Publications by authors named "Hao Zhang"

6,335 Publications

  • Page 1 of 1

The in-situ release of algal bloom populations and the role of prokaryotic communities in their establishment and growth.

Water Res 2022 May 12;219:118565. Epub 2022 May 12.

Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), China. Electronic address:

Harmful algal blooms (HABs) may quickly travel and inoculate new water bodies via currents and runoff in estuaries. The role of in-situ prokaryotic communities in the re-establishment and growth of inoculated algal blooms remains unknown. A novel on-board incubation experiment was employed to simulate the sudden surge of algal blooms to new estuarine waters and reveal possible outcomes. A dinoflagellate (Amphidinium carterae) and a diatom species (Thalassiosira weissflogii) which had bloomed in the Pearl River Estuary (PRE) area were cultured to bloom densities and reintroduced back into PRE natural seawaters. The diatom showed better adaptation ability to the new environment and increased significantly after the incubation. Simultaneously, particle-attached (PA) prokaryotic community structure was strongly influenced by adding of the diatom, with some opportunistic prokaryotes significantly enhanced in the diatom treatment. Whereas the dinoflagellate population did not increase following incubation, and their PA prokaryotic community showed no significant differences relative to the control. Metagenomic analyzes revealed that labile carbohydrates and organic nitrogen produced by the diatom contributed to the surge of certain PA prokaryotes. Genomic properties of a bacteria strain, which is affiliated with genus GMD16E07 (Planctomycetaceae) and comprised up to 50% of PA prokaryotes in the diatom treatment, was described here for the first time. Notably, the association of Planctomycetaceae and T. weissflogii likely represents symbiotic mutualism, with the diatom providing organic matter for Planctomycetaceae and the bacteria supplying vitamins and detoxifying nitriles and hydrogen peroxides in exchange. Therefore, the close association between Planctomycetaceae and T. weissflogii promoted the growth of both populations, and eventually facilitated the diatom bloom establishment.
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http://dx.doi.org/10.1016/j.watres.2022.118565DOI Listing
May 2022

Radix Ranunculi ternati: review of its chemical constituents, pharmacology, quality control and clinical applications.

J Pharm Pharmacol 2022 May 21. Epub 2022 May 21.

School of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.

Objectives: Radix ranunculi ternati (RRT) is widely used as traditional and folk medicine distributed in China, as well as in Kyushu and Shikoku in Japan. Although RRT has a short history of use as medicine, it has a high medicinal value. This study summarizes the research progress on the chemical constituents, pharmacological activity, quality control measures and clinical applications to provide a valuable and exhaustive reference for the development and application of RRT.

Key Findings: Phytochemical studies showed that this plant mainly contains glycosides, organic acids, esters, sterols, flavonoids, alkaloids and volatile oils. The pharmacological activity of RRT includes immune regulation, anti-tumour, anti-tuberculosis, antioxidant, antibacterial, hepatoprotective and anti-inflammatory effects. This plant is especially effective in the treatment of cancer, tuberculosis, thyroid and nasopharynges disorders.
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http://dx.doi.org/10.1093/jpp/rgac018DOI Listing
May 2022

Enhanced accumulation of oil through co-expression of fatty acid and ABC transporters in Chlamydomonas under standard growth conditions.

Biotechnol Biofuels Bioprod 2022 May 20;15(1):54. Epub 2022 May 20.

School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.

Background: Chloroplast and endoplasmic reticulum (ER)-localized fatty acid (FA) transporters have been reported to play important roles in oil (mainly triacylglycerols, TAG) biosynthesis. However, whether these FA transporters synergistically contribute to lipid accumulation, and their effect on lipid metabolism in microalgae are unknown.

Results: Here, we co-overexpressed two chloroplast-localized FA exporters (FAX1 and FAX2) and one ER-localized FA transporter (ABCA2) in Chlamydomonas. Under standard growth conditions, FAX1/FAX2/ABCA2 over-expression lines (OE) accumulated up to twofold more TAG than the parental strain UVM4, and the total amounts of major polyunsaturated FAs (PUFA) in TAG increased by 4.7-fold. In parallel, the total FA contents and major membrane lipids in FAX1/FAX2/ABCA2-OE also significantly increased compared with those in the control lines. Additionally, the total accumulation contribution ratio of PUFA, to total FA and TAG synthesis in FAX1/FAX2/ABCA2-OE, was 54% and 40% higher than that in UVM4, respectively. Consistently, the expression levels of genes directly involved in TAG synthesis, such as type-II diacylglycerol acyltransferases (DGTT1, DGTT3 and DGTT5), and phospholipid:diacylglycerol acyltransferase 1 (PDAT1), significantly increased, and the expression of PGD1 (MGDG-specific lipase) was upregulated in FAX1/FAX2/ABCA2-OE compared to UVM4.

Conclusion: These results indicate that the increased expression of FAX1/FAX2/ABCA2 has an additive effect on enhancing TAG, total FA and membrane lipid accumulation and accelerates the PUFA remobilization from membrane lipids to TAG by fine-tuning the key genes involved in lipid metabolism under standard growth conditions. Overall, FAX1/FAX2/ABCA2-OE shows better traits for lipid accumulation than the parental line and previously reported individual FA transporter-OE. Our study provides a potential useful strategy to increase the production of FA-derived energy-rich and value-added compounds in microalgae.
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http://dx.doi.org/10.1186/s13068-022-02154-6DOI Listing
May 2022

The "Hand as Foot" teaching method in the lumbar anatomy and pedicle screw-rod system.

Asian J Surg 2022 May 18. Epub 2022 May 18.

Inner Mongolia Medical University, Department of Orthopedics, Affiliated Hospital of Inner Mongolia Medical University, Huhehot North Street, Inner Mongolia, 010050, China.

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http://dx.doi.org/10.1016/j.asjsur.2022.04.135DOI Listing
May 2022

Clinical Risk Factors for Thoracic Ossification of the Ligamentum Flavum: A Cross-Sectional Study Based on Spinal Thoracic Three-Dimensional Computerized Tomography.

Risk Manag Healthc Policy 2022 13;15:1065-1072. Epub 2022 May 13.

Department of Spine Surgery, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.

Background: Inconsistent results of the clinical risk factors associated with thoracic ossification of the ligamentum flavum (TOLF) have been reported in limited previous studies.

Purpose: This retrospective study aimed to investigate the potential risk factors for TOLF by a retrospective cross-sectional study, which may provide valuable experience for further clinical and pathophysiological research.

Methods: A total of 2247 asymptomatic participants, who underwent spinal thoracic three-dimensional computerized tomography (3D-CT) scans at our institution from January 2016 to December 2019, were enrolled in this study according to the screening criteria. Demographic information such as age, sex, height, weight, body mass index (BMI), smoking and drinking history, diastolic blood pressure (DBP), systolic blood pressure (SBP), and pulse pressure (PP) were recorded. Laboratory results included serum low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC), triglyceride (TG), uric acid (UA), creatinine (Cr), calcium, and phosphorus. Participants were divided into TOLF group and non-TOLF group in accordance with the thoracic 3D-CT manifestation.

Results: TOLF was observed in 153 (6.81%) asymptomatic participants. Comparison of demographic data and laboratory examinations between the two groups showed that participants in the TOLF group were older, had a higher BMI, as well as higher levels of DBP. In addition, there was no significant difference in sex, drinking, tobacco use, SBP, TC, TG, PP, and levels of LDL-C, HDL-C, sUA, sCr, calcium, and phosphorus between the two groups. Furthermore, dichotomous logistic regression analyses revealed that age (OR = 1.018, p = 0.041) and BMI (OR = 1.090, p < 0.001) were risk factors for TOLF.

Conclusion: Our study reveals that age and BMI are clinical risk factors for the development of TOLF, while age cannot be identified as an independent risk factor for female in subgroup analysis.
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http://dx.doi.org/10.2147/RMHP.S361730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113497PMC
May 2022

Exchange between Interlayer and Intralayer Exciton in WSe/WS Heterostructure by Interlayer Coupling Engineering.

Nano Lett 2022 May 19. Epub 2022 May 19.

Key Laboratory of Flexible Electronics & Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, China.

Because of type-II band alignment, interlayer exciton (IX) is found in a van der Waals (vdW) heterostructure (HS) formed by two monolayers of transition-metal dichalcogenides. Manipulation of IXs is of great importance for excitonic integrated devices. Here, we demonstrate that high pressure and tensile strain can be applied to enhance and reduce interlayer coupling of WSe/WS HS, respectively. High pressure induces the transform of intralayer excitons to IX, while tensile strain leads to the transform of IXs to intralayer excitons. In addition, there is a direct-to-indirect band gap transition of WSe/WS HS. The interlayer distance of WSe/WS HS is reduced under high pressure, but it increased under uniaxial tensile strain from first-principles calculations. The calculated band structures explain well the transformation between interlayer and intralayer excitons of WSe/WS HS. This work demonstrates the exchange of interlayer and intralayer excitons and paves the way to manipulate excitons of HS for excitonic applications.
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http://dx.doi.org/10.1021/acs.nanolett.2c01353DOI Listing
May 2022

[Human resources of community oral public health service and job satisfaction of the staff in Shanghai].

Shanghai Kou Qiang Yi Xue 2022 Feb;31(1):79-83

Department of Preventive Dentistry, Shanghai Stomatological Hospital, Fudan University. Shanghai 200001, China. E-mail:

Purpose: To investigate the human resources of oral public health system and staff's job satisfaction in Shanghai, and to provide basis for improving the services of oral public health.

Methods: A census was performed among 246 health service centers in Shanghai from February to March 2019, and a questionnaire on oral public health staff's basic information of and job satisfaction was conducted. SPSS 20.0 software package was used for statistical analysis of job satisfaction and basic situation of employees.

Results: There were 759 community oral health service personnel in Shanghai, mainly composed of dentists (34.0%), public health physicians (21.96%), nurses (31.6%), and 24 general practitioners(3.2%) were also found. The profile of the staff was mainly 30-49 years old (69.8%), with bachelor degree (66.7%) and primary and intermediate professional titles (91.7%). Only 14.8% of them spend more than 24 hours in oral public health work every week. Community oral health workers had higher satisfaction with their superiors and colleagues, and lower satisfaction with their work income. Age, levels of professional title and years of oral health service were negatively correlated with job satisfaction.

Conclusions: It is necessary to build a proper human resource system of community oral public health and improve the treatment of the staff, in order to promote the oral public health services in Shanghai.
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February 2022

activates the host RIDD pathway to subvert BLOS1-directed immune defense.

Elife 2022 May 19;11. Epub 2022 May 19.

Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center, Bryan, United States.

The phagocytosis and destruction of pathogens in lysosomes constitute central elements of innate immune defense. Here, we show that , the causative agent of brucellosis, the most prevalent bacterial zoonosis globally, subverts this immune defense pathway by activating regulated IRE1α-dependent decay (RIDD) of mRNA encoding BLOS1, a protein that promotes endosome-lysosome fusion. RIDD-deficient cells and mice harboring a RIDD-incompetent variant of IRE1α were resistant to infection. Inactivation of the gene impaired the ability to assemble BLOC-1-related complex (BORC), resulting in differential recruitment of BORC-related lysosome trafficking components, perinuclear trafficking of -containing vacuoles (BCVs), and enhanced susceptibility to infection. The RIDD-resistant variant maintains the integrity of BORC and a higher-level association of BORC-related components that promote centrifugal lysosome trafficking, resulting in enhanced BCV peripheral trafficking and lysosomal destruction, and resistance to infection. These findings demonstrate that host RIDD activity on BLOS1 regulates intracellular parasitism by disrupting BORC-directed lysosomal trafficking. Notably, coronavirus murine hepatitis virus also subverted the RIDD-BLOS1 axis to promote intracellular replication. Our work establishes BLOS1 as a novel immune defense factor whose activity is hijacked by diverse pathogens.
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http://dx.doi.org/10.7554/eLife.73625DOI Listing
May 2022

Nomogram to Predict the Risk of Postoperative Anxiety and Depression in Colorectal Cancer Patients.

Int J Gen Med 2022 12;15:4881-4895. Epub 2022 May 12.

Department of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, People's Republic of China.

Purpose: To develop and validate the risk nomogram to predict the likelihood of postoperative anxiety and depression in colorectal cancer (CRC) patients.

Methods: A total of 602 CRC patients from the Second Affiliated Hospital of Harbin Medical University were included in the study and divided into development set and validation set with the 2:1 ratio randomly. Logistic regression model was used to determine independent factors contributing to postoperative anxiety and depression, which were subsequently applied to build the nomogram for predicting postoperative anxiety and depression. The performance of the risk nomogram was appraised by the area under the receiver operating curve (AUC), calibration curves and decision curve analyses (DCA).

Results: Gender, personal status, income, adjuvant therapy, the Eastern Cooperative Oncology Group Scale (ECOG) score, comorbidity, postoperative complications and stoma status were significant indicators for postoperative anxiety and depression. The AUCs for the development and validation sets were 0.792 and 0.812 for the postoperative anxiety nomogram and 0.805 and 0.825 for the postoperative depression nomogram. Additionally, calibration curves and decision curve analyses also determined the reliable clinical importance of the proposed nomogram.

Conclusion: The current study constructed the risk nomogram for postoperative anxiety and depression and could help clinicians determine high-risk patients to some extent.
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http://dx.doi.org/10.2147/IJGM.S350092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109807PMC
May 2022

Transcriptomic characterization of Atlantic salmon (Salmo salar) head kidney following administration of Aeromonas salmonicida subsp. masoucida vaccine.

Fish Shellfish Immunol 2022 May 14. Epub 2022 May 14.

School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address:

Atlantic salmon is one of the most famous and economically important fish species globally. However, bacterial diseases constantly constrain salmon aquaculture. Thereinto, Aeromonas salmonicida subsp. masoucida (ASM), classified as atypical A. salmonicida, caused huge losses to salmonid industry in China. In this regard, we conducted transcriptome analysis in Atlantic salmon head kidney following the administration of ASM vaccination to reveal genes, their expression patterns, and pathways involved in immune responses. A total of 448.71 million clean reads were obtained, and 397.69 million reads were mapped onto the Atlantic salmon reference genome. In addition, 117, 1891, 741, 207, and 377 genes were significantly up-regulated, and 183, 1920, 695, 83, and 539 genes were significantly down-regulated post ASM vaccination at 12 h, 24 h, 1 m, 2 m, and 3 m, respectively. Furthermore, KEGG pathway analysis revealed that many differentially expressed genes (DEGs) following ASM vaccination were involved in cell adhesion molecules (H2-Aa-l and CD28-l),cytokine-cytokine receptor interaction (IL10, CXCL9, CXCL11, CXCR3, and CCL19), herpes simplex infection (IL1B, SOCS3-l, and C3-l), HTLV-I infection (Il1r2 and BCL2L1), influenza A (CXCL8 and Il12b), and PI3K-Akt signaling pathway (PIK3R3-l and Ddit4-l). Finally, the results of qRT-PCR showed a significant correlation with RNA-Seq results, suggesting the reliability of RNA-Seq for gene expression analysis. This study sets the foundation for further study on the vaccine protective mechanism in Atlantic salmon as well as other teleost species.
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http://dx.doi.org/10.1016/j.fsi.2022.05.022DOI Listing
May 2022

Effects of magnolol and honokiol blend on performance, egg quality, hepatic lipid metabolism, and intestinal morphology of hens at late laying cycle.

Animal 2022 May 13;16(6):100532. Epub 2022 May 13.

Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Nanhu Road, No. 1 of Nanhuyaoyuan, 430064 Wuhan, China. Electronic address:

Magnolol and its isomer honokiol are polyphenols with anti-oxidative and anti-inflammatory activities. We evaluated the effects of magnolol and honokiol supplementation alone or in combination with hen diets during the late laying cycle. A total of 540 Jingfen pink-shell laying hens (50 weeks old) were randomly assigned to six treatments: a control diet and diets supplemented with 300 mg/kg magnolol (M), honokiol (H), or 300 mg/kg total phenols with a magnolol/honokiol ratio of 2:1 (MH), 1:2 (MH), and 1:1 (MH). Compared with that of the control, all supplementation groups had higher laying rates and the M, MH, and MH groups showed comparatively lower feed conversion ratios. Magnolol and honokiol supplementation increased the Haugh units of fresh eggs at week 62 and alleviated the decline of the Haugh units of eggs stored for 14 days. Compared with that of the control group, the serum total antioxidant capacity of the MH and MH groups significantly increased, and all supplementation groups had higher total antioxidant capacity and lower malondialdehyde content in the liver. With respect to lipid metabolism, the MH and MH groups had lower total and relative liver weights compared with those of the control and H groups. The mRNA expression levels of CCAAT enhancer binding protein alpha, sterol regulatory element binding protein-1, fatty acid synthase and stearyl coenzyme A desaturase 1 involved in lipogenesis; microsomal triglyceride transfer protein and apolipoprotein B involved in fatty acid transport; and the proinflammatory cytokine interleukin-1 beta were lower in all supplementation groups compared with those in the control. With respect to gut health, the heights of the jejunum and ileum villi significantly increased in all supplementation groups compared with those of the control, and the jejunum villus heights of the M and MH groups were higher than those of the H and MH groups. The H and MH groups had higher mRNA expression levels of zonula occludens-1 in the ileum compared with those in the control and M groups, whereas all supplementation groups had higher mRNA levels of claudin-1 than that of the control group. In conclusion, magnolol and honokiol improved hen performance and the albumen quality of fresh and stored eggs by improving the antioxidant capacity, liver lipid metabolism, and intestinal health of laying hens. The combination of magnolol and honokiol at a 1:1 ratio may be an optimal choice for hen diet supplementation.
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http://dx.doi.org/10.1016/j.animal.2022.100532DOI Listing
May 2022

Advances in the Study of Circadian Genes in Non-Small Cell Lung Cancer.

Integr Cancer Ther 2022 Jan-Dec;21:15347354221096080

Tianjin Medical University General Hospital, Tianjin, China.

Circadian genes regulate several physiological functions such as circadian rhythm and metabolism and participate in the cytogenesis and progression of various malignancies. The abnormal expression of these genes in non-small cell lung cancer (NSCLC) is closely related to the clinicopathological features of NSCLC and may promote or inhibit NSCLC progression. Circadian rhythm disorders and clock gene abnormalities may increase the risk of lung cancer in some populations. We collected 15 circadian genes in NSCLC, namely , and , and determined their relationships with the clinicopathological features of patients and the potential mechanisms promoting or inhibiting NSCLC progression. We also summarized the studies on circadian rhythm disorders and circadian genes associated with lung cancer risk. The present study aimed to provide theoretical support for the future exploration of new therapeutic targets and for the primary prevention of NSCLC from the perspective of circadian genes. Interpretation of circadian rhythms in lung cancer could guide further lung cancer mechanism research and drug development that could lead to more effective treatments and improve patient outcomes.
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http://dx.doi.org/10.1177/15347354221096080DOI Listing
May 2022

Residual Risk of Coronary Atherosclerotic Heart Disease and Severity of Coronary Atherosclerosis Assessed by ApoB and LDL-C in Participants With Statin Treatment: A Retrospective Cohort Study.

Front Endocrinol (Lausanne) 2022 20;13:865863. Epub 2022 Apr 20.

Department of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Low-density lipoprotein cholesterol (LDL-C) is the primary target of lipid-lowering therapy on the management of hypercholesterolemia in the United States and European guidelines, while apolipoprotein B (apoB) is the secondary target. The objective was to determine if elevated levels of apoB is superior to LDL-C in assessing residual risk of coronary atherosclerotic heart disease and severity of coronary atherosclerosis in participants with statin treatment.

Methods: This study included 131 participants with statin treatment. The generalized linear model and relative risk regression (generalized linear Poisson model with robust error variance) were used to analyze the association of the levels of apoB and LDL-C with the severity of coronary atherosclerosis and residual risk of coronary atherosclerotic heart disease.

Results: Categorizing apoB and LDL-C based on tertiles, higher levels of apoB were significantly associated with the severity of coronary atherosclerosis ( = 0.012), whereas no such associations were found for elevated levels of LDL-C ( = 0.585). After multivariate adjustment, higher levels of apoB were significantly associated with residual risk of coronary atherosclerotic heart disease. When compared with low-level apoB (≤0.66 g/L), the multivariate adjusted RR and 95% CI of intermediate-level apoB (0.67-0.89 g/L) and high-level apoB (≥0.90 g/L) were 1.16 (1.01, 1.33) and 1.31 (1.08, 1.60), respectively ( = 0.011). There was a 45% increased residual risk of coronary atherosclerotic heart disease per unit increment in natural log-transformed apoB ( <0.05). However, higher levels of LDL-C were not significantly associated with residual risk of coronary atherosclerotic heart disease. When compared with low-level LDL-C (≤1.56 mmol/L), the multivariate adjusted RR and 95% CI of intermediate-level LDL-C (1.57-2.30 mmol/L) and high-level LDL-C (≥2.31 mmol/L) were 0.99 (0.84, 1.15) and 1.10 (0.86, 1.42), respectively ( = 0.437). Similar results were observed in the stratified analyses and sensitivity analyses. No significant interactions were detected for both apoB and LDL-C (all 0.05).

Conclusions: Elevated apoB are superior in assessing the residual risk of coronary atherosclerotic heart disease and severity of coronary atherosclerosis in participants with statin treatment.
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http://dx.doi.org/10.3389/fendo.2022.865863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097510PMC
May 2022

Treated by Electrostatic Spray Drying Relieved Constipation by Changing the Relative Abundance of Bacteria Associated With Gastrointestinal Regulatory Peptides.

Front Cell Infect Microbiol 2022 27;12:894216. Epub 2022 Apr 27.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.

In this study, three different microencapsulation methods were used to embed to explore the alleviating effects of embedding methods on constipated mice. By measuring the defecation-related parameters, it was found that the Bifidobacteria treated by electrostatic spray drying had the best ability to relieved constipation. Furthermore, by detecting constipation-related gastrointestinal regulatory peptides, inflammatory factors, intestinal microbiota, and SCFAs, it was discovered that Bifidobacteria treated by electrostatic spray drying changed the composition of intestinal microbiota, especially the relative abundance of bacteria that were positively correlated with AQP3, but negatively correlated with ET-1 and SS, then increased the level of AQP3 in the intestine, and finally relieved constipation by increasing the fecal water content and small intestinal propulsion rate. In conclusion, the electrostatic spray drying method was superior to the other two methods in maintaining the activity of Bifidobacteria and relieved constipation by changing the relative abundance of bacteria that were correlated with gastrointestinal regulatory peptides and increasing the content of fecal water and small intestinal propulsion rate.
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http://dx.doi.org/10.3389/fcimb.2022.894216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094687PMC
May 2022

Effect of Gastrointestinal Hemorrhage on Outcome After Endovascular Treatment in Acute Basilar Artery Occlusion.

Front Neurol 2022 28;13:809209. Epub 2022 Apr 28.

Department of Neurology, Wuhan No. 1 Hospital, Wuhan, China.

Background And Purpose: Gastrointestinal hemorrhage (GIH) is associated with a poorer prognosis and a higher mortality rate after acute ischemic stroke (AIS), but its association with outcomes after endovascular treatment (EVT) remains unclear. This study aimed to assess the incidence, risk factors, and relationships among clinical outcomes of GIH after EVT in patients with acute basilar artery occlusion (BAO).

Methods: Consecutive patients treated with EVT were identified from the EVT for Acute Basilar Artery Occlusion Study (BASILAR) registry. All enrolled patients were divided into GIH and non-GIH subgroups, and the independent predictors of GIH after EVT were explored. An ordinal logistic regression model was used to assess the association between GIH and primary outcome [distribution of modified Rankin scale (mRS)] at 90 days, while binary logistic regression models for other outcomes were also employed.

Results: Among 647 patients with acute BAO, 114 (17.6%) patients experienced GIH after EVT. Higher glucose levels at admission, longer procedure time, and general anesthesia were the independent predictors of GIH after EVT, while protective factors include the posterior circulation-Acute Stroke Prognosis Early Computed Tomography Score (pc-ASPECTS) ≥ 5 and a history of hyperlipidemia. Compared with the non-GIH group, the GIH group was associated with a worse functional outcome [adjusted common odds ratio (OR), 2.12 (95% CI, 1.39-3.25)], lower rates of functional independence [adjusted OR,.47 (95% CI, 0.26-0.88)], a favorable outcome [adjusted OR, 0.41 (95% CI, 0.22-0.73)], and a higher risk of 90-day mortality [adjusted OR, 1.76 (95% CI, 1.08-2.85)].

Conclusion: This study concluded that GIH is not uncommon after EVT in patients with acute BAO and is associated with worse functional disability and higher mortality.
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http://dx.doi.org/10.3389/fneur.2022.809209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100889PMC
April 2022

Cardiac-type total anomalous pulmonary venous return is not benign.

J Thorac Cardiovasc Surg 2022 Apr 19. Epub 2022 Apr 19.

Department of Cardio-thoracic Surgery, Congenital Heart Center, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Objective: The objective of this study was to investigate the association between morphological variation and postsurgical pulmonary vein (PV) stenosis (PPVS) in patients with cardiac total anomalous pulmonary venous connection (TAPVC).

Methods: This single-center, retrospective study included 168 pediatric patients who underwent surgical repair of cardiac TAPVC from 2013 to 2019 (connection to the coronary sinus [CS], n = 136; connection directly to the right atrium [RA], n = 32). Three-dimensional computed tomography modeling and geometric analysis were performed to investigate the morphological features; their relevance to the PPVS was examined.

Results: The connection type had no association with PPVS (CS type: 18% vs right atrial type: 19%; P = .89) but there was a higher incidence of PPVS in patients with a single PV orifice than > 1 orifice (P < .001). Confluence-to-total PV area ratio (hazard ratio, 4.78, 95% CI, 1.86-12.32; P = .001) and length of drainage route (hazard ratio, 1.22; 95% CI, 1.14-1.31; P < .001) had a 4- and 1-fold increase in the risk for PPVS in the CS type after adjustment for age and preoperative pulmonary venous obstruction. In the right atrial type, those with anomalous PV return to the RA roof were more likely to develop PPVS than to the posterior wall of the RA (P < .001).

Conclusions: The number of inter-junction PV orifice correlated with PPVS development in cardiac TAPVC. The confluence-to-total PV ratio, length of drainage route, and anomalous PV return to the RA roof are important predictors for PPVS. Morphological subcategorization in this clinical setting can potentially assist in surgical decision-making.
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http://dx.doi.org/10.1016/j.jtcvs.2022.03.036DOI Listing
April 2022

A Structure-Activity Relationship Study of the Inhibition of α-Amylase by Benzoic Acid and Its Derivatives.

Nutrients 2022 May 5;14(9). Epub 2022 May 5.

Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Phenolic acids are widely found in fruits and vegetables. The inhibitory effect of phenolic acids on α-amylase, a key enzyme for starch digestion, has attracted the attention of researchers. To further investigate the effects of different substituents on the benzene ring of phenolic acid on the inhibition of α-amylase activity, in vitro experiments and molecular docking were used. The structure-activity relationships of 17 phenolic acids with benzoic acid as the parent nucleus were analyzed by determining their half inhibitory concentration (IC) toward α-amylase. The results showed that 2,3,4-trihydroxybenzoic acid had the strongest inhibitory effect on α-amylase with an IC value of 17.30 ± 0.73 mM. According to the structure-activity analysis, the hydroxyl group at the 2-position on the benzene ring had a strong positive effect on the inhibitory activity of α-amylase, while methoxylation at the 2-position and hydroxylation at the 5-position had a negative effect. Molecular docking revealed that hydrogen bonding and hydrophobic interactions were involved in the inhibition, with hydrogen bonding being the primary force. These findings provide a more comprehensive understanding of phenolic acids as inhibitors of α-amylase and provide new ideas for the design of dietary formulations for diabetic patients.
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http://dx.doi.org/10.3390/nu14091931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102017PMC
May 2022

Extract Regulates Gut Microbes and Metabolites to Attenuate Hepatic Inflammation and Lipid Metabolism Disorders via the AhR-FAS and NF-κB Signaling Pathways in Mice with Non-Alcoholic Fatty Liver Disease.

Nutrients 2022 Apr 19;14(9). Epub 2022 Apr 19.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Recent studies have shown that non-alcoholic fatty liver disease (NAFLD) is closely related to the gut microbiome. is widely used as a traditional seasoning and has a therapeutic effect on gastrointestinal diseases. Although previous studies have shown that extracts have anti-obesity and anti-diabetes effects in mice fed a high-fat diet, few studies have determined the active components or the corresponding mechanism in vivo. In this study, for the first time, an extract (MFE) was shown to be a prebiotic that regulates gut microbes and metabolites in mice fed a high-fat diet. Bioinformatics, network pharmacology, microbiome, and metabolomics analyses were used to analyze the nutrient-target pathway interactions in mice with NAFLD. The National Center for Biotechnology Information Gene Expression Omnibus database was used to analyze NAFLD-related clinical data sets to predict potential targets. The drug database and disease database were then integrated to perform microbiome and metabolomics analyses to predict the target pathways. The concentrations of inflammatory factors in the serum and liver, such as interleukin-6 and tumor necrosis factor-α, were downregulated by MFE. We also found that the hepatic concentrations of low-density lipoprotein cholesterol, total cholesterol, and triglycerides were decreased after MFE treatment. Inhibition of the nuclear factor kappa B (NF-κB) pathway and downregulation of the fatty acid synthase (FAS)-sterol regulatory element-binding protein 1c pathway resulted in the regulation of inflammation and lipid metabolism by activating tryptophan metabolite-mediated aryl hydrocarbon receptors (AhR). In summary, MFE effectively attenuated inflammation and lipid metabolism disorders in mice with NAFLD through the NF-κB and AhR-FAS pathways.
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http://dx.doi.org/10.3390/nu14091699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104743PMC
April 2022

Comparative Transcriptome Analysis Reveals Regulatory Mechanism of Long Non-Coding RNAs during Abdominal Preadipocyte Adipogenic Differentiation in Chickens.

Animals (Basel) 2022 Apr 24;12(9). Epub 2022 Apr 24.

National Engineering Laboratory for Animal Breeding, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

Long non-coding RNAs (lncRNAs) are implicated in mammalian adipogenesis and obesity. However, their genome-wide distribution, expression profiles, and regulatory mechanisms during chicken adipogenesis remain rarely understood. In the present study, lncRNAs associated with adipogenesis were identified from chicken abdominal adipocytes at multiple differentiation stages using Ribo-Zero RNA-seq. A total of 15,179 lncRNAs were identified and characterized by stage-specific expression patterns. Of these, 840 differentially expressed lncRNAs were detected, and their - and -target genes were significantly enriched in multiple lipid-related pathways. Through weighted gene co-expression network analysis (WGCNA) and time-series expression profile clustering analysis, 14 key lncRNAs were identified as candidate regulatory lncRNAs in chicken adipogenic differentiation. The - and -regulatory interactions of key lncRNAs were constructed based on their differentially expressed - and -target genes, respectively. We also constructed a competing endogenous RNA (ceRNA) network based on the key lncRNAs, differentially expressed miRNAs, and differentially expressed mRNAs. MSTRG.25116.1 was identified as a potential regulator of chicken abdominal preadipocyte adipogenic differentiation by acting as a transcriptional -regulator of fatty acid amide hydrolase () gene expression and/or a ceRNA that post-transcriptionally mediates gene expression by sponging gga-miR-1635.
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http://dx.doi.org/10.3390/ani12091099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101879PMC
April 2022

Stimulates Intestinal Secretion of Immunoglobulin A in an Individual-Specific Manner.

Foods 2022 Apr 25;11(9). Epub 2022 Apr 25.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Immunoglobulin A (IgA), as the most secreted immunoglobulin in the intestine, plays an irreplaceable role in mucosal immunity regulation. Previous studies have indicated that showed strain specificity in stimulating the secretion of IgA through intestinal mucosal lymphocytes. The reason for this phenomenon is not clear. The current studies have been aimed at exploring the effect of a strain on the secretion of IgA in the host's intestine, but the mechanism behind it has not been seriously studied. Based on this, we selected five strains of isolated from different individuals to determine whether there are intraspecific differences in stimulating the secretion of IgA from the intestinal mucosa. It was found that IgA concentrations in different intestinal segments and faeces induced by were different. 12-1 and X6L1 strains increased the secretion of IgA by the intestine significantly. In addition, different strains of were also proven to have different effects on the host gut microbiota but no significant effects on IgA-coated microbiota. Besides, it was speculated that different strains of may act on different pathways to stimulate IgA in a non-inflammatory manner. By explaining the differences of IgA secretion in the host's intestine tract stimulated by different strains of , it is expected to provide a theoretical basis for the stimulation of intestinal secretion of IgA by and a new direction for exploring the relationship between and human immunity.
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http://dx.doi.org/10.3390/foods11091229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099657PMC
April 2022

Metagenomic Analysis of the Effects of and Fructooligosaccharides (FOS) on the Fecal Microbiota Structure in Mice.

Foods 2022 Apr 19;11(9). Epub 2022 Apr 19.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Understanding the association between food composition and intestinal microbiota in the context of individual health is a critical problem in personalized nutrition. The objective of the present research was to elucidate the influence of ST-III and fructooligosaccharides (FOS) on the intestinal microbiota structure. We found that ST-III and FOS interventions remarkably enhanced the levels of cecal short-chain fatty acids (SCFAs), especially acetic, butyric, and valeric acids. Moreover, ST-III and/or FOS intervention obviously altered the intestinal microbiota structure. At the genus level, ST-III and/or FOS intervention remarkably elevated the proportion of , , , , and . Correlation analysis further uncovered that the specific compositional features of intestinal microbiota were strongly related to the concentration of cecal SCFAs. Our results offered scientific evidence to understanding the association between food composition and intestinal microbiota.
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http://dx.doi.org/10.3390/foods11091187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102988PMC
April 2022

The key sulfometuron-methyl degrading bacteria isolation based on soil bacterial phylogenetic molecular ecological networks and application for bioremediation of contaminated soil by immobilization.

Ecotoxicol Environ Saf 2022 Jun 10;238:113605. Epub 2022 May 10.

College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, PR China. Electronic address:

The analysis of soil bacterial community has guiding significance for fully utilization of soil microbial resources. The results of high-throughput sequencing (HTS) showed that the bacteria in the three sulfometuron-methyl contaminated soil samples were mainly composed of 677 genera, including Phenylobacterium, Bacillus, belonging to 28 phyla, including Proteobacteria, Firmicutes. The diversity and richness of bacterial community decreased with the increase in sulfometuron-methyl concentration. In addition, sulfometuron-methyl could also affect the soil bacterial function based on PICRUSt functional predictive analysis. Combined with the results of HTS and phylogenetic molecular ecological networks (pMENs), 12 genera, including Ralstonia (Pi=0.64), were identified as the key soil microflora (intra-module connectivity Zi ≥ 2.5 or inter-module connectivity Pi ≥ 0.62), and the abundance of Ralstonia significantly increased with the concentration of sulfometuron-methyl, indicating that the strains of this genus might be the potential degrading bacteria and could form a stable relationship with indigenous microorganisms. Among the isolated bacteria of genus Ralstonia, one strain, named Ralstonia sp. JM-1, was verified to possess higher sulfometuron-methyl degradation efficiency, which completely degraded 20 mg L of sulfometuron-methyl within 96 h. Furthermore, the immobilized strains generated by the mixture of 2.0 g bamboo charcoal and 3.0 mL bacterial suspension for 24 h had the highest sulfometuron-methyl degradation rate than that under other conditions, and the dynamic process degrading 10-30 mg L of sulfometuron-methyl conforms to the zero-order kinetic equation. The bioremediation of contaminated soil showed the immobilized strains could completely degrade sulfometuron-methyl (1.39 mg kg) in contaminated soil within 9 d, which is higher than that application of strains in the free state (74.8%). This study could provide ideas for the isolation of functional strains and a theoretical basis for the bioremediation of STM and other contaminated soils.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113605DOI Listing
June 2022

RabbitV: fast detection of viruses and microorganisms in sequencing data on multi-core architectures.

Bioinformatics 2022 May;38(10):2932-2933

School of Software, Shandong University, Jinan, China.

Motivation: Detection and identification of viruses and microorganisms in sequencing data plays an important role in pathogen diagnosis and research. However, existing tools for this problem often suffer from high runtimes and memory consumption.

Results: We present RabbitV, a tool for rapid detection of viruses and microorganisms in Illumina sequencing datasets based on fast identification of unique k-mers. It can exploit the power of modern multi-core CPUs by using multi-threading, vectorization and fast data parsing. Experiments show that RabbitV outperforms fastv by a factor of at least 42.5 and 14.4 in unique k-mer generation (RabbitUniq) and pathogen identification (RabbitV), respectively. Furthermore, RabbitV is able to detect COVID-19 from 40 samples of sequencing data (255 GB in FASTQ format) in only 320 s.

Availability And Implementation: RabbitUniq and RabbitV are available at https://github.com/RabbitBio/RabbitUniq and https://github.com/RabbitBio/RabbitV.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btac187DOI Listing
May 2022

Research progress and applications of silica-based aerogels - a bibliometric analysis.

RSC Adv 2022 May 11;12(22):14137-14153. Epub 2022 May 11.

Aerospace Institute of Advanced Material & Processing Technology Beijing 100074 P. R. China

Silica aerogels are three-dimensional porous materials that were initially produced in 1931. During the past nearly 90 years, silica aerogels have been applied extensively in many fields. In order to grasp the progress of silica-based aerogels, we utilize bibliometrics and visualization methods to analyze the research hotspots and the application of this important field. Firstly, we collect all the publications on silica-based aerogels and then analyze their research trends and performances by a bibliometric method regarding publication year/citation, country/institute, journals, and keywords. Following this, the major research hotspots of this area with a focus on synthesis, mechanical property regulation, and the applications for thermal insulation, adsorption, and Cherenkov detector radiators are identified and reviewed. Finally, current challenges and directions in the future regarding silica-based aerogels are also proposed.
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http://dx.doi.org/10.1039/d2ra01511kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092642PMC
May 2022

Bi-doped carbon dots for a stable lithium metal anode.

Chem Commun (Camb) 2022 May 12. Epub 2022 May 12.

College of Chemistry and Chemical Engineering, Central South University, Changsha, China.

Bi-doped carbon dots (Bi-CDs) with rich polar groups and good compatibility were employed as co-deposition electrolyte additives to homogenize Li flux for dendrite-free Li deposition. High coulombic efficiency (99.0%) and long-term stability (800 h) with reduced overpotential (∼15 mV) were achieved after introducing Bi-CDs in conventional electrolyte for high-performance Li-S batteries.
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http://dx.doi.org/10.1039/d2cc01334gDOI Listing
May 2022

Plasma 25-hydroxyvitamin D concentrations and incident risk of ischemic stroke in rural Chinese adults: New insight on ceiling effect.

Nutrition 2022 Feb 5;99-100:111627. Epub 2022 Feb 5.

Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, Department of Nutrition and Health, College of Food Sciences and Nutritional Engineering, China Agricultural University, Beijing, China. Electronic address:

Objective: The aim of this study was to investigate the relationship between 25-hydroxyvitamin D (25[OH]D) and ischemic stroke and its potential modifying factors in rural Chinese adults.

Methods: This nested case-control study was drawn from the H-type Hypertension and Stroke Prevention and Control Project, a community-based, prospective, observational study. Plasma 25(OH)D was measured by liquid chromatography with tandem quadrupole mass spectrometry. All stroke records came from the Chinese Center for Disease Control and Prevention. Multiple logistic regression models were used to evaluate the association between 25(OH)D and risk of ischemic stroke.

Results: We included 1079 participants with ischemic stroke and 1079 matched controls. Due to a non-linear relationship, the analyses were stratified by 25(OH)D. For those with 25(OH)D < 20 ng/mL, there was a 15% reduction in the risk of ischemic stroke for each SD increment in 25(OH)D (odds ratio, 0.85; 95% confidence interval, 0.73-0.99). Compared with the lowest-tertile group, the risk of ischemic stroke decreased by 39% (odds ratio, 0.61; 95% confidence interval, 0.41-0.89) in the highest-tertile group. Furthermore, two effect modifiers were identified: diabetes and homocysteine level. Although participants with 25(OH)D ≥ 20 ng/mL had the lowest risk of ischemic stroke overall, there was no dose-response association within that range.

Conclusions: An inverse dose-response association between 25(OH)D and incident risk of ischemic stroke in rural Chinese adults was only observed in those with 25(OH)D < 20 ng/mL, along with two effect modifiers. Higher levels of 25(OH)D did not confer additional benefit.
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http://dx.doi.org/10.1016/j.nut.2022.111627DOI Listing
February 2022

Polyphenol-driving assembly for constructing chitin-polyphenol-metal hydrogel as wound dressing.

Carbohydr Polym 2022 Aug 2;290:119444. Epub 2022 Apr 2.

College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-based Medical Materials, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, China. Electronic address:

The metal-polyphenol networks have attracted appealing attention for diverse biomedical applications due to their remarkable characteristics. Though various of metal-polyphenolic materials have been prepared, the homogeneous metal-polyphenol based hydrogel fabrication remains a challenge (e.g., quick aggregation). Herein, a facile and low-cost polyphenol-mediating non-covalent driven assembly strategy was developed for fabricating homogeneous chitin-polyphenol-metal hydrogels. Polyphenols not only noncovalently crosslinked chitin chains, but simultaneously captured metal nanomaterials from metal substrates and immobilized in chitin-polyphenol networks. A range of metal (Fe, Cu, Ti, Zn) and polyphenol (tannic acid, gallic acid, quercetin, pyrogallic acid) could be incorporated into this hydrogel framework. As a demonstration, the chitin-tannic acid-Cu hydrogel showed excellent antibacterial properties and significantly enhanced infected wound repair via promoting the cell proliferation and angiogenesis, showing the potential in wound dressing. The low cost, versatility and flexibility assembly process can be used to fabricate diverse polymer-polyphenol-metal hydrogel, thereby enabling their use in various applications.
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http://dx.doi.org/10.1016/j.carbpol.2022.119444DOI Listing
August 2022

Integration of a multichannel surface plasmon resonance sensor chip and refractive index matching film array for protein detection in human urine.

Talanta 2022 May 7;246:123533. Epub 2022 May 7.

College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address:

Most prism-based surface plasmon resonance (SPR) experiments use matching fluid with a similar refractive index to mount the chip on the optical prism. The fluidity of the matching fluid easily affects the transmission of the optical signal. In this paper, an integrated SPR sensor chip comprises a three-layer structure of flow layer, metal layer and refractive index matching layer is demonstrated to address the problems related to consistency and uniformity. The Young's modulus, array spacing, shape and other parameters of the matching film were calculated and optimized. The chip can self-adhere to the optical prism, and effectively avoids the generation of air bubbles. The refractive index detection sensitivity of the integrated SPR sensor chip was 3.4359 × 10 RIU (refractive index unit), and the chip stabilization time has been effectively shortened. The integrated SPR sensor chip was also used to detect kappa light chain protein and human serum albumin (HSA) in urine samples. The detection limit of kappa light chain protein was 0.06 μg/mL compared with 18.5 μg/mL by conventional immunoturbidimetry. The integrated SPR sensor chip based on refractive index matching film array has great potential in biomedical detection and other fields, including point-of-care testing (POCT).
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http://dx.doi.org/10.1016/j.talanta.2022.123533DOI Listing
May 2022

Erratum: Autophagy-lysosome inhibitor chloroquine prevents CTLA-4 degradation of T cells and attenuates acute rejection in murine skin and heart transplantation: Erratum.

Theranostics 2022 26;12(7):3580-3581. Epub 2022 Apr 26.

Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

[This corrects the article DOI: 10.7150/thno.43507.].
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http://dx.doi.org/10.7150/thno.73353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065174PMC
April 2022

Inhibitory effect of Lactobacillus gasseri CCFM1201 on Gardnerella vaginalis in mice with bacterial vaginosis.

Arch Microbiol 2022 May 11;204(6):315. Epub 2022 May 11.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China.

Gardnerella vaginalis is the core pathogen of bacterial vaginosis (BV), the most common vaginal infection in women. G. vaginalis exerts pathogenicity through various factors, such as biofilm formation and the local host immune response stimulation. Therefore, this study aimed to evaluate the inhibitory effect of Lactobacillus gasseri CCFM1201 on G. vaginalis using experimental BV models. We evaluated L. gasseri in vitro to inhibit pathogen biofilm formation and adhesion capacity in HeLa cells using crystal violet staining. Further in vivo studies were conducted to assess the inhibitory effects of L. gasseri CCFM1201 on BV induced by G. vaginalis. L. gasseri exhibited strain-specific adhesion and inhibition of pathogen biofilm formation in vitro. L. gasseri CCFM1201 significantly reduced G. vaginalis in mice (p < 0.05), inhibited sialidase activity, modulated tumor necrosis factor-α and interleukin-1β expression, and reduced myeloperoxidase activity (p < 0.05). Histopathological examination indicated that L. gasseri CCFM1201 improved inflammatory cell infiltration of vaginal tissue and restored its structure. Vaginal epithelial cell exfoliation, the main clinical feature of BV, was significantly improved by L. gasseri CCFM1201 intervention (p < 0.05). Thus, L. gasseri CCFM1201 is a potential candidate for treating G. vaginalis-induced vaginal diseases.
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http://dx.doi.org/10.1007/s00203-022-02896-9DOI Listing
May 2022
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