Publications by authors named "He Jiang"

875 Publications

Upper Reference Limits for High-Sensitivity Cardiac Troponin T and N-Terminal Fragment of the Prohormone Brain Natriuretic Peptide in Patients With CKD.

Am J Kidney Dis 2021 Jul 19. Epub 2021 Jul 19.

University of Maryland School of Medicine, Department of Medicine.

Rationale & Objective: The utility of conventional upper reference limits (URL) for N-terminal pro brain natriuretic peptide (NT-proBNP) and high-sensitivity cardiac troponin T (hsTnT) in chronic kidney disease (CKD) remains debated. We analyzed the distribution of hsTnT and NT-proBNP in people with CKD in ambulatory settings to examine the diagnostic value of conventional URL in this population.

Study Design: Observational study.

Setting & Participants: We studied participants of the Chronic Renal Insufficiency Cohort with CKD and no self-reported history of cardiovascular disease (CVD).

Exposure: Estimated glomerular filtration rate (eGFR).

Outcomes: NT-proBNP and hsTnT at baseline.

Analytical Approach: We described the proportion of participants above the conventional URL for NT-proBNP (125 pg/ml) and hsTnT (14 ng/L) overall, and by eGFR. We then estimated 99 percentile URL for NT-proBNP and hsTnT. Using quantile regression of the 99 percentile, we modeled the association of eGFR with NT-proBNP and hsTnT.

Results: Among 2,312 CKD participants, 40% and 43% had levels of NT-proBNP and hsTnT above conventional URL, respectively. In those with eGFR<30 ml/min/1.73 m, 71% and 68% of participants had concentrations of NT-proBNP and hsTnT above conventional URL, respectively. Amongst all CKD participants, the 99 percentile for NT-proBNP was 3,592(95% CI: 2,470, 4,849) pg/mL and for hsTnT was 126(95% CI: 100, 144) ng/L. Each 15 ml/min/1.73 m decrement in eGFR was associated with a ∼40% higher threshold for the 99 percentile of NT-proBNP [1.43 (1.21, 1.69)] and hsTnT [1.45 (1.31, 1.60)].

Limitations: Study included ambulatory patients; we could not test the accuracy of upper reference limits of NT-proBNP and hsTnT in the acute care setting.

Conclusions: In this ambulatory CKD population with no self-reported history of CVD, a range of 40-88% of participants had concentrations of NT-proBNP and hsTnT above conventional URL, depending on eGFR strata. Developing eGFR-specific thresholds for these commonly-used cardiac biomarkers in the setting of CKD may improve their utility for evaluation of suspected heart failure and myocardial infarction.
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http://dx.doi.org/10.1053/j.ajkd.2021.06.017DOI Listing
July 2021

Corrigendum: Identification of a Potential miRNA-mRNA Regulatory Network Associated With the Prognosis of HBV-ACLF.

Front Mol Biosci 2021 29;8:705683. Epub 2021 Jun 29.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

[This corrects the article DOI: 10.3389/fmolb.2021.657631.].
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http://dx.doi.org/10.3389/fmolb.2021.705683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276013PMC
June 2021

Pangenomic Analysis of Strains Related to the Outbreak of Blackleg and Soft Rot of Potato in USA.

Plant Dis 2021 Jul 2. Epub 2021 Jul 2.

University of Maine, Plant, Soil & Environmental Sciences, 5735 Hitchner Hall, Room 174, Orono, Maine, United States, 04473.

has caused an outbreak of blackleg and soft rot of potato in the eastern half of the USA since 2015. To investigate genetic diversity of the pathogen, a comparative analysis was conducted on genomes of strains. Whole genomes of 16 strains from the USA outbreak were assembled and compared to 16 previously sequenced genomes of isolated from potato or carnation. Among the 32 strains, eight distinct clades were distinguished based on phylogenomic analysis. The outbreak strains were grouped into three clades, with the majority of the strains in clade I. Clade I strains were unique and homogeneous, suggesting a recent incursion of this strain into potato production from alternative hosts or environmental sources. Pangenome of the 32 strains contained 6693 genes, 3377 of which were core genes. By screening primary protein subunits associated with virulence from all USA strains, we found many virulence-related gene clusters, such as plant cell wall degrading enzyme genes, flagellar and chemotaxis related genes, two-component regulatory genes, and type I/II/III secretion system genes were highly conserved but type IV and type VI secretion system genes varied. The virulent clade I strains encoded two clusters of type IV secretion systems, while clade II and III strains encoded only one cluster. Clade I and II strains encoded one more VgrG/PAAR spike protein than clade III. Thus, we predicted that the presence of additional virulence-related genes may have enabled the unique clade I strain to become predominant source in the USA outbreak.
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http://dx.doi.org/10.1094/PDIS-03-21-0587-REDOI Listing
July 2021

Psychological Factors Affecting Risk Perception of COVID-19: Evidence from Peru and China.

Int J Environ Res Public Health 2021 06 17;18(12). Epub 2021 Jun 17.

Instituto de Investigación Ambiente Comportamiento y Sociedad, Cusco 08002, Peru.

COVID-19 has spread around the world, causing a global pandemic, and to date is impacting in various ways in both developed and developing countries. We know that the spread of this virus is through people's behavior despite the perceived risks. Risk perception plays an important role in decision-making to prevent infection. Using data from the online survey of participants in Peru and China ( = 1594), data were collected between 8 July 31 and August 2020. We found that levels of risk perception are relatively moderate, but higher in Peru compared to China. In both countries, anxiety, threat perception, self-confidence, and sex were found to be significant predictors of risk perception; however, trust in the information received by government and experts was significant only in Peru, whereas self-confidence had a significant negative effect only for China. Risk communication should be implemented through information programs aimed at reducing anxiety and improving self-confidence, taking into consideration gender differences. In addition, the information generated by the government should be based on empirical sources. Finally, the implications for effective risk communication and its impacts on the health field are discussed.
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http://dx.doi.org/10.3390/ijerph18126513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296494PMC
June 2021

Hyperlipidemia Influences the Accuracy of Glucometer-Measured Blood Glucose Concentrations in Genetically Diverse Mice.

Am J Med Sci 2021 Jun 29. Epub 2021 Jun 29.

Department of Radiology and Medical Imaging, University of Virginia, Charlottesville, VA, USA; Biochemistry & Molecular Genetics, University of Virginia, Charlottesville, VA, USA. Electronic address:

Background: Glucometers are widely used in animal research due to simplicity and ease of utilization, but their accuracy in blood glucose assessment for hyperlipidemic mice is unknown.

Methods: Here, we compared blood glucose levels measured by a glucometer with plasma glucose levels measured by a standard enzymatic assay for 325 genetically diverse F2 mice derived from LP and BALB/c (BALB) Apoe mice. Non-fasting glucose levels were measured before initiation of a Western diet and after 11 weeks on the diet.

Results: On chow diet, lab-measured plasma glucose levels were 279.5 ± 42.6 mg/dl (mean ± SD), while blood glucose values measured by glucometer were 138.7 ± 16.6 mg/dl. The two measures had no correlation (R = 0.006, p = 0.167). On the Western diet, plasma glucose levels rose to 351.1 ± 121.6 mg/dl, while glucometer-measured blood glucose fell to 128.7 ± 27.9 mg/dl. The two measures showed a moderate correlation (R = 0.111, p = 3.1E-9). Lab-measured plasma glucose showed strong positive correlations with plasma triglyceride and non-high-density lipoprotein cholesterol levels, while glucometer-measured blood glucose showed an inverse correlation with non-high-density lipoprotein levels on the chow diet.

Conclusions: Our results indicate that hyperlipidemia affects the accuracy of glucometers in measuring blood glucose levels of mice.
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http://dx.doi.org/10.1016/j.amjms.2021.06.014DOI Listing
June 2021

Designing an Electron-Deficient Pd/NiCoO Bifunctional Electrocatalyst with an Enhanced Hydrodechlorination Activity to Reduce the Consumption of Pd.

Environ Sci Technol 2021 Jul 1;55(14):10087-10096. Epub 2021 Jul 1.

College of Environment, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.

Reducing the Pd loading on electrodes is critical in the electrocatalytic hydrodechlorination (EHDC) of chlorinated organic compounds (COCs). The EHDC reaction of COCs on Pd involves three steps: H* formation, H* adsorption, and dechlorination. It has been established that the initial hydrogen evolution reaction (HER) occurs on Pd and the dechlorination steps occur on Pd. A strategy is proposed to design new electrodes by adding a reducible HER-active interlayer to replace Pd, fulfilling the responsibility of producing hydrogen, and to facilitate the formation of more Pd for following C-Cl bond cleavage. Keeping the atomic hydrogen adsorption energy on the Pd/interlayer similar to that on pure Pd is also necessary for H* adsorption as well as to maintain a high EHDC activity. For the first time, the NiCoO-interlayer-modified Pd/Ni-foam electrode was applied in the EHDC of COCs, which enhanced the EHDC efficiency to 100% within 90 min and reduced 88.6% of Pd consumption. The Pd/NiCoO/Ni-foam electrode with enhanced EHDC activity was also observed with almost 100% product selectivity and good stability. A synergistic mechanism is proposed for the enhanced EHDC activity on the Pd/NiCoO/Ni-foam. This work offers a simple and useful strategy to design robust electrocatalysts for the EHDC of COCs.
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http://dx.doi.org/10.1021/acs.est.1c01922DOI Listing
July 2021

Plasma Metabolomic Signatures of Healthy Dietary Patterns in the Chronic Renal Insufficiency Cohort (CRIC) Study.

J Nutr 2021 Jun 30. Epub 2021 Jun 30.

Welch Center for Prevention, Epidemiology, and Clinical Research, Johns Hopkins University, Baltimore, MD, USA.

Background: In individuals with chronic kidney disease (CKD), healthy dietary patterns are inversely associated with CKD progression. Metabolomics, an approach that measures many small molecules in biofluids, can identify biomarkers of healthy dietary patterns.

Objectives: We aimed to identify known metabolites associated with greater adherence to 4 healthy dietary patterns in CKD patients.

Methods: We examined associations between 486 known plasma metabolites and Healthy Eating Index (HEI)-2015, Alternative Healthy Eating Index (AHEI)-2010, the Dietary Approaches to Stop Hypertension (DASH) diet, and alternate Mediterranean diet (aMED) in 1056 participants (aged 21-74 y at baseline) in the Chronic Renal Insufficiency Cohort (CRIC) Study. Usual dietary intake was assessed using a semiquantitative FFQ. We conducted multivariable linear regression models to study associations between healthy dietary patterns and individual plasma metabolites, adjusting for sociodemographic characteristics, health behaviors, and clinical factors. We used principal component analysis to identify groups of metabolites associated with individual food components within healthy dietary patterns.

Results: After Bonferroni correction, we identified 266 statistically significant diet-metabolite associations (HEI: n = 60; AHEI: n = 78; DASH: n = 77; aMED: n = 51); 78 metabolites were associated with >1 dietary pattern. Lipids with a longer acyl chain length and double bonds (unsaturated) were positively associated with all 4 dietary patterns. A metabolite pattern low in saturated diacylglycerols and triacylglycerols, and a pattern high in unsaturated triacylglycerols was positively associated with intake of healthy food components. Plasmalogens were negatively associated with the consumption of nuts and legumes and healthy fat, and positively associated with the intake of red and processed meat.

Conclusions: We identified many metabolites associated with healthy dietary patterns, indicative of food consumption. If replicated, these metabolites may be considered biomarkers of healthy dietary patterns in patients with CKD.
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http://dx.doi.org/10.1093/jn/nxab203DOI Listing
June 2021

Association of fine particulate matter air pollution and its constituents with lung function: The China Pulmonary Health study.

Environ Int 2021 Jun 26;156:106707. Epub 2021 Jun 26.

Department of Respiratory and Critical Care Medicine, Beijing Hospital, Beijing, China; National Center of Gerontology, Beijing, China.

The associations of long-term exposure to various constituents of fine particulate matter (≤2.5 μm in aerodynamic diameter, PM) air pollution with lung function were not clearly elucidated in developing countries. The aim was to evaluate the associations of long-term exposure to main constituents of PM with lung function in China. This is a nationwide, cross-sectional analysis among 50,991 study participants from the China Pulmonary Health study. Multivariable linear regression models were used to obtain differences of forced expiratory volume in 1 s (FEV), forced vital capacity (FVC), FEV/FVC, peak expiratory flow (PEF), and forced expiratory flow at 25-75% of exhaled FVC (FEF) associated with an interquartile range (IQR) change of PM or its constituents. Residential annual PM levels varied from 26 μg/m to 92 μg/m (average: 53 μg/m). An IQR increase of PM concentrations was associated with lower FEV (19.82 mL, 95% CI: 11.30-28.33), FVC (17.45 mL, 95% CI: 7.16-27.74), PEF (86.64 mL/s, 95% CI: 59.77-113.52), and FEF (31.93 mL/s, 95% CI: 16.64-47.22). Black carbon, organic matter, ammonium, sulfate, and nitrate were negatively associated with most lung function indicators, with organic matter and nitrate showing consistently larger magnitude of associations than PM mass. This large-scale study provides first-hand epidemiological evidence that long-term exposure to ambient PM and some constituents, especially organic matter and nitrate, were associated with lower large- and small- airway function.
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http://dx.doi.org/10.1016/j.envint.2021.106707DOI Listing
June 2021

Plasma Kidney Injury Molecule 1 in CKD: Findings From the Boston Kidney Biopsy Cohort and CRIC Studies.

Am J Kidney Dis 2021 Jun 24. Epub 2021 Jun 24.

Section of Nephrology, Department of Medicine, Boston University School of Medicine, Boston Medical Center, Boston, MA; Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA. Electronic address:

Rationale & Objective: Plasma kidney injury molecule-1 (KIM-1) is a sensitive marker of proximal tubule injury, but its association with risks of adverse clinical outcomes across a spectrum of kidney diseases is unknown.

Study Design: Prospective, observational cohort study.

Setting & Participants: 524 individuals undergoing clinically indicated native kidney biopsy with biopsy specimens adjudicated for semiquantitative scores of histopathology by two kidney pathologists enrolled into the Boston Kidney Biopsy Cohort (BKBC) Study and 3,800 individuals with common forms of chronic kidney disease (CKD) enrolled into the Chronic Renal Insufficiency Cohort (CRIC) Study.

Exposure: Histopathologic lesions and clinicopathologic diagnosis in cross-sectional analyses, baseline plasma KIM-1 in prospective analyses.

Outcomes: Baseline plasma KIM-1 in cross-sectional analyses, kidney failure (defined as initiation of kidney replacement therapy) and death in prospective analyses.

Analytical Approach: Multivariable-adjusted linear regression models tested associations of plasma KIM-1 with histopathologic lesions and clinicopathologic diagnoses. Cox proportional hazards models tested associations of plasma KIM-1 with future kidney failure and death.

Results: In the BKBC Study, higher plasma KIM-levels were associated with more severe acute tubular injury, tubulointerstitial inflammation, and more severe mesangial expansion after multivariable adjustment. Participants with diabetic nephropathy, glomerulopathies, and tubulointerstitial disease had significantly higher plasma KIM-1 levels after multivariable adjustment. In the BKBC Study, 124 participants progressed to kidney failure and 85 participants died during a median follow-up time of 5 years. In the CRIC Study, 1153 participants progressed to kidney failure and 1356 participants died during a median follow-up time of 11.5 years. In both cohorts, each doubling of plasma KIM-1 was associated with an increased risk of kidney failure after multivariable adjustment (BKBC: HR 1.19, 95% CI 1.03 to 1.38 and CRIC: HR 1.10, 95% CI 1.06 to 1.15). There was no statistically significant association of plasma KIM-1 with death in either cohort.

Limitations: Generalizability and unmeasured confounding.

Conclusions: Plasma KIM-1 is associated with underlying tubulointerstitial and mesangial lesions and progression to kidney failure in two cohort studies of individuals with kidney diseases.
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http://dx.doi.org/10.1053/j.ajkd.2021.05.013DOI Listing
June 2021

(Hetero)arene-fused boroles: a broad spectrum of applications.

Chem Sci 2020 Nov 24;12(1):128-147. Epub 2020 Nov 24.

Institute for Inorganic Chemistry and Institute for Sustainable Chemistry & Catalysis with Boron (ICB), Julius-Maximilians-Universität Würzburg Am Hubland 97074 Würzburg Germany

(Hetero)arene-fused boroles are a class of compounds containing a 5-membered boron diene-ring. Based on their molecular framework, the (hetero)arene-fused boroles can be considered as boron-doped polycyclic antiaromatic hydrocarbons and are thus of great interest. Due to the vacant p orbital on the 3-coordinate boron atom, the antiaromaticity and strain of the 5-membered borole ring, (hetero)arene-fused boroles possess strong electron accepting abilities and Lewis acidity. By functionalization, they can be tuned to optimize different properties for specific applications. Herein, we summarize synthetic methodologies, different strategies for their functionalization, and applications of (hetero)arene-fused boroles.
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http://dx.doi.org/10.1039/d0sc05676fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178973PMC
November 2020

Plasma choline and betaine and risks of cardiovascular events and recurrent stroke after ischemic stroke.

Am J Clin Nutr 2021 Jun 22. Epub 2021 Jun 22.

Department of Epidemiology, School of Public Health and Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Medical College of Soochow University, Suzhou, China.

Background: Choline and betaine have been suggested to play a pivotal role in neurotransmitter synthesis, cell membrane integrity, and methyl-group metabolism, exerting neuroprotective effects in patients with various neurological disorders. However, population-based evidence on choline and betaine with subsequent cardiovascular events after stroke is rare.

Objectives: We aimed to prospectively investigate the relationships of circulating choline and betaine with cardiovascular events and recurrent stroke in patients with ischemic stroke.

Methods: We performed a nested case-control study within the China Antihypertensive Trial in Acute Ischemic Stroke. A total of 323 cardiovascular events (including 264 recurrent strokes) and 323 controls (free of recurrent cardiovascular events) matched for age (±1 y), sex, and treatment group were included. The primary endpoint was a composite of cardiovascular events after ischemic stroke. Plasma choline and betaine were measured at baseline by ultra-high-performance LC-MS/MS. Conditional logistic regression models were applied, and discrimination, reclassification, and calibration of models with choline pathway metabolites were evaluated.

Results: Plasma choline and betaine were inversely associated with cardiovascular events and recurrent stroke after ischemic stroke. Specifically, in fully adjusted models, each additional SD of choline and betaine was associated with 35% (95% CI: 20%-48%) and 30% (95% CI: 14%-43%) decreased risks of subsequent cardiovascular events, respectively, and 34% (95% CI: 16%-48%) and 29% (95% CI: 12%-43%) decreased risks of recurrent stroke, respectively. In addition, both choline and betaine offered substantial risk discrimination and reclassification improvement for cardiovascular events and recurrent stroke beyond traditional risk factors, as evidenced by an increase in C statistics, the net reclassification index, and integrated discrimination improvement.

Conclusions: Plasma choline pathway metabolites, including choline and betaine, were associated with decreased risks of cardiovascular events and recurrent stroke and provided incremental value in risk discrimination and stratification in patients with ischemic stroke. This nested case-control study was based on the China Antihypertensive Trial in Acute Ischemic Stroke, which is registered at clinicaltrials.gov as NCT01840072.
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http://dx.doi.org/10.1093/ajcn/nqab199DOI Listing
June 2021

The engineered expression of secreted HSPB5-Fc in CHO cells exhibits cytoprotection in vitro.

BMC Biotechnol 2021 Jun 14;21(1):39. Epub 2021 Jun 14.

Joint National Laboratory for Antibody Drug Engineering, The First Affiliated Hospital of Henan University, School of Basic Medical Sciences, Henan University, Jin-Ming Road, Kaifeng, 475004, China.

Background: HSPB5 is an ATP-independent molecular chaperone that is induced by heat shock or other proteotoxic stresses. HSPB5 is cytoprotective against stress both intracellularly and extracellularly. It acts as a potential therapeutic candidate in ischemia-reperfusion and neurodegenerative diseases.

Results: In this paper, we constructed a recombinant plasmid that expresses and extracellularly secrets a HSPB5-Fc fusion protein (sHSPB5-Fc) at 0.42 μg/ml in CHO-K1 cells. This sHSPB5-Fc protein contains a Fc-tag at the C-terminal extension of HSPB5, facilitating protein-affinity purification. Our study shows that sHSPB5-Fc inhibits heat-induced aggregation of citrate synthase in a time and dose dependent manner in vitro. Administration of sHSPB5-Fc protects lens epithelial cells against cisplatin- or UVB-induced cell apoptosis. It also decreases GFP-Htt-Q74 insolubility, and reduces the size and cytotoxicity of GFP-Htt-Q74 aggregates in PC-12 cells.

Conclusion: This recombinant sHSPB5-Fc exhibits chaperone activity to protect cells against proteotoxicity.
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http://dx.doi.org/10.1186/s12896-021-00700-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204567PMC
June 2021

Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration.

J Immunol Res 2021 24;2021:9995869. Epub 2021 May 24.

Xiangya Cancer Center, Xiangya Hospital, Central South University, Changsha 410008, China.

The majority of diffuse large B-cell lymphoma (DLBCL) patients develop relapsed or refractory disease after standard ruxolitinib, cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) chemotherapy, which is partly related to a dysregulated tumor immune microenvironment. However, how the infiltration of immune cells is appropriately regulated is poorly understood. Herein, we show that the E3 ubiquitin ligase Trim35 is expressed at low levels in human DLBCL tissues. We also show that overexpression of Trim35 suppresses DLBCL cell proliferation and correlates with inferior survival in DLBCL patients. Our mechanistic study shows that Trim35 functions as an E3 ligase to mediate the ubiquitination and degradation of CLOCK, a key regulator of circadian rhythmicity. High expression of Trim35 correlates with NK cell infiltration in DLBCL, partly due to the degradation of CLOCK. Consistently, patients with high expression of CLOCK show poor overall survival. Overall, these findings suggest that Trim35 suppresses the progression of DLBCL by modulating the tumor immune microenvironment, indicating that it may be a promising diagnostic and prognostic biomarker in DLBCL.
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http://dx.doi.org/10.1155/2021/9995869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166485PMC
May 2021

Structural insights into two distinct nanobodies recognizing the same epitope of green fluorescent protein.

Biochem Biophys Res Commun 2021 Aug 4;565:57-63. Epub 2021 Jun 4.

Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai, 200438, China. Electronic address:

Green fluorescent protein (GFP) and its derivatives are widely used in biomedical research, and the manipulation of GFP-tagged proteins by GFP-specific binders is highly desired. However, structural information on how these binders bind with GFP is still lacking. In this study, we determined the crystal structure of the nanobody Nb2 complexed with superfolder GFP (sfGFP) at a resolution of 2.2 Å. Interestingly, although the complementarity-determining regions (CDRs) of Nb2 and LaG16 sequences were only 29.7% identical, they both bound to the same epitope of GFP and existed in the same orientation. Structural analysis indicated that they achieved similar binding characteristics through different mechanisms. We further verified the kinetics and thermodynamics of binding by biolayer interferometry (BLI) and isothermal titration calorimetry (ITC). Nb2 showed a slightly higher binding affinity for sfGFP than LaG16. The stability of GFP-specific nanobodies was verified by nano differential scanning fluorimetry (nanoDSF). Nb2 exhibited the highest melting temperature (Tm); thus, Nb2 is a promising GFP nanobody candidate for use in applications requiring harsh testing conditions. We also compared the binding sites of available GFP nanobodies and showed that some of them can simultaneously bind with GFP and assemble into multifunctional complexes to manipulate GFP-tagged target proteins. Our results provide atomic-scale binding information for Nb2-sfGFP, which is important for the further development of GFP-nanobody based fusion protein manipulation techniques.
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http://dx.doi.org/10.1016/j.bbrc.2021.05.089DOI Listing
August 2021

Early Contributors to Healthy Arterial Aging Versus Premature Atherosclerosis in Young Adults: The Bogalusa Heart Study.

J Am Heart Assoc 2021 Jun 5;10(12):e020774. Epub 2021 Jun 5.

Department of Medicine Tulane University School of Medicine New Orleans LA.

Background Early identification of healthy arterial aging versus premature atherosclerosis is important for optimal atherosclerotic cardiovascular disease risk stratification and prevention. We sought to identify predictors for the long-term absence of carotid plaque among young adults. Methods and Results We included 508 participants from the Bogalusa Heart Study without clinical atherosclerotic cardiovascular disease who were free of carotid plaque at baseline (2001-2002) and underwent ultrasound imaging at follow-up (2013-2016). Modified Poisson regression estimated the persistent absence of plaque over 12.8 years. Participants were on average age 36.2 years at baseline, 64% were women, and 29% were Black. Although nearly all participants (97%) had a 10-year atherosclerotic cardiovascular disease risk <7.5%, there were 162 people (32%) who developed premature atherosclerosis. Aside from younger age (risk ratio [RR], 1.21; 95% CI, 1.07-1.36, per 10 years) and a total cholesterol/high-density lipoprotein cholesterol ratio <3.5 (RR, 1.15; 95% CI, 1.01-1.30), normal values of traditional risk factors did not predict long-term absence of plaque. Independent from traditional markers including glomerular filtration rate, serum calcium-phosphate product (RR, 1.07; 95% CI, 1.01-1.14, per 1-SD lower), phosphate (RR, 1.15; 95% CI, 1.03-1.29, per 1 mg/dL lower), and dietary sodium <2300 mg/day (RR, 1.20; 95% CI, 1.02-1.41) were significantly associated with the non-development of plaque. Conclusions Nearly one third of young adults with a low burden of traditional risk factors developed premature atherosclerosis. Beyond younger age and an ideal lipoprotein profile, lower calcium-phosphate homeostasis and low sodium intake were associated with long-term absence of carotid plaque. These results suggest that dietary and intrinsic minerals are early contributors to the development of arterial aging phenotypes.
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http://dx.doi.org/10.1161/JAHA.121.020774DOI Listing
June 2021

Prognostic and Clinicopathological Value of Human Leukocyte Antigen G in Gastrointestinal Cancers: A Meta-Analysis.

Front Oncol 2021 12;11:642902. Epub 2021 May 12.

Department of Nutrition, School of Medicine, Jinan University, Guangzhou, China.

Background: The prognostic value of human leukocyte antigen G (HLA-G) expression in gastrointestinal (GI) cancers remains controversial. Thus, this meta-analysis aimed to summarize available evidence from case-control or cohort studies that evaluated this association.

Methods: The PubMed, EMBASE, Cochrane Library, and Web of Science databases were searched to identify relevant studies written in English published up to April 1, 2021, and with no initial date. Furthermore, the Google Scholar and Google databases were also searched manually for gray literature. The protocol for this meta-analysis was registered at PROSPERO (CRD42020213411). Pooled hazard ratios (HRs) or odds ratios (ORs) and 95% confidence intervals (CIs) were estimated for end points using fixed- and random-effects statistical models to account for heterogeneity. Publication bias was evaluated using a funnel plot, Begg's and Egger's tests, and the "trim and fill" method.

Results: A total of 30 eligible articles with 5737 unique patients, including 12 studies on colorectal cancer (CRC), 6 on gastric cancer (GC), 5 on esophageal cancer (ESCC), 5 on hepatocellular carcinoma (HCC), and 2 on pancreatic adenocarcinoma (PC), were retrieved. Both univariate (HR = 2.01, 95% CI: 1.48 ~ 2.72) and multivariate (HR = 2.69, 95% CI: 2.03 ~ 3.55) analyses revealed that HLA-G expression was significantly correlated with poor overall survival (OS), regardless of the cancer type or antibody used. Subgroup analysis stratified by antibody showed that the 4H84 ( = 45.8%, = 0.101) antibodies could be trustworthy and reliable for detecting HLA-G expression in GI cancers. In addition, HLA-G expression was found to be correlated with adverse clinicopathological parameters such as clinical stage, nodal status, metastasis, and histological grade but not tumor status.

Conclusion: Elevated HLA-G expression indicates a poor prognosis for GI cancer patients, and screening for this marker could allow for the early diagnosis and treatment of GI cancers to improve survival rates.
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http://dx.doi.org/10.3389/fonc.2021.642902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149900PMC
May 2021

Time-Updated Changes in Estimated GFR and Proteinuria and Major Adverse Cardiac Events: Findings from the Chronic Renal Insufficiency Cohort (CRIC) Study.

Am J Kidney Dis 2021 May 27. Epub 2021 May 27.

Department of Biostatistics, Epidemiology, and Informatics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA.

Rationale And Objective: Evaluating repeated measures of estimated glomerular filtration rate (eGFR) and urine protein-to-creatinine ratio (UPCR) over time may enhance our ability to understand the association between changes in kidney parameters and cardiovascular disease risk.

Study Design: Prospective cohort study.

Setting And Participants: Annual visit data from 2,438 participants in the Chronic Renal Insufficiency Cohort.

Exposures: Average and slope of eGFR and UPCR in time-updated, one-year exposure windows.

Outcomes: Incident heart failure, atherosclerotic cardiovascular disease events, death, and a composite of incident heart failure, atherosclerotic cardiovascular disease events, and death.

Analytic Approach: A landmark analysis, a dynamic approach to survival modeling that leverages longitudinal, iterative profiles of laboratory and clinical information to assess the time-updated three-year risk of adverse cardiovascular outcomes.

Results: Adjusting for baseline and time-updated covariates, every standard deviation lower mean eGFR (19 mL/min/1.73m) and declining slope of eGFR (8 mL/min/1.73m/year) were independently associated with a higher risk of heart failure (mean eGFR hazard ratio [HR] 1.82, 95% confidence interval 1.39-2.44; slope HR 1.28, 1.12-1.45) and the composite outcome (mean HR 1.32, 1.11-1.54; slope HR 1.11, 1.03-1.20). Every standard deviation higher mean UPCR (136 mg/g) and rising UPCR (240 mg/g/year) were also independently associated with a higher risk of heart failure (mean HR 1.58, 1.28-1.97; slope HR 1.20, 1.10-1.29) and the composite outcome (mean HR 1.33, 1.17-1.50; slope HR 1.12, 1.06-1.18).

Limitations: Limited generalizability of annual eGFR and UPCR measurements; several biomarkers for cardiovascular disease risk were not available annually.

Conclusions: Using the landmark approach to account for time-updated patterns of kidney function, average and slope of eGFR and proteinuria were independently associated with three-year cardiovascular risk. Short-term changes in kidney function provide information about cardiovascular risk incremental to level of kidney function, representing possible opportunities for more effective management of patients with chronic kidney disease.
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http://dx.doi.org/10.1053/j.ajkd.2021.03.021DOI Listing
May 2021

Heating events drive the seasonal patterns of volatile organic compounds in a typical semi-arid city.

Sci Total Environ 2021 Sep 19;788:147781. Epub 2021 May 19.

School of Ecology and Environment, Inner Mongolia University, 010021 Hohhot, China; Institute of Environmental Geology, Inner Mongolia University, Hohhot 010021, China. Electronic address:

The emission characteristics, source apportionment and chemical behavior of volatile organic compounds (VOCs) are important for strategy-making on ozone (O) and fine particulate matter (PM) control. Based on the continuous observation during four seasons, the seasonal characteristics, chemical reactivity and source apportionment of 116 VOCs species were studied in a typical semi-arid city with no relevant research. The results showed that the annual average concentrations of total volatile organic compounds (TVOCs) in Hohhot was 44.67 ± 46.59 ppbv with the predominant of alkanes and oxygenated volatile organic compounds (OVOCs). The sharp increment of TVOCs were explained by the elevating OVOCs and alkanes in autumn, while alkanes and alkenes in winter. The levels of alkenes presented negative and positive correlations with solar radiation and PM, respectively. The mixing ratios accounted for 30% (alkanes) and 23% (alkenes and aromatics) of the TVOCs, respectively; while their ozone formation potential (OFP) ~15% and nearly 50% (even 75% in winter), respectively, indicating that the OFP of different VOCs species depends not only on their concentrations but more importantly on their chemical activity in atmosphere. According to the seasonal source apportionment, both the high levels of short-chain alkanes, alkenes and aromatics and the increasing coal sales volume suggested that the combustion sources were the predominant in heating seasons, while solvent uses was extracted as the most predominant during non-heating seasons. In non-heating seasons, the biogenic emission sources, ranking as the second contributor, were significantly higher than heating seasons. Isoprene was the most active biogenic VOCs species, bagging test results showed that deciduous trees were the predominant contributors for isoprene (~99%), while coniferous trees and shrub for monoterpenes (>95%). It will be helpful for understanding the characteristics of VOCs in Chinese national key development areas and informing policy to control semi-arid regional VOCs air pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147781DOI Listing
September 2021

Reduction in total and major cause-specific mortality from tobacco smoking cessation: a pooled analysis of 16 population-based cohort studies in Asia.

Int J Epidemiol 2021 Feb 5. Epub 2021 Feb 5.

Division of Epidemiology, Department of Medicine, Vanderbilt Epidemiology Center, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN, USA.

Background: Little is known about the time course of mortality reduction following smoking cessation in Asians who have smoking behaviours distinct from their Western counterparts. We evaluated the level of reduction in all-cause, cardiovascular disease (CVD) and lung cancer mortality by years since quitting smoking, in Asia.

Methods: Using Cox regression, we analysed individual participant data (n = 709 151) from 16 prospective cohorts conducted in China, Japan, Korea/Singapore, and India/Bangladesh, separately by cohorts. Cohort-specific hazard ratios (HRs) were combined using a random-effects meta-analysis.

Results: During a mean follow-up of 12.0 years, 108 287 deaths were ascertained-35 658 from CVD and 7546 from lung cancer. Among Asian men, a dose-response relationship of risk reduction in deaths from all causes, CVD and lung cancer was observed with an increase in years after smoking cessation. Compared with never smokers, however, all-cause and CVD mortality among former smokers remained elevated 10-14 years after quitting [multivariable-adjusted HR (95% confidence interval (CI) = 1.25 (1.13-1.37) and 1.20 (1.02-1.41), respectively]. Lung cancer mortality stayed almost 2-fold higher than among never smokers 15-19 years after smoking cessation [1.97 (1.41-2.73)], particularly among former heavy smokers [2.62 (1.71-4.00)]. Women who quitted for ≥5 years retained a significantly elevated mortality from all causes, CVD and lung cancer. Overall patterns of the cessation-mortality associations were similar across countries.

Conclusions: Our findings suggest that adverse effects of tobacco smoking persist for an extended time period, even for more than two decades, which is beyond the time windows defined in current clinical guidelines for risk assessment of lung cancer and CVD.
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http://dx.doi.org/10.1093/ije/dyab087DOI Listing
February 2021

Identification of a Potential miRNA-mRNA Regulatory Network Associated With the Prognosis of HBV-ACLF.

Front Mol Biosci 2021 28;8:657631. Epub 2021 Apr 28.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Background: Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a life-threatening disease with a high mortality rate; the systemic inflammatory response plays a vital role in disease progression. We aimed to determine if a miRNA-mRNA co-regulatory network exists in the peripheral blood mononuclear cells (PBMCs) of HBV-ACLF patients, which might be important for prognosis.

Methods: In patients with HBV-ACLF meeting COSSH-ACLF criteria, age, liver cirrhosis and INR were independent risk factors for 28-day and 90-day poor prognosis. COSSH-ACLFs was a superior prognostic model. mir-6840-3p-JADE2 may promote the progression of ACLF and lead to poor prognosis. Meanwhile, mir-6840-3p and mir-6861-3p can be used as markers of short-term poor prognosis. Finally, ALSS treatment is not only the blood material exchange of patients, but also changes part of the immune state of patients. Among them, cytokine cytokine receptor interaction may play an important role in determining the therapeutic effect.

Methods: Transcriptome-wide microRNA (miRNA) and mRNA microarrays were used to define the miRNA and mRNA expression profiles of the PBMCs of HBV-ACLF patients in a discovery cohort. The targets of the miRNAs were predicted. We built a miRNA-mRNA regulatory network through bioinformatics analysis, and used quantitative real-time polymerase chain reaction (qRT-PCR) to assess the importance of candidate miRNAs and mRNAs. We also assessed the direct and transcriptional regulatory effects of miRNAs on target mRNAs using a dual-luciferase reporter assay.

Results: The miRNA/mRNA PBMC expression profiles of the discovery cohort, of whom eight survived and eight died, revealed a prognostic interactive network involving 38 miRNAs and 313 mRNAs; this was constructed by identifying the target genes of the miRNAs. We validated the expression data in another cohort, of whom 43 survived and 35 died; miR-6840-3p, miR-6861-3p, JADE2, and NR3C2 were of particular interest. The levels of miR-6840-3p and miR-6861-3p were significantly increased in the PBMCs of the patients who died, and thus predicted prognosis (areas under the curve values = 0.665 and 0.700, respectively). The dual-luciferase reporter assay indicated that miR-6840-3p directly targeted JADE2.

Conclusion: We identified a prognostic miRNA-mRNA co-regulatory network in the PBMCs of HBV-ACLF patients. miR-6840-3p-JADE2 is a potential miRNA-mRNA pair contributing to a poor prognosis.
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http://dx.doi.org/10.3389/fmolb.2021.657631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113841PMC
April 2021

Advances in Genomics Research of Blood Pressure Responses to Dietary Sodium and Potassium Intakes.

Hypertension 2021 Jul 17;78(1):4-15. Epub 2021 May 17.

Department of Epidemiology, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA (L.A.B., Z.H., C.F., A.C.R., J.H., T.N.K.).

More than half of US adults have hypertension by 40 years of age and a subsequent increase in atherosclerotic cardiovascular disease risk. Dietary sodium and potassium are intricately linked to the pathophysiology of hypertension. However, blood pressure responses to dietary sodium and potassium, phenomena known as salt and potassium sensitivity of blood pressure, respectively, are heterogenous and normally distributed in the general population. Like blood pressure, salt and potassium sensitivity are complex phenotypes, and previous research has shown that up to 75% of individuals experience a blood pressure change in response to such dietary minerals. Previous research has also implicated both high salt sensitivity and low salt sensitivity (or salt resistance) of blood pressure to an increased risk of hypertension and potentially atherosclerotic cardiovascular disease risk. Given the clinical challenges required to accurately measure the sodium and potassium response phenotypes, genomic characterization of these traits has become of interest for hypertension prevention initiatives on both the individual and population levels. Here, we review advances in human genomics research of blood pressure responses to dietary sodium and potassium by focusing on 3 main areas, including the phenotypic characterization of salt sensitivity and resistance, clinical challenges in diagnosing such phenotypes, and the genomic mechanisms that may help to explain salt and potassium sensitivity and resistance. Through this process, we hope to further underline the value of leveraging genomics and broader multiomics for characterizing the blood pressure response to sodium and potassium to improve precision in lifestyle approaches for primordial and primary atherosclerotic cardiovascular disease prevention.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.121.16509DOI Listing
July 2021

Comparative effectiveness of team-based care with a clinical decision support system versus team-based care alone on cardiovascular risk reduction among patients with diabetes: Rationale and design of the D4C trial.

Am Heart J 2021 Aug 4;238:45-58. Epub 2021 May 4.

Department of Endocrinology and Diabetes, Xiamen Diabetes Institute, and Xiamen Clinical Medical Center for Endocrine and Metabolic Diseases, the First Affiliated Hospital of Xiamen University, Xiamen, China; Fujian Province Key Laboratory of Diabetes Translational Medicine, Xiamen, China.. Electronic address:

Background: Diabetes has become a major public health challenge worldwide, especially in low- and middle-income countries (LMICs). Uncontrolled hyperglycemia, hypertension, and dyslipidemia major risk factors for all-cause mortality and cardiovascular disease (CVD) are common in patients with diabetes in China. We propose to compare the effectiveness of team-based care plus a clinical decision support system (CDSS) with team-based care alone on glycemic, blood pressure (BP), and lipid control, and clinical CVD reduction among patients with type-2 diabetes and at high risk for CVD.

Methods: The Diabetes Complication Control in Community Clinics (D4C) study is a cluster-randomized trial conducted among 38 community health centers in Xiamen City, China. Nineteen clinics have been randomly assigned to team-based care plus CDSS and 19 to team-based care alone. Team-based care includes primary care providers, health coaches, and diabetes specialists working collaboratively with patients to achieve shared treatment goals for CVD risk factor reduction. The CDSS integrates guideline-based treatment algorithms for glycemic, BP, and lipid control, along with a patient's medical history and insurance policy, to recommend treatment and follow-up plans. In phase 1, the co-primary outcomes are mean reduction in glycated hemoglobin (HbA1c), systolic BP (SBP), and low-density lipoprotein (LDL)-cholesterol over 18 months, and the proportion of patients with controlled HbA1c, SBP, and LDL-cholesterol at 18 months' between the 2 comparison groups. In phase 2, the primary outcome is the difference in major CVD incidence (non-fatal stroke, non-fatal myocardial infarction, hospitalized heart failure, and CVD mortality) between the 2 comparison groups. Mean reduction in HbA1c, SBP, and LDL-cholesterol levels will be simultaneously modeled for a single overall treatment effect.

Conclusion: The D4C trial will generate evidence on whether a CDSS will further reduce the CVD burden among patients with diabetes beyond team-based care at community clinics. If proven effective, this implementation strategy could be scaled up within primary care settings in China and other LMICs to reduce CVD incidence and mortality among patients with diabetes.
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http://dx.doi.org/10.1016/j.ahj.2021.04.009DOI Listing
August 2021

"Hands" teaching method in colorectal anatomy: A model can be used in teaching and communication.

Asian J Surg 2021 Jul 28;44(7):982-983. Epub 2021 Apr 28.

The People's Hospital of Shinan District, Qingdao, Shandong Province, 266071, China.

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http://dx.doi.org/10.1016/j.asjsur.2021.04.018DOI Listing
July 2021

Raw data of laboratory toxicity and field control effect of different fungicides on .

Data Brief 2021 Jun 29;36:107000. Epub 2021 Mar 29.

College of Plant Protection, Hebei Agricultural University, Baoding, Hebei 071001, PR China.

Laboratory toxicity test and field control effect test are important experiments to evaluate the effect of pesticides on disease control. The safety of pesticide application depends on whether the pesticide residue on the fruit complies with the limits related to the good agricultural practices which were assessed by the legislative framework. In addition, many factors may affect the control effect of fungicides in the field experiment, among which one of the most important factors is the precipitation during the application process in the growing season. In this study, raw data of the laboratory toxicity of different fungicides to , disease severity after treatment with different agents, the pesticide residue on the fruit after preharvest application, and the precipitation in the growing season are provided. In addition, this study also introduced the method and calculation process of raw data processing. We hope that these raw data will serve as a reference for other researchers who are studying the prevention and control of Glomerella leaf spot (GLS).
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http://dx.doi.org/10.1016/j.dib.2021.107000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063743PMC
June 2021

Sodium Sensitivity, Sodium Resistance, and Incidence of Hypertension: A Longitudinal Follow-Up Study of Dietary Sodium Intervention.

Hypertension 2021 Jul 26;78(1):155-164. Epub 2021 Apr 26.

Department of Epidemiology and Key Laboratory of Cardiovascular Epidemiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences, Beijing, China (J.-F.H., X.L., J.-C.C., J.-X.L., J. Cao, D.-F.G.).

[Figure: see text].
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.120.16758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192427PMC
July 2021

Multi-ancestry genome-wide gene-sleep interactions identify novel loci for blood pressure.

Mol Psychiatry 2021 Apr 15. Epub 2021 Apr 15.

Department of Epidemiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

Long and short sleep duration are associated with elevated blood pressure (BP), possibly through effects on molecular pathways that influence neuroendocrine and vascular systems. To gain new insights into the genetic basis of sleep-related BP variation, we performed genome-wide gene by short or long sleep duration interaction analyses on four BP traits (systolic BP, diastolic BP, mean arterial pressure, and pulse pressure) across five ancestry groups in two stages using 2 degree of freedom (df) joint test followed by 1df test of interaction effects. Primary multi-ancestry analysis in 62,969 individuals in stage 1 identified three novel gene by sleep interactions that were replicated in an additional 59,296 individuals in stage 2 (stage 1 + 2 P < 5 × 10), including rs7955964 (FIGNL2/ANKRD33) that increases BP among long sleepers, and rs73493041 (SNORA26/C9orf170) and rs10406644 (KCTD15/LSM14A) that increase BP among short sleepers (P < 5 × 10). Secondary ancestry-specific analysis identified another novel gene by long sleep interaction at rs111887471 (TRPC3/KIAA1109) in individuals of African ancestry (P = 2 × 10). Combined stage 1 and 2 analyses additionally identified significant gene by long sleep interactions at 10 loci including MKLN1 and RGL3/ELAVL3 previously associated with BP, and significant gene by short sleep interactions at 10 loci including C2orf43 previously associated with BP (P < 10). 2df test also identified novel loci for BP after modeling sleep that has known functions in sleep-wake regulation, nervous and cardiometabolic systems. This study indicates that sleep and primary mechanisms regulating BP may interact to elevate BP level, suggesting novel insights into sleep-related BP regulation.
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http://dx.doi.org/10.1038/s41380-021-01087-0DOI Listing
April 2021

Jasmonates Alleviate the Harm of High-Temperature Stress During Anthesis to Stigma Vitality of Photothermosensitive Genetic Male Sterile Rice Lines.

Front Plant Sci 2021 29;12:634959. Epub 2021 Mar 29.

Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China.

Using photothermosensitive genic male sterile (PTSGMS) rice ( L.) lines to produce hybrids can obtain great heterosis. However, PTSGMS rice lines exhibit low stigma vitality when high-temperature (HT) stress happens during anthesis. Jasmonates (JAs) are novel phytohormones and play vital roles in mediating biotic and abiotic stresses. Little is known, however, if and how JAs could alleviate the harm of HT stress during anthesis to the stigma vitality of PTSGMS lines. This study investigated the question. Two PTSGMS lines and one restorer line of rice were pot-grown and subjected to normal temperature and HT stress during anthesis. The stigma exertion rate, sigma fresh weight, stigma area, contents of JAs, hydrogen peroxide (HO), and ascorbic acid (AsA), activity of catalase in stigmas, and the number of pollens germinated on the stigma of PTSGMS lines were determined. The results showed that a rice line with higher JAs content in the stigma under HT stress showed lower HO content, higher AsA content and catalase activity in stigmas, larger stigma area, heavier stigma fresh weight, more pollens germinated on the stigma, and higher fertilization and seed-setting and rates. Applying methyl JAs during anthesis to rice panicles decreased the accumulation of reactive oxygen species and enhanced stigma vitality, thereby increasing fertilization and seed-setting rates of the hybrids of PTSGMS rice lines under HT stress. The results demonstrate that JAs attenuate the injury of HT stress to the stigma vitality of PTSGMS rice lines through enhancing antioxidant ability.
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http://dx.doi.org/10.3389/fpls.2021.634959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039518PMC
March 2021

A novel metal organic gel with superior oxidase-like activity for efficient and sensitive chemiluminescence detection of uric acid.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Aug 1;257:119773. Epub 2021 Apr 1.

College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic address:

It is found that MIL-100(Fe) gels, as a kind of metal-organic gels (MOGs), constitutting of iron (Fe) and trimesic acid (HBTC), has been regarded as the efficient catalyst of luminol chemiluminescence (CL) system without the presence of extra oxidants in the present work. MIL-100(Fe) gels that have possessed mimicking oxidase-like activity can excellently enhanced luminol CL intensity by accelerating the generation of reactive oxygen species. Furthermore, with the addition of uric acid (UA), the CL signal has been dramatically inhibited under alkaline condition. Hence, the CL intensity inhibiting ratio (I/I) was proportional to the increasing concentration of UA in the rang from 10 nM to 4000 nM with the detection limit of 5.9 nM. This method has been successfully applied for analysis of UA with acceptable recoveries ranging from 97.0% to 107.9% in urine sample. These results indicates that this study open up a novel, sensitive and convenient method to detect UA in biological samples.
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http://dx.doi.org/10.1016/j.saa.2021.119773DOI Listing
August 2021

Chromosome Xq23 is associated with lower atherogenic lipid concentrations and favorable cardiometabolic indices.

Nat Commun 2021 04 12;12(1):2182. Epub 2021 Apr 12.

Division of Cardiology, George Washington University School of Medicine and Healthcare Sciences, Washington, DC, USA.

Autosomal genetic analyses of blood lipids have yielded key insights for coronary heart disease (CHD). However, X chromosome genetic variation is understudied for blood lipids in large sample sizes. We now analyze genetic and blood lipid data in a high-coverage whole X chromosome sequencing study of 65,322 multi-ancestry participants and perform replication among 456,893 European participants. Common alleles on chromosome Xq23 are strongly associated with reduced total cholesterol, LDL cholesterol, and triglycerides (min P = 8.5 × 10), with similar effects for males and females. Chromosome Xq23 lipid-lowering alleles are associated with reduced odds for CHD among 42,545 cases and 591,247 controls (P = 1.7 × 10), and reduced odds for diabetes mellitus type 2 among 54,095 cases and 573,885 controls (P = 1.4 × 10). Although we observe an association with increased BMI, waist-to-hip ratio adjusted for BMI is reduced, bioimpedance analyses indicate increased gluteofemoral fat, and abdominal MRI analyses indicate reduced visceral adiposity. Co-localization analyses strongly correlate increased CHRDL1 gene expression, particularly in adipose tissue, with reduced concentrations of blood lipids.
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http://dx.doi.org/10.1038/s41467-021-22339-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042019PMC
April 2021

CAP Analysis of the Distribution of the Introduced Bemisia tabaci (Hemiptera: Aleyrodidae) Species Complex in Xinjiang, China and the Southerly Expansion of the Mediterranean Species.

J Insect Sci 2021 Mar;21(2)

Institute of Microbial Application, Xinjiang Academy of Agricultural Sciences, Xinjiang, China.

Bemisia tabaci (Gennadius) cryptic complex has invaded Xinjiang, China, since 1998. The distribution of Mediterranean (MED) and Middle East-Asia Minor 1 (MEAM1) B. tabaci substrains has been gradually identified due to the development of molecular technology. In this study, the distribution of MED and MEAM1 in Xinjiang was determined by cleaved amplified polymorphic sequence (CAPs). Results showed that MED dominated in northern Xinjiang (84%), whereas MEAM1 was dominant in southern Xinjiang (72%). Five pairs of simple sequence repeat (SSR) primers were used to analyze the genetic diversity of B. tabaci among 36 geographic populations. The genetic diversity of MED and MEAM1was low and varied little among populations in Xinjiang (0.09 ± 0.14 and 0.09 ± 0.13, respectively). Based on ∆K statistic, 13 populations of MEAM1 could be classified into two subgroups at K = 2, whereas the 23 populations of MED could be classified into four subgroups at K = 4. However, Mantel t-test demonstrated no correlation between geographical and genetic distances among B. tabaci complex (R = 0.42, P = 1.00). Neighbor-joining and principal coordinate analysis showed that geographical isolation and interspecific differences were the main causes of the genetic variation. Gene flow predicted that MEAM1 was most likely introduced from Urumqi to the southern Xinjiang. Meanwhile, a large proportion of MED in Kashi region came from Changji and Yining. To block ongoing dispersal, strict detection and flower quarantine regulations need to be enforced.
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http://dx.doi.org/10.1093/jisesa/ieaa151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040787PMC
March 2021