Publications by authors named "Hao Yang"

1,286 Publications

  • Page 1 of 1

Effects of influent immigration and environmental factors on bacterial assembly of activated sludge microbial communities.

Environ Res 2021 Nov 26:112426. Epub 2021 Nov 26.

School of Environmental and Material Engineering, Yantai University, Yantai, 264005, China. Electronic address:

The functional mechanism of microbial assembly of activated sludge (AS) in urban wastewater treatment plants (UWTPs) remains unclear. A comprehensive quantitative evaluation of the contribution of influent immigration and environmental factors to AS community composition requires investigation. In this study, the microbial characteristics of six full-scale UWTPs with different influent compositions and environmental factors (altitude, temperature, dissolved oxygen (DO), pH, chemical oxygen demand (COD), total nitrogen (TN), ammonia nitrogen (NH-N), and total phosphorus (TP)) were analyzed to determine the main forces affecting the bacterial assembly of AS microbial communities. Abundant and core taxa were screened out based on the abundance and frequency of operational taxonomic units (OTUs) occurrence in all samples. Abundant OTUs (18.7% occurrence) accounted for 87.7% of the total 16S rRNA sequences, while rare OTUs (71.7% occurrence) accounted for only 7.8% of the total 16S rRNA sequences. A total of 135 OTUs were identified as core taxa, accounting for 14.6-26.2% of the total reads, of which 83 OTUs belonged to abundant taxa. The richness and uniformity of the influent community were significantly lower than those of the AS system. The community composition in influent varied from that in AS. Moreover, about 89.7% (86.5% of 16S rRNA sequences) OTUs in AS samples showed positive growth rates, indicating that immigration of influent communities had a limited effect on the microbial composition of AS. Redundancy analysis (RDA) combined with co-occurrence network showed that the bacterial assembly of microbial communities was significantly correlated with altitude, pH, and TN (P < 0.05), and these three parameters could explain 23.3%, 21.1%, and 17.7% of the bacterial assembly of AS microbial communities in UWTPs, respectively.
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http://dx.doi.org/10.1016/j.envres.2021.112426DOI Listing
November 2021

[Application of CRISPR/Cas-based biosensors for detecting nucleic acid of pathogens].

Sheng Wu Gong Cheng Xue Bao 2021 Nov;37(11):3890-3904

School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats -associated protein (CRISPR/Cas) has been developed as a precise, efficient, affordable and sensitive nucleic acid detection tool due to its efficient targeted binding ability and programmability. At present, biosensors based on CRISPR-Cas system have shown excellent performance in the detection of nucleic acid of pathogens, which has attracted widespread attention, and is expected to replace the conventional detection methods. This review summarizes the latest research progress of biosensors based on CRISPR/Cas system for detecting nucleic acid of pathogens.
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http://dx.doi.org/10.13345/j.cjb.200679DOI Listing
November 2021

Circ_MUC16 attenuates the effects of Propofol to promote the aggressive behaviors of ovarian cancer by mediating the miR-1182/S100B signaling pathway.

BMC Anesthesiol 2021 Nov 27;21(1):297. Epub 2021 Nov 27.

Department of Anesthesiology, The Second People's Hospital of Kunming, Kunming College, No. 871, Longquan Road, Kunming, 650200, Yunnan, China.

Background: Propofol is commonly used for anesthesia during surgery and has been demonstrated to inhibit cancer development, which is shown to be associated with deregulation of non-coding RNAs (ncRNAs). The objective of this study was to explore the role of circular RNA mucin 16 (circ_MUC16) in Propofol-mediated inhibition of ovarian cancer.

Methods: The expression of circ_MUC16, microRNA-1182 (miR-1182) and S100 calcium-binding protein B (S100B) mRNA was measured by quantitative real-time polymerase chain reaction (qPCR). The expression of S100B protein was checked by western blot. Cell proliferation was assessed by 3-(4, 5-di methyl thiazol-2-yl)-2, 5-di phenyl tetrazolium bromide (MTT) assay and colony formation assay. Glycolysis metabolism was assessed by glucose consumption, lactate production and ATP level. Cell migration and cell invasion were assessed by transwell assay. Cell migration was also assessed by wound healing assay. Animal study was conducted in nude mice to determine the role of circ_MUC16 in vivo. The relationship between miR-1182 and circ_MUC16 or S100B was validated by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.

Results: Propofol inhibited ovarian cancer cell proliferation, glycolysis metabolism, migration and invasion, which were partly recovered by circ_MUC16 overexpression. Circ_MUC16 was downregulated in Propofol-treated ovarian cancer cells. Besides, circ_MUC16 knockdown enhanced the effects of Propofol to further inhibit tumor growth in vivo. MiR-1182 was a target of circ_MUC16, and circ_MUC16 knockdown-inhibited cell proliferation, glycolysis metabolism, migration and invasion were partly restored by miR-1182 inhibition. In addition, S100B was a target of miR-1182, and miR-1182-suppressed cell proliferation, glycolysis metabolism, migration and invasion were partly restored by S100B overexpression.

Conclusion: Circ_MUC16 overexpression alleviated the effects of Propofol to promote the aggressive behaviors of ovarian cancer by targeting the miR-1182/S100B network.
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http://dx.doi.org/10.1186/s12871-021-01517-0DOI Listing
November 2021

Visible-Light Photosynthesis of CHF /CClF /CBrF -Substituted Ring-fused Quinazolinones in Dimethyl Carbonate.

Chem Asian J 2021 Nov 26. Epub 2021 Nov 26.

School of Chemistry and Chemical Engineering, University of South China, Hengyang, 421001, P. R. China.

With eco-friendly and sustainable CO -derived dimethyl carbonate as the sole solvent, the visible-light-induced cascade radical reactions have been established as a green and efficient tool for constructing various CHF /CClF /CBrF -substituted ring-fused quinazolinones.
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http://dx.doi.org/10.1002/asia.202101139DOI Listing
November 2021

Construction of a Multifunctional Nano-Scale Metal-Organic Framework-Based Drug Delivery System for Targeted Cancer Therapy.

Pharmaceutics 2021 Nov 17;13(11). Epub 2021 Nov 17.

School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 102488, China.

The antitumor activity of triptolide (TP) has received widespread attention, although its toxicity severely limits its clinical application. Therefore, the design of a targeted drug delivery system (TDDS) has important application prospects in tumor treatment. Metal-organic frameworks (MOFs), with high drug-carrying capacity and good biocompatibility, have aroused widespread interest for drug delivery systems. Herein, folic acid (FA) and 5-carboxylic acid fluorescein (5-FAM) were used to modify Fe-MIL-101 to construct a functionalized nano-platform (5-FAM/FA/[email protected]) for the targeted delivery of the anti-tumor drug triptolide and realize in vivo fluorescence imaging. Compared with Fe-MIL-101, functionalized nanoparticles not only showed better targeted therapy efficiency, but also reduced the systemic toxicity of triptolide. In addition, the modification of 5-FAM facilitated fluorescence imaging of the tumor site and realized the construction of an integrated nano-platform for fluorescence imaging and treatment. Both in vitro and in vivo studies of functionalized nanoparticles have demonstrated excellent fluorescence imaging and synergistic targeting anticancer activity with negligible systemic toxicity. The development of functional nano-platform provides new ideas for the design of MOF-based multifunctional nano-drug delivery system, which can be used for precise treatment of tumor.
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http://dx.doi.org/10.3390/pharmaceutics13111945DOI Listing
November 2021

Feedback Graph Convolutional Network for Skeleton-based Action Recognition.

IEEE Trans Image Process 2021 Nov 24;PP. Epub 2021 Nov 24.

Skeleton-based action recognition has attracted considerable attention since the skeleton data is more robust to the dynamic circumstances and complicated backgrounds than other modalities. Recently, many researchers have used the Graph Convolutional Network (GCN) to model spatial-temporal features of skeleton sequences by an end-to-end optimization. However, conventional GCNs are feedforward networks for which it is impossible for the shallower layers to access semantic information in the high-level layers. In this paper, we propose a novel network, named Feedback Graph Convolutional Network (FGCN). This is the first work that introduces a feedback mechanism into GCNs for action recognition. Compared with conventional GCNs, FGCN has the following advantages: (1) A multi-stage temporal sampling strategy is designed to extract spatial-temporal features for action recognition in a coarse to fine process; (2) A Feedback Graph Convolutional Block (FGCB) is proposed to introduce dense feedback connections into the GCNs. It transmits the high-level semantic features to the shallower layers and conveys temporal information stage by stage to model video level spatial-temporal features for action recognition; (3) The FGCN model provides predictions on-the-fly. In the early stages, its predictions are relatively coarse. These coarse predictions are treated as priors to guide the feature learning in later stages, to obtain more accurate predictions. Extensive experiments on three datasets, NTU-RGB+D, NTU-RGB+D120 and Northwestern-UCLA, demonstrate that the proposed FGCN is effective for action recognition. It achieves the state-of-the-art performance on all three datasets.
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http://dx.doi.org/10.1109/TIP.2021.3129117DOI Listing
November 2021

The exposure of OPFRs in fish from aquaculture area: Backward tracing of the ecological risk regulation.

Environ Pollut 2021 Nov 20;293:118550. Epub 2021 Nov 20.

MOE Key Laboratory of Resources and Environmental Systems Optimization, North China Electric Power University, Beijing, 102206, China. Electronic address:

In this study, we backward traced and controlled the pollution of organophosphorus flame retardants (OPFRs) in aquaculture areas from the standpoints of terminal treatment, migration and transformation resistance, and source molecular substitution technology. A regulatory plan to considerably reduce the combined biotoxicity of fish exposed to OPFRs in aquaculture areas and significantly improves the biodegradation of sewage treatment and the efficiency of soil plant-microorganism combined remediation was formulated. Environmentally friendly alternatives of OPFRs were designed. The supplementation scheme of aquatic feed significantly alleviates the toxicity risk of fish exposure to OPFRs in aquafarm (reduced by 121.02%). The regulatory scheme of external stimulus to enhance the biodegradation of OPFRs in wastewater treatment process included an HO concentration of 400 mg/L, voltage gradient of 1.5 V/m, and pH of 6.5 can improve the degradation capacity of OPFRs molecules by 88.86%. The degradation of OPFRs can be enhanced by plant-microorganism combined remediation (up to 98.64%) by growing plants whose primary function is phytoextraction in soils dominated by Sphingopyxis sp. and Rhodococcus sp. A 3D-QSAR pharmacophore model based on apoptosis toxicity, mitochondrial dysfunction, oxidative stress response, reproductive, neurotoxicity, gill-inhalation combined toxicity of fish exposed to OPFRs in aquafarm was fabricated. The recommended aquatic feed scheme and the control scheme of enhanced degradation of OPFRs by sewage treatment and soil environment had better applicability for the new-designed OPFRs substitution molecules (the maximum combined toxicity/degradation is reduced/increased by 75.46% and 63.24%, respectively). In this paper, a technical scheme of OPFRs terminal treatment, process regulation, and source control was applied as a cradle-to-grave approach to reduce the ecological toxicity risk of fish exposed to OPFRs in aquaculture areas providing theoretical support for the realization of OPFRs environmental pollution control.
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http://dx.doi.org/10.1016/j.envpol.2021.118550DOI Listing
November 2021

High-efficient subwavelength-scale optofluidic waveguides with tapered microstructured optical fibers.

Opt Express 2021 Nov;29(23):38068-38081

Microstructured optical fibers (MOFs) have attracted intensive research interest in fiber-based optofluidics owing to their ability to have high-efficient light-microfluid interactions over a long distance. However, there lacks an exquisite design guidance for the utilization of MOFs in subwavelength-scale optofluidics. Here we propose a tapered hollow-core MOF structure with both light and fluid confined inside the central hole and investigate its optofluidic guiding properties by varying the diameter using the full vector finite element method. The basic optical modal properties, the effective sensitivity, and the nonlinearity characteristics are studied. Our miniature optofluidic waveguide achieves a maximum fraction of power inside the core at 99.7%, an ultra-small effective mode area of 0.38 µm, an ultra-low confinement loss, and a controllable group velocity dispersion. It can serve as a promising platform in the subwavelength-scale optical devices for optical sensing and nonlinear optics.
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http://dx.doi.org/10.1364/OE.443846DOI Listing
November 2021

Phenanthrocarbazole-Based Dopant-Free Hole-Transport Polymer with Noncovalently Conformational Locking for Efficient Perovskite Solar Cells.

Angew Chem Int Ed Engl 2021 Nov 22. Epub 2021 Nov 22.

Westlake University, School of Science, CHINA.

Adequate hole mobility is the prerequisite for dopant-free polymeric hole-transport materials (HTMs). Constraining configurational variation of polymer chains to afford a rigid and planar backbone can reduce unfavorable reorganization energy and improve hole mobility. Herein, a noncovalently conformational locking via S--O secondary interaction is exploited in a phenanthrocarbazole ( PC ) based polymeric HTM, PC6 , to fix molecular geometries and significantly reduce reorganization energy. Systematic study from structurally explicit repeats to targeted polymers reveals that the broad and planar backbone of PC remarkably enhances π-π stacking of adjacent polymers, facilitating intermolecular charge transfer greatly. The inserted "Lewis soft" oxygen atoms passivate the trap sites efficiently at perovskite/HTM interface and further depressing interfacial recombination. Consequently, PSC employing PC6 as dopant-free HTM offers an excellent power conversion efficiency of 22.2% and significantly improved longevity, rendering it as one of the best PSCs based on dopant-free HTMs.
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http://dx.doi.org/10.1002/anie.202114341DOI Listing
November 2021

The camphor tree genome enhances the understanding of magnoliid evolution.

J Genet Genomics 2021 Nov 16. Epub 2021 Nov 16.

College of Forestry, Fujian Agriculture and Forestry, Fuzhou 350002, China; Fujian Colleges and Universities Engineering Research Institute of Conservation and Utilization of Natural Bioresources, Fujian Agriculture and Forestry, Fuzhou 350002, China; Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:

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http://dx.doi.org/10.1016/j.jgg.2021.11.001DOI Listing
November 2021

Integrin-β6 Serves as a Potential Prognostic Serum Biomarker for Gastric Cancer.

Front Oncol 2021 2;11:770997. Epub 2021 Nov 2.

Department of Gastrointestinal Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.

Discovering novel biomarkers that easily accessed is a key step towards the personalized medicine approach for gastric cancer patients. Integrin-β6 (ITGB6) is a subtype of integrin that is exclusively expressed on the surface of epithelial cells and is up-regulated in various tumors. In the present study, a retrospective cohort with 135 gastric cancer patients and a prospective cohort with 34 gastric cancer patients were constructed, ITGB6 expression were detected in both the serum specimens and the tissue specimens. Detailed clinicopathological parameters as well as patients' survival were recorded. A nomogram including ITGB6 expression was also constructed and validated to predict the prognosis of gastric cancer patients. Results showed that serum ITGB6 expression was obviously increased and associated with tumor stage in gastric cancer patients, serum ITGB6 expression was relatively high in patients with liver metastasis. High ITGB6 expression indicated a poor prognosis, and nomogram including serum ITGB6 expression could predict the prognosis of gastric cancer patients effectively. Moreover, serum ITGB6 expression was associated with ITGB6 expression in tumor tissues. Furthermore, combined serum ITGB6 and CEA levels contributed to the risk stratification and prognostic prediction for gastric cancer patients. In addition, the serum expression of ITGB6 decreased significantly after radical surgery, and a new rise in serum ITGB6 expression indicated tumor recurrence or progression. The present study identified a novel serum biomarker for the risk stratification, prognostic prediction and surveillance of gastric cancer patients.
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http://dx.doi.org/10.3389/fonc.2021.770997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593195PMC
November 2021

LncSNHG3 promotes hepatocellular carcinoma epithelial mesenchymal transition progression through the miR-152-3p/JAK1 pathway.

Genes Genomics 2021 Nov 15. Epub 2021 Nov 15.

Department of Radiation Oncology, Inner Mongolia Cancer Hospital and Affiliated People's Hospital of Inner Mongolia Medical University, 010020, Huhhot, China.

Background: The dysregulation of LncRNAs is related to the malignant progression of many cancers.

Objective: The study aimed to investigate the expression and the biological role of LncSNHG3 in hepatocellular carcinoma (HCC).

Methods: The TCGA data of the LncSNHG3 in HCC were analyzed. The expression in HCC cell lines was detected by qRT-PCR. Proliferation, migration, and invasion of HepG2 and Huh7 were examined by cell counting kit-8, colony formation, transwell assays, and wound healing assays. At the same time, the interactions among LncSNHG3, miR-152-3p, and JAK1 were confirmed by dual-luciferase reporter assay, RNA immunoprecipitation, subcellular distribution. Xenograft tumor-bearing mice models were used to measure the effect of LncSNHG3 on the growth of HCC in vivo. The apoptosis and epithelial mesenchymal transition (EMT)-associated proteins were checked by WB and IHC.

Results: LncSNHG3 was overexpressed in HCC tissues and cell lines. In addition, it is correlated with the tumor stage and survival time of HCC patients. Down-regulated LncSNHG3 could significantly suppress the EMT progression of HCC in vivo and in vitro. LncSNHG3 could promote the JAK1 expression by sponging miR-152-3p.

Conclusions: LncSNHG3 acted as an oncogene and promoted the EMT procession in HCC by binding miR-152-3p and promoting JAK1 expression. Predictably, LncSNHG3 was used as a potential marker and will be used as a novel therapy target for HCC in the future.
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http://dx.doi.org/10.1007/s13258-021-01185-xDOI Listing
November 2021

capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China.

Anim Dis 2021 2;1(1):26. Epub 2021 Nov 2.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

is a leading cause of respiratory disorders in pigs. This study was designed to understand the genotypical and antimicrobial resistant characteristics of from pigs in China. To achieve this, we briefly investigated 158 isolates from pigs with respiratory disorders in China between 2019 and 2020. Genotyping through multiplex PCR assays assigned these 158 isolates into capsular genotypes A (60.13%, 95/158), D (35.44%, 56/158), F (4.43%, 7/158), and/or lipopolysaccharide (LPS) genotypes L3 (28.48%, 45/158) and L6 (66.46%, 105/158). In addition, eight isolates (5.06%, 8/158) were found to be nontypable using the LPS genotyping method. When combining the capsular genotypes and the LPS genotypes, D: L6 (34.81%, 55/158) and A: L6 (31.65%, 50/158) were the predominant genotypes, followed by A: L3 (24.05%, 38/158). PCR detection of virulence factor-encoding genes showed that over 80% of the isolates were positive for , , , , , , , , , , , , , and , suggesting the presence of these genes were broad characteristics of . We also found approximately 63.92% (101/158), 51.27% (81/158), 8.86% (14/158), 7.59% (12/158), 3.16% (5/158), 0.63% (1/158), and 0.63% (1/158) of the isolates grew well in media with the presence of colistin (4 μg/mL), tetracycline (16 μg/mL), tigecycline (1 μg/mL), ampicillin (32 μg/mL), chloramphenicol (32 μg/mL), cefepime (16 μg/mL), and ciprofloxacin (1 μg/mL), respectively. This study contributes to the understanding of genotypes and antimicrobial resistance profile of currently circulation in pigs of China.

Supplementary Information: The online version contains supplementary material available at 10.1186/s44149-021-00031-7.
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http://dx.doi.org/10.1186/s44149-021-00031-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8561366PMC
November 2021

Post-angiography Retention of the Contrast Agent in the Left Atrial Appendage Is Associated With Risk of Cardioembolic Stroke in Patients With Atrial Fibrillation: A Retrospective Study.

Front Cardiovasc Med 2021 28;8:753949. Epub 2021 Oct 28.

Department of Cardiology, The First Affiliated Hospital (Yijishan Hospital) of Wannan Medical College, Wuhu, China.

Atrial fibrillation (AF) represents an important risk factor for cardioembolic stroke, and most atrial thrombi originate from the left atrial appendage (LAA). Although the CHADS-VASc score is widely used to estimate the risk of cardioembolic stroke in AF patients, yet greatly affected by many factors. This study was undertaken to determine the association between contrast agent retention in LAA after LAA angiography and risks of cardioembolic stroke in patients with AF. This is a retrospective study. The demographic and clinical data of AF patients undergone left atrial appendage occlusion (LAAO) with or without catheter radiofrequency ablation were retrospectively analyzed. The patients were classified into either stroke or non-stroke group by the history with cardioembolic stroke or transient ischemic attack (TIA). Sixty-two consecutive patients undergone LAAO were finally included, in whom 31 AF patients had a history of cardioembolic stroke or TIA (one TIA), and significantly higher CHADS-VASc score (4.2 ± 1.4 vs. 3.3 ± 1.3; = 0.006) as well as incidence of contrast agent retention in LAA ( = 20 vs. = 7; = 0.001) compared to the patients in non-stroke group. In addition, the relative proportion of distinctive morphological types of LAA was significantly different between groups ( < 0.001). Multivariate logistic regression analysis showed that higher CHADS-VASc scores ( = 1.7, 95% : 1.0-3.0, = 0.046) and LAA contrast agent retention ( = 5.1, 95% : 1.1-23.9, = 0.002) were associated with increased risks of cardioembolic stroke. The patients with Windsock type LAA ( = 7.8, 95% : 1.1-57.2, = 0.044) and Cauliflower LAA ( = 20.2, 95% : 3.2-125.5, = 0.001) were more prone to cardioembolic stroke compared to those with Chicken Wing type LAA. Left atrial appendage contrast agent retention after LAA angiography is associated with the risks of cardioembolic stroke in patients with AF, and cardioembolic stroke is more seen in AF patients with Windsock or Cauliflower type LAA.
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http://dx.doi.org/10.3389/fcvm.2021.753949DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581209PMC
October 2021

Identification and Characterization of Resistance Genes in .

Front Plant Sci 2021 29;12:758559. Epub 2021 Oct 29.

Provincial Key Laboratory for Plant Genetics and Breeding, Chengdu, China.

is an important multiuse perennial plant that often suffers attacks from various pathogens due to its long growth cycle, seriously affecting its commercial value. The absence of research on the resistance () genes of has greatly limited progress in the breeding of resistant varieties. Genes encoding proteins containing nucleotide binding sites (NBSs) and C-terminal leucine-rich repeats (LRRs), the largest family of plant resistance () genes, are vital for plant disease resistance. A comprehensive genome-wide analysis showed that there were only 73 genes in the genome, including three main subfamilies (50 ()- (), 19 ()- () and four ()-- () genes). Additionally, 64 mapped candidates were unevenly distributed on 14 chromosomes, most of which were assigned to the chromosome ends; 41 of these genes were located in clusters, and the remaining 23 genes were singletons. Both the and were further divided into four subgroups, and the had fewer exons than the . Structurally, all eight previously reported conserved motifs were identified in the NBS domains, and both their order and their amino acid sequences exhibited high conservation. Evolutionarily, tandem and dispersed duplications were shown to be the two main forces responsible for expansion, producing 33 and 29 genes, respectively. A transcriptome analysis of three fruit tissues at four developmental stages showed that genes were generally expressed at low levels, while a few of these genes showed relatively high expression during later development in rind tissues. Overall, this research is the first to identify and characterize genes and is valuable for both the development of new resistant cultivars and the study of molecular mechanisms of resistance.
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http://dx.doi.org/10.3389/fpls.2021.758559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585750PMC
October 2021

Quantitative Study on Solubility Parameters and Related Thermodynamic Parameters of PVA with Different Alcoholysis Degrees.

Polymers (Basel) 2021 Oct 31;13(21). Epub 2021 Oct 31.

Key Laboratory of Bio-Based Material Science and Technology of Ministry of Education, College of Materials Science and Engineering, Northeast Forestry University, Harbin 150040, China.

In recent years, inverse gas chromatography (IGC) and molecular dynamics simulation methods have been used to characterize the solubility parameters and surface parameters of polymers, which can provide quantitative reference for the further study of the surface and interface compatibility of polymer components in the future. In this paper, the solubility parameters and surface parameters of two kinds of common alcoholysis, PVA and PVA, are studied by using the IGC method. The accuracy of the solubility parameters obtained by the IGC experiment is verified by molecular dynamics simulation. On the basis of this, the influence of repeated units of polyvinyl alcohol (PVA) on solubility parameters is studied, so as to determine the appropriate chain length of the PVA for simulation verification calculation. The results show that the solubility parameters are not much different when the PVA chain length is 30 and above; the numerical trends of the solubility parameters of PVA and PVA at room temperature are the same as the results of molecular dynamics simulation; the dispersive surface energy γsd and the specific surface energy γssp are scattered with the temperature distribution and have a small dependence on temperature. On the whole, the surface energy of PVA with a higher alcoholysis degree is higher than that of PVA with a lower alcoholysis degree. The surface specific adsorption free energy (ΔGsp) indicates that both PVA and PVA are amphoteric meta-acid materials, and the acidity of PVA is stronger.
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http://dx.doi.org/10.3390/polym13213778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586924PMC
October 2021

Vagal response during circumferential pulmonary vein isolation decreases the recurrence of atrial fibrillation in the short-term in patients with paroxysmal atrial fibrillation: A prospective, observational study.

J Electrocardiol 2021 Nov-Dec;69:145-150. Epub 2021 Oct 30.

Department of Intensive Care Medicine, The First Affifiliated Hospital (Yijishan Hospital) of Wannan Medical College, Wuhu, Anhui 241001, China. Electronic address:

Background: Vagal responses (VRs) are often seen in patients undergoing circumferential pulmonary vein isolation (CPVI). The possible mechanism of VR is that CPVI creates a coincidental modification of the cardiac ganglionated plexi (GP).

Aim: To investigate whether the presence of VR during CPVI impacts post-ablation recurrence in patients with paroxysmal atrial fibrillation (AF).

Methods: A total of 112 consecutive patients with symptomatic paroxysmal AF who underwent CPVI for the first time from October 1, 2017 to April 30, 2019 were prospectively enrolled, of which two were lost the follow-up. Patients were divided into two groups based on whether VRs were experienced during CPVI. Electrophysiological parameters, including atrial effective refractory period (AERP) and mean heart rate (MHR), were measured before and post-ablation. The patients were then followed up for 12 months.

Results: The 71 patients who had experienced VRs during CPVI were assigned to group B, and the remaing 39 patients who did not experience VR during CPVI were assigned to group A. The MHR (79.6 ± 8.3 vs 70.4 ± 7.8 b/min; p ≤ 0.001) was significantly higher; and the AERP (244 ± 22 vs 215 ± 27 ms; p ≤ 0.001) was prolonged in group B compared to respective pre-ablation values. There were no significant changes in the MHR (69.5 ± 7.9 vs 69.7 ± 8.7 b/min; p = 0.541) and AERP (224 ± 28 vs 225 ± 33 ms; p = 0.542) in group A. During the first four months of follow-up after ablation, the MHR gradually slowed down to pre-procedural levels in group B. The recurrence of AF (6/71 vs 7/39; p = 0.023) significantly decreased in group B relative to group A during the first 6 months after ablation, but there was no significant difference (14/71 vs 9/39; p = 0.598) at the end of the 12-month follow-up period.

Conclusion: Patients with paroxysmal AF who develop VRs during CPVI might have a decreased recurrence of AF and accelerated MHR in the short-term.
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http://dx.doi.org/10.1016/j.jelectrocard.2021.10.007DOI Listing
October 2021

The Melastoma dodecandrum genome and the evolution of Myrtales.

J Genet Genomics 2021 Oct 28. Epub 2021 Oct 28.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Art & Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Innovation and Application Engineering Technology Research Center of Ornamental Plant Germplasm Resources in Fujian Province, Fuzhou 350002, China. Electronic address:

Melastomataceae have abundant morphological diversity with high economic and ornamental merit in Myrtales. The phylogenetic position of Myrtales is still contested. Here, we report the first chromosome-level genome assembly of Melastoma dodecandrum in Melastomataceae. The assembled genome size was 299.81 Mb with a contig N50 value of 3.00 Mb. Genome evolution analysis indicated that M. dodecandrum, Eucalyptus grandis and Punica granatum were clustered into a clade of Myrtales and formed a sister group with the ancestor of fabids and malvids. We found that M. dodecandrum experienced four whole-genome polyploidization events: the ancient event was shared with most eudicots, one event was shared with Myrtales, and the other two events were unique to M. dodecandrum. Moreover, we identified MADS-box genes and found that the AP1-like genes expanded, and AP3-like genes might have undergone subfunctionalization. We found that the SUAR63-like genes and AG-like genes showed different expression patterns in stamens, which may be associated with heteranthery. In addition, we found that LAZY1-like genes were involved in the negative regulation of stem branching development, which may be related to its creeping features. Our study sheds new light on the evolution of Melastomataceae and Myrtales, which provides a comprehensive genetic resource for future research.
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http://dx.doi.org/10.1016/j.jgg.2021.10.004DOI Listing
October 2021

Diagnosis and staging of diffuse large B-cell lymphoma using label-free surface-enhanced Raman spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 1;267(Pt 1):120571. Epub 2021 Nov 1.

Department of Hematology, Harbin Medical University Cancer Hospital, 150 Haping Road, 150081 Harbin, China.

Non-invasive diagnosis and staging of diffuse large B-cell lymphoma (DLBCL) were achieved using label-free surface-enhanced Raman spectroscopy (SERS). SERS spectra were measured for serum samples of DLBCL patients at different progressive stages and healthy controls (HCs), using colloidal silver nano-particles (AgNPs) as the substrate. Differences in the spectral intensities of Raman peaks were observed between the DLBCL and HC groups, and a close correlation between the spectral intensities of Raman peaks with the progressive stages of the cancer was obtained, demonstrating the possibility of diagnosis and staging of the disease using the serum SERS spectra. Multivariate analysis methods, including principal component analysis (PCA), linear discriminant analysis (LDA), support vector machine (SVM) classifier, and k-nearest neighbors (kNN) classifier, were used to build the diagnosis and staging models for DLBCL. Leave-one-out cross-validation was used to evaluate the performances of the models. The kNN model achieved the best performances for both diagnosis and staging of DLBCL: for the diagnosis analysis, the accuracy, sensitivity, and specificity were 87.3%, 0.921, and 0.809, respectively; for the staging analysis between the early (Stage I & II) and the late (Stage III & IV) stages, the accuracy was 90.6%, and the sensitivity values for the early and the late stages were 0.947 and 0.800, respectively. The label-free serum SERS in combination with multivariate analysis could serve as a potential technique for non-invasive diagnosis and staging of DLBCL.
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http://dx.doi.org/10.1016/j.saa.2021.120571DOI Listing
November 2021

A versatile platform for the tumor-targeted delivery of immune checkpoint-blocking immunoglobin G.

J Control Release 2021 Nov 6;340:243-258. Epub 2021 Nov 6.

Key Lab of Transplant Engineering and Immunology, MOH, West China Hospital, Sichuan University, Chengdu 610041, China; Sichuan Provincial Engineering Laboratory of Pathology in Clinical Application, West China Hospital, Sichuan University, Chengdu 610041, China; Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address:

Immunotherapies based on immune checkpoint-blocking antibodies have been considered the most attractive cancer treatments in recent years. However, the systemic administration of immune checkpoint-blocking antibodies is limited by low response rates and high risk of inducing immune-related adverse events (irAEs), which might be overcome by the tumor-targeted delivery of these antibodies. To achieve tumor-targeted delivery, immune checkpoint-blocking antibodies are usually modified with tumor-homing ligands through difficult genetic fusion or chemical conjugation. As most immune checkpoint-blocking antibodies are immunoglobin G (IgG) antibodies, we hypothesize that these IgG antibodies might be noncovalently modified with a tumor-homing ligand fused to an IgG-binding domain (IgBD). To test this hypothesis, the tumor-homing Z affibody, which targets platelet-derived growth factor receptor β (PDGFRβ), was fused to the Fab-selective IgBD in a trimeric format. After mixing Z fused to the IgBD with immune checkpoint-blocking IgG against programmed death-ligand 1 (αPD-L1), a novel homogenous complex was formed, indicating that αPD-L1 had been successfully modified with Z fused to the IgBD. Z-modified αPD-L1 bound to both PDGFRβ and PD-L1, thus leading to greater tumor uptake and antitumor effects in mice bearing PDGFRβPD-L1 tumor grafts. In addition, due to the broad spectrum of IgBD for IgG, immune checkpoint-blocking IgG antibodies against cytotoxic T-lymphocyte-associated protein 4 (αCTLA-4) and signal regulatory protein alpha (αSIRPα) were also modified with Z fused to the IgBD. These results demonstrated that a tumor-homing ligand fused to the IgBD might be developed as a versatile platform for the modification of immune checkpoint-blocking IgG antibodies to achieve tumor-targeted delivery.
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http://dx.doi.org/10.1016/j.jconrel.2021.11.003DOI Listing
November 2021

Systems analysis of plasma IgG intact -glycopeptides from patients with chronic kidney diseases EThcD-sceHCD-MS/MS.

Analyst 2021 Nov 22;146(23):7274-7283. Epub 2021 Nov 22.

NHC Key Laboratory of Transplant Engineering and Immunology, Institutes for Systems Genetics; National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.

Immunoglobulin G (IgG) molecules modulate an immune response. However, site-specific -glycosylation signatures of plasma IgG in patients with chronic kidney disease (CKD) remain unclear. This study aimed to propose a novel method to explore the -glycosylation pattern of IgG and to compare it with reported methods. We separated human plasma IgG from 58 healthy controls (HC) and 111 patients with CKD. Purified IgG molecules were digested by trypsin. Tryptic peptides without enrichment of intact -glycopeptides were analyzed using a combination of electron-transfer/higher-energy collisional dissociation (EThcD) and stepped collision energy/higher-energy collisional dissociation (sceHCD) mass spectrometry (EThcD-sceHCD-MS/MS). This resulted in higher spectral quality, more informative fragment ions, higher Byonic score, and nearly twice the depth of intact -glycopeptide identification than sceHCD or EThcD alone. Site-specific -glycosylation mapping revealed that intact -glycopeptides were differentially expressed in HC and CKD patients; thus, it can be a diagnostic tool. This study provides a method for the determination of glycosylation patterns in CKD and a framework for understanding the role of IgG in the pathophysiology of CKD. Data are available ProteomeXchange with identifier PXD027174.
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http://dx.doi.org/10.1039/d1an01657aDOI Listing
November 2021

LncRNA OTUD6B-AS1 Induces Cisplatin Resistance in Cervical Cancer Cells Through Up-Regulating Cyclin D2 miR-206.

Front Oncol 2021 22;11:777220. Epub 2021 Oct 22.

Department of Radiation Oncology, Inner Mongolia Cancer Hospital and Affiliated People's Hospital of Inner Mongolia Medical University, Huhhot, China.

Cervical cancer is one of the most common gynecological cancers. Cisplatin resistance remains a major hurdle in the successful treatment of cervical cancer. Aberrant expression of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are implicated in cisplatin resistance. However, the regulatory functions of lncRNAs and miRNAs in cervical cancer cisplatin resistance and the underlying mechanisms are still elusive. Our qRT-PCR assays verified that miR-206 levels were down-regulated in cisplatin-resistant cervical cancer cells. The introduction of miR-206 sensitized cisplatin-resistant cervical cancer cells to cisplatin. Our qRT-PCR and luciferase reporter assays showed that Cyclin D2 () was the direct target for miR-206 in cervical cancer cells. The cisplatin-resistant cervical cancer cells expressed higher expression than the parental cells, whereas inhibition of CCND2 could sensitize the resistant cells to cisplatin treatment. Furthermore, we demonstrated that lncRNA OTUD6B-AS1 was up-regulated in cisplatin-resistant cervical cancer cells, and knocking down OTUD6B-AS1 expression induced re-acquirement of chemosensitivity to cisplatin in cervical cancer cells. We also showed that OTUD6B-AS1 up-regulated the expression of CCND2 by sponging miR-206. Low miR-206 and high OTUD6B-AS1 expression were associated with significantly poorer overall survival. Taken together, these results suggest that OTUD6B-AS1-mediated down-regulation of miR-206 increases CCND2 expression, leading to cisplatin resistance. Modulation of these molecules may be a therapeutic approach for cisplatin-resistant cervical cancer.
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http://dx.doi.org/10.3389/fonc.2021.777220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569895PMC
October 2021

Sequential Analysis of the N/O-Glycosylation of Heavily Glycosylated HIV-1 gp120 Using EThcD-sceHCD-MS/MS.

Front Immunol 2021 21;12:755568. Epub 2021 Oct 21.

National Health Commission (NHC) Key Laboratory of Transplant Engineering and Immunology, Institutes for Systems Genetics, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, China.

Deciphering the glycosylation of the viral envelope (Env) glycoprotein is critical for evaluating viral escape from the host's immune response and developing vaccines and antiviral drugs. However, it is still challenging to precisely decode the site-specific glycosylation characteristics of the highly glycosylated Env proteins, although glycoproteomics have made significant advances in mass spectrometry techniques and data analysis tools. Here, we present a hybrid dissociation technique, EThcD-sceHCD, by combining electron transfer/higher-energy collisional dissociation (EThcD) and stepped collision energy/higher-energy collisional dissociation (sceHCD) into a sequential glycoproteomic workflow. Following this scheme, we characterized site-specific N/O-glycosylation of the human immunodeficiency virus type 1 (HIV-1) Env protein gp120. The EThcD-sceHCD method increased the number of identified glycopeptides when compared with EThcD, while producing more comprehensive fragment ions than sceHCD for site-specific glycosylation analysis, especially for accurate O-glycosite assignment. Finally, eighteen N-glycosites and five O-glycosites with attached glycans were assigned unambiguously from heavily glycosylated gp120. These results indicate that our workflow can achieve improved performance for analysis of the N/O-glycosylation of a highly glycosylated protein containing numerous potential glycosites in one process. Knowledge of the glycosylation landscape of the Env glycoprotein will be useful for understanding of HIV-1 infection and development of vaccines and drugs.
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http://dx.doi.org/10.3389/fimmu.2021.755568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567067PMC
October 2021

Biomechanical properties of erythrocytes circulating in artificial hearts measured by dielectrophoretic method.

J Biomech 2021 Oct 23;129:110822. Epub 2021 Oct 23.

Robotics and Microsystems Center, School of Mechanical and Electric Engineering, Soochow University, China. Electronic address:

Blood damage is recognized as one of the major problems caused by non-physiological shear force induced by artificial hearts. At present, the generally accepted manifestation of mechanical blood damage is the amount of free hemoglobin released into the blood. However, there is little research on the changes of blood cell state after circulating in artificial hearts at the single-cell level. It is well known that the mechanical properties of cells are of enormous relevance in the regulation of cellular physiological and pathological processes. In this regard, it is highly needed to study the mechanical properties of blood cells affected by non-physiological shear force. In this paper, a dielectrophoresis-based method of measuring the mechanical properties of erythrocytes circulating in artificial hearts was proposed, which was quantified with some crucial parameters such as strain, elongation index (EI), and Young's modulus. Experimental results indicated that with the increase of the working time of artificial hearts, the deformability of erythrocytes decreased, the stiffness substantially increased, and the mechanical stability decreased, particularly at long exposure times. The proposed method provides a deep insight into the mechanism of subhemolytic damage at the single-cell level and has a great potential to serve as a new tool for in vitro evaluation of potential blood damage in artificial hearts.
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http://dx.doi.org/10.1016/j.jbiomech.2021.110822DOI Listing
October 2021

Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 increases cellular proliferation and colony formation capacity in lung cancer via activation of E2F transcription factor 1.

Oncol Lett 2021 Dec 26;22(6):851. Epub 2021 Oct 26.

Department of Pulmonary and Critical Care Medicine, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530021, P.R. China.

Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1) is an enzyme that catalyzes the hydroxylation of lysyl residues in collagen-like peptides, and is responsible for the stability of intermolecular crosslinks. High PLOD1 mRNA levels have been determined to be prognostically significant in numerous human malignancies. The objective of the present study was to elucidate the pathological mechanism of PLOD1 in lung cancer. The expression status and prognostic value of PLOD1 in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSA) were investigated using Gene Expression Profiling Interactive Analysis (GEPIA). Cell Counting Kit 8 and colony formation assays were performed to assess the impact of PLOD1 depletion and overexpression on the proliferation and colony formation abilities of the A549 lung cancer cell line. Luciferase reporter assays were used to clarify whether E2F transcription factor 1 (E2F1) was a downstream target of PLOD1 in lung cancer. Finally, the correlations between PLOD1 expression and a typical central downstream effector molecule of E2F1 signaling were determined using cBioportal. The GEPIA datasets revealed that PLOD1 mRNA levels were upregulated in LUAD and LUSC samples. Furthermore, the overexpression of PLOD1 promoted cancer cell proliferation and colony formation , while PLOD1-knockout produced the opposite effect. Notably, PLOD1 markedly induced the transcriptional activity of E2F1. Additionally, the expression of PLOD1 was significantly correlated with that of H2A histone family member X. In conclusion, the findings of the present study indicate that PLOD1 promoted lung cancer through E2F1 activation, and proposed a rationale for targeting the PLOD1/E2F1 axis to treat lung cancer.
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http://dx.doi.org/10.3892/ol.2021.13112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8561618PMC
December 2021

Intervention Effect of Long-Term Aerobic Training on Anxiety, Depression, and Sleep Quality of Middle School Students With Depression After COVID-19.

Front Psychiatry 2021 18;12:720833. Epub 2021 Oct 18.

College of Physical Education, Guizhou Normal University, Guiyang, China.

To explore the effects of using RPE exercise intensity monitoring methods and 12-week mid- and low-intensity team aerobic training on anxiety, depression and sleep quality of depressed middle school students after the COVID-19 epidemic. All study participants were all from a boarding middle school in Chongqing, China. All study participants were screened by the self-rating depression scale and reached the diagnostic criteria for depression. The study subjects were divided into a control group ( = 35) and an exercise group ( = 34). The exercise group performed 30-min night aerobic running four times a week. Use the Borg 6-20 rating of perceived exertion scale (RPE) as a monitoring tool for exercise intensity, and control the exercise intensity at RPE = 11-14. And the control group studied and lived normally. The experiment lasted 12 weeks in total. After the experiment, there were 34 people in the control group and 23 people in the exercise group. The subjects' anxiety, depression and Pittsburgh sleep quality were scored before and after the experiment. After intervention, the depression index ( < 0.01) of the exercise group was significantly lower than that of the control group. Using the RPE exercise intensity monitoring method for 12 weeks of mid- and low-intensity team aerobic training can improve the depressive symptoms of depressed middle school students, and it is beneficial to improve the students' mental health.
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http://dx.doi.org/10.3389/fpsyt.2021.720833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558258PMC
October 2021

Genome-wide investigation of the GRAS transcription factor family in foxtail millet (Setaria italica L.).

BMC Plant Biol 2021 Nov 3;21(1):508. Epub 2021 Nov 3.

College of Agriculture, Guizhou University, Guiyang, 550025, People's Republic of China.

Background: GRAS transcription factors perform indispensable functions in various biological processes, such as plant growth, fruit development, and biotic and abiotic stress responses. The development of whole-genome sequencing has allowed the GRAS gene family to be identified and characterized in many species. However, thorough in-depth identification or systematic analysis of GRAS family genes in foxtail millet has not been conducted.

Results: In this study, 57 GRAS genes of foxtail millet (SiGRASs) were identified and renamed according to the chromosomal distribution of the SiGRAS genes. Based on the number of conserved domains and gene structure, the SiGRAS genes were divided into 13 subfamilies via phylogenetic tree analysis. The GRAS genes were unevenly distributed on nine chromosomes, and members of the same subfamily had similar gene structures and motif compositions. Genetic structure analysis showed that most SiGRAS genes lacked introns. Some SiGRAS genes were derived from gene duplication events, and segmental duplications may have contributed more to GRAS gene family expansion than tandem duplications. Quantitative polymerase chain reaction showed significant differences in the expression of SiGRAS genes in different tissues and stages of fruits development, which indicated the complexity of the physiological functions of SiGRAS. In addition, exogenous paclobutrazol treatment significantly altered the transcription levels of DELLA subfamily members, downregulated the gibberellin content, and decreased the plant height of foxtail millet, while it increased the fruit weight. In addition, SiGRAS13 and SiGRAS25 may have the potential for genetic improvement and functional gene research in foxtail millet.

Conclusions: Collectively, this study will be helpful for further analysing the biological function of SiGRAS. Our results may contribute to improving the genetic breeding of foxtail millet.
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http://dx.doi.org/10.1186/s12870-021-03277-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565077PMC
November 2021

Flexible electrodes with high areal capacity based on electrospun fiber mats.

Nanoscale 2021 Nov 18;13(44):18391-18409. Epub 2021 Nov 18.

State Key Laboratory of Heavy Oil Processing, Institute of New Energy, College of Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China.

The ever-growing portable, flexible, and wearable devices impose new requirements from power sources. In contrast to gravitational metrics, areal metrics are more reliable performance indicators of energy storage systems for portable and wearable devices. For energy storage devices with high areal metrics, a high mass loading of the active species is generally required, which imposes formidable challenges on the current electrode fabrication technology. In this regard, integrated electrodes made by electrospinning technology have attracted increasing attention due to their high controllability, excellent mechanical strength, and flexibility. In addition, electrospun electrodes avoid the use of current collectors, conductive additives, and polymer binders, which can essentially increase the content of the active species in the electrodes as well as reduce the unnecessary physically contacted interfaces. In this review, the electrospinning technology for fabricating flexible and high areal capacity electrodes is first highlighted by comparing with the typical methods for this purpose. Then, the principles of electrospinning technology and the recent progress of electrospun electrodes with high areal capacity and flexibility are elaborately discussed. Finally, we address the future perspectives for the construction of high areal capacity electrodes using electrospinning technology to meet the increasing demands of flexible energy storage systems.
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http://dx.doi.org/10.1039/d1nr05681fDOI Listing
November 2021

Dietary resveratrol alleviated lipopolysaccharide-induced ileitis through Nrf2 and NF-κB signalling pathways in ducks (Anas platyrhynchos).

J Anim Physiol Anim Nutr (Berl) 2021 Nov 2. Epub 2021 Nov 2.

Institute of Animal Nutrition, Northeast Agricultural University, Xiangfang District, Harbin, China.

Gram-negative bacteria contamination of feed can occur at all the stage of feed production, storage, transportation and utilization. Lipopolysaccharide (LPS) is a major toxic metabolite of Gram-negative bacteria. The aim of this study was to explore the effect of dietary resveratrol on the duck ileitis caused by LPS and its optimum addition level in diet. The results showed that LPS-induced duck ileitis with the destruction of intestinal structure, oxidative stress, mitochondrial dysfunction, inflammatory response and permeability alteration. Dietary resveratrol alleviated LPS-induced intestinal dysfunction and the increase of intestinal permeability by linearly increasing mRNA levels of tight junction protein genes (Claudin-1, Occludin-1, ZO-1) (p < 0.05) and protein expression of Claudin-1 (p < 0.01). In addition, dietary resveratrol improved the antioxidant capacity of duck ileum by reducing the production of MDA and increasing the activity of T-SOD (p < 0.01) and CAT. Lipopolysaccharide increased Keap1 at mRNA and protein level (p < 0.01) and decreased the protein level of Nrf2 (p < 0.05). Dietary resveratrol significantly downregulated expression of Keap1 and upregulated expression of Nrf2 in duck (p < 0.05). Dietary resveratrol suppressed the TLR4/NF-κB signalling pathway and the expression of its downstream genes including IKK, TXNIP, NLRP3, Caspase-1, IL-6 and IL-18. Meanwhile, the levels of inflammatory cytokines (IL-6, IL-18 and TNF-α) showed a linearly decrease (p < 0.01) with increasing dietary resveratrol level. These results demonstrated that resveratrol alleviated the LPS-induced acute ileitis of duck through Nrf2 and NF-κB signalling pathways, and the dietary resveratrol of 500 mg/kg is more efficiently.
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http://dx.doi.org/10.1111/jpn.13657DOI Listing
November 2021
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