Publications by authors named "Hao Wang"

5,008 Publications

  • Page 1 of 1

Exome sequencing as the first-tier test for pediatric respiratory diseases: a single-center study.

Hum Mutat 2021 May 3. Epub 2021 May 3.

Beijing Key Laboratory for Genetics of Birth Defects, Beijing Pediatric Research Institute; MOE Key Laboratory of Major Diseases in Children; Genetics and Birth Defects Control Center, Beijing Children's Hospital, Capital Medical University; National Center for Children's Health, Beijing, 100045, China.

The high clinical and genetic heterogeneity makes it difficult to reach a confirmative diagnosis of suspected pediatric respiratory inherited diseases. Many patients with monogenic respiratory disorders could be missed without genetic testing. We performed a single-center study in Beijing Children's Hospital to demonstrate the clinical utility of exome sequencing (ES) as a first-tier test by evaluating the diagnostic yields of ES for inherited diseases with respiratory symptoms. A total of 107 patients were recruited in this study. We identified 51 pathogenic or likely pathogenic variants in 37 patients by ES (with or without copy number variants sequencing). The overall diagnostic yield was 34.6% (37/107). The most frequent disorders in our cohort were primary immunodeficiency disease (PIDs) (18/37, 48.6%) and primary ciliary dyskinesia (PCD) (9/37, 24.3%). We further reviewed the directive outcomes of genetic testing on the 37 positive cases. Our study demonstrated the effectiveness of ES as a first-tier test in China for diagnosing monogenic diseases of the respiratory system. In the era of precision medicine, ES as a first-tier test can rapidly make a molecular diagnosis and direct the intervention of the positive cases in pediatric respiratory medicine. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/humu.24216DOI Listing
May 2021

Full wafer scale electroluminescence properties of AlGaN-based deep ultraviolet LEDs with different well widths.

Opt Lett 2021 May;46(9):2111-2114

Deep ultraviolet (DUV) LEDs have great potential in sterilization, water, air purification, and other fields. In this work, DUV LED wafers with different quantum well (QW) widths were grown by metal-organic chemical vapor deposition. It is found that the light output power (LOP) and peak wavelength of all chips are not only related to the QW thickness but also affected by warpage. For the first time, to the best of our knowledge, a positive correlation between the LOP and peak wavelength of DUV LED chips on the same wafer was observed, which is very important for improving the yield of DUV LEDs and reducing costs. Furthermore, the influence of QW thickness on the external quantum efficiency (EQE) of DUV LED has also been investigated. As the thickness of the QW increases, the exciton localization effect decreases and the quantum confinement Stark effect increases. Consequently, DUV LED wafers with a QW thickness of 2 nm have the highest EQE and yield. These findings not only help to improve the efficiency of DUV LEDs but also provide new insights for evaluating the performance of DUV LED wafers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.423264DOI Listing
May 2021

ANPrAod: Identify Antioxidant Proteins by Fusing Amino Acid Clustering Strategy and -Peptide Combination.

Comput Math Methods Med 2021 8;2021:5518209. Epub 2021 Apr 8.

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot 010070, China.

Antioxidant proteins perform significant functions in disease control and delaying aging which can prevent free radicals from damaging organisms. Accurate identification of antioxidant proteins has important implications for the development of new drugs and the treatment of related diseases, as they play a critical role in the control or prevention of cancer and aging-related conditions. Since experimental identification techniques are time-consuming and expensive, many computational methods have been proposed to identify antioxidant proteins. Although the accuracy of these methods is acceptable, there are still some challenges. In this study, we developed a computational model called ANPrAod to identify antioxidant proteins based on a support vector machine. In order to eliminate potential redundant features and improve prediction accuracy, 673 amino acid reduction alphabets were calculated by us to find the optimal feature representation scheme. The final model could produce an overall accuracy of 87.53% with the ROC of 0.7266 in five-fold cross-validation, which was better than the existing methods. The results of the independent dataset also demonstrated the excellent robustness and reliability of ANPrAod, which could be a promising tool for antioxidant protein identification and contribute to hypothesis-driven experimental design.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5518209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049822PMC
April 2021

Role of HEART score in evaluating clinical outcomes among emergency department patients with different ethnicities.

J Int Med Res 2021 Apr;49(4):3000605211010638

Department of Emergency Medicine, JPS Health Network, 1500 S. Main St., Fort Worth, TX, USA.

Objective: We aimed to examine the role of the HEART (history, EKG, age, risk factors, and troponin) score in the evaluation of six clinical outcomes among three groups of patients in the emergency department (ED).

Methods: We performed a retrospective observational study among three ED patient groups including White, Black, and Hispanic patients. ED providers used the HEART score to assess the need for patient hospital admission and for emergent cardiac imaging tests (CITs). HEART scores were measured using classification accuracy rates. Performance accuracies were measured in terms of HEART score in relation to four clinical outcomes (positive findings of CITs, ED returns, hospital readmissions, and 30-day major adverse cardiac events [MACE]).

Results: A high classification accuracy rate (87%) was found for use of the HEART score to determine hospital admission. HEART scores showed moderate accuracy (area under the receiver operating characteristic curve 0.66-0.78) in predicting results of emergent CITs, 30-day hospital readmissions, and 30-day MACE outcomes.

Conclusions: Providers adhered to use of the HEART score to determine hospital admission. The HEART score may be associated with emergent CIT findings, 30-day hospital readmissions, and 30-day MACE outcomes, with no differences among White, Black, and Hispanic patient populations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/03000605211010638DOI Listing
April 2021

Ultra-Stable Potassium Ion Storage of Nitrogen-Doped Carbon Nanofiber Derived from Bacterial Cellulose.

Nanomaterials (Basel) 2021 Apr 27;11(5). Epub 2021 Apr 27.

Guangdong Provincial Key Laboratory of Micro/Nano Optomechatronics Engineering, College of Mechatronics and Control Engineering, Shenzhen University, Shenzhen 518060, China.

As a promising energy storage system, potassium (K) ion batteries (KIBs) have received extensive attention due to the abundance of potassium resource in the Earth's crust and the similar properties of K to Li. However, the electrode always presents poor stability for K-ion storage due to the large radius of K-ions. In our work, we develop a nitrogen-doped carbon nanofiber (N-CNF) derived from bacterial cellulose by a simple pyrolysis process, which allows ultra-stable K-ion storage. Even at a large current density of 1 A g, our electrode exhibits a reversible specific capacity of 81 mAh g after 3000 cycles for KIBs, with a capacity retention ratio of 71%. To investigate the electrochemical enhancement performance of our N-CNF, we provide the calculation results according to density functional theory, demonstrating that nitrogen doping in carbon is in favor of the K-ion adsorption during the potassiation process. This behavior will contribute to the enhancement of electrochemical performance for KIBs. In addition, our electrode exhibits a low voltage plateau during the potassiation-depotassiation process. To further evaluate this performance, we calculate the "relative energy density" for comparison. The results illustrate that our electrode presents a high "relative energy density", indicating that our N-CNF is a promising anode material for KIBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano11051130DOI Listing
April 2021

Assessment of Response to Moderate and High Dose Supplementation of Astaxanthin in Laying Hens.

Animals (Basel) 2021 Apr 16;11(4). Epub 2021 Apr 16.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

In this study, we evaluated the impact of moderate and high dose dietary supplementation of astaxanthin on production performance, quality of eggs, and health status of laying hens. The experiment involved 480 laying hens, divided into four groups of eight replicates. The different groups named A1, A2, A3, and A4 were allocated the same diet supplemented with powder to provide 0, 21.3, 42.6, and 213.4 mg of astaxanthin per kilogram of feed, respectively. One-way ANOVA and linear and quadratic regression analysis were used to assess the differences between the groups. The results showed that the production performance of laying hens and the physical quality of eggs did not significantly differ between the groups ( > 0.05). Astaxanthin distribution in tissues was typical per bird, whereas the egg yolk coloration and astaxanthin concentration increased with the supplementation dose ( < 0.001). However, there was a decrease in concentration and coloration efficacy of astaxanthin at high dose supplementation (213.4 mg/kg) compared to moderate doses (21.3 and 42.6 mg/kg). Blood biochemical tests showed some discrepancies that were not ascribed to the effect of diets, and the increase in liver weight in the A4 group compared to others was equated with an adaptation of laying hens to the high dose supplementation. Astaxanthin improved superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and diminished malondialdehyde (MDA) content in both liver and serum; meanwhile, the activities of SOD and GSH-Px in serum were similar between the moderate doses and high dose supplementation. Additionally, astaxanthin alleviated interleukin 2, 4, and 6 (IL-2, IL-4, and IL-6, respectively) in serum, showing the best effect in A3 and A4 groups. Besides, immunoglobulin G and M (IgG and IgM), as well as tumor necrosis factor-alpha and beta (TNF-α and TNF-β), were not much affected. It was concluded that although astaxanthin has no obvious adverse effect on the performance and health status of laying hens, it may not be valuable for egg fortification and health status improvement of laying hens at high dose supplementation. The high dose astaxanthin supplementation up to 213.4 mg/kg in the diet might be avoided.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ani11041138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071492PMC
April 2021

Induced Torpor as a Countermeasure for Low Dose Radiation Exposure in a Zebrafish Model.

Cells 2021 Apr 14;10(4). Epub 2021 Apr 14.

School of Biological Sciences & Institute for Global Food Security, Queens University Belfast, Belfast BT9 5DL, UK.

The development of the Artemis programme with the goal of returning to the moon is spurring technology advances that will eventually take humans to Mars and herald a new era of interplanetary space travel. However, long-term space travel poses unique challenges including exposure to ionising radiation from galactic cosmic rays and potential solar particle events, exposure to microgravity and specific nutritional challenges arising from earth independent exploration. Ionising radiation is one of the major obstacles facing future space travel as it can generate oxidative stress and directly damage cellular structures such as DNA, in turn causing genomic instability, telomere shortening, extracellular-matrix remodelling and persistent inflammation. In the gastrointestinal tract (GIT) this can lead to leaky gut syndrome, perforations and motility issues, which impact GIT functionality and affect nutritional status. While current countermeasures such as shielding from the spacecraft can attenuate harmful biological effects, they produce harmful secondary particles that contribute to radiation exposure. We hypothesised that induction of a torpor-like state would confer a radioprotective effect given the evidence that hibernation extends survival times in irradiated squirrels compared to active controls. To test this hypothesis, a torpor-like state was induced in zebrafish using melatonin treatment and reduced temperature, and radiation exposure was administered twice over the course of 10 days. The protective effects of induced-torpor were assessed via RNA sequencing and qPCR of mRNA extracted from the GIT. Pathway and network analysis were performed on the transcriptomic data to characterise the genomic signatures in radiation, torpor and torpor + radiation groups. Phenotypic analyses revealed that melatonin and reduced temperature successfully induced a torpor-like state in zebrafish as shown by decreased metabolism and activity levels. Genomic analyses indicated that low dose radiation caused DNA damage and oxidative stress triggering a stress response, including steroidal signalling and changes to metabolism, and cell cycle arrest. Torpor attenuated the stress response through an increase in pro-survival signals, reduced oxidative stress via the oxygen effect and detection and removal of misfolded proteins. This proof-of-concept model provides compelling initial evidence for utilizing an induced torpor-like state as a potential countermeasure for radiation exposure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cells10040906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071006PMC
April 2021

Landscape Pattern Evolution Processes of Wetlands and Their Driving Factors in the Xiong'an New Area of China.

Int J Environ Res Public Health 2021 Apr 21;18(9). Epub 2021 Apr 21.

College of Water Science and Engineering, Zhengzhou University, Zhengzhou 450001, China.

Wetland landscape patterns are the result of various ecological and hydrological processes. Based on the land use landscape types from 1980 to 2017, a transfer matrix, landscape pattern analysis index, and principal component analysis were used to analyze the landscape pattern evolution in the Xiong'an New Area of China, which has a large area with a lake and river wetlands. The results showed that the wetland area has changed greatly since 2000 and the beach land has decreased greatly, while the area of the lake and river wetlands has increased slightly. Beach land was the dominant landscape type of the wetland. The dominant degree of the wetland landscape showed a slightly decreasing trend, and the patches tended to be scattered. The shape complexity of the ponds was the lowest, while that of rivers was the highest. The fragmentation degree of the wetland patches increased, the proportion of landscape types tended to be equalized, and the landscape heterogeneity increased. The leading factors of the wetland landscape change can be summarized as socioeconomic, meteorological, and hydrological processes, with a cumulative contribution rate of 85.3%, among which socioeconomic development was the most important factor. The results have important guiding significance for the ecological restoration and management of wetlands in the Xiong'an New Area and other wetland ecosystems with rivers and lakes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph18094403DOI Listing
April 2021

Inhibition of Cochlear HMGB1 Expression Attenuates Oxidative Stress and Inflammation in an Experimental Murine Model of Noise-Induced Hearing Loss.

Cells 2021 Apr 5;10(4). Epub 2021 Apr 5.

Department of Otolaryngology-Head and Neck Surgery, National Defense Medical Center, Tri-Service General Hospital, Taipei 11490, Taiwan.

Noise-induced hearing loss (NIHL) is a common inner ear disease but has complex pathological mechanisms, one of which is increased oxidative stress in the cochlea. The high-mobility group box 1 (HMGB1) protein acts as an inflammatory mediator and shows different activities with redox modifications linked to the generation of reactive oxygen species (ROS). We aimed to investigate whether manipulation of cochlear HMGB1 during noise exposure could prevent noise-induced oxidative stress and hearing loss. Sixty CBA/CaJ mice were divided into two groups. An intraperitoneal injection of anti-HMGB1 antibodies was administered to the experimental group; the control group was injected with saline. Thirty minutes later, all mice were subjected to white noise exposure. Subsequent cochlear damage, including auditory threshold shifts, hair cell loss, expression of cochlear HMGB1, and free radical activity, was then evaluated. The levels of HMGB1 and 4-hydroxynonenal (4-HNE), as respective markers of reactive nitrogen species (RNS) and ROS formation, showed slight increases on post-exposure day 1 and achieved their highest levels on post-exposure day 4. After noise exposure, the antibody-treated mice showed markedly less ROS formation and lower expression of NADPH oxidase 4 (NOX4), nitrotyrosine, inducible nitric oxide synthase (iNOS), and intercellular adhesion molecule-1 (ICAM-1) than the saline-treated control mice. A significant amelioration was also observed in the threshold shifts of the auditory brainstem response and the loss of outer hair cells in the antibody-treated versus the saline-treated mice. Our results suggest that inhibition of HMGB1 by neutralization with anti-HMGB1 antibodies prior to noise exposure effectively attenuated oxidative stress and subsequent inflammation. This procedure could therefore have potential as a therapy for NIHL.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cells10040810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066810PMC
April 2021

Amplifying Effect of Chronic Lisinopril therapy on Diastolic Function and the Angiotensin-(1-7) Axis by the G1 Agonist in Ovariectomized Spontaneously Hypertensive Rats.

Transl Res 2021 Apr 26. Epub 2021 Apr 26.

Department of Anesthesiology, Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, NC, 27157, USA; Department of Internal Medicine-Molecular Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, NC, 27157, USA. Electronic address:

GPER activation by G1 attenuates diastolic dysfunction from estrogen loss, which may be partly due to suppression of angiotensin II pathological actions. We aimed to determine the independent effects of 8 weeks of G1 (100 µg/kg/day, subcutaneous pellet), ACE-inhibition (ACEi; lisinopril 10 mg/kg, drinking water), or combination therapy versus vehicle in the ovariectomized (OVX) spontaneously hypertensive rat (SHR) on cardiac function and morphometrics (echocardiography), serum equilibrium of angiotensins (mass spectroscopy) and cardiac components of the RAS (Western blotting). G1 alone and when combined with ACEi enhanced myocardial relaxation (e´: 30 and 17%) and diastolic wall strain (DWS: 76 and 68%) while reducing relative wall thickness (RWT: 20 and 33%) and filling pressures (E/e´: 30 and 37%). Cardiac expression levels of Mas receptor (Mas-R) and ACE2 also increased in the presence of G1. Strong antihypertensive effects of lisinopril monotherapy were associated with reductions in RWT, collagen deposition and E/e´ without overtly altering e´ or DWS. Chronic ACEi also increased cardiac levels of Mas-R and AT-R and tilted the circulating RAS toward the formation of Ang-(1-7), which was amplified in the presence of G1. In vitro studies further revealed that an inhibitor to prolyl endopeptidase (PEP), but not to neprilysin, significantly reduced serum Ang-(1-7) levels in G1-treated rats, suggesting that G1 might be increasing Ang-(1-7) formation via PEP. We conclude that activating GPER with G1 augments components of the cardiac RAS and improves diastolic function without lowering blood pressure, and that lisinopril-induced blood pressure control and cardiac alterations in OVX SHR are permissive in facilitating G1 to augment Ang-(1-7) in serum, thereby strengthening its cardioprotective benefits.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.trsl.2021.04.004DOI Listing
April 2021

Potential risk analysis and experience summarization of unstable factors of cranial fixation devices in neurosurgical operations: three-case reports and systematic review.

Chin Neurosurg J 2021 Apr 28;7(1):25. Epub 2021 Apr 28.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: The use of cranial fixation devices in neurosurgery is very common, which is considered to be an important auxiliary method for many craniotomies. However, previous studies have reported complications of using cranial fixation devices, including brain tissue, nerve and blood vessel damage, scalp laceration, subcutaneous hematoma, etc. Some of the complications are serious and even potentially fatal, and the causes of which may be related to the incorrect use of cranial fixation devices. Although there are no serious complications in our review, the cause of that needs to be further summarized and analyzed, as so to minimize the serious consequences caused by the cranial fixation device slippage and ensure the safety of the patients' surgical procedure.

Case Presentation: In our recent work, we have continuously found three cases of unstable cranial fixation devices, which make us to analyze the possible factors and summarize experience combined with the review of other senior neurosurgeons (more than 3 years of working experience) from different departments of neurosurgery.

Conclusions: Based on our recent incidents of unstable cranial fixation and the experience of investigating and analyzing senior doctors from different neurosurgery centers, we summarized experience to minimize the risk of unstable cranial fixation. We tried a variety of options, including a safe anatomical location for cranial fixation, teamwork, and communication with anesthesiologists and itinerant nurses, to ensure the stability of the patient's cranial fixation devices. The data obtained in this survey has great limitations, including the doctor's personal prejudice and dependence on anecdotal memories. Therefore, the data should be interpreted with caution. However, there are still some modes that can help to better understand the use of safe cranial fixation. Based on the above research and analysis, we have made recommendations that may help neurosurgeons to avoid preventable complications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s41016-021-00244-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080414PMC
April 2021

Inhibition of Syk promotes chemical reprogramming of fibroblasts via metabolic rewiring and H S production.

EMBO J 2021 Apr 28:e106771. Epub 2021 Apr 28.

The MOE Key Laboratory of Biosystems Homeostasis & Protection and Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China.

Chemical compounds have recently been introduced as alternative and non-integrating inducers of pluripotent stem cell fate. However, chemical reprogramming is hampered by low efficiency and the molecular mechanisms remain poorly characterized. Here, we show that inhibition of spleen tyrosine kinase (Syk) by R406 significantly promotes mouse chemical reprogramming. Mechanistically, R406 alleviates Syk / calcineurin (Cn) / nuclear factor of activated T cells (NFAT) signaling-mediated suppression of glycine, serine, and threonine metabolic genes and dependent metabolites. Syk inhibition upregulates glycine level and downstream transsulfuration cysteine biosynthesis, promoting cysteine metabolism and cellular hydrogen sulfide (H S) production. This metabolic rewiring decreased oxidative phosphorylation and ROS levels, enhancing chemical reprogramming. In sum, our study identifies Syk-Cn-NFAT signaling axis as a new barrier of chemical reprogramming and suggests metabolic rewiring and redox homeostasis as important opportunities for controlling cell fates.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.15252/embj.2020106771DOI Listing
April 2021

Venlafaxine Inhibits the Apoptosis of SHSY-5Y Cells Through Active Wnt/β-Catenin Signaling Pathway.

Neuropsychiatr Dis Treat 2021 21;17:1145-1151. Epub 2021 Apr 21.

Department of Psychological Medicine, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.

Objective: This study aimed to explore the mechanism of venlafaxine in regulating the apoptosis of SHSY-5Y cells induced by hypoxia.

Methods: The CoCl2-induced neuronal hypoxia model was established based on SHSY-5Y cells. The morphology and related protein expression of SHSY-5Y cells were detected by qPCR, ELISA and Western blot.

Results: Under the condition of hypoxia-induced by CoCl2, the expression of HIF-1α in SHSY-5Y cells was up-regulated and the expression of β-catenin was down-regulated. After adding siRNA targeting HIF-1 α to the culture cell system, down-regulation of β -catenin expression in SHSY-5Y cells was restored. This confirmed the existence of the "hypoxia-HIF-1α-Wnt/β-catenin-depression" axis. Further studies have shown that venlafaxine can alleviate neuronal apoptosis induced by hypoxia by upregulating the Wnt/β-catenin signaling pathway.

Conclusion: Venlafaxine regulates apoptosis induced by hypoxia through the Wnt/β-catenin signaling pathway, which provides a new theoretical basis for the treatment of depression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/NDT.S294998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071209PMC
April 2021

Moxibustion therapy for treating patients with postpartum urinary retention: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(17):e25683

Beijing University of Chinese Medicine, Chaoyang, Beijing, China.

Background: Postpartum urinary retention (PUR) is one of the most common complications after parturition which affect women's recovery after childbirth. Many clinical trials have shown that moxibustion, a traditional Chinese medicine therapy, is effective in treating PUR. But its effectiveness has not been evaluated scientifically and systematically. Therefore, this review aims to evaluate the safety and effectiveness of moxibustion therapy in treating patients with PUR.

Methods: We will search the following electronic databases, regardless of publication status and languages, from their respective inception dates to February 2021: the Cochrane Central Register of Controlled Trails, Pubmed, EMBASE, China National Knowledge Infrastructure, Chinese Biomedical Literature Database, Chinese Scientific Journal Database, and Wan-Fang Database. Clinical randomized controlled trials (RCTs) related to moxibustion therapy for treating PUR will be included. Study selection, data collection, and quality assessment will be independently conducted by 2 researchers. For data synthesis, we will select either the fixed-effects or random-effects model according to heterogeneity assessment. Cure rates and postvoid residual volume (PVRV) will be the primary outcomes. The total effective rate and first urination time will be the second outcomes. Review Manager Software (RevMan) V.5.3 will be used if it is appropriate for meta-analysis. Otherwise, a systematic narrative synthesis will be conducted. The results will be presented as risk ratio (RR) with 95% confidence interval (CI) for dichotomous data and weight mean difference (WMD) or standard mean difference (SMD) 95% CI for continuous data.

Trial Registration Number: INPLASY 202140037.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084072PMC
April 2021

Prognostic Value of Angiopoietin-Like 4 in Patients with Acute Respiratory Distress Syndrome.

Shock 2021 Jan 20. Epub 2021 Jan 20.

*Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, and Department of Respiratory and Critical Care Medicine, West China Hospital of Sichuan University. Guoxuexiang 37, Chengdu, Sichuan 610041, China †Department of Critical Care Medicine, Haikou Municipal Hospital, and Central South University, Xiangya School of Medicine Affiliated Haikou Hospital.

Background: Angiopoietin-like 4 (ANGPTL4) is a secreted glycoprotein that plays an important role in endothelial injury and the inflammatory response. Experimental models have implicated ANGPTL4 in acute respiratory distress syndrome (ARDS), but its impact on the progression of ARDS is unclear.

Methods: Paired bronchoalveolar lavage fluid (BALF) and serum samples were obtained from patients with ARDS (n = 56) within 24 h of diagnosis and from control subjects (n = 32). ANGPTL4, angiopoietin-2, interleukin (IL)-6 and tumor necrosis factor (TNF)-α levels were measured by magnetic Luminex assay. BALF albumin (BA) and serum albumin (SA) were evaluated by enzyme-linked immunosorbent assay.

Results: BALF and serum ANGPTL4 concentrations were higher in patients with ARDS than in controls and were even higher in survivors than in non-survivors. The serum ANGPTL4 level was higher in indirect (extrapulmonary) ARDS than in direct (pulmonary) ARDS. Furthermore, BALF and serum ANGPTL4 levels correlated well with angiopoietin-2, IL-6, and TNF-α levels in BALF and serum. BALF ANGPTL4 was positively correlated with the BA/SA ratio (an indicator of pulmonary vascular permeability), and serum ANGPTL4 was associated with the severity of multiple organ dysfunction syndrome based on SOFA and APACHE II scores. Moreover, serum ANGPTL4 was better able to predict 28-day ARDS-related mortality (AUC 0.746, p < 0.01) than the APACHE II score or PaO2/FiO2 ratio. Serum ANGPTL4 was identified as an independent risk factor for mortality in a univariate Cox regression model (p < 0.001).

Conclusion: ANGPTL4 levels were elevated in patients with ARDS and significantly correlated with disease severity and mortality. ANGPTL4 may be a novel prognostic biomarker in ARDS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/SHK.0000000000001734DOI Listing
January 2021

Biomimetic Ca nanogenerator based on ions interference strategy for tumour-specific therapy.

J Drug Target 2021 May 3:1-8. Epub 2021 May 3.

Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China.

Intracellular Ca ions as second messenger played key role in cell behaviour, which was often overlooked in traditional antitumor treatment. Disrupting Ca ion homeostasis by Ca overload might switch ions signal from 'regulating' to 'destroying'. Inspired by this, a biomimetic Ca nanogenerator was constructed. Briefly, the curcumin (CUR) was loaded into mesoporous calcium carbonate nanoparticles (MCC NPs), and then coated with platelet (PLT) membrane. Upon reaching tumour cells by PLT membrane-mediated tumour targeting effect, PLT@MCC/CUR would instantaneously decompose in acidic lysosomes, concurrently accompanying with Ca generation and CUR release. The CUR could further facilitate Ca release from endoplasmic reticulum (ER) and inhibit Ca efflux, aggravating Ca overload to disrupt mitochondrial Ca homeostasis for mitochondria apoptosis signalling pathway activation. Interestingly, such effect was ineffective in normal cells, realising the tumour-specific therapeutic therapy. Based on ions interference strategy, PLT@MCC/CUR herein offered synergistic combination of Ca overload therapy and chemotherapy, which would pave the way towards more effective nanotherapeutics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/1061186X.2021.1919123DOI Listing
May 2021

RNF217 regulates iron homeostasis through its E3 ubiquitin ligase activity by modulating ferroportin degradation.

Blood 2021 Apr 25. Epub 2021 Apr 25.

Zhejiang University School of Medicine, Hangzhou, China.

Ferroportin (FPN), the body's sole iron exporter, is essential for maintaining systemic iron homeostasis. In response to either increased iron or inflammation, hepatocyte-secreted hepcidin binds to FPN, inducing its internalization and subsequent degradation. However, the E3 ubiquitin ligase that underlies FPN degradation has not been identified. Here, we report the identification and characterization of a novel mechanism involving the RNF217-mediated degradation of FPN. A combination of two different E3 screens revealed that the Rnf217 gene is a target of Tet1, mediating the ubiquitination and subsequent degradation of FPN. Interestingly, loss of Tet1 expression causes an accumulation of FPN and an impaired response to iron overload, manifested by increased iron accumulation in the liver together with decreased iron in the spleen and duodenum. Moreover, we found that the degradation and ubiquitination of FPN could be attenuated by mutating RNF217. Finally, using two conditional knockout mouse lines, we found that knocking out Rnf217 in macrophages increases splenic iron export by stabilizing FPN, whereas knocking out Rnf217 in intestinal cells appears to increase iron absorption. These findings suggest that the Tet1-RNF217-FPN axis regulates iron homeostasis, revealing new therapeutic targets for FPN-related diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1182/blood.2020008986DOI Listing
April 2021

Identification of hotspots of threatened inland fish species and regions for restoration based on longitudinal river connectivity.

J Environ Manage 2021 Apr 21;290:112572. Epub 2021 Apr 21.

State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, Department of Water Resources (DWR), China Institute of Water Resources and Hydropower Research, Beijing, 100038, PR China.

Dams have reduced longitudinal river connectivity (LRC) worldwide, impairing habitat and migration opportunities for many freshwater fish species. However, national assessments linking LRC and threatened inland fish species distributions are lacking. Here, we show the trends in the LRC in China over the past 60 years, and quantitatively analyse their implications for threatened inland fish species. The average LRC in China decreased from 93.5% in 1960 to 25.1% in 2018, and the significant deterioration in LRC occurred over the last 20 years. Water resource regions with a high number of threatened fish species are concentrated in southern China, and the degree of threat to inland fish species increases with a decreasing average LRC. A total of 125 inland fish species are threatened by habitat degradation and loss, which is mainly caused by the construction of dams. Intrinsic factors and pollution are two other key threats to fish species in addition to the reduction of LRC. The results of this study can help us better understand the trends in LRC in China and their implications for inland fishes. Meanwhile, this study provides guidance for river management to help preserve biological diversity, including enhancing management of natural reserve areas, establishing ecological compensation mechanisms, preventing biological invasion, and monitoring and evaluation of restoration efforts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2021.112572DOI Listing
April 2021

A mathematical model for Vibrio-phage interactions.

Math Biosci Eng 2021 Mar;18(3):2688-2712

Department of Mathematical and Statistical Sciences, University of Alberta, Edmonton, Alberta, T6G 2G1, Canada.

A cholera model has been formulated to incorporate the interaction of bacteria and phage. It is shown that there may exist three equilibria: one disease free and two endemic equilibria. Threshold parameters have been derived to characterize stability of these equilibria. Sensitivity analysis and disease control strategies have been employed to characterize the impact of bacteria-phage interaction on cholera dynamics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3934/mbe.2021137DOI Listing
March 2021

Control of light-valley interactions in 2D transition metal dichalcogenides with nanophotonic structures.

Nanoscale 2021 Apr 25;13(13):6357-6372. Epub 2021 Mar 25.

Beijing Computational Science Research Center, Beijing 100193, China.

Electronic valley in two-dimensional transition-metal dichalcogenides (2D TMDCs) offers a new degree of freedom for information storage and processing. The valley pseudospin can be optically encoded by photons with specific helicity, enabling the construction of electronic information devices with both high performance and low power consumption. Robust detection, manipulation and transport of the valley pseudospins at room temperature are still challenging because of the short lifetime of valley-polarized carriers and excitons. Integrating 2D TMDCs with nanophotonic objects such as plasmonic nanostructures provides a competitive solution to address the challenge. The research in this field is of practical interest and can also present rich physics of light-matter interactions. In this minireview, recent progress on using nanophotonic strategies to enhance the valley polarization degree, especially at room temperature, is highlighted. Open questions, major challenges, and interesting future developments in manipulating the valley information in 2D semiconductors with the help of nanophotonic structures will also be discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr08000dDOI Listing
April 2021

Defect Termination in the UiO-66 Family of Metal-Organic Frameworks: The Role of Water and Modulator.

J Am Chem Soc 2021 Apr 22. Epub 2021 Apr 22.

Department of Physics and Center for Functional Materials, Wake Forest University, Winston-Salem, North Carolina 27109, United States.

The defect concentration in the prototypical metal-organic framework UiO-66 can be well controlled during synthesis, leading to precisely tunable physicochemical properties for this structure. However, there has been a long-standing debate regarding the nature of the compensating species present at the defective sites. Here, we present unambiguous spectroscopic evidence that the missing-linker defect sites in an ambient environment are compensated with both carboxylate and water (bound through intermolecular hydrogen bonding), which is further supported by calculations. In contrast to the prevailing assumption that the monocarboxylate groups (COO) of the modulators form bidentate bonding with two Zr sites, COO is found to coordinate to an open Zr site in an unidentate mode. The neighboring Zr site is terminated by a coordinating HO molecule, which helps to stabilize the COO group. This finding not only provides a new understanding of defect termination in UiO-66, but also sheds light on the origin of its catalytic activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.1c01408DOI Listing
April 2021

Double Confined MoO/Sn/NC@NC Nanotubes: Solid-Liquid Synthesis, Conformal Transformation, and Excellent Lithium-Ion Storage.

ACS Appl Mater Interfaces 2021 Apr 22. Epub 2021 Apr 22.

School of Materials Science and Engineering, Central South University, Changsha, Hunan 410083, China.

The rational design of a hollow heterostructure promotes the development of highly durable anode materials for lithium-ion batteries. Herein, carbon-confined MoO/Sn/NC@NC heterostructured nanotubes evolving from MoO nanorods have been successfully synthesized for the first time. In the growth of the Mo/Sn precursor, a peculiar microstructure evolution occurs from solid rods to hollow tubes through a solid-liquid reaction. The MoO/Sn composite is restricted within the double carbon layer after subsequent annealing and carbonization that distinctly inherits the morphology of the Mo/Sn precursor. The resulting electrode shows good capacities with hardly any attenuation (925.4 mA h g after 100 cycles at 100 mA g) and excellent long cycle life (620.1 mA h g after 1000 cycles at 2 A g). The MoO/Sn/NC@NC nanotubes contain the synergistic effect, elaborate core-shell structure, large specific surface areas, and abundant voids. These superiorities not only provide beneficial channels for the electrolyte to fully come into contact with electrode materials and more active sites for redox reactions but also effectively alleviate the volume fluctuation and sustain the electrical connectivity to retain a stable solid-electrolyte interface layer, indeed, bringing about the prominent Li-storage performance. The present study paves a feasible avenue to prepare core-shell structures with high reversible capacity and long-term cycle performance for energy storage devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c21645DOI Listing
April 2021

Urinary exosomal CD26 is associated with recovery from acute kidney injury in intensive care units: a prospective cohort study.

Clin Chem Lab Med 2021 Apr 21. Epub 2021 Apr 21.

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, P. R. China.

Objectives: Currently there is no validated method to predict renal reversal and recovery after acute kidney injury (AKI). As exosomes have the potential for AKI prognosis and CD26 is involved in the mechanisms in AKI, this study aims to investigate whether urinary exosomal CD26 is associated with renal-related outcomes and explore its prospect as a novel prognosis biomarker.

Methods: This was a single-center, prospective cohort study. A total of 133 AKI patients and 68 non-AKI patients admitted to ICU in Qilu Hospital Shandong University from January 2017 to January 2018. Urine samples were collected at enrollment and the relative expression of CD26 (CD26 percentage) in urinary exosomes was examined, that was then categorized into a low-CD26 level and a high-CD26 level.

Results: CD26 percentage was significantly lower in the AKI cohort than in the control cohort. Within the AKI cohort, a high-CD26 level was associated with lower incidence of major adverse kidney events within 90 days, but higher incidence of reversal within 28 days. In AKI survivors, a high-CD26 level had a 4.67-, 3.50- and 4.66-fold higher odds than a low-CD26 level for early reversal, recovery and reversal, respectively, after adjustment for clinical factors. Prediction performance was moderate for AKI survivors but improved for non-septic AKI survivors.

Conclusions: Urinary exosomal CD26 is associated with renal reversal and recovery from AKI and is thus a promising prognosis biomarker.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1515/cclm-2021-0040DOI Listing
April 2021

Chemical reactions trigger peptide self-assembly in vivo for tumor therapy.

ChemMedChem 2021 Apr 21. Epub 2021 Apr 21.

National Center for Nanoscience and Technology, No. 11 Beiyitiao, Zhongguancun, 100190, Beijing, CHINA.

Self-assembly peptide materials have promoted the development of science research including life science, optics, medicine, and catalysis in the past two decades. Especially in tumor treatment, peptide self-assembly strategies have exhibited promising potentials by the incomparable bio-compatibility, construction modularization and structure controllability diversity. Driven by physical and chemical trigger factors, peptide can self-assemble  in vivo  to form fiber, sphere, hydrogel, or ribbon to achieve predeterminate biological functions. To note, peptide self-assembly triggered by chemical reactions provides superior specificity and intelligent responsiveness to produce assembly-induced biological effects in target regions. Here from the perspective of triggers of peptide assembly, we briefly reviewed the applications of  in vivo  peptide self-assembly strategies for tumor treatment including tumor pathology factors induced chemical reactions and bio-orthogonal reactions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cmdc.202100254DOI Listing
April 2021

Hydrogel composite of lanthanum and Halorubrum ejinoor sp. cell lysate as an adsorbing material.

Biotechnol Lett 2021 Apr 20. Epub 2021 Apr 20.

Academic Support Center, Kogakuin University, Hachioji, Tokyo, 192-0015, Japan.

Objectives: Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles. This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp. (H.e.) which was isolated from a saline lake in Inner Mongolia, China. Furthermore, formation mechanism and potential applications of the hydrogel as an adsorbing material are discussed.

Results: Halorubrum ejinoor sp. (H.e.) cell lysate was firstly prepared by adding pure water onto the H.e. cell pellet, followed by a short incubation at 60 °C. The cell lysate was injected into different metal ion (or H) solutions to obtain the hydrogel composite. It was observed that H, Fe, La, Cu, and Ca induced gelation of the cell lysate, while Fe, Co, Ni, Mg, Na, and K did not. DNA and extracellular polysaccharides (EPS) in the H.e. cell lysate were found to be responsible for the gelation reaction. These results suggest that DNA and EPS should be crosslinked by metal ions (or H) and form a networked structure in which the metal ion (or H) serves as an anchor point. Potential application of the hydrogel as an adsorbing material was explored using La-induced H.e. hydrogel composite. The hydrogel composite can adsorb the fluoride, phosphate and DNA-binding carcinogenic agents, such as acridine orange.

Conclusions: The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material. This work is expected to expand the technical applications of haloarchaea.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-021-03132-yDOI Listing
April 2021

Hydrogen versus tetrel bonds in complexes of 3-oxetanone with water and formaldehyde.

Phys Chem Chem Phys 2021 Mar 22;23(12):7295-7301. Epub 2021 Mar 22.

School of Chemistry and Chemical Engineering, Chongqing University, No. 55 Daxuecheng South Rd, Shapingba, Chongqing 401331, China.

The ability and preference of 3-oxetanone to form hydrogen or tetrel bonds have been investigated in its complexes with water and formaldehyde by using Fourier transform microwave spectroscopy complemented with quantum chemical calculations. Different types of interactions and internal dynamics have been observed in the targeted complexes. With water, the ether oxygen of 3-oxetanone is the favoured interaction site forming a classical O-HO hydrogen bond. Quite differently, the carbonyl group of 3-oxetanone plays the dual role as a tetrel donor and a proton acceptor in the 3-oxetanone-formaldehyde complex, featuring the CO tetrel bond and C-HO weak hydrogen bond interactions. Splittings originated from the internal rotation of formaldehyde around its C axis were also observed. The V barrier was estimated to be 375(10) cm based on Meyer's one-dimensional flexible model. The changes in geometries and electronic densities upon complexation would shed light on the impact of archetype solvent and organic substrate molecules on the reactivity of 3-oxetanone.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cp00239bDOI Listing
March 2021

Competitive and cooperative n →π* and n →σ* interactions in benzaldehyde-formaldehyde: rotational characterization.

Phys Chem Chem Phys 2021 Apr 30;23(14):8778-8783. Epub 2021 Mar 30.

School of Chemistry and Chemical Engineering, Chongqing University, Daxuecheng South Rd. 55, 401331, Chongqing, China.

The rotational spectrum of the 1 : 1 benzaldehyde-formaldehyde complex has been investigated by pulsed jet Fourier transform microwave spectroscopy combined with ab initio calculations. The two most stable isomers were observed, with the relative abundance ratio N/N≈ 3/1 estimated with intensity measurements. Both observed isomers are stabilized by one dominating O[double bond, length as m-dash]CO tetrel bond (n →π* interaction) and one secondary C-HO hydrogen bond. Natural bond orbital analysis and electron localization function analysis were applied to characterize the nature of the noncovalent interactions in the target complex.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cp06409bDOI Listing
April 2021

Nimodipine inhibits intestinal and aortic smooth muscle contraction by regulating Ca-activated Cl channels.

Toxicol Appl Pharmacol 2021 Apr 16;421:115543. Epub 2021 Apr 16.

Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, School of Life Sciences, Liaoning Normal University, Dalian 116000, PR China. Electronic address:

Nimodipine is a clinically used dihydropyridine L-type calcium channel antagonist that effectively inhibits transmembrane Ca influx following the depolarization of smooth muscle cells, but the detailed effect on smooth muscle contraction is not fully understood. Ca-activated Cl channels (CaCCs) in vascular smooth muscle cells (VSMCs) may regulate vascular contractility. We found that nimodipine can inhibit transmembrane protein 16A (TMEM16A) activity in a concentration-dependent manner by cell-based fluorescence-quenching assay and short-circuit current analysis, with an IC value of ~5 μM. Short-circuit current analysis also showed that nimodipine prevented Ca-activated Cl current in both HT-29 cells and mouse colonic epithelia accompanied by significantly decreased cytoplasmic Ca concentrations. In the absence of extracellular Ca, nimodipine still exhibited an inhibitory effect on TMEM16A/CaCCs. Additionally, the application of nimodipine to CFTR-expressing FRT cells and mouse colonic mucosa resulted in mild activation of CFTR-mediated Cl currents. Nimodipine inhibited basolateral CCh-activated K channel activity with no effect on Na/K-ATPase activity. Evaluation of intestinal smooth muscle contraction showed that nimodipine inhibits intestinal smooth muscle contractility and frequency, with an activity pattern that was similar to that of non-specific inhibitors of CaCCs. In aortic smooth muscle, the expression of TMEM16A in thoracic aorta is higher than that in abdominal aorta, corresponding to stronger maximum contractility in thoracic aorta smooth muscle stimulated by phenylephrine (PE) and E. Nimodipine completely inhibited the contraction of aortic smooth muscle stimulated by E, and partially inhibited the contraction stimulated by PE. In summary, the results indicate that nimodipine effectively inhibits TMEM16A/CaCCs by reduction transmembrane Ca influx and directly interacting with TMEM16A, explaining the mechanisms of nimodipine relaxation of intestinal and aortic smooth muscle contraction and providing new targets for pharmacological applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.taap.2021.115543DOI Listing
April 2021

Effects of dietary polyphenol supplementation on iron status and erythropoiesis: a systematic review and meta-analysis of randomized controlled trials.

Am J Clin Nutr 2021 Apr 19. Epub 2021 Apr 19.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing, China.

Background: The iron-chelating activities of polyphenols raise concern whether there is a risk of iron deficiency or anemia induced by polyphenol supplementation. Results from clinical trials regarding the effects of polyphenol supplementation on iron status and erythropoiesis are inconclusive.

Objective: We performed a systematic review and meta-analysis of randomized controlled trials to determine the effects of polyphenol supplementation on iron status and erythropoiesis.

Methods: Published articles were searched between May 1988 and 7 December, 2020. Finally, we identified 34 randomized controlled trials. Random-effects meta-analyses were performed to obtain the weighted mean difference of serum iron (SI), transferrin saturation (TS), ferritin, and hemoglobin concentration. Funnel plots and Egger's test were used to determine the risk of bias. The robustness of the effect sizes was examined by sensitivity analysis.

Results: Polyphenol supplementation had an inhibitory effect on the SI concentration (-13.72 μg/dL; 95% CI: -20.74, -6.71) and TS (-3.10%; 95% CI: -4.93, -1.27), with no effect on ferritin (-9.34 ng/mL; 95% CI: -28.55, 9.87). Polyphenols increased the hemoglobin concentration (8.53 g/L; 95% CI: 3.33, 13.73). In healthy participants, polyphenol reduced the TS (-3.83%; 95% CI: -7.47, -0.19) and increased the hemoglobin concentration (12.87 g/L; 95% CI: 1.61, 24.14). Similarly, polyphenol reduced the SI concentration (-8.60 μg/dL; 95% CI: -16.10, -1.10) and increased the hemoglobin concentration (8.50 g/L; 95% CI: 0.86, 16.15) in patients with metabolic diseases. In patients with β-thalassemia, polyphenol decreased the SI concentration (-23.19 μg/dL; 95% CI: -35.84, -10.55), TS (-3.23%; 95% CI: -5.54, -0.91), and ferritin concentration (-223.62 ng/mL; 95% CI: -359.32, -87.91), but had no effect on the hemoglobin concentration.

Conclusion: Healthy individuals and patients with metabolic diseases may benefit from the positive impact of polyphenols on erythropoiesis. Patients with β-thalassemia may benefit from the effect of polyphenols on reducing SI. This trial was registered at PROSPERO (International prospective register of systematic reviews) as CRD42020161983.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/ajcn/nqab068DOI Listing
April 2021

Conformational Transition-Triggered Disassembly of Therapeutic Peptide Nanomedicine for Tumor Therapy.

Adv Healthc Mater 2021 Apr 18:e2100333. Epub 2021 Apr 18.

CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Haidian District, Beijing, 100190, P. R. China.

Cationic therapeutic peptides have received widespread attention due to their excellent antibacterial and antitumor properties. However, most of these peptides have undesirable delivery efficiency and high hemolytic toxicity due to the positively charged α-helix structure containing many lysine and arginine, which may restrict its in vivo applications. Herein, a conformationally transformed therapeutic peptide Pep-HCO modified with bicarbonates on guanidine groups is designed. Such a design allows Pep-HCO ((nap-RAGLQFPVGRLLRRLLRRLLR) nHCO ) to self-assemble into nanoparticles (NP-Pep) due to disrupting helix folding and the formation of intermolecular hydrogen bonding between bicarbonates and guanidine groups. When pH is from 7.4 to 6.5 at the tumor sites, guanidine bicarbonate can be hydrolyzed to form CO and guanidine groups, resulting in the disassembling of the NP-Pep into monomers α-Pep with a positively charged α-helix structure. In vivo, NP-Pep not only inhibits the tumor growth of xenografted mice with a twofold enhanced inhibition rate compared with α-Pep treatment group, but also significantly reduces the hemolytic toxicity by responding to the pH of tumor microenvironment. Therefore, the strategy of conformational transition-triggered disassembly of nanoparticles allows efficient delivery of cationic therapeutic peptides and lowering the hemolytic toxicity, which may provide an avenue for developing high-performance cationic peptide in vivo applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adhm.202100333DOI Listing
April 2021