Publications by authors named "Hao Zhang"

5,256 Publications

  • Page 1 of 1

Multitask feature learning approach for knowledge graph enhanced recommendations with RippleNet.

PLoS One 2021 14;16(5):e0251162. Epub 2021 May 14.

College of Computer Science and Technology, Jilin University, Changchun, China.

Introducing a knowledge graph into a recommender system as auxiliary information can effectively solve the sparse and cold start problems existing in traditional recommender systems. In recent years, many researchers have performed related work. A recommender system with knowledge graph embedding learning characteristics can be combined with a recommender system of the following three forms: one-by-one learning, joint learning, and alternating learning. For current knowledge graph embedding, a deep learning framework only has one embedding mode, which fails to excavate the potential information from the knowledge graph thoroughly. To solve this problem, this paper proposes the Ripp-MKR model, a multitask feature learning approach for knowledge graph enhanced recommendations with RippleNet, which combines joint learning and alternating learning of knowledge graphs and recommender systems. Ripp-MKR is a deep end-to-end framework that utilizes a knowledge graph embedding task to assist recommendation tasks. Similar to the MKR model, in the Ripp-MKR model, two tasks are associated with cross and compress units, which automatically share latent features and learn the high-order interactions among items in recommender systems and entities in the knowledge graph. Additionally, the model borrows ideas from RippleNet and combines the knowledge graph with the historical interaction record of a user's historically clicked items to represent the user's characteristics. Through extensive experiments on real-world datasets, we demonstrate that Ripp-MKR achieves substantial gains over state-of-the-art baselines in movie, book, and music recommendations.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251162PLOS
May 2021

The autistic-like behaviors development during weaning and sexual maturation in VPA-induced autistic-like rats is accompanied by gut microbiota dysbiosis.

PeerJ 2021 3;9:e11103. Epub 2021 May 3.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.

Researches on gut microbiota in autism have mostly focused on children, but the dynamic changes of gut microbiota from weaning to adulthood were still not clear because of the difficulty of diagnosing autism. In this study, autistic-like rats indued by valproate (VPA) were tracked from weaning (end of breastfeeding; four weeks old) to sexual maturation (food; eight weeks old). Autistic-like rats were found to show obvious developmental disorders. During weaning, autistic-like rats only exhibited obvious repetitive stereotyped behaviors, but the autistic-like behaviors were fully apparent upon sexual maturation. Significant differences were observed between the gut microbiota of autistic-like and healthy rats across both age groups. The correlation analysis results revealed that the correlation between behaviors and some microbiota, especially , did not vary with age or diet. The total amount of short-chain fatty acids (SCFAs) decreased, butyric acid metabolism decreased, and propionic acid metabolism increased in the feces of autistic-like rats. The correlation between autistic-like behaviors and the butyric acid and propionic acid levels did not vary with diet or age. Inositol phosphate metabolism, amino acid metabolism, and lipopolysaccharide biosynthesis were significantly associated with autistic-like behaviors. Our results showed that although the microbiota and SCFAs related to autism were affected by age and diet, some remained consistent irrespective of age and diet, and they could be considered two of the factors related to autistic-like behaviors development.
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http://dx.doi.org/10.7717/peerj.11103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101471PMC
May 2021

Phase hologram optimization with bandwidth constraint strategy for speckle-free optical reconstruction.

Opt Express 2021 Apr;29(8):11645-11663

An iterative method with bandwidth constraint strategy is proposed to design phase holograms for high-quality speckle-free optical reconstruction. The bandwidth properties of the reconstructed field are analyzed theoretically based on the sampling theory, which helps in properly allocating the sampling resources for efficiently describing the speckles and artifacts in the reconstructed field. Iterative calculation with bandwidth constraint strategy of the reconstructed field and quadratic initial phase can optimize the phase hologram without stagnation problem, which provides effective controls of the reconstructed intensity fluctuations and helps to suppress the speckles and artifacts. Numerical and optical experiments have been performed to validate the proposed method can achieve excellent image fidelity.
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http://dx.doi.org/10.1364/OE.422115DOI Listing
April 2021

Improving spatial precision and field-of-view in wavelength-tagged single-particle tracking using spectroscopic single-molecule localization microscopy.

Appl Opt 2021 May;60(13):3647-3658

Spectroscopic single-molecule localization microscopy (sSMLM) generates super-resolution images of single molecules while simultaneously capturing the spectra of their fluorescence emissions. However, sSMLM splits photons from single-molecule emissions into a spatial channel and a spectral channel, reducing both channels' precisions. It is also challenging in transmission grating-based sSMLM to achieve a large field-of-view (FOV) and avoid overlap between the spatial and spectral channels. The challenge in FOV has further significance in single-molecule tracking applications. In this work, we analyzed the correlation between the spatial and spectral channels in sSMLM to improve its spatial precision, and we developed a split-mirror assembly to enlarge its FOV. We demonstrate the benefits of these improvements by tracking quantum dots. We also show that we can reduce particle-identification ambiguity by tagging each particle with its unique spectral characteristics.
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http://dx.doi.org/10.1364/AO.415275DOI Listing
May 2021

Targeting the Gut Microbiota for Remediating Obesity and Related Metabolic Disorders.

J Nutr 2021 May 12. Epub 2021 May 12.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.

The rate of obesity is rapidly increasing and has become a health and economic burden worldwide. As recent studies have revealed that the gut microbiota is closely linked to obesity, researchers have used various approaches to modulate the gut microbiota to treat the condition. Dietary composition and energy intake strongly affect the composition and function of the gut microbiota. Intestinal microbial changes alter the composition of bile acids and fatty acids and regulate bacterial lipopolysaccharide production, all of which influence energy metabolism and immunity. Evidence also suggests that remodeling the gut microbiota through intake of probiotics, prebiotics, fermented foods, and dietary plants, as well as by fecal microbiota transplantation, are feasible methods to remediate obesity.
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http://dx.doi.org/10.1093/jn/nxab103DOI Listing
May 2021

Facile Exfoliation for High-Quality Molybdenum Disulfide Nanoflakes and Relevant Field-Effect Transistors Developed With Thermal Treatment.

Front Chem 2021 26;9:650901. Epub 2021 Apr 26.

College of Science, Shanghai Institute of Technology, Shanghai, China.

Molybdenum disulfide (MoS), a typical member of the transition metal dichalcogenides (TMDs) group, is known for its excellent electronic performance and is considered a candidate next-generation semiconductor. The preparation of MoS nanoflakes for use as the core of semiconducting devices depends on mechanical exfoliation, but its quality has not yet been optimized. In this paper, a novel exfoliation method of achieving MoS nanoflakes is proposed. We find that the size and yield of the exfoliated flakes are improved after thermal treatment for 2 h at a temperature of 110°C followed by precooling for 10 min in ambient air. The new method has the advantage of a 152-fold larger size of obtained MoS flakes than traditional mechanical exfoliation. This phenomenon may be attributable to the differences in van Der Waals force and the increase in surface free energy at the interface induced by thermal treatment. In addition, a field-effect transistor (FET) was fabricated on the basis of multilayer MoS prepared according to a new process, and the device exhibited a typical depleted-FET performance, with an on/off ratio of ~10 and a field-effect mobility of 24.26 cm/Vs in the saturated region when is 10 V, which is generally consistent with the values for devices reported previously. This implies that the new process may have potential for the standard preparation of MoS and even other 2D materials as well.
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http://dx.doi.org/10.3389/fchem.2021.650901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109793PMC
April 2021

The extracellular-regulated protein kinase 5 (ERK5) enhances metastatic burden in triple-negative breast cancer through focal adhesion protein kinase (FAK)-mediated regulation of cell adhesion.

Oncogene 2021 May 12. Epub 2021 May 12.

Division of Cancer Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.

There is overwhelming clinical evidence that the extracellular-regulated protein kinase 5 (ERK5) is significantly dysregulated in human breast cancer. However, there is no definite understanding of the requirement of ERK5 in tumor growth and metastasis due to very limited characterization of the pathway in disease models. In this study, we report that a high level of ERK5 is a predictive marker of metastatic breast cancer. Mechanistically, our in vitro data revealed that ERK5 was critical for maintaining the invasive capability of triple-negative breast cancer (TNBC) cells through focal adhesion protein kinase (FAK) activation. Specifically, we found that phosphorylation of FAK at Tyr397 was controlled by a kinase-independent function of ERK5. Accordingly, silencing ERK5 in mammary tumor grafts impaired FAK phosphorylation at Tyr397 and suppressed TNBC cell metastasis to the lung without preventing tumor growth. Collectively, these results establish a functional relationship between ERK5 and FAK signaling in promoting malignancy. Thus, targeting the oncogenic ERK5-FAK axis represents a promising therapeutic strategy for breast cancer exhibiting aggressive clinical behavior.
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http://dx.doi.org/10.1038/s41388-021-01798-2DOI Listing
May 2021

Aberrant expression of miR-148a-3p in Alzheimer's disease and its protective role against amyloid-β induced neurotoxicity.

Neurosci Lett 2021 May 9:135953. Epub 2021 May 9.

Department of Neurology, Shanxian Central Hospital, Heze 274300, Shandong, China. Electronic address:

Objective: The current study investigated the expression change and clinical value of miR-148a-3p in AD patients, and further examined the role of miR-148a-3p in Aβ-induced neurotoxicity in SH-SY5Y cells.

Material And Methods: qRT-PCR was used for the measurement of miR-148a-3p expression levels. ROC curve was established to calculate the diagnostic value of serum miR-148a-3p for AD. CCK-8 and flow cytometry assay was applied for the detection of cell viability and apoptosis. Additionally, the luciferase reporter assay was performed to confirm the target relationship between ROCK1 and miR-148a-3p.

Results: Serum miR-148a-3p was downregulated in AD patients compared with that in healthy controls, and was positively associated with the MMSE score in AD patients. Serum miR-148a-3p had the potential to distinguish AD patients from healthy controls, and the diagnostic sensitivity and specificity were respectively 85.5% and 87.0% at a cutoff value of 0.827. MiR-148a-3p attenuated Aβ25-35 induced neurotoxicity in SH-SY5Y cells, and ROCK1 was the target gene.

Conclusion: Serum miR-148a-3p is correlated with MMSE score in AD patients, and it might be helpful for the AD diagnosis. Overexpression of miR-148a-3p attenuated Aβ induced neurotoxicity in AD by targeting ROCK1.
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http://dx.doi.org/10.1016/j.neulet.2021.135953DOI Listing
May 2021

Precise Identification of the Dimethyl Sulfoxide Triggered Tricarbonyldichlororuthenium(II) Dimer for Releasing CO.

J Phys Chem Lett 2021 May 12:4658-4665. Epub 2021 May 12.

Beijing National Laboratory for Condensed Matter Physics and CAS Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

Low concentrations of carbon monoxide (CO) can play vital roles in pharmacological and physiological functions in the human body. The transition-metal carbonyl complexes of the tricarbonyldichlororuthenium(II) dimer [Ru(CO)Cl (CORM-2)] were proposed as CO-releasing molecules (CORMs) to improve the delivery efficiency of CO for therapeutic effects. The accurate identification of final products for CORMs in solution and the detailed mechanisms of the release of CO were the essential prerequisite for its effective physiological application, which have been deficient. In this study, utilizing the cutting-edge two-dimensional (2D) IR spectroscopy, with the intrinsic vibrational modes and the coupling information on dynamics of intramolecular vibrational energy redistribution (IVR), the final products of A, B, C, and E are accurately identified when CORM-2 is dissolved in dimethyl sulfoxide (DMSO). Furthermore, with the clues on intermolecular interaction and chemical exchange dynamics between different products, the transformations between different products are also directly characterized for the first time. These findings challenge the results from the classic 1D spectroscopic pattern, and they evidently demonstrated that the release of CO from CORM-2 in DMSO was slow and complicated with multiple reaction pathways. Combining with DFT simulations, the detailed mechanisms of release of CO for CORM-2 dissolved in DMSO are schematically proposed, which can significantly contribute to its drug optimization and pharmacological as well as physiological applications.
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http://dx.doi.org/10.1021/acs.jpclett.1c00905DOI Listing
May 2021

Study on volume change and scanning electron microscopy observation of silica-filled solution polymerized styrene-butadiene rubber/cis-1,4-polybutadiene rubber blends upon stretching.

Microsc Res Tech 2021 May 12. Epub 2021 May 12.

Department of R&D, EVE Rubber Institute Co., Ltd., Qingdao, China.

Solution polymerized styrene-butadiene rubber/cis-1,4-polybutadiene rubber (SBR/BR) blends filled with silica is widely used system in tread compounds for passenger tires. The application of silane coupling agents greatly improves the interaction between polymer and filler through chemical bonds. By testing a series of SBR/BR blends filled with different specific surface area and content of silica, we measured the volume change data of the compounds upon stretching and plotted the corresponding ΔV/V-ε curves. In order to normalize for stress differences, ΔV/(Vσ)-ε curves were also plotted using stress-strain data. Scanning electron microscopy (SEM) was used to observe the microstructures in the vulcanizate in situ when stretched. The dispersion, agglomeration, and vacuole formation process can be visualized clearly in the SEM images.
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http://dx.doi.org/10.1002/jemt.23814DOI Listing
May 2021

FJSYC4-1 and FGSZY33L6 alleviate metabolic syndrome gut microbiota regulation.

Food Funct 2021 May;12(9):3919-3930

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China. and School of Food Science and Technology, Jiangnan University, Wuxi, China and National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, China and Beijing Innovation Center of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, China.

Metabolic syndrome, which includes a series of metabolic disorders such as hyperglycemia, hyperlipidemia, insulin resistance and obesity, has become a catastrophic disease worldwide. Accordingly, probiotic intervention is a new strategy to alleviate metabolic syndrome, which can adjust the gut microbiota to a certain extent. The aim of the current work was to explore the alleviation of metabolic syndrome by Lactobacillus reuteri and L. rhamnosus. Two L. reuteri and two L. rhamnosus strains were administered to mice with a high-fat diet for 12 weeks. All Lactobacillus strains tested significantly slowed weight gain in the mice. Among four strains, L. reuteri FGSZY33L6 and L. rhamnosus FJSYC4-1 showed the strongest ability to relieve blood glucose disorders, blood lipid disorders, tissue damage, and particularly gut microbiota disorders. Thus, our findings indicate that these strains can regulate the gut microbiota and produce short-chain fatty acids (SCFAs), which can induce satiety hormones, inhibit food intake and increase satiety, and thus improve metabolic syndrome.
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http://dx.doi.org/10.1039/d0fo02879gDOI Listing
May 2021

Effect of alfalfa (Medicago sativa L.) saponins on meat color and myoglobin reduction status in the longissimus thoracis muscle of growing lambs.

Anim Sci J 2021 Jan;92(1):e13556

State Key Laboratory of Animal Nutrition, Collage of Animal Science and Technology, China Agricultural University, Beijing, China.

The effect of alfalfa saponins (AS) supplementation on the meat quality especially the color for growing lamb was investigated. Fifty Hu male lambs with body weights (BW, 19.21 ± 0.45 kg) were divided into five groups and supplemented AS with 0, 500, 1,000, 2,000, and 4,000 mg/kg of dietary dry matter intake. After 90 days, all lambs were slaughtered. The longissimus thoracis muscle in lamb displayed significant changes in the content of intramuscular fat, especially n-3 polyunsaturated fatty acids, and drip loss within AS treatment (p < .05) between control and treatments groups. Redness (a*) significantly improved in both 0-day and 7-day storage with the AS supplementation coupled with the percentage of met-myoglobin reduction (p < .05). The redness (a*) change may result from improved met-myoglobin reducing activity, antioxidant enzymes, lactate dehydrogenase, and succinate dehydrogenase (p < .05) by AS supplementation in muscle. These enzymes may help to protect mitochondria function and reduce met-myoglobin, which bring a bright and red meat color.
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http://dx.doi.org/10.1111/asj.13556DOI Listing
January 2021

Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype .

Front Microbiol 2021 22;12:674068. Epub 2021 Apr 22.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

() is an important nosocomial and community acquired opportunistic pathogen which causes various infections. The emergence of multi-drug resistant (MDR) and carbapenem-resistant hypervirulent (CR-hvKP) has brought more severe challenge to the treatment of infection. In this study, a novel bacteriophage that specifically infects was isolated and named as vB_KpnM_P-KP2 (abbreviated as P-KP2). The biological characteristics of P-KP2 and the bioinformatics of its genome were analyzed, and then the therapeutic effect of P-KP2 was tested by animal experiments. P-KP2 presents high lysis efficiency . The genome of P-KP2 shows homology with nine phages which belong to "" family and its genome comprises 172,138 bp and 264 ORFs. Besides, P-KP2 was comparable to gentamicin in the treatment of lethal pneumonia caused by W-KP2 (K47 serotype). Furthermore, the combined treatment of P-KP2 and gentamicin completely rescued the infected mice. Therefore, this study not only introduces a new member to the phage therapeutic library, but also serves as a reference for other phage-antibiotic combinations to combat MDR pathogens.
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http://dx.doi.org/10.3389/fmicb.2021.674068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100603PMC
April 2021

Heavy ozone pollution episodes in urban Beijing during the early summertime from 2014 to 2017: Implications for control strategy.

Environ Pollut 2021 Apr 27;285:117162. Epub 2021 Apr 27.

Environment Research Institute, Shandong University, Qingdao, 266237, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.

Ground-level ozone (O) has become the principal air pollutant in Beijing during recent summers. In this context, an investigation of ambient concentrations and variation characteristics of O and its precursors in May and June from 2014 to 2017 in a typical urban area of Beijing was carried out, and the formation sensitivity and different causes of heavy O pollution (HOP, daily maximum 8-h O (MDA8h O)>124 ppbv) were analyzed. The results showed that the monthly assessment values of the O concentrations (the 90 percentile MDA8h O within one month) were highest in May or June from 2014 to 2017, and the values presented an overall increasing trend. During this period, the number of O pollution days (MDA8h O > 75 ppbv) also showed an increasing trend. During the HOP episodes, the concentrations of volatile organic compounds (VOCs), nitrogen oxides (NO), and carbon monoxide (CO) were higher than their respective mean values in May and June, and the meteorological conditions were more conducive to atmospheric photochemical reactions. The HOP episodes were mainly caused by local photochemical formation. From 2014 to 2017, O formation during the HOP episodes shifted from VOC and NO mixed-limited to VOC-limited conditions, and O formation was most sensitive to anthropogenic VOCs. Six categories of VOC sources were identified, among which vehicular exhaust contributed the most to anthropogenic VOCs. The VOC concentrations and OFPs of anthropogenic sources have decreased significantly in recent years, indicating that VOC control measures have been effective in Beijing. Nevertheless, NO concentrations did not show an evident decreasing trend in the same period. Therefore, more attention should be devoted to O pollution control in May and June; control measure adjustments are needed according to the changes in O precursors, and coordinated control of VOCs and NO should be strengthened in long-term planning.
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http://dx.doi.org/10.1016/j.envpol.2021.117162DOI Listing
April 2021

Lycopene prevents DEHP-induced hepatic oxidative stress damage by crosstalk between AHR-Nrf2 pathway.

Environ Pollut 2021 Apr 7;285:117080. Epub 2021 Apr 7.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, PR China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address:

Di (2-ethylhexyl) phthalate (DEHP) is a widespread plasticizer that persists in the environment and can significantly contribute to serious health hazards of liver especially oxidative stress injury. Lycopene (LYC) as a carotenoid has recently gained widespread attention because of antioxidant activity. However, the potential mechanism of DEHP-induced hepatotoxicity and antagonism effect of LYC on it are still unclear. To explore the underlying mechanisms of this hypothesis, the mice were given by gavage with LYC (5 mg/kg) and DEHP (500 or 1000 mg/kg). The data suggested that DEHP caused liver enlargement, reduction of antioxidant activity markers, increase of oxidative stress indicators and disorder of cytochrome P450 enzymes system (CYP450s) homeostasis. DEHP-induced reactive oxygen species (ROS) activated the NF-E2-relatedfactor2 (Nrf2) and nuclear xenobiotic receptors (NXRs) system including Aryl hydrocarbon receptor (AHR), Pregnane X receptor (PXR) and Constitutive androstane receptor (CAR). Interestingly, these disorders and injuries were prevented after LYC treatment. Taken together, DEHP administration resulted in hepatotoxicity including oxidative stress injury and disordered CYP450 system, but these alterations might be ameliorated by LYC via crosstalk between AHR-Nrf2 pathway.
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http://dx.doi.org/10.1016/j.envpol.2021.117080DOI Listing
April 2021

Epidemiological trends of maternal hypertensive disorders of pregnancy at the global, regional, and national levels: a population-based study.

BMC Pregnancy Childbirth 2021 May 8;21(1):364. Epub 2021 May 8.

Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, 710061, Xi'an, Shaanxi, China.

Background: Relevant studies focusing on epidemiological of profiles hypertensive disorders of pregnancy from global data that report the cause-specific prevalence and trends of hypertensive disorders of pregnancy at global, regional and national levels from 1990 to 2019 by age and sociodemographic index are still limited.

Methods: For hypertensive disorders of pregnancy, point prevalence, annual incidence, and years lived with disability numbers and age standardized rates per 100,000 population were compared at regional and national levels by age and sociodemographic index using data from the global Burden of Disease 2019 Study, covering populations from 204 countries and territories. Estimates are reported with uncertainty intervals to exhibit the changing trends during a specific period.

Results: The incidence of hypertensive disorders of pregnancy increased from 16.30 million to 18.08 million globally, with a total increase of 10.92 % from 1990 to 2019. The age-standardized incidence rate decreased, with an estimated annual percent change of -0.68 (95 % confidence interval [CI] -0.49 to -0.86). The number of deaths due to hypertensive disorders of pregnancy was approximately 27.83 thousand in 2019, representing a 30.05 % decrease from 1990. Based on the incidence and prevalence, the number of deaths and years lived with disability were highest in the group aged 25-29 years, followed by the groups aged 30-34 and 20-24 years, while the lowest estimated incidence rate was observed in the group aged 25-29 years and higher incidence rates were observed in the youngest and oldest groups. Positive associations between incidence rates and the sociodemographic index and human development index were found for all countries and regions in 2019. Age-standardized incidence rates were higher in countries/regions with lower sociodemographic indices and human development indices.

Conclusions: Our study provides a comprehensive overview of the global burden of hypertensive disorders of pregnancy. The death and incidence rates are decreasing in most countries and all regions except for those with low sociodemographic and human development indexes. This difference is mainly due to the increasing attention to prenatal examinations and health education. Further investigations should focus on forecasting the global disease burden of specific hypertensive disorders of pregnancy and modifiable risk factors.
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http://dx.doi.org/10.1186/s12884-021-03809-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106862PMC
May 2021

Enantiomeric profiling of a chiral benzothiazole necroptosis inhibitor.

Bioorg Med Chem Lett 2021 May 6;43:128084. Epub 2021 May 6.

School of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Yinchuan 750004, China; School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China. Electronic address:

Necroptosis is a form of programmed cell death that contributes to the pathophysiology of multiple diseases. Development of small-molecule anti-necroptosis agents has great promising clinical therapeutic relevance. The benzothiazole compounds were discovered by our group from an in-house fluorine-containing compound library as potent necroptosis inhibitors. Herein, a chiral dimethylcyclopropyl benzothiazole necroptosis inhibitor was developed and the enantiomeric profiling resulted that the (S) form was generally more potent than the (R) counterpart in 2 ~ 4-fold toward cell necroptosis, receptor-interacting protein (RIP) kinases 1 and 3. The chiral compounds could significantly inhibit the expression of the phosphorylation of RIPK1, RIPK3 and MLKL in necroptotic cells. The molecular modelling studies predicted the binding modes of the enantiomers with RIP and explained their activity differences, guiding further rational design of the chiral necroptosis inhibitors.
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http://dx.doi.org/10.1016/j.bmcl.2021.128084DOI Listing
May 2021

The Predictive Value of Monocytes in Immune Microenvironment and Prognosis of Glioma Patients Based on Machine Learning.

Front Immunol 2021 16;12:656541. Epub 2021 Apr 16.

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.

Gliomas are primary malignant brain tumors. Monocytes have been proved to actively participate in tumor growth. Weighted gene co-expression network analysis was used to identify meaningful monocyte-related genes for clustering. Neural network and SVM were applied for validating clustering results. Somatic mutation and copy number variation were used for defining the features of identified clusters. Differentially expressed genes (DEGs) between the stratified groups after performing elastic regression and principal component analyses were used for the construction of risk scores. Monocytes were associated with glioma patients' survival and exhibited high predictive value. The prognostic value of risk score in glioma was validated by the abundant expression of immune checkpoint and metabolic profile. Additionally, high risk score was positively associated with the expression of immunogenic and antigen presenting factors, which indicated high immune infiltration. A prognostic model based on risk score demonstrated high accuracy rate of receiver operating characteristic curves. Compared with previous studies, our research dissected functional roles of monocytes from large-scale analysis. Findings of our analyses strongly support an immune modulatory and prognostic role of monocytes in glioma progression. Notably, monocyte could be an effective predictor for therapy responses of glioma patients.
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http://dx.doi.org/10.3389/fimmu.2021.656541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8095378PMC
April 2021

π-Extended Nonfullerene Acceptors for Efficient Organic Solar Cells with a High Open-Circuit Voltage of 0.94 V and a Low Energy Loss of 0.49 eV.

ACS Appl Mater Interfaces 2021 May 6. Epub 2021 May 6.

Chinese Academy of Sciences (CAS) Key Laboratory of Nano System and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.

A combination of high open-circuit voltage () and short-circuit current density () typically creates effective organic solar cells (OSCs). Y5, a member of the Y-series acceptors, can achieve high of 0.94 V with PM6 but low of 12.8 mA cm. To maintain the high while increasing the of devices, we developed a new nonfullerene acceptor, namely, BTP-C2C4-N, by extending the conjugation of a Y5 molecule with a naphthalene-based end acceptor. In comparison with Y5-based devices, PM6:BTP-C2C4-N-based devices exhibited significantly higher of 18.2 mA cm followed by a high . To further increase the photovoltaic properties of BTP-C2C4-N analogues, BTP-C4C6-N and BTP-C6C8-N molecules with better processability and film morphology are obtained by adjusting the alkyl branched chain length. The optimized OSCs based on BTP-C4C6-N with a moderate alkyl branched chain length exhibited the best PCE of 12.4% with a high of 0.94 V and of 20.7 mA cm. Notably, the devices achieved a low energy loss of 0.49 eV (0.51 eV for Y5 system) accompanied by a small nonradiative energy loss. The results indicate that nonfullerene acceptors with extended terminal motifs and optimized branched chain lengths can effectively enhance the performance of OSCs and reduce energy loss.
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http://dx.doi.org/10.1021/acsami.1c04273DOI Listing
May 2021

Egg consumption associated with all-cause mortality in rural China: a 14-year follow-up study.

Eur J Public Health 2021 May 5. Epub 2021 May 5.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.

Background: Dietary recommendations regarding egg intake remain controversial topic for public health. We hypothesized that there was a positive association between egg consumption and all-cause mortality.

Methods: To test this hypothesis, we enrolled 9885 adults from a community-based cohort in Anhui Province, China during 2003-05. Egg consumption was assessed by food questionnaire. Stratified analyses were performed for age, sex, body mass index (BMI), blood pressure, smoking, drinking and laboratory tests.

Results: After an average follow-up of 14.1 years, 9444 participants were included for analysis. A total of 814 deaths were recorded. Participants' BMI and lipid profile had no significantly difference between three egg consumption groups. BMI was 21.6±2.7 of the whole population, especially BMI>24 was only 17.3%. A bivariate association of egg consumption >6/week with increased all-cause mortality was observed compared with ≤6/week (RR: 1.35, 95% CI: 1.05, 1.73, P = 0.018). A significant interaction was observed for BMI ≥ 21.2 kg/m2 vs. BMI<21.2 kg/m2 (P for interaction: 0.001). No other significant interactions were found.

Conclusions: In this study, consuming >6 eggs/week increased risk of all-cause mortality, even among lean participants, especially who with BMI ≥ 21.2 kg/m2. Eggs are an easily accessible and constitute an affordable food source in underdeveloped regions. Consuming <6 eggs/week may be the most suitable intake mode.
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http://dx.doi.org/10.1093/eurpub/ckaa250DOI Listing
May 2021

Copper Phosphide Nanoparticles Used for Combined Photothermal and Photodynamic Tumor Therapy.

ACS Biomater Sci Eng 2021 May 5. Epub 2021 May 5.

Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

Copper-based nanomaterials are widely used in near-infrared (NIR) light-mediated deep tumor treatment because of their abundant photothermal and photodynamic properties. However, copper phosphide (CuP) nanoparticles (NPs) are rarely investigated. Herein, CuP NPs were prepared to strengthen their local surface plasmon resonance absorption in the NIR region, exhibiting promising photothermal and photodynamic properties. After surface modification by polyethylene glycol, the formed CuP NPs showed negligible influence on the viability of 4T1 cells, presenting remarkable biocompatibility. However, with 808 nm irradiation, CuP NPs could induce HSP70 and HO-1 protein expression and enhance intracellular reactive oxygen species levels, leading to dramatic cell death. In 4T1 tumor-bearing mice, an intravenous injection of biocompatible CuP NP could lead to remarkable aggregation in the tumor region and significantly inhibit tumor growth under 808 nm laser irradiation, presenting great potential for tumor therapy.
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http://dx.doi.org/10.1021/acsbiomaterials.1c00189DOI Listing
May 2021

Achieving full-color emission of Cu nanocluster self-assembly nanosheets by the virtue of halogen effects.

Soft Matter 2021 May;17(17):4550-4558

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China. and Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou, 450001, P. R. China.

Fluorescent Cu nanoclusters (NCs) have shown potential in lighting and display, because Cu is cheap and easily available. Despite recent successes in improving the emission intensity of Cu NCs on the basis of aggregation-induced emission enhancement and self-assembly-induced emission enhancement, the difficulty in tuning the emission color sheds the doubt for achieving high-performance white light-emitting diodes (WLEDs). In this work, halogen effects are utilized to tune the emission color of Cu nanocluster self-assembly nanosheets (NSASs). By altering the adsorbed halogens from Cl, Br to I, the emission peak of Cu NSASs is tunable from 495 to 674 nm. In this context, halogen atoms are capable of improving the charge transfer and molecular spin coupling of Cu NCs, and thereby narrow the S0T1 gap and facilitate the intersystem crossing of excitons from a singlet to triplet state. As a result, emission spectra redshift and the population of the exiton recombination via the triplet state pathway is increased, which leads to the improvement of the photoluminescence quantum yield (PLQY). By simply introducing and/or mixing different types of cuprous halides, Cu nanocluster co-assembly nanosheets (NCASs) with full-color emission are obtained. The as-prepared Cu NSASs and NCASs are further employed to fabricate monochrome and white LEDs.
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http://dx.doi.org/10.1039/d1sm00061fDOI Listing
May 2021

Macrocyclic Peptides that Selectively Inhibit the Proteasome.

J Med Chem 2021 May 5;64(9):6262-6272. Epub 2021 May 5.

Department of Microbiology & Immunology, Weill Cornell Medicine, 1300 York Avenue, New York, New York 10065, United States.

Treatment of tuberculosis (TB) currently takes at least 6 months. Latent (Mtb) is phenotypically tolerant to most anti-TB drugs. A key hypothesis is that drugs that kill nonreplicating (NR) Mtb may shorten treatment when used in combination with conventional drugs. The Mtb proteasome (Mtb20S) could be such a target because its pharmacological inhibition kills NR Mtb and its genetic deletion renders Mtb unable to persist in mice. Here, we report a series of macrocyclic peptides that potently and selectively target the Mtb20S over human proteasomes, including macrocycle . The cocrystal structure of macrocycle with Mtb20S revealed structural bases for the species selectivity. Inhibition of 20S within Mtb by dose dependently led to the accumulation of Pup-tagged GFP that is degradable but resistant to depupylation and death of nonreplicating Mtb under nitrosative stress. These results suggest that compounds of this class have the potential to develop as anti-TB therapeutics.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00296DOI Listing
May 2021

Accuracy and deviation analysis of robot-assisted spinal implants: A retrospective overview of 105 cases and preliminary comparison to open freehand surgery in lumbar spondylolisthesis.

Int J Med Robot 2021 May 4:e2273. Epub 2021 May 4.

Department of Spine Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.

Background: Whether the accuracy of robot-assisted spinal screw placement is significantly higher than that of freehand and the source of robotic deviation remain unclear.

Methods: Clinical data of 105 patients who underwent robot-assisted spinal surgery was collected, and screw accuracy was evaluated by computed tomography according to the modified Gertzbein-Robbins classification. Patients were grouped by percutaneous and open surgery. Intergroup comparisons of clinical and screw accuracy parameters were performed. Reasons for deviation were determined. Thirty-one patients with lumbar spondylolisthesis undergoing open robot-assisted surgery and the same number of patients treated by open freehand surgery were compared for screw accuracy.

Results: Screw accuracy was not significantly different between the percutaneous and open groups in both intra- and postoperative evaluations. Tool skiving was identified as the main cause of deviation. The proportion of malpositioned screws (grade B + C + D) was significantly higher in the freehand group than in the robot-assisted group. However, remarkably malpositioned (grade C + D) screws showed no significant differences between the groups. No revision surgery was necessary.

Conclusions: Robot-assisted spinal instrumentation manifests high accuracy and low incidence of nerve injury. Tool skiving is a major cause of implant deviation.
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http://dx.doi.org/10.1002/rcs.2273DOI Listing
May 2021

Stable osteosynthesis of cage in cage technique for surgical treatment of proximal humeral fractures.

BMC Surg 2021 May 4;21(1):233. Epub 2021 May 4.

Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, China.

Background: The treatment of a displaced proximal humeral fracture is still a matter of controversy. The purpose of this study was to report outcomes at a long-term follow-up after fixation augmentation using peek (polyether-ether-ketone) cage and locking compression plate (LCP).

Methods: A total of 27 patients (average age 53.8 years, range 19-86 years) were treated with peek cage and LCP. All of them had a minimum radiographic and clinical follow-up of 1 years. Outcomes were assessed using the Constant-Murley score (CMS), disability of the arm, shoulder and hand (DASH) score. Complications were also recorded during follow-up.

Results: The average follow-up was 28 months (range 12-48 months). The mean functional outcomes were as follows: CMS, 73.3 (range 61-86); DASH, 45.9 (range 27-68). A total of 4 patients had complications: osteonecrosis developed in one patient, loss of reduction was observed in 1 patient and stiffness was occurred in two patients.

Conclusion: The use of peek cage and LCP has been a valuable option in the treatment of proximal humeral fractures. The complication rate was acceptable. Suitable void filler in the proximal humerus for reconstructing the medial column integrity attains mechanical stability in reducing the incidence of the complications.
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http://dx.doi.org/10.1186/s12893-021-01235-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094560PMC
May 2021

Contamination and translocation of deoxynivalenol and its derivatives associated with Fusarium crown rot of wheat in Northern China.

Plant Dis 2021 May 4. Epub 2021 May 4.

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, Beijing, China;

Fusarium crown rot (FCR) is one of the most important wheat diseases in Northern China. The main causal agent of FCR, , can produce mycotoxins such as type B trichothecenes. Therefore, FCR could be an additional source of mycotoxin contamination during wheat production. Field inoculation experiments demonstrated that FCR disease severity strongly impacts the distribution pattern of trichothecenes in different wheat tissues. Mycotoxins were mainly observed in lower internodes and a low amount was detected in the upper parts above the 4th internode. However, high levels of trichothecene accumulation were detected in the upper segments of wheat plants under field conditions, which would threaten the feed production. The variation of mycotoxin content among sampling sites indicated that besides disease severity, other factors like climate, irrigation, and fungicide application may influence the mycotoxin accumulation in wheat. A comprehensive survey of DON and its derivatives in wheat heads with FCR symptoms in natural fields was conducted in 80 sites of seven provinces in Northern China. Much higher levels of mycotoxin were observed than those in inoculation experiments. The mycotoxin content varied greatly among sampling sites, but no significant differences were observed if compared at province level, which indicated that the variation is mainly due to local conditions. Trace amounts of mycotoxin appeared to be translocated to grains, indicating that FCR infection in natural fields poses a relatively small threat to contamination of grains, but a larger amount to plant parts that may be used as animal feed. To our knowledge, this is the first report of trichothecene accumulation in wheat stems, heads, and grains following FCR infection in natural field condition. These investigations provide novel insights into food and feed safety risk caused by FCR in Northern China.
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http://dx.doi.org/10.1094/PDIS-03-21-0612-REDOI Listing
May 2021

Current Status of Training and Certification for Congenital Heart Surgery Around the World: Proceedings of the Meetings of the Global Council on Education for Congenital Heart Surgery of the World Society for Pediatric and Congenital Heart Surgery.

World J Pediatr Congenit Heart Surg 2021 May;12(3):394-405

Division of Cardiovascular Surgery, Children's Minnesota, Minneapolis, MN, USA.

The optimal training of the highly specialized congenital heart surgeon is a long and complex process, which is a significant challenge in most parts of the world. The World Society for Pediatric and Congenital Heart Surgery (WSPCHS) has established the Global Council on Education for Congenital Heart Surgery as a nonprofit organization with the goal of assessing current training and certification and ultimately establishing standardized criteria for the training, evaluation, and certification of congenital heart surgeons around the world. The Global Council and the WSPCHS have reviewed the present status of training and certification for congenital cardiac surgery around the world. There is currently lack of consensus and standardized criteria for training in congenital heart surgery, with significant disparity between continents and countries. This represents significant obstacles to international job mobility of competent congenital heart surgeons and to the efforts to improve the quality of care for patients with Congenital Heart Disease worldwide. The purpose of this article is to summarize and document the present state of training and certification in congenital heart surgery around the world.
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http://dx.doi.org/10.1177/21501351211003520DOI Listing
May 2021

Dynamic monitor of CT scan within short interval in invasive pulmonary aspergillosis for nonneutropenic patients: a retrospective analysis in two centers.

BMC Pulm Med 2021 Apr 30;21(1):142. Epub 2021 Apr 30.

Key Laboratory of Respiratory Disease of Zhejiang Province, Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Background: In nonneutropenic patients with underlying respiratory diseases (URD), invasive pulmonary aspergillosis (IPA) is a life-threatening disease. Yet establishing early diagnosis in those patients remains quite a challenge.

Methods: A retrospective series of nonneutropenic patients with probable or proven IPA were reviewed from January 2014 to May 2018 in Department of Respiratory Medicine of two Chinese hospitals. Those patients were suspected of IPA and underwent lung computed tomography (CT) scans twice within 5-21 days. The items required for IPA diagnosis were assessed by their host factors, mycological findings and CT scans according to the European Organization for Research and Treatment of Cancer (EORTC) and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (MSG) criteria (EORTC/MSG criteria).

Results: Together with the risk factors, mycological findings and nonspecific radiological signs on first CT, ten patients were suspected of IPA. With the appearance of cavities on second CT scan in the following days, all patients met the criteria of probable or possible IPA. Except one patient who refused antifungal treatment, nine patients received timely antifungal treatment and recovered well. One of the nine treated IPA cases was further confirmed by pathology, one was confirmed by biopsy.

Conclusions: Dynamic monitor of CT scan provided specific image evidences for IPA diagnosis. This novel finding might provide a noninvasive and efficient strategy in IPA diagnosis with URD.
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http://dx.doi.org/10.1186/s12890-021-01512-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091757PMC
April 2021

Insights into the functional divergence of the haloacid dehalogenase superfamily from phosphomonoesterase to inorganic pyrophosphatase.

Arch Biochem Biophys 2021 Apr 30;705:108896. Epub 2021 Apr 30.

Biomedical Research Center, College of Life Science and Engineering, Northwest Minzu University, Lanzhou, 730030, PR China. Electronic address:

The evolution of enzyme catalytic structures and mechanisms has drawn increasing attention. In this study, we investigate the functional divergence from phosphomonoesterase to inorganic pyrophosphatase in the haloacid dehalogenase (HAD) superfamily. In this study, a series of models was constructed, and calculations were performed by using density functional theory with the B3LYP functional. The calculations suggest that in most HAD members, the active-site structure is unstable due to the binding of the substrate inorganic pyrophosphate (PPi), and reactions involving PPi cannot be catalyzed. In BT2127, which is a unique member of the HAD superfamily, the Mg-coordinating residues Asn172 and Glu47 play a role in stabilizing the active-site structure to adapt to the substrate PPi by providing much stronger coordination interactions with the Mg ion. The calculation results suggest that Asn172 and Glu47 are crucial in the evolution of the inorganic pyrophosphatase activity in the HAD superfamily. Our study provides definitive chemical insight into the functional divergence of the HAD superfamily, and helps in understanding the evolution of enzyme catalytic structures and mechanisms.
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http://dx.doi.org/10.1016/j.abb.2021.108896DOI Listing
April 2021

Initiation of efficient C pathway in response to low ambient CO during the bloom period of a marine dinoflagellate.

Environ Microbiol 2021 May 2. Epub 2021 May 2.

State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen, 361005, China.

Dinoflagellates are important primary producers and major causative agents of harmful algal blooms in the global ocean. Despite the great ecological significance, the photosynthetic carbon acquisition by dinoflagellates is still poorly understood. The pathways of photosynthetic carbon assimilation in a marine dinoflagellate Prorocentrum donghaiense under both in situ and laboratory-simulated bloom conditions were investigated using a combination of metaproteomics, qPCR, stable carbon isotope and targeted metabolomics approaches. A rapid consumption of dissolved CO to generate high biomass was observed as the bloom proceeded. The carbon assimilation genes and proteins including intracellular carbonic anhydrase 2, phosphoenolpyruvate carboxylase, phosphoenolpyruvate carboxykinase and RubisCO as well as their enzyme activities were all highly expressed at the low CO level, indicating that C photosynthetic pathway functioned in the blooming P. donghaiense cells. Furthermore, δ C values and content of C compound (malate) significantly increased with the decreasing CO concentration. The transition from C to C pathway minimizes the internal CO leakage and guarantees efficient carbon fixation at the low CO level. This study demonstrates the existence of C photosynthetic pathway in a marine dinoflagellate and reveals its important complementary role to assist carbon assimilation for cell proliferation during the bloom period.
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http://dx.doi.org/10.1111/1462-2920.15545DOI Listing
May 2021