Publications by authors named "Hao Song"

391 Publications

METTL3-mediated mA RNA methylation promotes the anti-tumour immunity of natural killer cells.

Nat Commun 2021 Sep 17;12(1):5522. Epub 2021 Sep 17.

Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230027, China.

Natural killer (NK) cells exert critical roles in anti-tumor immunity but how their functions are regulated by epitranscriptional modification (e.g., N-methyladenosine (mA) methylation) is unclear. Here we report decreased expression of the mA "writer" METTL3 in tumor-infiltrating NK cells, and a positive correlation between protein expression levels of METTL3 and effector molecules in NK cells. Deletion of Mettl3 in NK cells alters the homeostasis of NK cells and inhibits NK cell infiltration and function in the tumor microenvironment, leading to accelerated tumor development and shortened survival in mice. The gene encoding SHP-2 is mA modified, and its protein expression is decreased in METTL3-deficient NK cells. Reduced SHP-2 activity renders NK cells hyporesponsive to IL-15, which is associated with suppressed activation of the AKT and MAPK signaling pathway in METTL3-deficient NK cells. These findings show that mA methylation safeguards the homeostasis and tumor immunosurveillance function of NK cells.
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http://dx.doi.org/10.1038/s41467-021-25803-0DOI Listing
September 2021

Mesenchymal stem cells transfected with anti-miRNA-204-3p inhibit acute rejection after heart transplantation by targeting C-X-C motif chemokine receptor 4 (CXCR4) .

J Thorac Dis 2021 Aug;13(8):5077-5092

Department of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, China.

Background: Mesenchymal stem cells (MSCs) are a promising treatment for acute rejection (AR) after heart transplantation (HTx) owing to their immunomodulatory functions by promoting the transformation of macrophages from the M0 to M2 phenotype. However, it is undetermined whether surface expression of C-X-C motif chemokine receptor 4 (CXCR4) by MSCs influences macrophage polarization. In this study, we investigated the effects of MSCs on macrophages caused by CXCR4, and detected the underlying mechanism, which may contribute to improving HTx outcomes.

Methods: The MSCs were extracted from rat bone marrow and identified using flow cytometry. We subsequently observed the effects of CXCR4 and anti-miRNA-204-3p on cell proliferation and migration, and the effects on macrophage polarization. Dual luciferase reporter assay was used to explore whether miRNA-204-3p was an upstream microRNA (miRNA) of CXCR4. A series of rescue experiments were performed to further confirm the inhibitory effect of miRNA-204-3p on CXCR4.

Results: The results showed that CXCR4 could promote the proliferation and migration of MSCs. Furthermore, it facilitated MSC-mediated macrophage transformation from the M0 to M2 phenotype. In addition, miRNA-204-3p inhibited the function of CXCR4 of MSCs.

Conclusions: Regulated by miRNA-204-3p, CXCR4 could inhibit the progression of AR after HTx. This study provides a new insight of the treatment of AR after HTx.
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http://dx.doi.org/10.21037/jtd-21-1293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411131PMC
August 2021

Simulation of near-diffraction- and near-dispersion-free OAM pulses with controllable group velocity by combining multiple frequencies, each carrying a Bessel mode.

Opt Lett 2021 Sep;46(18):4678-4681

Optical pulses carrying orbital angular momentum (OAM) have recently gained interest. In general, it might be beneficial to simultaneously achieve: (i) minimum diffraction, (ii) minimum dispersion, and (iii) controllable group velocity. Here, we explore via simulation the generation of near-diffraction-free and near-dispersion-free OAM pulses with arbitrary group velocities by coherently combining multiple frequencies. Each frequency carries a specific Bessel mode with the same topological charge () but different (spatial frequency) values based on space-time correlations. Moreover, we also find that (i) both positive and negative group velocities could be achieved and continuously controlled from the subluminal to superluminal values and (ii) when the is varied from 0 to 10, the simulated value of the group velocity remains the same. However, as the value increases, the pulse duration becomes longer for a given number of frequency lines.
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http://dx.doi.org/10.1364/OL.434266DOI Listing
September 2021

Requirement of RORα for Maintenance and Anti-Tumor Immunity of Liver-Resident Natural Killer Cells/ILC1s.

Hepatology 2021 Sep 12. Epub 2021 Sep 12.

Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Backgroud And Aims: Liver type 1 innate lymphoid cells (ILC1s), also known as liver-resident natural killer (NK) cells, comprise a high proportion of total hepatic ILCs. However, factors regulating their maintenance and function remain unclear.

Approach And Results: In this study, we found high expression of RORα in liver-resident NK (LrNK) cells/ILC1s. Mice with conditional ablation of Rora in LrNK cells/ILC1s and conventional NK (cNK) cells had decreased LrNK cells/ILC1s but normal numbers of cNK cells. RORα-deficient LrNK cells/ILC1s displayed increased apoptosis and significantly altered transcriptional profile. Using a murine model of colorectal cancer liver metastasis, we found that RORα conditional deficiency resulted in more aggressive liver tumor progression and impaired effector molecule expression in LrNK cells/ILC1s. Consequently, treatment with the RORα agonist efficiently limited liver metastases and promoted effector molecule expression of LrNK cells/ILC1s.

Conclusion: Together, our study unveils a previously undefined role of RORα in LrNK cell/ILC1 maintenance and function, providing insights into the harnessing of LrNK cell/ILC1 activity in the treatment of liver cancer.
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http://dx.doi.org/10.1002/hep.32147DOI Listing
September 2021

Generation of integration-free human iPSC line LCPHi001-A from a Parkinson's disease patient carrying the RecNciI mutation in GBA gene.

Stem Cell Res 2021 Aug 26;56:102514. Epub 2021 Aug 26.

The Institute for Tissue Engineering and Regenerative Medicine, Stem Cell and Regenerative Medicine Laboratory, Liaocheng University/Liaocheng People's Hospital, Liaocheng 252000, Shandong, China; The Innovation Institute for Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China. Electronic address:

Parkinson's disease (PD) is a neurodegenerative disease caused by environmental and genetic factors. The identified PD genes include SNCA, LRRK2, Parkin, DJ-1, PINK1, and ATP13A2. Mutations in the glucocerebrosidase (GBA) gene were reported to be associated with PD in different ethnic populations. Here we generated a novel induced pluripotent stem cell (iPSC) line LCPHi001-A from a PD patient carrying RecNciI mutation (c.1448 T > C, c.1483G > C, and c.1497G > C) in GBA by non-integrative episomal plasmids. The LCPHi001-A line expressed pluripotency markers, displayed differentiation capacity to three germ layers in vivo, and had the normal karyotype.
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http://dx.doi.org/10.1016/j.scr.2021.102514DOI Listing
August 2021

Structural basis of malarial parasite RIFIN-mediated immune escape against LAIR1.

Cell Rep 2021 Aug;36(8):109600

Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Influenza Research and Early Warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

Malaria infection by Plasmodium falciparum continues to pose a global threat to the human population. P. falciparum expresses variable erythrocyte surface antigens such as RIFINs. Public antibodies with LAIR1 insertion have been identified from malarial patients against a subset of RIFINs. In this study, we solve a LAIR1-binding RIFIN structure: the complex structures of two RIFINs bound to mutated or wild-type LAIR1 in two distinct patterns. Notably, the two RIFINs engage similar binding sites on LAIR1 with different angles, and the RIFIN-binding sites overlap with the collagen-binding site. Surprisingly, RIFINs use completely different binding sites to bind to LAIR1 or LILRB1, indicating the kaleidoscopic change of RIFINs. We then verify that RIFIN could induce LAIR1-mediated cell signaling, and LAIR1-containing antibodies could block the pathway. The findings of this study provide structural insights into the mechanism of the immune escape of P. falciparum and the endless arms race between parasite and host.
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http://dx.doi.org/10.1016/j.celrep.2021.109600DOI Listing
August 2021

Metamorphosis in warming oceans: a microbe-larva perspective.

Trends Ecol Evol 2021 Aug 18. Epub 2021 Aug 18.

Kewalo Marine Laboratory, University of Hawai'i at Mānoa, Honolulu, HI 96813, USA. Electronic address:

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http://dx.doi.org/10.1016/j.tree.2021.07.010DOI Listing
August 2021

[n-3 Polyunsaturated fatty acid attenuates hyperhomocysteinemia-induced hepatic steatosis by increasing hepatic LXA content].

Sheng Li Xue Bao 2021 Aug;73(4):551-558

Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin 300070, China.

Nonalcoholic fatty liver disease (NAFLD) and hyperhomocysteinemia (HHcy) both are major health problems worldwide, whose incidence are closely related with each other. We previously reported the mechanism of HHcy-caused hepatic steatosis, but the role of n-3 polyunsaturated fatty acid (n-3 PUFA) in HHcy-induced hepatic steatosis remains unclear. In this study, 6-week-old C57BL/6 male mice were given a high methionine diet (HMD, 2% methionine diet), and plasma homocysteine levels were measured by ELISA to confirm the establishment of an HHcy model. Meantime, mice were fed HMD with or without n-3 PUFA supplement for 8 weeks to determine the role and mechanism of n-3 PUFA in hepatic steatosis induced by HHcy. Results showed that n-3 PUFA significantly improved hepatic lipid deposition induced by HHcy. qRT-PCR analysis demonstrated that n-3 PUFA inhibited the upregulation of Cd36, a key enzyme of fatty acid uptake, caused by HHcy. Further, the inhibition of hepatic Cd36 expression was associated with the inactivation of aryl hydrocarbon receptor (Ahr) induced by n-3 PUFA. Of note, mass spectrometry revealed that hepatic content of lipoxin A (LXA) was significantly increased in HMD+n-3 PUFA-fed mice compared with that in HMD-fed mice. In primary cultured hepatocytes, LXA treatment markedly reversed homocysteine-evoked Cd36 upregulation and Ahr activation, which resulted in reduced lipid accumulation. In conclusion, we demonstrate that n-3 PUFA inactivates HHcy-induced Ahr-Cd36 pathway by increasing hepatic LXA content, which alleviates hepatic steatosis. Thus, our results may provide a potential strategy for treatment of NAFLD.
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August 2021

Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids.

Nat Commun 2021 08 17;12(1):4976. Epub 2021 Aug 17.

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, China.

To construct a superior microbial cell factory for chemical synthesis, a major challenge is to fully exploit cellular potential by identifying and engineering beneficial gene targets in sophisticated metabolic networks. Here, we take advantage of CRISPR interference (CRISPRi) and omics analyses to systematically identify beneficial genes that can be engineered to promote free fatty acids (FFAs) production in Escherichia coli. CRISPRi-mediated genetic perturbation enables the identification of 30 beneficial genes from 108 targets related to FFA metabolism. Then, omics analyses of the FFAs-overproducing strains and a control strain enable the identification of another 26 beneficial genes that are seemingly irrelevant to FFA metabolism. Combinatorial perturbation of four beneficial genes involving cellular stress responses results in a recombinant strain ihfA-aidB-ryfA-gadA, producing 30.0 g L FFAs in fed-batch fermentation, the maximum titer in E. coli reported to date. Our findings are of help in rewiring cellular metabolism and interwoven intracellular processes to facilitate high-titer production of biochemicals.
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http://dx.doi.org/10.1038/s41467-021-25243-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371096PMC
August 2021

LncRNA CTD-3252C9.4 modulates pancreatic cancer cell survival and apoptosis through regulating IFI6 transcription.

Cancer Cell Int 2021 Aug 16;21(1):433. Epub 2021 Aug 16.

State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjiaxiang Avenue, Nanjing, Jiangsu, China.

Background: Pancreatic cancer (PC) is one of the most lethal cancer types with high degree of malignancy and poor prognosis. Recent studies have shown that long non-coding RNAs (lncRNAs) were associated with the initiation and progression of pancreatic cancer. In the current study, we have investigated the expression, biological function and mechanism of a lncRNA CTD-3252C9.4 in pancreatic cancer.

Methods: The expression of CTD-3252C9.4 in pancreatic cancer cells and tissues was measured by qRT-PCR. In vitro and in vivo functional experiments assays were implemented for identifying CTD-3252C9.4 function in pancreatic cancer. Molecular relationships among CTD-3252C9.4, IRF1 and IFI6 were investigated via luciferase reporter assay, pulldown assay and ChIP assays.

Results: CTD-3252C9.4 was found remarkably decreased in pancreatic cancer cells and tissues. Overexpression of CTD-3252C9.4 suppressed migration, invasion and proliferation, yet facilitated apoptosis of pancreatic cancer cells both in vitro and in vivo. Then, IFI6 was identified as a downstream target that could be down-regulated by CTD-3252C9.4 and IFI6 overexpression could counteract the effects of CTD-3252C9.4 upregulation on the survival and apoptosis of pancreatic cancer cells. Furthermore, mechanism experiments revealed that IRF1 was a transcriptional factor of IFI6 that can be blocked by CTD-3252C9.4 to inhibit IFI6 transcription.

Conclusion: Our data indicated that CTD-3252C9.4 could promote pancreatic cancer cell apoptosis and restrain cell growth via binding IRF1 and preventing the transcription of IFI6, which may become a potential therapeutic target for pancreatic cancer.
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http://dx.doi.org/10.1186/s12935-021-02142-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365976PMC
August 2021

Photocatalyst-enzyme hybrid systems for light-driven biotransformation.

Biotechnol Adv 2021 Jul 27:107808. Epub 2021 Jul 27.

Frontier Science Centre for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), Collaborative Innovation Centre of Chemical Science and Engineering (Tianjin), and School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China. Electronic address:

Enzymes catalyse target reactions under mild conditions with high efficiency, as well as excellent regional-, stereo-, and enantiomeric selectivity. Photocatalysis utilises sustainable and environment-friendly light power to realise efficient chemical conversion. By combining the interdisciplinary advantages of photo- and enzymatic catalysis, the photocatalyst-enzyme hybrid systems have proceeded various light-driven biotransformation with high efficiency under environmentally benign conditions, thus, attracting unparalleled focus during the last decades. It has also been regarded as a promising pathway towards green chemistry utilising ubiquitous solar energy. This systematic review gives insight into this research field by classifying the existing photocatalyst-enzyme hybrid systems into three sections based on different hybridizing modes between photo- and enzymatic catalysis. Furthermore, existing challenges and proposed strategies are discussed within this context. The first system summarised is the cofactor-mediated hybrid system, in which natural/artificial cofactors act as reducing equivalents that connect photocatalysts with enzymes for light-driven enzymatic biotransformation. Second, the direct contact-based photocatalyst-enzyme hybrid systems are described, including two different kinds of electron exchange sites on the enzyme molecules. Third, some cases where photocatalysts and enzymes are integrated into a reaction cascade with specific intermediates will be discussed in the following chapter. Finally, we provide perspective concerning the future of this field.
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http://dx.doi.org/10.1016/j.biotechadv.2021.107808DOI Listing
July 2021

Ferroptosis-Strengthened Metabolic and Inflammatory Regulation of Tumor-Associated Macrophages Provokes Potent Tumoricidal Activities.

Nano Lett 2021 08 22;21(15):6471-6479. Epub 2021 Jul 22.

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, People's Republic of China.

Modulation of tumor-associated macrophages (TAMs) holds promise for cancer treatment, mainly relying on M1 signaling activation and pro-inflammatory promotion. Nevertheless, the antitumor activity is often limited by the anti-inflammatory factors in the tumor microenvironment. Moreover, the metabolic function of TAMs is also critical to tumor progression. However, there are a few strategies that can simultaneously regulate both inflammatory and metabolic functions to achieve safe and potent antitumor activation of TAMs. Herein, we demonstrate that an iron-based metal organic framework nanoparticle and a ferroptosis-inducing agent synergistically induce mitochondrial alternation in TAMs, resulting in a radical metabolic switch from mitochondrial oxidative phosphorylation to glycolysis, which is resistant to anti-inflammatory stimuli challenge. The ferroptosis stress strengthened by the nanoformulation also drives multiple pro-inflammatory signaling pathways, enabling macrophage activation with potent tumoricidal activities. The ferroptosis-strengthened macrophage regulation strategy present in this study paves the way for TAM-centered antitumoral treatment to overcome the limitations of conventional methods.
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http://dx.doi.org/10.1021/acs.nanolett.1c01401DOI Listing
August 2021

Enhanced nitrogen and phosphorus removal by natural pyrite-based constructed wetland with intermittent aeration.

Environ Sci Pollut Res Int 2021 Jul 20. Epub 2021 Jul 20.

Anhui Provincial Engineering Laboratory for Rural Water Environment and Resources, School of Civil and Hydraulic Engineering, Hefei University of Technology, Hefei, 230009, China.

Four subsurface flow constructed wetlands (SFCWs) filled with different substrates including ceramsite, ceramsite+pyrite, ceramsite+ferrous sulfide, and ceramsite+pyrite+ferrous sulfide (labeled as SFCW-S1, SFCW-S2, SFCW-S3, and SFCW-S4) were constructed, and the removal of nitrogen and phosphorus by these SFCWs coupled with intermittent aeration in the front section was discussed. The key findings from different substrate analyses, including nitrification and denitrification rate, enzyme activity, microbial community structure, and the X-ray diffraction, revealed the nitrogen and phosphorus removal mechanism. The results showed that the nitrogen and phosphorus removal efficiency for SFCW-S1 always remained the lowest, and the phosphorus removal efficiency for SFCW-S4 was recorded as the highest one. However, after controlling the dissolved oxygen by intermittent aeration in the front section of SFCWs, the nitrogen and phosphorus removal efficiencies of SFCWs-S2 and S4 became higher than those of SFCW-S1, and SFCW-S3. It was noticed that the pollutants were removed mainly in the front section of the SFCWs. Both precipitation and adsorption on the substrate were the main mechanisms for phosphorus removal. A minute difference of nitrification rate and ammonia monooxygenase activity was observed in the SFCWs' aeration zone. The denitrification rates, nitrate reductase, nitrite reductase, and electron transport system activity for SFCW-S2 and SFCW-S4 were higher than those detected for SFCW-S1 and SFCW-S3 in the non-aerated zone. Proteobacteria was the largest phyla found in the SFCWs. Moreover, Thiobacillus occupied a large proportion found in SFCW-S2, and SFCW-S4, and it played a crucial role in pyrite-driven autotrophic denitrification.
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http://dx.doi.org/10.1007/s11356-021-15461-6DOI Listing
July 2021

Enhanced biohydrogen production from corn straw by basalt fiber addition.

Bioresour Technol 2021 Oct 13;338:125528. Epub 2021 Jul 13.

Key Laboratory of New Materials and Facilities for Rural Renewable Energy of Ministry of Agriculture and Rural Affairs, College of Mechanical & Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Henan International Joint Laboratory of Biomass Energy and Nanomaterials, Henan Agricultural University, Zhengzhou 450002, China; Henan Collaborative Innovation Center of Biomass Energy, Henan Agricultural University, Zhengzhou 450002, China. Electronic address:

The aim of this work was to study the impact of basalt fiber (BF) on hydrogen fermentation of corn straw. The maximum of hydrogen yield and corn straw conversion rate respectively achieved 323.94 mL and 5.23% by adding 1.5 g/L BF particle with the size of 300-400 mesh, which increased by 15.74% and 15.6% respectively than control group. The BF could promote the growth of photosynthetic bacteria, subsequently influencing the products distribution and hydrogen generation. Overall, this investigation demonstrated that BF addition is an effective way to enhance biohydrogen production from corn straw.
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http://dx.doi.org/10.1016/j.biortech.2021.125528DOI Listing
October 2021

Tunable Doppler shift using a time-varying epsilon-near-zero thin film near 1550  nm.

Opt Lett 2021 Jul;46(14):3444-3447

We experimentally investigate the tunable Doppler shift in an 80 nm thick indium-tin-oxide (ITO) film at its epsilon-near-zero (ENZ) region. Under strong and pulsed excitation, ITO exhibits a time-varying change in the refractive index. A maximum frequency redshift of 1.8 THz is observed in the reflected light when the pump light has a peak intensity of ∼140/ and a pulse duration of ∼580, at an incident angle of 40°. The frequency shift increases with the increase in pump intensity and saturates at the intensity of ∼140/. When the pump pulse duration increases from ∼580 to ∼1380, the maximum attainable frequency shift decreases from 1.8 THz to 0.7 THz. In addition, the pump energy required to saturate the frequency shift decreases with the increase in pump pulse duration for ∼<1 and remains unchanged for ∼>1 durations. Tunability exists among the pump pulse energy, duration, and incident angle for the Doppler shift of the ITO-ENZ material, which can be employed to design efficient frequency shifters for telecom applications.
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http://dx.doi.org/10.1364/OL.430106DOI Listing
July 2021

Adiabatic Frequency Conversion Using a Time-Varying Epsilon-Near-Zero Metasurface.

Nano Lett 2021 07 12;21(14):5907-5913. Epub 2021 Jul 12.

Department of Electrical and Computer Engineering, University of Southern California, Los Angeles, California 90089, United States.

A time-dependent change in the refractive index of a material leads to a change in the frequency of an optical beam passing through that medium. Here, we experimentally demonstrate that this effect-known as adiabatic frequency conversion (AFC)-can be significantly enhanced by a nonlinear epsilon-near-zero-based (ENZ-based) plasmonic metasurface. Specifically, by using a 63-nm-thick metasurface, we demonstrate a large, tunable, and broadband frequency shift of up to ∼11.2 THz with a pump intensity of 4 GW/cm. Our results represent a decrease of ∼10 times in device thickness and 120 times in pump peak intensity compared with the cases of bare, thicker ENZ materials for the similar amount of frequency shift. Our findings might potentially provide insights for designing efficient time-varying metasurfaces for the manipulation of ultrafast pulses.
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http://dx.doi.org/10.1021/acs.nanolett.1c00550DOI Listing
July 2021

Nautilus pompilius.

Trends Genet 2021 Jul 1. Epub 2021 Jul 1.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China; CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; University of the Chinese Academy of Sciences, Beijing, 100049, China; Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao 266071, China. Electronic address:

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http://dx.doi.org/10.1016/j.tig.2021.06.011DOI Listing
July 2021

Research Progress and Development Trends of Acoustic Metamaterials.

Molecules 2021 Jun 30;26(13). Epub 2021 Jun 30.

School of Navel Architecture and Ocean Engineering, Jiangsu University of Science and Technology, Zhenjiang 212000, China.

Acoustic metamaterials are materials with artificially designed structures, which have characteristics that surpass the behavior of natural materials, such as negative refraction, anomalous Doppler effect, plane focusing, etc. This article mainly introduces and summarizes the related research progress of acoustic metamaterials in the past two decades, focusing on meta-atomic acoustic metamaterials, metamolecular acoustic metamaterials, meta-atomic clusters and metamolecule cluster acoustic metamaterials. Finally, the research overview and development trend of acoustic metasurfaces are briefly introduced.
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http://dx.doi.org/10.3390/molecules26134018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271960PMC
June 2021

RNA-Seq analysis and WGCNA reveal dynamic molecular responses to air exposure in the hard clam Mercenaria mercenaria.

Genomics 2021 Jul 18;113(4):2847-2859. Epub 2021 Jun 18.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao 266071, China. Electronic address:

Intertidal bivalves are constantly exposed to air due to daily and seasonal tidal cycles. The hard clam Mercenaria mercenaria is an economically important bivalve species and often subjected to air exposure for more than 10 days during long-distance transportation. Hard clam exhibits remarkable tolerance to air exposure. In this study, we performed RNA sequencing on hemocytes of M. mercenaria exposed to air for 0, 1, 5, 10, 20 and 30 days. The overall and dynamic molecular responses of hard clams to air exposure were revealed by different transcriptomic analysis strategies. As a result, most cytochrome P450 1A and 3A, and monocarboxylate transporter family members were up-regulated during air exposure. Additionally, the dominant molecular process in response to 5-d, 10-d, 20-d and 30-d air exposure was refolding of misfolded proteins in endoplasmic reticulum, lysosome-mediated degradation of phospholipids, protein metabolism and reorganization of cytoskeleton, and activation of anti-apoptotic process, respectively. Our results facilitated comprehensive understanding of the tolerance mechanisms of intertidal bivalves to air exposure.
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http://dx.doi.org/10.1016/j.ygeno.2021.06.025DOI Listing
July 2021

Observation of Negative Terahertz Photoconductivity in Large Area Type-II Dirac Semimetal PtTe_{2}.

Phys Rev Lett 2021 Jun;126(22):227402

Department of Physics, Shanghai University, Shanghai 200444, China.

As a newly emergent type-II Dirac semimetal, platinum telluride (PtTe_{2}) stands out from other two dimensional noble-transition-metal dichalcogenides for the unique band structure and novel physical properties, and has been studied extensively. However, the ultrafast response of low energy quasiparticle excitation in terahertz frequency remains nearly unexplored yet. Herein, we employ optical pump-terahertz probe (OPTP) spectroscopy to systematically study the photocarrier dynamics of PtTe_{2} thin films with varying pump fluence, temperature, and film thickness. Upon photoexcitation the terahertz photoconductivity (PC) of PtTe_{2} films shows abrupt increase initially, while the terahertz PC changes into negative value in a subpicosecond timescale, followed by a prolonged recovery process that lasted a few nanoseconds. The magnitude of both positive and negative terahertz PC response shows strongly pump fluence dependence. We assign the unusual negative terahertz PC to the formation of small polaron due to the strong electron-phonon (e-ph) coupling, which is further substantiated by temperature and film thickness dependent measurements. Moreover, our investigations give a subpicosecond timescale of simultaneous carrier cooling and polaron formation. The present study provides deep insights into the underlying dynamics evolution mechanisms of photocarrier in type-II Dirac semimetal upon photoexcitation, which is of crucial importance for designing PtTe_{2}-based optoelectronic devices.
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http://dx.doi.org/10.1103/PhysRevLett.126.227402DOI Listing
June 2021

Multistep Dissociation of Fluorine Molecules under Extreme Compression.

Phys Rev Lett 2021 Jun;126(22):225704

Department of Chemistry and Biochemistry, California State University, Northridge, California 91220, USA.

All elements that form diatomic molecules, such as H_{2}, N_{2}, O_{2}, Cl_{2}, Br_{2}, and I_{2}, are destined to become atomic solids under sufficiently high pressure. However, as revealed by many experimental and theoretical studies, these elements show very different propensity and transition paths due to the balance of reduced volume, lone pair electrons, and interatomic bonds. The study of F under pressure can illuminate this intricate behavior since F, owing to its unique position on the periodic table, can be compared with H, with N and O, and also with other halogens. Nevertheless, F remains the only element whose solid structure evolution under pressure has not been thoroughly studied. Using a large-scale crystal structure search method based on first principles calculations, we find that, before reaching an atomic phase, F solid transforms first into a structure consisting of F_{2} molecules and F polymer chains and then into a structure consisting of F polymer chains and F atoms, a distinctive evolution with pressure that has not been seen in any other elements. Both intermediate structures are found to be metallic and become superconducting, a result that adds F to the elemental superconductors.
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http://dx.doi.org/10.1103/PhysRevLett.126.225704DOI Listing
June 2021

Identification of novel bat coronaviruses sheds light on the evolutionary origins of SARS-CoV-2 and related viruses.

Cell 2021 08 9;184(17):4380-4391.e14. Epub 2021 Jun 9.

Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian 271000, China; School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian 271000, China. Electronic address:

Despite the discovery of animal coronaviruses related to SARS-CoV-2, the evolutionary origins of this virus are elusive. We describe a meta-transcriptomic study of 411 bat samples collected from a small geographical region in Yunnan province, China, between May 2019 and November 2020. We identified 24 full-length coronavirus genomes, including four novel SARS-CoV-2-related and three SARS-CoV-related viruses. Rhinolophus pusillus virus RpYN06 was the closest relative of SARS-CoV-2 in most of the genome, although it possessed a more divergent spike gene. The other three SARS-CoV-2-related coronaviruses carried a genetically distinct spike gene that could weakly bind to the hACE2 receptor in vitro. Ecological modeling predicted the co-existence of up to 23 Rhinolophus bat species, with the largest contiguous hotspots extending from South Laos and Vietnam to southern China. Our study highlights the remarkable diversity of bat coronaviruses at the local scale, including close relatives of both SARS-CoV-2 and SARS-CoV.
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http://dx.doi.org/10.1016/j.cell.2021.06.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188299PMC
August 2021

A multicenter randomized phase II trial of hyperthermia combined with TPF induction chemotherapy compared with TPF induction chemotherapy in locally advanced resectable oral squamous cell carcinoma.

Int J Hyperthermia 2021 ;38(1):939-947

Department of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Hyperthermia has been reported to cause cancer stage regression, thus providing surgical opportunities in patients with unresectable tumors and improving the quality of life of patients by preserving certain organs.

Methods: A prospective open-label phase II trial was conducted to evaluate the efficacy of hyperthermia combined with induction chemotherapy in patients with locally advanced resectable oral squamous cell carcinoma (OSCC). Patients received hyperthermia combined with two cycles of 5-fluorouracil, cisplatin, and docetaxel (TPF) induction chemotherapy regimens or TPF induction chemotherapy alone, followed by radical surgery with postoperative radiotherapy. The primary endpoint was the clinical response rate of the induction chemotherapy. The secondary endpoints were overall survival (OS), disease-free survival (DFS), and toxicity.

Results: A total of 120 patients were enrolled, and 115 patients were included in the clinical response analysis. The clinical response rate was significantly higher in the experimental arm than in the control arm (65.45% vs. 40.00%,  = 0.0088). There were no unexpected toxicities, and hyperthermia and induction chemotherapy did not increase the perioperative morbidity rate. Moreover, there was a significant improvement in DFS, but no significant difference in OS between the two arms. In the subgroup analysis, increased OS and DFS rates were associated with patients with favorable clinical response after induction chemotherapy in the total population, experimental arm, and control arm.

Conclusions: Our study demonstrates that hyperthermia combined with induction chemotherapy is associated with a high response rate and provides a new treatment option for patients with resectable stage III or IVA OSCC.
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http://dx.doi.org/10.1080/02656736.2021.1937714DOI Listing
July 2021

Post translational modification-assisted cancer immunotherapy for effective breast cancer treatment.

Chem Sci 2020 Sep 10;11(38):10421-10430. Epub 2020 Sep 10.

Australian Institute for Bioengineering and Nanotechnology, The University of Queensland QLD 4072 Australia

Post translational modifications (PTM) such as phosphorylation are often correlated with tumorigenesis and malignancy in breast cancer. Herein, we report a PTM-assisted strategy as a simplified version of a personalized cancer vaccine for enhanced cancer immunotherapy. Titanium modified dendritic mesoporous silica nanoparticles (TiDMSN) are applied to assist the specific enrichment of phosphorylated tumor antigens released upon immunogenic cell death. This strategy significantly improved the tumor inhibition efficacy in a bilateral breast cancer model and the expansion of both CD8 and CD4 T cells in the distant tumor site. The nanotechnology based PTM-assisted strategy provides a simple and generalizable methodology for effective personalized cancer immunotherapy.
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http://dx.doi.org/10.1039/d0sc02803gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162284PMC
September 2020

LncRNA LINC00473 is involved in the progression of invasive pituitary adenoma by upregulating KMT5A via ceRNA-mediated miR-502-3p evasion.

Cell Death Dis 2021 06 5;12(6):580. Epub 2021 Jun 5.

Department of Neurosurgery, 1st Affiliated Hospital of Kunming Medical University, Kunming, China.

Long noncoding RNAs (lncRNAs) and their crosstalks with other RNAs have been revealed to be closely related to tumorigenesis and development, but their role in invasive pituitary adenoma (IPA) remains largely unclear. In our study, LINC00473 was identified as the most upregulated lncRNA in IPA by whole transcriptome RNA sequencing (RNA-Seq). Further, its related signaling pathway LINC00473/miR-502-3p/KMT5A was obtained by constructing a competing endogenous RNA (ceRNA) regulatory network. Their expression in IPA and non-invasive pituitary adenoma (NIPA) tissues was verified by qRT-PCR. Then the effects and mechanisms of LINC00473 and its ceRNA network on the proliferation of pituitary adenoma (PA) cells were confirmed by gene overexpression or silencing techniques combined with CCK-8 assay, EdU staining, flow cytometry assay, and double luciferase reporter gene assay in PA cell lines AtT-20 and GT1-1 in vitro and in a xenograft model in vivo. LINC00473 is overexpressed in IPA and can promote PA cells proliferation. Mechanistically, overexpression of LINC00473 restricts miR-502-3p through the ceRNA mechanism, upregulates KMT5A expression, and promotes the expression of cyclin D1 and CDK2, which is conducive to the cell cycle process, thereby promoting the proliferation of PA cells, involving IPA progression.
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http://dx.doi.org/10.1038/s41419-021-03861-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179925PMC
June 2021

Highly efficient selective extraction of Mo with novel hydrophobic deep eutectic solvents.

J Air Waste Manag Assoc 2021 Jun 1. Epub 2021 Jun 1.

Key Laboratory of Clean Energy Utilization, State Environmental Protection Center for Coal-Fired Air Pollution Control, Zhejiang University, Hangzhou 310027, Zhejiang, China.

Recycling of valuable metals from spent catalysts in a green way is gaining extensive interest for economic and environment reasons. In this study, we developed novel hydrophobic deep eutectic solvents to extract Mo from spent catalysts. The hydrophobic DESs have been designed and synthesized by mixing one molar of the quaternary ammonium salt and two molars of various saturated fatty acids with different carbon chain lengths. The extraction ability and extraction mechanism of these DESs were studied, some factors influencing the extraction efficiency, including the structure of hydrogen bond acceptors and hydrogen bond donors, initial aqueous pH, reaction time and temperature, phase ratios were investigated. It is found that the synthesized hydrophobic DESs exhibit excellent extraction performance towards Mo, where the Mo distribution ratio is more than 2200 in the presence of other metals, corresponding to extraction efficiency of 99% at optimal reaction conditions. This work reveals a distinct class of materials, guiding an effective and green way for spent catalyst treatment. ImplicationsNovel hydrophobic deep eutectic solvents have been developed to extract Mo from spent catalysts, the synthesized hydrophobic DESs possess several advantages such as green, low price, low toxicity, biodegradable. It exhibits excellent extraction performance under an optimized extraction condition. This work reveals a distinct class of materials, guiding a promising way for green and economical utilization of spent catalysts.
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http://dx.doi.org/10.1080/10962247.2021.1937379DOI Listing
June 2021

A luminescent view of the clickable assembly of LnF nanoclusters.

Nat Commun 2021 05 19;12(1):2948. Epub 2021 May 19.

Institute of Advanced Materials (IAM), Nanjing Tech University, 30 South Puzhu Road, Nanjing, China.

Nanoclusters (NCs) bridge the gap between atoms and nanomaterials in not only dimension but also physicochemical properties. Precise chemical and structural control, as well as clear understanding of formation mechanisms, have been important to fabricate NCs with high performance in optoelectronics, catalysis, nanoalloys, and energy conversion and harvesting. Herein, taking advantage of the close chemical properties of Ln (Ln = Eu, Nd, Sm, Gd, etc.) and Gd-Eu energy transfer ion-pair, we report a clickable LnF nanoparticle assembly strategy allowing reliable fabrication of diversely structured NCs, including single-component, dimeric, core-shelled/core-shell-shelled, and reversely core-shelled/core-shell-shelled, particularly with synergized optical functionalities. Moreover, the purposely-embedded dual luminescent probes offer great superiority for in situ and precise tracking of tiny structural variations and energy transfer pathways within complex nanoarchitectures.
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http://dx.doi.org/10.1038/s41467-021-23176-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136472PMC
May 2021

Management of Vasoproliferative Tumors of the Retina with Macular Complications by Pars Plana Vitrectomy Combined with Episcleral Cryotherapy.

J Ophthalmol 2021 13;2021:6667755. Epub 2021 Apr 13.

Henan Eye Institute, Henan Eye Hospital, Henan Provincial People's Hospital and Henan University People's Hospital, Zhengzhou 450003, Henan, China.

Objective: To evaluate the efficacy of pars plana vitrectomy (PPV) combined with episcleral cryotherapy in treating vasoproliferative tumors of the retina (VPTR) with macular complications.

Methods: In this retrospective noncomparative interventional case-series analysis, we included 11 eyes of ten patients diagnosed with VPTR. All patients underwent comprehensive ophthalmic examinations and were treated with PPV combined with episcleral cryotherapy. Best-corrected visual acuity (BCVA), tumor activity, retinal morphological structure, and postoperative complications were evaluated.

Results: Macular complications included epimacular membrane ( = 10), macular hole ( = 3), and macular edema ( = 1). Tumors were treated with triple freeze-thaw episcleral cryotherapy during PPV. The mean logarithm of minimal angle of resolution (logMAR) BCVA dropped from 0.62 ± 0.58 to 0.39 ± 0.46. The difference between the mean values of logMAR BCVA before and after treatment was statistically significant ( = 2.48, =0.033). The tumor activity was controlled effectively in nine cases. Compared with preoperative tumor activity, tumor activity after treatment was significantly lower ( < 0.01). The increase of central retinal thickness and the disruption of retinal layers were associated with macular holes, macular edema, and retinal proliferative membrane. After the treatment, visual acuity improved in 91% of the cases, and 73% had no long-term complications.

Conclusion: PPV combined with episcleral cryotherapy promoted tumor regression, preserved retinal integrity, and improved visual acuity. Thus, the combination of PPV with episcleral cryotherapy can be considered effective and safe for the management of VPTR with macular complications.
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http://dx.doi.org/10.1155/2021/6667755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057872PMC
April 2021

Rational Design of Dendritic Mesoporous Silica Nanoparticles' Surface Chemistry for Quantum Dot Enrichment and an Ultrasensitive Lateral Flow Immunoassay.

ACS Appl Mater Interfaces 2021 May 3;13(18):21507-21515. Epub 2021 May 3.

Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland 4072, Australia.

Lateral flow immunoassays (LFIAs) have drawn much attention in point-of-care diagnostic applications, and the development of high-performance label materials is the key. In this study, the impact of the surface chemistry of dendritic mesoporous silica nanoparticles (DMSNs) on their enrichment performance toward quantum dots (QDs) and signal amplification of the resultant DMSNs-QDs as label materials have been investigated. A series of DMSNs with controllable amino/thiol group densities have been synthesized. It is demonstrated that the amino groups are beneficial for QD fluorescence preservation, owing to the amino-based surface passivation, while the thiol groups are responsible for increasing the loading capacity of QDs due to the thiol-metal coordination. The optimized DMSNs-QDs labels with an amino density of 153 μmol g and a thiol density of 218 μmol g displayed sufficient QD fluorescence preservation (89.4%) and high QD loading capacity (1.55 g g). Ultrasensitive detection of serum amyloid A (SAA) with a detection limit of 10 pg mL with the naked eye was achieved, which is 1 order of magnitude higher than that reported in the literature. This study provides insights into the development of advanced label materials and an ultrasensitive LFIA for future bioassay applications.
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http://dx.doi.org/10.1021/acsami.1c02149DOI Listing
May 2021

Genotyping and Molecular Characterization of Classical Swine Fever Virus Isolated in China during 2016-2018.

Viruses 2021 04 12;13(4). Epub 2021 Apr 12.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150069, China.

Classical swine fever (CSF) is a highly contagious disease of swine caused by classical swine fever virus (CSFV). For decades the disease has been controlled in China by a modified live vaccine (C-strain) of genotype 1. The emergent genotype 2 strains have become predominant in China in the past years that are genetically distant from the vaccine strain. Here, we aimed to evaluate the current infectious status of CSF, and for this purpose 24 isolates of CSFV were identified from different areas of China during 2016-2018. Phylogenetic analysis of NS5B, E2 and full genome revealed that the new isolates were clustered into subgenotype 2.1d and 2.1b, while subgenotype 2.1d was predominant. Moreover, E2 and E displayed multiple variations in neutralizing epitope regions. Furthermore, the new isolates exhibited capacity to escape C-strain-derived antibody neutralization compared with the Shimen strain (genotype 1). Potential positive selection sites were identified in antigenic regions of E2 and E, which are related with antibody binding affinity. Recombination events were predicted in the new isolates with vaccine strains in the E2 gene region. In conclusion, the new isolates showed molecular variations and antigenic alterations, which provide evidence for the emergence of vaccine-escaping mutants and emphasize the need of updated strategies for CSF control.
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http://dx.doi.org/10.3390/v13040664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069065PMC
April 2021
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