Publications by authors named "Haroldo Vieira de Moraes Junior"

8 Publications

  • Page 1 of 1

Reduced occurrence of programmed cell death and gliosis in the retinas of juvenile rabbits after shortterm treatment with intravitreous bevacizumab.

Clinics (Sao Paulo) 2012 ;67(1):61-7

Federal University of Rio de Janeiro, Institute of Biophysics, Brazil.

Objective: Bevacizumab has been widely used as a vascular endothelial growth factor antagonist in the treatment of retinal vasoproliferative disorders in adults and, more recently, in infants with retinopathy of prematurity. Recently, it has been proposed that vascular endothelial growth factor acts as a protective factor for neurons and glial cells, particularly in developing nervous tissue. The purpose of this study was to investigate the effects of bevacizumab on the developing retinas of juvenile rabbits.

Methods: Juvenile rabbits received bevacizumab intravitreously in one eye; the other eye acted as an untreated control. Slit-lamp and fundoscopic examinations were performed both prior to and seven days after treatment. At the same time, retina samples were analyzed using immunohistochemistry to detect autophagy and apoptosis as well as proliferation and glial reactivity. Morphometric analyses were performed, and the data were analyzed using the Mann-Whitney U test.

Results: No clinical abnormalities were observed in either treated or untreated eyes. However, immunohistochemical analyses revealed a reduction in the occurrence of programmed cell death and increases in both proliferation and reactivity in the bevacizumab-treated group compared with the untreated group.

Conclusions: Bevacizumab appears to alter programmed cell death patterns and promote gliosis in the developing retinas of rabbits; therefore, it should be used with caution in developing eyes.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248603PMC
http://dx.doi.org/10.6061/clinics/2012(01)10DOI Listing
September 2012

[Ocular and electroretinographic changes in sickle cell disease].

Arq Bras Oftalmol 2011 May-Jun;74(3):190-4

Departamento de Oftalmologia do Hospital Universitário Clementino Fraga Filho - Universidade Federal do Rio de Janeiro (RJ) - Brazil.

Purpose: The primary objective of this case series was to get electroretinographic data in subjects with sickle cell disease. The secondary purpose was to identify ocular changes, their frequency and stage of evolution.

Methods: 90 patients aged between 8 and 75 years were evaluated. They were subjected to complete ophthalmological examination, indirect ophthalmoscopy, retinography, angiography and electroretinography. Electroretinograms with amplitude of wave b less than 400 mW were considered subnormal. Prevalence study was conducted using the Chi-square test, P<0.001.

Results: The electroretinogram was subnormal in 27 (30%) of 90 patients, but of these, only 4 (4.4%) had proliferative retinopathy. The sign of comma in 95% of individuals, the retinal vascular tortuosity in 36.6% and black sunburst in 24.4% were the most frequent changes.

Conclusion: No correlation between sickle cell disease and electroretinographics changes, as described in other studies, were noted. On the other hand, the ocular findings corroborate the previous researches.
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http://dx.doi.org/10.1590/s0004-27492011000300009DOI Listing
March 2012

[Subclinical diabetic macular edema and mild non-proliferative diabetic retinopathy: data correlation with the retinal thickness analyzer (RTA)].

Arq Bras Oftalmol 2009 Jul-Aug;72(4):503-8

Departamento de Retina e Vítreo da Universidade Federal de Goiás, UFG, Goiânia, Brazil.

Purpose: To measure, using the retinal thickness analyzer, the mean foveal thickness and the mean perifoveal thickness values on patients suffering from mild non-proliferative diabetic retinopathy without clinically detectable macular edema (classified by the ETDRS study), and compare them with healthy individuals.

Methods: Observational analytical retrospective case-controlled study, on a sample of 79 eyes (39 affected and 40 control). The sample was selected from by exams previously taken at Centro Brasileiro de Cirurgia de Olhos (CBCO) (from 1999 to 2003) stored in the retinal thickness analyzer. The control group was selected according to specific criteria. The affected group was formed according to the research inclusive and exclusive criteria. The results were submitted to statistical tests for reliability.

Results: In the control group the values obtained for mean foveal thickness were around (mean=147.4 micra+/-15.4 micra standard deviation) and for mean perifoveal thickness were around (mean=193.8 micra+/-24.6 micra standard deviation). Patients with mild non-proliferative diabetic retinopathy presented foveal thickness around (mean=198.9 micra+/-48.3 micra standard deviation) and perifoveal thickness around (mean=194.2 micra+/-26.4 micra standard deviation).

Conclusion: An increase of mean foveal thickness on diabetic patients who have not shown clinical signs of macular edema was noted, which was proved by statistical tests. Such results are similar to the values found in medical literature.
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http://dx.doi.org/10.1590/s0004-27492009000400013DOI Listing
March 2010

[Correlation between central corneal thickness and axial length in patients with glaucoma and normal eyes].

Arq Bras Oftalmol 2009 Jul-Aug;72(4):497-502

Departamento de Oftalmologia Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

Purpose: To evaluate and to correlate the central corneal thickness with the ocular axial length in patients with primary open-angle glaucoma, primary angle-closure glaucoma and individuals with normal eyes.

Methods: The sample was composed of 94 patients' eyes, divided into three groups constituted of 33 eyes of 33 primary open-angle glaucoma patients, 30 eyes of 30 primary angle-closure glaucoma patients and 31 normal eyes of 31 individuals. The central corneal thickness and the axial length were measured by ultrasonic pachymeter and biometry by A-scan ultrasound, respectively.

Results: The results showed a mean of 535.1 mm central corneal thickness in primary angle-closure glaucoma group, 520.6 mm in primary open-angle glaucoma group and 519.2 mm in normal eyes (p=0.18). The ocular axial length on primary angle-closure glaucoma group was 22.16 mm and on primary open-angle glaucoma and normal eyes group was 22.68 mm and 22.64 mm, respectively (p=0.13). There was no significant correlation between corneal thickness and axial length in the primary angle-closure glaucoma group (r=-0.085; p=0.65), the open-angle glaucoma group (r=-0.070; p=0.69) and the normal eyes group (r=-0.120; p=0.52).

Conclusions: The results of this assay suggest that there is no correlation between the central corneal thickness and the axial length in patients with glaucoma and individuals with normal eyes.
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http://dx.doi.org/10.1590/s0004-27492009000400012DOI Listing
March 2010

[Comparative study between diurnal intraocular pressure curve and the association of ambulatory intraocular pressure curve with the water-drinking test in open angle glaucoma, normal tension glaucoma and normal eyes].

Arq Bras Oftalmol 2007 May-Jun;70(3):471-9

Universidade Gama Filho, Brasil.

Purpose: To evaluate and correlate the peaks and the fluctuation of intraocular pressure seen in the association of the ambulatory intraocular pressure curve with the water-drinking test versus the peaks and the fluctuation seen in the daily intraocular pressure curve.

Methods: The sample was as follows: 77 eyes belonging to 77 patients who were divided into three groups composed of 31 eyes belonging to 31 patients with primary open-angle glaucoma, 26 eyes belonging to 26 patients with normal tension glaucoma and 20 normal eyes belonging to 20 individuals.

Results: A significant correlation could be observed between the pressure peaks collected from the daily intraocular pressure curve and the pressure peaks seen in the ambulatory intraocular pressure curve, on the water-drinking test, as well as in the association of the ambulatory intraocular pressure curve with the water-drinking test. The procedure which showed the highest rate of correlation between the pressure peaks and the peaks of the daily intraocular pressure curve was the ambulatory intraocular pressure curve (r2= 0.81). However, no statistically significant difference was found between the pressure peaks and the correlation coefficients observed in the other methods. The correlation between the fluctuation of intraocular pressure obtained in the association of the ambulatory intraocular pressure curve with the water-drinking test and the fluctuation of the pressure seen in the daily intraocular pressure curve showed a slight association (r2= 0.21).

Conclusions: The association between ambulatory intraocular pressure curve and water-drinking test was not efficient to estimate peaks and the fluctuation of daily intraocular pressure curve. The ambulatory intraocular pressure curve and the water-drinking test must be analyzed separately. When predicting the peak and the fluctuation of the daily intraocular pressure curve, the ambulatory intraocular pressure curve was the most efficient procedure.
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http://dx.doi.org/10.1590/s0004-27492007000300015DOI Listing
February 2008

[Electroretinographic study of chromatic vision].

Arq Bras Oftalmol 2006 Nov-Dec;69(6):857-63

Universidade Federal do Rio de Janeiro, Rua Euzébio Naylor 155, Rio de Janeiro, RJ CEP 22793-770, Brazil.

Purpose: To describe the electroretinogram of the South-American opossum (Didelphis aurita) obtained by chromatic stimulus of specific wavelengths. The electroretinogram records voltage variations of retinal cells triggered by light stimulation. The electroretinogram represents the combination of electric activity of many different cells and varies according to retinal physiology and examination methods.

Methods: We recorded the electroretinogram of six animals in dark adaptation using chromatic Kodak Wratten filters, and recorded the spectral sensitivity to specific wavelengths in the spectrum of blue, green, yellow, orange and red light bands.

Results: The most consistent electrorretinographic results were obtained when the animals were stimulated by selective spectral bands instead of white light. These results are consistent with the absorbance curve of the opsins described in marsupial photoreceptors. Previous studies using microspectrophotometry of opsins and retinal immunohistochemistry suggested marsupial trichromacy. This morphologic knowledge has not before been physiologically demonstrated by electroretinographic methods.

Conclusion: The South-American opossum has proven to be an interesting experimental animal for comparative visual physiology studies among other mammals, especially studies on phylogenetic of chromatic vision. The opossum represents a retinal model that superimposes both the photopic and scotopic systems; and the Didelphis genus shows few changes when compared to the fossils of the Pleocene period. Therefore the marsupial's visual system retrieves characteristics from ancient mammal evolution to the retinal patterns found in modern mammals.
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http://dx.doi.org/10.1590/s0004-27492006000600014DOI Listing
December 2007

[Ophthalmological alterations in outpatients with systemic lupus erythematosus].

Arq Bras Oftalmol 2006 Mar-Apr;69(2):233-7. Epub 2006 May 8.

Departamento de Oftalmologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

Purpose: To evaluate the ophthalmological alterations in patients with systemic lupus erythematosus (SLE).

Methods: A descriptive cross-sectional study was conducted to analyze 70 patients with diagnosis of SLE based on the American College of Rheumatology criteria. Data regarding sex, age, ethnicity, duration of disease, disease activity, use of corticosteroids and antimalarial drugs, and presence of ophthalmological symptoms were recorded and a full ophthalmic examination was carried out.

Results: Sixty-two patients (88.6%) were females, 43 patients (61.4%) were white. Mean age was 43.9 years, 57 patients (81.4%) presented no sign of active disease and 46 patients (65.7%) had no ophthalmological complaints. Dry eye syndrome was diagnosed in 22 patients (31.4%), bilateral subcapsular cataract in 15 patients (21.1%) and glaucoma in 2 patients (2.9%). Fundoscopic evaluation disclosed 8 patients (11.4%) with drusen, 7 (10.0%) with RPE atrophy, 2 patients (2.9%) presented pathological disc excavation, 2 (2.9%) vascular tortuosity and arteriovenous crossing changes, 1 patient (1.4%) had macular chorioretinitis scar and 1 patient (1.4%) had branch retinal vein occlusion.

Conclusion: SLE is a disease that can present with ophthalmic involvement, usually benign in outpatients. Dry eye and cataract are the commonest ocular findings. The former seems to be more related to SLE itself while the latter probably relates to the chronic use of corticosteroids for the disease treatment. Antimalarial drugs do not frequently cause ophthalmic lesions, although further studies must be conducted in order to assess their impact on patients' long-term visual quality. In the patients with the disease under control, ocular damage is more related to systemic treatment what enhances the need for ophthalmological examination as a routine even in these patients.
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http://dx.doi.org/10.1590/s0004-27492006000200018DOI Listing
October 2006