Hassan Zackaria Ali Ishag

Dr Hassan Zackaria Ali Ishag

BSc, MSc, PhD

College of Veterainry Sceinses, University of Nyala, Sudan

Assistant Professor

Nyala, South Darfur State | Sudan

Specialties: Molecular Virology/Bacteriology

Hassan Zackaria Ali Ishag

Dr Hassan Zackaria Ali Ishag

BSc, MSc, PhD
Introduction

Dr. Hassan Z. A. Ishag is an Assistant Professor in the Department of Veterinary Clinical Studies at the University of Nyala, Nyala, Sudan. He graduated with a BSc Degree in Veterinary Sciences, Collge of Veterinary Sciences, University of Nyala, Sudan in 2003. He obtained his MSc Degree in Molecular Medicine, Institute of Endemic Diseases (IEND), University of Khartoum, Sudan in 2008. His Research was Molecular Detection and Characterization of Bovine Tuberculosis in Sudan. He obtained his Ph. D from the Key Laboratory of Animal Infectious Diseases, Diagnosis and Control, Nanjing Agricultural University, China. His Ph. D research was in Antiviral therapy Research. Currently, Dr. Ali has been working for his post-doctorate at the Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences (JAAS), Key Laboratory of Veterinary Biological Engineering and Technology, National Research Center for Engineering and Technology of Veterinary Bio-products, Nanjing , China. He He is leading a research projects in the development of genetic tools for functional genomic analysis.

Primary Affiliation: College of Veterainry Sceinses, University of Nyala, Sudan - Nyala, South Darfur State , Sudan

Specialties:

Research Interests:

Metrics

24

Publications

135

Profile Views

70

Reads

74

PubMed Central Citations

Top co-authors
Xiang Mao
Xiang Mao

College of Veterinary Medicine

10
Chen Li
Chen Li

Catalytic Hydrogenation Research Center

8
Li Huang
Li Huang

Guangxi Key Laboratory of Veterinary Biotechnology

8
Qiyan Xiong
Qiyan Xiong

China Pharmaceutical University

4
Ming-Xia Sun
Ming-Xia Sun

College of Veterinary Medicine

4
Bin Zhou
Bin Zhou

The State Key Laboratory of Cell Biology

4
Maojun Liu
Maojun Liu

Institute of Veterinary Medicine

4
Zhixin Feng
Zhixin Feng

College of Veterinary Medicine

4
Guoqing Shao
Guoqing Shao

Huazhong Agricultural University

4
Ming-Xia Sun
Ming-Xia Sun

College of Veterinary Medicine

3

Publications

24Publications

70Reads

74PubMed Central Citations

Development of oriC-plasmids for use in Mycoplasma hyorhinis.

Sci Rep 2017 Sep 6;7(1):10596. Epub 2017 Sep 6.

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products, Nanjing, 210014, China.

View Article
September 2017
1 Read
5.08 Impact Factor

E. coli recA gene improves gene targeted homologous recombination in Mycoplasma hyorhinis.

J Microbiol Methods 2017 05 8;136:49-56. Epub 2017 Mar 8.

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products, Nanjing 210014, China.

View Article
May 2017
3 Reads
1 PubMed Central Citation(source)
2.03 Impact Factor

GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis.

Springerplus 2016 17;5(1):769. Epub 2016 Jun 17.

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products, Nanjing, 210014 China.

View Article
July 2016
6 Reads
1 PubMed Central Citation(source)

In vitro protective efficacy of Lithium chloride against Mycoplasma hyopneumoniae infection.

Res Vet Sci 2016 Jun 4;106:93-6. Epub 2016 Apr 4.

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products, Nanjing 210014, China. Electronic address:

View Article
June 2016
1 Read
1.41 Impact Factor

Griffithsin binds to the glycosylated proteins (E and prM) of Japanese encephalitis virus and inhibit its infection.

Virus Res 2016 Apr 25;215:50-4. Epub 2016 Jan 25.

College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, Jiangsu Province, China; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China. Electronic address:

View Article
April 2016
6 Reads
3 PubMed Central Citations(source)
2.32 Impact Factor

A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae.

Genet Mol Res. 2016 Apr 28;15(2). doi: 10.4238/gmr.15027832.

Genet Mol Res

Mycoplasma hyopneumoniae (M. hyopneumoniae) causes porcine enzootic pneumonia (PEP) that significantly affects the pig industry worldwide. Despite the availability of the whole genome sequence, studies on the pathogenesis of this organism have been limited due to the lack of a genetic manipulation system. Therefore, the aim of the current study was to generate a general GFP reporter vector based on a replicating plasmid. Here, we describe the feasibility of GFP reporter expression in M. hyopneumoniae (strain 168L) controlled by the p97 gene promoter of this mycoplasma. An expression plasmid (pMD18-TOgfp) containing the p97 gene promoter, and origin of replication (oriC) of M. hyopneumoniae, tetracycline resistant marker (tetM), and GFP was constructed and used to transform competent M. hyopneumoniae cells. We observed green fluorescence in M. hyopneumoniae transformants under fluorescence microscopy, which indicates that there was expression of the GFP reporter that was driven by the p97 gene promoter. Additionally, an electroporation method for M. hyopneumoniae with an efficiency of approximately 1 x 10(-6) transformants/μg plasmid DNA was optimized and is described herein. In conclusion, our data demonstrate the susceptibility of M. hyopneumoniae to genetic manipulation whereby foreign genes are expressed. This work may encourage the development of genetic tools to manipulate the genome of M. hyopneumoniae for functional genomic analyses.

View Article
April 2016
2 Reads

Monoclonal Antibodies Against Porcine sIgA and Their Use in Immunohistochemistry.

Monoclon Antib Immunodiagn Immunother 2015 Dec;34(6):386-9

1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .

View Article
December 2015
1 Read

The establishment of a swine model with high virulence organization of Mycoplasma pneumonia

J Agri. Uni. Hebei. 2015, (6) 79-82+87

Journal of Agricultural University of Hebei

This study was designed to develop swine infection model with a high virulence pathogen of Mycoplasma pneumonia(Mhp).The level of the infected pathogen was quantified by qPCR and the safety of the pathogen used to establish the infection model was analyzed.The pigs were infected with high virulence Mhp by intra-tracheal ways at 1:10,1:100,1:1 000 and 1:10 000 dilutions.On the day 28 post-infection,the infected pigs were slaughtered and level of lung lesion and clinical symptoms from different dilution groups were compared.We found that the pigs will be infected when inoculated intra-tracheal with high virulence Mhp at a titer of 9.50×10~4.Herein,we established an infection swine infection model with high virulence Mhp which might be useful for the future research of Mhp.

View Article
December 2015
2 Reads

Preparation of the porcine secretory component and a monoclonal antibody against this protein.

Protein Expr Purif 2015 Sep 8;113:51-5. Epub 2015 May 8.

Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

View Article
September 2015
3 Reads
1.70 Impact Factor

The Effect of Complexed Zinc and Manganese in Performance, Skin Quality and Meat Quality Of Broilers: A review Article.

Veterinaeria, 2015; 3(2)1-4

Veterinaeria

Trace minerals such as zinc, copper, and manganese are essential cofactors for hundreds of cellular enzymes and transcription factors in all animal species. Thus, trace minerals participate in several biochemical processes such as immune development and response, tissue and bone development and integrity, protection against oxidative stress, and cellular growth and division. T race minerals are critical for a wide variety of physiological processes in all animals. Besides, Trace minerals act as cofactors of enzymes which are important to the immunity of animal. Superoxide dismutase, glutathione reductase, glutathione peroxidase, thioredoxin reductase, ceruloplasmin and catalase are important enzymes that have trace elements as cofactors. These enzymes act as antioxidants and prevent oxidative stress by neutralizing oxidants produced under different stresses. Zinc (Zn) and Manganese (Mn) are important trace minerals in animal nutrition involved in many metabolic processes, including growth, skin quality and wound healing. Bioavailability of these trace mineral can be improved through complexing them with available compounds that can enhance mineral absorption. Zn complexed with organic compounds showed better absorption in animals than inorganic Zn sources. Overall, trace minerals improve competence and productive performance and meat quality

View Article
May 2015
4 Reads

Molecular detection of bovine tuberculosis in El Rank area, North Upper Nile State, Sudan

Sci Let. 2015 3(1) 23-26.

Science Lettters

Bovine tuberculosis (TB) is an infectious disease of cattle and one of the biggest challenges in the cattle industry. It is caused mainly by the bacterium Mycobacterium bovis (M. bovis), which can also infect and cause TB in other mammals. In this study, we aimed to investigate the presence ofthe bovine tuberculosis in the El Rank area, northupper Nile State, Sudan. The semi-structured questionnaire, conventional methods (Ziehl’s-Neelsen staining, culture in Lowenstein-Jensen medium) and polymerase chain reaction (PCR) were used to detect the bovine tuberculosis in the El Rank area. T he results of the semi-structured questionnaire indicated that the Kenana cattle were at a high risk of infection (50%),especially the females around the age of 3 years. For the conventional methods and PCR, a total of forty milk samples and thirty aspirated lymph node samples were investigated for the presence of the bacterium. Only 1/30 (3.3%) of lymph node samples showed the characteristic appearance of the acid fast bacilli of a serpentine cord of M. bovis. This sample also showed a growth in the Lowenstein-Jensen medium containing pyruvate. The PCR results to amplify the rpoBgene (235bp) of M. bovisshowed a high sensitivity to detect the bacterium. It confirmed the presence of M. bovisin 26 (65%) of milk samples and 12 (40%) of lymph node samples. Our results confirmed the presence of M. bovisin El Rank area, north upper Nile State, Sudan andit also indicated the sensitivity of the PCR as a rapid tool to detect the infection of M. bovis

View Article
January 2015
3 Reads

A tripeptide (NSK) inhibits Japanese encephalitis virus infection in vitro and in vivo.

Arch Virol 2014 May 30;159(5):1045-55. Epub 2013 Nov 30.

College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, Jiangsu, China.

View Article
May 2014
4 Reads
2.28 Impact Factor

Cellular DDX3 regulates Japanese encephalitis virus replication by interacting with viral un-translated regions.

Virology 2014 Jan 26;449:70-81. Epub 2013 Nov 26.

College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, Jiangsu Province 210095, China. Electronic address:

View Article
January 2014
8 Reads
11 PubMed Central Citations(source)
3.32 Impact Factor

The DEAD-box RNA helicase DDX5 acts as a positive regulator of Japanese encephalitis virus replication by binding to viral 3' UTR.

Antiviral Res 2013 Nov 12;100(2):487-99. Epub 2013 Sep 12.

College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, Jiangsu Province 210095, China; Shandong Binzhou Animal Science and Veterinary Medicine Institute, Binzhou 256600, China.

View Article
November 2013
10 Reads
8 PubMed Central Citations(source)
3.94 Impact Factor

Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo.

Arch Virol 2013 Feb 7;158(2):349-58. Epub 2012 Oct 7.

College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, Jiangsu Province, China.

View Article
February 2013
2 Reads
17 PubMed Central Citations(source)
2.28 Impact Factor

Inhibition of Japanese encephalitis virus infection in vitro and in vivo by pokeweed antiviral protein.

Virus Res 2013 Jan 8;171(1):89-96. Epub 2012 Nov 8.

College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, Jiangsu Province, 210095, PR China.

View Article
January 2013
1 Read
1 PubMed Central Citation(source)
2.32 Impact Factor

Porcine reproductive and respiratory syndrome virus induces autophagy to promote virus replication.

Autophagy 2012 Oct 28;8(10):1434-47. Epub 2012 Jun 28.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.

View Article
October 2012
2 Reads
20 PubMed Central Citations(source)
11.75 Impact Factor

A bursal pentapeptide (BPP-I), a novel bursal-derived peptide, exhibits antiproliferation of tumor cell and immunomodulator activity.

Amino Acids 2012 Jun 13;42(6):2215-22. Epub 2011 Jul 13.

Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, 210095, People's Republic of China.

View Article
June 2012
6 Reads
2 PubMed Central Citations(source)
3.29 Impact Factor

Deletion of the single putative N-glycosylation site of the porcine circovirus type 2 Cap protein enhances specific immune responses by DNA immunisation in mice.

Vet J 2012 Jun 19;192(3):385-9. Epub 2011 Oct 19.

Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agriculture University, Nanjing 210095, Jiangsu, China.

View Article
June 2012
6 Reads
1.75 Impact Factor

Inhibition of Japanese encephalitis virus entry into the cells by the envelope glycoprotein domain III (EDIII) and the loop3 peptide derived from EDIII.

Antiviral Res 2012 May 10;94(2):179-83. Epub 2012 Mar 10.

College of Veterinary Medicine, Nanjing Agricultural University, Weigang, Nanjing, Jiangsu Province, China.

View Article
May 2012
3 Reads
3 PubMed Central Citations(source)
3.94 Impact Factor

Identification and characterization of novel immunomodulatory bursal-derived pentapeptide-II (BPP-II).

J Biol Chem 2012 Feb 19;287(6):3798-807. Epub 2011 Dec 19.

Division of Key Laboratory of Animal Disease Diagnosis and Immunology, Department of Agriculture of China, Nanjing Agricultural University, Nanjing 210095, China.

View Article
February 2012
1 Read
4.57 Impact Factor

The design and recombinant protein expression of a consensus porcine interferon: CoPoIFN-α.

Cytokine 2012 Jan 15;57(1):37-45. Epub 2011 Nov 15.

Key Laboratory of Animal Disease Diagnostic & Immunology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.

View Article
January 2012
5 Reads
2 PubMed Central Citations(source)
2.66 Impact Factor

Design and evaluation of a multi-epitope peptide against Japanese encephalitis virus infection in BALB/c mice.

Biochem Biophys Res Commun 2010 Jun 10;396(4):787-92. Epub 2010 May 10.

Key Laboratory of Animal Disease Diagnosis and Immunology, Ministry of Agriculture at Nanjing Agricultural University, Nanjing 210095, China.

View Article
June 2010
1 Read
5 PubMed Central Citations(source)
2.30 Impact Factor
Top co-authors
Xiang Mao
Xiang Mao

College of Veterinary Medicine

10
Chen Li
Chen Li

Catalytic Hydrogenation Research Center

8
Li Huang
Li Huang

Guangxi Key Laboratory of Veterinary Biotechnology

8
Qiyan Xiong
Qiyan Xiong

China Pharmaceutical University

4
Ming-Xia Sun
Ming-Xia Sun

College of Veterinary Medicine

4
Bin Zhou
Bin Zhou

The State Key Laboratory of Cell Biology

4
Maojun Liu
Maojun Liu

Institute of Veterinary Medicine

4
Zhixin Feng
Zhixin Feng

College of Veterinary Medicine

4
Guoqing Shao
Guoqing Shao

Huazhong Agricultural University

4
Ming-Xia Sun
Ming-Xia Sun

College of Veterinary Medicine

3