Publications by authors named "Hao Luo"

545 Publications

Dynamics of Hepatitis B Virus Pregenomic RNA in Chronic Hepatitis B Patients With Antiviral Therapy Over 9 Years.

Front Med (Lausanne) 2022 29;9:851717. Epub 2022 Apr 29.

Department of Infectious Diseases, Peking University First Hospital, Beijing, China.

Serum hepatitis B virus pregenomic RNA (HBV pgRNA) is a potential biomarker that is correlated with covalently closed circular DNA. The long-term dynamics of HBV pgRNA in patients with chronic hepatitis B need to be explored. One hundred naïve nucleos(t)ide analog-treated patients with chronic hepatitis B were enrolled to analyze the dynamics of HBV pgRNA over 9 years. The positive rates of HBV pgRNAs declined gradually and showed biphasic kinetics. Serum HBV pgRNA levels in patients treated with entecavir became negative later than those treated with adefovir or lamivudine. Patients who remain positive for HBV pgRNA after 9 years of treatment may have higher viral transcription efficiencies. The reverse transcription efficiency of hepatitis B e-antigen (HBeAg)-positive patients was higher than that of HBeAg-negative patients at baseline and showed no difference after 24-week nucleos(t)ide analog treatment. The trajectory of serum HBV pgRNA-negative transformation differs in patients with different characteristics. Long-term dynamic monitoring of serum HBV pgRNA levels has significance in hepatitis B treatment.
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http://dx.doi.org/10.3389/fmed.2022.851717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099358PMC
April 2022

A New Diazabenzo[k]fluoranthene-based D-A Conjugated Polymer Donor for Efficient Organic Solar Cells.

Macromol Rapid Commun 2022 May 13:e2200276. Epub 2022 May 13.

Key Laboratory for Material Chemistry of Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.

The development of wide-bandgap polymer donors having complementary absorption and compatible energy levels with near-infared (NIR) absorbing nonfullerene acceptors is highly important for realizing high-performance organic solar cells (OSCs). Herein, a new thiophene-fused diazabenzo[k]fluoranthene derivative has been successfully synthesized as the electron-deficient unit to construct an efficient donor-acceptor (D-A) type alternating copolymer donor, namely PABF-Cl, using the chlorinated benzo[1,2-b:4,5-b']dithiophene as the copolymerization unit. PABF-Cl exhibits a wide optical bandgap of 1.93 eV, a deep highest occupied molecular level of -5.36 eV, and efficient hole transport. As a result, OSCs with the best power conversion efficiency of 11.8% has been successfully obtained by using PABF-Cl as the donor to blend with a NIR absorbing BTP-eC9 acceptor. Our work thus provides a new design of electron-deficient unit for constructing high performance D-A type polymer donors. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/marc.202200276DOI Listing
May 2022

FasL microgels induce immune acceptance of islet allografts in nonhuman primates.

Sci Adv 2022 May 13;8(19):eabm9881. Epub 2022 May 13.

Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.

Islet transplantation to treat insulin-dependent diabetes is greatly limited by the need for maintenance immunosuppression. We report a strategy through which cotransplantation of allogeneic islets and streptavidin (SA)-FasL-presenting microgels to the omentum under transient rapamycin monotherapy resulted in robust glycemic control, sustained C-peptide levels, and graft survival in diabetic nonhuman primates for >6 months. Surgical extraction of the graft resulted in prompt hyperglycemia. In contrast, animals receiving microgels without SA-FasL under the same rapamycin regimen rejected islet grafts acutely. Graft survival was associated with increased number of FoxP3 cells in the graft site with no significant changes in T cell systemic frequencies or responses to donor and third-party antigens, indicating localized tolerance. Recipients of SA-FasL microgels exhibited normal liver and kidney metabolic function, demonstrating safety. This localized immunomodulatory strategy succeeded with unmodified islets and does not require long-term immunosuppression, showing translational potential in β cell replacement for treating type 1 diabetes.
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http://dx.doi.org/10.1126/sciadv.abm9881DOI Listing
May 2022

GSK3 Exacerbates Myocardial Ischemia/Reperfusion Injury by Inhibiting Myc.

Oxid Med Cell Longev 2022 29;2022:2588891. Epub 2022 Apr 29.

Department of Endocrinology, The Southwest Hospital of Third Military Medical University, Chongqing 400038, China.

Myocardial ischemia/reperfusion (MI/R) injury is a life-threatening disease with high morbidity and mortality. Herein, the present study is conducted to explore the regulatory mechanism of GSK3 in MI/R injury regarding cardiomyocyte apoptosis and oxidative stress. The MI/R injury mouse model and hypoxic reoxygenation (H/R) cell model were established. The expression pattern of GSK3, FTO, KLF5, and Myc was determined followed by their relation validation. Next, loss-of-function experiments were implemented to verify the effect of GSK3/FTO/KLF5/Myc on cardiomyocyte apoptosis and oxidative stress in the MI/R injury mouse model and H/R cell model. High expression of GSK3 and low expression of FTO, KLF5, and Myc were observed in the MI/R injury mouse model and H/R cell model. GSK3 promoted phosphorylation of FTO and KLF5, thus increasing the ubiquitination degradation of FTO and KLF5. A decrease of FTO and KLF5 was able to downregulate Myc expression, resulting in enhanced cardiomyocyte apoptosis and oxidative stress. These data together supported the crucial role that GSK3 played in facilitating cardiomyocyte apoptosis and oxidative stress so as to accelerate MI/R injury, which highlights a promising therapeutic strategy against MI/R injury.
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http://dx.doi.org/10.1155/2022/2588891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076327PMC
May 2022

Color-preserving passive radiative cooling for an actively temperature-regulated enclosure.

Light Sci Appl 2022 May 4;11(1):122. Epub 2022 May 4.

State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, 310027, Hangzhou, China.

Active temperature control devices are widely used for the thermal management of enclosures, including vehicles and buildings. Passive radiative cooling has been extensively studied; however, its integration with existing actively temperature regulated and decorative enclosures has slipped out of the research at status quo. Here, we present a photonic-engineered dual-side thermal management strategy for reducing the active power consumption of the existing temperature-regulated enclosure without sacrificing its aesthetics. By coating the exterior and interior of the enclosure roof with two visible-transparent films with distinctive wavelength-selectivity, simultaneous control over the energy exchange among the enclosure with the hot sun, the cold outer space, the atmosphere, and the active cooler can be implemented. A power-saving of up to 63% for active coolers of the enclosure is experimentally demonstrated by measuring the heat flux compared to the ordinary enclosure when the set temperature is around 26°C. This photonic-engineered dual-side thermal management strategy offers facile integration with the existing enclosures and represents a new paradigm toward carbon neutrality.
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http://dx.doi.org/10.1038/s41377-022-00810-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068694PMC
May 2022

Inhibition of APE1 Expression Enhances the Antitumor Activity of Olaparib in Triple-Negative Breast Cancer.

Evid Based Complement Alternat Med 2022 13;2022:6048017. Epub 2022 Apr 13.

Cancer Center, Daping Hospital, Army Medical University, Chongqing 400042, China.

Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer that is prone to recurrence and metastasis. Because of the lack of expression of estrogen receptor (ER) and progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) in TNBC, treatment methods are greatly limited. In this study, the proliferation inhibition and apoptosis-inducing effects of PARP1 inhibitors in TNBC breast cancer cells and in vivo xenograft animal models were examined to investigate the molecular role of APE1 in PARP1-targeted therapy. In TNBC patients, the expression of APE1 and PARP1 were positively correlated, and high expression of APE1 and PARP1 was associated with poor survival of TNBC. Our results indicated that knockdown APE1 could increase the sensitivity of olaparib in the treatment of TNBC. In conclusion, the results of this study will not only clarify the molecular role of APE1 in PARP1-targeted therapy for TNBC but also provide a theoretical basis for the future clinical application of targeting APE1 and PARP1 in the treatment of refractory TNBC.
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http://dx.doi.org/10.1155/2022/6048017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020940PMC
April 2022

Production of Red Pigments by a Newly Isolated Strain with LED Stimulation for Screen Printing.

Indian J Microbiol 2022 Jun 1;62(2):280-292. Epub 2022 Mar 1.

Department of Clinical and Diagnostic Sciences, School of Health Sciences, Oakland University, 433 Meadowbrook Road, Rochester, MI 48309 USA.

Microbial pigments have been widely applied to printing in food, textile, and paper industries as a sustainable alternative to synthetic dyes. Herein, we isolated a novel strain with a strong ability to produce red pigments. We further studied pigment production conditions, stability, screen printing application, and bioactivities. Our results showed that sucrose was a favourable carbon source and the addition of l-histidine significantly enhanced the production of red pigments. Pigment production was strictly photo-regulated with effective wavelengths around 450 nm (blue light). We mixed the red pigments with cellulosic materials and explored their application potentials for screen printing on paper, cotton fabrics, and polymeric carriers. The printing density was significantly improved from 0.3 to 0.7 by overlay printing. pigments could be stably stored at pH 5-11, temperature - 10 to 70 °C, and redox potential - 200 to 300 mV. Moreover, the stable ranges were extended to pH 1-11 and temperature over 100 °C after screen-printed on paper. The red pigments exhibited antioxidant activity towards 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (IC 10.4 mg L in solution). Our results further indicated the red pigments by was environmentally friendly based on acetylcholinesterase activity assay.

Supplementary Information: The online version contains supplementary material available at 10.1007/s12088-022-01008-x.
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http://dx.doi.org/10.1007/s12088-022-01008-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980182PMC
June 2022

Manipulating Reaction Energy Coordinate Landscape of Mechanochemical Diaza-Cope Rearrangement.

Molecules 2022 Apr 15;27(8). Epub 2022 Apr 15.

School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Chiral vicinal diamines, a unique class of optically-active building blocks, play a crucial role in material design, pharmaceutical, and catalysis. Traditionally, their syntheses are all solvent-based approaches, which make organic solvent an indispensable part of their production. As part of our program aiming to develop chemical processes with reduced carbon footprints, we recently reported a highly practical and environmentally-friendly synthetic route to chiral vicinal diamines by solvent-free mechanochemical diaza-Cope rearrangement. We herein showed that a new protocol by co-milling with common laboratory solid additives, such as silica gel, can significantly enhance the efficiency of the reaction, compared to reactions in the absence of additives. One possible explanation is the Lewis acidic nature of additives that accelerates a key Schiff base formation step. Reaction monitoring experiments tracing all the reaction species, including reactants, intermediates, and product, suggested that the reaction profile is distinctly different from ball-milling reactions without additives. Collectively, this work demonstrated that additive effect is a powerful tool to manipulate a reaction pathway in mechanochemical diazo-Cope rearrangement pathway, and this is expected to find broad interest in organic synthesis using mechanical force as an energy input.
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http://dx.doi.org/10.3390/molecules27082570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027841PMC
April 2022

Improve Anti-Tuberculosis Drugs-Induced Intestinal Adverse Reactions in Rat by Modulating Gut Microbiota and Short-Chain Fatty Acids.

Nutrients 2022 Apr 17;14(8). Epub 2022 Apr 17.

Department of Nutrition and Food Hygiene, School of Public Health, Qingdao University, Qingdao 266021, China.

The adverse effects of anti-tuberculosis (TB) drugs in the intestines were related to alteration of the intestinal microbiota. However, there was less information about microbial metabolism on the adverse reactions. This study aimed to explore whether could regulate gut microbiota or short-chain fatty acids (SCFAs) disorders to protect intestinal adverse reactions induced by isoniazid (H) and rifampicin (R). Male Wistar rats were given low and high doses of two hours before daily administration of anti-TB drugs. After 42 days, colon tissue and blood were collected for analysis. The feces at two-week and six-week were collected to analyze the microbial composition and the content of SCFAs in colon contents was determined. Supplementation of increased the proportion of intestinal goblet cells induced by H and R ( < 0.05). In addition, HR also reduced the level of mucin-2 ( < 0.05), and supplementation of restored. After two weeks of HR intervention, a decrease in OTUs, diversity index, the abundance of , , and , and an increase of the abundance of and , were observed compared with the control group ( all < 0.05). These indices in intervention groups were similar to the HR group. Six-week intervention resulted in a dramatic reduction of , butyric acid, valeric acid and hexanoic acid, while an increase of and ( all < 0.05). Pretreatment with significantly increased the content of hexanoic acid compared with HR group ( < 0.05). might prevent intestinal injury induced by anti-tuberculosis drugs by regulating gut microbiota and SCFAs metabolism.
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http://dx.doi.org/10.3390/nu14081668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032531PMC
April 2022

In Reply to Carpenter et al.

Int J Radiat Oncol Biol Phys 2022 05;113(1):234-235

Exercise Medicine Research Institute, Edith Cowan University, Joondalup, WA, Australia; School of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia.

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http://dx.doi.org/10.1016/j.ijrobp.2022.01.041DOI Listing
May 2022

GPR177 in A-fiber sensory neurons drives diabetic neuropathic pain via WNT-mediated TRPV1 activation.

Sci Transl Med 2022 Apr 6;14(639):eabh2557. Epub 2022 Apr 6.

Department of Neurobiology and Department of Anesthesiology of First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.

Diabetic neuropathic pain (DNP) is a common and devastating complication in patients with diabetes. The mechanisms mediating DNP are not completely elucidated, and effective treatments are lacking. A-fiber sensory neurons have been shown to mediate the development of mechanical allodynia in neuropathic pain, yet the molecular basis underlying the contribution of A-fiber neurons is still unclear. Here, we report that the orphan G protein-coupled receptor 177 (GPR177) in A-fiber neurons drives DNP via WNT5a-mediated activation of transient receptor potential vanilloid receptor-1 (TRPV1) ion channel. GPR177 is mainly expressed in large-diameter A-fiber dorsal root ganglion (DRG) neurons and required for the development of DNP in mice. Mechanistically, we found that GPR177 mediated the secretion of WNT5a from A-fiber DRG neurons into cerebrospinal fluid (CSF), which was necessary for the maintenance of DNP. Extracellular perfusion of WNT5a induced rapid currents in both TRPV1-expressing heterologous cells and nociceptive DRG neurons. Computer simulations revealed that WNT5a has the potential to bind the residues at the extracellular S5-S6 loop of TRPV1. Using a peptide able to disrupt the predicted WNT5a/TRPV1 interaction suppressed DNP- and WNT5a-induced neuropathic pain symptoms in rodents. We confirmed coexpression in human DRG neurons and WNT5A secretion in CSF from patients with DNP. Thus, our results reveal a role for WNT5a as an endogenous and potent TRPV1 agonist, and the GPR177-WNT5a-TRPV1 axis as a driver of DNP pathogenesis in rodents. Our findings identified a potential analgesic target that might relieve neuropathic pain in patients with diabetes.
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http://dx.doi.org/10.1126/scitranslmed.abh2557DOI Listing
April 2022

The Prognostic Impact of Tumor Location in pT3N0M0 Upper Urinary Tract Urothelial Carcinoma: A Retrospective Cohort Study.

Front Oncol 2022 15;12:850874. Epub 2022 Mar 15.

Department of Urology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.

Background: We aimed to evaluate the impact of tumor location on cancer outcomes in patients with pT3N0M0 upper tract urothelial carcinoma (UTUC) treated with radical nephroureterectomy (RNU) with bladder cuff excision.

Materials And Methods: We retrospectively reviewed 302 patients with pT3N0M0 UTUC who underwent RNU with bladder cuff excision at our institution between 2005 and 2019, including 191 renal pelvis tumors and 111 ureteral tumors. Clinicopathologic characteristics were compared between renal pelvis and ureter urothelial carcinomas. Multivariate Cox proportional hazard regression was used to assess the association between outcomes and clinical factors. Outcomes of interest included intravesical recurrence-free survival (IVRFS), local recurrence-free survival (LRFS), distant metastasis-free survival (DMFS), and cancer-specific survival (CSS), which were measured using the Kaplan-Meier curve with a log-rank test.

Results: A total of 302 patients underwent RNU with bladder cuff excision. During the median follow-up of 42.7 months, 70 (23.2%), 95 (31.5%), and 99 (32.8%) patients experienced intravesical recurrence, local recurrence, and distant metastasis, respectively. Seventy (23.2%) patients died from UTUC. Multivariate Cox regression analysis showed that tumor location was an independent predictor of local recurrence (HR = 2.05, p = 0.001), with borderline independent significance in intravesical recurrence (HR = 1.54, p = 0.074) and distant metastasis (HR = 1.45, p = 0.08). Kaplan-Meier analysis showed that ureter tumors had a worse 5-year local recurrence (log-rank p < 0.001) and borderline worse 5-year intravesical recurrence (log-rank p = 0.055) and 5-year distant metastasis (log-rank p = 0.073).

Conclusion: Ureter tumors seem to be associated with worse oncological outcomes, especially with local recurrence in UTUC. Further large and long-term studies are warranted for investigating biological differences based on tumor location.
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http://dx.doi.org/10.3389/fonc.2022.850874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964942PMC
March 2022

Lesion-attention pyramid network for diabetic retinopathy grading.

Artif Intell Med 2022 04 25;126:102259. Epub 2022 Feb 25.

Department of Mechanical and Industrial Engineering, Faculty of Engineering, Norwegian University of Science and Technology, Trondheim 7034, Norway. Electronic address:

As one of the most common diabetic complications, diabetic retinopathy (DR) can cause retinal damage, vision loss and even blindness. Automated DR grading technology has important clinical significance, which can help ophthalmologists achieve rapid and early diagnosis. With the popularity of deep learning, DR grading based on the convolutional neural networks (CNNs) has become the mainstream method. Unfortunately, although the CNN-based method can achieve satisfactory diagnostic accuracy, it lacks significant clinical information. In this paper, a lesion-attention pyramid network (LAPN) is presented. The pyramid network integrates the subnetworks with different resolutions to get multi-scale features. In order to take the lesion regions in the high-resolution image as the diagnostic evidence, the low-resolution network calculates the lesion activation map (using the weakly-supervised localization method) and guides the high-resolution network to concentrate on the lesion regions. Furthermore, a lesion attention module (LAM) is designed to capture the complementary relationship between the high-resolution features and the low-resolution features, and to fuse the lesion activation map. Experiment results show that the proposed scheme outperforms other existing approaches, and the proposed method can provide lesion activation map with lesion consistency as an additional evidence for clinical diagnosis.
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http://dx.doi.org/10.1016/j.artmed.2022.102259DOI Listing
April 2022

Development and Validation of a Nomogram to Predict Cancer-Specific Survival for Middle-Aged Patients With Early-Stage Hepatocellular Carcinoma.

Front Public Health 2022 28;10:848716. Epub 2022 Feb 28.

General Surgery Center, The General Hospital of Western Theater, Chengdu, China.

Background: Hepatocellular carcinoma is a common cause of death in middle-aged patients. We aimed to construct a new nomogram to predict cancer-specific survival (CSS) in middle-aged patients with hepatocellular carcinoma at an early stage.

Method: We collected clinicopathological information on early middle-aged patients with hepatocellular carcinoma from the SEER database. Univariate and multivariate Cox regression models were used to screen the independent risk factors for prognosis. These risk factors were used to construct predictions of CSS in patients with hepatocellular carcinoma. Consistency index (C- index), calibration curve, area under the receiver operating curve (AUC) were used. A decision analysis curve (DCA) was used to evaluate the clinical utility of the predictive model.

Results: A total of 6,286 patients with hepatocellular carcinoma in early middle age were enrolled. Univariate and multivariate Cox regression analysis showed that sex, marriage, race, histological tumor grade, T stage, surgery, chemotherapy, AFP, and tumor size were independent risk factors for prognosis. All independent risk factors were included in the nomogram to predict CSS at 1-, 3-, and 5-years in early middle age patients with hepatocellular carcinoma. In the training cohort and validation cohort, the C-index of the prediction model was 0.728 (95%CI: 0.716-0.740) and 0.733 (95%CI: 0.715-0.751), respectively. The calibration curve showed that the predicted value of the prediction model is highly consistent with the observed value. AUC also suggested that the model has good discrimination. DCA suggested that the nomogram had better predictive power than T staging.

Conclusion: We constructed a new nomogram to predict CSS in middle-aged patients with early-stage hepatocellular carcinoma. This prediction model has good accuracy and reliability, which can help patients and doctors to judge prognosis and make clinical decisions.
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http://dx.doi.org/10.3389/fpubh.2022.848716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918547PMC
April 2022

Expanding the DNA-encoded library toolbox: identifying small molecules targeting RNA.

Nucleic Acids Res 2022 Mar 14. Epub 2022 Mar 14.

HitGen Inc., Shuangliu District, Chengdu, China.

DNA-encoded library (DEL) technology is a powerful tool for small molecule identification in drug discovery, yet the reported DEL selection strategies were applied primarily on protein targets in either purified form or in cellular context. To expand the application of this technology, we employed DEL selection on an RNA target HIV-1 TAR (trans-acting responsive region), but found that the majority of signals were resulted from false positive DNA-RNA binding. We thus developed an optimized selection strategy utilizing RNA patches and competitive elution to minimize unwanted DNA binding, followed by k-mer analysis and motif search to differentiate false positive signal. This optimized strategy resulted in a very clean background in a DEL selection against Escherichia coli FMN Riboswitch, and the enriched compounds were determined with double digit nanomolar binding affinity, as well as similar potency in functional FMN competition assay. These results demonstrated the feasibility of small molecule identification against RNA targets using DEL selection. The developed experimental and computational strategy provided a promising opportunity for RNA ligand screening and expanded the application of DEL selection to a much wider context in drug discovery.
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http://dx.doi.org/10.1093/nar/gkac173DOI Listing
March 2022

Depression duration and risk of incident cardiovascular disease: A population-based six-year cohort study.

J Affect Disord 2022 05 10;305:188-195. Epub 2022 Mar 10.

Department of Social Work and Social Administration, The University of Hong Kong, Hong Kong, China; Sau Po Centre on Ageing, The University of Hong Kong, Hong Kong, China.

Background: Depression symptoms are significantly associated with an increased risk of cardiovascular disease (CVD). However, understanding of the magnitude of the association between depression duration and risk of CVD is limited. Therefore, we aimed to assess whether a longer duration of exposure to depression is associated with a higher risk of new-onset CVD.

Methods: We conducted a territory-wide retrospective cohort study among patients (≥ 10 years old) with depression diagnosed between January and December 2014 in Hong Kong. The observation period spanned January 1, 2014, to December 31, 2019, and all participants had no CVD at baseline. Incidence of CVD was calculated. We used Cox proportional hazard regression to adjust confounders and estimate hazard ratios of CVD risk.

Results: Among 11,651 participants with depression, 1306 (11.2%) individuals developed CVD. Multi-adjusted models showed individuals with depression duration of 2-5 years (Hazard Ratios [HRs]: 1.38 [95% confidence interval (CI): 1.19-1.60]) and ≥6 years (1.45 [1.25-1.68]) had a significantly escalated risk of developing CVD, compared to those with depression within one year. Stratified analyses indicated that the association was prominent in women and those under 65 years old.

Limitations: Lack of depression severity information and the small sample size in some subgroup analyses.

Conclusions: Longer exposure to depression is associated with significant increased risk of CVD. The interplay between mental and vascular health emphasizes the need for CVD prevention in patients with long-term depression.
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http://dx.doi.org/10.1016/j.jad.2022.03.005DOI Listing
May 2022

Construction of Graphene-Based "In-Paper" 3D Interdigital Microelectrodes for High Performance Metal-Free Flexible Supercapacitors.

Small Methods 2022 May 6;6(5):e2101454. Epub 2022 Mar 6.

Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, P. R. China.

Flexible micro-supercapacitors (MSCs) are promising power sources of portable/wearable electronic devices. Electrodes are the key components determining performance of the MSCs, but it still remains a big challenge in either materials or fabrication methods to achieve both high charge storage capability and robust mechanical flexibility. Herein, a novel water-cooling assisted selective laser ablation (WASLA) technique is demonstrated for scale-fabrication of "embedded-in-paper" 3D graphene-cellulose composite interdigital electrodes (3D GCCIEs) in a mask-free and chemical-free manner. The obtained electrodes are endowed with 3D charge storage geometry, high electrical conduction, freely designed patterns, and the inherent advantages of paper substrate. Therefore, the 3D GCCIEs-based MSC exhibits excellent overall performance including large specific capacitances, high rate performance, impressive cyclic stability, and remarkable mechanical flexibility. Moreover, metal-free 3D GCCIE-MSC integrated arrays with diverse shapes composed of linear/curved interdigital electrodes are also fabricated, and a letter-shaped MSC array successfully lit a light emitting diode light in both flat and folded status demonstrating excellent device flexibility. The as-fabricated 3D GCCIE-MSCs have shown great application potential as power sources of flexible electronic devices, and the WASLA method proves to be an effective strategy for scale-manufacturing high performance paper-based charge storage devices not limited to supercapacitors.
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http://dx.doi.org/10.1002/smtd.202101454DOI Listing
May 2022

Adverse Events Reporting of Clinical Trials in Exercise Oncology Research (ADVANCE): Protocol for a Scoping Review.

Front Oncol 2022 16;12:841266. Epub 2022 Feb 16.

Exercise Medicine Research Institute, Edith Cowan University, Joondalup, WA, Australia.

Introduction: Adequate, transparent, and consistent reporting of adverse events (AEs) in exercise oncology trials is critical to assess the safety of exercise interventions for people following a cancer diagnosis. However, there is little understanding of how AEs are reported in exercise oncology trials. Thus, we propose to conduct a scoping review to summarise and evaluate current practice of reporting of AEs in published exercise oncology trials with further exploration of factors associated with inadequate reporting of AEs. The study findings will serve to inform the need for future research on standardisation of the definition, collection, and reporting of AEs for exercise oncology research.

Materials And Methods: The ADVANCE (ADverse eVents reporting of clinicAl trials iN exerCise oncology rEsearch) study will be conducted and reported following the PRISMA extension for scoping reviews guideline. Any type of clinical trial involving an exercise intervention in people living with and beyond cancer with a full-text report in English will be included. Six electronic databases (Embase, PubMed, Google Scholar, Web of Science Core Collection, SPORTDiscus, and CINAHL Plus) will be searched for studies. Two independent review authors will assess eligibility of identified studies, chart data using pre-established extraction forms, and evaluate adequacy of reporting of AEs-related data against a 20-item scoring checklist derived from the CONSORT (Consolidated Standards of Reporting Trials) harms extension. We will summarise results using descriptive and inferential analysis methods.

Ethics And Dissemination: No ethics approval will be required to conduct the ADVANCE study owing to inclusion of only published data. The study results will be disseminated publications in peer-reviewed journals and presentations at national and internationa  conferences.

Systematic Review Registration: Open Science Framework: https://osf.io/NXEJD/ (doi:10.17605/OSF.IO/NXEJD).
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http://dx.doi.org/10.3389/fonc.2022.841266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889497PMC
February 2022

Reliability coefficients for multiple group item response theory models.

Br J Math Stat Psychol 2022 05 1;75(2):395-410. Epub 2022 Mar 1.

University of Oslo, Norway.

Reliability of scores from psychological or educational assessments provides important information regarding the precision of measurement. The reliability of scores is however population dependent and may vary across groups. In item response theory, this population dependence can be attributed to differential item functioning or to differences in the latent distributions between groups and needs to be accounted for when estimating the reliability of scores for different groups. Here, we introduce group-specific and overall reliability coefficients for sum scores and maximum likelihood ability estimates defined by a multiple group item response theory model. We derive confidence intervals using asymptotic theory and evaluate the empirical properties of estimators and the confidence intervals in a simulation study. The results show that the estimators are largely unbiased and that the confidence intervals are accurate with moderately large sample sizes. We exemplify the approach with the Montreal Cognitive Assessment (MoCA) in two groups defined by education level and give recommendations for applied work.
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http://dx.doi.org/10.1111/bmsp.12269DOI Listing
May 2022

3D Bioprinted GelMA-Nanoclay Hydrogels Induce Colorectal Cancer Stem Cells Through Activating Wnt/β-Catenin Signaling.

Small 2022 May 1;18(18):e2200364. Epub 2022 Mar 1.

Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, P. R. China.

Cancer stem cells (CSCs) are a rare cell population in tumors that are responsible for tumor recurrence and metastasis. They are a priority as therapeutic targets, however, assays targeting CSCs have been limited by expanding and maintaining CSCs in vitro. Here, the authors find that gelatin methacryloyl (GelMA)-nanoclay hybrid hydrogels can induce and enrich colorectal CSCs assisted by three-dimensional (3D) bioprinting. The presence of the nanoclay increases the printability, Young's modulus, pore size, and cytocompatibility of the hydrogels. Bioprinted GelMA-nanoclay hydrogels promote the formation of spheroids expressing elevated levels of the stemness markers LGR5, CD133, CD26, and SOX2. Cancer cells grown in GelMA-nanoclay hydrogel possess higher self-renewal and differentiation capacity in vitro and higher tumorigenic capacity in vivo. GelMA-nanoclay hydrogels induce CSCs by stimulating the activation of the Wnt/β-catenin signaling pathway. Further studies demonstrate that spheroids from GelMA-nanoclay hydrogels possess increased stemness, higher consistency, yield, and sensitivity to the anti-CSC compounds compared to the classic CSC-enrichment model. Collectively, this study may provide a valuable biomaterial and method for inducing and enriching CSCs, to facilitate the effective CSC-targeting drug screening.
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http://dx.doi.org/10.1002/smll.202200364DOI Listing
May 2022

Correlative AFM and Scanning Microlens Microscopy for Time-Efficient Multiscale Imaging.

Adv Sci (Weinh) 2022 04 27;9(12):e2103902. Epub 2022 Feb 27.

State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, P. R. China.

With the rapid evolution of microelectronics and nanofabrication technologies, the feature sizes of large-scale integrated circuits continue to move toward the nanoscale. There is a strong need to improve the quality and efficiency of integrated circuit inspection, but it remains a great challenge to provide both rapid imaging and circuit node-level high-resolution images simultaneously using a conventional microscope. This paper proposes a nondestructive, high-throughput, multiscale correlation imaging method that combines atomic force microscopy (AFM) with microlens-based scanning optical microscopy. In this method, a microlens is coupled to the end of the AFM cantilever and the sample-facing side of the microlens contains a focused ion beam deposited tip which serves as the AFM scanning probe. The introduction of a microlens improves the imaging resolution of the AFM optical system, providing a 3-4× increase in optical imaging magnification while the scanning imaging throughput is improved ≈8×. The proposed method bridges the resolution gap between traditional optical imaging and AFM, achieves cross-scale rapid imaging with micrometer to nanometer resolution, and improves the efficiency of AFM-based large-scale imaging and detection. Simultaneously, nanoscale-level correlation between the acquired optical image and structure information is enabled by the method, providing a powerful tool for semiconductor device inspection.
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http://dx.doi.org/10.1002/advs.202103902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036010PMC
April 2022

Biological activity of a vascular endothelial cell-hydroxyapatite orbital implant complex: An experimental study.

Exp Ther Med 2022 Mar 18;23(3):227. Epub 2022 Jan 18.

Department of Ocular Oncology and Ocular Trauma, Affiliated Eye Hospital of Nanchang University, Jiangxi Research Institute of Ophthalmology and Visual Sciences, Key Laboratory of Ophthalmology of Jiangxi Province, Nanchang, Jiangxi 330006, P.R. China.

The reduction in postoperative complications is a considerable concern after orbital reparation and reconstruction. Selecting the appropriate scaffold materials to improve the survival rates of the seeded cells is a challenge in tissue engineering. The aim of the present study was to evaluate the biological activity of a vascular endothelial cell-hydroxyapatite orbital complex, which was constructed with tissue engineering and used as an implant after enucleation of the eyeNew Zealand white rabbits were randomly divided into two groups that underwent hydroxyapatite orbital implant surgery in the right eye. The primary orbital microvascular endothelial cells were collected from the microvascular tissue and subsequently cultured. Then, hydroxyapatite ocular implants were cultured with vascular endothelial cells in the endothelial cell (EC) group, and implants were cultured without vascular endothelial cells in the blank group. Characterization of the cells was performed with immunofluorescence staining and a Transwell migration and cell tube formation assay. The levels of interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF) in the rabbit conjunctiva were measured with an ELISA. The results showed that the levels of IL-8 were decreased in the EC group and increased in the blank group. The levels of VEGF were increased in the EC group when compared to the blank group with statistical significance. The average depth of the fibrovascular tissue was obviously thicker in the EC group compared with that found in the blank group. These findings suggest that the vascular endothelial cell-hydroxyapatite orbital implant complex may be an effective strategy with which to accelerate vascularization and reduce complications of infection with satisfactory biological activity.
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http://dx.doi.org/10.3892/etm.2022.11152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815058PMC
March 2022

Multimorbidity Patterns and 5-Year Mortality in Institutionalized Older Adults.

J Am Med Dir Assoc 2022 Feb 24. Epub 2022 Feb 24.

Department of Cardiovascular, Endocrine-Metabolic Diseases and Aging, Istituto Superiore di Sanità, Rome, Italy.

Objectives: The aim was to characterize multimorbidity patterns in a large sample of older individuals living in nursing homes (NHs) and to investigate their association with mortality, also considering the effect of functional status.

Design: Observational and retrospective study.

Setting And Participants: We analyzed data on 4131 NH residents in Italy, aged 60 years and older, assessed through the interRAI long-term care facility instrument. Entry date was between 2014 and 2018, and participants were followed until 2019.

Methods: Multimorbidity patterns were identified through principal component analysis; for the identified components, subjects were stratified in quintiles (Q) with respect to their loading values, with the higher quantiles indicating greater expression of the component's pattern. Their association [hazard ratio (HR) and 95% CI] with mortality was tested in Cox regression models. Analyses were stratified by disability status.

Results: Four patterns of multimorbidity were identified: (1) heart diseases; (2) dementia and sensory impairments; (3) heart, respiratory, and psychiatric diseases; and (4) diabetes, musculoskeletal, and vascular diseases. For the heart diseases pattern [HR Q5 vs Q1 = 1.83 (1.53-2.20)] and the dementia and sensory impairments pattern [HR Q5 vs Q1 = 1.23 (1.06-1.42)], as the specific multimorbidity expression increases, the risk of mortality increases. On stratifying by disability status, the association between the multimorbidity patterns and mortality was not always present.

Conclusions And Implications: Different multimorbidity patterns are differentially associated with mortality in older residents of NHs, confirming that multimorbidity's prognosis is strictly dependent on the underlying disease combinations. This knowledge may be useful to implement personalized preventive and therapeutic care pathways for institutionalized older adults, which respond to individuals' health needs.
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http://dx.doi.org/10.1016/j.jamda.2022.01.067DOI Listing
February 2022

High-precision micro-displacement measurement method based on alternately oscillating optoelectronic oscillators.

Opt Express 2022 Feb;30(4):5644-5656

We propose a high-precision micro-displacement measurement method based on alternately oscillating optoelectronic oscillators (OEOs). This method uses a reference loop to compensate for the change in the measuring loop length except for the displacement to be measured. Therefore, self-calibration is realized without using a phase-locked loop to control the loop length, greatly simplifying the system. The measurement range is 20 mm, and the measurement precision is <300 nm, which is limited by the incomplete consistency between the reference and the measuring loops, with the exception of the displacement to be measured and environmental disturbances resulting from the spatial optical path.
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http://dx.doi.org/10.1364/OE.450812DOI Listing
February 2022

Targeting the epigenetic processes to enhance antitumor immunity in small cell lung cancer.

Semin Cancer Biol 2022 Feb 18. Epub 2022 Feb 18.

School of Medicine, Chongqing University, Chongqing 400030, China. Electronic address:

Dysregulation of the epigenetic processes, such as DNA methylation, histone modifications, and modulation of chromatin states, drives aberrant transcription that promotes initiation and progression of small cell lung cancer (SCLC). Accumulating evidence has proven crucial roles of epigenetic machinery in modulating immune cell functions and antitumor immune response. Epigenetics-targeting drugs such as DNA methyltransferase inhibitors, histone deacetylase inhibitors, and histone methyltransferase inhibitors involved in preclinical and clinical trials may trigger antitumor immunity. Herein, we summarize the impact of epigenetic processes on tumor immunogenicity and antitumor immune cell functions in SCLC. Furthermore, we review current clinical trials of epigenetic therapy against SCLC and the mechanisms of epigenetic inhibitors to boost antitumor immunity. Eventually, we discuss the opportunities of developing therapeutic regimens combining epigenetic agents with immunotherapy for SCLC.
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http://dx.doi.org/10.1016/j.semcancer.2022.02.018DOI Listing
February 2022

[Clinical treatment of dorsal avulsion fracture of the capitellum combined with medial or posterior medial dislocation of the elbow joint].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2022 Feb;36(2):149-154

Department of Orthopedics, Xiangya Hospital, Central South University, Changsha Hunan, 410008, P. R. China.

Objective: To analyze the possible injury mechanisms in patients with dorsal avulsion fracture of the capitellum combined with medial or posterior medial dislocation of the elbow joint, and to discuss their treatment and prognosis.

Methods: Retrospective analysis was made on the clinical data of 4 patients with dorsal avulsion fracture of the capitellum combined with medial or posterior medial dislocation of the elbow joint admitted between September 2014 and September 2020, including 3 males and 1 female with an average age of 20.7 years (range, 13-32 years). There were 2 cases of dorsal avulsion fracture of the capitellum combined with medial dislocation of the elbow joint and 2 cases of dorsal avulsion fracture of the capitellum and anterior medial fracture of the coronoid process combined with posterior medial subluxation of the elbow joint. Closed reduction was performed in 3 patients with fresh fracture combined with dislocation, then 2 cases were fixed with tension band and 1 case was fixed with tension band combined with Acumed coronoid anatomic plate. And in patient with old fracture nonunion, the coronoid process was fixed with 1 screw, then the humeral sclerotic bone mass was removed, and finally the lateral collateral ligament was repaired and a hinged external fixator was added.

Results: All the incisions healed by first intention without early complications such as infection or peripheral nerve injury. The 4 patients were followed up 13-30 months (mean, 20.8 months). The fractures all healed with a healing time of 70-90 days (mean, 79.5 days). At 6 months after operation, heterotopic ossification was seen in the posterior aspect of the right elbow joint in 1 case, and the alkaline phosphatase level was normal (67 U/L); the tension band was removed to clear the heterotopic ossification and the elbow joint was released. The rest of the patients had no heterotopic ossification. At last follow-up, all patients had good functional recovery of the elbow joint, with a Mayo score of 85-100 (mean, 92.5), and the excellent and good rate was 100%. The elbow flexion range of motion was 120°-135°, the extension range of motion was 10°-20°, and the pronation and supination range of motion were all 75°-85°.

Conclusion: Dorsal avulsion fractures of the capitellum combined with medial or posterior medial dislocation of the elbow may be due to simple varus stress. If an anteromedial coronoid facet fracture also occurs, it may be for the varus posteromedial rotatory instability, which is the opposite mechanism to that of an Osborne-Cotterill lesion. For fresh dorsal avulsion fractures of the capitellum, tension band fixation can be used with good results.
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http://dx.doi.org/10.7507/1002-1892.202108029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863534PMC
February 2022

Cervix chip mimicking cervical microenvironment for quantifying sperm locomotion.

Biosens Bioelectron 2022 May 29;204:114040. Epub 2022 Jan 29.

Shanghai Institute of Cardiovascular Diseases, Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Department of Systems Biology for Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. Electronic address:

As the gate for sperm swimming into the female reproductive tract, cervix is full of cervical mucus, which plays an important role in sperm locomotion. The fact that sperm cannot pass through the cervical mucus-cervix microenvironment will cause the male infertility. However, how the sperm swim across the cervix microenvironment remains elusive. We used hyaluronic acid (HA), a substitute of cervical mucus to mimic cervix microenvironment and designed a cervix chip to study sperm selection and behavior. An accumulation of sperm in HA confirmed that HA served as a reservoir for sperm, similar to cervical mucus. We found that sperm escaping from HA exhibited higher motility than the sperm accessing into HA, suggesting that HA functions as a filter to select sperm with high activity. Our findings construct a practical platform to explore the sophisticated interaction of sperm with cervix microenvironment, with elaborate swimming indicators thus provide a promising cervix chip for sperm selection with kinematic features on-demand. What's more, the cervix chip allows the convenient use in clinical infertility diagnosis, owing to the advantage of simple, fast and high efficiency.
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http://dx.doi.org/10.1016/j.bios.2022.114040DOI Listing
May 2022

Evaluation of the association of a variant in PNPLA3 and TM6SF2 with fibrosis progression in patients with chronic hepatitis C infection after eradication: A retrospective study.

Gene 2022 Apr 7;820:146235. Epub 2022 Feb 7.

Departments of Infectious Diseases, Peking University First Hospital, NO.8, Xishiku Street, Xicheng District, Beijing 100034, People's Republic of China. Electronic address:

The relationship of single nucleotide polymorphisms (SNPs) in patatin-like phospholipase domain containing 3 (PNPLA3) rs738409, transmembrane 6 superfamily member 2 (TM6SF2) rs58542926, and membrane bound O-acyltransferase domain containing 7 (MBOAT7) rs641738 with outcomes in patients with hepatitis C infection (HCV) is unclear. This study aimed to evaluate the association of PNPLA3, TM6SF2, and MBOAT7 with the baseline fibrosis stage and progression of liver fibrosis after HCV eradication with direct antiviral agents (DAAs). A total of 171 patients who received the DAAs at the Peking University First Hospital between June 2015 and June 2020 were included in the retrospective cohort. Transient elastography was used to determine liver stiffness measurements (LSMs) at the baseline, the end of treatment (EOT), 24 weeks after treatment (W24), and the last follow-up (LFU) visit. We used the QIAamp Blood Mini Kit (Qiagen) for whole blood genomic DNA extraction and polymerase chain reaction for PNPLA3, TM6SF2, and MBOAT7 amplification of the target gene. The PNPLA3 rs738409 SNP was associated with the baseline fibrosis stage in multivariate logistic regression analysis adjusted for other factors, and the adjusted odds ratio (OR) for advanced fibrosis (≥F3) at baseline was 2.52 (95% confidence interval[CI] = 1.096-5.794, p = 0.03). The G and GG alleles were predictive of advanced fibrosis (OR = 1.98, 95% CI = 1.021-4.196, p = 0.015; OR = 3.12, 95% CI = 1.572-6.536, p = 0.005). Similarly, the OR of TM6SF2 rs58542926 at baseline was 2.608 (95% CI = 1.081-6.29, p = 0.033). T and TT alleles were predictive of advanced fibrosis (OR = 2.3, 95% CI = 1.005-5.98, p = 0.007; OR = 3.05, 95% CI = 1.32-6.87, p = 0.001). After adjustment, the MBOAT7 rs641738 T plus TT alleles were not independently associated with the baseline fibrosis stage (95% CI = 0.707-2.959, p = 0.312). At the EOT, there were 35 patients and 136 patients in the fibrosis improvement and fibrosis non-improvement group, respectively. Logistic regression analysis showed that the G allele in PNPLA3 rs738409 was associated with fibrosis progression (OR = 2.47, 95% CI = 1.125-5.89, p = 0.003). The GG alleles were predictive of fibrosis progression (OR = 2.95, 95% CI = 1.35-6.35, p = 0.005). Similarly, the ORs of the T and TT alleles in TM6SF2 rs58542926 for fibrosis progression were 1.82 and 2.21, respectively (95% CI = 1.006-5.373, p = 0.045; 95% CI = 1.18-5.75, p = 0.01). At the W24 visit, we found that there was an association between the G allele in PNPLA3 rs738409 and fibrosis progression (OR = 2.218, 95% CI = 1.095-5.631, p = 0.015). Moreover, GG alleles were also predictive for fibrosis progression (OR = 2.558, 95% CI = 1.252-5.15, p = 0.008). Similarly, the OR of T allele and TT alleles in TM6SF2 rs58542926 for fibrosis progression was 2.056 and 2.652 (95% CI = 1.013-5.592, p = 0.038; 95% CI = 1.25-5.956, p = 0.015). For additional affirmation, we surveyed fibrosis progression utilizing the Cox proportional hazards model. G and GG alleles in PNPLA3 rs738409 were associated with an increased risk of progression to advanced fibrosis in multivariate model (hazard ratio [HR]1.566, 95% CI = 1.02-2.575, p = 0.017; and HR2.109, 95% CI = 1.36-3.271, p = 0.001, respectively). Besides, T and TT alleles in TM6SF2 rs58542926 were associated with an increased risk of progression to advanced fibrosis in multivariate model (HR = 1.322, 95% CI = 1.003-1.857, p = 0.045; and HR = 1.855, 95% CI = 1.35-2.765, p = 0.006, respectively). In contrast, rs641738 in MBOAT7 did not show a significant trend in the univariate and multivariate models. The PNPLA3 CG/GG SNP at rs738409 and TM6SF2 CT/TT SNP at rs58542926 were associated with the baseline fibrosis stage and fibrosis progression after HCV eradication with DAAs.
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http://dx.doi.org/10.1016/j.gene.2022.146235DOI Listing
April 2022

Electrospinning [email protected] Core-Shell Nanofibers Based on a Visual Light Transmission Response to Alcohol for Intelligent Packaging.

ACS Appl Mater Interfaces 2022 Feb 7;14(6):8437-8447. Epub 2022 Feb 7.

Key Laboratory of Automobile Materials, Ministry Education, School of Materials Science and Engineering, Jilin University, Changchun 130025, China.

A naked-eye detector based on a rapid transmittance response to alcohol was designed to offer real-time and reusable detection of fruit freshness. To ensure the hydrophobicity of the fibrous membrane and high light transmission response to alcohol, fluorine-rich poly(vinylidene fluoride--hexafluoropropylene) (PVDF-HFP) with a low refractive index was selected as the shell layer, while sodium alginate (SA) and polyvinyl alcohol (PVA) were selected as the core layer for coaxial electrospinning. The core-shell fibrous detector was obtained by treatment with CaCl to form a stable hydrogel and by water flushing to remove PVA. The interior structure of the fiber and its evolution were investigated with increasing SA concentration, which changed from a nonconcentric structure to a core-shell structure. Without SA, nonconcentric structured fibers were obtained due to high flowability and incompatibility between the organic solvent phase of PVDF-HFP and the aqueous phase of PVA. As the SA concentration increased, the enhanced viscosity and surface tension decreased the asymmetric mobility significantly, which competed with the charge attractive forces from the Taylor cone surface, leading to a core-shell structure. The as-spun membranes were opaque due to light scattering at the interface between air and fiber and became light transparent after immersion in a rotten fruit-containing alcohol and acetic acid due to a decreased light loss. The rapidly responsive, reusable fibrous membranes with over 90% light transparency developed here have high potential for application in visual intelligent packaging to monitor the freshness of fruits and vegetables.
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http://dx.doi.org/10.1021/acsami.1c23055DOI Listing
February 2022

Poly(ADP-ribose)polymerase-1 affects hydroquinone-induced aberrant cell cycle and apoptosis through activation of p16/pRb signaling pathway in TK6 cells.

Ecotoxicol Environ Saf 2022 Mar 1;232:113259. Epub 2022 Feb 1.

Institute of Environmental Health, Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China. Electronic address:

Hydroquinone (HQ), a key metabolite of benzene, affects cell cycle and apoptosis. Poly (ADP-ribose) polymerase-1 (PARP-1) plays an important role in DNA damage repair. To explore whether PARP-1 is involved in HQ-induced cell cycle and apoptosis, we assessed the effect of PARP-1 suppression and overexpression on induction of cell cycle and apoptosis analyzed by flow cytometry analysis. We observed that HQ induced aberrant cell cycle progression and apoptosis. We further confirmed that PARP-1 suppression accelerated the cell cycle progression and inhibited cell apoptosis via inhibiting p16/pRb signal pathway after acute HQ exposure, while overexpression of PARP-1 displayed the opposite results. Therefore, we concluded that HQ-induced cell cycle and apoptosis were regulated by PARP-1 through activation of p16/pRb signaling pathway.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113259DOI Listing
March 2022
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