Publications by authors named "He Cai"

106 Publications

Adaptive Deformation Control of a Flexible Variable-Length Rotary Crane Arm With Asymmetric Input-Output Constraints.

IEEE Trans Cybern 2021 Oct 6;PP. Epub 2021 Oct 6.

This article constructs two adaptive control laws to achieve deformation reduction and attitude tracking for a rotary variable-length crane arm with system parameter uncertainties and asymmetric input-output constraints. Two auxiliary systems are given to deal with the input constraints, an asymmetric-logarithm-barrier Lyapunov function is established for achieving the asymmetric output constrains, and five adaptive laws are constructed to handle system parameter uncertainties. Besides, the control design is based on a partial differential equation model, and the S-curve acceleration and deceleration method is used for regulating the arm extension speed. Both the system stability and uniform ultimate boundedness of the controlled crane arm are analyzed. Simulation results validate the effectiveness of our established control laws.
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http://dx.doi.org/10.1109/TCYB.2021.3112706DOI Listing
October 2021

Comparison of general and aesthetic effects between flapless and flap techniques in dental implantation: a meta-analysis of randomized controlled trials.

Int J Implant Dent 2021 10 1;7(1):100. Epub 2021 Oct 1.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, South Renmin Road, Chengdu, 610041, People's Republic of China.

Background: Information about the aesthetic effects of flapless in implant surgeries is scant. Differences of the survival rate (SR) and crestal bone loss (CBL) between the two techniques were also controversial. Thus, this review was aimed to compare the general and aesthetic effects of flapless and flap approaches in implant surgeries.

Materials And Methods: Following the principals of PRISMA, literature databases were searched for the eligible randomized controlled trials (RCTs) comparing the clinical performances of flap and flapless techniques. After that, relevant data of selected studies were pooled and analyzed to compare SR, bleeding on probing (BOP), probing depth (PD), visual analogue scale (VAS), papillae presentation index (PPI), keratinized mucosa (KM) width and CBL between the two techniques.

Results: Fourteen RCTs were included. No significant difference was found in SR (RR = - 0.01, 95% confidence interval (CI) (- 0.05, 0.04)), BOP (OR = 0.40, 95% CI (0.15, 1.02)), KM width (WMD = - 0.42, 95% CI (- 1.02, 0.17)) between two groups. Subgroup analysis revealed that the difference of CBL was insignificant in two groups (WMD = - 0.13, 95% CI (- 0.63, 0.38)). However, flap techniques would lead more peri-implant PD (WMD = - 0.37, 95% CI (- 0.51, - 0.23)). Subgroup analysis also indicated lower VAS scores in flapless group after 1 day (WMD = - 1.66, 95% CI (- 2.16, - 1.16)) but comparable pain experience after 3 days (WMD = - 0.59, 95% CI (- 1.33, 0.16)). Mean difference of PPI (WMD = 0.32, 95% CI (0.28, 0.35)) between the two groups was significant.

Conclusions: The flapless procedure showed a superiority in preserving gingival papillae, reducing postoperative pain and peri-implant PD compared to the flap procedure, while exhibiting comparable effects on SR, BOP, KW width changes and CBL. Flapless technique is more recommended at the ideal soft and hard tissue implanting sites.
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http://dx.doi.org/10.1186/s40729-021-00380-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484394PMC
October 2021

Prevalence of subtle distal Fallopian tube abnormalities and their association with endometriosis in infertility patients: a prospective cohort study.

Hum Fertil (Camb) 2021 Sep 27:1-6. Epub 2021 Sep 27.

Reproductive Medicine Center, Peking University People's Hospital, Beijing, China.

Subtle distal Fallopian tube abnormalities comprise a group of diseases that are characterised by subtle variations in tubal anatomy. This prospective cohort study investigated the prevalence of subtle distal Fallopian tube abnormalities in the infertile population and their relationship with endometriosis. It was conducted in a single fertility referral centre between January 2017 and December 2018 and included all infertile patients who underwent laparoscopy. Subtle distal Fallopian tube abnormalities included fimbrial agglutination, tubal diverticula, accessory ostium, fimbrial phimosis, and accessory Fallopian tube. A total of 876 patients were enrolled in the study, and 251 cases (28.65%; mean age: 29.4 ± 4.7 years) were diagnosed with subtle tube abnormalities. A total of 179 of these cases presented only one type of abnormality, 62 presented two types of abnormalities, and 12 presented three types. Tubal fimbrial agglutination composed the largest group (62.2%;  = 156), followed by tubal diverticula (26.3%;  = 66), fimbrial phimosis (25.5%;  = 64), tubal accessory ostium (15.5%;  = 39), and tubal accessory ostium (15.5%;  = 39). An accessory Fallopian tube was the least common abnormity (4.8%;  = 12). A total of 70.9% (178/251) of the women with subtle tubal abnormalities had endometriosis. The prevalence of subtle distal Fallopian tube abnormalities in the stage I-II group was significantly higher than in the stage III-IV group (57.3% [149/260] vs. 20.9% [29/139];  < 0.001). These findings indicate the high prevalence of subtle distal Fallopian tube abnormalities in the infertile woman. This group of diseases is highly related to endometriosis and may indicate fimbrial abnormalities of endometriosis.
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http://dx.doi.org/10.1080/14647273.2021.1981551DOI Listing
September 2021

Lymph-Node-Targeted Cholesterolized TLR7 Agonist Liposomes Provoke a Safe and Durable Antitumor Response.

Nano Lett 2021 10 17;21(19):7960-7969. Epub 2021 Sep 17.

Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, No. 17, Block 3, Southern Renmin Road, Chengdu, Sichuan 610041, PR China.

Toll-like receptor (TLR) agonists as the potent stimulants of an innate immune system hold promises for applications in anticancer immunotherapy. However, most of them are limited in the clinical translation due to the uncontrolled systemic inflammatory response. In the current study, 1V209, a small molecule TLR7 agonist, was conjugated with cholesterol (1V209-Cho) and prepared into liposomes (1V209-Cho-Lip). 1V209-Cho-Lip exerted minimal toxic effects and enhanced the transportation ability in lymph nodes (LNs) compared with 1V209. 1V209-Cho-Lip treatment inhibited tumor progression in CT26 colorectal cancer, 4T1 breast cancer, and Pan02 pancreatic ductal cancer models through inducing effective DC activation and eliciting CD8 T cell responses. Furthermore, 1V209-Cho-Lip induced tumor-specific memory immunity to inhibit cancer recurrence and metastasis. These results indicate that cholesterol conjugation with 1V209 is an effective approach to target lymph nodes and to reduce the adverse effects. This work provides a rational basis for the distribution optimization of TLR agonists for potential clinical use.
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http://dx.doi.org/10.1021/acs.nanolett.1c01968DOI Listing
October 2021

Circulating humanin is lower in coronary artery disease and is a prognostic biomarker for major cardiac events in humans.

Biochim Biophys Acta Gen Subj 2021 Sep 12:130010. Epub 2021 Sep 12.

The Cardiovascular Center, First Hospital of Jilin University, 71 Xinmin Road, Changchun, Jilin 130021, China. Electronic address:

Background: Humanin is an endogenous mitochondria-derived peptide that plays critical roles in oxidative stress, inflammation and CAD. In this study, we measured the levels of circulating humanin, markers of oxidative stress and inflammation in patients with unstable angina and MI and studied the relationship between these parameters and major adverse cardiac events (MACE).

Methods: A total of 327 subjects were recruited from the inpatient department at First Hospital of Jilin University and divided into 3 groups [control, angina and myocardial infarction (MI)] based on the clinical data and the results of the angiography. Serum humanin and thiobarbituric acid reactive substances (TBARS) were measured at the time of initial admission. The hospitalization data and MACE of all patients were collected.

Results: Circulating humanin levels were lower in the angina group compared to controls [124.22 ± 63.02 vs. 157.77 ± 99.93 pg/ml, p < 0.05] and even lower in MI patients [67.17 ± 24.35 pg/ml, p < 0.05 vs controls] and oxidative stress marker were higher in MI patients compared to the control and angina groups [12.94 ± 4.55 vs. 8.26 ± 1.66 vs. 9.06 ± 2.47 umol/ml, p < 0.05]. Lower circulating humanin levels was an independent risk factor of MI patients. Circulating humanin levels could be used to predict MACE in angina group.

Conclusions: Lower circulating humanin levels was an independent risk factor for CAD, and a potential prognostic marker for mild CAD.

General Significance: Humanin may become a new index for the diagnosis and treatment of CAD.
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http://dx.doi.org/10.1016/j.bbagen.2021.130010DOI Listing
September 2021

A single-cell atlas of liver metastases of colorectal cancer reveals reprogramming of the tumor microenvironment in response to preoperative chemotherapy.

Cell Discov 2021 Sep 7;7(1):80. Epub 2021 Sep 7.

Department of Pathology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China.

Metastasis is the primary cause of cancer-related mortality in colorectal cancer (CRC) patients. How to improve therapeutic options for patients with metastatic CRC is the core question for CRC treatment. However, the complexity and diversity of stromal context of the tumor microenvironment (TME) in liver metastases of CRC have not been fully understood, and the influence of stromal cells on response to chemotherapy is unclear. Here we performed an in-depth analysis of the transcriptional landscape of primary CRC, matched liver metastases and blood at single-cell resolution, and a systematic examination of transcriptional changes and phenotypic alterations of the TME in response to preoperative chemotherapy (PC). Based on 111,292 single-cell transcriptomes, our study reveals that TME of treatment-naïve tumors is characterized by the higher abundance of less-activated B cells and higher heterogeneity of tumor-associated macrophages (TAMs). By contrast, in tumors treated with PC, we found activation of B cells, lower diversity of TAMs with immature and less activated phenotype, lower abundance of both dysfunctional T cells and ECM-remodeling cancer-associated fibroblasts, and an accumulation of myofibroblasts. Our study provides a foundation for future investigation of the cellular mechanisms underlying liver metastasis of CRC and its response to PC, and opens up new possibilities for the development of therapeutic strategies for CRC.
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http://dx.doi.org/10.1038/s41421-021-00312-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421363PMC
September 2021

Harnessing biocompatible nanofibers and silver nanoparticles for wound healing: Sandwich wound dressing versus commercial silver sulfadiazine dressing.

Mater Sci Eng C Mater Biol Appl 2021 Sep 30;128:112342. Epub 2021 Jul 30.

Department of Cosmetic and Plastic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266071, China. Electronic address:

Owing to the structural replication of native extracellular matrix, nonwoven mats of electrospun nanofibers have great potential for use in wound healing. Herein, we report the design and fabrication of a sandwich wound dressing to balance its antimicrobial activity and biocompatibility. This success mainly relies on the incorporation of silver nanoparticles (AgNPs) into electrospun nanofibers, together with the rational design of a sandwich structure for the dressing. The bottom layer was composed of hydrophilic nanofibers made from a blend of polycaprolactone (PCL) and gelatin (Gel). The top layer consisted of hydrophobic PCL nanofibers. AgNP-loaded PCL/Gel nanofibers were sandwiched between the two layers. When compared with a commercial silver sulfadiazine dressing, the designed wound dressing showed competitive antimicrobial properties, lower cell toxicity, and accelerated wound closure for mouse skin injury. By balancing the biocompatibility of electrospun nanofibers and the broad-spectrum antibacterial activity of AgNPs within a sandwich structure, the novel multifunctional wound dressing could be valuable for effective wound healing and related applications.
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http://dx.doi.org/10.1016/j.msec.2021.112342DOI Listing
September 2021

Spontaneous apoptosis of cells in therapeutic stem cell preparation exert immunomodulatory effects through release of phosphatidylserine.

Signal Transduct Target Ther 2021 Jul 14;6(1):270. Epub 2021 Jul 14.

Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.

Mesenchymal stem cell (MSC)-mediated immunomodulation has been harnessed for the treatment of human diseases, but its underlying mechanism has not been fully understood. Dead cells, including apoptotic cells have immunomodulatory properties. It has been repeatedly reported that the proportion of nonviable MSCs in a MSC therapeutic preparation varied from 5~50% in the ongoing clinical trials. It is conceivable that the nonviable cells in a MSC therapeutic preparation may play a role in the therapeutic effects of MSCs. We found that the MSC therapeutic preparation in the present study had about 5% dead MSCs (DMSCs), characterized by apoptotic cells. Namely, 1 × 10 MSCs in the preparation contained about 5 × 10 DMSCs. We found that the treatment with even 5 × 10 DMSCs alone had the equal therapeutic effects as with 1 × 10 MSCs. This protective effect of the dead MSCs alone was confirmed in four mouse models, including concanavalin A (ConA)- and carbon tetrachloride (CCl)-induced acute liver injury, LPS-induced lung injury and spinal cord injury. We also found that the infused MSCs died by apoptosis in vivo. Furthermore, the therapeutic effect was attributed to the elevated level of phosphatidylserine (PS) upon the injection of MSCs or DMSCs. The direct administration of PS liposomes (PSLs) mimic apoptotic cell fragments also exerted the protective effects as MSCs and DMSCs. The Mer tyrosine kinase (MerTK) deficiency or the knockout of chemokine receptor C-C motif chemokine receptor 2 (CCR2) reversed these protective effects of MSCs or DMSCs. These results revealed that DMSCs alone in the therapeutic stem cell preparation or the apoptotic cells induced in vivo may exert the same immunomodulatory property as the "living MSCs preparation" through releasing PS, which was further recognized by MerTK and participated in modulating immune cells.
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http://dx.doi.org/10.1038/s41392-021-00688-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280232PMC
July 2021

A bivalent recombinant vaccine targeting the S1 protein induces neutralizing antibodies against both SARS-CoV-2 variants and wild-type of the virus.

MedComm (Beijing) 2021 May 17. Epub 2021 May 17.

Laboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics West China Hospital, Sichuan University Chengdu China.

The emerging variants of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in pandemic call for the urgent development of universal corona virus disease 2019 (COVID-19) vaccines which could be effective for both wild-type SARS-CoV-2 and mutant strains. In the current study, we formulated protein subunit vaccines with AS03 adjuvant and recombinant proteins of S1 subunit of SARS-CoV-2 (S1-WT) and S1 variant (K417N, E484K, N501Y, and D614G) subunit (S1-Mut), and immunized transgenic mice that express human angiotensin-converting enzyme 2 (hACE2). The S1 protein-specific antibody production and the neutralization capability for SARS-CoV-2 and B.1.351 variant were measured after immunization in mice. The results revealed that the S1-Mut antigens were more effective in inhibiting the receptor-binding domain and ACE2 binding in B.1.351 variant than in wild-type SARS-CoV-2. Furthermore, the development of a bivalent vaccine exhibited the ideal neutralization properties against wild-type and B.1.351 variant, as well as other variants. Our findings may provide a rationale for the development of a bivalent recombinant vaccine targeting the S1 protein that can induce the neutralizing antibodies against both SARS-CoV-2 variants and wild-type of the virus and may be of importance to explore the potential clinical use of bivalent recombinant vaccine in the future.
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http://dx.doi.org/10.1002/mco2.72DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242662PMC
May 2021

Protective Mechanism of Humanin Against Oxidative Stress in Aging-Related Cardiovascular Diseases.

Front Endocrinol (Lausanne) 2021 10;12:683151. Epub 2021 Jun 10.

The Cardiovascular Center, First Hospital of Jilin University, Changchun, China.

Physiological reactive oxygen species (ROS) are important regulators of intercellular signal transduction. Oxidative and antioxidation systems maintain a dynamic balance under physiological conditions. Increases in ROS levels destroy the dynamic balance, leading to oxidative stress damage. Oxidative stress is involved in the pathogenesis of aging-related cardiovascular diseases (ACVD), such as atherosclerosis, myocardial infarction, and heart failure, by contributing to apoptosis, hypertrophy, and fibrosis. Oxidative phosphorylation in mitochondria is the main source of ROS. Increasing evidence demonstrates the relationship between ACVD and humanin (HN), an endogenous peptide encoded by mitochondrial DNA. HN protects cardiomyocytes, endothelial cells, and fibroblasts from oxidative stress, highlighting its protective role in atherosclerosis, ischemia-reperfusion injury, and heart failure. Herein, we reviewed the signaling pathways associated with the HN effects on redox signals, including Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2), chaperone-mediated autophagy (CMA), c-jun NH2 terminal kinase (JNK)/p38 mitogen-activated protein kinase (p38 MAPK), adenosine monophosphate-activated protein kinase (AMPK), and phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)-Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3). Furthermore, we discussed the relationship among HN, redox signaling pathways, and ACVD. Finally, we propose that HN may be a candidate drug for ACVD.
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http://dx.doi.org/10.3389/fendo.2021.683151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222669PMC
June 2021

Sequencing and analysis of the complete mitochondrial genome of from China and its phylogenetic analysis.

Mitochondrial DNA B Resour 2021 May 12;6(5):1617-1620. Epub 2021 May 12.

College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, P.R. China.

The complete mitogenome sequence of was determined using long PCR. The genome was 16,238 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (33.7%), C (24.8%), T (29.1%) and G (12.4%). The base compositions present clearly the A-T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of in Muridae at the molecular level. The mitochondrial genome would be a significant supplement for the genetic background.
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http://dx.doi.org/10.1080/23802359.2021.1926353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118456PMC
May 2021

[Study on influence factors of acupoint function based on energetics].

Zhongguo Zhen Jiu 2021 May;41(5):521-4

College of Acupuncture-Moxibustion and Tuina, Guangxi University of CM, Nanning 530001, China.

Acupoint is the integration of structure and function. In this paper, the structure and function of acupoint are studied based on energetics. It is viewed that acupoint is the aggregation and release place of energy, acupuncture is the process of applying energy to acupoint, and the variations of speed, direction, time and local temperature are the key factors of acupoint function.
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http://dx.doi.org/10.13703/j.0255-2930.20200416-0006DOI Listing
May 2021

Adaptive Neural Network Control of a Flexible Spacecraft Subject to Input Nonlinearity and Asymmetric Output Constraint.

IEEE Trans Neural Netw Learn Syst 2021 May 17;PP. Epub 2021 May 17.

This article focuses on the vibration reducing and angle tracking problems of a flexible unmanned spacecraft system subject to input nonlinearity, asymmetric output constraint, and system parameter uncertainties. Using the backstepping technique, a boundary control scheme is designed to suppress the vibration and regulate the angle of the spacecraft. A modified asymmetric barrier Lyapunov function is utilized to ensure that the output constraint is never transgressed. Considering the system robustness, neural networks are used to handle the system parameter uncertainties and compensate for the effect of input nonlinearity. With the proposed adaptive neural network control law, the stability of the closed-loop system is proved based on the Lyapunov analysis, and numerical simulations are carried out to show the validity of the developed control scheme.
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http://dx.doi.org/10.1109/TNNLS.2021.3072907DOI Listing
May 2021

Access to 2-arylquinazolines catabolism/reconstruction of amino acids with the insertion of dimethyl sulfoxide.

Chem Commun (Camb) 2021 Jun;57(44):5414-5417

Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

Quinazoline skeletons are synthesized by amino acid catabolism/reconstruction combined with the insertion/cyclization of dimethyl sulfoxide for the first time. The amino acid acts as a carbon and nitrogen source through HI-mediated catabolism and is then reconstructed using aromatic amines and dimethyl sulfoxide (DMSO) as a one-carbon synthon. This protocol is of great significance for the further study of the conversion of amino acids.
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http://dx.doi.org/10.1039/d1cc00623aDOI Listing
June 2021

[Using old meshy cage cover to make a rat fixating device for acupuncture study].

Zhen Ci Yan Jiu 2021 Apr;46(4):348-50

College of Acupuncture-moxibustion and Tuina, Guangxi University of Chinese Medicine, Nanning 530001, China; Institute of Acupuncture and Moxibustion, Guangxi University of Chinese Medicine, Nanning 530001.

Binding-fixation is the most commonly used method for acupuncture research in rats, but its resulted stronger stress response may affect the experimental outcomes. Hence, we developed a practical fixing device using the abandoned meshy cage covers, bolts, hooks, and loop fasteners. It includes two straps, two ropes, a small jacket with a self-locking Velcro strap and a meshy cage cover with a piece of fixed Velcro which may be placed on a rectangular storage box. Rats can be fixed in a recumbent position or a prone position. Outcomes showed that it is not only convenient and cheaper but also has a higher successful rate of fixation and a longer fixation time for completing an experiment.
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http://dx.doi.org/10.13702/j.1000-0607.200433DOI Listing
April 2021

Asymmetric Input-Output Constraint Control of a Flexible Variable-Length Rotary Crane Arm.

IEEE Trans Cybern 2021 Apr 20;PP. Epub 2021 Apr 20.

This article demonstrates the realization of angle tracking and deformation suppression by developing two boundary controllers for a flexible variable-length rotary crane arm with extraneous disturbances and asymmetric input-output constraints. The dynamic model description of this kind of crane arm system is several partial differential equations integrated into few ordinary differential equations. The S-curve acceleration and deceleration scheme is utilized to adjust the elongation rate of the arm. A kind of novel observer is put forward to tackle unknown extraneous disturbances. Auxiliary systems and barrier Lyapunov functions are introduced to meet the asymmetric input-output constraints. With the help of Lyapunov's theory, the global exponential stability and uniform boundedness are analyzed. The numerical simulations are finally provided to illuminate its availability of the designed control schemes.
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http://dx.doi.org/10.1109/TCYB.2021.3055151DOI Listing
April 2021

Effects of immediate and delayed loading protocols on marginal bone loss around implants in unsplinted mandibular implant-retained overdentures: a systematic review and meta-analysis.

BMC Oral Health 2021 03 17;21(1):122. Epub 2021 Mar 17.

Department of Prosthodontics, School & Hospital of Stomatology, Tianjin Medical University, Tianjin, 300070, China.

Background: Immediate loading has recently been introduced into unsplinted mandibular implant-retained overdentures for the management of edentulous patients due to their increasing demand on immediate aesthetics and function. However, there is still a scarcity of meta-analytical evidence on the efficacy of immediate loading compared to delayed loading in unsplinted mandibular implant-retained overdentures. The purpose of this study was to compare the marginal bone loss (MBL) around implants between immediate and delayed loading of unsplinted mandibular implant-retained overdentures.

Methods: Randomized controlled trials (RCTs), controlled clinical trials (CCTs), and cohort studies quantitatively comparing the MBL around implants between immediate loading protocol (ILP) and delayed loading protocol (DLP) of unsplinted mandibular overdentures were included. A systematic search was carried out in PubMed, EMBASE, and CENTRAL databases on December 02, 2020. "Grey" literature was also searched. A meta-analysis was conducted to compare the pooled MBL of two different loading protocols of unsplinted mandibular overdentures through weighted mean differences (WMDs) with 95% confidence intervals (95% CIs). The subgroup analysis was performed between different attachment types (i.e. Locator attachment vs. ball anchor). The risk of bias within and across studies were assessed using the Cochrane Collaboration's tool, the Newcastle-Ottawa scale, and Egger's test.

Results: Of 328 records, five RCTs and two cohort studies were included and evaluated, which totally contained 191 participants with 400 implants. The MBL of ILP group showed no significant difference with that of DLP group (WMD 0.04, CI - 0.13 to 0.21, P > .05). The subgroup analysis revealed similar results with Locator attachments or ball anchors (P > .05). Apart from one RCT (20%) with a high risk of bias, four RCTs (80%) showed a moderate risk of bias. Two prospective cohort studies were proved with acceptable quality. Seven included studies have reported 5.03% implant failure rate (10 of 199 implants) in ILP group and 1.00% failure rate (2 of 201 implants) in DLP group in total.

Conclusions: For unsplinted mandibular implant-retained overdentures, the MBL around implants after ILP seems comparable to that of implants after DLP. Immediate loading may be a promising alternative to delayed loading for the management of unsplinted mandibular implant-retained overdentures. PROSPERO registration number: CRD42020159124.
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http://dx.doi.org/10.1186/s12903-021-01486-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968211PMC
March 2021

Wrapping the stump of the gastroduodenal artery using the ligamentum teres hepatis during laparoscopic pancreaticoduodenectomy: a center's preliminary experience.

BMC Surg 2021 Feb 2;21(1):70. Epub 2021 Feb 2.

Department of Pancreatic Surgery, West China Hospital of Sichuan University, No. 37, Guoxue Alley, Chengdu, 610041, Sichuan, China.

Background: The present study aims to assess the preliminary outcomes of the effectiveness of wrapping the ligamentum teres hepatis (LTH) around the gastroduodenal artery stump for the prevention of erosion hemorrhage after laparoscopic pancreaticoduodenectomy (LPD).

Methods: We reviewed 247 patients who had undergone LPD between January 2016 and April 2019. The patients were divided into two groups according to whether LTH wrapped the stump of the gastroduodenal artery: group A (119 patients) who underwent the LTH wrapping procedure, and group B (128 patients) who did not undergo the procedure. The perioperative data from the two groups were reviewed to assess the effectiveness of the LTH procedure for the prevention of postpancreatectomy hemorrhage (PPH) and other complications.

Results: No differences were observed in the clinical characteristics between the two groups. The data from 247 patients were acceptable for analysis: 119 patients underwent wrapping, and 128 patients did not. The incidence of clinically relevant pancreatic fistula (8.4% vs 3.9%), biliary fistula (2.5% vs 1.6%), intra-abdominal infection (10.1% vs 3.9%) and delayed gastric emptying (13.4% vs 16.4%) showed no significant difference between group A and group B. The 90-day mortality and 90-day reoperation rates (0.8% vs 0.8% and 5.0% vs 3.1%) were also similar between group A and group B. Furthermore, postpancreatectomy hemorrhage of Grade B and C occurred in 0 patients (0.0%) in the wrapping group, which was significantly less frequent than the occurrence in the nonwrapping group (7 patients; 5.5%, P = 0.02).

Conclusions: Wrapping the LTH around the gastroduodenal artery stump after LPD does not reduce the incidence of clinically relevant pancreatic fistula, biliary fistula or delayed gastric emptying. However, this procedure has a trend of reducing the rate of PPH of Grade B and C after LPD and is simple to perform.
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http://dx.doi.org/10.1186/s12893-021-01076-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856715PMC
February 2021

Dynamic prediction for clinically relevant pancreatic fistula: a novel prediction model for laparoscopic pancreaticoduodenectomy.

BMC Surg 2021 Jan 4;21(1). Epub 2021 Jan 4.

Department of Pancreatic Surgery, West China Hospital, Sichuan University, No. 37, Guoxue Alley, Chengdu, 610041, Sichuan Province, China.

Background: With the recent emerge of dynamic prediction model on the use of diabetes, cardiovascular diseases and renal failure, and its advantage of providing timely predicted results according to the fluctuation of the condition of the patients, we aim to develop a dynamic prediction model with its corresponding risk assessment chart for clinically relevant postoperative pancreatic fistula after laparoscopic pancreaticoduodenectomy by combining baseline factors and postoperative time-relevant drainage fluid amylase level and C-reactive protein-to-albumin ratio.

Methods: We collected data of 251 patients undergoing LPD at West China Hospital of Sichuan University from January 2016 to April 2019. We extracted preoperative and intraoperative baseline factors and time-window of postoperative drainage fluid amylase and C-reactive protein-to-albumin ratio relevant to clinically relevant pancreatic fistula by performing univariate and multivariate analyses, developing a time-relevant logistic model with the evaluation of its discrimination ability. We also established a risk assessment chart in each time-point.

Results: The proportion of the patients who developed clinically relevant postoperative pancreatic fistula after laparoscopic pancreaticoduodenectomy was 7.6% (19/251); preoperative albumin and creatine levels, as well as drainage fluid amylase and C-reactive protein-to-albumin ratio on postoperative days 2, 3, and 5, were the independent risk factors for clinically relevant postoperative pancreatic fistula. The cut-off points of the prediction value of each time-relevant logistic model were 14.0% (sensitivity: 81.9%, specificity: 86.5%), 8.3% (sensitivity: 85.7%, specificity: 79.1%), and 7.4% (sensitivity: 76.9%, specificity: 85.9%) on postoperative days 2, 3, and 5, respectively, the area under the receiver operating characteristic curve was 0.866 (95% CI 0.737-0.996), 0.896 (95% CI 0.814-0.978), and 0.888 (95% CI 0.806-0.971), respectively.

Conclusions: The dynamic prediction model for clinically relevant postoperative pancreatic fistula has a good to very good discriminative ability and predictive accuracy. Patients whose predictive values were above 14.0%, 8.3%, and 7.5% on postoperative days 2, 3, and 5 would be very likely to develop clinically relevant postoperative pancreatic fistula after laparoscopic pancreaticoduodenectomy.
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http://dx.doi.org/10.1186/s12893-020-00968-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784027PMC
January 2021

Potential roles of mediator Complex Subunit 13 in Cardiac Diseases.

Int J Biol Sci 2021 1;17(1):328-338. Epub 2021 Jan 1.

Pediatric Research Institute, the Department of Pediatrics of University of Louisville, Louisville, KY 40202, USA.

Mediator complex subunit 13 (MED13, previously known as THRAP1 and TRAP240) is a subunit of the cyclin-dependent kinase 8 (CDK8) kinase module in the eukaryotic mediator complex. MED13 has been known to play critical roles in cell cycle, development, and growth. The purpose of this review is to comprehensively discuss its newly identified potential roles in myocardial energy metabolism and non-metabolic cardiovascular diseases. Evidence indicates that cardiac MED13 mainly participates in the regulation of nuclear receptor signaling, which drives the transcription of genes involved in modulating cardiac and systemic energy homeostasis. MED13 is also associated with several pathological conditions, such as metabolic syndrome and thyroid disease-associated heart failure. Therefore, MED13 constitutes a potential therapeutic target for the regulation of metabolic disorders and other cardiovascular diseases.
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http://dx.doi.org/10.7150/ijbs.52290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757031PMC
January 2021

A mouse model for SARS-CoV-2-induced acute respiratory distress syndrome.

Signal Transduct Target Ther 2021 01 1;6(1). Epub 2021 Jan 1.

Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, No. 17, Block 3, Southern Renmin Road, 610041, Chengdu, Sichuan, People's Republic of China.

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http://dx.doi.org/10.1038/s41392-020-00451-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775436PMC
January 2021

Cationic nanocarriers as potent adjuvants for recombinant S-RBD vaccine of SARS-CoV-2.

Signal Transduct Target Ther 2020 12 11;5(1):291. Epub 2020 Dec 11.

Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, 610041, Sichuan, Chengdu, China.

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http://dx.doi.org/10.1038/s41392-020-00434-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729145PMC
December 2020

3D printing of metal-organic framework incorporated porous scaffolds to promote osteogenic differentiation and bone regeneration.

Nanoscale 2020 Dec;12(48):24437-24449

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, PR China.

Bone substitute biomaterials, whose compositions and structures both play vital roles in bone defect healing, hold promising prospects in the clinical treatment of bone defects. Three-dimensional (3D) printed porous scaffolds incorporating osteoinductive components are considered as ideal bone grafts, because of the accurate control of structure parameters and the capacity to enhance bone tissue regeneration. Our previous studies demonstrated that nanoscale zeolitic imidazolate framework-8 (nanoZIF-8), a subclass of metal organic frameworks (MOFs), presented minimal cytotoxicity, inhibited bacterial activities, and promoted osteogenesis both in vitro and in vivo. However, the application of nanoZIF-8 in the 3D printed scaffold system remains unknown. In this study, nanoZIF-8 was incorporated into composite scaffolds composed of polycaprolactone (PCL) and dicalcium phosphate dihydrate (DCPD) via extrusion-based 3D printing technology. The results revealed that the composite scaffolds possessed increased mechanical strength and exhibited homogeneous structure with highly interconnected macropores. In vitro studies indicated that scaffolds showed biocompatibility to bone mesenchymal stem cells (BMSCs), significantly up-regulated the expression of osteogenesis-related genes and proteins, and facilitated the extracellular matrix mineralization. In vivo results showed that 3D printed porous scaffolds promoted new bone formation and enhanced micro-architecture at the critical defect sites (Φ10 mm) in rabbits, compared with the blank control group. Moreover, composite scaffolds significantly improved calvarial defect healing in comparison with scaffolds without nanoZIF-8 incorporation. In summary, nanoZIF-8 modified 3D printed porous composite scaffolds demonstrated great potential in the treatment of critical-sized bone defects, proving the effectiveness of MOF incorporation in 3D printed composite scaffolds to promote osteogenesis in the field of bone tissue engineering.
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http://dx.doi.org/10.1039/d0nr06297aDOI Listing
December 2020

Palladium catalyzed annulation of 2-iodobiphenyl with a non-terminal alkene enabled by neighboring group assistance.

Chem Commun (Camb) 2021 Jan;57(1):121-124

Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

In this work, an efficient palladium catalyzed annulation of 2-iodobiphenyl with a non-terminal alkene was developed. The key factor in this transformation was the formation of a highly reactive oxo-palladacycle intermediate, which was enabled by a neighboring hydroxyl group, and remarkably restrained the β-H elimination process. Mechanistically, control experiments demonstrated that the hydroxyl group may act as an anionic ligand, which was irreplaceable in this reaction. This transformation presented good reactivity and selectivity, and no simple Heck coupling products were detected for all of the explored substrates.
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http://dx.doi.org/10.1039/d0cc07133aDOI Listing
January 2021

Prognostic factors for finger interphalangeal joint osteoarthritis: a systematic review.

Rheumatology (Oxford) 2021 03;60(3):1080-1090

Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.

Objective: Radiographic hand OA affects one in five adults. Symptomatic hand OA can result in functional impairment, pain and reduced quality of life. A prevalent form of hand OA is IP joint OA, however prognostic factors for IP joint OA remain poorly understood. This systematic review aimed to identify prognostic factors for IP joint OA, and to summarize the diagnostic criteria for IP joint OA in prognostic studies.

Methods: EMBASE, MEDLINE, Scopus and The Cochrane Library were searched from inception until 19 February 2020 (PROSPERO CRD42019116782). Eligible studies described diagnostic criteria defining IP joint OA, and assessed potential prognostic factors for IP joint OA. Risk of bias was assessed using a modified Quality in Prognosis Studies (QUIPS) tool and a best evidence synthesis was used.

Results: Eighteen studies were included (risk of bias: eight high, three moderate, seven low). All defined OA radiographically, and three studies incorporated clinical symptoms into their definition of OA. Forty-nine potential prognostic factors were assessed. Eight were prognostic: older age in women, female gender (both moderate evidence); family history of Heberden's nodes, Kashin-Beck disease, older age in men, dental occupation in men, finger fracture, parity (all limited evidence). Higher BMI in women (limited evidence) was prognostic for symptomatic radiographic OA. No prognostic factors for symptomatic OA were identified.

Conclusion: IP joint OA is most commonly defined radiographically, yet criteria were heterogeneous. Eight prognostic factors for radiographic IP joint OA and one for symptomatic radiographic IP joint OA were identified, all with limited or moderate evidence. Further studies on causality and on prognostic pathways are needed.
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http://dx.doi.org/10.1093/rheumatology/keaa735DOI Listing
March 2021

The regulatory roles of p53 in cardiovascular health and disease.

Cell Mol Life Sci 2021 Mar 11;78(5):2001-2018. Epub 2020 Nov 11.

Department of Pediatrics, Pediatric Research Institute, University of Louisville, Louisville, KY, 40202, USA.

Cardiovascular disease (CVD) remains the leading cause of mortality globally, so further investigation is required to identify its underlying mechanisms and potential targets for its prevention. The transcription factor p53 functions as a gatekeeper, regulating a myriad of genes to maintain normal cell functions. It has received a great deal of research attention as a tumor suppressor. In the past three decades, evidence has also shown a regulatory role for p53 in the heart. Basal p53 is essential for embryonic cardiac development; it is also necessary to maintain normal heart architecture and physiological function. In pathological cardiovascular circumstances, p53 expression is elevated in both patient samples and animal models. Elevated p53 plays a regulatory role via anti-angiogenesis, pro-programmed cell death, metabolism regulation, and cell cycle arrest regulation. This largely promotes the development of CVDs, particularly cardiac remodeling in the infarcted heart, hypertrophic cardiomyopathy, dilated cardiomyopathy, and diabetic cardiomyopathy. Roles for p53 have also been found in atherosclerosis and chemotherapy-induced cardiotoxicity. However, it has different roles in cardiomyocytes and non-myocytes, even in the same model. In this review, we describe the different effects of p53 in cardiovascular physiological and pathological conditions, in addition to potential CVD therapies targeting p53.
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http://dx.doi.org/10.1007/s00018-020-03694-6DOI Listing
March 2021

Quadruple C-H activation coupled to hydrofunctionalization and C-H silylation/borylation enabled by weakly coordinated palladium catalyst.

Nat Commun 2020 11 9;11(1):5662. Epub 2020 Nov 9.

Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, PR China.

Unlike the well-reported 1,2-difunctionalization of alkenes that is directed by classic pyridine and imine-containing directing groups, oxo-palladacycle intermediates featuring weak Pd-O coordination have been less demonstrated in C-H activated cascade transformations. Here we report a quadruple C-H activation cascade as well as hydro-functionalization, C-H silylation/borylation sequence based on weakly coordinated palladium catalyst. The hydroxyl group modulates the intrinsic direction of the Heck reaction, and then acts as an interrupter that biases the reaction away from the classic β-H elimination and toward C-H functionalization. Mechanistically, density functional theory calculation provides important insights into the key six-membered oxo-palladacycle intermediates, and indicates that the β-H elimination is unfavorable both thermodynamically and kinetically. In this article, we focus on the versatility of this approach, which is a strategic expansion of the Heck reaction.
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http://dx.doi.org/10.1038/s41467-020-19508-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652853PMC
November 2020

Early and late pregnancy loss in women with polycystic ovary syndrome undergoing IVF/ICSI treatment: a retrospective cohort analysis of 21 820 pregnancies.

BJOG 2021 06 19;128(7):1160-1169. Epub 2020 Nov 19.

Assisted Reproduction Centre, Northwest Women and Children's Hospital, Xi'an, China.

Objective: To examine early and late pregnancy loss in women with and without polycystic ovary syndrome (PCOS) undergoing IVF/ICSI transfers.

Design: Retrospective cohort study.

Setting: Reproductive medicine centre at a tertiary hospital.

Population: We studied women with a positive β-human chorionic gonadotropin (β-hCG) after in vitro fertilisation/intra-cytoplasmic sperm injection (IVF/ICSI) treatment from May 2014 to April 2019.

Methods: Odds ratios (OR) for early (≤13 weeks) and late (>13 weeks) pregnancy loss were calculated among women with and without PCOS for plurality of the pregnancy with adjustment for confounding factors.

Main Outcome Measures: Early pregnancy loss (EPL) and late pregnancy loss (LPL).

Results: From 21 820 women identified with a positive β-hCG, 2357 (10.8%) women had PCOS, and 19 463 (89.2%) women did not. EPL occurred in 16.6% (391) of women with PCOS versus 18.3% (3565) in women with non-PCOS (OR 0.89, 95% CI 0.79-0.99, P = 0.04). After adjustment for age and other confounders, the rate of EPL was not statistically significantly associated with PCOS status (adjusted OR [aOR] 0.91, 95% CI 0.80-1.05). Women with PCOS demonstrated a higher rate of LPL (6.4% in PCOS versus 3.6% in non-PCOS, OR 1.81, 95% CI 1.48-2.21, P < 0.001). In multivariable analysis, the potential impact of PCOS was less strong (aOR 1.38, 95% CI 0.96-1.98), with BMI and maternal comorbidities also associated with LPL (aOR 1.08, 95% CI 1.04-1.1 and aOR 2.07, 95% CI 1.43-3.00, respectively).

Conclusions: Polycystic ovary syndrome was not independently associated with EPL. There was an increased risk of LPL but this difference was not statistically significant.

Tweetable Abstract: Polycystic ovary syndrome women are at increased risk of late pregnancy loss, partly driven by elevated BMI and maternal comorbidities.
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http://dx.doi.org/10.1111/1471-0528.16590DOI Listing
June 2021

Rongalite as a sulfone source: a novel copper-catalyzed sulfur dioxide anion incorporation process.

Chem Commun (Camb) 2020 Nov 16;56(88):13653-13656. Epub 2020 Oct 16.

Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

A novel copper-catalyzed sulfur dioxide anion incorporation cascade for the synthesis of 1-thiaflavanone sulfones has been disclosed using rongalite as an economic and safe sulfone source. A series of 1-thiaflavanone sulfones were synthesized from easily prepared 2'-iodochalcone derivatives in excellent yields. This transformation proceeds through consecutive formation of two C-S bonds, which is the first example of SO being used to construct sulfone motifs under copper-catalyzed conditions.
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http://dx.doi.org/10.1039/d0cc05800aDOI Listing
November 2020

Pharmacological Regulation of Primary Cilium Formation Affects the Mechanosensitivity of Osteocytes.

Calcif Tissue Int 2020 12 17;107(6):625-635. Epub 2020 Sep 17.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, No. 37 Xueyuan Road, Haidian District, Beijing, 100083, China.

Primary cilia are responsible for sensing mechanical loading in osteocytes. However, the underlying working mechanism of cilia remains elusive. An osteocyte model is necessary to reveal the role of cilia. Furthermore, the osteocyte model should be with upregulated or downregulated primary cilium expression. Herein, we used a pharmacological method to regulate the cilium formation of osteocytes. After screening, some pharmacological agents can regulate the cilium formation of osteocytes. We performed a CCK-8 assay to analyze the optimal working conditions of the drugs for MLO-Y4 cells. The agents include chloral hydrate (CH), Gd, Li, and rapamycin. The expression of cilia affects the cellular functions, including mechanosensitivity, of osteocytes. Results showed that CH downregulated the cilium formation and ciliogenesis of osteocytes. In addition, Gd, Li, and rapamycin upregulated the cilium expression of osteocytes. Moreover, the cilium expression positively correlated with the mechanosensitivity of osteocytes. This work reveals the role of primary cilia in the mechanosensing of osteocytes.
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http://dx.doi.org/10.1007/s00223-020-00756-6DOI Listing
December 2020
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