Publications by authors named "Haoran Zhang"

290 Publications

Epidemic versus economic performances of the COVID-19 lockdown in Japan: A Mobility Data Analysis.

Cities 2021 Oct 22:103502. Epub 2021 Oct 22.

School of Business, Society & Engineering, Mälardalen University, SE-72123 Västerås, Sweden.

Lockdown measures have been a "panacea" for pandemic control but also a violent "poison" for economies. Lockdown policies strongly restrict human mobility but mobility reduce does harm to economics. Governments meet a thorny problem in balancing the pros and cons of lockdown policies, but lack comprehensive and quantified guides. Based on millions of financial transaction records, and billions of mobility data, we tracked spatio-temporal business networks and human daily mobility, then proposed a high-resolution two-sided framework to assess the epidemiological performance and economic damage of different lockdown policies. We found that the pandemic duration under the strictest lockdown is less about two months than that under the lightest lockdown, which makes the strictest lockdown characterize both epidemiologically and economically efficient. Moreover, based on the two-sided model, we explored the spatial lockdown strategy. We argue that cutting off intercity commuting is significant in both epidemiological and economical aspects, and finally helped governments figure out the Pareto optimal solution set of lockdown strategy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cities.2021.103502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531026PMC
October 2021

Corrigendum to "A new perspective of triptolide-associated hepatotoxicity: Liver hypersensitivity upon LPS stimulation" [Toxicology 414 (2019) 45-56].

Toxicology 2021 Oct 8:152966. Epub 2021 Oct 8.

Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing, 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, 21009, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tox.2021.152966DOI Listing
October 2021

Source-tracking of the Chinese Chikungunya viruses suggests that Indian subcontinent and Southeast Asia act as major hubs for the recent global spread of Chikungunya virus.

Virol J 2021 Oct 11;18(1):203. Epub 2021 Oct 11.

The Chinese PLA Center for Disease Control and Prevention, 20 DongDa Street, Fengtai District, Beijing, 100071, China.

Background: Chikungunya fever, caused by the Chikungunya virus (CHIKV), has become a major global health concern, causing unexpected large outbreaks in Africa, Asia, Europe, and the Americas. CHIKV is not indigenous to China, and its origin in the country is poorly understood. In particular, there is limited understanding of the recent global spread of CHIKV in the context of the CHIKV epidemic.

Methods: Here we investigated a novel Chikungunya patient who came from Myanmar to China in August, 2019. Direct genome sequencing was performed via combined MinION sequencing and BGISEQ-500 sequencing. A complete CHIKV genome dataset, including 727 CHIKV genomes retrieved from GenBank and the genome sequenced in this study, was constructed. An updated and comprehensive phylogenetic analysis was conducted to understand the virus's origin, evolution, transmission routes and genetic adaptation.

Results: All globally distributed CHIKV genomes were divided into West Africa, East/Central/South African and Asian genotypes. The genome sequenced in this study was located in the Indian Ocean lineage, and was closely related to a strain isolated from an Australian patient who returned from Bangladesh in 2017. A comprehensive phylogenetic analysis showed that the Chinese strains mainly originated from the Indian subcontinent and Southeast Asia. Further analyses indicated that the Indian subcontinent and Southeast Asia may act as major hubs for the recent global spread of CHIKV, leading to multiple outbreaks and epidemics. Moreover, we identified 179 distinct sites, including some undescribed sites in the structural and non-structural proteins, which exhibited apparent genetic variations associated with different CHIKV lineages.

Conclusions: Here we report a novel CHIKV isolate from a chikungunya patient who came from Myanmar to China in 2019, and summarize the source and evolution of Chinese CHIKV strains. Our present findings provide a better understanding of the recent global evolution of CHIKV, highlighting the urgent need for strengthened surveillance against viral diversity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12985-021-01665-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507386PMC
October 2021

Observation-Based Mercury Export from Rivers to Coastal Oceans in East Asia.

Environ Sci Technol 2021 10 7;55(20):14269-14280. Epub 2021 Oct 7.

Ministry of Education Laboratory of Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China.

Globally, the consumption of coastal fish is the predominant source of human exposure to methylmercury, a potent neurotoxicant that poses health risks to humans. However, the relative importance of riverine inputs and atmospheric deposition of mercury into coastal oceans remains uncertain owing to a lack of riverine mercury observations. Here, we present comprehensive seasonal observations of riverine mercury and methylmercury loads, including dissolved and particulate phases, to East Asia's coastal oceans, which supply nearly half of the world's seafood products. We found that East Asia's rivers annually exported 95 ± 29 megagrams of mercury to adjacent seas, 3-fold greater than the corresponding atmospheric deposition. Three rivers alone accounted for 71% of East Asia's riverine mercury exports, namely: Yangtze, Yellow, and Pearl rivers. We further conducted a metadata analysis to discuss the mercury burden on seawater and found that riverine export, combined with atmospheric deposition and terrestrial nutrients, quantitatively elevated the levels of total, methylated, and dissolved gaseous mercury in seawater by an order of magnitude. Our observations support that massive amounts of riverine mercury are exported to coastal oceans on a continental scale, intensifying their spread from coastal seawater to the atmosphere, marine sediments, and open oceans. We suggest that the impact of mercury transport along the land-ocean aquatic continuum should be considered in human exposure risk assessments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.1c03755DOI Listing
October 2021

Implementation of a twin-beam state-based clock synchronization system with dispersion-free HOM feedback.

Opt Express 2021 Aug;29(18):28607-28618

In the field of clock synchronization, the application of frequency-entangled source is a promising direction to improve accuracy and security. In this paper, we analyze the performance of the twin-beam state and the difference-beam state using a practical second-order interference-based scheme. The advantages of the twin-beam state are pointed out especially for the dispersion-free property of HOM interference in a long-distance clock transfer. With the introduction of dispersion-compensated material, our experimental system based on a twin-beam state achieves a clock accuracy at 4 ps with a time offset precision of 1.8 ps over 10 s acquisition time while the time deviation is 0.15 ps over an averaging time of 5500 s in a 22 km-long transmission. These properties exhibit a leading position compared with the current clock synchronization system using the same theoretical scheme and also competitive among the implementations using other second-order interference-based schemes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.434386DOI Listing
August 2021

Focusing characteristics of linearly polarized Lorentz-Gaussian vortex beams with sinusoidal phase modulation.

Appl Opt 2021 Jul;60(21):6128-6134

Based on the vector diffraction theory, this paper investigates the focusing properties of linearly polarized Lorentz-Gaussian vortex beams with sinusoidal phase modulation and discusses the focused light intensity under different parameters in detail. Results show that the focus pattern in the horizontal direction at the focal region can be compressed by increasing the relative Gaussian parameter . As the relative Lorentz parameter increases, the focus pattern will separate in the vertical direction of the optical field. With the topological charge number increases, a special tunable optical dark trap focusing mode can be obtained. Through changing the sinusoidal modulation parameter , the regular trilateral, quadrangle, pentagon, and hexagon shapes of the focusing mode can be correspondingly constructed. Besides, on increasing propagation distance , the focusing mode in the near focusing region gradually extends outside and always exhibits hexagon-shaped patterns, which reflects that this special focusing mode has a good stability. In addition, the optical gradient force distributions and the field intensity distributions in the longitudinal plane are also investigated to illuminate the applications of these alterable focal patterns. Those novel, to the best of our knowledge, findings may be helpful in applications such as optical manipulation, optical focusing, and imaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/AO.430788DOI Listing
July 2021

A novel Ka-band coaxial transit time oscillator with internal extraction.

Rev Sci Instrum 2021 Sep;92(9):094704

College of Advanced Interdisciplinary Studies, National University of Defense Technology, Changsha 410073, People's Republic of China.

In this paper, a Ka-band coaxial transit time oscillator (TTO) with internal extraction is proposed. Particle-in-cell simulation of this oscillator is performed to obtain results as follows: under the conditions of a diode voltage of 459 kV, current of 3.9 kA, and guiding magnetic field of 0.5 T, microwaves with an output power of 0.75 GW and a frequency of 31.4 GHz can be achieved with an efficiency of 42% and a saturation time of nearly 25 ns. Moreover, the asymmetric mode competition is suppressed in the preliminary experiments. The study of a Ka-band TTO aims to extend the working frequency of high power microwave sources to a higher level. Such a device has three merits. First, it implements high power and high efficiency. Second, the internal extraction of the microwave output decreases the over-mode ratio in the microwave extraction region. Third, the over-mode ratio of the internal extraction is smaller compared with the external extraction, which can effectively suppress asymmetric mode competition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1063/5.0062144DOI Listing
September 2021

Melatonin ameliorates trimethyltin chloride-induced cardiotoxicity: The role of nuclear xenobiotic metabolism and Keap1-Nrf2/ARE axis-mediated pyroptosis.

Biofactors 2021 Sep 27. Epub 2021 Sep 27.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, P.R. China.

Trimethyltin chloride (TMT) is a stabilizer for polyvinyl chloride plastics that causes serious health hazards in nontarget organisms. Melatonin (MT) exhibits powerful protective effects in cardiac diseases. As a new environmental pollutant, TMT-induced cardiotoxicity and the protective effects of MT remain unclear. To explore this, the mice were treated with TMT (2.8 mg/kg) and/or MT (10 mg/kg) for 7 days. Firstly, the histopathological and ultrastructural evaluation showed that TMT induced cardiac damage, tumescent rupture and nuclear pyknosis. Moreover, TMT elevated the expressions of pyroptosis genes NLRP3, ASC and Cas1 and inflammation factors IL-6, IL-17 and TNFα. Secondly, TMT reduced antioxidant enzymes (GSH, CAT and T-AOC) via decreasing the expression of genes associated with the Keap1-Nrf2/ARE pathway to increase oxidative stress. Thirdly, TMT decreased the expression of genes associated with the ARE-driven drug metabolizing enzymes (DMEs), including Akr7a3, Akr1b8, and Akr1b10. Besides, TMT upregulated the mRNA expression of nuclear Xenobiotic metabolism on cytochrome P450s enzymes via increasing the expression of CAR, PXP, and AHR genes. Furthermore, MT treatment mitigated the aforementioned adverse changes induced by TMT. Overall, these results demonstrated that TMT caused pyroptosis and inflammation to aggravate cardiac damage via inducing excessive oxidative stress, imbalance of DMEs homeostasis, and nuclear Xenobiotic metabolism disorder, which could be alleviated by MT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/biof.1787DOI Listing
September 2021

Effect of anesthesia on the expression of clock gene Clock and Bmal1 in New Zealand rabbits.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Aug;46(8):809-813

Department of Anesthesiology and Perioperative Medicine, Henan Provincial People's Hospital, Zhengzhou 450003, China.

Objectives: To evaluate the effect of anesthetic drugs on the expression of circadian gene Clock and Bmal1 in the brain of New Zealand rabbits, and to explore their change pattern.

Methods: A total of 90 New Zealand rabbits were randomly divided into 5 groups (=18 in each group): A normal saline group (1 mL/h saline, group S), a propofol group [600 µg/(kg·min) propofol, 1 mL/h, group P], a 10% lipid group (1 mL/h lipid, group F), a dexmedetomidine group [1 µg/(kg·min) dexmedetomidine, 1 mL/h, group D), a sevoflurane (SEV) group (2.5% SEV, SEV group). Inhaled and intravenous anesthetic drugs were stopped after 24 h. Experimental animals were killed at 0, 24, and 72 h after anesthesia, and their brain tissues were isolated. Western blotting was performed to measure the Clock and Bmal1 protein expression in the brain of rabbits.

Results: At 0 and 24 h after anesthesia, compared with the group S, the levels of Clock and Bmal1 proteins were decreased significantly in the group P, the group D, and the group SEV (all <0.05). At 72 h after anesthesia, compared with the group S, the levels of Clock and Bmal1 proteins showed no significant changes in the group P, the group D, and the SEV group (all >0.05). Compared with the group S, the levels of Clock and Bmal1 proteins at all time points showed no significant changes in the group F (all >0.05).

Conclusions: Anesthetic SEV, propofol, and dexmedetomidine can inhibit the expression of clock gene Clock and Bmal1 protein in the brain fissues of the New Zealand rabbits, and the suppression effect continues for at least 24 h after anesthesia, whereas the suppression decreases significantly at 72 h after anesthesia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200755DOI Listing
August 2021

Chirality Affecting Reaction Dynamics of HgS Nanostructures Simultaneously Visualized in Real and Reciprocal Space.

ACS Nano 2021 Oct 23;15(10):16255-16265. Epub 2021 Sep 23.

Center for Ultrafast Science and Technology, School of Chemistry and Chemical Engineering, and School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240, China.

Chirality involved reactions enable to probe features in the fields of asymmetric synthesis and catalysis, which allow to gain insight into the fundamental mechanisms of topochemically controlled reactions. However, observation of the chirality-associated reaction dynamics with simultaneous structural determination of microscopic features has been lacking. Here, we report the direct visualization of the electron-beam-stimulated reaction dynamics of HgS nanostructures with chiral and achiral morphologies simultaneously in both real and reciprocal space. Under the electron-beam excitation of HgS nanostructures, the formation and evaporation dynamics of Hg nanodroplets were vividly pictured, while the reciprocal space imaging revealed the structural transformation from monocrystalline to polycrystalline. Interestingly, such induced changes were size dependent, which were slowed when involving the chirality in the nanostructures. The finding offers a fundamental understanding of topochemically controlled reaction mechanisms and holds promise of tuning asymmetric synthesis for catalysis-related applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c05243DOI Listing
October 2021

Metagenomics insights into the selective inhibition of NOB and comammox by phenacetin: Transcriptional activity, nitrogen metabolism and mechanistic understanding.

Sci Total Environ 2021 Sep 3;803:150068. Epub 2021 Sep 3.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Faculty of Environment and Life Sciences, Beijing University of Technology, Beijing 100124, China.

Phenacetin (PNCT), a common antipyretic and analgesic drug, is often used to treat fever and headache. However, the effect of PNCT on nitrifiers in wastewater treatment processes remains unclear. The practicability of attaining partial nitrification (PN) through inhibitor-PNCT was investigated in this study. The optimal treatment conditions of soaking once for 18 h with 2.50 × 10 g PNCT/(g MLSS) were applied to the PN stability experiment. The results showed that ammonia oxidation activity recovered quickly after 3 cycles of operation, while nitrite oxidation activity was suppressed steadily. In addition, average ammonium removal efficiency and nitrite accumulation ratio during 138 cycles could reach 94.94% and 85.38%, respectively. Complimentary DNA high-throughput sequencing and oligotyping analysis showed that the activity of Nitrosomonas would gradually surpass Nitrospira after PNCT treatment only once. The decrease of Nitrospira activity was accompanied by the simplification of oligotypes after PNCT treatment, while Nitrosomonas could adapt to PNCT stress by reducing the differences between oligotypes. Metagenomics revealed that the decrease in the number of NXR in the nitrogen metabolism pathways was the key reason for achieving PN. The potential mechanisms might be that the dominant nitrite-oxidizing bacteria and complete ammonia oxidizers were bio-killed by PNCT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.150068DOI Listing
September 2021

Biocompatible Ruthenium Single-Atom Catalyst for Cascade Enzyme-Mimicking Therapy.

ACS Appl Mater Interfaces 2021 Sep 14;13(38):45269-45278. Epub 2021 Sep 14.

Department of Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei 230026, China.

Rationally constructing single-atom enzymes (SAEs) with superior activity, robust stability, and good biocompatibility is crucial for tumor therapy but still remains a substantial challenge. In this work, we adopt biocompatible carbon dots as the carrier material to load Ru single atoms, achieving Ru SAEs with superior multiple enzyme-like activity and stability. Ru SAEs behave as oxidase, peroxidase, and glutathione oxidase mimics to synchronously catalyze the generation of reactive oxygen species (ROS) and the depletion of glutathione, thus amplifying the ROS damage and finally causing the death of cancer cells. Notably, Ru SAEs exhibit excellent peroxidase-like activity with a specific activity of 7.5 U/mg, which surpasses most of the reported SAEs and is 20 times higher than that of Ru/C. Theoretical results reveal that the electrons of the Ru 4d orbital in Ru SAEs are transferred to O atoms in HO and then efficiently activate HO to produce OH. Our work may provide some inspiration for the design of SAEs for cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c12706DOI Listing
September 2021

Deep learning classification of cervical dysplasia using depth-resolved angular light scattering profiles.

Biomed Opt Express 2021 Aug 19;12(8):4997-5007. Epub 2021 Jul 19.

Department of Biomedical Engineering, Duke University, Durham, NC 27703, USA.

We present a machine learning method for detecting and staging cervical dysplastic tissue using light scattering data based on a convolutional neural network (CNN) architecture. Depth-resolved angular scattering measurements from two clinical trials were used to generate independent training and validation sets as input of our model. We report 90.3% sensitivity, 85.7% specificity, and 87.5% accuracy in classifying cervical dysplasia, showing the uniformity of classification of a/LCI scans across different instruments. Further, our deep learning approach significantly improved processing speeds over the traditional Mie theory inverse light scattering analysis (ILSA) method, with a hundredfold reduction in processing time, offering a promising approach for a/LCI in the clinic for assessing cervical dysplasia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/BOE.430467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407824PMC
August 2021

Efficacy and Safety of Individualized Schedule of Sunitinib by Drug Monitoring in Patients with Metastatic Renal Cell Carcinoma.

Cancer Manag Res 2021 31;13:6833-6845. Epub 2021 Aug 31.

Department of Urology, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.

Purpose: To investigate the survival benefit and safety of individualized schedules for sunitinib in patients with metastatic renal cell carcinoma (mRCC) through plasma concentration monitoring.

Methods: A total of 105 patients with mRCC were enrolled. The schedule was adjusted in two ways: therapeutic drug monitoring (TDM) and toxicity-adjusted schedule (TAS). One group of patients were without any schedule adjustment (maintained schedule, MAS). Progression-free survival (PFS), overall survival (OS), tumor response, and adverse events (AEs) were compared. The relationship between AEs and steady-state concentration or consecutive monitoring curves was explored. Further monitoring of individualized schedules was also conducted.

Results: Based on the plasma concentration, the schedules of 18 patients were adjusted in the TDM group. The schedules were adjusted in 37 patients due to severe AEs in the TAS group, while 50 patients were without any schedule adjustment. The median PFS and OS were better in the TDM group than the other two groups (p = 0.001 and p = 0.004, respectively). Univariate and multivariate analyses indicated that TDM could decrease the risk of death independently (p = 0.026). Moreover, the incidence of grades 3/4 AEs decreased from 88.9% to 33.3% in the TDM group (p = 0.001). Sunitinib concentration in 150-200ng/mL was regarded as a "transitional zone" due to severe AEs mainly happened when concentration elevated over it. After TDM, further plasma concentration monitoring indicated that individualized schedules enabled sunitinib concentration to fluctuate in a much safer range.

Conclusion: Treatment-related toxicities could be minimized through plasma concentration monitoring. Patients with adjusted schedules by therapeutic drug monitoring could achieve better survival benefits.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/CMAR.S327029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418361PMC
August 2021

Integrated exome and RNA sequencing of TFE3-translocation renal cell carcinoma.

Nat Commun 2021 09 6;12(1):5262. Epub 2021 Sep 6.

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.

TFE3-translocation renal cell carcinoma (TFE3-tRCC) is a rare and heterogeneous subtype of kidney cancer with no standard treatment for advanced disease. We describe comprehensive molecular characteristics of 63 untreated primary TFE3-tRCCs based on whole-exome and RNA sequencing. TFE3-tRCC is highly heterogeneous, both clinicopathologically and genotypically. ASPSCR1-TFE3 fusion and several somatic copy number alterations, including the loss of 22q, are associated with aggressive features and poor outcomes. Apart from tumors with MED15-TFE3 fusion, most TFE3-tRCCs exhibit low PD-L1 expression and low T-cell infiltration. Unsupervised transcriptomic analysis reveals five molecular clusters with distinct angiogenesis, stroma, proliferation and KRAS down signatures, which show association with fusion patterns and prognosis. In line with the aggressive nature, the high angiogenesis/stroma/proliferation cluster exclusively consists of tumors with ASPSCR1-TFE3 fusion. Here, we describe the genomic and transcriptomic features of TFE3-tRCC and provide insights into precision medicine for this disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-25618-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421377PMC
September 2021

The Association Between Mycobacteria-Specific Antigen-Induced Cytokines and Host Response to Latent Tuberculosis Infection Treatment in a Chinese Population.

Front Microbiol 2021 13;12:716900. Epub 2021 Aug 13.

NHC Key Laboratory of Systems Biology of Pathogens, Center for Tuberculosis Research, Institute of Pathogen Biology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Objectives: Exploring biomarkers monitoring latent tuberculosis infection (LTBI) treatment effectiveness would benefit optimizing the therapeutic regimen. This study aims to identify potential mycobacteria-specific antigen-induced cytokines associated with host responses to preventive treatment.

Methods: Based on a randomized controlled trial on LTBI treatment among individuals with chest radiography abnormalities suggestive of prior tuberculosis (TB), the dynamically changed cytokine levels in QuantiFERON-TB Gold In-Tube (QFT) supernatants were estimated during the treatment by bead-based multiplex assays and enzyme-linked immunosorbent assay.

Results: In total, 63 treated participants and 32 untreated controls were included in the study. The levels of 13 background-corrected mycobacteria-specific antigen-stimulated cytokines [basic fibroblast growth factor (FGF), growth-regulated oncogene (GRO)-α, interleukin (IL)-1α, IL-1ra, IL-12 (p70), stem cell factor (SCF), tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), IL-8, interferon (IFN)-α2, IL-5, IL-12 (p40), leukemia inhibitory factor (LIF), and IL-17A] were found to be statistically different between before and after treatment in treated participants, while no statistically differences were observed in untreated controls. Among these 13 cytokines, the level of IL-8 was significantly lower in the QFT reversed group than that in the non-reversed group ( = 0.028) among treated participants, while such a difference was not found for untreated controls ( = 0.292).

Conclusion: Our results suggested that the lower level of mycobacteria-specific antigen-induced IL-8 might be associated with the host's positive response to LTBI treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.716900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415003PMC
August 2021

The removal of antibiotic resistant bacteria and genes and inhibition of the horizontal gene transfer by contrastive research on sulfidated nanoscale zerovalent iron activating peroxymonosulfate or peroxydisulfate.

J Hazard Mater 2021 Aug 8;423(Pt A):126866. Epub 2021 Aug 8.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Faculty of Environment and Life Sciences, Beijing University of Technology, Beijing 100124, China.

Antibiotic resistant bacteria (ARB) and the antibiotic resistance genes (ARGs) dissemination via plasmid-mediated conjugation have attracted considerable attentions. In this research, sulfidated nanoscale zerovalent iron (S-nZVI)/peroxymonosulfate (PMS) and S-nZVI/peroxydisulfate (PDS) process were investigated to inactivate ARB (Escherichia coli DH5α with RP4 plasmid, Pseudomonas. HLS-6 contains sul1 and intI1 on genome DNA sequence). S-nZVI/PMS system showed higher efficiency than S-nZVI/PDS on ARB inactivation. Thus, the optimal condition 28 mg/L S-nZVI coupled with 153.7 mg/L (0.5 mM) PMS was applied to remove both intracellular ARGs (iARGs) and ARB. The oxidative damage of ARB cell was systemically studied by cell viability, intracellular Mg levels, the changes of extracellular and internal structure, integrity of cell walls and membranes and enzymatic activities. S-nZVI/PMS effectively inactivated ARB (~7.32 log) within 15 min. These effects were greatly higher than those achieved individually. Moreover, removal efficiencies of iARGs sul1, intI1 and tetA were 1.52, 1.79 and 1.56 log, respectively. These results revealed that S-nZVI and PMS have a synergistic effect against ARB and iARGs. The regrowth assays illustrated that the ARB were effectively inactivated. By verifying the inhibitory impacts of S-nZVI/PMS treatment on conjugation transfer, this work highlights a promising alternative technique for inhibiting the horizontal gene transfer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.126866DOI Listing
August 2021

The effect of WeChat-based training on improving the knowledge of tuberculosis management of rural doctors.

J Clin Tuberc Other Mycobact Dis 2021 Dec 11;25:100266. Epub 2021 Aug 11.

NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.

Background: In China, rural doctors played a crucial role in TB cases referral and management. The current study aimed to evaluate the effect of WeChat-based training program on improving the rural doctors' knowledge on TB management.

Methods: A One-year WeChat-based training was conducted among registered rural doctors from Zhongmu County, located in middle China, by means of releasing original contents (in forms of text, poster, video or cartoon) through WeChat subscription account (WeChat SA) once a week. Pre-and-post-training offline tests were hold using the same self-administered questionnaire to evaluate the training effect.

Results: A total of 467 rural doctor were included in the study. During the training, 60 original articles were posted through WeChat SA. With respect to the two tests, the median score increased from 50 (40.0-60.0) to 60 (53.0-70.0) (p < 0.001) after training. As compared with posters, the median readings were significantly higher for released contents in forms of text, video and cartoon (p < 0.001). Female's test performance improved better than male's. In addition, a positive relation was observed between education level and the test performance regardless of training.

Conclusions: Our results indicated that WeChat-based training improved the knowledge of rural doctors on TB management to a certain extent. It is worthy to explore more effective new media-based training methods to promote TB control in rural China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jctube.2021.100266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379341PMC
December 2021

Effects of Rhamnolipids on Growth Performance, Immune Function, and Cecal Microflora in Linnan Yellow Broilers Challenged with Lipopolysaccharides.

Antibiotics (Basel) 2021 Jul 24;10(8). Epub 2021 Jul 24.

Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, The Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, College of Animal Medicine, Zhejiang Agriculture & Forestry University, Hangzhou 311300, China.

This present study aimed to investigate the effects of rhamnolipids (RLS) on the growth performance, intestinal morphology, immune function, short-chain fatty acid content, and microflora community in broiler chickens challenged with lipopolysaccharides (LPS). A total of 450 broiler chickens were randomly allocated into three groups: basal diet with no supplement (NCO), basal diet with bacitracin (ANT), and basal diet with rhamnolipids (RLS). After 56 d of feeding, 20 healthy broilers were selected from each group, with half being intraperitoneally injected with lipopolysaccharides (LPS) and the other half with normal saline. Treatments with LPS were labelled LPS-NCO, LPS-ANT, and LPS-RLS, whereas treatments with normal saline were labelled NS-NCO, NS-ANT, and NS-RLS. LPS-challenged birds had lower jejunal villus height and higher crypt depth than unchallenged birds. LPS-RLS broilers had increased jejunal villus height and villus height/crypt depth ratio (V/C) but lower crypt depth than LPS-NCO. Dietary supplementation with RLS reduced the LPS-induced immunological stress. Compared with LPS-NCO, birds in LPS-RLS had lower concentrations of IL-1β, IL-6, and TNF-α. In LPS-challenged broilers, RLS and ANT increased the concentrations of IgA, IgM, and IgY compared with LPS-NCO. In LPS treatments, RLS enhanced the contents of acetic acid, butyrate, isobutyric acid, isovalerate, and valerate more than LPS-NCO birds. High-throughput sequencing indicated that RLS supplementation led to changes in the cecal microbial community of broilers. At the species level, was more abundant in NS-RLS than in NS-NCO broilers. In summary, RLS improved the growth performance and relative abundance of cecal microbiota and reduced the LPS-induced immunological stress in broiler chickens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/antibiotics10080905DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388811PMC
July 2021

The heterogeneity of intraductal carcinoma of the prostate is associated with different efficacy of standard first-line therapy for patients with metastatic castration-resistant prostate cancer.

Prostate 2021 Nov 26;81(15):1191-1201. Epub 2021 Aug 26.

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.

Background: To explore whether metastatic castration-resistant prostate cancer (mCRPC) patients with distinct intraductal carcinoma of the prostate (IDC-P) subtypes respond differently to abiraterone and docetaxel treatment.

Methods: We retrospectively analyzed data of 170 mCRPC patients receiving abiraterone or docetaxel as first-line therapy. PSA response, PSA progression-free survival (PSA-PFS), radiographic progression-free survival (rPFS), and overall survival (OS) were analyzed based on the presence of IDC-P and its subpatterns.

Results: IDC-P was confirmed in 91/170 (53.5%) patients. Among them 36/91 (39.6%) and 55/91 (60.4%) harbored IDC-P patterns 1 and 2, respectively. Patients with IDC-P pattern 1 shared similar clinical outcomes to those without IDC-P in both abiraterone and docetaxel treatment. However, against cases without IDC-P or with IDC-P pattern 1, patients with IDC-P pattern 2 had markedly poorer prognosis in either abiraterone (mPSA-PFS: 11.9 vs. 11.1 vs. 6.1 months, p < 0.001; mrPFS: 18.9 vs. 19.4 vs. 9.6 months, p < 0.001) or docetaxel (mPSA-PFS: 6.2 vs. 6.6 vs. 3.0 months, p < 0.001; mrPFS: 15.1 vs. 12.6 vs. 5.5 months, p < 0.001) treatment. For patients without IDC-P, docetaxel had comparable therapeutic efficacy with abiraterone. However, the efficacy of docetaxel was significantly inferior to abiraterone in patients with either IDC-P pattern 1 (mPSA-PFS: 6.6 vs. 11.1 months, p = 0.021; mrPFS: 12.6 vs. 19.4 months, p = 0.027) or pattern 2 (mPSA-PFS: 3.0 vs. 6.1 months, p = 0.003; mrPFS: 5.5 vs. 9.6 months, p = 0.007).

Conclusion: Compared to docetaxel, abiraterone exhibited better efficacy in patients with IDC-P of either pattern. However, IDC-P pattern 2 responded unsatisfactorily to either abiraterone or docetaxel therapy. Novel therapeutic strategies for IDC-P pattern 2 need further investigations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/pros.24215DOI Listing
November 2021

Self-enhanced and efficient removal of As(III) from water using Fe-Cu-Mn composite oxide under visible-light irradiation: Synergistic oxidation and mechanisms.

J Hazard Mater 2021 Aug 13;422:126908. Epub 2021 Aug 13.

State Key Laboratory of Urban Water Resource and Environment (SKLUWRE), School of Environment, Harbin Institute of Technology, Harbin 150090, China.

Here, we prepared a novel nanostructured Fe-Cu-Mn composite oxide (FCMO) adsorbent using an ultrasonic coprecipitation method. The maximum adsorption capacity of As(III) and As(V) reached 158.5 and 115.2 mg/g under neutral conditions, respectively. The effects of several environmental factors (coexisting ions, solution pH, etc.) on the removal of inorganic arsenic using FCMO were studied through batch experiments. The results showed that except for PO and high initial pH, it was not significantly affected by ionic strength and other existing anions, implying a higher selectivity and adaptability. Combined with EPR, FTIR, and XPS analysis, we concluded that the Cu component and the reactive oxygen species (ROS) it generates played a decisive role in maintaining the stability of the redox cycle between Mn(IV)/Mn(III)/Mn(II) and enhancing the oxidation efficiency of As(III). Meanwhile, the adsorption mechanism of As(V) was mainly through the replacement of the FCMO surface -OH to form stable inner-sphere arsenic complexes, while the removal mechanism of As(III) may involve the process of synergistic oxidation and chemisorption coupling. Additionally, the effective removal of As from the simulated As-contaminated water and its satisfactory reuse performance make FCMO adsorbents favorable candidates for the removal of As-contaminated water in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.126908DOI Listing
August 2021

Comparing rice production systems in China: Economic output and carbon footprint.

Sci Total Environ 2021 Oct 25;791:147890. Epub 2021 May 25.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

In recent years, many rotational and integrated rice production systems coupled with several greenhouse gas (GHG) emissions mitigation practices have been developed and adopted for demand of low carbon production. However, there have been only few studies about comparisons on the balance between high production and mitigation of GHG emissions in different rice production systems. We therefore aimed to evaluate economic output and carbon footprint of different rice production systems, based on several long-term experiments conducted by our lab. CH and NO emission were measured by the same static chamber/gas chromatogram measurement procedure in different rice production systems, including rice-fallow, rice-rapeseed, rice-wheat, double rice, and integrated rice-crayfish production system. Then, we applied the DeNitrification DeComposition model to simulate CH and NO emission over different years under the same condition for comparison. Carbon footprint was calculated following the process-based life cycle assessment (PLCA) methodology. The economic benefit of rice production systems was assessed by cost-benefit analysis. According to the analysis, the double-rice production system exhibited the highest intensity of carbon footprint (ICF = 4.14 kg CO-eq yuan), rain-fed treatment in the rice-rapeseed system had the lowest (ICF = 0.68 kg CO-eq yuan). The intensity of carbon footprint in different treatments in the integrated rice-crayfish production system was around 0.8 kg CO-eq yuan. Overall, the results of this case study suggest: (1) the proposed practices in different rice production systems are no straw returning (rice-fallow), no-tillage without straw returning (rice-wheat), rain-fed farming (rice-rapeseed), no insect and no inoculation (double rice), and feeding with straw returning (rice-crayfish); (2) rotational and integrated systems can achieve high net output with low carbon emission; (3) reducing the amount of nitrogenous fertilizer application is the most important and effective GHG mitigation practice for rotational systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.147890DOI Listing
October 2021

Photodepositing CdS on the Active Cyano Groups Decorated g-C N in Z-Scheme Manner Promotes Visible-Light-Driven Hydrogen Evolution.

Small 2021 Oct 16;17(39):e2102699. Epub 2021 Aug 16.

State Key Laboratory of Marine Resource Utilization in South China Sea, School of Materials Science and Engineering, Hainan University, Haikou, 570228, P. R. China.

g-C N /CdS heterojunctions are potential photocatalysts for hydrogen production but their traditional type-II configuration generally leads to weak oxidative and reductive activity. How to construct the novel Z-scheme g-C N /CdS counterparts to address this issue remains a great challenge in this field. In this work, a new direct Z-scheme heterojunction of defective g-C N /CdS is designed by introducing cyano groups (NC-) as the active bridge sites. Experimental observations in combination with density functional theory (DFT) calculations reveal that the unique electron-withdrawing feature of cyano groups in the defective g-C N /CdS heterostructure can endow this photocatalyst with numerous advantageous properties including high light absorption ability, strong redox performance, satisfactory charge separation efficiency, and long lifetime of charge carriers. Consequently, the resultant photocatalytic system exhibits more active performance than CdS and g-C N under visible light and reaches an excellent hydrogen evolution rate of 1809.07 µmol h g , which is 6.09 times higher than pristine g-C N . Moreover, the defective g-C N /CdS photocatalyst maintains good stability after 40 h continuous test. This work provides new insights into design and construction of Z-scheme heterojunctions for regulating the visible-light-induced photocatalytic activity for H evolution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202102699DOI Listing
October 2021

Versatile Printing of Substantial Liquid Cells for Efficiently Imaging Liquid-Phase Dynamics.

Nano Lett 2021 08 13;21(16):6882-6890. Epub 2021 Aug 13.

Center for Ultrafast Science and Technology, School of Physics and Astronomy, and School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

Through its ability to image liquid-phase dynamics at nano/atomic-scale resolution, liquid-cell electron microscopy is essential for a wide range of applications, including wet-chemical synthesis, catalysis, and nanoparticle tracking, for which involved structural features are critical. However, statistical investigations by usual techniques remain challenging because of the difficulty in fabricating substantial liquid cells with appreciable efficiency. Here, we report a general approach for efficiently printing huge numbers of ready-to-use liquid cells (∼9000) within 30 s by electrospinning, with the unique feature of statistical liquid-phase studies requiring only one experimental time slot. Our solution efficiently resolves a complete transition picture of bubble evolution and also the induced nanoparticle motion. We statistically quantify the effect of the electron dose rate on the bubble variation and conclude that the bubble-driven nanoparticle motion is a ballistic-like behavior insignificant to morphological asymmetries. The versatile approach here is critical for statistical research, offering great opportunities in liquid-phase-associated dynamic studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.1c01901DOI Listing
August 2021

TiO/Chlorophyll S-Scheme Composite Photocatalyst with Improved Photocatalytic Bactericidal Performance.

ACS Appl Mater Interfaces 2021 Aug 13;13(33):39446-39457. Epub 2021 Aug 13.

Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, P. R. China.

Step-scheme (S-scheme) photocatalysts have been proposed for highly efficient charge separation and strong redox activity in the photocatalysis field. Here, we reported a facile strategy to obtain the S-scheme heterojunction composite TiO/chlorophyll (Chl). The S-scheme heterojunction enables the significant improvement of electron transfer efficiency at the interfacial heterojunction of TiO/Chl. Also, the lifted conduction band and valence band of TiO/Chl resulted in more than 1.61 times generation of reactive oxidizing species, compared to that of bare TiO. In addition, TiO/Chl was applied as a photocatalytic bactericidal material to fabricate commercial masks for prolonged life span of the mask. The TiO/Chl-coated mask filter exhibited excellent bactericidal effect on under light illumination (2.94 × 10 cfu were killed by 1 cm coated mask filters within illumination of 3 h), while commercial mask filters showed no bactericidal effect. After three circulation-sterilization tests, the TiO/Chl-made mask filter maintained the initial bactericidal effect, which greatly extended the life span of the mask that presents a promising strategy to alleviate the supply stress of masks.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c10892DOI Listing
August 2021

Development and optimization of a microbial co-culture system for heterologous indigo biosynthesis.

Microb Cell Fact 2021 Aug 4;20(1):154. Epub 2021 Aug 4.

Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.

Background: Indigo is a color molecule with a long history of being used as a textile dye. The conventional production methods are facing increasing economy, sustainability and environmental challenges. Therefore, developing a green synthesis method converting renewable feedstocks to indigo using engineered microbes is of great research and application interest. However, the efficiency of the indigo microbial biosynthesis is still low and needs to be improved by proper metabolic engineering strategies.

Results: In the present study, we adopted several metabolic engineering strategies to establish an efficient microbial biosynthesis system for converting renewable carbon substrates to indigo. First, a microbial co-culture was developed using two individually engineered E. coli strains to accommodate the indigo biosynthesis pathway, and the balancing of the overall pathway was achieved by manipulating the ratio of co-culture strains harboring different pathway modules. Through carbon source optimization and application of biosensor-assisted cell selection circuit, the indigo production was improved significantly. In addition, the global transcription machinery engineering (gTME) approach was utilized to establish a high-performance co-culture variant to further enhance the indigo production. Through the step-wise modification of the established system, the indigo bioproduction reached 104.3 mg/L, which was 11.4-fold higher than the parental indigo producing strain.

Conclusion: This work combines modular co-culture engineering, biosensing, and gTME for addressing the challenges of the indigo biosynthesis, which has not been explored before. The findings of this study confirm the effectiveness of the developed approach and offer a new perspective for efficient indigo bioproduction. More broadly, this innovative approach has the potential for wider application in future studies of other valuable biochemicals' biosynthesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12934-021-01636-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336371PMC
August 2021

Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids.

Nat Biomed Eng 2021 Aug 2. Epub 2021 Aug 2.

State Key Laboratory of Natural and Biomimetic Drugs, Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing, China.

Approximately 11% of monogenic diseases involve nonsense mutations that are caused by premature termination codons. These codons can in principle be read-through via the site-specific incorporation of unnatural amino acids to generate full-length proteins with minimal loss of function. Here we report that aminoacyl-tRNA-synthase-tRNA pairs specific for the desired unnatural amino acids can be used to read through a nonsense mutation in the dystrophin gene. We show partial restoration of dystrophin expression in differentiated primary myoblasts (from a mdx mouse model and a patient with Duchenne muscular dystrophy), and restoration of muscle function in two mouse models: mdx mice, via viral delivery of the engineered tRNA-synthase-tRNA pair intraperitoneally or intramuscularly and of the associated unnatural amino acid intraperitoneally; and mice produced by crossing mdx mice and transgenic mice with a chromosomally integrated pair, via intraperitoneal delivery of the unnatural amino acid. The incorporation of unnatural amino acids to restore endogenous protein expression could be explored for therapeutic use.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41551-021-00774-1DOI Listing
August 2021

Cadmium exposure triggers oxidative stress, necroptosis, Th1/Th2 imbalance and promotes inflammation through the TNF-α/NF-κB pathway in swine small intestine.

J Hazard Mater 2022 01 20;421:126704. Epub 2021 Jul 20.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China. Electronic address:

Cadmium (Cd) is a toxic environmental pollutant and induces toxic effects to organism. Nevertheless, the mechanism of Cd-induced toxicity in swine remains obscure. To explore this, 10 healthy 6-week-old weaned swine were placed into two groups stochastically, the Cd group was treated with a commercial diet containing 20 mg/kg Cd for 40 days. The results of histopathological and ultrastructural observations showed typical necrosis features and inflammatory cell infiltration in Cd group. Excessive Cd suppressed T-AOC and SOD activities, increased MDA content and ROS levels. Cd diet elevated the expression of RIPK1, RIPK3, and MLKL to activate the RIPK3-dependent necroptosis pathway. Results of Th1 and Th2 cytokines indicated that the levels of IL-4, IL-6 and IL10 was increased, while the level of IFN-γ was decreased, illustrating Th1/Th2 immune imbalance leads to aggravate inflammatory responses. Cd activated the TNF-α/NF-κB pathway and induced inflammatory responses via increasing the expression of HO-1, IL-1β, iNOS, COX2. Heat shock proteins were notably elevated in response to inflammatory reactions. And these effects were inhibited by necrostatin-1 (Nec-1) and N-acetyl-cysteine (NAC). Altogether, these data demonstrated that Cd induced necroptosis and inflammation to aggravate small intestine injury in swine by increasing the excessive accumulation of ROS and imbalanced Th1/Th2, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.126704DOI Listing
January 2022

Exosomal TUBB3 mRNA expression of metastatic castration-resistant prostate cancer patients: Association with patient outcome under abiraterone.

Cancer Med 2021 Sep 28;10(18):6282-6290. Epub 2021 Jul 28.

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Background: To use ddPCR to quantify plasma exosomal class III β-tubulin (βIII-tubulin, TUBB3, encoded by the TUBB3 gene) mRNA expression in metastatic castration-resistant prostate cancer (mCRPC) patients, and study the association of this expression with abiraterone efficacy.

Methods: Blood samples were prospectively collected from 52 mCRPC patients using abiraterone as first-line therapy to measure plasma exosomal TUBB3 mRNA expression value before the initiation of abiraterone. Study endpoints were PSA response rate, PSA-progression-free survival (PSA-PFS), and overall survival (OS, from CRPC to death).

Results: Patients with positive exosomal TUBB3 expression showed shorter PSA-PFS (negative TUBB3 vs. positive TUBB3: 11.0 vs. 7.9 months; p = 0.014). Further analysis demonstrated that patients with strongly positive exosomal TUBB3 (>20 copies/20 µl) was associated with even shorter PSA-PFS (negative TUBB3 vs. positive TUBB3 [<20 copies/20 µl] vs. strongly positive TUBB3 [>20 copies/20 µl]: 11.0 vs. 8.3 vs. 3.6 months, p = 0.005). In multivariate analyzes, TUBB3 (+) (HR: 2.114, p = 0.033) and ECOG score >2 (HR: 3.039, p = 0.006) were independent prognosticators of poor PSA-PFS. PSA response and OS did not present significant differences.

Conclusion: The exosomal TUBB3 mRNA expression level is associated with poor PSA-PFS of abiraterone in mCRPC patients. The detection of exosomal TUBB3 can be valuable in their management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cam4.4168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446399PMC
September 2021
-->