Publications by authors named "Hao Qing Zhang"

5 Publications

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Balanced fertilization over four decades has sustained soil microbial communities and improved soil fertility and rice productivity in red paddy soil.

Sci Total Environ 2021 Nov 24;793:148664. Epub 2021 Jun 24.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China.

The influence of long-term fertilization on soil microbial communities is critical for revealing the association between belowground microbial flora and aboveground crop productivity-a relationship of great importance to food security, environmental protection, and ecosystem functions. Here, we examined shifts in soil chemical properties, microbial communities, and the nutrient uptake and yield of rice subjected to different chemical and organic fertilization treatments over a 40-year period in red paddy soil. Ten different treatments were used: a control without fertilizer, and applications of nitrogen (N), phosphorus (P), potassium (K), NP, NK, PK, NPK, double NPK, or NPK plus manure. Compared with the effects of withholding one or two nutrients (N, P, or K), the balanced application of chemical NPK and organic fertilizers markedly improved soil nutrient status and rice yield. This improvement of soil fertility and rice yield was not associated with bacterial, archaeal, or fungal alpha diversities. The bacterial abundance and community structure and archaeal abundance effectively explained the variation in rice yield, whereas those of fungi did not. The community structure of bacteria and archaea, but not that of fungi, was correlated with soil properties. Among various soil properties, P was the key factor influencing rice yield and soil microbial communities because of the extremely low content of soil available P. Seven keystones at the operational taxonomic unit level were identified: four archaea (belonging to Thermoplasmata, Methanosaeta, Bathyarchaeia, and Nitrososphaeraceae) and three bacteria (in Desulfobacteraceae and Acidobacteriales). These keystones, which were mainly related to soil C and N transformation and pH, may work cooperatively to influence rice yield by regulating soil fertility. Our results collectively suggest that four decades of balanced fertilization has sustained the bacterial and archaeal abundances, bacterial community structure, and keystones, which potentially contribute to soil fertility and rice yield in red paddy soil.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148664DOI Listing
November 2021

[Application of Next-Generation Sequencing in Screening of Thalassemia Gene in 11212 Pregnant Women in Suxian and Beihu Districts of Chenzhou City, Hunan Province].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Feb;29(1):188-192

Center of Prenatal Diagnosis, Chenzhou No.1 People's Hospital, Chenzhou 423000,Hunan Province, China,E-mail:

Objective: To understand the carrying rate, gene mutation frequency and composition ratio of thalassemia in pregnant women in Suxian and Beihu districts of Chenzhou, Hunan Province.

Methods: Thalassemia gene in 11 212 samples was analyzed by using Next-Generation Sequencing.

Results: Among the 11 212 samples, 938 were diagnosed as thalassemia, in which 618 (5.51%) were diagnosed as α-thalassemia, 268 (2.39%) as β-thalassemia, 29(0.26%)as abnormal hemoglobin and 23 (0.21%) as αβ-thalassemia. The gene mutations of --SEA /αα(40.29%) and -α3.7/αα(37.7%) in α-thalassemia were the most common, while for β- thalassemia, the most commonly gene mutation were β41-42M/βN(24.26%) and β654M/βN(23.88%). The detection rate of rare type α,β-thalassemia gene was 0.19%(21/11 212), 0.53%(59/11 212), respectively.

Conclusion: The carrying rate of thalassemia in pregnant women is 8.37% in Suxian and Beihu districts of Chenzhou city, and the genotypes are complex. Next-Generation Sequencing can detect rare thalassemia genes and new gene mutations effectively.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.01.029DOI Listing
February 2021

Improved Root Growth by Liming Aluminum-Sensitive Rice Cultivar or Cultivating an Aluminum-Tolerant One Does Not Enhance Fertilizer Nitrogen Recovery Efficiency in an Acid Paddy Soil.

Plants (Basel) 2020 Jun 19;9(6). Epub 2020 Jun 19.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.

The root is the main site of nitrogen (N) acquisition and aluminum (Al) toxicity. The objective of this study is to investigate whether liming and cultivation of an Al-tolerant rice ( L.) cultivar can improve root growth, thereby increasing N acquisition by rice plants in acid paddy soil. Two rice cultivars ('B690', Al-sensitive, and 'Yugeng5', Al-tolerant) were cultivated with N-labeled urea, and with or without lime in an acid paddy soil (pH 4.9) in pots. We examined root and shoot growth, soil pH, soil exchangeable Al, N uptake, N distribution in plant-soil system, and fertilizer N recovery efficiency. Results showed that liming improved the root growth of 'B690' by decreasing soil exchangeable Al concentrations, in both N-limited and N-fertilized soils. Liming enhanced the N uptake of 'B690' only in the absence of N fertilizer. The root weight of 'Yugeng5' was greater than that of 'B690' without lime, but the two cultivars showed similar N uptake. The fertilizer N recovery efficiency and N loss did not differ significantly between limed and non-limed conditions, or between the two rice cultivars. Thus, liming an Al-sensitive rice cultivar and cultivating an Al-tolerant one improves root growth, but does not enhance fertilizer N recovery efficiency in the present acid paddy soil.
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http://dx.doi.org/10.3390/plants9060765DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355884PMC
June 2020

[Effects of combined application of chemical fertilizer and organic manure on wheat yield and leaching of residual nitrate-N in dryland soil].

Ying Yong Sheng Tai Xue Bao 2018 Apr;29(4):1240-1248

College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, Shaanxi, China.

The effects of optimum nitrogen (N) fertilization rate with and without adding manure on wheat yield and leaching of residual nitrate-N in soil profile were examined in Weibei dryland, Shaanxi with a field experiment combined different N fertilization rates (0, 75, 150, 225, 300 kg N·hm) and organic manure (0 and 30 t·hm). The results showed that, compared to chemical N fertilizer, combined application of inorganic fertilizer and organic manure increased winter wheat yield by 14.7% when N fertilization rate was reduced by 27.1%. The highest yield was obtained when 150 kg·hm of N rate was combined with the manure (N+M). The combination of N fertilizer and manure promoted N uptake of wheat grain and increased N use efficiency by 20.2%. The highest N use efficiency was recorded in the N+M treatment. In addition, the lea-ching of residual nitrate-N during the wheat growing season and the leaching of nitrate-N during summer fallow were decreased. When N application rate was lower than 115 kg·hm, N fertilizer combined with organic manure reduced the amount of nitrate-N leaching in summer fallow. We recommend the combined application of organic manure with about 150 kg·hm of N fertilizers in Weibei dryland to guarantee high winter wheat yield, N use efficiency, and reduce excessive residue of fertilizer N in the soil.
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http://dx.doi.org/10.13287/j.1001-9332.201804.023DOI Listing
April 2018

Berberine inhibits cytosolic phospholipase A2 and protects against LPS-induced lung injury and lethality independent of the alpha2-adrenergic receptor in mice.

Shock 2008 May;29(5):617-22

Department of Pathophysiology, Medical College of Jinan University, Guangzhou, Guangdong, China.

Acute lung injury is still a significant clinical problem having a high mortality rate despite significant advances in antimicrobial therapy and supportive care made in the past few years. Our previous study demonstrated that berberine (Ber) remarkably decreased mortality and attenuated the lung injury in mice challenged with LPS, but the mechanism behind this remains unclear. Here, we report that pretreatment with Ber significantly reduced pulmonary edema, neutrophil infiltration, and histopathological alterations; inhibited protein expression and phosphorylation of cytosolic phospholipase A2; and decreased thromboxane A2 release induced by LPS. Yohimbine, an alpha2-adrenergic receptor antagonist, did not antagonize these actions of Ber. Furthermore, pretreatment with Ber decreased TNF-alpha production and mortality in mice challenged with LPS, which were enhanced by yohimbine, and Ber combined with yohimbine also improved survival rate in mice subjected to cecal ligation and puncture. Taken together, these observations indicate that Ber attenuates LPS-induced lung injury by inhibiting TNF-alpha production and cytosolic phospholipase A2 expression and activation in an alpha2-adrenoceptor-independent manner. Berberine combined with yohimbine might provide an effective therapeutic approach to acute lung injury during sepsis.
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http://dx.doi.org/10.1097/SHK.0b013e318157ea14DOI Listing
May 2008
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