Publications by authors named "Hao Zhan"

55 Publications

Load-dependent energy dissipation induced by the tip-membrane friction on suspended 2D materials.

Phys Chem Chem Phys 2021 Sep 15;23(35):19819-19826. Epub 2021 Sep 15.

State Key Laboratory, Tsinghua University, Beijing, China.

The tip-membrane interface plays a critical role in characterizing the mechanical properties of ultrathin 2D materials by commonly employed nanoindentation based on atomic force microscopy (AFM). However, the reliability of the assumption that the tip-membrane interface remains pinned during nanoindentation remains unclear, which may introduce unignorable uncertainty in evaluating their true mechanical properties. In this work, it is reported that load-dependent frictional behavior would occur on the tip-membrane interface during nanoindentation tests on monolayer and multilayer suspended WS and graphene, and the curve hysteresis could be well explained by the stick-slip behavior. Further analyses and finite element simulations demonstrated that the frictional energy dissipation should be mainly attributed to the frictional behavior along the direction parallel to the cantilever beam. Meanwhile, the in-plane membrane stiffness was mainly responsible for the different frictional behavior on monolayer and multilayer 2D materials. Based on these analyses, some suggestions were proposed to help reduce the uncertainty when extracting the mechanical properties of 2D materials. These findings not only facilitate the deep understanding of the origin of the curve hysteresis during nanoindentation, but also help to evaluate the mechanical properties of 2D materials in a more reliable way.
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http://dx.doi.org/10.1039/d1cp02610kDOI Listing
September 2021

Quantum verification of NP problems with single photons and linear optics.

Light Sci Appl 2021 Aug 18;10(1):169. Epub 2021 Aug 18.

National Laboratory of Solid State Microstructures, Key Laboratory of Intelligent Optical Sensing and Manipulation (Ministry of Education) and College of Engineering and Applied Sciences, Nanjing University, 210093, Nanjing, China.

Quantum computing is seeking to realize hardware-optimized algorithms for application-related computational tasks. NP (nondeterministic-polynomial-time) is a complexity class containing many important but intractable problems like the satisfiability of potentially conflict constraints (SAT). According to the well-founded exponential time hypothesis, verifying an SAT instance of size n requires generally the complete solution in an O(n)-bit proof. In contrast, quantum verification algorithms, which encode the solution into quantum bits rather than classical bit strings, can perform the verification task with quadratically reduced information about the solution in [Formula: see text] qubits. Here we realize the quantum verification machine of SAT with single photons and linear optics. By using tunable optical setups, we efficiently verify satisfiable and unsatisfiable SAT instances and achieve a clear completeness-soundness gap even in the presence of experimental imperfections. The protocol requires only unentangled photons, linear operations on multiple modes and at most two-photon joint measurements. These features make the protocol suitable for photonic realization and scalable to large problem sizes with the advances in high-dimensional quantum information manipulation and large scale linear-optical systems. Our results open an essentially new route toward quantum advantages and extend the computational capability of optical quantum computing.
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http://dx.doi.org/10.1038/s41377-021-00608-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373877PMC
August 2021

Disrupted Small-world Networks are Associated with Decreased Vigilant Attention after Total Sleep Deprivation.

Neuroscience 2021 09 19;471:51-60. Epub 2021 Jul 19.

Department of Neurology, The Second Medical Center, Sleep Medicine Research Center, National Clinical Research Centre for Geriatric Diseases, Chinese PLA General Hospital, Beijing 100853, China; School of Medicine, Nankai University, Tianjin 300071, China. Electronic address:

Sleep deprivation critically affects vigilant attention. Previous neuroimaging studies have revealed altered inter-regional functional connectivity after sleep deprivation, which may disrupt topological properties of brain functional networks. However, little is known about alterations in the topology of intrinsic connectivity and its involvement in attention performance after sleep deprivation. In the current study, we investigated the topological properties of brain networks derived from resting-state functional magnetic resonance imaging of 26 healthy men in rested wakefulness (RW) state and after 36 h of total sleep deprivation (TSD). In the predefined sparsity threshold range, both global and nodal network properties were evaluated based on graph theory analysis. Vigilant attention was assessed using the psychomotor vigilance test (PVT) before and after TSD. Furthermore, Pearson's correlation analyses were conducted to explore the association between altered network properties and changed PVT performance after TSD. At the global level, the brain functional networks in the TSD state showed a significantly lower small-world coefficient than RW, with decreased global efficiency. At the nodal level, the altered regions were selectively distributed in frontoparietal networks, sensorimotor networks, temporal regions, and salience networks. More specifically, the altered clustering coefficient in the posterior superior temporal sulcus (pSTS) and insula, and altered local efficiency in pSTS were further associated with PVT performance after TSD. Our results suggest that the topological properties of brain functional networks are disrupted, and aberrant topology of temporal networks and salience networks may act as neural signatures underlying the vigilant attention impairments after TSD.
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http://dx.doi.org/10.1016/j.neuroscience.2021.07.010DOI Listing
September 2021

Altered insula-prefrontal functional connectivity correlates to decreased vigilant attention after total sleep deprivation.

Sleep Med 2021 08 5;84:187-194. Epub 2021 Jun 5.

Department of Neurology, The Second Medical Center, Sleep Medicine Research Center, National Clinical Research Centre for Geriatric Diseases, Chinese PLA General Hospital, Beijing, 100853, China; School of Medicine, Nankai University, Tianjin, 300071, China. Electronic address:

Background: Sleep deprivation can robustly affect vigilant attention. The insula is a key hub of the salience network that mediates shifting attention between endogenous and exogenous states. However, little is known regarding the involvement of insular functional connectivity in impaired vigilant attention after total sleep deprivation (TSD). The purpose of this study is to explore the alterations in insular functional connectivity and its association with vigilant attention performance following TSD.

Methods: Twenty-six adult men were enrolled in the study. Participants underwent two counterbalanced resting-state functional magnetic resonance imaging (rs-fMRI) scans, once in rested wakefulness (RW) and once after 36 h of TSD. Seed-based functional connectivity analysis was performed using rs-fMRI data for the left and right insula. The vigilant attention was measured using a psychomotor vigilance test (PVT). Furthermore, Pearson correlation analysis was conducted to investigate the relationship between altered insular functional connectivity and PVT performance.

Results: Compared to RW, enhanced functional connectivity was observed between the insula and prefrontal cortex and anterior cingulate cortex, while reduced functional connectivity was observed between the insula and temporal, parietal, and occipital regions following TSD. Moreover, altered insular functional connectivity with the prefrontal cortex, ie superior frontal gyrus and middle frontal gyrus, and inferior temporal gyrus was correlated with PVT performance after TSD.

Conclusion: Our results suggest that insular coupling with the prefrontal cortex and inferior temporal gyrus may act as neural indicators for vigilant attention impairment, which further reveals the critical role of the salience network in cognitive decline following TSD.
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http://dx.doi.org/10.1016/j.sleep.2021.05.037DOI Listing
August 2021

Fluorescent Cationic Conjugated Polymer-Based Adaptive Developing Strategy for Both Sebaceous and Blood Fingerprints.

ACS Appl Mater Interfaces 2021 Jun 3;13(23):27419-27429. Epub 2021 Jun 3.

Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.

Both latent sebaceous and blood fingerprints may provide valuable information for forensic investigation. To detect both types of fingerprints with no need to predistinguish them, a new adaptive developing strategy was proposed. A cationic conjugated polymer with poly[-(phenylene ethylene)--(thienylene ethynylene)] backbone (PPETE-NMe) was synthesized, which was dissolved in ,-dimethylformamide (DMF) to form the developing solution. Fingerprints were developed by a simple dropping and incubating process without any pre-/post-treatments. Fluorescent photographs of the developed fingerprints on various substrates demonstrated that this developing strategy was effective for both types of fingerprints on nonporous substrates. Gray value analysis further confirmed the enhancement of the legibility of the fingerprint images. The preliminary mechanism exploration suggested that certain weak interactions, such as hydrophobic interaction and electrostatic interaction, may synergistically contribute to the interaction between the polymer and fingerprint components. The molecular design of the polymer combined with an appropriate solvent endowed the developing system the adaptiveness toward different types of fingerprints. This adaptive developing strategy made the fingerprint-developing process more efficient and may be further extended to more practical application scenes.
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http://dx.doi.org/10.1021/acsami.1c04741DOI Listing
June 2021

Correlation and Survival Analysis of Distant Metastasis Site and Prognosis in Patients With Hepatocellular Carcinoma.

Front Oncol 2021 10;11:652768. Epub 2021 May 10.

School of Clinical, Graduate School of Xuzhou Medical University, Xuzhou, China.

Purpose: To investigate the prognostic factors and survival analysis of patients with hepatocellular carcinoma with distant metastasis.

Methods: The clinical data of 3,126 patients with distant metastasis of hepatocellular carcinoma from 2010 to 2015 were extracted from SEER database, and the correlation between the location of distant metastasis of hepatocellular carcinoma and prognosis was retrospectively analyzed. Patients were grouped according to different metastatic sites. The clinical characteristics of each group were compared by chi-square test, the survival curve was drawn by Kaplan-Meier method, Log-rank test was used for univariate analysis, and Cox regression for multivariate analysis. And use propensity score matching (PSM) to reduce differences in baseline characteristics.

Results: Before PSM, the prognosis of patients with hepatocellular carcinoma with lung metastasis is worse than that of patients without lung metastasis. And there was no statistically significant difference with or without bone metastases.Patients with one type of organ metastasis had better prognosis than those with multiple organ metastasis. Among patients with organ metastasis, bone metastasis has a better prognosis than patients with lung metastasis. After PSM, patients with HCC with bone metastases had a worse prognosis than those without bone metastases (<0.05). Univariate analysis showed that the degree of tumor differentiation, T stage, N stage, primary tumor and metastatic surgery, radiotherapy and chemotherapy, tumor size, single organ metastasis, the number of metastatic organs, and the combination of metastatic organs were related to the prognosis of patients with distant metastasis of hepatocellular carcinoma ( < 0.05). Multiariate analysis showed that age ≥52 years old, male, low degree of tumor differentiation, N1 stage, no primary surgery, no chemoradiotherapy, tumor size > 6cm, and multi-organ metastasis were independent influencing factors for poor prognosis in patients with metastatic hepatocellular carcinoma.

Conclusion: The lung is the most common site of distant metastasis of hepatocellular carcinoma. Single organ metastasis has better prognosis than multiple organ metastasis. Age ≥52 years old, male, low degree of tumor differentiation, N1 stage, no primary surgery, no chemoradiotherapy, tumor size > 6cm, and multi-organ metastasis were independent influencing factors for poor overall survival and cancer-specific survival prognosis in patients with metastatic hepatocellular carcinoma.
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http://dx.doi.org/10.3389/fonc.2021.652768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141638PMC
May 2021

Altered functional connectivity between the nucleus basalis of Meynert and anterior cingulate cortex is associated with declined attentional performance after total sleep deprivation.

Behav Brain Res 2021 Jul 25;409:113321. Epub 2021 Apr 25.

School of Medicine, Nankai University, Tianjin, 300071, China; Department of Neurology, The Secondary Medical Center, Sleep Medicine Research Center, National Clinical Research Centre for Geriatric Diseases, Chinese PLA General Hospital, Beijing, 100853, China. Electronic address:

Background: Sleep deprivation can markedly influence vigilant attention. The nucleus basalis of Meynert (NBM), the main source of cholinergic projections to the cortex, plays an important role in wakefulness maintenance and attention control. However, the involvement of NBM in attentional impairments after total sleep deprivation (TSD) has yet to be established. The purpose of this study is to investigate the alterations in NBM functional connectivity and its association with the attentional performance following TSD.

Methods: Thirty healthy adult males were recruited in the study. Participants underwent two resting-state functional magnetic resonance imaging (rs-fMRI) scans, once in rested wakefulness (RW) and once after 36 h of TSD. Seed-based functional connectivity analysis was performed using rs-fMRI data for the left and right NBM. The vigilant attention was measured using a psychomotor vigilance test (PVT). Furthermore, Pearson correlation analysis was conducted to investigate the relationship between altered NBM functional connectivity and changed PVT performance after TSD.

Results: Compared to RW, enhanced functional connectivity was observed between right NBM and bilateral thalamus and cingulate cortex, while reduced functional connectivity was observed between left NBM and right superior parietal lobule following TSD. Moreover, altered NBM functional connectivity with the left anterior cingulate cortex was negatively correlated with PVT performance after TSD.

Conclusion: Our results suggest that the disrupted NBM-related cholinergic circuit highlights an important role in attentional performance after TSD. The enhanced NBM functional connectivity with the anterior cingulate cortex may act as neural signatures for attentional deficits induced by sleep deprivation.
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http://dx.doi.org/10.1016/j.bbr.2021.113321DOI Listing
July 2021

Reduced Fracture Strength of 2D Materials Induced by Interlayer Friction.

Small 2021 Apr 9;17(13):e2005996. Epub 2021 Mar 9.

State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, China.

The potential applications of 2D layered materials (2DLMs) as the functional membranes in flexible electronics and nano-electromechanical systems emphasize the role of the mechanical properties of these materials. Interlayer interactions play critical roles in affecting the mechanical properties of 2DLMs, and nevertheless the understanding of their relationship remains incomplete. In the present work, it is reported that the fracture strength of few-layer (FL) WS can be weakened by the interlayer friction among individual layers with the assistance of finite element simulations and density functional theory (DFT) calculations. The reduced fracture strength can be also observed in FL WSe but with a lesser extent, which is attributed to the difference in the interlayer sliding energies of WS and WSe as confirmed by DFT calculations. Moreover, the tip-membrane friction can give rise to the underestimation of the Young's modulus except for the membrane nonlinearity. These results give deep insights into the influence of interfacial interactions on the mechanical properties of 2DLMs, and suggest that importance should be also attached to the interlayer interactions during the design of nanodevices with 2DLMs as the functional materials.
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http://dx.doi.org/10.1002/smll.202005996DOI Listing
April 2021

The Influence and Compensation Method of Eccentricity for Cylindrical Specimens in Eddy Current Displacement Measurement.

Sensors (Basel) 2020 Nov 18;20(22). Epub 2020 Nov 18.

School of Mechanical, Electrical & Information Engineering, Shandong University, Weihai 264200, China.

The eddy current displacement sensor (ECDS) is used to realize the precise detection of the rotor radial position in the magnetic suspension motor. The eccentricity between the probe axis and the measured surface normal reduces the measurement accuracy. An ECDS mathematical model is established to analyze the influence of the measured surface curvature and eccentricity on detection results. The eddy current density distribution law of the measured surface is obtained by using the finite element method (FEM). The experimental platform is set up based on the practical engineering structure, which contains two kinds structures of the single probe and the differential. The compensation method is introduced to reduce the error caused by the eccentricity. The displacement measurement error with and without compensation are tested separately. The results show that the largest full-scale error is less than 0.8% after compensation in the single probe structure, and 0.6% in the differential structure. For the engineering application, the orthogonal direction measured value is used as the eccentricity, and the compensation order of big then small is proposed. It is thus proved that the compensation method provides a guarantee for accurate feedback and control of the rotor radial position in the magnetic suspension motor system.
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http://dx.doi.org/10.3390/s20226608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698960PMC
November 2020

Discovery of small extracellular vesicle proteins from human serum for liver cirrhosis and liver cancer.

Biochimie 2020 Oct 21;177:132-141. Epub 2020 Aug 21.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:

Hepatocellular carcinoma (HCC) is a common neoplastic transformation of the hepatocytes, which has high morbidity and mortality worldwide, particularly in Eastern Asia. HCC is also developed as a consequence of chronic liver cirrhosis, and both diseases are difficult to diagnosis and differentiate. Accurate noninvasive biomarkers for HCC and cirrhosis are urgently needed. In the search for novel candidates, small extracellular vesicles (sEVs) were isolated from the serum of liver cancer patients, liver cirrhosis patients, healthy control subjects, as well as the culture media of hepatocellular carcinoma cells (HepG2) and normal hepatocyte cells (Lo2). Isolated sEVs were confirmed by size distribution analysis, morphological analysis, and surface biomarker tests. Mass spectrometry based label-free quantification revealed 61 and 63 differentially expressed proteins in the serum sEVs of liver cirrhosis patients and liver cancer patients (p < 0.05), respectively. The proteomics data of cell-derived sEVs were combined for the selection of valuable candidates. Promising proteins were further verified by immunoassay, including thrombospondin-1 (THBS1), fibulin-1(FBLN1), and fibrinogen gamma chain (FGG), which could differentiate healthy control from liver cancer or liver cirrhosis. Our findings verified the hypothesis that cancer-related proteomics signatures are present in the sEVs of patient's serum and might be monitored for the evaluation of liver cancer and liver cirrhosis.
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http://dx.doi.org/10.1016/j.biochi.2020.08.013DOI Listing
October 2020

Effects of Slip Length and Inertia on the Permeability of Fracture with Slippery Boundary Condition.

Int J Environ Res Public Health 2020 05 28;17(11). Epub 2020 May 28.

Shandong Institute of Geological Survey, Jinan 250013, China.

Although the slippery boundary condition (BC) has been validated to enhance fracture permeability (), the coupling effects of heterogeneous slippery BC and inertia on remain less understood. We used computational fluid dynamics to investigate the competing roles of slippery BC and inertial forces in controlling evolution with increasing pressure gradient by designing six cases with different slip length scenarios for a two-dimensional natural fracture. Our results suggest that pronounced inertial effects were directly related to and demonstrated by the growth of recirculation zone (RZ); this caused flow regimes transitioning from Darcy to non-Darcy and significantly reduced , with an identical tailing slope for six cases, regardless of the variability in slip lengths. Moreover, the slippery BC dominantly determine the magnitude of with orders depending on the slip length. Lastly, our study reveals that the specific evolution path for the case with a varying slip length was significantly different from other cases with a homogeneous one, thus encouraging more efforts in determining the slip length for natural fractures via experiments.
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http://dx.doi.org/10.3390/ijerph17113817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325568PMC
May 2020

Potential applications of endogenous sulfide for enhanced denitrification of low C/N domestic wastewater in anodic mixotrophic denitrification microbial fuel cell: The mechanism of electrons transfer and microbial community.

Sci Total Environ 2020 Jun 10;722:137830. Epub 2020 Mar 10.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, PR China. Electronic address:

Anodic mixotrophic denitrification microbial fuel cell (MFC) was developed for pollutants removal and electricity generation in treatment of low C/N domestic wastewater. The experimental results show that the MFC achieved up to 100% of acetate, 100% of sulfide, and more than 91% of nitrate removal efficiency in all the MFCs. Particularly, thiosulfate was generated as the main intermediate of sulfide oxidation, and the sulfate generation ratio ranged from 66.93% to 73.76%. Those electrons produced during the acetate and sulfide oxidation were mainly used for denitrification and electricity generation. The microbial community analysis revealed that heterotrophic denitrifying bacteria (HDB) and sulfide-based autotrophic denitrifying bacteria (SADB) were the dominant bacteria for pollutants removal, and those facultative autotrophic bacterium (FAB) were key functional genera for high sulfate generation under both low and high sulfide concentrations. Meanwhile, the microbial functional prediction revealed that sulfide oxidation gene of Sqr and Sox were highly expressed. Moreover, a preliminary sulfide-based autotrophic denitrification (SAD) potential estimation indicated that the sulfide generated in the WWTPs had great potential for denitrification.
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http://dx.doi.org/10.1016/j.scitotenv.2020.137830DOI Listing
June 2020

TGM3 promotes epithelial-mesenchymal transition and hepatocellular carcinogenesis and predicts poor prognosis for patients after curative resection.

Dig Liver Dis 2020 06 9;52(6):668-676. Epub 2019 Dec 9.

Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, China; Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, China. Electronic address:

Background: Prognosis of hepatocellular carcinoma (HCC) remains poor despite significant recent improvement in therapy. Recent studies have reported that transglutaminase 3 (TGM3) plays an important role in several human cancer types. However, the role of TGM3 in HCC have not been previously elucidated.

Methods: We evaluated the role of TGM3 in regulating HCC cell proliferation, migration, and invasion. We also investigated the prognostic significance of TGM3 in an HCC cohort. Finally, we explored the signalling pathways that TGM3 regulates in HCC.

Results: We identified TGM3 to be overexpressed in HCC compared to normal tissues. Higher expression of TGM3 predicts poor prognosis in HCC patients. TGM3 knockdown led to decreased HCC cell proliferation, invasion, and xenograft tumour growth. TGM3 depletion inhibited AKT, extracellular signal-regulated kinase (ERK), p65, and glycogen synthase kinase 3β (GSK3β)/β-catenin activation, but promoted levels of cleaved caspase 3. Moreover, TGM3 knockdown cells had increased E-cadherin levels and decreased vimentin levels, suggesting that TGM3 contributes to epithelial-mesenchymal transition (EMT) in HCC.

Conclusion: Our results suggest that TGM3 controls multiple oncogenic pathways in HCC, thereby contributing to increased cell proliferation and EMT, and TGM3 potentially enhances HCC metastasis. TGM3 may serve as a novel therapeutic target in HCC.
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http://dx.doi.org/10.1016/j.dld.2019.10.010DOI Listing
June 2020

Plasma hsa_circ_0027089 is a diagnostic biomarker for hepatitis B virus-related hepatocellular carcinoma.

Carcinogenesis 2020 05;41(3):296-302

Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratorsy of Carcinogenesis and Cancer Invasion of Ministry of Education, Shanghai, China.

Circular RNAs (circRNAs) have recently been identified as a new member of endogenous noncoding RNAs. CircRNAs exhibit high stability and can thus can be used as valuable biomarkers for monitoring the occurrence and development of hepatocellular carcinoma (HCC). The present study sought to explore the diagnostic significance of plasma circRNAs in hepatitis B virus (HBV)-related HCC. Plasma circRNAs from 10 patients with hepatitis B (HBV)-related HCC and 5 patients with HBV-related liver cirrhosis were investigated by microarray to screen differentially expressed circRNAs, 157 upregulated and 161 downregulated circRNAs were found. Twenty-four circRNAs were further investigated via quantitative reverse-transcriptase-polymerase chain reaction assay in a training cohort (n = 48), hsa_circ_0027089 exhibited the highest significance and further distinguished 64 HCC patients from 40 cirrhosis patients and 72 healthy participants in a validation cohort. These results indicate that plasma hsa_circ_0027089 can serve as a new marker for the diagnosis of HBV-related HCC.
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http://dx.doi.org/10.1093/carcin/bgz154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221502PMC
May 2020

Metadherin-PRMT5 complex enhances the metastasis of hepatocellular carcinoma through the WNT-β-catenin signaling pathway.

Carcinogenesis 2020 04;41(2):130-138

Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Shanghai, China.

Accumulating data suggest that metadherin (MTDH) may function as an oncogene. Our previous study showed that MTDH promotes hepatocellular carcinoma (HCC) metastasis via the epithelial-mesenchymal transition. In this study, we aim to further elucidate how MTDH promotes HCC metastasis. Using Co-immunoprecipitation (co-IP) and mass spectrometry, we found that MTDH can specifically bind to protein arginine methyltransferase 5 (PRMT5). Further functional assays revealed that PRMT5 overexpression promoted the proliferation and motility of HCC cells and that knockout of PRMT5 impeded the effect of MTDH. The immunohistochemistry assay/tissue microarray results showed that when MTDH was overexpressed in HCC cells, PRMT5 translocated from the nucleus to the cytoplasm, with the subsequent translocation of β-catenin from the cytoplasm to the nucleus and upregulation of the WNT-β-catenin signaling pathway. Further in vivo experiments suggested that PRMT5 and β-catenin played a pivotal role in MTDH-mediated HCC metastasis. We therefore concluded that the MTDH-PRMT5 complex promotes HCC metastasis by regulating the WNT-β-catenin signaling pathway.
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http://dx.doi.org/10.1093/carcin/bgz065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175245PMC
April 2020

Two-dimensional layered materials: from mechanical and coupling properties towards applications in electronics.

Nanoscale 2019 Jul 9;11(28):13181-13212. Epub 2019 Jul 9.

State Key Laboratory of Tribology, Tsinghua University, Beijing, 100084, China.

With the increasing interest in nanodevices based on two-dimensional layered materials (2DLMs) after the birth of graphene, the mechanical and coupling properties of these materials, which play an important role in determining the performance and life of nanodevices, have drawn increasingly more attention. In this review, both experimental and simulation methods investigating the mechanical properties and behaviour of 2DLMs have been summarized, which is followed by the discussion of their elastic properties and failure mechanisms. For further understanding and tuning of their mechanical properties and behaviour, the influence factors on the mechanical properties and behaviour have been taken into consideration. In addition, the coupling properties between mechanical properties and other physical properties are summarized to help set up the theoretical blocks for their novel applications. Thus, the understanding of the mechanical and coupling properties paves the way to their applications in flexible electronics and novel electronics, which is demonstrated in the last part. This review is expected to provide in-depth and comprehensive understanding of mechanical and coupling properties of 2DLMs as well as direct guidance for obtaining satisfactory nanodevices from the aspects of material selection, fabrication processes and device design.
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http://dx.doi.org/10.1039/c9nr03611cDOI Listing
July 2019

[Spatio-temporal dynamics of woody plants seed rains in broad-leaved Korean pine mixed forest in Changbai Mountains form 2006 to 2017, China.]

Ying Yong Sheng Tai Xue Bao 2019 May;30(5):1487-1493

Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shen-yang 110016, China.

Seeds are the basis for forest regeneration. To examine the composition and spatio-temporal dynamics of seed rains, a total of 150 seed traps of 0.5 m were installed in a 25 hm broad-leaved Korean pine (Pinus koraiensis) mixed forest plot in Changbai Mountains. With a total of 252 collections from May 2006 to September 2017, we collected 764299 mature and immature seeds which were belonged to 27 species, 17 genera, and 12 families. More than 90% of all collected seeds (704231 seeds) were from 13 canopy species. Seeds of four tree species, including Tilia amurensis, Fraxinus mandschurica, Acer mono, and Acer pseudo-sieboldianum could be collected every year from each trap. Mast-seeding was found in every canopy layer, but it happened one to two years earlier in the overstorey layer than midstorey and understorey layer. Almost all species produced seeds in autumn, with considerable spatiotemporal variation. Generally, the spatial variation of seeds was larger than temporal variation. Compared with annual variation coefficient of seeds in tropical forest of the Barro Colorado Island (BCI) and subtropical evergreen forest in the Gutianshan, annual variation coefficient of seeds in Changbai Mountains was higher, which supported the hypothesis that annual variation in seed rains would be lower in the tropics than that in higher latitudes.
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http://dx.doi.org/10.13287/j.1001-9332.201905.012DOI Listing
May 2019

[Effects of nitrogen and soil microbe on growth and photosynthesis of Fraxinus mandschurica seedlings.]

Ying Yong Sheng Tai Xue Bao 2019 May;30(5):1445-1462

Chinese Academy of Sciences Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

We examined the effects of nitrogen, soil microbe and their interactions on biomass allocation, growth and photosynthesis of Fraxinus mandschurica, a typical tree species in Changbai Mountain, through outdoor control experiments. In June 2017, an experiment with two-factor randomized block design was carried out. There were four treatments: control (F), nitrogen addition (FN), sterilization (FS), sterilization and nitrogen addition (FSN), six repetitive blocks, three repetitions per block, including 18 repetitions of each treatment. In mid-August 2018, we measured photosynthetic parameters and then harvested seedlings to measure biomass and growth parameters in September. The results showed that compared with F, FN significantly increased total biomass by 14%, basal diameter by 9%, chlorophyll content, net photosynthetic rate (P), stomatal conduc-tance (g), transpiration rate (T) by 75%, 318%, 231%, 227% respectively. FS significantly increased total biomass by 13%, basal diameter by 9% and chlorophyll content, P, g and T increased by 34%, 213%, 120% and 115%, respectively. FSN increased total biomass by 23%, basal diameter by 14%, chlorophyll content, P, g and T increased by 81%, 672%, 312% and 273%, respectively. Nitrogen, soil microbe and their interactions had significant effects on biomass, growth and photosynthesis of F. mandschurica seedlings. Soil microbe would regulate the response of F. mandschurica seedlings to nitrogen.
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http://dx.doi.org/10.13287/j.1001-9332.201905.018DOI Listing
May 2019

Long non-coding RNA 00607 as a tumor suppressor by modulating NF-κB p65/p53 signaling axis in hepatocellular carcinoma.

Carcinogenesis 2018 12;39(12):1438-1446

Department of Liver Surgery and Transplantation, Liver Cancer Institute, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Zhongshan Hospital of Fudan University, Shanghai, P.R. China.

Accumulating evidence suggests that long non-coding RNA (lncRNA) plays important roles in some malignant tumors. However, the mechanism underlying how lncRNA regulates hepatocellular carcinoma (HCC) process remains largely unknown. In this study, we explored the potential role of lncRNA 00607 as a novel tumor suppressor in HCC. In this study, we examined the regulation of lncRNA 00607 by the important inflammatory cytokine tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). We also determined the expression of LINC000607 in 159 HCC tumors and paired adjacent tissues. Effects of LINC000607 in HCC proliferation and apoptosis were examined in vitro in HCC cell lines and in vivo tumor xenografts. Furthermore, we also examine underlying mechanism by which lncRNA 00607 regulates NF-κB p65 and how LIN00607 exerts its tumor suppressor role in HCC. We found that lncRNA 00607 expression level is lower in HCC tumors compared with matched normal liver tissue, and its low expression predicts worse prognosis in HCC. Functionally, lncRNA 00607 overexpression leads to decreased HCC cell proliferation in vitro and in vivo, enhanced apoptosis and chemotherapeutic drug sensitivity. Mechanistically, lncRNA 00607 inhibits the p65 transcription by binding to the p65 promoter region, therefore contributing to increased p53 levels in HCC. Taken together, the findings of this study show that the TNF-α/IL-6-lncRNA 00607-NF-κB p65/p53 signaling axis represents a novel therapeutic avenue in cancer chemotherapy.
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http://dx.doi.org/10.1093/carcin/bgy113DOI Listing
December 2018

Conversion of industrial biowastes to clean solid fuels via hydrothermal carbonization (HTC): Upgrading mechanism in relation to coalification process and combustion behavior.

Bioresour Technol 2018 Nov 3;267:17-29. Epub 2018 Jul 3.

Key Laboratory of Renewable Energy, CAS, Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China.

The aim of this work was to study the correlation between dynamic mechanisms of carbon structure associated with their upgrading effects with the help of XPS, C NMR and 2D-PCIS methods. Results showed the fuel qualifies of biowastes were improved and became comparable to lignite or even bitumite after HTC. The carbon chemical bonds of -C-H and -C-O in biowaste components (mainly protein and polysaccharide) were thermally cracked and enriched in liquid phase in the form of soluble intermediates, which subsequently generated coal-like structures via cyclization as well as polymerization at higher temperatures. The further investigation on thermogravimetric analysis found that the conversion of "-C-H/C-O to aromatic -C-C/CC" was beneficial for stabilizing their combustion behavior by integrating two stages of biowastes (devolatilization stage and combustion stage) into one stage of hydrochars (combustion stage).
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http://dx.doi.org/10.1016/j.biortech.2018.07.002DOI Listing
November 2018

Clinical characteristics and prognostic significance of galectins for patients with gastric cancer: A meta-analysis.

Int J Surg 2018 Aug 27;56:242-249. Epub 2018 Jun 27.

The First Department of General Surgery, The Second Hospital of Lanzhou University, Lanzhou, 730000, Gansu, China. Electronic address:

Objective: To explore the relationships between the expression level of different galectins and its prognostic value for patients with gastric cancer.

Methods: The PubMed, EMbase, the Cochrane Library and Web of Science databases were systematically searched. All the eligible studies were included according to the inclusion and exclusion criteria. All the relevant data was extracted by two independent researchers. The quality assessment was conducted according to the evaluation of the quality of prognosis study which published by Harden in 2006. The STATA 12.0 software was used to perform a meta-analysis.

Results: All of 8 retrospective case-controlled studies involving 2093 patients with gastric cancer were included in this study. The results of meta-analysis presented that the elevated galectin-1 which is related to the poor overall survival (HR = 1.85, 95% CI: 1.33-2.58; P < 0.001) may predicted a larger tumor size (OR = 2.20, 95% CI: 1.35-3.35; P = 0.001) and was positively associated with the higher expression of VEGF (OR = 1.44, 95% CI: 1.14-1.82; P = 0.002). Moreover, the decreased galectin-3 (HR = 0.49, 95% CI: 0.36-0.67; P < 0.001), galectin-8 (HR = 0.49, 95% CI: 0.36-0.67; P < 0.001) and galectin-9 (HR = 0.78, 95% CI: 0.66-0.92; P = 0.003) were also significantly associated with poorer prognosis. Our meta-analysis also showed that lower expression of galectin-3 was also related to lymphatic vessel invasion (OR = 0.48, 95% CI: 0.26-0.89; P = 0.018), worse TNM stages (OR = 0.47, 95% CI: 0.32-0.40; P < 0.001), deeper invasive depth (OR = 0.33, 95% CI: 0.21-0.51; P < 0.001) and poorer differentiation grade (OR = 0.10, 95% CI: 0.04-0.25; P < 0.001).

Conclusions: High expression of galectin-1 or low expression of galectin-3, -8 and -9 were significantly related to a poorer prognosis for patients with gastric cancer. The expression level of galectins was associated with clinical characteristics and were potential independent prognostic predictor for GC patients.
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http://dx.doi.org/10.1016/j.ijsu.2018.06.033DOI Listing
August 2018

microRNA-501-3p suppresses metastasis and progression of hepatocellular carcinoma through targeting LIN7A.

Cell Death Dis 2018 05 1;9(5):535. Epub 2018 May 1.

Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University; Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, 200032, Shanghai, China.

Increasing numbers of evidences have demonstrated that microRNAs (miRNAs) are implicated in metastasis and progression of hepatocellular carcinoma (HCC). However, their detailed expression levels and actual functions in HCCs have not been fully clarified yet. Results from our recent study revealed that some miRNAs were particularly related to metastasis of HCCs. As one of these newly found miRNAs, miR-501-3p showed to highly involve into metastatic process of HCCs. Here we reported that the expression of miR-501-3p was decreased in both metastatic HCC cell lines and tissue samples from HCC patients with recurrence and metastasis. Downregulation of miR-501-3p correlated with tumor progression and poor prognosis in the HCC patients. Results of functional analyses revealed that overexpression of miR-501-3p in HCCLM3 cancer cells inhibited their proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), while miR-501-3p loss in PLC/PRF/5 cancer cells facilitated all these cellular activities. In addition, Lin-7 homolog A (LIN7A) was directly targeted by miR-501-3p to mediate the suppression effects on metastasis in HCC cells. miR-501-3p suppresses metastasis and progression of HCCs through targeting LIN7A. This finding suggests that miR-501-3p could be used as a potential prognostic predictor as well as a potential therapeutic tool for HCC therapies.
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http://dx.doi.org/10.1038/s41419-018-0577-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945677PMC
May 2018

[Factors influencing tree radial growth of three common species in broad-leaved Korean pine mixed forests in Changbai Mountains, China].

Ying Yong Sheng Tai Xue Bao 2017 May;28(5):1407-1413

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

To understand the factors influencing tree radial growth, we analyzed the seasonal dynamics of tree growth of 3 common species (Pinus koraiensis, Tilia amurensis, Quercus mongolica), compared interspecific growth rates and explored the effects of size, neighborhood competition, soil and topography based on five years dendrometer bands monitoring data of the 3 common species in a broad-leaved Korean pine (P. koraiensis) mixed forest plot in Changbai Mountain, Northeast China. The results showed that the growth dynamics of 3 species were consistent. Trees began to grow in late May, thrived in July, grew at declining rates from late August and stopped growing in late October. Annual relative growth rates were significantly different among the species. Q. mongolica tended to grow faster than the other two species, and the differences of growth rates among the 3 species were especially large for small and medium trees. Tree growth rates of P. koraiensis and Q. mongolica were strongly decreased by neighborhood competition, while tree growth rate of T. amurensis was significantly related to tree size, soil and topography.
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http://dx.doi.org/10.13287/j.1001-9332.201705.031DOI Listing
May 2017

A systematic review and network meta-analysis protocol of neoadjuvant treatments for patients with gastric cancer.

Medicine (Baltimore) 2018 Apr;97(15):e0392

Department of First General Surgery, Lanzhou University Second Hospital Department of Endocrinology, Lanzhou University First Hospital, Lanzhou, China.

Background: National Comprehensive Cancer Network (NCCN) guidelines recommend surgery, chemotherapy, and radiation therapy for gastric cancer patients. Neoadjuvant treatments as the administration of therapeutic agents before a main treatment gained in more and more attention. However, the role of neoadjuvant treatments is still controversial. The main aim of this systematic review and network meta-analysis is to assess the relative efficacy of different neoadjuvant treatment regimens for gastric cancer using network meta-analysis method.

Methods: We will search 5 electronic databases to identify randomized controlled trials (RCTs) and non-RCTs compared the efficacy differences of surgery alone (S), preoperative chemotherapy follow by surgery (CTS), preoperative radiotherapy follow by surgery (RTS), and preoperative chemoradiotherapy follow by surgery (CRTS) for patients with gastric cancer. The risk of bias tool from the Cochrane Handbook version 5.1.0 will be used to assess the risk of bias of RCTs, and the risk of bias in nonrandomized studies of interventions (ROBINS-I) for non-RCTs. Data will be analyzed using R-3.4.1 software.

Results And Conclusion: The results of present network meta-analysis will estimate the relative efficacy among all interventions and rank the interventions even if head-to-head comparisons are lacking and will provide more evidence for clinicians, researchers, and patients in the management of gastric cancer.Protocol registration number: CRD42017074956.
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http://dx.doi.org/10.1097/MD.0000000000010392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908618PMC
April 2018

Relevance between chemical structure and pyrolysis behavior of palm kernel shell lignin.

Sci Total Environ 2018 Aug 28;633:785-795. Epub 2018 Mar 28.

CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.

Palm kernel shell (PKS) lignin obtained by enzymatic/mild acid hydrolysis (EMAL) was thoroughly elucidated by FTIR (fourier transform infrared), C-H 2D-NMR (nuclear magnetic resonance), quantitative P NMR combined with DFRC (derivatization followed by reductive cleavage), and Py-GC/MS (pyrolysis-gas chromatography/mass spectrometry) with and without TMAH (tetramethylammonium hydroxide). Pyrolysis behavior was then characterized by TG-FTIR-MS (thermo-gravimetric-FTIR-mass spectrometry) and Py-GC/MS. The PKS lignin is demonstrated to be a p-hydroxyphenyl-guaiacyl-syringyl (H-G-S) lignin with abundances of p-hydrobenzoates and low S/G ratio of 0.15. 2D-NMR indicated that the main substructures are β-O-4-ethers (~85%), and P NMR/DFRC quantified the total β-O-4 content of 2295μmol/g. Py-GC/MS with and without TMAH confirmed that phenol mainly originated from p-hydroxybenzoates units. Thermal-stability, evolution behavior of typical volatiles, and selectivity of phenolic compounds (H-, G-, S-, C-type) during PKS lignin pyrolysis were explored. Relationship between chemical structure and pyrolysis behavior are also obtained. This work will provide a deep insight to the effective utilization of PKS.
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http://dx.doi.org/10.1016/j.scitotenv.2018.03.238DOI Listing
August 2018

Denitrification and desulphurization of industrial biowastes via hydrothermal modification.

Bioresour Technol 2018 Apr 13;254:121-129. Epub 2018 Jan 13.

Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, China; CAS Key Laboratory of Renewable Energy, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, China.

In attempt to decrease NO and SO emission from thermochemical utilization, three industrial biowastes (penicillin mycelia waste, sewage sludge and peat waste) contained high nitrogen (N) and sulfur (S) were chosen to investigate the denitrification and desulphurization of hydrothermal modification. The results demonstrated that hydrothermal modification destroyed the structure of N- and S-containing components, thereby altering their existed conformations. Inorganic-N (N-IN) and most of amino-N/polyamide-N (N-A) were enriched by liquid phase in the forms of NH-N and soluble organic-N (Org-N), respectively; subsequently, Org-N could further decompose to NH-N at higher temperature. Residual N in hydrochars was converted from N-A to heterocyclic-N (pyrrolic-N, pyridinic-N and quaternary-N) via hydrolysis and cyclization. Similarly, over 60% of S was remove form biowastes at 240 °C. In solid phase, part of organic-S was altered to thiophenes-S after modified, while the remainder was transformed to inorganic-S; but the variation of inorganic-S in hydrochars strongly affected by its specific species.
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http://dx.doi.org/10.1016/j.biortech.2018.01.061DOI Listing
April 2018

Whole-Exome Sequencing-Based Mutational Profiling of Hepatitis B Virus-Related Early-Stage Hepatocellular Carcinoma.

Gastroenterol Res Pract 2017 29;2017:2029315. Epub 2017 Nov 29.

Liver Cancer Institute, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Hepatocellular carcinoma (HCC) ranks as the third leading cause of cancer-related mortality in China with increasing incidence. This study is designed to explore early genetic changes implicated in HCC tumorigenesis and progression by whole-exome sequencing.

Methods: We firstly sequenced the whole exomes of 5 paired hepatitis B virus-related early-stage HCC and peripheral blood samples, followed by gene ontological analysis and pathway analysis of the single-nucleotide variants discovered. Then, the mutations of high frequency were further confirmed by Sanger sequencing.

Results: We identified a mutational signature of dominant T:A>A:T transversion in early HCC and significantly enriched pathways including ECM-receptor interaction, axon guidance, and focal adhesion and enriched biological processes containing cell adhesion, axon guidance, and regulation of pH. Eight genes, including MUC16, UNC79, USH2A, DNAH17, PTPN13, TENM4, PCLO, and PDE1C, were frequently mutated.

Conclusions: This study reveals a mutational profile and a distinct mutation signature of T:A>A:T transversion in early-stage HCC with HBV infection, which will enrich our understanding of genetic characteristics of the early-stage HCC.
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http://dx.doi.org/10.1155/2017/2029315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733245PMC
November 2017

Downregulation of MAGE family member H1 enhances hepatocellular carcinoma progression and serves as a biomarker for patient prognosis.

Future Oncol 2018 May 10;14(12):1177-1186. Epub 2018 Jan 10.

Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis & Cancer Invasion, Fudan University, Ministry of Education, Shanghai 200032, PR China.

Aim: The MAGE family member H1 (MAGEH1) belongs to melanoma-associated antigen (MAGE) superfamily. The role of MAGEH1 in hepatocellular carcinoma (HCC) is largely undefined.

Materials & Methods: We used quantitative reverse transcription PCR and immunohistochemistry to detect MAGEH1 expression in HCC tissues. CCK-8 assay, wound healing migration assay and Transwell Matrigel invasion assay were used to measure HCC cell proliferation, migration and invasion ability.

Results:  MAGEH1 expression was downregulated in HCC tumor tissues compared with adjacent normal liver tissues and in samples from patients with tumor recurrence. MAGEH1 reduced HCC cell proliferation, migration and invasion ability. Low MAGEH1 expression was significantly correlated with poor prognosis in HCC patients.

Conclusion:  MAGEH1 may serve as a potential biomarker and a new prognostic factor for HCC.
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http://dx.doi.org/10.2217/fon-2017-0672DOI Listing
May 2018

The transformation pathways of nitrogen in sewage sludge during hydrothermal treatment.

Bioresour Technol 2017 Dec 1;245(Pt A):463-470. Epub 2017 Sep 1.

Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, China; CAS Key Laboratory of Renewable Energy, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, China.

Hydrothermal treatment (HT) has been proved as a significant pretreatment in decreasing emissions of NO pollutants from thermochemical utilization of sewage sludge (SS) derived solid fuel. This study aims to investigate the denitrification of HT and the redistribution of nitrogen (N) in different products so as to speculate the comprehensive pathway of N transformation during hydrothermal process. Results found that only 20% of N remained in hydrochar, whereas the rest of N (nearly 80%) was transformed into other phase. A majority of amino-N in SS was enriched in liquid phase in the form of Org-N at first, then further decomposed to NH-N. The remaining amino-N converted to pyrrole-N, pyridine-N and quaternary-N as temperature progresses. Meanwhile, amine-N derived from protein-N formed heterocyclic-N in oil phase via Diels-Alder reaction. NH, the major nitrogenous gas, was dissolved in liquid as NH-N immediately after producing, but increased with prolonged reaction time.
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http://dx.doi.org/10.1016/j.biortech.2017.08.195DOI Listing
December 2017

[Small intestinal cavernous hemangioma causing chronic hemorrhage: a case report].

Nan Fang Yi Ke Da Xue Xue Bao 2017 Jul;37(7):866-868

Department of General Surgery, Lanzhou University Second Hospital, Lanzhou 730030, China.E-mail:

Small intestinal hemangioma is a rare condition that can be divided histologically into capillary, cavernous or mixed types, among which the cavernous type is the most common. Here we report a case of small intestinal cavernous hemangioma with chronic hemorrhage in 44-year-old man. The patient complained of weakness and dizziness for 2 years that aggravated 1 month before admission accompanied by intermittent melena. Laboratory tests suggest severe anemia, and computed tomography, gastroscopy and colonoscopy all revealed signs of anemia. Capsule endoscopy detected small intestinal erosions, bleeding lesions and prominent neoplasms. An exploratory laparotomy was performed, in which the segment of the jejunum with lesions was resected. Pathological examination of the resected jejunum identified the neoplasm as cavernous hemangioma of the small intestine, which was the cause of severe anemia.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765523PMC
July 2017
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