Publications by authors named "Hao Wu"

3,458 Publications

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Poor Bone Quality, Multilevel Surgery, and Narrow and Tall Cages Are Associated with Intraoperative Endplate Injuries and Late-onset Cage Subsidence in Lateral Lumbar Interbody Fusion: A Systematic Review.

Clin Orthop Relat Res 2021 Jul 29. Epub 2021 Jul 29.

Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong SAR, China.

Background: A major complication of lateral lumbar interbody fusion (LLIF) is cage subsidence, which may lead to clinical problems, including loss of disc height correction, altered spinal alignment, recurrent pain, and vertebral body fracture. A thorough review of the current knowledge about the risk factors for the two types of cage subsidence after LLIF-intraoperative endplate injury and late-onset cage subsidence-could bring attention to well-established risk factors for clinical consideration while identifying any incompletely characterized factors that require further research to clarify.

Questions/purposes: We performed a systematic review to answer the following questions: (1) Are bone quality and surrogates for bone quality, such as patient age and sex, associated with an increased likelihood of cage subsidence? (2) Are implant-related factors associated with an increased likelihood of cage subsidence?

Methods: Two independent reviewers comprehensively searched Medline, Embase, Cochrane Library, PubMed, and Web of Science from 1997 to 2020 to identify all potential risk factors for cage subsidence after LLIF. Discrepancies were settled through discussion during full-text screening. Search terms included "lateral" AND "interbody fusion" AND "subsidence" OR "settling" OR "endplate injury" OR "endplate violation" WITHOUT "cervical" OR "transforaminal" OR "biomechanical." Eligible studies were retrospective or prospective comparative studies, randomized controlled trials, and case series with sample sizes of 10 patients or more reporting risk factors for cage subsidence or endplate injury after LLIF. Studies that involved cervical interbody fusions and biomechanical and cadaveric experiments were excluded. The Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach was used to assess the studies' quality of evidence. The initial database review found 400 articles. Thirty-four articles with moderate- to very-low-quality evidence met the inclusion criteria for analysis. A total of 3233 patients (58% [1860] of whom were female) were included in this review. Two types of cage subsidence were reviewed: late-onset cage subsidence, which occurs gradually postoperatively, and intraoperative endplate injury, which is derived from iatrogenic endplate violation during endplate preparation or cage insertion. Among 20 studies with moderate quality of evidence according to the GRADE criteria, eight studies reported risk factors for cage subsidence related to bone mineral density and its surrogates and 12 studies focused on risk factors regarding implant factors, including cage dimension, cage material, construct length, and supplementary instrumentation.

Results: Patients with a dual x-ray absorptiometry T-score of -1.0 or less, age older than 65 years, and female sex were considered to have a high risk of both types of cage subsidence. Regarding cage size, cage width ≥ 22 mm helped to avoid late-onset cage subsidence, and cage height ≤ 11 mm was recommended by some studies to avoid intraoperative endplate injuries. Studies recommended that multilevel LLIF should be conducted with extra caution because of a high risk of losing the effect of indirect decompression. Studies found that standalone LLIF might be sufficient for patients without osteoporosis or obesity, and supplementary instrumentation should be considered to maintain the postoperative disc height and prevent subsidence progression in patients with multiple risk factors. The effect of the bone graft, cage material, endplate condition, and supplementary instrumentation on cage subsidence remained vague or controversial.

Conclusion: Patients with poor bone density, patients who are older than 65 years, and female patients should be counseled about their high risk of developing cage subsidence. Surgeons should avoid narrow cages when performing LLIF to minimize the risk of late-onset cage subsidence, while being cautious of an aggressive attempt to restore disc height with a tall cage as it may lead to intraoperative endplate injury. For multilevel constructs, direct decompression approaches, such as posterior and transforaminal LIF, should be considered before LLIF, since the effect of indirect decompression may be difficult to maintain in multilevel LLIF because of high risks of cage subsidence. The effect of the cage material and supplementary instrumentation require stronger evidence from prospectively designed studies with larger sample size that randomly assign patients to polyetheretherketone (PEEK) or titanium cages and different fixation types. Future research on intraoperative endplate injuries should focus on the specific timing of when endplate violation occurs with the help of intraoperative imaging so that attempts can be made to minimize its occurrence.

Level Of Evidence: Level IV, therapeutic study.
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http://dx.doi.org/10.1097/CORR.0000000000001915DOI Listing
July 2021

Comparative transcriptomics and network analysis define gene coexpression modules that control maize aleurone development and auxin signaling.

Plant Genome 2021 Jul 29:e20126. Epub 2021 Jul 29.

Dep. of Genetics, Development & Cell Biology, IA State Univ., Ames, IA, 50011, USA.

The naked endosperm1 (nkd1), naked endosperm2 (nkd2), and thick aleurone1 (thk1) genes are important regulators of maize (Zea mays L.) endosperm development. Double mutants of nkd1 and nkd2 (nkd1,2) show multiple aleurone (AL) cell layers with disrupted AL cell differentiation, whereas mutants of thk1 cause multiple cell layers of fully differentiated AL cells. Here, we conducted a comparative analysis of nkd1,2 and thk1 mutant endosperm transcriptomes to study how these factors regulate gene networks to control AL layer specification and cell differentiation. Weighted gene coexpression network analysis was incorporated with published laser capture microdissected transcriptome datasets to identify a coexpression module associated with AL development. In this module, both Nkd1,2+ and Thk1+ appear to regulate cell cycle and division, whereas Nkd1,2+, but not Thk1+, regulate auxin signaling. Further investigation of nkd1,2 differentially expressed genes combined with published putative targets of auxin response factors (ARFs) identified 61 AL-preferential genes that may be directly activated by NKD-modulated ARFs. All 61 genes were upregulated in nkd1,2 mutant and the enriched Gene Ontology terms suggested that they are associated with hormone crosstalk, lipid metabolism, and developmental growth. Expression of a transgenic DR5-red fluorescent protein auxin reporter was significantly higher in nkd1,2 mutant endosperm than in wild type, supporting the prediction that Nkd1,2+ negatively regulate auxin signaling in developing AL. Overall, these results suggest that Nkd1,2+ and Thk1+ may normally restrict AL development to a single cell layer by limiting cell division, and that Nkd1,2+ restrict auxin signaling in the AL to maintain normal cell patterning and differentiation processes.
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http://dx.doi.org/10.1002/tpg2.20126DOI Listing
July 2021

Differences in Retinal and Choroidal Vasculature and Perfusion Related to Axial Length in Pediatric Anisomyopes.

Invest Ophthalmol Vis Sci 2021 Jul;62(9):40

Eye Hospital and School of Optometry and Ophthalmology, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Purpose: The purpose of this study was to evaluate the interocular differences in choroidal vasculature, choriocapillaris perfusion, and retinal microvascular network, and to explore their associations with interocular asymmetry in axial lengths (ALs) in children with anisomyopia.

Methods: Refractive error, AL, and other biometric parameters were measured in 70 children with anisomyopia. Using optical coherence tomography (OCT) and OCT-angiography, we measured the submacular choroidal thickness (ChT), total choroidal area (TCA), luminal area (LA), stromal area (SA), choroidal vascularity index (CVI), choriocapillaris flow deficit (CcFD), retinal vessel density (VD), and foveal avascular zone (FAZ) area.

Results: The mean interocular differences in spherical equivalent refraction and AL were -2.26 ± 0.94 diopters and 0.95 ± 0.46 mm, respectively. Submacular ChT, TCA, LA, SA, and CVI were all significantly lower in the more myopic (longer AL) eyes than in the less myopic (shorter AL) fellow eyes. In eyes with longer ALs, both the CcFD and FAZ areas were significantly greater, whereas the superficial and deep retinal VDs were significantly less. After adjusting for corneal power and intraocular pressure, interocular differences in LA (β = -0.774), SA (β = -0.991), and CcFD (β = 0.040) were significantly associated with interocular asymmetry in AL (all P < 0.05).

Conclusions: In pediatric anisomyopes, eyes with longer ALs tended to have lower choroidal vascularity and choriocapillaris perfusion than the contralateral eyes with shorter ALs. Longitudinal investigations would be useful follow-ups to test for a causal role of choroidal circulation in human myopia.
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http://dx.doi.org/10.1167/iovs.62.9.40DOI Listing
July 2021

Robot-Assisted Electrode Array Insertion Becomes Available in Pediatric Cochlear Implant Recipients: First Report and an Intra-Individual Study.

Front Surg 2021 7;8:695728. Epub 2021 Jul 7.

Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

As an advanced surgical technique to reduce trauma to the inner ear, robot-assisted electrode array (EA) insertion has been applied in adult cochlear implantation (CI) and was approved as a safe surgical procedure that could result in better outcomes. As the mastoid and temporal bones are generally smaller in children, which would increase the difficulty for robot-assisted manipulation, the clinical application of these systems for CI in children has not been reported. Given that the pediatric candidate is the main population, we aim to investigate the safety and reliability of robot-assisted techniques in pediatric cochlear implantation. Retrospective cohort study at a referral center in Shanghai including all patients of simultaneous bilateral CI with robotic assistance on one side (RobOtol® system, Collin ORL, Bagneux, France), and manual insertion on the other (same brand of EA and CI in both side), from December 2019 to June 2020. The surgical outcomes, radiological measurements (EA positioning, EA insertion depth, mastoidectomy size), and audiological outcomes (Behavior pure-tone audiometry) were evaluated. Five infants (17.8 ± 13.5 months, ranging from 10 to 42 months) and an adult (39 years old) were enrolled in this study. Both perimodiolar and lateral wall EAs were included. The robot-assisted EA insertion was successfully performed in all cases, although the surgical zone in infants was about half the size in adults, and no difference was observed in mastoidectomy size between robot-assisted and manual insertion sides ( = 0.219). The insertion depths of EA with two techniques were similar ( = 0.583). The robot-assisted technique showed no scalar deviation, but scalar deviation occurred for one manually inserted pre-curved EA (16%). Early auditory performance was similar to both techniques. Robot-assisted technique for EA insertion is approved to be used safely and reliably in children, which is possible and potential for better scalar positioning and might improve long-term auditory outcome. Standard mastoidectomy size was enough for robot-assisted technique. This first study marks the arrival of the era of robotic CI for all ages.
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http://dx.doi.org/10.3389/fsurg.2021.695728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294934PMC
July 2021

Syphilis Testing as a Proxy Marker for a Subgroup of Men Who Have Sex With Men With a Central Role in HIV-1 Transmission in Guangzhou, China.

Front Med (Lausanne) 2021 7;8:662689. Epub 2021 Jul 7.

Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, China.

The objectives of this study were to distinguish the role of men who have sex with men (MSM) with or without syphilis testing in HIV-1 transmission and to provide molecular evidence of syphilis testing as a proxy marker for identifying the subgroup of MSM. HIV-1 transmission clusters were constructed by HIV-TRACE and Cluster Picker using HIV-1 pol sequences from 729 newly diagnosed HIV-infected MSM from 2008 to 2012 in Guangzhou, China. The role of MSM in HIV-1 transmission networks was determined by a node influence measurement and centrality analysis. The association between syphilis testing and factors related to HIV-1 transmission and antiretroviral treatment (ART) were analyzed by the Cox regression model. Among HIV-infected MSM, 56.7% did not test for syphilis at the time of HIV-1 diagnosis. MSM without syphilis testing was a specific subgroup of MSM with a larger closeness centrality and clustering coefficient than the recipients of syphilis testing ( < 0.001), indicating their central position in the HIV-1 transmission networks. The median degree and radiality within HIV-1 transmission networks as well as the median K-shell scores were also greater for MSM without syphilis testing ( < 0.001), suggesting their relatively greater contribution in transmitting HIV-1 than the receipts of syphilis testing. MSM with syphilis testing usually did not disclose their occupation or were more likely to be unemployed or to take non-skilled jobs, to have a history of sexually transmitted infections (STIs), and to be AIDS patients when diagnosed with HIV-1 infection ( < 0.05). Multivariable Cox regression analysis indicated that syphilis testing did not promote the engagement of ART ( = 0.233) or affect the speed of CD4 T cell count recovery after treatment ( = 0.256). Our study identifies syphilis testing as a proxy marker of a specific subgroup of HIV-infected MSM who refuse syphilis testing during HIV-1 diagnosis with an important role in HIV-1 transmission. Specific prevention and intervention targeting MSM without syphilis testing during HIV-1 care are urgently needed.
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http://dx.doi.org/10.3389/fmed.2021.662689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293274PMC
July 2021

Effects of Tai Chi Softball Exercises on Physical Fitness Level and Cardiovascular Health-Related Factors among Older Females.

J Healthc Eng 2021 2;2021:7671596. Epub 2021 Jul 2.

Capital University of Physical Education and Sports, Beijing 100191, China.

Tai Chi softball was voted as one of the most popular health-promoting exercises and can also develop manipulative skill and hand-eye coordination. The purpose of this study was to explore changes in the physical fitness level and cardiovascular health-related factors after the 16-week Tai Chi softball (TCSB) training program among older females. One hundred healthy older females were assessed at the baseline before TCSB training, and then, they conducted intervention experiments. Through the 16-week TCSB intervention training, a control group and a physical exercise group showed significant differences. The physical exercise group significantly reduced body mass index, systolic blood pressure, and diastolic blood pressure from all the results studied. It also reduces total cholesterol, triglycerides, low-density lipoprotein cholesterol ( < 0.05), and waist-to-hip ratio ( < 0.01), whereas handgrip, sit and reach, single leg stance, vital capacity ( < 0.05), and high-density lipoprotein cholesterol ( < 0.01) were significantly increased. TCSB training may improve physical fitness ability and decrease the risk of cardiovascular disease among older females.
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http://dx.doi.org/10.1155/2021/7671596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272665PMC
July 2021

The Effect of Various Si/Al, Na/Al Molar Ratios and Free Water on Micromorphology and Macro-Strength of Metakaolin-Based Geopolymer.

Materials (Basel) 2021 Jul 9;14(14). Epub 2021 Jul 9.

School of Civil Engineering, Northeast Forestry University, Harbin 150040, China.

The current work aimed to explore the effect of Na/Al ratios of 0.43, 0.53, 0.63, 0.73, 0.83, and 0.93, using NaOH to alter the molar ratio, on the mechanical properties of a geopolymer material, with fixing of the Si/Al molar ratio. While fixing the Na/Al molar ratio, alteration of the Si/Al ratios to 1.7, 1.75, 1.8, 1.85, 1.9, 1.95 was used, with silica fume and sodium silicate as a silica corrector. The influence on the micromorphology and macro-strength of samples was characterized through SEM, EDS, and compressive strength characterization methods. The results show that Si/Al and Na/Al molar ratios play a significant role in the microstructure and mechanical behavior of MK-based geopolymers, and revealed that the optimal molar Si/Al and Na/Al ratios for attaining maximum mechanical strength in geopolymers are 1.9 and 0.73, respectively. Under various Si/Al ratios, the macro-strength of the geopolymer mainly relies on the formation of NASH gel, rather than zeolites or silicate derivatives. The appropriate Na/Al molar ratio can contribute to the geopolymerization, but a ultra-high Na/Al molar ratio caused a high alkali state that destroyed the microstructure of the geopolymers. Regardless of the amount of water contained in the initial geopolymer raw material, the water content of Si/Al = 1.65 and Si/Al = 1.75 after curing for 10 days was almost the same, and the bound water content of the final geopolymer was maintained at about 15%. Structural water exists in geological polymer gels in the form of a chemical structure. It has effects on the structural performance strength, while free water affects the volume stability of the geological polymer. Overall, the current work provides a perspective on the elemental composition analysis, combined with the molecular structure and micromorphology, to explore the mechanical performance of geopolymers.
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http://dx.doi.org/10.3390/ma14143845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305878PMC
July 2021

Leisure activity and cognitive function among Chinese old adults: The multiple mediation effect of anxiety and loneliness.

J Affect Disord 2021 Jul 17;294:137-142. Epub 2021 Jul 17.

Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, No.1163 Xinmin Street, Changchun 130021, Jilin province, China. Electronic address:

Background: Most countries in the world, including China, are experiencing serious aging problems. The decline of cognitive function seriously affects the quality of life of the elderly in their later years and brings an inevitable heavy burden to the family and society. Therefore, in order to achieve successful aging, the purpose of this study is to test the serial multiple mediation effect of anxiety and loneliness between leisure activity and cognitive function in Chinese elderly population.

Methods: Using 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS) dataset, we finally selected 6,525 Chinese elderly people over 65 years old after screening. Firstly, we described the basic social demographic information of the sample population. Secondly, Spearman correlation analysis was used to determine whether there is a correlation between leisure activity, anxiety, loneliness and cognitive function in Chinese elderly. Finally, the serial multiple mediation analysis was completed using the SPSS macro PROCESS program.

Results: Leisure activity, anxiety, loneliness and cognitive function were significantly correlated (p<0.01). Leisure activity can not only have a direct positive impact on the cognitive function of the elderly (effect=0.2231; SE=0.0122; 95%CI: LL=0.1992, UL=0.2470), but also have an indirect impact on the cognitive function through three paths: the independent mediating role of anxiety (effect=0.0028; SE=0.0015; 95%CI: LL=0.0001, UL=0.0059), the independent mediating role of loneliness (effect=0.0032; SE=0.0016; 95%CI: LL=0.0002, UL=0.0065), and the chain mediating role of anxiety and loneliness (effect=0.0008; SE=0.0004; 95%CI: LL=0.0001, UL=0.0017).

Limitations: All items were self-reported and some results may have biased. In the future, it may be more instructive to explore the impact of specific leisure activity on the cognitive function of the elderly.

Conclusions: The study suggests that leisure activity can improve cognitive function through decreasing anxiety and loneliness among the Chinese elderly. Diversified interventions aimed at increasing leisure activity participation in older adults would be beneficial for their mental health and cognitive function.
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http://dx.doi.org/10.1016/j.jad.2021.07.051DOI Listing
July 2021

Identification and characterization of amphibian SLC26A5 using RNA-Seq.

BMC Genomics 2021 Jul 22;22(1):564. Epub 2021 Jul 22.

Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Prestin (SLC26A5) is responsible for acute sensitivity and frequency selectivity in the vertebrate auditory system. Limited knowledge of prestin is from experiments using site-directed mutagenesis or domain-swapping techniques after the amino acid residues were identified by comparing the sequence of prestin to those of its paralogs and orthologs. Frog prestin is the only representative in amphibian lineage and the studies of it were quite rare with only one species identified.

Results: Here we report a new coding sequence of SLC26A5 for a frog species, Rana catesbeiana (the American bullfrog). In our study, the SLC26A5 gene of Rana has been mapped, sequenced and cloned successively using RNA-Seq. We measured the nonlinear capacitance (NLC) of prestin both in the hair cells of Rana's inner ear and HEK293T cells transfected with this new coding gene. HEK293T cells expressing Rana prestin showed electrophysiological features similar to that of hair cells from its inner ear. Comparative studies of zebrafish, chick, Rana and an ancient frog species showed that chick and zebrafish prestin lacked NLC. Ancient frog's prestin was functionally different from Rana.

Conclusions: We mapped and sequenced the SLC26A5 of the Rana catesbeiana from its inner ear cDNA using RNA-Seq. The Rana SLC26A5 cDNA was 2292 bp long, encoding a polypeptide of 763 amino acid residues, with 40% identity to mammals. This new coding gene could encode a functionally active protein conferring NLC to both frog HCs and the mammalian cell line. While comparing to its orthologs, the amphibian prestin has been evolutionarily changing its function and becomes more advanced than avian and teleost prestin.
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http://dx.doi.org/10.1186/s12864-021-07798-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296623PMC
July 2021

Microbial production, molecular modification, and practical application of l-Asparaginase: A review.

Int J Biol Macromol 2021 Jul 19;186:975-983. Epub 2021 Jul 19.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.

L-Asparaginase (L-ASNase, EC 3.5.1.1), an antitumor drug for acute lymphoblastic leukemia (ALL) therapy, is widely used in the clinical field. Similarly, L-ASNase is also a powerful and significant biological tool in the food industry to inhibit acrylamide (AA) formation. This review comprehensively summarizes the latest achievements and improvements in the production, modification, and application of microbial L-ASNase. To date, the expression levels and optimization of expression hosts such as Escherichia coli, Bacillus subtilis, and Pichia pastoris, have made significant progress. In addition, examples of successful modification of L-ASNase such as decreasing glutaminase activity, increasing the in vivo stability, and enhancing thermostability have been presented. Impressively, the application of L-ASNase as a food addition aid, as well as its commercialization in the pharmaceutical field, and cutting-edge biosensor application developments have been summarized. The presented results and proposed ideas could be a good guide for other L-ASNase researchers in both scientific and practical fields.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.107DOI Listing
July 2021

Molecular evidence suggesting the persistence of residual SARS-CoV-2 and immune responses in the placentas of pregnant patients recovered from COVID-19.

Cell Prolif 2021 Jul 22:e13091. Epub 2021 Jul 22.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Objectives: Recent studies have shown the presence of SARS-CoV-2 in the tissues of clinically recovered patients and persistent immune symptoms in discharged patients for up to several months. Pregnant patients were shown to be a high-risk group for COVID-19. Based on these findings, we assessed SARS-CoV-2 nucleic acid and protein retention in the placentas of pregnant women who had fully recovered from COVID-19 and cytokine fluctuations in maternal and foetal tissues.

Materials And Methods: Remnant SARS-CoV-2 in the term placenta was detected using nucleic acid amplification and immunohistochemical staining of the SARS-CoV-2 protein. The infiltration of CD14+ macrophages into the placental villi was detected by immunostaining. The cytokines in the placenta, maternal plasma, neonatal umbilical cord, cord blood and amniotic fluid specimens at delivery were profiled using the Luminex assay.

Results: Residual SARS-CoV-2 nucleic acid and protein were detected in the term placentas of recovered pregnant women. The infiltration of CD14+ macrophages into the placental villi of the recovered pregnant women was higher than that in the controls. Furthermore, the cytokine levels in the placenta, maternal plasma, neonatal umbilical cord, cord blood and amniotic fluid specimens fluctuated significantly.

Conclusions: Our study showed that SARS-CoV-2 nucleic acid (in one patient) and protein (in five patients) were present in the placentas of clinically recovered pregnant patients for more than 3 months after diagnosis. The immune responses induced by the virus may lead to prolonged and persistent symptoms in the maternal plasma, placenta, umbilical cord, cord blood and amniotic fluid.
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http://dx.doi.org/10.1111/cpr.13091DOI Listing
July 2021

Construction of an immune-related lncRNA pairs model to predict prognosis and immune landscape of lung adenocarcinoma patients.

Bioengineered 2021 Dec;12(1):4123-4135

Division of Thoracic Surgery, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, China.

The model of immune-related lncRNA pairs (IRLPs) seems to be an available predictor in lung adenocarcinoma (LUAD) patients. The aim of our study was to construct a model with IRLPs to predict the survival status and immune landscape of LUAD patients. Based on TCGA-LUAD dataset, a risk assessment model with IRLPs was established. Then, ROC curves were used to assess the predictive accuracy and effectiveness of our model. Next, we identified the difference of survival, immune cell infiltration, immune checkpoint inhibitor-related (ICI-related) biomarkers, and chemotherapeutics between high-risk group and low-risk group. Finally, A nomogram was built for predicting the survival rates of LUAD patients. 464 LUAD samples were randomly and equally divided into a training set and a test set. Six IRLPs were screened out to construct a risk model. K-M analysis and risk-plot suggested the prognosis of high-risk group was worse than low-risk group (p < 0.001). Multivariate analysis shows risk score was independent risk factor of LUAD (p < 0.001). In addition, the expression of immune cell infiltration, ICI-related biomarkers, chemotherapeutics all demonstrate significant difference in two groups. A nomogram was built that could predict the 1-, 3-, and 5-year survival rates of LUAD patients. Our immune-related lncRNA pairs risk model is expected to be a reliable model for predicting the prognosis and immune landscape of LUAD patients.
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http://dx.doi.org/10.1080/21655979.2021.1953215DOI Listing
December 2021

Programmed PPAR-α downregulation induces inflammaging by suppressing fatty acid catabolism in monocytes.

iScience 2021 Jul 24;24(7):102766. Epub 2021 Jun 24.

Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China.

Inflammaging is associated with an increased risk of chronic disease. Monocytes are the principal immune cells for the production of inflammatory cytokines and contribute to inflammaging in the elderly. However, the underlying mechanisms remain largely unknown. Here, we found that monocytes from aged individuals contained high levels of lipid droplets (LDs), and this increase was correlated with impaired fatty acid oxidation. Downregulated peroxisome proliferator-activated receptor (PPAR)-α may be responsible for the pro-inflammatory phenotype of monocytes in aged individuals, as it was positively correlated with LD accumulation and increasing TNF-α concentration. Interestingly, interventions that result in PPAR-α upregulation, such as fenofibrate treatment, TNF-α neutralization, or calorie restriction, reversed the effect of aging on monocytes. Thus the downregulation of PPAR-α and LD levels in monocytes represents a novel biomarker for inflammaging. Furthermore, PPAR-α activation in the elderly may also alleviate long-term inflammaging, preventing the development of life-limiting chronic diseases.
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http://dx.doi.org/10.1016/j.isci.2021.102766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273418PMC
July 2021

FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat.

mBio 2021 Jul 20:e0079521. Epub 2021 Jul 20.

State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology, Ministry of Education, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, China.

Human immunodeficiency virus type 1 (HIV-1) cannot be completely eliminated because of existence of the latent HIV-1 reservoir. However, the facts of HIV-1 latency, including its establishment and maintenance, are incomplete. FKBP3, encoded by the gene, belongs to the immunophilin family of proteins and is involved in immunoregulation and such cellular processes as protein folding. In a previous study, we found that FKBP3 may be related to HIV-1 latency using CRISPR screening. In this study, we knocked out the gene in multiple latently infected cell lines to promote latent HIV-1 activation. We found that FKBP3 could indirectly bind to the HIV-1 long terminal repeat through interaction with YY1, thereby recruiting histone deacetylase 1/2 to it. This promotes histone deacetylation and induces HIV-1 latency. Finally, in a primary latent cell model, we confirmed the effect of knockout on the latent activation of HIV-1. Our results suggest a new mechanism for the epigenetic regulation of HIV-1 latency and a new potential target for activating latent HIV-1. The primary reason why AIDS cannot be completely cured is the existence of a latent HIV-1 reservoir. Currently, the facts of HIV-1 latency, including its establishment and maintenance, are incomplete. Using a CRISPR library in our earlier screening of genes related to HIV-1 latency, we identified as a candidate gene related to HIV-1 latency. Therefore, in this mechanistic study, we first confirmed the HIV-1 latency-promoting effect of FKBP3 and determined that FKBP3 promotes histone deacetylation by recruiting histone deacetylase 1/2 to the HIV-1 long terminal repeat. We also confirmed, for the first time, that FKBP3 can act as a transcription factor (TF) recruitment scaffold and participate in epigenetic regulation of HIV-1 latency. These findings suggest a new mechanism for the epigenetic regulation of HIV-1 latency and a new potential target for activating latent HIV-1.
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http://dx.doi.org/10.1128/mBio.00795-21DOI Listing
July 2021

Cleaving arene rings for acyclic alkenylnitrile synthesis.

Nature 2021 Jul 19. Epub 2021 Jul 19.

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.

Synthetic chemistry is built around the formation of carbon-carbon bonds. Conversely, selective methods for C-C bond cleavage is a largely unmet challenge, the solution of which will provide promising applications in synthesis, coal liquefaction, petroleum cracking, polymer degradation and biomass conversion. For example, aromatic rings are ubiquitous skeletal features in inert chemical feed stocks, but are inert to many reaction conditions owing to their aromaticity and low polarity. Over the past century, only a few methods under harsh conditions have achieved direct arene ring modifications involving the cleavage of inert aromatic C-C bonds, and arene ring-cleavage reactions using stoichiometric transition metal complexes or enzymes in bacteria are still limited. We now report a copper-catalysed selective arene ring-opening reaction strategy. Our aerobic oxidative copper catalysis converts anilines, arylboronic acids, aryl azides, aryl halides, aryl triflates, aryl trimethylsiloxanes, aryl hydroxamic acids and aryl diazonium salts into alkenyl nitriles through selective C-C bond cleavage of arene rings. This chemistry was applied to the modification of polycyclic aromatics and the preparation of industrially important hexamethylenediamine and adipic acid derivatives. Several examples of late-stage modification of complex molecules and fused ring compounds further support the potential broad utility of this methodology.
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http://dx.doi.org/10.1038/s41586-021-03801-yDOI Listing
July 2021

Facile one-step synthesis of 3D honeycomb-like porous chitosan bead inlaid with MnFe bimetallic oxide nanoparticles for enhanced degradation of dye pollutant.

Int J Biol Macromol 2021 Jul 16;186:829-838. Epub 2021 Jul 16.

School of Basic Medical Sciences, Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education of China, Xi'an 710061, China. Electronic address:

Developing a sustainable, efficient and recyclable heterogeneous Fenton-like catalyst is important to wastewater treatment. Herein, well-dispersed MnO and FeO nanoparticles inlaid in chitosan beads (MnO-FeO/CH) was firstly fabricated and employed in the degradation of methylene blue (MB). The bead was prepared via a facile one-step method by dropwise addition of chitosan-metal salt solution into alkaline solution. Comparing with monometallic chitosan beads (MnO/CH, FeO/CH) and naked MnO-FeO, MnO-FeO/CH displayed significantly higher activity for MB degradation with the assistance of hydrogen peroxide (HO), finally removing 96.8% MB under the optimal conditions (50 mg L MB, 4.0 g L catalyst, 30 g L HO, pH = 7, 60 min). Based on a series of characterizations, the large surface area (60.1 m g), well-developed porosity (0.3 cm g), and intensified electron transport of MnO-FeO/CH consequently enhanced the catalytic performance via a synergistic effect. Because the specific porous structure of MnO-FeO/CH facilitated the adsorption/diffusion of reactants and exposure of active sites. Meanwhile, the electron transfer from Mn to Fe accelerated the Fe/Fe cycle, which favored the production of dominant reactive species hydroxyl radical for MB degradation. Besides, the magnetic beads could be easily collected from the solution and reused for five times with a negligible leaching.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.090DOI Listing
July 2021

A quantitative image-based protocol for morphological characterization of cellular solids in feather shafts.

STAR Protoc 2021 Sep 6;2(3):100661. Epub 2021 Jul 6.

Integrative Stem Cell Center, China Medical University Hospital, Taichung 40447, Taiwan.

During morphogenesis, cellular sheets undergo dynamic folding to build functional forms. Here, we develop an image-based quantitative morphology field (QMorF) protocol that quantifies the morphological features of cellular structures and associated distributions. Using feather shafts with different rigidities as examples, QMorF performs coarse-graining statistical measurements of the fitted cellular objects over a micro-image stack, revealing underlying mechanical coupling and developmental clues. These images give intuitive representations of mechanical forces and should be useful for analyzing tissue images showing clear cellular features. For complete details on the use and execution of this protocol, please refer to Chang et al. (2019).
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http://dx.doi.org/10.1016/j.xpro.2021.100661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271172PMC
September 2021

Leadership Styles and Organizational Citizenship Behavior for the Environment: The Mediating Role of Self-Efficacy and Psychological Ownership.

Front Psychol 2021 2;12:683101. Epub 2021 Jul 2.

Hailey College of Commerce, University of the Punjab, Lahore, Pakistan.

Change and environmental patterns are having an immense effect upon the world. Businesses, communities, and even individuals are struggling to perform their role within environmental protection. This paper investigates the role of leadership styles on organizational citizenship behavior for the environment (OCBE) directly and through the mediation of self-efficacy and psychological ownership. The survey technique was used to collect the data from Chinese banking, insurance, medicine, and teaching service sector employees for the current study. The reliability and validity of the scale items were tested. This study used AMOS-SEM for data analysis and testing the developed hypotheses. The empirical results confirmed that responsible, inclusive, authentic, and supportive leadership styles positively impact employees' OCBE. The results further confirm that self-efficacy and psychological ownership act as mediators between leadership and OCBE. The current study widens our understanding of leadership styles and their impact on OCBE, along with limitations associated with the study and future guidelines for investigators.
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http://dx.doi.org/10.3389/fpsyg.2021.683101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283275PMC
July 2021

Cytotoxicity and genotoxicity of low-dose vanadium dioxide nanoparticles to lung cells following long-term exposure.

Toxicology 2021 Jul 15;459:152859. Epub 2021 Jul 15.

Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai, 200444, China. Electronic address:

Vanadium dioxide nanoparticles (VO NPs) have been massively produced and widely applied due to their excellent metal-insulator transition property, making it extremely urgent to evaluate their safety, especially for low-dose long-term respiratory occupational exposure. Here, we report a comprehensive cytotoxicity and genotoxicity study on VO NPs to lung cell lines A549 and BEAS-2B following a long-term exposure. A commercial VO NP, S-VO, was used to treat BEAS-2B (0.15-0.6 μg/mL) and A549 (0.3-1.2 μg/mL) cells for four exposure cycles, and each exposure cycle lasted for 4 consecutive days; then various bioassays were performed after each cycle. Significant proliferation inhibition was observed in both cell lines after long-term exposure of S-VO at low doses that did not cause apparent acute cytotoxicity; however, the genotoxicity of S-VO, characterized by DNA damage and micronuclei, was only observed in A549 cells. These adverse effects of S-VO were exposure time-, dose- and cell-dependent, and closely related to the solubility of S-VO. The oxidative stress in cells, i.e., enhanced reactive oxygen species (ROS) generation and suppressed reduced glutathione, was the main toxicity mechanism of S-VO. The ROS-associated mitochondrial damage and DNA damage led to the genotoxicity, and cell proliferation retard, resulting in the cellular viability loss. Our results highlight the importance and urgent necessity of the investigation on the long-term toxicity of VO NPs.
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http://dx.doi.org/10.1016/j.tox.2021.152859DOI Listing
July 2021

Field tests of crop growth using hydrothermal and spray-dried cephalosporin mycelia dregs as amendments: Utilization of nutrient and soil antibiotic resistome.

Environ Res 2021 Jul 15;202:111638. Epub 2021 Jul 15.

School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, Shanxi, 710049, China. Electronic address:

The disposal and reuse of cephalosporin mycelia dregs (CMDs) pose a great challenge to the biopharma industry, but it acts as the new source of antibiotic resistome, although agriculture intensification remains uncertain. Herein, two common cash crops (maize and soybean) were planted in the actual field, and the effects of the application of treated CMDs, chicken manure and chemical fertilizer served as control groups were both investigated according to comparison experiment. Amplicon-targeted 16S rRNA and high-throughput sequencing was analyzed for rhizosphere antibiotic resistome. Results showed that hydrothermal and spray-dried (HT + SD) CMDs could promote nutrients uptake and stabilize soil fertility indicator, and finally improved the crop yield (maximum, 119.68%). The numbers and relative abundances of total ARGs in soils were not significantly different from that of conventional fertilizer (p > 0.05), but crop type marked the differences in distribution. The overall economic benefits are predicted to be around $373-745 million annually, considering its application to the whole country. HT + SD-treated CMDs can be therefore used as a high-quality and safe alternative fertilizer for agriculture use. These findings are expected to offer a fresh perspective on the application of antibiotic fermentation residue (AFR) in the future.
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http://dx.doi.org/10.1016/j.envres.2021.111638DOI Listing
July 2021

Modular synthesis of 3-substituted isocoumarins silver-catalyzed aerobic oxidation/ heterocyclization of -alkynylbenzaldehydes.

Org Biomol Chem 2021 Jul 16. Epub 2021 Jul 16.

School of Chemistry and Materials Science, Jiangsu Key Laboratory of Green Synthesis for Functional Materials, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.

A method involving silver-catalyzed aerobic oxidation/6-endo heterocyclization of ortho-alkynylbenzaldehydes to yield 3-substituted isocoumarins is described. The developed protocol allows convenient access to a range of synthetically useful 3-substituted isocoumarins and related fused heterocyclolactones in good to high yields, using silver tetrafluoroborate as the catalyst, and atmospheric oxygen as the terminal oxidant and the source of endocyclic oxygen. Mechanistic studies suggest the involvement of a free-radical pathway.
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http://dx.doi.org/10.1039/d1ob01065dDOI Listing
July 2021

Mechanisms for the dissolved biochar promoted iron dissolution and consequential chromium release.

Sci Total Environ 2021 Jul 9;796:148923. Epub 2021 Jul 9.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China. Electronic address:

Biochar is widely applied in soil for agricultural and environmental purposes. Soluble fraction of biochar may be released from bulk biochar as dissolved biochar (DBC) after irrigation or rainfall. DBC had been reported to possess high chemical activity in aqueous system, while less attention was paid to the impact of DBC on the soil environmental processes. In this work, the impact of DBC on ferric (hydro) oxides was systematically examined. Our study showed that DBC prepared from rice straw could significantly promote the dissolution of ferric oxides with unstable and metastable crystalline structures, e.g., ferrihydrite under relatively acidic condition. Organic ligand-promoted dissolution was the main mechanism for iron release from ferrihydrite, and the low-molecular-weight DBC component (less than 1000 Da) was the major contributor for this process. Furthermore, the organic carbon content normalized ligand-promoted dissolution capacity for DBC was much higher than common dissolved organic matters. More importantly, DBC could promote the release of Cr from dichromate-adsorbed ferric mineral. Our results suggest that in soils with relatively low pH and high contents of ferric hydroxides, e.g., red soil in southern China, DBC derived from applied biochar could enhance the mobility and bioavailability of iron and other heavy metals. The dissolved metals would play active roles in soil redox cycle and biotic processes. Therefore, it's necessary to evaluate the long-term impact of biochar application on acidic field soils with high iron content.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148923DOI Listing
July 2021

Spatial distribution and morphological transformation of chromium with coexisting substances in tannery landfill.

Chemosphere 2021 Jul 9;285:131503. Epub 2021 Jul 9.

College of Architecture and Environment, Sichuan University, Chengdu, 610065, China. Electronic address:

The prosperity and development of tannery industry have brought about rapid economic growth. However, the tannery landfill without anti-seepage measures in the early stage has generated masses of environmental hazards owing to the lack of awareness in environmental protection. Therefore, it is imperative to pay much attention to the understanding of environmental hazards from tannery waste. In this study, solid samples and groundwater samples were collected from a tannery landfill to study the effect of the characteristic pollutants produced by tanning on chromium distribution with other coexisting substances. The results showed that significant correlations were demonstrated between multiple coexisting substances (total organic carbon, total petroleum hydrocarbons, total nitrogen, Cr, F, Ca, Cu and Pb), indicating the possible same source or they coming from the same tannery production stage. The weights of positive effects and negative effects of coexisting substances on total Cr distribution in the profile decreased in the order: total nitrogen > Cu > Ca > Pb > total organic carbon > F > SO> Cd, and Ni > Cl > Hg, respectively. Moreover, the simulation of Visual MINTEQ showed that the cations were mainly bound to Cr as CrO, while the anions were bound to Cr. This study provided a new perspective on the selection of remediation strategies for Cr-contaminated sites to avoid secondary environmental pollution caused by the release of coexisting heavy metals.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131503DOI Listing
July 2021

Directly observing the skew angle of a Poynting vector in an OAM carrying beam via angular diffraction.

Opt Lett 2021 Jul;46(14):3484-3487

A new, to the best of our knowledge, method for directly measuring the skew angle of a Poynting vector of optical vortices is reported in this Letter. We design an incomplete optical vortex phase to mimic the occlusion of actual objects on the light path. By capturing the intensity cross section of the incomplete vortex field, the energy flow can be observed directly; thus, the skew angle of the Poynting vector can be directly measured. In this Letter, we measure the skew angle of the Poynting vector with an error less than 3%. Further, the work in this Letter may provide a new way to sense the translational distance and measure the topological charge of the optical vortex.
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http://dx.doi.org/10.1364/OL.432771DOI Listing
July 2021

Decreased expression of the clock gene Bmal1 is involved in the pathogenesis of temporal lobe epilepsy.

Mol Brain 2021 Jul 14;14(1):113. Epub 2021 Jul 14.

Department of Neurosurgery, Clinical Research Center for Refractory Epilepsy of Shannxi Province, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, Shaanxi, China.

Clock genes not only regulate the circadian rhythm of physiological activities but also participate in the pathogenesis of many diseases. Previous studies have documented the abnormal expression of clock genes in epilepsy. However, the molecular mechanism of brain and muscle Arnt-like protein 1 (Bmal1), one of the core clock genes, in the epileptogenesis and seizures of temporal lobe epilepsy (TLE) remain unclear. We first investigated the levels of Bmal1 and other clock proteins in the hippocampus of subjects with epilepsy to define the function of Bmal1. The levels of Bmal1 were decreased during the latent and chronic phases in the experimental group compared with those in the control group. Knockout of Bmal1 in hippocampal dentate gyrus (DG) neurons of Bmal1 mice by Synapsin 1 (Syn1) promoter AAV (adeno-associated virus) lowered the threshold of seizures induced by pilocarpine administration. High-throughput sequencing analysis showed that PCDH19 (protocadherin 19), a gene associated with epilepsy, was regulated by Bmal1. PCDH19 expression was also decreased in the hippocampus of epileptic mice. Furthermore, the higher levels of Bmal1 and PCDH19 were detected in patients with no hippocampal sclerosis (no HS) than in patients with HS International League Against Epilepsy (ILAE) type I and III. Altogether, these data suggest that decreased expression of clock gene Bmal1 may participate in epileptogenesis and seizures via PCDH19 in TLE.
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http://dx.doi.org/10.1186/s13041-021-00824-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281660PMC
July 2021

Insulin Fibril Formation Caused by Mechanical Shock and Cavitation.

J Phys Chem B 2021 Jul 14;125(29):8021-8027. Epub 2021 Jul 14.

Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, Colorado 80303, United States.

Cavitation can occur when liquids are exposed to pressure waves of sufficient amplitude, producing rapidly expanding and collapsing gas bubbles that generate localized regions of high energy dissipation. When vials containing insulin were subjected to mechanical shock or when ultrasound was applied to the vials, the resulting cavitation events induced formation of insulin amyloid fibril nuclei that were detected by transmission electron microscopy and quantified by fluorescence spectroscopy following staining with the amyloid-sensitive dye thioflavin-T. Dropping insulin solutions in glass vials produced only minute amounts of insulin fibril nuclei, which could be detected by allowing the nuclei to grow. Cavitation-induced formation of amyloid aggregates may be relevant for iatrogenic insulin deposition disease, where insulin fibrils formed in vitro prior to administration to patients could serve as nuclei for growing fibril deposits in vivo.
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http://dx.doi.org/10.1021/acs.jpcb.1c01997DOI Listing
July 2021

Decoding imagined speech from EEG signals using hybrid-scale spatial-temporal dilated convolution network.

J Neural Eng 2021 Jul 13. Epub 2021 Jul 13.

Xidian University, No. 2 South Taibai Road, Xi'an, Shaanxi, Xian, Shaanxi, 710071, CHINA.

Objective: Directly decoding imagined speech from electroencephalogram (EEG) signals has attracted much interest in brain-computer interface applications, because it provides a natural and intuitive communication method for locked-in patients. Several methods have been applied to imagined speech decoding, but how to construct spatial-temporal dependencies and capture long-range contextual cues in EEG signals to better decode imagined speech should be considered.

Approach: In this study, we propose a novel model called hybrid-scale spatial-temporal dilated convolution network (HS-STDCN) for EEG-based imagined speech recognition. HS-STDCN integrates feature learning from temporal and spatial information into a unified end-to-end model. To characterize the temporal dependencies of the EEG sequences, we adopted a hybrid-scale temporal convolution layer to capture temporal information at multiple levels. A depthwise spatial convolution layer was then designed to construct intrinsic spatial relationships of EEG electrodes, which can produce a spatial-temporal representation of the input EEG data. Based on the spatial-temporal representation, dilated convolution layers were further employed to learn long-range discriminative features for the final classification.

Main Results: To evaluate the proposed method, we compared the HS-STDCN with other existing methods on our collected dataset. The HS-STDCN achieved an averaged classification accuracy of 54.31\% for decoding eight imagined words, which is significantly better than other methods at a significance level of 0.05.

Significance: The proposed HS-STDCN model provided an effective approach to make use of both the temporal and spatial dependencies of the input EEG signals for imagined speech recognition. We also visualized the word semantic differences to analyze the impact of word semantics on imagined speech recognition, investigated the important regions in the decoding process, and explored the use of fewer electrodes to achieve comparable performance.
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http://dx.doi.org/10.1088/1741-2552/ac13c0DOI Listing
July 2021

Integrative analysis the characterization of peroxiredoxins in pan-cancer.

Cancer Cell Int 2021 Jul 10;21(1):366. Epub 2021 Jul 10.

Department of Urology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, China.

Background: Peroxiredoxins (PRDXs) are an antioxidant enzymes protein family involved in several biological functions such as differentiation, cell growth. In addition, previous studies report that PRDXs play critical roles in the occurrence and development of carcinomas. However, few studies have conducted systematic analysis of PRDXs in cancers. Therefore, the present study sought to explore the molecular characteristics and potential clinical significance of PRDX family members in pan cancer and further validate the function of PRDX6 in bladder urothelial carcinoma (BLCA).

Methods: A comprehensive analysis of PRDXs in 33 types of cancer was performed based on the TCGA database. This involved an analysis of mRNA expression profiles, genetic alterations, methylation, prognostic values, potential biological pathways and target drugs. Moreover, both the gain and loss of function strategies were used to assess the importance and mechanism of PRDX6 in the cell cycle of BLCA.

Result: Analysis showed abnormal expression of PRDX1-6 in several types of cancer compared to normal tissues. Univariate Cox proportional hazard regression analysis showed that expression levels of PRDX1, PRDX4 and PRDX6 were mostly associated with poor survival of OS, DSS and PFI, and PRDX2 and PRDX3 with favorable survival. In addition, the expression of PRDX genes were positively correlated with CNV and negatively with methylation. Moreover, analysis based on PharmacoDB dataset showed that the augmented levels of PRDX1, PRDX3 and PRDX6 were significantly correlated with EGFR/VEGFR inhibitor drugs. Furthermore, knocking down of PRDX6 inhibited growth of cancer cells through the JAK2-STAT3 in bladder cell lines.

Conclusions: PRDXs are potential biomarkers and therapeutic targets for several carcinomas, especially for BLCA. In addition, PRDX6 could regulate proliferation of cancer cell via JAK2-STAT3 pathway and involve into the process of cell cycle in BLCA.
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http://dx.doi.org/10.1186/s12935-021-02064-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272277PMC
July 2021

Retraction Note to: Methylene blue photochemical treatment as a reliable SARS-CoV-2 plasma virus inactivation method for blood safety and convalescent plasma therapy for COVID-19.

BMC Infect Dis 2021 Jul 9;21(1):672. Epub 2021 Jul 9.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Disease, National Medical Center for Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, China.

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http://dx.doi.org/10.1186/s12879-021-06344-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268612PMC
July 2021

Letter to the Editor Regarding "Long-Term Outcomes Following Lumbar Microendoscopic Decompression for Lumbar Spinal Stenosis With and Without Degenerative Spondylolisthesis: Minimum 10-Year Follow-Up".

World Neurosurg 2021 Jul;151:323-325

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, Sichuan, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.wneu.2021.03.042DOI Listing
July 2021
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