Publications by authors named "Haoxiang Chen"

15 Publications

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Percutaneous spinal endoscopy with unilateral interlaminar approach to perform bilateral decompression for central lumbar spinal stenosis: radiographic and clinical assessment.

BMC Musculoskelet Disord 2021 Mar 1;22(1):236. Epub 2021 Mar 1.

Department of Spine Surgery, The First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.

Background: Recently, a percutaneous spinal endoscopy unilateral posterior interlaminar approach to perform bilateral decompression has been proposed for use in treatment of lumbar spinal stenosis, As a development and supplement to traditional surgery, its advantages regarding therapeutic effects and prognosis, such as minor soft tissue damage, little intraoperative blood loss, and a quick return to daily life. However, there are few analyses of this surgery with a follow-up of more than 1 year,we conducted this study in order to quantitatively investigate radiographic and clinical efficacies of this surgery for central lumbar spinal stenosis.

Materials And Methods: Forty-six patients with central lumbar spinal stenosis were enrolled from January 2017 to July 2018. The visual analog scale (VAS) for back pain and leg pain, Oswestry disability index (ODI), modified MacNab criteria were used to evaluate clinical efficiency at preoperative and postoperative time points. The intervertebral height index (IHI), cross-sectional area of the spinal canal (CSAC), calibrated disc signal (CDS) and spinal stability were examined to assess radiographic decompression efficiency via magnetic resonance imaging and X-ray at preoperative and postoperative time points.

Results: The VAS score for lower back pain and leg pain improved from 7.50 ± 0.78 to 1.70 ± 0.66 and from 7.30 ± 0.79 to 1.74 ± 0.68, respectively, and the ODI improved from 72.35 ± 8.15 to 16.15 ± 4.51. In terms of modified MacNab criteria, 91.3% of the patients achieved good or excellent outcomes. Furthermore, significant changes after surgery were observed for the percentage of CSAC, increasing from 125.3 ± 53.9 to 201.4 ± 78 mm; however, no significant differences were observed for the remaining measurement indicators.

Conclusions: The clinical and radiographic efficacies of this surgery for central lumbar spinal stenosis were good in short-term follow-up, and this surgery did not cause meaningful changes in IHI, CDS, and spine stability in short-term follow-up. The effect of long-term follow-up needs further investigation.
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http://dx.doi.org/10.1186/s12891-021-04100-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923329PMC
March 2021

Gut-Liver Immune Response and Gut Microbiota Profiling Reveal the Pathogenic Mechanisms of in Pearl Gentian Grouper ( × ).

Front Immunol 2020 26;11:607754. Epub 2020 Nov 26.

Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.

causes vibriosis in nearly 70% of grouper ( sp.), seriously limiting grouper culture. As well as directly inhibiting pathogens, the gut microbiota plays critical roles in immune homeostasis and provides essential health benefits to its host. However, there is still little information about the variations in the immune response to infection and the gut microbiota of grouper. To understand the virulence mechanism of in the pearl gentian grouper, we investigated the variations in the pathological changes, immune responses, and gut bacterial communities of pearl gentian grouper after exposure to differently virulent strains. Obvious histopathological changes were detected in heart, kidney, and liver. In particular, nodules appeared and huge numbers of cells colonized the liver at 12 h postinfection (hpi) with highly virulent Although no was detected in the gut, the infection simultaneously induced a gut-liver immune response. In particular, the expression of 8 genes associated with cellular immune processes, including genes encoding inflammatory cytokines and receptors, and pattern recognition proteins, was markedly induced by infection, especially with the highly virulent strain. infection also induced significant changes in gut bacterial community, in which and increased but , , , and decreased in the group infected with the highly virulent strain, with accounting for 82.01% dissimilarity. Correspondingly, four bacterial functions related to bacterial pathogenesis were increased by infection with highly virulent , whereas functions involving metabolism and genetic information processing were reduced. These findings indicate that colonizes the liver and induces a gut-liver immune response that substantially disrupts the composition of and interspecies interactions in the bacterial community in fish gut, thereby altering the gut-microbiota-mediated functions and inducing fish death.
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http://dx.doi.org/10.3389/fimmu.2020.607754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727329PMC
November 2020

3D printed tumor tissue model of colorectal cancer.

Theranostics 2020 26;10(26):12127-12143. Epub 2020 Oct 26.

College of Biology, Hunan University, Changsha, Hunan, 410082, China.

The tumor microenvironment (TME) determines tumor progression and affects clinical therapy. Its basic components include cancer-associated fibroblasts (CAFs) and tumor-associated endothelial cells (TECs), both of which constitute the tumor matrix and microvascular network. The ability to simulate interactions between cells and extracellular matrix in a TME can assist the elucidation of cancer growth and evaluate the efficiency of therapies. In the present study, an 3D model of tumor tissue that mimicked cell physiological function was developed using tumor-associated stromal cells. Colorectal cancer cells, CAFs, and TECs were co-cultured on 3D-printed scaffolds so as to constitute an extracellular matrix (ECM) that allowed cell processes such as adhesion, stemness, proliferation, and vascularization to take place. Normal stromal cells were activated and reprogrammed into tumor-related stromal cells to construct a TME of tumor tissues. The activated stromal cells overexpressed a variety of tumor-related markers and remodeled the ECM. Furthermore, the metabolic signals and malignant transformation of the 3D tumor tissue was substantially similar to that observed in tumors . The 3D tumor tissue exhibited physiological activity with high drug resistance. The model is suitable for research studies of tumor biology and the development of personalized treatments for cancer.
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http://dx.doi.org/10.7150/thno.52450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667682PMC
October 2020

3D printed intelligent scaffold prevents recurrence and distal metastasis of breast cancer.

Theranostics 2020 29;10(23):10652-10664. Epub 2020 Aug 29.

College of Biology, Hunan University, Changsha, Hunan, 410082, China.

Tumors are commonly treated by resection, which usually leads to massive hemorrhage and tumor cell residues, thereby increasing the risk of local recurrence and distant metastasis. Herein, an intelligent 3D-printed poly(lactic-co-glycolic acid), gelatin, and chitosan scaffold loaded with anti-cancer drugs was prepared that showed hemostatic function and good pH sensitivity. Following implantation in wounds, the scaffolds absorbed hemorrhage and cell residues after surgery, and promoted wound healing. In an environment, the scaffold responded to the slightly acidic environment of the tumor to undergo sustained drug release to significantly inhibit the recurrence and growth of the tumor, and reduced drug toxicity, all without causing damage to healthy tissues and with good biocompatibility. The multifunctional intelligent scaffold represents an excellent treatment modality for breast cancer following resection, and provides great potential for efficient cancer therapy.
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http://dx.doi.org/10.7150/thno.47933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482818PMC
August 2020

Prevalence, virulence genes, and antimicrobial resistance of Vibrio species isolated from diseased marine fish in South China.

Sci Rep 2020 08 31;10(1):14329. Epub 2020 Aug 31.

Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.

Here, 70 potential Vibrio pathogens belonging to nine species, dominated by Vibrio harveyi, were isolated and identified from diseased aquacultured marine fish in South China. Subsequently, the prevalence of 11 virulence genes and the resistance to 15 antibiotics in these strains were determined. Most strains possessed atypical virulence genes in addition to typical virulence genes. Notably, hflk and chiA originating from V. harveyi, and flaC associated with V. anguillarum were detected in more than 40% of atypical host strains. Multidrug resistance was widespread: 64.29% strains were resistant to more than three antibiotics, and the multi-antibiotic resistance index ranged from 0.00 to 0.60. The proportions of strains resistant to the antibiotics vancomycin, amoxicillin, midecamycin, and furazolidone all exceeded 50%; nevertheless, all strains were sensitive to florfenicol, norfloxacin, and ciprofloxacin. Furthermore, both virulence genes and antibiotic resistance were more prevalent in Hainan than in Guangdong, owing to the warmer climate and longer annual farming time in Hainan. These results therefore suggest that warming temperatures and overuse of antibiotics are probably enhancing antibiotic resistance and bacterial infection. This study reveals that pathogenic Vibrio spp. with multi-antibiotic resistance are highly prevalent among marine fish in South China and thus warrant further attention. The results will provide helpful guidance for ecological regulation and local antibiotic use in the control of marine fish farming' Vibrio diseases in South China, facilitating the implementation of national green and healthful aquaculture.
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http://dx.doi.org/10.1038/s41598-020-71288-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459350PMC
August 2020

Ultra-compact reflective mode converter based on a silicon subwavelength structure.

Appl Opt 2020 Mar;59(9):2754-2758

Mode converters play an essential role in mode-division multiplexing systems. A reflective mode converter (RMC), which is utilized to accomplish the mode conversion in the contra-propagation process, can fold the optical path and realize the mode exchange in an optical network. In this paper, we propose and experimentally demonstrate an RMC based on a silicon subwavelength structure. It can convert the input fundamental mode (${\text{TE}_0}$TE) into the first-order mode (${\text{TE}_1}$TE) in a ${2.0}\;\unicode{x00B5} \text{m} \times {2.0}\;\unicode{x00B5} \text{m}$2.0µm×2.0µm footprint. The simulated insertion loss and cross talk are lower than 0.6 dB and $ - {20.3}\;\text{dB}$-20.3dB in 1525-1565 nm. Experimental results verify the functionality of the device. The measured insertion loss and cross talk are lower than 2.2 dB and $ - {16.2}\;\text{dB}$-16.2dB. To further prove the generality of the methodology, we design another two RMCs realizing the mode conversion functions of ${\text{TE}_0}$TE to ${\text{TE}_2}$TE and ${\text{TE}_0}$TE to ${\text{TE}_3}$TE modes. The simulated insertion losses are lower than 1.1 dB and 1.8 dB.
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http://dx.doi.org/10.1364/AO.388787DOI Listing
March 2020

Rapid environmental change shapes pond water microbial community structure and function, affecting mud crab (Scylla paramamosain) survivability.

Appl Microbiol Biotechnol 2020 Mar 8;104(5):2229-2241. Epub 2020 Jan 8.

Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.

The aquatic microbial community is sensitive to environmental change; however, the impacts of those changes combined with disease outbreaks affecting S. paramamosain are unknown. Thus, from March to October, we explored the interaction between aquacultural environmental conditions and microbial composition and function in open-air aquaculture ponds containing S. paramamosain in Southern China. The microbial community structure was significantly positively correlated with microbial community function. The environment variables such as temperature and salinity during May and June changed more quickly compared with other periods, resulting changes in the structure and function of the microbial community affected S. paramamosain survivability, with higher crab mortality observed from May to June compared with other periods. These included changes in the relative abundance of Microtrichales, Synechococcales, Rhodobacterales, Chitinophagales, and SAR11_clade, and corresponding functions associated with glycolysis and/or gluconeogenesis, porphyrin and chlorophyll metabolism, photosynthetic proteins, and transcription factors. These changes could impact S. paramamosain mortality and be used to evaluate the health status of the ponds. Though the environment variables during July~October changed slowly comparing to May and June, the ponds microflora changed which benefit S. paramamosain survivability with correspondingly low S. paramamosain mortality. Therefore, rapid environmental change alters the structure and function of the aquatic microflora, increasing S. paramamosain mortality.
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http://dx.doi.org/10.1007/s00253-019-10328-wDOI Listing
March 2020

E-jet 3D printed drug delivery implants to inhibit growth and metastasis of orthotopic breast cancer.

Biomaterials 2020 02 13;230:119618. Epub 2019 Nov 13.

College of Biology, Hunan University, Changsha, Hunan, 410082, China; Shenzhen Institute, Hunan University, Shenzhen, Guangdong, 410082, China. Electronic address:

Drug-loaded implants have attracted considerable attention in cancer treatment due to their precise delivery of drugs into cancer tissues. Contrary to injected drug delivery, the application of drug-loaded implants remains underutilized given the requirement for a surgical operation. Nevertheless, drug-loaded implants have several advantages, including a reduction in frequency of drug administration, minimal systemic toxicity, and increased delivery efficacy. Herein, we developed a new, precise, drug delivery device for orthotopic breast cancer therapy able to suppress breast tumor growth and reduce pulmonary metastasis using combination chemotherapy. Poly-lactic-co-glycolic acid scaffolds were fabricated by 3D printing to immobilize 5-fluorouracil and NVP-BEZ235. The implantable scaffolds significantly reduced the required drug dosages and ensured curative drug levels near tumor sites for prolonged period, while drug exposure to normal tissues was minimized. Moreover, long-term drug release was achieved, potentially allowing one-off implantation and, thus, a major reduction in the frequency of drug administration. This drug-loaded scaffold has great potential in anti-tumor treatment, possibly paving the way for precise, effective, and harmless cancer therapy.
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http://dx.doi.org/10.1016/j.biomaterials.2019.119618DOI Listing
February 2020

E-Jet 3D-Printed Scaffolds as Sustained Multi-Drug Delivery Vehicles in Breast Cancer Therapy.

Pharm Res 2019 Nov 18;36(12):182. Epub 2019 Nov 18.

College of Biology, Hunan University, Changsha, 410082, Hunan, China.

Purpose: Combination chemotherapy is gradually receiving more attention because of its potential synergistic effect and reduced drug doses in clinical application. However, how to precisely control drug release dose and time using vehicles remains a challenge. This work developed an efficient drug delivery system to combat breast cancer, which can enhance drug effects despite reducing its concentration.

Methods: Controlled-release poly-lactic-co-glycolic acid (PLGA) scaffolds were fabricated by E-jet 3D printing to deliver doxorubicin (DOX) and cisplatin (CDDP) simultaneously.

Results: This drug delivery system allowed the use of a reduced drug dosage resulting in a better effect on the human breast cancer cell apoptosis and inhibiting tumor growth, compared with the effect of each drug and the two drugs administrated without PLGA scaffolds. Our study suggested that DOX-CDDP-PLGA scaffolds could efficiently destroy MDA-MB-231 cells and restrain tumor growth.

Conclusions: The 3D printed PLGA scaffolds with their time-programmed drug release might be useful as a new multi-drug delivery vehicle in cancer therapy, which has a potential advantage in a long term tumor cure and prevention of tumor recurrence.
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http://dx.doi.org/10.1007/s11095-019-2687-3DOI Listing
November 2019

Ultra-compact dual-polarization silicon mode-order converter.

Opt Lett 2019 Sep;44(17):4179-4182

The mode-order converter is a building block in a multimode optical transmission and switching system. It can be used for switching signals carried on different mode channels. However, such devices constructed by conventional structures can commonly accomplish one mode-order conversion process. It is because the phase-matching condition, which is utilized by a majority of designs, can usually be fulfilled between only one mode pair for specific device geometry. In this Letter, we propose a dual-polarization mode-order converter referring to the concept of a silicon planar metasurface. It can realize mode-order conversions on transverse electrical and transverse magnetic polarizations in parallel. In order to verify our concept, we design and experimentally demonstrate a prototype that can realize conversions from TE to TE and from TM to TM simultaneously. The footprint is 4  μm×1.6  μm. The measured insertion losses for both polarizations are smaller than 2.3 dB, and the crosstalk is lower than -11.5  dB within the wavelength range of 1525-1565 nm. We envision that the device can be a building block in polarization and mode multiplexed optical switching systems and endow the systems with simpler structure and a more compact footprint.
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http://dx.doi.org/10.1364/OL.44.004179DOI Listing
September 2019

The topography of fibrous scaffolds modulates the paracrine function of Ad-MSCs in the regeneration of skin tissues.

Biomater Sci 2019 Sep;7(10):4248-4259

College of Biology, Hunan University, Changsha, Hunan, China410082.

Injuries to the skin are common in daily life, and a certain type or size of defect is not easily restored using conventional dressings or naturally. The repair of these defects requires restoration of function in regenerated tissues. In this study, a tissue engineered skin was designed and fabricated using a bio-3D printing system. Polycaprolactone and bacterial cellulose comprised the scaffold, due to their excellent biocompatibility and multifunctionality. Adipose-derived mesenchymal stem cells (Ad-MSCs) were seeded onto the scaffold to functionalize it as an artificial skin. The finished artificial skin had mechanical properties similar to that of natural skin, and its fibrous structure providing a unique micro-environment that could regulate the paracrine function of the Ad-MSCs. This effect could be greatly increased by changes in the characteristics of the biomaterials. The artificial skin exhibited high biological activity, strong induction of cell recruitment, migration, growth and up-regulation of gene expression of relevant factors, resulting in excellent wound healing characteristics. This study clarified novel design aspects of cell-material interactions in which the topographical characteristics of materials can be further developed to establish cell signaling or communication networks that take advantage of the paracrine actions of Ad-MSCs to promote specific tissue regeneration or repair characteristics.
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http://dx.doi.org/10.1039/c9bm00939fDOI Listing
September 2019

Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms.

Int J Nanomedicine 2019 4;14:937-950. Epub 2019 Feb 4.

College of Biology, Hunan University, Changsha, Hunan 410082, China,

Background: Skeletal muscle tissue engineering often involves the prefabrication of muscle tissues in vitro by differentiation and maturation of muscle precursor cells on a platform which provides an environment that facilitates the myogenic differentiation of the seeded cells.

Methods: Poly lactic-co-glycolic acid (PLGA) 3D printed scaffolds, which simulate the highly complex structure of extracellular matrix (ECM), were fabricated by E-jet 3D printing in this study. The scaffolds were used as platforms, providing environment that aids in growth, differentiation and other properties of C2C12 myoblast cells.

Results: The C2C12 myoblast cells grown on the PLGA 3D printed platforms had enhanced cell adhesion and proliferation. Moreover, the platforms were able to induce myogenic differentiation of the myoblast cells by promoting the formation of myotubes and up-regulating the expressions of myogenic genes (MyHC and MyOG).

Conclusion: The fabricated 3D printed platforms have excellent biocompatibility, thereby can potentially be used as functional cell culture platforms in skeletal tissue engineering and regeneration.
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http://dx.doi.org/10.2147/IJN.S193624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366362PMC
March 2019

A High Accuracy Time-Reversal Based WiFi Indoor Localization Approach with a Single Antenna.

Sensors (Basel) 2018 Oct 12;18(10). Epub 2018 Oct 12.

Guangdong Provincial Key Laboratory of Short-Range Wireless Detection and Communication, School of Electronic and Information Engineering, South China University of Technology, Guangzhou 510640, China.

In this paper, we study the influence of multipath magnitude, bandwidth, and communication link number on the performance of the existing time-reversal (TR) based fingerprinting localization approach and find that the localization accuracy deteriorates with a limited bandwidth. To improve the localization performance, by exploiting two unique location-specified signatures extracted from Channel State Information (CSI), we propose a high accuracy TR fingerprint localization approach, HATRFLA. Furthermore, we employ a density-based spatial clustering algorithm to minimize the storage space of the fingerprint database by adaptively selecting the optimal number of fingerprints for each location. Experimental results confirm that the proposed approach can efficiently mitigate accuracy deterioration caused by a limited bandwidth and consequently, achieve higher accuracy compared with the existing TR localization approach.
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http://dx.doi.org/10.3390/s18103437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210306PMC
October 2018

In Vitro Study of Colon Cancer Cell Migration Using E-Jet 3D Printed Cell Culture Platforms.

Macromol Biosci 2018 11 5;18(11):e1800205. Epub 2018 Sep 5.

College of Biology, Hunan University, Changsha, Hunan, 410082, China.

The development of accurate and predictive in vitro experimental models of human tumors consistent with in vivo tumor microenvironments has garnered great attention in modern cancer research. 3D scaffolds are fabricated in this study by E-jet 3D printing with the aim of replicating the functionalities of tumor microenvironments in vitro which could be applicable as screening platforms for novel therapeutic strategies. Tumor protein 53 (p53) plays an important role in penetration and migration in 2D cell culture. However, whether or not p53 has the same function in 3D cell culture and the underlying mechanisms are poorly understood. Results show that p53 deletion significantly decreases the speed of migration and proliferation of cancer cells within 3D environments. This study unveils aspects of cancer cell motility and migration and the steps involved in subsequent cancer metastases, which provides a new perspective and platform for the research of tumor metastasis therapy.
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http://dx.doi.org/10.1002/mabi.201800205DOI Listing
November 2018

Triple-Layer Vascular Grafts Fabricated by Combined E-Jet 3D Printing and Electrospinning.

Ann Biomed Eng 2018 Sep 29;46(9):1254-1266. Epub 2018 May 29.

College of Biology, Hunan University, Changsha, 410082, Hunan, China.

Small-diameter tissue-engineered vascular grafts are urgently needed for clinic arterial substitute. To simulate the structures and functions of natural blood vessels, we designed a novel triple-layer poly(ε-caprolactone) (PCL) fibrous vascular graft by combining E-jet 3D printing and electrospinning techniques. The resultant vascular graft consisted of an interior layer comprising 3D-printed highly aligned strong fibers, a middle layer made by electrospun densely fibers, and an exterior structure composed of mixed fibers fabricated by co-electrospraying. The biocompatible triple-layer graft was used for in vivo implantation, and results demonstrated that the longitudinally-aligned fibers within the lumen of the graft could enhance the proliferation and migration of endothelial cells, while maintained good mechanical properties. The exterior layer provided a pathway that encouraged cells to migrate into the scaffold after implantation. This experimental graft overcame the limitations of conventionally electrospun vascular grafts of inadequate porosity and lowly cell penetration. The unique structure of the triple-layer vascular graft promoted cell growth and infiltration in vivo, thus provided an encouraging substitute for in situ tissue engineering.
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http://dx.doi.org/10.1007/s10439-018-2065-zDOI Listing
September 2018