Publications by authors named "He Gang"

298 Publications

Dye-loaded mesoporous polydopamine nanoparticles for multimodal tumor theranostics with enhanced immunogenic cell death.

J Nanobiotechnology 2021 Nov 17;19(1):365. Epub 2021 Nov 17.

Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, People's Republic of China.

Background: Tumor phototherapy especially photodynamic therapy (PDT) or photothermal therapy (PTT), has been considered as an attractive strategy to elicit significant immunogenic cell death (ICD) at an optimal tumor retention of PDT/PTT agents. Heptamethine cyanine dye (IR-780), a promising PDT/PTT agent, which can be used for near-infrared (NIR) fluorescence/photoacoustic (PA) imaging guided tumor phototherapy, however, the strong hydrophobicity, short circulation time, and potential toxicity in vivo hinder its biomedical applications. To address this challenge, we developed mesoporous polydopamine nanoparticles (MPDA) with excellent biocompatibility, PTT efficacy, and PA imaging ability, facilitating an efficient loading and protection of hydrophobic IR-780.

Results: The IR-780 loaded MPDA ([email protected]) exhibited high loading capacity of IR-780 (49.7 wt%), good physiological solubility and stability, and reduced toxicity. In vivo NIR fluorescence and PA imaging revealed high tumor accumulation of [email protected] Furthermore, the combined PDT/PTT of [email protected] could induce ICD, triggered immunotherapeutic response to breast tumor by the activation of cytotoxic T cells, resulting in significant suppression of tumor growth in vivo.

Conclusion: This study demonstrated that the as-developed compact and biocompatible platform could induce combined PDT/PTT and accelerate immune activation via excellent tumor accumulation ability, offering multimodal tumor theranostics with negligible systemic toxicity.
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http://dx.doi.org/10.1186/s12951-021-01109-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596951PMC
November 2021

Effects of aging on the histology and biochemistry of rat tendon healing.

BMC Musculoskelet Disord 2021 Nov 15;22(1):949. Epub 2021 Nov 15.

Department of Sports Medicine Center, Southwest Hospital, Army Medical University, Chongqing, 400038, China.

Introduction: Tendon diseases and injuries are a serious problem for the aged population, often leading to pain, disability and a significant decline in quality of life. The purpose of this study was to determine the influence of aging on biochemistry and histology during tendon healing and to provide a new strategy for improving tendon healing.

Method: A total of 24 Sprague-Dawley rats were equally divided into a young and an aged group. A rat patellar tendon defect model was used in this study. Tendon samples were collected at weeks 2 and 4, and hematoxylin-eosin, alcian blue and immunofluorescence staining were performed for histological analysis. Meanwhile, reverse transcription-polymerase chain reaction (RT-PCR) and western blot were performed to evaluate the biochemical changes.

Results: The histological scores in aged rats were significantly lower than those in young rats. At the protein level, collagen synthesis-related markers Col-3, Matrix metalloproteinase-1 and Metallopeptidase Inhibitor 1(TIMP-1) were decreased at week 4 in aged rats compared with those of young rats. Though there was a decrease in the expression of the chondrogenic marker aggrecan at the protein level in aged tendon, the Micro-CT results from weeks 4 samples showed no significant difference(p>0.05) on the ectopic ossification between groups. Moreover, we found more adipocytes accumulated in the aged tendon defect with the Oil Red O staining and at the gene and protein levels the markers related to adipogenic differentiation.

Conclusions: Our findings indicate that tendon healing is impaired in aged rats and is characterized by a significantly lower histological score, decreased collagen synthesis and more adipocyte accumulation in patellar tendon after repair.
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http://dx.doi.org/10.1186/s12891-021-04838-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594129PMC
November 2021

MiR-6924-5p-rich exosomes derived from genetically modified Scleraxis-overexpressing PDGFRα(+) BMMSCs as novel nanotherapeutics for treating osteolysis during tendon-bone healing and improving healing strength.

Biomaterials 2021 Nov 5;279:121242. Epub 2021 Nov 5.

Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, Burn and Combined Injury, First Affiliated Hospital of Third Military Medical University (Army Medical University), Chongqing, 400000, China. Electronic address:

Osteolysis at the tendon-bone interface can impair pullout strength during tendon-bone healing and lead to surgery failure, but the effects of clinical treatments are not satisfactory. Mesenchymal stem cell (MSC)-derived exosomes have been used as potent and feasible natural nanocarriers for drug delivery and have been proven to enhance tendon-bone healing strength, indicating that MSC-derived exosomes could be a promising therapeutic strategy. In this study, we explored Scleraxis (Scx) dynamically expressed in PDGFRα(+) bone marrow-derived mesenchymal stem cells (BMMSCs) during natural tendon-bone healing. Then, we investigated the role of PDGFRα(+) BMMSCs in tendon-bone healing after Scx overexpression as well as the underlying mechanisms. Our data demonstrated that Scx-overexpressing PDGFRα(+) BMMSCs (BMMSC) could efficiently inhibit peritunnel osteolysis and enhance tendon-bone healing strength by preventing osteoclastogenesis in an exosomes-dependent manner. Exosomal RNA-seq revealed that the abundance of a novel miRNA, miR-6924-5p, was highest among miRNAs. miR-6924-5p could directly inhibit osteoclast formation by binding to the 3'-untranslated regions (3'UTRs) of OCSTAMP and CXCL12. Inhibition of miR-6924-5p expression reversed the prevention of osteoclastogenic differentiation by BMMSC derived exosomes (BMMSC-exos). Local injection of BMMSC-exos or miR-6924-5p dramatically reduced osteoclast formation and improved tendon-bone healing strength. Furthermore, delivery of miR-6924-5p efficiently inhibited the osteoclastogenesis of human monocytes. In brief, our study demonstrates that BMMSC-exos or miR-6924-5p could serve as a potential therapy for the treatment of osteolysis during tendon-bone healing and improve the outcome.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121242DOI Listing
November 2021

Adipogenic differentiation was inhibited by downregulation of PPARγ signaling pathway in aging tendon stem/progenitor cells.

J Orthop Surg Res 2021 Oct 18;16(1):614. Epub 2021 Oct 18.

Department of Sports Medicine Center, Southwest Hospital, Army Medical University, Chongqing, 400038, China.

Background: Tendon stem/progenitor cells (TSPCs) play a vital role in tendon repair and regeneration. Previously we found more adipocytes accumulated in the patellar tendon injury sites in aging rats compared with the young ones, of which the mechanism is still unknown. Here, we want to identify whether erroneous differentiation of TSPCs by aging accounts for the adipocyte accumulation.

Methods: TSPCs from young and aging rats were isolated and propagated. Both young and aging TSPCs were induced to differentiate into adipocytes, and Oil red O staining, quantitative real-time polymerase chain reaction (qRT-PCR), western-blot and immunofluorescent staining were used to evaluate the capability of TSPCs. RNA sequencing was utilized to screen out different genes and signaling pathways related to adipogenesis between young and aging TSPCs.

Results: The Oil red O staining showed there were more adipocytes formed in young TSPCs. Besides, adipogenic markers perilipin, peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding proteins alpha (C/EBPα) and Fatty acid-binding protein 4 (FABP4) were elevated both at gene and protein level. PPARγ signaling pathway was selected as our target via RNA sequencing. After adding the signaling activators, Rosiglitazone maleate (RM), inhibited adipogenesis of aging TSCs was reversed.

Conclusions: In conclusion, aging inhibited adipogenesis of TSPCs by down-regulating PPARγ signaling. It is not likely that the adipocyte accumulation in aging tendon during repair was due to the aging of TSPCs. This may provide new targets for curing aging tendon injuries or tendinopathies.
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http://dx.doi.org/10.1186/s13018-021-02720-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522149PMC
October 2021

Altered Lipid Profile Is a Risk Factor for the Poor Progression of COVID-19: From Two Retrospective Cohorts.

Front Cell Infect Microbiol 2021 30;11:712530. Epub 2021 Sep 30.

Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Background: The coronavirus disease 2019 (COVID-19) pandemic has spread worldwide. However, the impact of baseline lipid profile on clinical endpoints in COVID-19 and the potential effect of COVID-19 on lipid profile remain unclear.

Methods: In this retrospective cohort study, we consecutively enrolled 430 adult COVID-19 patients from two Chinese hospitals (one each in Chengdu and Wuhan). The lipid profile before admission and during the disease course and the clinical endpoint including in-hospital death or oropharyngeal swab test positive again (OSTPA) after discharge were collected. We used Kaplan-Meier and Cox regression to explore the lipid risk factors before admission associated with endpoints. Then, we assessed the lipid level change along with the disease course to determine the relationship between pathology alteration and the lipid change.

Results: In the Chengdu cohort, multivariable Cox regression showed that low-density lipoprotein cholesterol (LDL-C) dyslipidemia before admission was associated with OSTPA after discharge for COVID-19 patients (RR: 2.51, 95% CI: 1.19, 5.29, p = 0.006). In the Wuhan cohort, the patients with triglyceride (TG) dyslipidemia had an increased risk of in-hospital death (RR: 1.92, 95% CI: 1.08, 3.60, p = 0.016). In addition, in both cohorts, the lipid levels gradually decreased in the in-hospital death or OSTPA subgroups since admission. On admission, we also noticed the relationship between the biomarkers of inflammation and the organ function measures and this lipid level in both cohorts. For example, after adjusting for age, sex, comorbidities, smoking, and drinking status, the C-reactive protein level was negatively associated with the TC lipid level [β (SE) = -0.646 (0.219), = 0.005]. However, an increased level of alanine aminotransferase, which indicates impaired hepatic function, was positively associated with total cholesterol (TC) lipid levels in the Chengdu cohort [β (SE) = 0.633 (0.229), = 0.007].

Conclusions: The baseline dyslipidemia should be considered as a risk factor for poor prognosis of COVID-19. However, lipid levels may be altered during the COVID-19 course, since lipidology may be distinctly affected by both inflammation and organic damage for SARS-CoV-2.
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http://dx.doi.org/10.3389/fcimb.2021.712530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515140PMC
October 2021

Study on ecological security evaluation of Anhui Province based on normal cloud model.

Authors:
Gang He Jun Ruan

Environ Sci Pollut Res Int 2021 Oct 14. Epub 2021 Oct 14.

School of Economy and Management, Anhui University of Science and Technology, Huainan, China.

As the main driving force for the economic development of East China, the economic development of Anhui has been in good state in recent years. In 2019, the GDP of Anhui Province has reached 3711.4 billion yuan with an increase of 7.5% over the previous year. With the rapid economic development, many environmental problems such as resource depletion, vegetation degradation, and air quality decline have gradually increased. The ecological environment is an important foundation for social and economic development. To examine the status of regional ecological security, this study builds a comprehensive evaluation index system based on the "pressure-state-response" (PSR) framework and uses the entropy weight method to determine the index weight. Then the normal cloud model is introduced to dynamically calculate the ecological security status of Anhui Province from 2010 to 2019. The results show that (1) the ecological security status of Anhui has improved significantly and reached a safe status in 2019. (2) The ecological security status of 16 prefecture-level cities has also been improved, and the ecological security status of these cities is at level III and above except Huainan. There are significant differences in internal development, and the ecological security status of the southern region is ahead of the northern and central region. (3) From the perspective of the criterion level, the pressure and response subsystems develop faster, and the state subsystem develops more slowly. Therefore, it is necessary to pay more attention to the impact of the state subsystem on the overall development of ecological security in the future.
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http://dx.doi.org/10.1007/s11356-021-16896-7DOI Listing
October 2021

Construction of Peptide Macrocycles via Palladium-Catalyzed Multiple S-Arylation: An Effective Strategy to Expand the Structural Diversity of Cross-Linkers.

Org Lett 2021 10 28;23(20):8001-8006. Epub 2021 Sep 28.

State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

A simple and versatile method for macrocyclizing unprotected native peptides with a wide range of easily accessible diiodo and triiodoarene reagents via the palladium-catalyzed multiple S-arylation of cysteine residues is developed. Iodoarenes with different arene and heteroarene cores can be incorporated into peptide macrocycles of varied ring sizes and amino acid compositions with high efficiency and selectivity under mild conditions.
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http://dx.doi.org/10.1021/acs.orglett.1c03003DOI Listing
October 2021

Inorganic Nanomaterials with Intrinsic Singlet Oxygen Generation for Photodynamic Therapy.

Adv Sci (Weinh) 2021 11 24;8(21):e2102587. Epub 2021 Sep 24.

State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P.R. China.

Inorganic nanomaterials with intrinsic singlet oxygen ( O ) generation capacity, are emerged yet dynamically developing materials as nano-photosensitizers (NPSs) for photodynamic therapy (PDT). Compared to previously reported nanomaterials that have been used as either carriers to load organic PSs or energy donors to excite the attached organic PSs through a Foster resonance energy transfer process, these NPSs possess intrinsic O generation capacity with extremely high O quantum yield (e.g., 1.56, 1.3, 1.26, and 1.09) than any classical organic PS reported to date, and thus are facilitating to make a revolution in PDT. In this review, the recent advances in the development of various inorganic nanomaterials as NPSs, including metal-based (gold, silver, and tungsten), metal oxide-based (titanium dioxide, tungsten oxide, and bismuth oxyhalide), metal sulfide-based (copper and molybdenum sulfide), carbon-based (graphene, fullerene, and graphitic carbon nitride), phosphorus-based, and others (hybrids and MXenes-based NPSs) are summarized, with an emphasis on the design principle and O generation mechanism, and the photodynamic therapeutic performance against different types of cancers. Finally, the current challenges and an outlook of future research are also discussed. This review may provide a comprehensive account capable of explaining recent progress as well as future research of this emerging paradigm.
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http://dx.doi.org/10.1002/advs.202102587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564446PMC
November 2021

Plausible Role of Asthma Biological Modifiers in the Treatment of Eosinophilic Esophagitis.

Cureus 2021 Jul 18;13(7):e16460. Epub 2021 Jul 18.

Allergy and Immunology, Donald and Barbara Zucker School of Medicine at Hofstra / Northwell, Hempstead, USA.

Eosinophilic esophagitis (EoE) is a T-helper type-2 (Th2/T2) cell-mediated disease characterized by 15 or more eosinophils per high-powered esophageal biopsy microscopy field (eos/hpf), excluding other causes. EoE is often clinically characterized by symptoms such as dysphagia, nausea, food impaction, and chest pain that do not respond to antacids. Two-thirds of patients are unresponsive to proton pump inhibitors (PPIs). Steroids may be effective but pose long-term health risks and can lose efficacy in patients with serum eosinophilia greater than 1,500 cells/µL. Because EoE is not IgE-mediated, allergy skin testing for food may benefit a subset of patients. These therapies have shortcomings, which necessitate further investigation. Herein, we report a patient successfully treated with benralizumab (anti-IL-5Rα), demonstrating a potential solution to the lack of effective treatments for EoE.
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http://dx.doi.org/10.7759/cureus.16460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370573PMC
July 2021

The Impact of Glycolipid Metabolic Disorders on Severity Stage-Specific Variation of Cardiac Autonomic Function in Obstructive Sleep Apnea: A Data-Driven Clinical Study.

Nat Sci Sleep 2021 28;13:1347-1362. Epub 2021 Jul 28.

Department of Otolaryngology Head and Neck Surgery & Center of Sleep Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China.

Background: Cardiac autonomic dysfunction (CAD) is a common pathology in cardiovascular diseases; however, the role of glycolipid metabolic disorders in CAD development in obstructive sleep apnea (OSA) remains poorly understood.

Methods: In total, 4152 patients with suspected OSA were recruited in our sleep center. Metabolic characteristics including anthropometric and glycolipid data were collected. Heart rate variability (HRV) was measured to assess the risk of CAD; its dose-response relationship with OSA severity was evaluated via restricted cubic spline (RCS) analysis. A segmented multivariate linear regression (SMLR) model was used to evaluate the roles of metabolic variables in different stages of OSA.

Results: The RCS showed that CAD risk increased in a nonlinear relationship pattern with OSA severity, from slow fluctuation at earlier stages to rapid change in later stages. After integrating the clinical definition and RCS selected knots, we obtained the new four OSA severity stages. SMLR model showed that the overall value of glycolipid variables for prediction of HRV abnormalities was greater than the value of OSA variables at earlier stages, while OSA variables were more effective predictors in more severe stages. The discordance in respective relationship of HRV with metabolic and OSA variables sheds the light how metabolic disorders promoted the development of CAD in OSA, the later further in turn deteriorates cardiac function.

Conclusion: These results are indicative of stage-specific involvement of glycolipid metabolic factors underlying CAD nonlinear changes in patients with OSA. Early control glycolipid disorders may help the control of CAD development in patients with OSA.
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http://dx.doi.org/10.2147/NSS.S317201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327800PMC
July 2021

Anti-Sandwich Structured Photo-Electronic Wound Dressing for Highly Efficient Bacterial Infection Therapy.

Small 2021 08 11;17(33):e2101858. Epub 2021 Jul 11.

Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, 710049, China.

Photo-electronic devices based on reactive oxygen species (ROS) generation suffer a crucial limitation in wound treatment due to their sandwich structure, which prevents the contact of ROS with wound tissue. In this work, the first anti-sandwich structured visible-light/electricity dual-responsive wound dressing is constructed for treatment of methicillin-resistant Staphylococcus aureus (MRSA), based on selenoviologen-appendant polythiophene (SeV -PT)-containing polyacrylamide hydrogels. The new wound dressing is named an anti-sandwich structured photo-electronic wound dressing (PEWD). The unique structure of PEWD enables its use in synergistic electrodynamic and photodynamic therapy (EDT and PDT), providing rapid, on-demand, and sustained generation of ROS in situ via short-time light irradiation and/or wireless-controlled electrification. The PEWD possesses good flexibility, excellent biocompatibility, and fast response, as well as sustained ROS generation in a physiological environment. Animal experiments demonstrate effective ROS generation in 6 s under irradiation and electrification, inhibiting infection at an early stage, and substantially shortening the healing time of bacterial infection (to within 7 days). This proof-of-concept research holds great promise in developing new flexible PEWD, and novel strategies to improve wound treatment.
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http://dx.doi.org/10.1002/smll.202101858DOI Listing
August 2021

Stereoselective Synthesis of C-Vinyl Glycosides via Palladium-Catalyzed C-H Glycosylation of Alkenes.

Angew Chem Int Ed Engl 2021 09 6;60(36):19620-19625. Epub 2021 Aug 6.

State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071, China.

C-vinyl glycosides are an important class of carbohydrates and pose a unique synthetic challenge. A new strategy has been developed for stereoselective synthesis of C-vinyl glycosides via Pd-catalyzed directed C-H glycosylation of alkenes with glycosyl chloride donors using an easily removable bidentate auxiliary. Both the γ C-H bond of allylamines and the δ C-H bond of homoallyl amine substrates can be glycosylated in high efficiency and with excellent regio- and stereoselectivity. The resulting C-vinyl glycosides can be further converted to a variety of C-alkyl glycosides with high stereospecificity. These reactions offer a broadly applicable method to streamline the synthesis of complex C-vinyl glycosides from easily accessible starting materials.
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http://dx.doi.org/10.1002/anie.202104430DOI Listing
September 2021

Use of historical and contemporary distribution of mammals in China to inform conservation.

Conserv Biol 2021 Dec 5;35(6):1787-1796. Epub 2021 Jul 5.

Shaanxi Key Laboratory for Animal Conservation, College of Life Sciences, Northwest University, Xi'an, China.

A systematic understanding of dynamic animal extinction trajectories for different regions in a nation like China is critically important to developing practical conservation strategies. We explored historical and contemporary changes in terrestrial mammalian diversity to determine how diversity in each of the 5 regions in China has changed over time and to examine the conservation potential of these regions. We used records from databases on Pleistocene mammalian fossils and historical distribution records (1175-2020) for Primates (as a case study) to reconstruct evolutionary and historical distribution trajectories of the 11 orders of terrestrial mammals and to predict their prospective survival based on the national conservation strategy applied. The results indicated that since the Pleistocene, 4-5 mammalian orders have been lost in the northeast, 3 in central China, 2 along the coast, and 1 in the northwest. In the southwest, all 11 orders were maintained. Contemporarily, the coast and southwest had the highest and second-highest species densities. The southwest region and southeastern sections of the northwest region were the most historically and contemporarily diverse areas, which suggests that they should be the first priority for protected area (PA) designation. The central and coastal areas should be secondarily prioritized. In these 2 regions, conservation should focus on human coexistence with nature. Less attention should be paid to the PA in the northeast and western northwest because in these areas ecosystems are depauperate and the climate is harsh. Conservation in these areas should focus principally on avoiding further human encroachment on natural areas. Article impact statement: Historical and contemporary patterns of extinction can be a basis for mammalian conservation strategies.
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http://dx.doi.org/10.1111/cobi.13795DOI Listing
December 2021

Enabling a Rapid and Just Transition away from Coal in China.

One Earth 2020 Aug;3(2):187-194

Natural Resources Defense Council China Program, Beijing 100026, China.

As the world's largest coal producer and consumer, China's transition from coal to cleaner energy sources is critical for achieving global decarbonization. Increasing regulations on air pollution and carbon emissions and decreasing costs of renewables drive China's transition away from coal; however, this transition also has implications for employment and social justice. Here, we assess China's current coal-transition policies, their barriers, and the potential for an accelerated transition, as well as the associated environmental, human health, and employment and social justice issues that may arise from the transition. We estimate that the most aggressive coal-transition pathway could reduce annual premature death related to coal combustion by 224,000 and reduce annual water consumption by 4.3 billion m in 2050 compared with business-as-usual. We highlight knowledge gaps and conclude with policy recommendations for an integrated approach to facilitate a rapid and just transition away from coal in China.
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http://dx.doi.org/10.1016/j.oneear.2020.07.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442150PMC
August 2020

Streamlined construction of peptide macrocycles palladium-catalyzed intramolecular -arylation in solution and on DNA.

Chem Sci 2021 Mar 11;12(16):5804-5810. Epub 2021 Mar 11.

State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University Tianjin 300071 China

A highly efficient and versatile method for construction of peptide macrocycles palladium-catalyzed intramolecular -arylation of alkyl and aryl thiols with aryl iodides under mild conditions is developed. The method exhibits a broad substrate scope for thiols, aryl iodides and amino acid units. Peptide macrocycles of a wide range of size and composition can be readily assembled in high yield from various easily accessible building blocks. This method has been successfully employed to prepare an 8-million-membered tetrameric cyclic peptide DNA-encoded library (DEL). Preliminary screening of the DEL library against protein p300 identified compounds with single digit micromolar inhibition activity.
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http://dx.doi.org/10.1039/d1sc00789kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179660PMC
March 2021

Injectable Self-Healing Adhesive pH-Responsive Hydrogels Accelerate Gastric Hemostasis and Wound Healing.

Nanomicro Lett 2021 Feb 27;13(1):80. Epub 2021 Feb 27.

Department of Gastroenterology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China.

Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) are well-established therapeutics for gastrointestinal neoplasias, but complications after EMR/ESD, including bleeding and perforation, result in additional treatment morbidity and even threaten the lives of patients. Thus, designing biomaterials to treat gastric bleeding and wound healing after endoscopic treatment is highly desired and remains a challenge. Herein, a series of injectable pH-responsive self-healing adhesive hydrogels based on acryloyl-6-aminocaproic acid (AA) and AA-g-N-hydroxysuccinimide (AA-NHS) were developed, and their great potential as endoscopic sprayable bioadhesive materials to efficiently stop hemorrhage and promote the wound healing process was further demonstrated in a swine gastric hemorrhage/wound model. The hydrogels showed a suitable gelation time, an autonomous and efficient self-healing capacity, hemostatic properties, and good biocompatibility. With the introduction of AA-NHS as a micro-cross-linker, the hydrogels exhibited enhanced adhesive strength. A swine gastric hemorrhage in vivo model demonstrated that the hydrogels showed good hemostatic performance by stopping acute arterial bleeding and preventing delayed bleeding. A gastric wound model indicated that the hydrogels showed excellent treatment effects with significantly enhanced wound healing with type I collagen deposition, α-SMA expression, and blood vessel formation. These injectable self-healing adhesive hydrogels exhibited great potential to treat gastric wounds after endoscopic treatment.
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http://dx.doi.org/10.1007/s40820-020-00585-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187506PMC
February 2021

Individual mortality risk predictive system of patients with acute-on-chronic liver failure based on a random survival forest model.

Chin Med J (Engl) 2021 Jun 16;134(14):1701-1708. Epub 2021 Jun 16.

Department of Infectious Diseases, The First People's Hospital of Foshan, Foshan, Guangdong 528000, China.

Background: The basis of individualized treatment should be individualized mortality risk predictive information. The present study aimed to develop an online individual mortality risk predictive tool for acute-on-chronic liver failure (ACLF) patients based on a random survival forest (RSF) algorithm.

Methods: The current study retrospectively enrolled ACLF patients from the Department of Infectious Diseases of The First People's Hospital of Foshan, Shunde Hospital of Southern Medical University, and Jiangmen Central Hospital. Two hundred seventy-six consecutive ACLF patients were included in the present study as a model cohort (n = 276). Then the current study constructed a validation cohort by drawing patients from the model dataset based on the resampling method (n = 276). The RSF algorithm was used to develop an individual prognostic model for ACLF patients. The Brier score was used to evaluate the diagnostic accuracy of prognostic models. The weighted mean rank estimation method was used to compare the differences between the areas under the time-dependent ROC curves (AUROCs) of prognostic models.

Results: Multivariate Cox regression identified hepatic encephalopathy (HE), age, serum sodium level, acute kidney injury (AKI), red cell distribution width (RDW), and international normalization index (INR) as independent risk factors for ACLF patients. A simplified RSF model was developed based on these previous risk factors. The AUROCs for predicting 3-, 6-, and 12-month mortality were 0.916, 0.916, and 0.905 for the RSF model and 0.872, 0.866, and 0.848 for the Cox model in the model cohort, respectively. The Brier scores were 0.119, 0.119, and 0.128 for the RSF model and 0.138, 0.146, and 0.156 for the Cox model, respectively. The nonparametric comparison suggested that the RSF model was superior to the Cox model for predicting the prognosis of ACLF patients.

Conclusions: The current study developed a novel online individual mortality risk predictive tool that could predict individual mortality risk predictive curves for individual patients. Additionally, the current online individual mortality risk predictive tool could further provide predicted mortality percentages and 95% confidence intervals at user-defined time points.
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http://dx.doi.org/10.1097/CM9.0000000000001539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318661PMC
June 2021

Alloy Engineering of a Polar (Si,Ge)NO System for Controllable Second Harmonic Performance.

Inorg Chem 2021 May 27;60(10):7381-7388. Epub 2021 Apr 27.

Beijing Computational Science Research Center, Beijing 100193, China.

Although silicon oxynitrides are important semiconductors for many practical applications, their second-order nonlinear optical (NLO) properties have never been systemically explored. Using the first-principles calculations, in this article, we discover that the sinoite (e.g., silicon oxynitride SiNO) can simultaneously exhibit wide optical band gap, strong second-harmonic generation (SHG) effect, and large birefringence, which are further confirmed by our preliminary experimental measurement. Importantly, we propose that alloying engineering can be further applied to control the balanced NLO properties in the SiNO system. Based on first-principles cluster expansion theory, we demonstrate that alloying Ge into SiNO can form low formation-energy SiGeNO alloys, which can in turn achieve controllable phase-matching harmonic output with high SHG efficiency at different energy ranges. Therefore, alloy engineering could provide a unique approach to effectively control the NLO performance of SiGeNO, making this polar alloy system hold potential applications in tunable laser conversion and controllable optical devices.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00590DOI Listing
May 2021

Photoredox-Mediated Mono- and Difluorination of Remote Unactivated Methylene C(sp)-H Bonds of -Alkyl Sulfonamides.

Org Lett 2021 05 21;23(9):3631-3635. Epub 2021 Apr 21.

State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

A photoredox-mediated δ-C(sp)-H fluorination of sulfonyl-protected primary alkylamines with Selectfluor is developed. The reaction can proceed in excellent monofluorination selectivity for amine substrates without α substituent. For α-substituted substrates, a slightly modified reaction conditions with two rounds of operation gives the δ,δ-difluorination products in good yield. Mechanistic studies suggest SET oxidation of sulfonamide group directly generates the key sulfonamide N radical intermediate, which triggers a 1,5-HAT process to form the δ alkyl radical.
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http://dx.doi.org/10.1021/acs.orglett.1c01020DOI Listing
May 2021

Microplastic contamination is ubiquitous in riparian soils and strongly related to elevation, precipitation and population density.

J Hazard Mater 2021 06 19;411:125178. Epub 2021 Jan 19.

CAS Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China; Hubei Key Laboratory of Wetland Evolution and Ecological Restoration, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China. Electronic address:

Although environmental research has recently begun to focus on the ubiquity of microplastics in terrestrial systems, there is still lack of comprehensive data which describe microplastics levels in soils and the factors influencing the distribution of this contaminant. Here, we show that microplastics contamination (3877 ± 2356 p kg) is omnipresent in numerous soil samples collected along the Yangtze River. Subsoils (4005 ± 2472 p kg) showed higher levels of microplastics than topsoils (3748 ± 2301 p kg), while polyamide (32%) was the most commonly found polymer in the samples. Small microplastics particles (< 200 µm) accounted for approximately 70% of the microplastics detected in subsoils. In terms of shape, microfragments were the most common type of microplastic particle, accounting for 34% of total microplastics, followed by microfibers (30%). Furthermore, microplastics contamination was found to be positively correlated with both the population of the study area and precipitation, yet negatively correlated with the elevation of the sampling site. Our study represents the first large-scale study of microplastic contamination in riparian soils along the Yangtze River, and provides important data regarding the ecotoxicology and ecosystem effects of microplastics in terrestrial environments.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125178DOI Listing
June 2021

β-Lactam Synthesis via Copper-Catalyzed Directed Aminoalkylation of Unactivated Alkenes with Cyclobutanone -Benzoyloximes.

Org Lett 2021 05 14;23(9):3620-3625. Epub 2021 Apr 14.

State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

A new protocol for amide-directed Cu-catalyzed aminoalkylation of unactivated alkenes using cyclobutanone oxime esters as alkyl radical donors is developed. Both primary and secondary alkyl groups can be selectively installed at the C4 position of terminal or -internal 3-alkenamides in moderate to good yield. This reaction offers a useful method for the diastereoselective synthesis of β-lactams bearing 4-cyanoalkyl β-substituents. The use of a weakly coordinating counteranion as the Cu catalyst is critical for the formation of β-lactam products.
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http://dx.doi.org/10.1021/acs.orglett.1c01007DOI Listing
May 2021

Corrigendum to "Bionic Silk Fibroin Film Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells by Activating Focal Adhesion Kinase".

Stem Cells Int 2021 2;2021:6317062. Epub 2021 Mar 2.

Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China.

[This corrects the article DOI: 10.1155/2020/8857380.].
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http://dx.doi.org/10.1155/2021/6317062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946452PMC
March 2021

Nitrene-mediated intermolecular N-N coupling for efficient synthesis of hydrazides.

Nat Chem 2021 04 22;13(4):378-385. Epub 2021 Mar 22.

State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, China.

N-N linkages are found in many natural compounds and endow fascinating structural and functional properties. In comparison to the myriad methods for the construction of C-N bonds, chemistry for N-N coupling, especially in an intermolecular fashion, remains underdeveloped. Here, we report a nitrene-mediated intermolecular N-N coupling of dioxazolones and arylamines under iridium or iron catalysis. These reactions offer a simple and efficient method for the synthesis of various hydrazides from readily available carboxylic acid and amine precursors. Although the Ir-catalysed conditions usually give higher N-N coupling yield than the Fe-catalysed conditions, the reactions of sterically more demanding dioxazolones derived from α-substituted carboxylic acids work much better under the Fe-catalysed conditions. Mechanistic studies revealed that the nitrogen atom of Ir acyl nitrene intermediates has strong electrophilicity and can undergo nucleophilic attack with arylamines with the assistance of Cl···HN hydrogen bonding to form the N-N bond with high efficiency and chemoselectivity.
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http://dx.doi.org/10.1038/s41557-021-00650-0DOI Listing
April 2021

Tetraphenylethylene-Based Multicomponent Emissive Metallacages as Solid-State Fluorescent Materials.

Angew Chem Int Ed Engl 2021 May 19;60(22):12293-12297. Epub 2021 Apr 19.

State Key Laboratory for Mechanical Behavior of Materials, Shaanxi International Research Center for Soft Matter, School of Materials Science and Engineering, Xi'an Jiaotong University, Xi'an, 710049, P. R. China.

The construction of solid-state fluorescent materials with high quantum yield and good processability is of vital importance in the preparation of organic light-emitting devices. Herein, a series of tetraphenylethylene (TPE)-based multicomponent emissive metallacages are prepared by the coordination-driven self-assembly of tetra-(4-pyridylphenyl)ethylene, cis-Pt(PEt ) (OTf) and tetracarboxylic ligands. These metallacages exhibit good emission both in solution and in the solid state because the coordination bonds and aggregation restrict the molecular motions of TPE synergistically, which suppresses the non-radiative decay of these metallacages. Impressively, one of the metallacages achieves very high fluorescence quantum yield (Φ =88.46 %) in the solid state, which is further used as the coatings of a blue LED bulb to achieve white-light emission. The study not only provides a general method to the preparation of TPE-based metallacages but also explores their applications as solid-state fluorescent materials, which will promote the future design and applications of metallacages as useful emissive devices.
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http://dx.doi.org/10.1002/anie.202100463DOI Listing
May 2021

Adaptation and evaluation of the neighborhood environment walkability scale for youth for Chinese children (NEWS-CC).

BMC Public Health 2021 03 11;21(1):480. Epub 2021 Mar 11.

Department of Sports Science and Physical Education, The Chinese University of Hong Kong, Shatin, Hong Kong, China.

Background: The physical activity-environment relationship has been infrequently investigated in Chinese children. Reliable and valid environmental measures specific to the age group and the local context are crucial for better understanding this relationship. The purposes of this study were to adapt the Neighborhood Environment Walkability Scale for youth (NEWS-Y) for Chinese children (termed NEWS-CC), and to examine the reliability and factorial validity of the NEWS-CC.

Methods: The development of the NEWS-CC involved the translation of the NEWS-Y to Chinese and the addition of nine new items capturing Hong Kong specific environmental attributes which were generated in our previous study. A total of 953 Hong Kong children aged 9-14 years volunteered to complete the NEWS-CC twice with 7-14 days apart. Confirmatory factor analysis (CFA) was performed to examine the factorial validity of the NEWS-CC. Test-retest reliability of subscales and individual items in the NEWS-CC was examined by intraclass correlation coefficients (ICCs).

Results: The CFA identified a 7-factor measurement model for the NEWS-CC which fitted the data well, with an additional "pollution" factor not included in the original NEWS-Y. The final NEWS-CC consisted of 67 items in 10 subscales. The test-retest reliability of subscales (range of ICC = 0.47-0.86) and individual items (range of ICC = 0.41-0.79) in the final NEWS-CC was moderate to good.

Conclusion: The results of this study support the psychometric properties of the NEWS-CC. The NEWS-CC can be used to assess physical activity-related neighborhood environment among children in Hong Kong, as well as cities that share similar urban forms with Hong Kong.
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http://dx.doi.org/10.1186/s12889-021-10530-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949240PMC
March 2021

Sex differences in habitat use, positional behavior, and gaits of Golden Snub-Nosed Monkeys (Rhinopithecus roxellana) in the Qinling Mountains, Shaanxi, China.

Primates 2021 May 10;62(3):507-519. Epub 2021 Mar 10.

Shaanxi Key Laboratory for Animal Conservation and College of Life Sciences, Northwest University, Xi'an, 710069, Shaanxi, China.

Studies of positional behavior, gait, and habitat use are important for understanding how animals adapt to the challenges of their environment. In turn, this information is useful for advancing research on primate morphology, life history, and ecology. Data on eco-mechanical variables can be used to develop concrete conservation and management plans for understudied and threatened primate groups. The present study explores the positional behavior, gaits, and habitat use of male and female adult golden snub-nosed monkeys (Rhinopithecus roxellana), an endemic, endangered, and highly dimorphic species of central China. Using focal animal sampling and opportunistic videorecording in the Guanyinshan National Nature Reserve on the southern slopes of the Qinling Mountains, it was determined that gait parameters were largely the same between sexes. By contrast, habitat use and, to a lesser extent, positional behavior varied significantly between males and females. In general, males were more terrestrial than females. When they moved arboreally, males also used a greater proportion of horizontal and large substrates compared to females. Furthermore, males used more standing postures, forelimb suspensory positional behaviors, and quadrupedal walking. These data suggest that, when faced with the mechanical challenges of large body size, primates such as R. roxellana are more likely to respond by altering habitat use rather than positional behaviors or intrinsic kinematics and timing.
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http://dx.doi.org/10.1007/s10329-021-00900-2DOI Listing
May 2021

Manganese-Dioxide-Coating-Instructed Plasmonic Modulation of Gold Nanorods for Activatable Duplex-Imaging-Guided NIR-II Photothermal-Chemodynamic Therapy.

Adv Mater 2021 Apr 1;33(13):e2008540. Epub 2021 Mar 1.

Marshall Laboratory of Biomedical Engineering, International Cancer Center, Laboratory of Evolutionary Theranostics, School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, 518060, China.

Nanotheranostic agents of gold nanomaterials in the second near-infrared (NIR-II) window have attracted significant attention in cancer management, owing to the reduced background signal and deeper penetration depth in tissues. However, it is still challenging to modulate the localized surface plasmon resonance (LSPR) of gold nanomaterials from the first near-infrared (NIR-I) to NIR-II region. Herein, a plasmonic modulation strategy of gold nanorods (GNRs) through manganese dioxide coating is developed for NIR-II photoacoustic/magnetic resonance (MR) duplex-imaging-guided NIR-II photothermal chemodynamic therapy. GNRs are coated with silica dioxide (SiO ) and then covered with magnesium dioxide (MnO ) to obtain the final product of [email protected] @MnO (denoted as GSM). The LSPR peak of GNRs could be tuned by adjusting the thickness of the MnO layer. Theoretical simulations reveal that this plasmonic modulation is mainly due to the change of refraction index around the GNRs after coating with the MnO layer. Additionally, the MnO layer is demonstrated to degrade into Mn ions in response to peroxide and acidic protons in the tumor microenvironment, which allows for MR imaging and chemodynamic therapy. This plasmonic modulation strategy can be adapted to other metal nanomaterials and the construction of a new class of NIR-II nanotheranostics.
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http://dx.doi.org/10.1002/adma.202008540DOI Listing
April 2021

Cryptobiosis-inspired assembly of "AND" logic gate platform for potential tumor-specific drug delivery.

Acta Pharm Sin B 2021 Feb 25;11(2):534-543. Epub 2020 Aug 25.

School of Food and Biological Engineering, School of Instrument Science and Opto-Electronics Engineering, Hefei University of Technology, Hefei 230009, China.

Developing tumor-specific drug delivery systems with minimized off-target cargo leakage remains an enduring challenge. In this study, inspired from the natural cryptobiosis explored by certain organisms and stimuli-responsive polyphenol‒metal coordination chemistry, doxorubicin (DOX)-conjugated gelatin nanoparticles with protective shells formed by complex of tannic acid and Fe ([email protected] NPs) were successfully developed as an "AND" logic gate platform for tumor-targeted DOX delivery. Moreover, benefiting from the well-reported photothermal conversion ability of TA-Fe complex, a synergistic tumor inhibition effect was confirmed by treating 4T1 tumor-bearing mice with [email protected] NPs and localized near-infrared (NIR) laser irradiation. As a proof of concept study, this work present a simple strategy for developing "AND" logic gate platforms by coating enzyme-degradable drug conjugates with detachable polyphenol‒metal shells.
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http://dx.doi.org/10.1016/j.apsb.2020.08.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893123PMC
February 2021

COVID-19 associated anxiety enhances tinnitus.

PLoS One 2021 5;16(2):e0246328. Epub 2021 Feb 5.

Department of Otolaryngology-Head and Neck Surgery, Sichuan Provincial People's Hospital and Sichuan Academy of Medical Sciences, Chengdu, Sichuan, China.

To investigate if the anxiety associated with coronavirus disease 2019 (COVID-19) is a promoting factor to tinnitus. A retrospective research design collected from 188 tinnitus patients, was used to compare the clinical characteristics of tinnitus between the patients in 2020 under pandemic pressure and those from the matching period in 2019. While anxiety was quantified using the Zung's Self-rating Anxiety Scale (SAS), tinnitus severity was evaluated using the Tinnitus Handicap Inventory (THI) questionnaire and the test of tinnitus loudness (TL). The assessments were repeated after the sound therapy plus educational counselling (STEC) for 38 patients in 2020 and 58 patients in 2019 and compared with EC alone therapy for 42 patients in 2020 and 17 patients in 2019. A large increase in anxiety was evident in 2020 in both case rate and SAS. The treatment of both methods was less effective in 2020. SAS, THI and TL were all deteriorated after the EC alone treatment in 2020, while an improvement was seen in 2019. This suggests that EC alone could not counteract the stress by COVID-19 at all, and the stress, if not managed well, can significantly increase the severity of tinnitus and associated anxiety. By using the EC subgroup in virtual control, we conclude that anxiety can serve as a promoting factor to tinnitus. We believe that this is the first study report that confirm the causative/promotive role of anxiety on tinnitus during COVID-19 pandemic.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246328PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864409PMC
February 2021

Does smoking impact trust in physicians and satisfaction with the health system in China?

Tob Induc Dis 2021 25;19:06. Epub 2021 Jan 25.

Department of General Psychology, College of Basic Medicine, Inner Mongolia Medical University, Hohhot, China.

Introduction: Trust and satisfaction play vital roles in how smokers react to smoking cessation treatment delivered by physicians. This paper aims to ascertain whether smoking status and pack-years of smoking affect trust in physicians and satisfaction with the health system in China.

Methods: The current study used the ordered probit model to evaluate how smoking status affects trust in physicians and satisfaction with the health system in China. Data from the China Family Panel Studies (CFPS) 2018 were used for the analysis, and the final sample consisted of 29500 adults. The CFPS is a nationally representative, comprehensive, high-quality, biennial longitudinal survey of Chinese communities, families, and individuals. The survey was conducted in 25 provinces and their administrative equivalents. The population of 25 provinces represents 95% of the total population in Mainland China.

Results: According to the ordered probit model, the results showed that current smokers were significantly negatively associated with trust in physicians, and more cigarette smoking was associated with decreased trust in physicians. Moreover, current smokers were also significantly negatively related to satisfaction with the health system.

Conclusions: The present study found that current smokers would be more likely to rate trust in physicians lower, and less likely to rate greater satisfaction with the health system, than never smokers. These results may have important implications for regaining trust in physicians from smokers and supporting health-system reform for tobacco treatment.
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http://dx.doi.org/10.18332/tid/131626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848684PMC
January 2021
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