 patients), and a patient's apartment were included as room-temperature environments after being blocked for 57 days and 48 days, respectively. Seven cold storages and imported frozen foods inside were included as low-temperature environments (under -18 °C). Twenty food markets with potential contamination of imported frozen foods were also included to study the consecutive contamination. Information about temperature, relative humidity, and the number of days of environmental samples since the last exposure was collected and analyzed. In sum, 11,808 swab samples were collected before disinfection, of which 35 samples were positive. Persistent contamination of SARS-CoV-2 RNA was identified in the apartment (6/19), the department store (3/50), food packages in cold storages (23/1360), environmental surfaces of cold storages (2/345), and a package in the food market (1/10,034). Two positive samples were isolated from the bathroom of the apartment (66.7 %, 2/3), and doorknobs were proved with contamination in the apartment (40 %, 2/5) and cold storage (33.3 %, 1/3). The epidemiology information and environmental contamination results of an imported frozen food related COVID-19 case (138th COVID-19 patient in Tianjin) were analyzed. Based on the Ct values, the number of copies of two target genes was calculated by standard curves and linear regressions. In conclusion, SARS-CoV-2 RNA can be detected in room-temperature environments at least 57 days after the last exposure, much longer than previous reports. Based on the results of this study and previous studies, infectious SARS-CoV-2 could exist for at least 60 days on the surface of cold-chain food packages. Doorknobs and toilets (bathrooms) were important positions in COVID-19 control. High-risk populations of cold-chain-related logistic operations, such as porters, require strict prevention and high-level personal protection.
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http://dx.doi.org/10.1016/j.envres.2021.111763DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316642PMC
July 2021

Probiotic fermentation of Ganoderma lucidum fruiting body extracts promoted its immunostimulatory activity in mice with dexamethasone-induced immunosuppression.

Biomed Pharmacother 2021 Sep 10;141:111909. Epub 2021 Jul 10.

College of Basic Medical Science, Dalian Medical University, Dalian, China. Electronic address:

Ganoderma lucidum is a legendary traditional Chinese medicine with various bioactivities. This study was conducted (a) to explore the in vitro fermentation of the water extracts of G. lucidum fruiting body with Lactobacillus acidophilus and Bifidobacterium breve and (b) to investigate the effect of fermentation broth (GLFB) on dexamethasone (DEX)-induced immunosuppressed mice. Our results demonstrated that probiotic fermentation of G. lucidum fruiting body extracts underwent structural changing of major ganoderic acid components, such as ganoderic acid A (GA) into GC2, and this fermentation process involves changing of several metabolic pathways in the probiotic strains. GLFB could significantly improve the immunity, intestinal integrity, and gut microbiota dysbiosis in DEX-treated mice, and the immunostimulatory activity of GLFB was found closely related to its direct regulation on the expansion of CD4 T cells in Peyer's patches of mice. These data implied that probiotic fermentation of G. lucidum fruiting body extracts promoted its immunostimulatory activity via biotransformation of components such as GA. This research provides a theoretical support for the development and application of G. lucidum fermentation by probiotics.
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http://dx.doi.org/10.1016/j.biopha.2021.111909DOI Listing
September 2021

Fatty acid modified-antimicrobial peptide analogues with potent antimicrobial activity and topical therapeutic efficacy against Staphylococcus hyicus.

Appl Microbiol Biotechnol 2021 Aug 28;105(14-15):5845-5859. Epub 2021 Jul 28.

Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie St., Haidian District, Beijing, 100081, People's Republic of China.

There is an urgent need to explore new antimicrobial agents due to the looming threat of bacteria resistance. Bovine lactoferricin (LfcinB), as a multifunctional peptide, has the potential to be a new active drug in the future. In this study, it aims to investigate the effect of fatty acid conjugation on antimicrobial peptide activity and topical therapeutic efficacy in a mouse model infected with Staphylococcus hyicus. Both Lfcin4 and Lfcin5 were conjugated with the unsaturated fatty acid linoleic acid (18-C) at their N-terminus and modified by acylation at the C-terminus. The derived peptides of Lin-Lf4NH2 and Lin-Lf5NH2 showed better antibacterial activity (MICs of 3.27 to 6.64 μM) than their parent peptides (MICs of 1.83 to 59.57 μM). Lin-Lf4NH2 (63.2%, 5 min) and Lin-Lf5NH2 (35.8%, 5 min) could more rapidly penetrate bacterial membrane than Lf4NH2 (2.34%, 5 min) and Lf5NH2 (1.94%, 5 min), which further confirmed by the laser scanning confocal microscopy (LSCM). Electron microscopy observations showed Lin-Lf4NH2 and Lin-Lf5NH2 disrupted S. hyicus cell membranes and led to the leakage of contents. Furthermore, after treatment with Lin-Lf4NH2 and Lin-Lf5NH2, the abscess symptoms of mice were significantly alleviated; the recovery rate of abscesses scope of Lin-Lf4NH2 (73.25%) and Lin-Lf5NH2 (71.71%) were 38.8 and 37.9-fold higher than that of untreated group (1.89%), respectively, and superior to Lf4NH2 (46.87%) and Lf5NH2 (58.75%). They significantly reduced the bacterial load and the levels of the pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) and chemokine (MCP-1) in S. hyicus skin lesions. This study provides evidence that conjugation of a fatty acid to antimicrobial peptides can improve the activity and have potential for topical therapeutic of S. hyicus skin infections. KEY POINTS: • Lin-Lfcin4NH2/Lfcin5NH2 showed stronger antimicrobial activity than parent peptides. • Lin-Lfcin4NH2/Lfcin5NH2 had a more effective ability to destroy bacterial membranes. • Lin-Lfcin4NH2/Lfcin5NH2 showed a topically higher efficacy than parent peptides.
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http://dx.doi.org/10.1007/s00253-021-11454-0DOI Listing
August 2021

Comparison of prognosis and outcomes of catheter ablation versus drug therapy in patients with atrial fibrillation and stable coronary artery disease: A prospective propensity-score matched cohort study.

Clin Cardiol 2021 Jul 28. Epub 2021 Jul 28.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, China.

Background: Atrial fibrillation (AF) and stable coronary artery disease (SCAD) frequently coexist.

Hypothesis: To investigate the prognosis of catheter ablation versus drug therapy in patients with AF and SCAD.

Methods: In total, 25 512 patients with AF in the Chinese AF Registry between 2011 and 2019 were screened for SCAD. 815 patients with AF and SCAD underwent catheter ablation therapy were matched with patients by drug therapy in a 1:1 ratio. Primary end point was composite of thromboembolism, coronary events, major bleeding, and all-cause death. The secondary endpoints were each component of the primary endpoint and AF recurrence.

Results: Over a median follow-up of 45 ± 23 months, the patients in the catheter ablation group had a higher AF recurrence-free rate (53.50% vs. 18.41%, p < .01). In multivariate analysis, there was no significant difference between the strategy of catheter ablation and drug therapy in primary composite end point (adjusted HR 074, 95%CI 0.54-1.002, p = .0519). However, catheter ablation was associated with fewer all-cause death independently (adjusted HR 0.36, 95%CI 0.22-0.59, p < .01). In subgroup analysis, catheter ablation was an independent risk factor for all-cause death in the high-stroke risk group (adjusted HR 0.39, 95%CI 0.23-0.64, p < .01), not in the low-medium risk group (adjusted HR 0.17, 95%CI 0.01-2.04, p = .17).

Conclusions: In the patients with AF and SCAD, catheter ablation was not independently associated with the primary composite endpoint compared with drug therapy. However, catheter ablation was an independent protective factor of all-cause death.
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http://dx.doi.org/10.1002/clc.23699DOI Listing
July 2021

Synthesis of a pH-responsive nano-cellulose/sodium alginate/MOFs hydrogel and its application in the regulation of water and N-fertilizer.

Int J Biol Macromol 2021 Sep 24;187:262-271. Epub 2021 Jul 24.

Institute of Chemical Industry of Forestry Products, Chinese Academy of Forestry, Key Laboratory of Biomass Energy and Material, National Engineering Laboratory for Biomass Chemical Utilization, Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration, Nanjing 210042, Jiangsu Province, China. Electronic address:

In order to circumvent the water eutrophication caused by nitrogen loss in agriculture, slow-release and high-water containing fertilizers have captured much attention. Considering the unstable release of traditional slow-released fertilizers, novel strategies need to be designed to meet the steady release of fertilizers. Herein, by integrating cellulose-based hydrogel with MIL-100(Fe), a pH-sensitive Cellulose/MOFs hydrogel (CAM) with a high surface area (45.25 m/g) was devised. The volume changes and the water adsorption of the hydrogels were uncovered from pH 3 to pH 11, where the highest water adsorption (100 g/g) was achieved at pH 11. Besides, a pH-sensitive urea slow release fertilizer (U-CAM) was also designed. The urea release of the U-CAM at pH 11 was much slower than that of the U-CAM at pH 3, which indicated its potential application in arid regions. In parallel with a favorable water-holding capacity, the totally loss of the soil moisture loaded with U-CAM was slowed down by 18 days as compared with the pure soil. The positive effect of the U-CAM on the growth of wheat was indexed with the germination rate, number of tillers, photosynthetic rate and chlorophyll content of the crop, which verified their further application in irrigating farming.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.154DOI Listing
September 2021

Treatment of leiomyomatosis peritonealis disseminata with goserelin acetate: A case report and review of the literature.

World J Clin Cases 2021 Jul;9(19):5217-5225

Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Discipline Construction Research Center of China Hospital Development Institute, Shanghai Jiao Tong University, Shanghai 200011, China.

Background: Leiomyomatosis peritonealis disseminata (LPD) is a rare condition characterized by multiple pelvic and abdominal nodules, which are composed of smooth-muscle cells. To date, no more than 200 cases have been reported. The diagnosis of LPD is difficult and there are no guidelines on the treatment of LPD. Currently, surgical excision is the mainstay. However, hormone blockade therapy can be an alternative choice.

Case Summary: A 33-year-old female patient with abdominal discomfort and palpable abdominal masses was admitted to our hospital. She had undergone four surgeries related to uterine leiomyoma in the past 8 years. Computed tomography revealed multiple nodules scattered within the abdominal wall and peritoneal cavity. Her symptoms and the result of the core-needle biopsy were consistent with LPD. The patient refused surgery and was then treated with tamoxifen, ulipristal acetate (a selective progesterone receptor modulator), and goserelin acetate (a gonadotropin-releasing hormone agonist). Both tamoxifen and ulipristal acetate were not effective in controlling the disease progression. However, the patient achieved an excellent response when goserelin acetate was attempted with relieved syndromes and obvious shrinkage of nodules. The largest nodule showed a 25% decrease in the sum of the longest diameters from pretreatment to posttreatment. Up to now, 2 years have elapsed and the patient remains asymptomatic and there is no development of further nodules.

Conclusion: Goserelin acetate is effective for the management of LPD. The long-term use of goserelin acetate is thought to be safe and effective. Hormone blockade therapy can replace repeated surgical excision in recurrent patients.
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http://dx.doi.org/10.12998/wjcc.v9.i19.5217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283592PMC
July 2021

Fabrication of gelatin microspheres containing ammonium hydrogen carbonate for the tunable release of herbicide.

Biotechnol Lett 2021 Sep 18;43(9):1747-1755. Epub 2021 Jul 18.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.

The major challenge in utilizing pesticides lies in identifying the precise application that would improve the efficiency of these pesticides and decline their environmental and health hazards at the same time. Such application requires the development of specific formulations that enable controlled, stimuli-responsive release of the pesticides. Gelatin is a relatively cheap material characterized by temperature-sensitivity and abundant amino acid groups, which makes it suitable for the storage and controlled release of pesticides. In this study, gelatin microspheres were prepared by emulsion and cross-linking, then they were loaded with 2,4-dichlorophenoxyacetic acid sodium (2,4-D Na) as a model herbicide. To achieve temperature-tunable release of 2,4-D Na from the microspheres, NHHCO was added to the formulations at different concentrations. The prepared formulations were characterized by SEM, FTIR, and size distribution analyzes, and their drug loading capacities were determined. Based on bioassay experiments, the 2,4-D Na-NHHCO-loaded gelatin microspheres can effectively control the spread of dicotyledonous weeds. Therefore, the strategy proposed herein can be used to develop novel, effective herbicide formulations.
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http://dx.doi.org/10.1007/s10529-021-03163-5DOI Listing
September 2021

Algae biochar enhanced methanogenesis by enriching specific methanogens at low inoculation ratio during sludge anaerobic digestion.

Bioresour Technol 2021 Oct 4;338:125493. Epub 2021 Jul 4.

School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Key Laboratory of Anaerobic Biotechnology, Wuxi 214122, China; Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material, Suzhou 215011, China. Electronic address:

Carbon materials are promising in improving the performance of anaerobic digestion, however, interactive mechanisms between the carbon-based enhancement and operating parameters remained unclear. Using anaerobic digested sludge as inoculum, the effects of Taihu blue algae biochar (ABC) on methanogenesis at different inoculation ratios were investigated during sludge anaerobic digestion. Results showed that ABC enhanced methane productions at the lower inoculation ratios (4% and 1%, v/v), but not at the higher ratio (10%, v/v). Mechanism analysis demonstrated methanogenic improvements at the lower inoculation ratios were not owing to initial organic loading rate increments. Otherwise, ABC addition at the lower inoculation ratios were more favorable for the enrichment of Methanosarcina than the higher ratio, which might be benefit for methanogenesis through directed interspecies electron transfer. Thus, for the improvement of sludge anaerobic digestion, the microbial enrichments at different inoculation ratios would be more important than the merely biochar addition.
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http://dx.doi.org/10.1016/j.biortech.2021.125493DOI Listing
October 2021

Fractional Lévy stable motion with LRD for RUL and reliability analysis of li-ion battery.

ISA Trans 2021 Jul 9. Epub 2021 Jul 9.

Energy Department, Politecnico di Milano, Via La Masa 34/3, Milano, 20156, Italy; MINES ParisTech, PSL Research University, CRC, Sophia Antipolis, France. Electronic address:

The Remaining Useful Life (RUL) is important for reliability analysis of li-ion battery. Reliability of li-ion battery decreases with shortened the RUL. The RUL of li-ion battery can be revealed by the capacity change. The future change of the capacity is related to the current and the historical states, namely, the capacity change of li-ion battery has Long-Range Dependence (LRD). This article describes a RUL prediction method based on fractional order Lévy stable motion (fLsm), which solves the LRD was not obvious caused by the excessive difference of the integer-order model. First, the LRD of the fLsm is revealed by stability index and integral kernel function with Hurst parameter. Then, the fLsm is used as a diffusion term, which reflects the stochastic and LRD of the RUL degradation, to establish a degradation prediction model. The iterative form of the prediction model is established through the incremental distribution of the fLsm. Finally, the RUL is predicted by the Monte Carlo simulation and degradation prediction model. The predictive performance of the fLsm degradation model is verified by battery data in different operating environments. The reliability of li-ion battery is analyzed by the RUL.
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http://dx.doi.org/10.1016/j.isatra.2021.07.002DOI Listing
July 2021
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