Publications by authors named "Hassan Jalalizadeh"

10 Publications

  • Page 1 of 1

A Stability-Indicating HPLC Method for the Determination of Memantine Hydrochloride in Dosage Forms through Derivatization with 1-Fluoro-2,4-dinitrobenzene.

Sci Pharm 2014 Apr-Jun;82(2):265-79. Epub 2013 Dec 9.

Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Memantine is chemically a tricyclic amine and is used for Parkinson's disease and movement disorders. Although several HPLC methods with different derivatization reagents have been developed for the determination of memantine in biological fluids, there are some complications which limit the use of these methods in routine analysis of memantine in in vitro tests. We established a simple, sensitive, precise, and accurate HPLC method for the quantification of memantine in dosage forms. Pre-column derivatization of memantine was performed with 1-fluoro-2,4-dinitrobenzene and the reaction product was separated on a Nova-Pak C18 column. A mixture of acetonitrile and sodium dihydrogenphosphate (pH 2.5; 0.05 M) (70: 30, v/v) was used as the mobile phase. UV detection was performed at 360 nm. Forced degradation studies were performed on a powdered tablet sample of memantine hydro-chloride using acidic (0.1 M hydrochloric acid), basic (0.1 M sodium hydroxide), oxidative (10% hydrogen peroxide), thermal (105°C), photolytic, and humidity conditions. Good linearity (r(2)=0.999) was obtained over the range of 1-12 μg mL(-1) of memantine hydrochloride with acceptable within-day and between-day precision values in the range of 0.05-0.95%. The proposed method was used for the assay determination and dissolution rate study of memantine dosage forms with excellent specificity.
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http://dx.doi.org/10.3797/scipharm.1310-09DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065122PMC
June 2014

Development and validation of a simple and rapid HPLC method for determination of pioglitazone in human plasma and its application to a pharmacokinetic study.

J Chromatogr Sci 2008 Oct;46(9):809-12

Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Medical Sciences / University of Tehran, Tehran (14155-6451), Iran.

In this study, a new, simple, and reproducible high-performance liquid chromatographic method was developed for the determination of pioglitazone in human plasma. After liquid-liquid extraction with diethylether, samples were quantitated on a Nova-Pak C8 column using a mixture of acetonitrile-140mM K2HPO4 (40:60, v/v, pH = 4.45) as mobile phase with UV detection at 269 nm. The flow rate was set at 1.4 mL/min. Ethylparaben was used as internal standard and the total run time of analysis was approximately 7 min. The method was linear over the range of 25-1500 ng/mL of pioglitazone in plasma (r2 > 0.999). The within- and between-day precision values were in the range of 2.4-6.8%. The limit of quantitation of the method was 25 ng/mL. The method was successfully used to study the pharmacokinetics of pioglitazone in healthy volunteers.
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http://dx.doi.org/10.1093/chromsci/46.9.809DOI Listing
October 2008

A time course analysis of systemic administration of aqueous licorice extract on spatial memory retention in rats.

Planta Med 2008 Apr 10;74(5):485-90. Epub 2008 Apr 10.

Department of Toxicology-Pharmacology and Center of Excellence of Toxicology, Pharmaceutical Sciences and Medicinal Plants Research Centers, Faculty of Pharmacy, Tehran University of Medicinal Sciences, Tehran, Iran.

In the present study, the time course of the effects of Glycyrrhiza glabra L. (Leguminosae) aqueous extract (GE), administered systemically to rats, on the spatial memory retention in the Morris water maze was investigated. The dose of glycyrrhizin (GL), i. e., 0.5, 2.5 and 5 mg/mL in daily water intake of GE was administered to three groups of rats. The first, second and third groups received GE for 1, 2 and 4 weeks, respectively (each group included 3 subgroups). Three additional control groups of animals received only tap water during the same periods of time. After terminating the treatments, all animals were trained for four days; each day included one block and each block contained four trials. Test trials were conducted 48 h after the completion of the training period. Nicotine (1 microg/side) was infused into the CA1 region of the hippocampus as a positive drug control. GE treatment decreased both escape latency and traveled distance, but not swimming speed, compared with control, suggesting significant spatial memory retention enhancement by GE. Statistical analysis did not show any significant difference between GE-treated animals and the nicotine group in escape latency and traveled distance. At the end of the testing trials plasma samples were collected and the concentrations of glycyrrhetinic acid (GA) as a major metabolite of GL were measured in the different groups of treated rats. The maximum concentration was observed after four weeks of GE administration at 5 mg/mL of GL. These results showed that the enhancement effect of GE on spatial memory retention does not correlate with GA blood levels.
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http://dx.doi.org/10.1055/s-2008-1074494DOI Listing
April 2008

Optimization of an HPLC method for determination of gabapentin in dosage forms through derivatization with 1-fluoro-2,4-dinitrobenzene.

Chem Pharm Bull (Tokyo) 2007 Oct;55(10):1427-30

Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Medical Sciences/University of Tehran, Iran.

A rapid, sensitive and accurate high performance liquid chromatography with UV detection method was developed and validated for the quantification of gabapentin in dosage forms. Gabapentin was quantified after pre-column derivatization with 1-fluoro-2,4-dinitrobenzene. Amlodipine was used as an internal standard. The chromatographic separation was carried out on a Nova-Pak C(18) column using a mixture of acetonitrile-sodium dihydrogenphosphate (pH 2.5; 0.05 M) (70:30, v/v) as mobile phase with UV detection at 360 nm. The method was linear over the range of 10-500 microg/ml of gabapentin (r(2)>0.999). The within-day and between-day precision values were in the range of 0.86-1.11%. The method was successfully used for quantitative determination and dissolution rate study of Neurontin capsules.
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http://dx.doi.org/10.1248/cpb.55.1427DOI Listing
October 2007

Validated HPLC method for the determination of gabapentin in human plasma using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene and its application to a pharmacokinetic study.

J Chromatogr B Analyt Technol Biomed Life Sci 2007 Jul 5;854(1-2):43-7. Epub 2007 Apr 5.

Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 14155-6451, Iran.

A rapid, sensitive and accurate high-performance liquid chromatographic method with UV detection was developed and validated for the quantification of gabapentin in human plasma. Gabapentin was quantified using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene following protein precipitation of plasma with acetonitrile. Amlodipine was used as internal standard. The chromatographic separation was carried out on a Nova-Pak C(18) column using a mixture of 50 mM NaH(2)PO(4) (pH=2.5)-acetonitrile (30:70, v/v) as mobile phase with UV detection at 360 nm. The flow rate was set at 1.5 ml/min. The method was linear over the range of 0.05-5 microg/ml of gabapentin in plasma (r(2)>0.999). The within-day and between-day precision values were in the range of 2-5%. The limit of quantification of the method was 0.05 microg/ml. The method was successfully used to study the pharmacokinetics of gabapentin in healthy volunteers.
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http://dx.doi.org/10.1016/j.jchromb.2007.03.039DOI Listing
July 2007

HPLC analysis of orlistat and its application to drug quality control studies.

Chem Pharm Bull (Tokyo) 2007 Feb;55(2):251-4

Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.

In this study, a high performance liquid chromatography method with UV detection was developed for determination of orlistat. The chromatographic system consisted of a Nova-Pack C18 column, an isocratic mobile phase of phosphoric acid 0.1%-acetonitrile (10 : 90, v/v) and UV detection at 205 nm. Orlistat was eluted at about 6 min with no interfering peak from excipients used for preparation of dosage form. The method was linear over the range of 10-160 microg/ml orlistat (r2 > 0.9999). The within-day and between-day precision values were also in the range of 0.10-0.59%. The appropriate dissolution conditions were also determined and applied to evaluate the dissolution profile of orlistat capsules. Optimal conditions were 1000 ml of 3% SLS in water as dissolution medium and paddle at 100 rotation per minute. The proposed method was applied successfully to the determination of orlistat content in capsules and in vitro dissolution studies.
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http://dx.doi.org/10.1248/cpb.55.251DOI Listing
February 2007

Validated HPLC method for determination of carboxylic acid metabolite of clopidogrel in human plasma and its application to a pharmacokinetic study.

Biomed Chromatogr 2006 Dec;20(12):1309-14

Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), Iran.

A new, simple, and reproducible method for determination of carboxylic acid metabolite of clopidogrel in human plasma has been developed. After liquid-liquid extraction in acidic medium with chloroform, samples were quantified on a Nova-pak C(8), 5 microm column using a mixture of 30 mM K(2)HPO(4)-THF-acetonitrile (pH = 3, 79:2:19, v/v/v) as mobile phase with UV detection at 220 nm. The flow rate was set at 0.9 mL/min. Ticlopidine was used as internal standard and the total run time of analysis was about 12 min. The method was linear over the range of 0.2-10 microg/mL of clopidogrel metabolite in plasma (r(2) > 0.999). The within-day and between-day precision values were in the range 1.0-4.8%. The limit of quantification of the method was 0.2 microg/mL. The method was successfully used to study the pharmacokinetics of clopidogrel in healthy volunteers.
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http://dx.doi.org/10.1002/bmc.697DOI Listing
December 2006

Synthesis and analgesic activity of 2-phenoxybenzoic acid and N-phenylanthranilic acid hydrazides.

Biol Pharm Bull 2006 Jun;29(6):1180-5

Department of Medicinal Chemistry, Faculty of Pharmacy, Islamic Azad University, Tehran, Iran.

A series of 2-phenoxybenzoic acid and N-phenylanthranilic acid hydrazides were synthesized and evaluated for their analgesic activities. Several compounds were significantly more potent than mefenamic acid and diclofenac sodium in abdominal constriction and formalin tests.
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http://dx.doi.org/10.1248/bpb.29.1180DOI Listing
June 2006

Simultaneous determination of cyproterone acetate and ethinylestradiol in tablets by derivative spectrophotometry.

Chem Pharm Bull (Tokyo) 2005 Aug;53(8):949-51

Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), Iran.

Derivative spectrophotometry offers a useful approach for the analysis of drugs in multi-component mixtures. In this study a third-derivative spectrophotometric method was used for simultaneous determination of cyproterone acetate and ethinylestradiol using the zero-crossing technique. The measurements were carried out at wavelengths of 316 and 226 nm for cyproterone acetate and ethinylestradiol respectively. The method was found to be linear (r2>0.999) in the range of 0.5-6 mg/100 ml for cyproterone acetate in the presence of 35 microg/100 ml ethinylestsradiol at 316 nm. The same linear correlation (r2>0.999) was obtained in the range of 10-80 microg/100 ml of ethinylestradiol in the presence of 2 mg/100 ml of cyproterone acetate at 226 nm. The limit of determination was 0.5 mg/100 ml and 10 microg/100 ml for cyproterone acetate and ethinylestradiol respectively. The method was successfully applied for simultaneous determination of cyproterone acetate and ethinylestradiol in pharmaceutical preparations without any interferences from excipients.
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http://dx.doi.org/10.1248/cpb.53.949DOI Listing
August 2005

Determination of celecoxib in human plasma by high-performance liquid chromatography.

J Pharm Biomed Anal 2004 May;35(3):665-70

Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

A high performance liquid chromatographic method for the quantitation of celecoxib (CEL) in human plasma is presented. The method is based on liquid-liquid extraction with chloroform and reversed-phase chromatography using a Nucleosil CN column (250 mm x 4.6 mm i.d., 5 microm particle size) and UV spectrophotometer detection at 260 nm. The mobile phase consists of acetonitrile:water (60:40 (v/v)). Flutamide was used as internal standard (IS). The assay was linear in the concentration range of 10-1000 ng/ml when 0.5 ml aliquots of plasma were extracted. Within-day and between-day precision expressed by relative standard deviation is less than 4% and inaccuracy does not exceed 3%. The assay was used to analyze samples collected during human clinical studies.
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http://dx.doi.org/10.1016/j.jpba.2004.02.005DOI Listing
May 2004