Publications by authors named "Hao Zhong"

116 Publications

Antiaging function of Chinese pond turtle () peptide through activation of the Nrf2/Keap1 signaling pathway and its structure-activity relationship.

Front Nutr 2022 22;9:961922. Epub 2022 Jul 22.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.

Chinese pond turtle is a traditional nourishing food with high nutritional value and bioactivity and has been considered a dietary remedy for prolonging the lifespan since ancient times. However, only limited information about their effects on longevity is available. This study was performed to assess the antioxidant activities and antiaging potential of Chinese pond turtle peptide (CPTP) using model and uncover the possible mechanisms underlying the beneficial effects. CPTP exhibited excellent antioxidant capability with values of 3.31, 1.93, and 9.52 mg/ml for 1,1-diphenyl-2-pycryl-hydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazo-line-6-sulfonic acid) diammonium salt (ABTS), and hydroxyl radical scavenging, respectively. , 0.8% of CPTP significantly extended the mean and median lifespan of female flies by 7.66 and 7.85%, followed by enhanced resistance to oxidative and heat stress. Besides, CPTP remarkably increased the antioxidant enzyme activities and diminished the peroxide product accumulation. Furthermore, CPTP upregulated the relative mRNA expression of antioxidant-related genes, including nuclear factor-erythroid-2-like 2 () and its downstream target genes, while downregulated the expression of Kelch-like ECH-associated protein 1 (). Taken together, CPTP displayed promising potential in both antioxidant and antiaging effects on flies by targeting the Nrf2/Keap1 pathway. Further peptide sequence determination revealed that 89.23% of peptides from the identified sequences in CPTP could exert potential inhibitory effects on Keap1. Among these peptides, ten representative peptide sequences could actively interact with the binding sites of Keap1-Nrf2 interaction through hydrogen bonds, van der Walls, hydrophobic interactions, and electrostatic interactions. Conclusively, CPTP could be utilized as health-promoting bioactive peptide with antioxidant and antiaging capacities.
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http://dx.doi.org/10.3389/fnut.2022.961922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355154PMC
July 2022

Dietary emulsifier glycerol monodecanoate affects the gut microbiota contributing to regulating lipid metabolism, insulin sensitivity and inflammation.

Food Funct 2022 Aug 4. Epub 2022 Aug 4.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Glycerol monodecanoate (GMD) is a medium-chain monoacylglycerol that possesses emulsifying and antibacterial properties. The common emulsifiers carboxymethylcellulose and polysorbate-80 have been reported to cause intestinal microbiota dysbiosis and metabolic disturbances. Glycerol monolaurate (GML), another medium-chain monoacylglycerol, is often used as an emulsifier and could improve metabolism by regulating the gut microbiota. However, research on the effects of GMD on the metabolism and gut microbiota remains scarce. Mice were fed a normal chow diet with or without GMD (150, 800, and 1600 mg kg) for 22 weeks. Metabolism indicators and related genes, gut microbiota, and fecal SCFAs were analyzed. The results demonstrated that GMD significantly improved insulin sensitivity, reduced the serum LPS level, and decreased pro-inflammation cytokines including IL-1β, tumor necrosis factor (TNF), and monocyte chemotactic protein 1 (MCP-1). Additionally, 150 and 1600 mg kg GMD could significantly lower the blood glucose content. 1600 mg kg GMD improved cholesterol metabolism and related gene expression compared to 150 and 800 mg kg GMD. Moreover, 150 and 800 mg kg GMD up-regulated the abundance of and , while 1600 mg kg GMD significantly up-regulated the abundance of . Our findings indicated that different doses of GMD had inconsistent effects on lipid metabolism by differentially altering the gut microbiota composition. Meanwhile, all doses of GMD showed excellent effects on increasing insulin sensitivity and improving inflammation.
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http://dx.doi.org/10.1039/d2fo01689cDOI Listing
August 2022

Consumption of Wheat Peptides Improves Functional Constipation: A Translational Study in Humans and Mice.

Mol Nutr Food Res 2022 Aug 3:e2200313. Epub 2022 Aug 3.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Scope: Wheat peptides (WP) are rich in glutamic acid, glutamine, and other bioactive compounds that may benefit gut function and health. This study aims to evaluate the effects of regular consumption of WP on constipation-induced complications and gut microbiota in humans and mice.

Methods And Results: A randomized trial of 49 functional constipation participants is conducted. The weekly amount of spontaneous bowel movements (SBM) increases by 2.09 per week after WP treatment, and by 0.40 per week among the placebo group (PL). Concomitantly, the secondary outcomes show significant improvements in the quality of life-related to constipation (PAC-QOL), constipation severity, and satisfaction with the intervention. In the animal study, WP effectively alleviates constipation symptoms and affects the secretion of intestinal mobility-related neurotransmitters and gastrointestinal hormones in loperamide-induced constipation mice. Additionally, WP regulates the gene and protein expression levels of water-electrolyte metabolism and intestinal mobility. Furthermore, WP treatment decreases the abundance of several gut microbiota positively correlated to constipation (Turicibacter, Bacteroides_f_Bacteroidaceae, and Streptococcus) in mice.

Conclusion: WP ameliorates constipation in humans and mice, which could be partly explained by improving water-electrolyte metabolism, boosting intestinal motility, and reshaping gut microbiota.
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http://dx.doi.org/10.1002/mnfr.202200313DOI Listing
August 2022

A cold-water polysaccharide-protein complex from Grifola frondosa exhibited antiproliferative activity via mitochondrial apoptotic and Fas/FasL pathways in HepG2 cells.

Int J Biol Macromol 2022 Jul 18;218:1021-1032. Epub 2022 Jul 18.

College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China; Bioactives and Functional Foods Research Center, China National Light Industry, Hangzhou 310014, China; Zhejiang Fangge Pharmaceutical Co., Ltd., Qingyuan 323800, China. Electronic address:

Grifola frondosa (G. frondosa) is widely known for its anti-tumor potential, which has been demonstrated by numerous scientific researches. In this study, two water soluble polysaccharide-protein complexes were extracted from G. frondosa at 4 °C (GFG-4) and 100 °C (GFG-100) and purified. Compared with GFG-100, GFG-4 had a higher protein content and molecular weight. The main monosaccharides of GFG-4 and GFG-100 were rhamnose, glucose, and galactose, with an approximate ratio of 3.00: 1.00: 0.86 and 2.85: 1.00: 0.94, respectively. The Fourier transform infrared spectra indicated that the two polysaccharide-protein complexes displayed characteristic functional groups of polysaccharides and proteins, and mainly contain pyranose ring with α-glycosidic linkage. Atomic force microscope images showed that both GFG-4 and GFG-100 exhibited straight chains, and GFG-4 possessed a relatively abundant fraction of branched chains. Intriguingly, GFG-4 showed a stronger antiproliferative activity against HepG2 cells than GFG-100. The mechanisms were further investigated by quantitative real-time PCR and western blot, it found that GFG-4 inhibited the proliferation of HepG2 cells mainly through the intrinsic activation of mitochondrial pathway and the Fas/FasL-mediated Caspase-8/-3 pathway. Conclusively, G. frondosa cold-water extracted polysaccharide-protein complexes could be used as a functional food for preventing or treating hepatocellular carcinoma.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.098DOI Listing
July 2022

Ultrathin, reflective light-field imaging film realized by self-releasing UV-curable nanoimprinting lithography.

Opt Lett 2022 Jul;47(13):3227-3230

Light-field imaging has emerged as a technology allowing the capture of richer visual information from the world. Ultrathin, reflective light-field imaging film is fabricated by using self-releasing ultraviolet (UV)-curable nanoimprinting lithography. The plenoptic function is built to generate the dense reflective light field in a two-dimension plane in which the occlusion perception can be seamlessly incorporated in the recording process. A self-releasing nanoimprinting technique is developed to realize the imaging film with a thickness of 25 µm and a full field of view (FOV). The results pave the way toward developing high-performance light-field imaging device that can be used as a visual security feature or in virtual/augmented reality and computer vision applications, etc.
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http://dx.doi.org/10.1364/OL.463117DOI Listing
July 2022

Effects of Anthraquinones on Immune Responses and Inflammatory Diseases.

Molecules 2022 Jun 14;27(12). Epub 2022 Jun 14.

State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

The anthraquinones (AQs) and derivatives are widely distributed in nature, including plants, fungi, and insects, with effects of anti-inflammation and anti-oxidation, antibacterial and antiviral, anti-osteoporosis, anti-tumor, etc. Inflammation, including acute and chronic, is a comprehensive response to foreign pathogens under a variety of physiological and pathological processes. AQs could attenuate symptoms and tissue damages through anti-inflammatory or immuno-modulatory effects. The review aims to provide a scientific summary of AQs on immune responses under different pathological conditions, such as digestive diseases, respiratory diseases, central nervous system diseases, etc. It is hoped that the present paper will provide ideas for future studies of the immuno-regulatory effect of AQs and the therapeutic potential for drug development and clinical use of AQs and derivatives.
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http://dx.doi.org/10.3390/molecules27123831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230691PMC
June 2022

Prussian blue analogue/KB-derived Ni/Co/KB composite as a superior adsorption-catalysis separator modification material for Li-S batteries.

J Colloid Interface Sci 2022 Nov 13;625:425-434. Epub 2022 Jun 13.

Guangzhou Key Laboratory of Materials for Energy Conversion and Storage, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, School of Chemistry, South China Normal University, Guangzhou, 510006, PR China. Electronic address:

Lithium‑sulfur batteries (LSBs) are gradually replacing conventional lithium-ion batteries (LIBs), credited to their high theoretical capacity, low cost, and non-toxicity. Nevertheless, the substantial capacity degradation caused by the polysulfide shuttling during charging and discharging has seriously hindered the commercialization of LSBs. Separator modification with functionalized carbon materials has been found to catalyze the breakdown of polysulfides, thereby improving the efficiency of LSBs. Herein, we synthesized Ni/Co-PBAs with KB structures to subsequently derive Ni/Co/KB composites by a carbonization process, which were later used as a modifier layer on the barrier in LSBs in order to effectively alleviate the shuttle problem. The capacity of the Ni/Co/KB composite decorated separator is found to be 1032 mAh/g at 0.5 C with a coulombic efficiency closer to 100%. In the long-term cycling capability evaluation, the initial cycle is approximately 802.9 mAh/g at 1 C, while capacity retention after 400 cycles is also 678.8 mAh/g, with a high-capacity retention rate of 84.5%. The potential of these composites as modifying materials for superior LSBs separators is verified by experimental and theoretical methods.
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http://dx.doi.org/10.1016/j.jcis.2022.06.036DOI Listing
November 2022

Risk factors of chylous ascites and its relationship with long-term prognosis in laparoscopic D3 lymphadenectomy for right colon cancer.

Langenbecks Arch Surg 2022 May 20. Epub 2022 May 20.

Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin Er Road, Shanghai, 200025, China.

Background: Chylous ascites (CA) after laparoscopic D3 lymphadenectomy for right colon cancer is not rare. However, the risk factors for CA have not been fully explored. Few studies have investigated the effect of CA on long-term prognosis.

Methods: The clinical data of patients with right colon cancer who underwent laparoscopic D3 lymphadenectomy in five centers from January 2013 to December 2018 were retrospectively collected. Univariate and multivariate analyses were performed to determine the clinicopathological factors associated with CA. Then, the long-term prognosis of patients with and without CA was compared by propensity score matching and Kaplan-Meier survival analysis.

Results: The incidence of CA was 4.4% (48/1090). Pathological T stage (p = 0.025), dissection along the left side of the superior mesenteric artery (p < 0.001) and the number of retrieved lymph nodes (p < 0.001) were independent risk factors for CA. After propensity score matching, 48 patients in the CA group and 353 patients in the non-CA group were enrolled. Kaplan-Meier survival analysis indicated that CA was not associated with overall survival (p = 0.454) and disease-free survival (p = 0.163). In patients with stage III right colon cancer, there were no significant differences in overall survival (p = 0.501) and disease-free survival (p = 0.254).

Conclusions: Pathological T stage, number of retrieved lymph nodes, and left side dissection along the superior mesenteric artery were independent risk factors for CA after laparoscopic D3 lymphadenectomy. CA does not impair the oncological outcomes of patients.
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http://dx.doi.org/10.1007/s00423-022-02527-3DOI Listing
May 2022

Exploring phylogenetic relationships within the subgenera of Bambusa based on DNA barcodes and morphological characteristics.

Sci Rep 2022 05 16;12(1):8018. Epub 2022 May 16.

Key Laboratory of Resources and Utilization of Bamboo of State Forestry Administration, China National Bamboo Research Center, Hangzhou, 310012, Zhejiang, China.

The genus Bambusa belongs to the subtribe Bambusinae and the subfamily Bambusoideae. The subgenera of Bambusa has not been satisfactorily circumscribed, and this remains a major taxonomic issue. Simultaneously, genera such as Dendrocalamus and Gigantochloa have not been confidently assigned to Bambusa. Here, the phylogenetic relationships among subgenera were investigated using five chloroplast DNA markers (rpl32-trnL, rpl16, matK, rbcL, and trnH-psbA) for a sample of 50 ingroup and 16 outgroup species. A total of 186 key morphological descriptors were studied for the 50 ingroup species. The results indicated that five chloroplast DNA markers were possible to distinguish Bambusa species from other species and divide them into several clusters. Phylogenetic analyses conducted using morphological descriptors and a combined marker (rpl32-trnL+rpl16) revealed three and five distinct lineages, respectively, among the currently recognized Bambusa species. The branching pattern of the dendrogram was not completely consistent with the classical taxonomic classification of Bambusa. In addition, not all varieties and cultivars were clustered with McClure classifications. As the maximum parsimony topology and morphological analyses were inconsistent, some clustering results overlapped. Overall, the results obtained here do not support the current classification of the Bambusa subgenera.
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http://dx.doi.org/10.1038/s41598-022-12094-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110708PMC
May 2022

Correlation Analysis Between Magnetic Resonance Imaging-Based Anatomical Assessment and Behavioral Outcome in a Rat Contusion Model of Chronic Thoracic Spinal Cord Injury.

Front Neurosci 2022 21;16:838786. Epub 2022 Apr 21.

Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China.

Although plenty of evidences from preclinical studies have led to potential treatments for patients with spinal cord injury (SCI), the failure to translate promising preclinical findings into clinical advances has long puzzled researchers. Thus, a more reliable combination of anatomical assessment and behavioral testing is urgently needed to improve the translational worth of preclinical studies. To address this issue, the present study was designed to relate magnetic resonance imaging (MRI)-based anatomical assessment to behavioral outcome in a rat contusion model. Rats underwent contusion with three different heights to simulate various severities of SCI, and their locomotive functions were evaluated by the grid-walking test, Louisville swim scale (LSS), especially catwalk gait analysis system and basic testing, and Basso, Beattie, Bresnahan (BBB) score. The results showed that the lesion area (LA) is a better indicator for damage assessment compared with other parameters in sagittal T2-weighted MRI (T2WI). Although two samples are marked as outliers by the box plot analysis, LA correlated closely with all of the behavioral testing without ceiling effect and floor effect. Moreover, with a moderate severity of SCI in a contusion height of 25 mm, the smaller the LA of the spinal cord measured on sagittal T2WI the better the functional performance, the smaller the cavity region and glial scar, the more spared the myelin, the higher the volatility, and the thicker the bladder wall. We found that LA significantly related with behavior outcomes, which indicated that LA could be a proxy of damage assessment. The combination of sagittal T2WI and four types of behavioral testing can be used as a reliable scheme to evaluate the prognosis for preclinical studies of SCI.
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http://dx.doi.org/10.3389/fnins.2022.838786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069114PMC
April 2022

Guanylate-Binding Protein 1 as a Potential Predictor of Immunotherapy: A Pan-Cancer Analysis.

Front Genet 2022 10;13:820135. Epub 2022 Feb 10.

Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.

Mainstream application of cancer immunotherapy is hampered by the low response rate of most cancer patients. A novel immunotherapeutic target or a biomarker predicting response to immunotherapy needs to be developed. Guanylate-binding protein 1 (GBP1) is an interferon (IFN)-inducible guanosine triphosphatases (GTPases) involving inflammation and infection. However, the immunological effects of GBP1 in pan-cancer patients are still obscure. Using large-scale public data, we delineated the landscape of GBP1 across 33 cancer types. The correlation between GBP1 expression or mutation and immune cell infiltration was estimated by ESTIMATE, TIMER, xCell, and quanTIseq algorithms. GBP1-related genes and proteins were subjected to function enrichment analysis. Clustering analysis explored the relationship between GBP1 expression and anti-tumor immune phenotypes. We assessed the patient's response to immunotherapy using the tumor immune dysfunction and exclusion (TIDE) score and immunophenoscore (IPS). Furthermore, we validated the predictive power of GBP1 expression in four independent immunotherapy cohorts. GBP1 was differentially expressed in tumors and normal tissues in multiple cancer types. Distinct correlations existed between GBP1 expression and prognosis in cancer patients. GBP1 expression and mutation were positively associated with immune cell infiltration. Function enrichment analysis showed that GBP1-related genes were enriched in immune-related pathways. Positive correlations were also observed between GBP1 expression and the expression of immune checkpoints, as well as tumor mutation burden (TMB). Pan-cancer patients with higher GBP1 expression were more inclined to display "hot" anti-tumor immune phenotypes and had lower TIDE scores and higher immunophenoscore, suggesting that these patients had better responses to immunotherapy. Patients with higher GBP1 expression exhibited improved overall survival and clinical benefits in immunotherapy cohorts, including the Gide et al. cohort [area under the curve (AUC): 0.813], the IMvigor210 cohort (AUC: 0.607), the Lauss et al. cohort (AUC: 0.740), and the Kim et al. cohort (AUC: 0.793). This study provides comprehensive insights into the role of GBP1 in a pan-cancer manner. We identify GBP1 expression as a predictive biomarker for immunotherapy, potentially enabling more precise and personalized immunotherapeutic strategies in the future.
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http://dx.doi.org/10.3389/fgene.2022.820135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867058PMC
February 2022

Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice.

Foods 2022 Feb 19;11(4). Epub 2022 Feb 19.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.

The potential of soft-shelled turtle peptides (STP) against fatigue was evaluated. Mice orally supplemented with STP significantly increased the swimming time until tiredness by 35.4-57.1%. Although not statistically significant, STP increased muscle and thymus mass. In addition, the serum lactate, ammonia, blood urea nitrogen content and creatine kinase activity in STP-fed mice were dramatically decreased when compared to the control group. Furthermore, STP supplementation increased the reserves of liver glycogen and muscle glycogen, thus improved the energy metabolism system of mice. STP treatment contributed to increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities as well as a decrease in malondialdehyde (MDA), indicating an improvement in oxidative stress protection. The Western blot (WB) results indicated that the STP supplement effectively altered the expression of oxidative stress-related protein by modulating the NRF2/KEAP1 pathway. In summary, STP affected NRF2/KEAP1 levels in skeletal muscle, leading to antioxidant activity and a slower time to exhaustion during exercise.
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http://dx.doi.org/10.3390/foods11040600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871340PMC
February 2022

Ultrasound-Guided Percutaneous A1 Pulley Release by Acupotomy (Needle-Knife): A Cadaveric Study of Safety and Efficacy.

J Pain Res 2022 9;15:413-422. Epub 2022 Feb 9.

Pain Department, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China.

Purpose: This study was to assess the safety and effectiveness of ultrasound-guided percutaneous A1 pulley release by acupotomy on unembalmed cadavers.

Materials And Methods: Sixty digits (from six cadavers, three male and three female) were split into two groups using stratified randomization. All procedures were completed by a single doctor with rich experience in ultrasound-guided treatment. In the acupotomy group, the A1 pulley was released under ultrasound-guided by a needle-knife; while in the needle group, the A1 pulley was released under ultrasound-guided by a 21-gauge needle. Two groups completed six thumbs and 24 fingers, respectively. Another anatomist, blinded to the two techniques, assessed the safety, including the minimum distance between the incision and the neurovascular; flexor tendon, neurovascular and A2 pulley injury or not. Completeness release of the A1 pulley was recorded as effectiveness.

Results: No neurovascular or A2 pulley injuries were recorded. However, the incision of the thumb in both groups biased to the radial side ( <0.05), while the incision of the finger biased to the ulnar side ( <0.05). No significant flexor tendon injury was found, and only five cases (16.7%) had minor scratches in the acupotomy group; while in the needle group, 15 cases had minor scratches and lacerations occurred in three cases. The flexor tendon injury rate was 60%. Compared with the needle, ultrasound-guided acupotomy release is safer ( <0.05). The ultrasound-guided acupotomy technique was significantly more likely to result in a complete A1 pulley release compared to the needle technique (28 of 30 [93.3%] versus 11 of 30 [36.7%]; <0.05).

Conclusion: Ultrasound-guided percutaneous A1 pulley release by acupotomy is a safe and effective technique. When releasing the thumb by ultrasound-guided, be careful not to bias to the radial side to avoid neurovascular injury, while when releasing a finger, be careful not to bias to the ulnar side.
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http://dx.doi.org/10.2147/JPR.S349869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8842668PMC
February 2022

Deep Learning Algorithm for Automated Detection of Polycystic Ovary Syndrome Using Scleral Images.

Front Endocrinol (Lausanne) 2021 27;12:789878. Epub 2022 Jan 27.

Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.

The high prevalence of polycystic ovary syndrome (PCOS) among reproductive-aged women has attracted more and more attention. As a common disorder that is likely to threaten women's health physically and mentally, the detection of PCOS is a growing public health concern worldwide. In this paper, we proposed an automated deep learning algorithm for the auxiliary detection of PCOS, which explores the potential of scleral changes in PCOS detection. The algorithm was applied to the dataset that contains the full-eye images of 721 Chinese women, among which 388 are PCOS patients. Inputs of the proposed algorithm are scleral images segmented from full-eye images using an improved U-Net, and then a Resnet model was applied to extract deep features from scleral images. Finally, a multi-instance model was developed to achieve classification. Various performance indices such as AUC, classification accuracy, precision, recall, precision, and F1-score were adopted to assess the performance of our algorithm. Results show that our method achieves an average AUC of 0.979 and a classification accuracy of 0.929, which indicates the great potential of deep learning in the detection of PCOS.
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http://dx.doi.org/10.3389/fendo.2021.789878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828568PMC
March 2022

Rapid, Highly Sensitive, and Label-Free Pathogen Assay System Using a Solid-Phase Self-Interference Recombinase Polymerase Amplification Chip and Hyperspectral Interferometry.

Anal Chem 2022 02 2;94(6):2926-2933. Epub 2022 Feb 2.

Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.

Recombinase polymerase amplification (RPA) is a useful pathogen identification method. Several label-free detection methods for RPA amplicons have been developed in recent years. However, these methods still lack sensitivity, specificity, efficiency, or simplicity. In this study, we propose a rapid, highly sensitive, and label-free pathogen assay system based on a solid-phase self-interference RPA chip (SiSA-chip) and hyperspectral interferometry. The SiSA-chips amplify and capture RPA amplicons on the chips, rather than irrelevant amplicons such as primer dimers, and the SiSA-chips are then analysed by hyperspectral interferometry. Optical length increases of SiSA-chips are used to demonstrate RPA detection results, with a limit of detection of 1.90 nm. This assay system can detect as few as six copies of the target 18S rRNA gene of within 20 min, with a good linear relationship between the detection results and the concentration of target genes ( = 0.9903). Single nucleotide polymorphism (SNP) genotyping of the gene of is also possible using the SiSA-chip, with as little as 1% of mutant gene distinguished from wild-type loci (m/wt). This system offers a high-efficiency (20 min), high-sensitivity (6 copies/reaction), high-specificity (1% m/wt), and low-cost (∼1/50 of fluorescence assays for RPA) diagnosis method for pathogen DNA identification. Therefore, this system is promising for fast identification of pathogens to help diagnose infectious diseases, including SNP genotyping.
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http://dx.doi.org/10.1021/acs.analchem.1c04858DOI Listing
February 2022

Effect of perioperative probiotic supplements on postoperative short-term outcomes in gastric cancer patients receiving neoadjuvant chemotherapy: A double-blind, randomized controlled trial.

Nutrition 2022 04 22;96:111574. Epub 2021 Dec 22.

Department of Gastrointestinal Surgery, the Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address:

Objectives: Surgery can significantly improve the prognosis of patients with gastric cancer. However, some patients are at a later stage at diagnosis and need to receive neoadjuvant chemotherapy (NACT). Previous studies have shown that NACT may lead to more postoperative complications. Probiotics have the potential to reduce postoperative complications and infections, but no similar clinical trials have been conducted in patients with gastric cancer receiving NACT. The aim of this study was to investigate the effect of probiotics on postoperative infections and other short-term outcomes in patients with gastric cancer receiving NACT.

Methods: This was a randomized, double-blind, controlled trial. All patients who underwent minimally invasive surgery after NACT were included and randomized into a probiotic group (PG; n = 33) or a control group (CG; n = 33). Postoperative infectious complications, recovery of gastrointestinal function, postoperative hospital stay, medical costs, time to initiate adjuvant chemotherapy, 30-d readmission and mortality, and common laboratory inflammatory indexes were observed.

Results: PG patients had significantly fewer postoperative infections (P = 0.027). Time to first flatus and bowel movement was quicker (P = 0.001 and P < 0.001, respectively) and inflammatory indexes were lower in the PG patients. Postoperative hospital length of stay was shorter in the PG than in the CG (P = 0.001). Due to fewer postoperative infections and faster recovery, adjuvant chemotherapy was initiated earlier in PG patients (P < 0.001).

Conclusions: Perioperative probiotic supplements can reduce postoperative infection, improve short-term clinical outcomes, and reduce the level of common inflammatory indicators in patients with gastric cancer receiving NACT. Patients in the PG started adjuvant chemotherapy earlier than CG patients.
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http://dx.doi.org/10.1016/j.nut.2021.111574DOI Listing
April 2022

Autophagy-inhibiting biomimetic nanodrugs enhance photothermal therapy and boost antitumor immunity.

Biomater Sci 2022 Mar 2;10(5):1267-1280. Epub 2022 Mar 2.

Guangdong Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, P. R. China.

The instinctive protective stress responses of tumor cells hamper low-temperature photothermal therapy (LTPTT), resulting in tumor recurrence and metastasis. The rapid blood clearance and low-efficiency tumor enrichment of nanomedicines also decrease the efficacy of LTPTT. In this study, we fabricated coassembled photothermal agents (indocyanine green, ICG) and autophagy inhibitors (chloroquine, CQ) and red blood cell and cancer cell hybrid membrane (RCm)-camouflaged [email protected] nanoparticles ([email protected] NPs) to enhance tumor LTPTT. The [email protected] NPs exhibited prolonged blood drug circulation and markedly enhanced drug accumulation in tumor tissues. The [email protected] NPs reduced the thermal tolerance of tumor cells to sensitize ICG-mediated LTPTT by inhibiting protective autophagy. The [email protected] NPs exerted strong immunogenic cell death (ICD) after efficient LTPTT to activate antitumor immunity. In addition, [email protected] optimized the therapeutic efficacy by imaging-guided LTPTT, taking advantage of the near-infrared (NIR) fluorescence of ICG. Consequently, the [email protected] NPs effectively inhibited tumors under mild LTPTT, significantly suppressed tumor metastasis and prolonged the survival time of tumor-bearing mice. Furthermore, the [email protected] NPs showed high biosafety and . The [email protected] NPs demonstrated tumor-targeting and imaging-guided autophagy inhibition-sensitized LTPTT using two Food and Drug Administration (FDA)-approved drugs, which have great potential for clinical application.
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http://dx.doi.org/10.1039/d1bm01888dDOI Listing
March 2022

MiR-556-5p modulates migration, invasion, and epithelial-mesenchymal transition in breast cancer cells via targeting PTHrP.

J Mol Histol 2022 Apr 9;53(2):297-308. Epub 2022 Jan 9.

Department of Surgery, Changsha Hospital for Maternal and Child Health Care, No. 416 Chengnan East Road, Yuhua District, Changsha, 410007, Hunan, China.

Breast cancer bone metastases may block normal bone remodeling and promote bone degradation, during which several signaling pathways and small non-coding miRNAs might all play a role. miRNAs and target mRNAs that might be associated with breast cancer bone metastasis were analyzed and selected using bioinformatics analyses based on online data. The 3' untranslated region of key factors associated with breast cancer metastasis were examined for candidate miRNA binding site using Targetscan. The predicted binding was validated. The specific effects of single miRNA and dynamic effects of the miRNA-mRNA axis on breast cancer cell metastasis were investigated. miR-556-5p was downregulated in breast cancer samples according to online datasets and experimental analyses. In breast cancer cells, miR-556-5p overexpression inhibited, whereas miR-556-5p inhibition promoted cancer cell invasion and migration. Among key factors associated with breast cancer bone metastasis, parathyroid hormone related protein (PTHrP) 3'UTR possessed miR-556-5p binding site. Through direct binding, miR-556-5p negatively regulated PTHrP expression. In breast cancer cell lines, miR-556-5p inhibition promoted, whereas PTHrP silencing suppressed cancer cell migration, invasion, and epithelial-mesenchymal transition; the effects of miR-556-5p inhibition were partially reversed by PTHrP silencing. In summary, miR-556-5p targets PTHrP to modulate the cell migration and invasion of breast cancer.
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http://dx.doi.org/10.1007/s10735-021-10056-4DOI Listing
April 2022

ZJUFB2 Prevents High Fat Diet-Induced Insulin Resistance in Association With Modulation of the Gut Microbiota.

Front Nutr 2021 14;8:754222. Epub 2021 Oct 14.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.

ZJUFB2 is a novel probiotic isolate derived from Chinese traditional sourdough that possesses promising probiotics properties. This study aimed to investigate the effects of ZJUFB2 (B2) on insulin sensitivity using mice fed on a high-fat diet (HFD) as well as to explore the involved mechanisms. Purposely, male C57BL/6 mice continuously received an intervention of B2 (~10 CFU/day) for 16 weeks. The results showed that B2 treatment remarkably ameliorated insulin resistance and hyperglycemia in HFD-fed mice. The B2 intervention significantly decreased the hepatic lipid accumulation, serum low-density lipoproteins cholesterol, and lipopolysaccharides, and regulated the bile acids levels as well as liver mRNA expression involved in lipid metabolism. Moreover, the B2 intervention significantly changed the gut microbiota, specifically, showed a lower abundance of obesity-related and inflammation-associated microbes, e.g., and . Furthermore, it exhibited a higher abundance of short-chain fatty acids and bile salt hydrolas-producing bacteria, such as and compared with the HFD group. The findings of this study suggested that B2 is a novel probiotic, effective in preventing insulin resistance by improving the gut microbiota and bile acids.
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http://dx.doi.org/10.3389/fnut.2021.754222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604096PMC
October 2021

Novel Gut Microbiota Patterns Involved in the Attenuation of Dextran Sodium Sulfate-Induced Mouse Colitis Mediated by Glycerol Monolaurate via Inducing Anti-inflammatory Responses.

mBio 2021 10 12;12(5):e0214821. Epub 2021 Oct 12.

College of Biosystems Engineering and Food Science, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Universitygrid.13402.34, Hangzhou, Zhejiang, China.

Inflammatory bowel disease (IBD) is a type of immune-mediated chronic and relapsing inflammatory gastrointestinal symptoms. IBD cannot be completely cured because of the complex pathogenesis. Glycerol monolaurate (GML), naturally found in breast milk and coconut oil, has excellent antimicrobial, anti-inflammatory, and immunoregulatory functions. Here, the protective effect of GML on dextran sodium sulfate (DSS)-induced mouse colitis and the underlying gut microbiota-dependent mechanism were assessed in C57BL/6 mice pretreated or cotreated with GML and in antibiotic-treated mice transplanted with GML-modulated microbiota. Results showed that GML pretreatment has an advantage over GML cotreatment in alleviating weight loss and reducing disease activity index (DAI), colonic histological scores, and proinflammatory responses. Moreover, the amounts of and and fecal propionic acid and butyric acid were elevated only in mice pretreated with GML upon DSS induction. Of note, fecal microbiota transplantation (FMT) from GML-pretreated mice achieved faster and more significant remission of DSS-induced colitis, manifested as reduced DAI, longer colon, decreased histological scores, and enhanced colonic Foxp3 regulatory T cells (Tregs) and ratio of serum anti-inflammatory/proinflammatory cytokines, as well as the reconstruction of microbial communities, including elevated Helicobacter ganmani and decreased pathogenic microbes. In conclusion, GML-mediated enhancement of and fecal short-chain fatty acids (SCFAs) could be responsible for the anticolitis effect. FMT assay confirmed that gut microbiota modulated by GML was more resistant to DSS-induced colitis via elevating beneficial and establishing Treg tolerant phenotype. Importantly, colitis remission induced by GML is associated with novel gut microbiota patterns, even though different microbial contexts were involved. The gut microbiota, which can be highly and dynamically affected by dietary components, is closely related to IBD pathogenesis. Here, we demonstrated that food-grade glycerol monolaurate (GML)-mediated enhancement of and fecal SCFAs could be responsible for the anticolitis effect. FMT assay confirmed that gut microbiota modulated by GML was more resistant to DSS-induced colitis via elevating beneficial and establishing Treg tolerant phenotype. Collectively, colitis remission induced by GML is associated with novel gut microbiota patterns, even though different microbial contexts were involved, which further provided a perspective to identify specific microbial members and those responsible for the anticolitis effect, such as and .
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http://dx.doi.org/10.1128/mBio.02148-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510546PMC
October 2021

Interrelationships and Methods for Improving University Students' Sense of Gain, Sense of Security, and Happiness.

Front Psychol 2021 24;12:729400. Epub 2021 Sep 24.

School of Marxism, Shandong University of Technology, Zibo, China.

The report of the 19th National Congress of the Communist Party of China stressed the necessity to "keep up with people's ever-growing needs for a better life. We will continue to promote social fairness and justice, develop effective social governance, and maintain public order. With this we should see that our people will always have a strong sense of gain, happiness, and sense of security." In this study, 646 university students were surveyed using the Demographic Questionnaire, Sense of Gain Scale, Sense of Security Scale, Orientations to Happiness Scale, and Prosocial Behavior Tendencies Scale to explore the relationships among sense of gain, sense of security and happiness (including meaning, pleasure, and engagement happiness), and to discuss methods for enhancing them on the basis of prosocial behaviors. The results revealed that (1) sense of gain had significant positive correlations with meaning, pleasure, and engagement happiness but a significant negative correlation with sense of security. Sense of security did not have a significant correlation with the three types of happiness. Prosocial behaviors had significant positive correlations with sense of gain and the three types of happiness but shared no significant correlation with sense of security. (2) Sense of gain significantly and positively predicted meaning, pleasure, and engagement happiness, whereas the interaction between sense of security and sense of gain did not yield a significant prediction for the three types of happiness. Prosocial behaviors significantly and positively predicted sense of gain and the three types of happiness. (3) Sense of gain had mediating effects on the relationships between prosocial behaviors and meaning, pleasure, and engagement happiness, whereas sense of security did not have a moderating effect on the relationships between sense of gain and the three types of happiness. Prosocial behaviors directly affect meaning, pleasure, and engagement happiness and can indirectly influence happiness through enhancing a sense of gain. The implementation of prosocial behaviors can not only provide help for others, but also promote the self-satisfaction of the behavior agents and help them get more happiness.
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http://dx.doi.org/10.3389/fpsyg.2021.729400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497965PMC
September 2021

Outdoor Wood Mats-Based Engineering Composite: Influence of Process Parameters on Decay Resistance against Wood-Degrading Fungi   and  .

Polymers (Basel) 2021 Sep 18;13(18). Epub 2021 Sep 18.

Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.

The process parameters significantly influence the preparation and final properties of outdoor wood mats-based engineering composite (OWMEC). During outdoor use, wood composites are susceptible to destruction by rot fungi. Herein, the role of process parameters such as density and resin content on OWMEC resistance to fungal decay was investigated. The poplar OWMEC samples were exposed to white-rot fungus and brown-rot fungus for a period of 12 weeks. The chemical composition, crystallinity, and morphology were evaluated to investigate the effect of process parameters on the chemical composition and microstructure of the decayed OWMEC. With an increase in the density and resin content, the mass loss of the decayed OWMEC decreased. The highest antifungal effect against (12.34% mass loss) and (19.43% mass loss) were observed at a density of 1.15 g/m and resin content of 13%. As results of the chemical composition and microstructure measurements, the resistance of OWMEC against and fungi was improved remarkably by increasing the density and resin content. The results of this study will provide a technical basis to improve the decay resistance of OWMEC in outdoor environments.
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http://dx.doi.org/10.3390/polym13183173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473400PMC
September 2021

The application of machine learning algorithms in predicting the length of stay following femoral neck fracture.

Int J Med Inform 2021 11 13;155:104572. Epub 2021 Sep 13.

International Science and Technology Cooperation Base of Spinal Cord Injury, Tianjin Key Laboratory of Spine and Spinal Cord Injury, Department of Orthopedics, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, China. Electronic address:

Purpose: Femoral neck fracture is a frequent cause of hospitalization, and length of stay is an important marker of hospital cost and quality of care provided. As an extension of traditional statistical methods, machine learning provides the possibility of accurately predicting the length of hospital stay. The aim of this paper is to retrospectively identify predictive factors of the length of hospital stay (LOS) and predict the postoperative LOS by using machine learning algorithms.

Method: Based on the admission and perioperative data of the patients, linear regression was used to analyze the predictive factors of the LOS. Multiple machine learning models were developed, and the performance of different models was compared.

Result: Stepwise linear regression showed that preoperative calcium level (P = 0.017) and preoperative lymphocyte percentage (P = 0.007), in addition to intraoperative bleeding (p = 0.041), glucose and sodium chloride infusion after surgery (P = 0.019), Charlson Comorbidity Index (p = 0.007) and BMI (P = 0.031), were significant predictors of LOS. The best performing model was the principal component regression (PCR) with an optimal MAE (1.525) and a proportion of prediction error within 3 days of 90.91%.

Conclusion: Excessive intravenous glucose and sodium chloride infusion after surgery, preoperative hypocalcemia, preoperative high percentages of lymphocytes, excessive intraoperative bleeding, lower BMI and higher CCI scores were related to prolonged LOS by using linear regression. Machine learning could accurately predict the postoperative LOS. This information allows hospital administrators to plan reasonable resource allocation to fulfill demand, leading to direct care quality improvement and more reasonable use of scarce resources.
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http://dx.doi.org/10.1016/j.ijmedinf.2021.104572DOI Listing
November 2021

A novel immune-related genes signature after bariatric surgery is histologically associated with non-alcoholic fatty liver disease.

Adipocyte 2021 12;10(1):424-434

Department of Gastrointestinal Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.

Increasing evidence shows that immune-related genes (IRGs) play an important role in bariatric surgery (BS). We identified differentially expressed immune-related genes (DEIRGs) of adipose tissue after BS by analysing the two expression profiles of GEO (GSE59034 and GSE29409). Subsequently, enrichment analysis, GSEA and PPI networks were examined to identify the hub IRGs and related pathways. The performance of the signature was evaluated by area under the curve (AUC) of the receiver operating characteristic (ROC). CIBERSORT algorithm was used to evaluate the relative abundance of infiltrated immune cells.42 DEIRGs were found between the GSE59034 and GSE29409 datasets. The AUC of the signature was 0.904 and 0.865 in the GSE58979 and GSE48452, respectively. Interestingly, the signature also showed good performance in diagnosing non-alcoholic fatty liver disease (NAFLD) (AUC was 0.834 and 0.800, respectively). The number of neutrophils, macrophages M2, macrophages M0 and dendritic cells activated decreased significantly. After BS, the infiltration of T cells regulatory, monocytes, mast cells resting and plasma cells in adipose tissue increased. The novel proposed IRGs signature reveals the underlying immune mechanism of BS and is a promising biomarker for distinguishing the severity of NAFLD. This will provide new insights into strategies for treating obesity and NAFLD.
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http://dx.doi.org/10.1080/21623945.2021.1970341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437528PMC
December 2021

Drought stress introduces growth, physiological traits and ecological stoichiometry changes in two contrasting Cunninghamia lanceolata cultivars planted in continuous-plantation soils.

BMC Plant Biol 2021 Aug 18;21(1):379. Epub 2021 Aug 18.

Key Laboratory of National Forestry and Grassland Administration on Bamboo Resources and Utilization, China National Bamboo Research Center, Zhejiang, 310012, Hangzhou, China.

Background: The decrease in Cunninghamia lanceolata (Lamb.) production on continuously planted soil is an essential problem. In this study, two-year-old seedlings of two cultivars (a normal cultivar, NC, and a super cultivar, SC) were grown in two types of soil (not planted (NP) soil; continuously planted (CP) soil) with three watering regimes, and the interactive effects on plant growth and physiological traits were investigated in a greenhouse experiment. The water contents of the soil in the control (CK) (normal water content), medium water content (MWC) and low water content (LWC) treatments reached 75-80 %, 45-50 % and 20-25 % of the field water capacity, respectively.

Results: The results indicated that the CP soil had a negative effect on growth and physiological traits and that the LWC treatment caused even more severe and comprehensive negative effects. In both cultivars, the CP soil significantly decreased the height increment (HI), basal diameter increment (DI), dry matter accumulation (DMA), net photosynthetic rate (Pn), total chlorophyll content (TChl), carotenoid content (Caro) and photosynthetic nitrogen use efficiency (PNUE). Compared to the NP soil, the CP soil also decreased the proline and soluble protein contents, nitrogen use efficiency (NUE) and phosphorus use efficiency (PUE) and increased the nitrogen:phosphorus ratio in roots, stems and leaves. The LWC treatment decreased growth and photosynthesis, changed ecological stoichiometry, induced oxidative stress, promoted water use efficiency and damaged chloroplast ultrastructure. Significant increases in ascorbate peroxidase (APX), peroxidase (POD), soluble protein and proline contents were found in the LWC treatment. Compared with the NC, the SC was more tolerant to the CP soil and water stress, as indicated by the higher levels of DMA, Pn, and WUE. After exposure to the CP soil and watering regimes, the decreases in biomass accumulation and gas exchange were more pronounced.

Conclusions: The combination of drought and CP soil may have detrimental effects on C. lanceolata growth, and low water content enhances the impacts of CP soil stress on C. lanceolata seedlings. The superiority of the SC over the NC is significant in Chinese fir plantation soil. Therefore, continuously planted soil can be utilized to cultivate improved varieties of C. lanceolata and maintain water capacity. This can improve their growth and physiological performance to a certain extent.
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http://dx.doi.org/10.1186/s12870-021-03159-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371764PMC
August 2021

Study on covalent coupling process and flow characteristics of antibody on the surface of immunoassay microfluidic chip.

Prep Biochem Biotechnol 2022 12;52(4):424-432. Epub 2021 Aug 12.

Beijing Key Lab of Precision/Ultra-precision Manufacturing Equipments and Control, Tsinghua University, Beijing, China.

The immune response system of immunoassay microfluidic chips is a dynamic reaction process that continuously sends reactants to the surface of a solid carrier. Signal acquisition results from the heterogeneous immune reactions and reactant transport. Antibody immobilization is the most important part of heterogeneous immune reactions, and reactant transport is reflected in the form of fluid velocity. Here, we reported several surface modification processes on polystyrene substrates that are employed to study the relationship between the antibody immobilization and flow behavior in heterogeneous immune response processes. The antibody was immobilized using covalent grafting. Based on the mechanism of sandwich enzyme linked immunosorbent assay, a fluorescence quantitative detection method was used to evaluate the immune response process. The effects of different surface modification processes on immune response and flow behavior were studied. We identified an optimal flow velocity in the dynamic immune response system in the microfluidic chip. The immune response signal was the strongest when the average flow velocity was approximately 0.2 mm/s in the procalcitonin detection system. Compared with the amino and aldehyde group substrates, the epoxy group substrate has the highest antibody immobilization efficiency; compared with the surface modified by small molecular groups, the introduction of Poly-L-Lysine can increase the amount of antibody immobilization.
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http://dx.doi.org/10.1080/10826068.2021.1958344DOI Listing
April 2022

Perceived Parenting Styles and Mental Health: The Multiple Mediation Effect of Perfectionism and Altruistic Behavior.

Psychol Res Behav Manag 2021 4;14:1157-1170. Epub 2021 Aug 4.

School of Marxism, Shandong University of Technology, Zibo, 255000, People's Republic of China.

Background: According to the ecological systems theory, the microsystems are important during the development process because they have direct effects on immediate and proximal factors that shape human development. The theory identifies the family as a microsystem that has profound influence on development since it is the immediate environment in which individuals live. This study explored the multiple mediation effect of perfectionism and altruistic behavior on the association between perceived parenting styles and mental health.

Methods: In this cross-sectional study, convenience cluster sampling was used, and the purpose was empirically examined by means of an online questionnaire survey. This study adopted the Demographic Questionnaire, short-form Egna Minnen Beträffande Uppfostran, Chinese Frost Multidimensional Perfectionism Scale, Self-Reported Altruism Scale, and General Health Questionnaire to conduct measurements in 525 university students.

Results: The results of the correlation analysis revealed that perceived parenting styles were significantly correlated to perfectionism, altruistic behavior, and mental health. In addition, perfectionism and altruistic behavior were significantly correlated to mental health, while negative perfectionism was not correlated to altruistic behavior. The results of the structural equation model analysis indicated that parental rejection and emotional warmth had direct and significant effects on children's mental health. Positive perfectionism and altruistic behavior not only played partial mediating roles between parental emotional warmth and children's mental health but also exerted a chain multiple mediation effect. Altruistic behavior played a partial mediating role between positive perfectionism and mental health.

Conclusion: Therefore, parents should practice positive parenting styles such as parental emotional warmth toward their children to ensure that positive perfectionism and altruistic behavioral tendency improve mental health.
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http://dx.doi.org/10.2147/PRBM.S318446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349536PMC
August 2021

Postoperative Radiotherapy for Patients With Resectable Stage III-N2 Non-Small Cell Lung Cancer: A Systematic Review and Meta-Analysis.

Front Oncol 2021 15;11:680615. Epub 2021 Jul 15.

Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.

Purpose: For resectable cases of stage III-N2 non-small cell lung cancer (NSCLC), the best treatment after surgery is still uncertain. The effect of postoperative radiotherapy (PORT) is controversial. Thus, we performed this updated meta-analysis to reassess the data of PORT in stage III-N2 NSCLC patients, to figure out whether these patients can benefit from PORT.

Methods: We conducted searches of the published literature in EMBASE, PubMed, and the Cochrane Library for relevant randomized control trials (RCTs) comparing PORT group with the non-PORT group in NSCLC patients at stage III-N2. These studies allowed the prior chemotherapy in the treatment. We extracted the data from these articles and used the hazard ratios (HRs) and their 95% confidence intervals (CIs) as summary statistics for estimating the effect of PORT on overall survival (OS), disease-free survival (DFS), local-regional recurrence-free survival (LRFS).

Result: The analyses of seven randomized controlled trials (1,318 participants) show no benefit of PORT on survival (HR, 0.87; 95% CI, 0.71 to 1.07; p = 0.18) but a significantly different effect of PORT on DFS (HR, 0.83; 95% CI, 0.71 to 0.97; p = 0.02) and LRFS (HR, 0.64; 95% CI, 0.50 to 0.81; p = 0.0003). There is not enough evidence of a difference in the effect on survival by the utility of chemotherapy along with PORT though subgroup analysis of no chemotherapy group, concurrent chemoradiotherapy and sequential chemoradiotherapy group. Even in trials with 3D-CRT radiation technique, the pooled analysis shows no benefit of PORT on survival in patients with stage III-N2 NSCLC (data is not shown).

Conclusion: Our findings illustrate that in the postoperative treatment for patients with stage III-N2 NSCLC, PORT contributes to a significantly increased DFS and LR and may not associate with an improved OS, indicating a cautious selection.
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http://dx.doi.org/10.3389/fonc.2021.680615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320322PMC
July 2021

Impact of glycation on physical properties of composite gluten/zein nanofibrous films fabricated by blending electrospinning.

Food Chem 2022 Jan 13;366:130586. Epub 2021 Jul 13.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China. Electronic address:

In this study, the gluten/zein nanofibrous films were fabricated by blending electrospinning and then glycated with xylose via Maillard reaction. The average fiber diameter of the gluten film decreased from 551 to 343 nm with the increasing ratio of zein, but increased significantly to a range of 717-521 nm after glycation, which induced a higher thermal stability of the nanofibers with an order to disorder transition. The glycated composite films showed the reduced water vapor permeability and improved water stability with a stiffer and more elastic network structure, due to the enhanced intermolecular entanglements and interactions between polymer chains. The results from this work suggested that the composite gluten/zein electrospun films may be glycated via Maillard reaction to obtain desirable physical properties for active food-packaging applications.
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http://dx.doi.org/10.1016/j.foodchem.2021.130586DOI Listing
January 2022

Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine Model.

Stem Cells Int 2021 4;2021:8854479. Epub 2021 Jun 4.

Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, Beijing Key Laboratory (No.BZO381), Beijing 100010, China.

The management of urethral stricture remains a major therapeutic challenge in clinics. Herein, we explored the feasibility of reconstructing a relatively long segment of the urethra by the cell-seeded acellular artery in a canine model. The acellular arterial matrix was obtained from the excised carotid artery of donor dogs. Autologous adipose-derived stem cells (ADSCs) from 6 male dogs were grown and seeded onto the premade acellular arterial matrix. A 3 cm long segment of the urethra was resected in 12 male dogs. Urethroplasty was performed with the acellular arterial matrix seeded with ADSCs in 6 animals and without cells in 6. Serial urethrography was performed at 1 and 3 months postoperatively. Wide urethral calibers without any signs of strictures were confirmed in all 6 animals in the experimental group. In contrast, urethral stricture was demonstrated in 3 animals in the control group. The graft was highly epithelialized and smooth in the experimental group, while graft contracture and scar formation were showed in the control group. Histologic analysis of the cell-seeded arterial matrix at 1 month confirmed the presence of multilayered urothelium and muscle. The levels of tissue formation developed over time with a progressive increase in muscle content. In contrast, extensive fibrosis and sparse smooth muscle were seen in animals treated with matrix without ADSCs. This study provides preclinical evidence that the ADSC-seeded arterial matrix can be used as a tubularized scaffold in the reconstruction of 3 cm long urethral defect in a male canine model. The ADSC-seeded arterial matrix remodels and regenerates normal-appearing urethral tissue layers over time.
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http://dx.doi.org/10.1155/2021/8854479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203373PMC
June 2021
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