Publications by authors named "Hao Liu"

2,564 Publications

  • Page 1 of 1

Characterization of FtsH Essentiality in Genetic Suppression.

Front Genet 2021 27;12:659220. Epub 2021 Apr 27.

MOE Key Laboratory of Industrial Fermentation Microbiology, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China.

FtsH belongs to the AAA+ ATP-dependent family of proteases, which participate in diverse cellular processes and are ubiquitous among bacteria, chloroplasts, and mitochondria. FtsH is poorly characterized in most organisms, especially compared to other major housekeeping proteases. In the current study, we examined the source of FtsH essentiality in the human oral microbiome species , one of the primary etiological agents of dental caries. By creating a conditionally lethal mutant, we were able to identify a secondary suppressor missense mutation in the gene, encoding the response regulator of the essential VicRK two-component system (TCS). Transcriptomic analysis of the (G195R) mutant revealed significantly reduced expression of 46 genes, many of which were located within the genomic island Tnsmu2, which harbors the mutanobactin biosynthetic gene cluster. In agreement with the transcriptomic data, deletion of the mutanobactin biosynthetic gene cluster suppressed essentiality in . We also explored the role of FtsH in physiology and demonstrated its critical role in stress tolerance, especially acid stress. The presented results reveal the first insights within for the pleiotropic regulatory function of this poorly understood global regulator.
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http://dx.doi.org/10.3389/fgene.2021.659220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112672PMC
April 2021

Intratumoral Foxp3RORγt T cell infiltration determines poor prognosis and immunoevasive contexture in gastric cancer patients.

Cancer Immunol Immunother 2021 May 12. Epub 2021 May 12.

Department of Immunology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

Background: Foxp3RORγt T cells possess both characteristics of regulatory T cells and T helper 17 cells and show significant immunoregulatory functions in autoimmune diseases. However, the role and clinical significance of Foxp3RORγt T cells in gastric cancer remains unclear.

Methods: We enrolled 452 gastric cancer tissue microarray samples and 60 fresh tumor tissue samples from Zhongshan Hospital. The infiltration of Foxp3RORγt T cells and immune contexture were examined by immunohistochemistry and flow cytometry. Survival analyses of patient subgroups were conducted by Kaplan-Meier curves, log-rank test and Cox proportional model.

Results: High infiltration of Foxp3RORγt T cells predicted poor overall survival (P = 0.0222 and 0.0110) and inferior therapeutic response (P = 0.003 for interaction) in gastric cancer. Foxp3RORγt T cells were associated with impaired effective function of CD8 T cells featured by decreased interferon-γ, granzyme B and CD107a expression. Co-evaluation of Foxp3RORγt T cells and CD8 T cells could predict survival outcomes and chemotherapeutic responsiveness more precisely.

Conclusions: We found that Foxp3RORγt T cells could potentially attenuate effective functions of CD8 T cells and led to adverse survival outcomes and inferior chemotherapeutic responsiveness. Moreover, the novel co-evaluation system might be useful for prognosis prediction for appropriate treatment in gastric cancer.

Novelty And Impact Statements: Clinical significance of Foxp3RORγts T cells has not been studied in gastric cancer. Herein, we investigated the prognostic value of Foxp3+RORγt+ T cells in 452 patients. We demonstrated that intratumoral Foxp3RORγt T cell infiltration was a prognostic biomarker for overall survival and the identification of patients might benefit from post-gastrectomy 5-fluorouracil. These findings allow a more precise stratification upon the co-evaluation with CD8 T cells to better clinical management for patients who would benefit from 5-fluorouracil.
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http://dx.doi.org/10.1007/s00262-021-02950-3DOI Listing
May 2021

Disruption of FOXO3a-miRNA feedback inhibition of IGF2/IGF-1R/IRS1 signaling confers Herceptin resistance in HER2-positive breast cancer.

Nat Commun 2021 May 11;12(1):2699. Epub 2021 May 11.

Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, Guangdong, China.

Resistance to Herceptin represents a significant challenge for successful treatment of HER2-positive breast cancer. Here, we show that in Herceptin-sensitive cells, FOXO3a regulates specific miRNAs to control IGF2 and IRS1 expression, retaining basic IGF2/IGF-1R/IRS1 signaling. The basic activity maintains expression of PPP3CB, a subunit of the serine/threonine-protein phosphatase 2B, to restrict FOXO3a phosphorylation (p-FOXO3a), inducing IGF2- and IRS1-targeting miRNAs. However, in Herceptin-resistant cells, p-FOXO3a levels are elevated due to transcriptional suppression of PPP3CB, disrupting the negative feedback inhibition loop formed by FOXO3a and the miRNAs, thereby upregulating IGF2 and IRS1. Moreover, we detect significantly increased IGF2 in blood and IRS1 in the tumors of breast cancer patients with poor response to Herceptin-containing regimens. Collectively, we demonstrate that the IGF2/IGF-1R/IRS1 signaling is aberrantly activated in Herceptin-resistant breast cancer via disruption of the FOXO3a-miRNA negative feedback inhibition. Such insights provide avenues to identify predictive biomarkers and effective strategies overcoming Herceptin resistance.
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http://dx.doi.org/10.1038/s41467-021-23052-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113606PMC
May 2021

Flexibility Effects of a Flapping Mechanism Inspired by Insect Musculoskeletal System on Flight Performance.

Front Bioeng Biotechnol 2021 22;9:612183. Epub 2021 Apr 22.

Graduate School of Engineering, Chiba University, Chiba, Japan.

Flying animals such as insects display great flight performances with high stability and maneuverability even under unpredictable disturbances in natural and man-made environments. Unlike man-made mechanical systems like a drone, insects can achieve various flapping motions through their flexible musculoskeletal systems. However, it remains poorly understood whether flexibility affects flight performances or not. Here, we conducted an experimental study on the effects of the flexibility associated with the flapping mechanisms on aerodynamic performance with a flexible flapping mechanism (FFM) inspired by the flexible musculoskeletal system of insects. Based on wing kinematic and force measurements, we found an appropriate combination of the flexible components could improve the aerodynamic efficiency by increasing the wingbeat amplitude. Results of the wind tunnel experiments suggested that, through some passive adjustment of the wing kinematics in concert with the flexible mechanism, the disturbance-induced effects could be suppressed. Therefore, the flight stability under the disturbances is improved. While the FFM with the most rigid spring was least efficient in the static experiments, the model was most robust against the wind within the range of the study. Our results, particularly regarding the trade-off between the efficiency and the robustness, point out the importance of the passive response of the flapping mechanisms, which may provide a functional biomimetic design for the flapping micro air vehicles (MAVs) capable of achieving high efficiency and stability.
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http://dx.doi.org/10.3389/fbioe.2021.612183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100246PMC
April 2021

Characterization of Frequently Mutated Cancer Genes and Tumor Mutation Burden in Chinese Breast Cancer.

Front Oncol 2021 21;11:618767. Epub 2021 Apr 21.

Department of Breast Cancer, Cancer Center, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Objectives: Various genomic alterations and genomic signatures, including amplification, mutations in , , and , and tumor mutational burden (TMB), have become important biomarkers for treatment selection in breast cancer (BC). This study aimed to investigate the mutational features of Chinese early-stage BC patients.

Methods: Tumors and matched blood samples collected from 589 Chinese patients with early-stage BC were sequenced using a commercial gene panel consisting of 520 cancer-related genes to analyze all types of genomic alterations and estimate the TMB status.

Results: A total of 18 genes were found to be more frequently mutated (<0.05) or amplified (<0.05) in stage T3-4 tumors as compared with T1-2 tumors. A total of 18 genes were found to be differentially mutated (<0.05) or amplified (<0.05) in patients with lymph node metastasis than those without lymph node metastasis. Younger patients (≤35 years) were more frequently identified with mutations or gene amplifications in eleven genes (<0.05). TMB >10mutations/Mb were found in 5.7% of our cohort. Although the TMB was similar for various molecular subtypes between our cohort and the BC cohort of The Cancer Genome Atlas (TCGA) study, the TMB were statistically different for HR+/HER-, HR+/HER2+, and triple-negative subtypes between our cohort and African Americans in the TCGA study. As compared to the TCGA BC cohort, our cohort had a much earlier median age of diagnosis (48 vs. 58 years, <0.001), and had significantly lower frequency of triple-negative subtype (11.5% vs. 18.4%, <0.001) and invasive lobular BC (2.4% vs. 19.0%, <0.001). Further subgroup analyses revealed that mutation rates in various genes including , , and were distinct for patients who were younger (≤35 years), had triple-negative or invasive lobular BC in our cohort than in the TCGA cohort.

Conclusions: This study revealed distinct mutational features of various molecular subtypes of early-stage BC among Chinese patients. Moreover, we provide new insights into the differences in early-stage BC between the East and West.
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http://dx.doi.org/10.3389/fonc.2021.618767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096980PMC
April 2021

High Performance Aqueous Li-Ion Flow Capacitor Realized Through Microstructure Design of Suspension Electrode.

Front Chem 2021 20;9:673179. Epub 2021 Apr 20.

Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, School of Materials Science and Technology, China University of Geosciences, Beijing, China.

Suspension electrode is the core of flowable electrochemical energy storage systems, which are considered suitable for large-scale energy storage. Nevertheless, obtaining suspension electrodes with both low viscosity and high conductivity is still a big challenge. In present work, spinel LiMnO was chosen as an example to make suspension with low viscosity and high conductivity through microstructure morphology control of solid particles and the contact mode between active materials and conductive additives in suspension electrode. By coating a thin layer of polyaniline on the surface of spherical spinel LiMnO, the resulting suspension showed much higher electronic conductivity (about 10 times) and lower viscosity (about 4.5 times) as compared to irregular and bare spinel LiMnO-based suspension counterpart. As a result, the Li-ion flow capacitor based on LiMnO and activated carbon suspensions exhibited a record energy density of 27.4 W h L at a power density of 22.5 W L under static condition to date, and can be smoothly work under an intermittent-flow mode. The strategy reported in this work is an effective way for obtaining suspension electrodes with low viscosity and high electronic conductivity simultaneously. It can not only be used in the flow capacitors, but also can be extended to other flowable electrochemical energy storage systems.
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http://dx.doi.org/10.3389/fchem.2021.673179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093800PMC
April 2021

Facile Synthesis of Imidazolium-Based Block Copolypeptides with Excellent Antimicrobial Activity.

Biomacromolecules 2021 May 6. Epub 2021 May 6.

Key Laboratory of Polymeric Materials and Application Technology of Hunan Province, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.

Antimicrobial polypeptides are promising mimics of antimicrobial peptides (AMPs) with low risks of antimicrobial resistance (AMR). Polypeptides with facile and efficient production, high antimicrobial activity, and low toxicity toward mammalian cells are highly desirable for practical applications. Herein, triblock copolypeptides with chloro groups (PPG-PCPBLG) and different main-chain lengths were synthesized via an ultrafast ring-opening polymerization (ROP) using a macroinitiator, namely poly(propylene glycol) bis(2-aminopropyl ether), and purified or nonpurified monomer (i.e., CPBLG-NCA). PPG-PCPBLG with 90 amino acid residues can be readily prepared within 300 s. Imidazolium-based block copolypeptides (PPG-PIL) were facilely prepared via nucleophilic substitution of PPG-PCPBLG with NaN and subsequent "click" chemistry. α-Helical PPG-PIL can self-assemble into nanostructured and cationic micelles which displayed highly potent antimicrobial activity and low hemolysis. The top-performing material, namely PPG-PIL, showed low minimum inhibitory concentration (MIC) against both Gram-positive and Gram-negative (25 μg mL). It also displayed low toxicity against mouse embryonic fibroblast (NIH 3T3) and human embryonic kidney (293T) cells at 2× MIC.
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http://dx.doi.org/10.1021/acs.biomac.1c00126DOI Listing
May 2021

Electroacupuncture at GV20‑GB7 regulates mitophagy to protect against neurological deficits following intracerebral hemorrhage via inhibition of apoptosis.

Mol Med Rep 2021 Jul 6;24(1). Epub 2021 May 6.

Department of Clinical Medicine, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China.

The acupuncture penetrating line of Baihui (GV20) to Qubin (GB7) spans the parietal, frontal and temporal lobes. The present study aimed to elucidate the mechanism by which electroacupuncture (EA) at GV20‑GB7 regulates mitophagy in intracerebral hemorrhage (ICH) and whether it serves a neuroprotective role. A whole blood‑induced ICH model was used. Mitophagy‑regulating proteins, including BCL/adenovirus E1B 19 kDa‑interacting protein 3 (BNIP3), PTEN‑induced putative kinase 1 (PINK1), Parkin and apoptosis‑associated proteins were detected by western blotting; autophagy following ICH was evaluated by immunofluorescent techniques; morphological characteristics of mitophagy were observed using transmission electron microscopy; and TUNEL assay was performed to determine the number of apoptotic cells. Immunohistochemistry was used to detect p53 expression. The protective role of EA (GV20‑GB7) via enhanced mitophagy and suppressed apoptosis in ICH was further confirmed by decreased modified neurological severity score. The results showed that EA (GV20‑GB7) treatment upregulated mitochondrial autophagy following ICH and inhibited apoptotic cell death. The mechanism underlying EA (GV20‑GB7) treatment may involve inhibition of p53, an overlapping protein of autophagy and apoptosis. EA (GV20‑GB7) treatment decreased neurobehavioral deficits following ICH but pretreatment with 3‑methyladenine counteracted the beneficial effects of EA (GV20‑GB7) treatment. In conclusion, EA (GV20‑GB7) improved recovery from ICH by regulating the balance between mitophagy and apoptosis.
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http://dx.doi.org/10.3892/mmr.2021.12131DOI Listing
July 2021

The Poor Prognosis of Patients with Stage III Gastric Cancer after D2 Dissection Is Mainly due to Lymphatic Metastasis, Especially the Metastasis of No.12a LN: A Nested Case-Control Study.

Oncol Res Treat 2021 May 4:1-7. Epub 2021 May 4.

Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: The prognosis of stage III gastric cancer (GC) patients based on the 8th edition TNM staging system after D2 dissection was still heterogeneous. We aimed to explore clinicopathological reasons that led to the poor prognosis of these patients, especially from a surgical aspect.

Methods: We divided 320 stage III GC patients who underwent distal or total gastrectomy with D2 lymphadenectomy into group 1 and group 2, according to the disease-free survival (DFS), and compared the clinicopathological features between these 2 groups. Then, we divided group 1 into group 1D and group 1T and group 2 into group 2D and group 2T, according to distal or total gastrectomy. Finally, we compared the status of lymph node (LN) metastasis in each group of perigastric LN between the subgroups, respectively.

Results: Univariate analyses revealed that patients' LN metastasis was the only significant difference between group 1 and group 2 (p < 0.05). Compared with group 1D, the percentage of patients who had metastatic LN in all groups of the perigastric LN (included No.1, 3, 4sb, 4d, 5, 6, 7, 8a, 9, 11p and 12a) increased in group 2D. A similar tendency was found in group 2T (included No.1, 2, 3, 4sa, 4sb, 4d, 5, 6, 7, 8a, 9, 10, 11p, 11d and 12a) compared with group 1T. Further multivariate regression analyses revealed that the increases of group No.12a and 5 LN were significant (p < 0.05) in group 2D, the increases of group No.12a, 8a and 6 LN were significant (p < 0.05) in group 2T, respectively. Among these, the increases of group No.12a LNs were the most significant (p < 0.01).

Conclusions: The poor prognosis of patients with stage III GC after D2 dissection is mainly due to lymphatic metastasis. The status of LN metastasis in the prognostic value of GC needs to be further enhanced in present staging systems. Maybe the metastasis of No.12a LN is the most significant poor prognostic factor of these patients. It points out that the dissection of No.12a LN should be carefully performed in radical gastrectomy.
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http://dx.doi.org/10.1159/000512934DOI Listing
May 2021

A four-hypoxia-genes-based prognostic signature for oral squamous cell carcinoma.

BMC Oral Health 2021 May 3;21(1):232. Epub 2021 May 3.

Department of Oral and Maxillofacial Surgery, Tianjin Stomatology Hospital, School of Medicine, NanKai University, Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, No. 75, Dagu North Road, Heping District, Tianjin, 300041, China.

Background: Oral squamous cell carcinoma (OSCC) is one of the most common maligancies of the head and neck. The prognosis was is significantly different among OSCC patients. This study aims to identify new biomarkers to establish a prognostic model to predict the survival of OSCC patients.

Methods: The mRNA expression and corresponding clinical information of OSCC patients were downloaded from The Cancer Genome Atlas and Gene Expression Omnibus. Additionally, a total of 26 hypoxia-related genes were also obtained from a previous study. Univariate Cox regression analysis and LASSO Cox regression analysis were performed to screen the optimal hypoxia-related genes which were associated with the prognosis of OSCC. to establish the predictive model (Risk Score) was established for estimating the patient's overall survival (OS). Multivariate Cox regression analysis was used to determine whether the Risk Score was an independent prognostic factor. Based on all the independent prognostic factors, nomogram was established to predict the OS probability of OSCC patients. The relative proportion of 22 immune cell types in each patient was evaluated by CIBERSORT software.

Results: We determined that a total of four hypoxia-related genes including ALDOA, P4HA1, PGK1 and VEGFA were significantly associated with the prognosis of OSCC patients. The nomogram established based on all the independent factors could reliably predict the long-term OS of OSCC patients. In addition, our resluts indicated that the inferior prognosis of OSCC patients with high Risk Score might be related to the immunosuppressive microenvironments.

Conclusion: This study shows that high expression of hypoxia-related genes including ALDOA, P4HA1, PGK1 and VEGFA is associated with poor prognosis in OSCC patients, and they can be used as potential markers for predicting prognosis in OSCC patients.
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http://dx.doi.org/10.1186/s12903-021-01587-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094530PMC
May 2021

Synthesis of cinnamoyl glucoside derivatives and their antiproliferation activities against murine melanoma B16-F10 cell line.

Carbohydr Res 2021 Apr 29;504:108332. Epub 2021 Apr 29.

Food and Pharmacy College, Xuchang University, Xuchang, China. Electronic address:

Twelve cinnamoyl glucoside derivatives were prepared by glycosylation of glucosyl trichloroacetimidate and cinnamic acid derivatives, followed by dechloroacetylation with a pyridine/HO mixture. Their structures were characterized by H and C NMR, as well as mass analysis. All the products were tested for their antiproliferation activities against murine melanoma B16-F10 cell line. Compounds 4e-4j were able to inhibit the proliferation of murine melanoma B16-F10 cell line with IC values of 17.38 ± 0.07, 9.87 ± 0.09, 9.69 ± 0.12, 29.42 ± 0.04, 32.95 ± 0.08, 25.68 ± 0.09 μM, respectively.
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http://dx.doi.org/10.1016/j.carres.2021.108332DOI Listing
April 2021

Guaiazulene Triggers ROS-Induced Apoptosis and Protective Autophagy in Non-small Cell Lung Cancer.

Front Pharmacol 2021 15;12:621181. Epub 2021 Apr 15.

Department of Oncology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Non-small cell lung cancer (NSCLC) is one of the most frequent cancers worldwide, yet effective treatment remains a clinical challenge. Guaiazulene (GYZ), a cosmetic color additive, has previously been characterized as a potential antitumor agent due to observed anticancer effects. However, the efficacy of GYZ in the treatment of NSCLC and the involved molecular mechanisms remain largely unknown. Here, we indicated a role for GYZ in the suppression of NSCLC both and via triggering reactive oxygen species (ROS)-induced apoptosis Concomitantly, GYZ induced complete autophagic flux in NSCLC cells via inhibiting the Akt/mTOR signaling pathway, which displayed cytoprotective effect against GYZ-induced growth suppression. Accompanied with autophagy inhibition obviously enhanced the effects of GYZ. Notably, GYZ acts synergistically with paclitaxel in the suppression of NSCLC . Together, our results for the first time reported that GYZ suppressed the proliferation of NSCLC and suggested a potential strategy for inhibiting NSCLC growth by combinational use of GYZ and autophagy inhibitors.
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http://dx.doi.org/10.3389/fphar.2021.621181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082441PMC
April 2021

Phase II multi-center trial of optical coherence tomography as an adjunct to white light cystoscopy for intravesical real time imaging and staging of bladder cancer.

Urol Oncol 2021 Apr 29. Epub 2021 Apr 29.

Scott Department of Urology, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX. Electronic address:

Background: Optical coherence tomography (OCT) is a novel imaging modality that provides microstructural information of different tissue layers using near-infrared light. This prospective, multicenter phase II trial aimed to assess the accuracy of OCT-assisted cystoscopy for bladder tumor staging.

Methods: Patients with primary or recurrent bladder tumors (Ta,T1) identified by outpatient cystoscopy were included. The primary objective was to assess the accuracy and positive predictive value of for determining tumor stage ≥T1 correlated by histopathology. 72 suspicious lesions from 63 patients were eligible to analyze in the study. All suspected lesions were evaluated with conventional cystoscopy, interpreted in real-time using OCT, and then resected. All results were compared to pathology. A total of 363 OCT images of tumor and normal mucosa in 25 patients were obtained to evaluate diagnostic efficacy of the computer-aided texture analysis algorithm.

Results: Sensitivity and specificity for predicting invasive tumors (≥ T1, n = 17) were 58.8% and 92.7% for cystoscopy, 64.7% and 100% for OCT-assisted cystoscopy, respectively. Accuracy of cystoscopy and OCT-assisted cystoscopy for predicting invasive tumor was 84.7% and 91.7% (P = 0.063), respectively. Cystoscopy and OCT-assisted cystoscopy correctly predicted T stage in 52/72 and 59/72 cases, respectively (P = 0.016). Cystoscopy missed 2 more invasive tumors than OCT-assisted cystoscopy. Cystoscopy (14.3%, 1/7) and OCT-assisted cystoscopy (28.6%, 2/7) showed relatively low sensitivity in detecting muscle invasion. Computer aided texture analysis demonstrated 75.1% sensitivity, 64.0% specificity, and 74.4% accuracy for differentiating tumor and normal urothelium.

Conclusion: OCT-assisted cystoscopy is a real time noninvasive and simple procedure that enhanced the accuracy of staging bladder tumors and prediction of any tumor invasion. Though the study did not meet the prespecified primary endpoint, OCT imaging is a promising adjunct to cystoscopy that may supplement intraoperative decision-making during transurethral resection of bladder tumors and additional prospective studies are warranted.
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http://dx.doi.org/10.1016/j.urolonc.2021.03.026DOI Listing
April 2021

Outcomes of tibial avulsion fracture of the posterior cruciate ligament treated with a homemade hook plate.

Injury 2021 Apr 20. Epub 2021 Apr 20.

Department of Traumatic Orthopedics, Wuxi Ninth People's Hospital affiliated to Soochow University, China. Electronic address:

Objective: To compare the clinical effects of an inverted L-shaped postero-medial approach with a homemade hook plate and arthroscopic fixation with Endobutton for tibial avulsion fractures of the posterior cruciate ligament.

Methods: The clinical data of 36 patients with PCL tibial avulsion fractures from January 2012 to December 2019 were analyzed retrospectively. The fractures were classified into Meyers-McKeever types II and III. Among them, 20 cases were treated with a homemade hook plate through an inverted L-shaped postero-medial approach (incision group), and 16 cases were treated with Endobutton under arthroscopy (arthroscopic group). The operative time, fracture union time, operative complications and range of motion of the knee joint were compared between the two groups. The stability of the knee joint was tested by the posterior drawer test, the functional recovery of the knee joint was evaluated by the Lysholm score, and the gastrocnemius muscle strength of the incision group was tested by performing heel raises with a single leg stance.

Results: There were no adverse events, such as fracture nonunion, infection, deep-vein thrombosis, abnormal hematoma or joint stiffness, in either group. The operative time was shorter in the incision group, and the difference was statistically significant (P < 0.05). There was no significant difference in fracture union time between the two groups (P > 0.05). At the last follow-up, there was no significant difference in range of motion or the Lysholm score between the two groups. There was no decrease in gastrocnemius muscle strength in the incision group.

Conclusions: The fixation of PCL tibial avulsion fractures with a homemade hook plate through an inverted L-shaped postero-medial approach is safe and effective. It showed almost the same satisfactory outcomes as arthroscopic Endobutton fixation.
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http://dx.doi.org/10.1016/j.injury.2021.04.042DOI Listing
April 2021

Controllable Spin Switching in a Single-Molecule Magnetic Tunneling Junction.

Nanoscale Res Lett 2021 May 1;16(1):77. Epub 2021 May 1.

School of Automotive Engineering, Changshu Institute of Technology, Changshu, China.

A new type of spin-current filter is proposed that consists of a single-molecule magnet (SMM) coupled to two normal metal electrodes. It is shown that this tunneling junction can generate a highly spin-polarized current, whose spin polarization can be switched by means of magnetic fields and gate voltages applied to the SMM. This spin switching in the SMM tunnel junction arises from spin-selective single-electron resonant tunneling via the lowest unoccupied molecular orbit of the SMM. The electron current spectrum is still spin polarized in the absence of an external magnetic field, which can help to judge whether the molecule's spin state has reached the ground-state doublet [Formula: see text]. This device can be realized with current technologies and may have practical use in spintronics and quantum information.
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http://dx.doi.org/10.1186/s11671-021-03531-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088424PMC
May 2021

Does Early Postoperative T1 Slope Change Affect Clinical Results of Patients With Single-level ACDF?

Clin Spine Surg 2021 Apr 28. Epub 2021 Apr 28.

Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.

Study Design: This was a retrospective study.

Objective: The main question of this study is whether the change of postoperative T1 slope will affect the clinical and imaging recovery of patients with single-level anterior cervical discectomy and fusion (ACDF).

Summary Of Background Data: The T1 slope after ACDF is different from that preoperatively, however, the clinical significance of this change has not been reported.

Materials And Methods: A retrospective analysis was conducted on 87 patients with single-level ACDF. Visual Analogue Scale was used to compare neck and upper limb pain before and after surgery. Neurological improvement was assessed by the Japanese Orthopaedic Association Scores and Neck Disability Index. Preoperative and postoperative T1 slope, occipital-C2 angle, C2-C7 overall curvature and functional spinal unit curvature were measured and analyzed by lateral cervical spine x-ray.

Results: All patients were followed up for 23.98±12.17 months. The Japanese Orthopaedic Association, Visual Analogue Scale, and Neck Disability Index scores as well as the overall curvature and change of C2-C7 and functional spinal unit were significantly improved in the last postoperative follow-up. At 12 months after surgery and the last follow-up, patients with increased T1 slope had more severe neck pain symptoms than those with decreased T1 slope (P<0.05). The overall curvature and change of C2-C7 in patients with increased T1 slope were better than those with decreased T1 slope (P<0.05).

Conclusion: For patients with increased postoperative T1 slope after single-level ACDF, the degree of postoperative neck pain was more severe, suggesting that some clinical intervention is needed.
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http://dx.doi.org/10.1097/BSD.0000000000001145DOI Listing
April 2021

Quantitative Proteomics Analysis Reveals the Function of the Putative Ester Cyclase UvEC1 in the Pathogenicity of the Rice False Smut Fungus .

Int J Mol Sci 2021 Apr 15;22(8). Epub 2021 Apr 15.

The Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.

Rice false smut is a fungal disease distributed worldwide and caused by . In this study, we identified a putative ester cyclase (named as UvEC1) as being significantly upregulated during infection. UvEC1 contained a SnoaL-like polyketide cyclase domain, but the functions of ketone cyclases such as SnoaL in plant fungal pathogens remain unclear. Deletion of caused defects in vegetative growth and conidiation. was also required for response to hyperosmotic and oxidative stresses and for maintenance of cell wall integrity. Importantly, Δ mutants exhibited reduced virulence. We performed a tandem mass tag (TMT)-based quantitative proteomic analysis to identify differentially accumulating proteins (DAPs) between the Δ mutant and the wild-type isolate HWD-2. Proteomics data revealed that UvEC1 has a variety of effects on metabolism, protein localization, catalytic activity, binding, toxin biosynthesis and the spliceosome. Taken together, our findings suggest that is critical for the development and virulence of .
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http://dx.doi.org/10.3390/ijms22084069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071170PMC
April 2021

Human-Induced Pluripotent Stem-Cell-Derived Smooth Muscle Cells Increase Angiogenesis to Treat Hindlimb Ischemia.

Cells 2021 Apr 2;10(4). Epub 2021 Apr 2.

Vascular Biology & Therapeutics Program, Yale School of Medicine, New Haven, CT 06519, USA.

Induced pluripotent stem cells (iPSC) represent an innovative, somatic cell-derived, easily obtained and renewable stem cell source without considerable ethical issues. iPSC and their derived cells may have enhanced therapeutic and translational potential compared with other stem cells. We previously showed that human iPSC-derived smooth muscle cells (hiPSC-SMC) promote angiogenesis and wound healing. Accordingly, we hypothesized that hiPSC-SMC may be a novel treatment for human patients with chronic limb-threatening ischemia who have no standard options for therapy. We determined the angiogenic potential of hiPSC-SMC in a murine hindlimb ischemia model. hiPSC-SMC were injected intramuscularly into nude mice after creation of hindlimb ischemia. Functional outcomes and perfusion were measured using standardized scores, laser Doppler imaging, microCT, histology and immunofluorescence. Functional outcomes and blood flow were improved in hiPSC-SMC-treated mice compared with controls (Tarlov score, < 0.05; Faber score, < 0.05; flow, = 0.054). hiPSC-SMC-treated mice showed fewer gastrocnemius fibers ( < 0.0001), increased fiber area ( < 0.0001), and enhanced capillary density ( < 0.01); microCT showed more arterioles (<96 μm). hiPSC-SMC treatment was associated with fewer numbers of macrophages, decreased numbers of M1-type ( < 0.05) and increased numbers of M2-type macrophages ( < 0.0001). Vascular endothelial growth factor (VEGF) expression in ischemic limbs was significantly elevated with hiPSC-SMC treatment ( < 0.05), and inhibition of VEGFR-2 with SU5416 was associated with fewer capillaries in hiPSC-SMC-treated limbs ( < 0.0001). hiPSC-SMC promote VEGF-mediated angiogenesis, leading to improved hindlimb ischemia. Stem cell therapy using iPSC-derived cells may represent a novel and potentially translatable therapy for limb-threatening ischemia.
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http://dx.doi.org/10.3390/cells10040792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066461PMC
April 2021

Based on biomedical index data: Risk prediction model for prostate cancer.

Medicine (Baltimore) 2021 Apr;100(17):e25602

Department of Pharmacy, Bengbu Medical College, Anhui Biochemical Drug Engineering Technology Research Center, Bengbu, China.

Abstract: To explore the influencing factors of prostate cancer occurrence, set up risk prediction model, require reference for the preliminary diagnosis of clinical doctors, this model searched database through the data of prostate cancer patients and prostate hyperplasia patients National Clinical Medical Science Data Center.With the help of Stata SE 12.0 and SPSS 25.0 software, the biases between groups were balanced by propensity score matching. Based on the matched data, the relevant factors were further screened by stepwise logistic regression analysis, the key variable and artificial neural network model are established. The prediction accuracy of the model is evaluated by combining the probability of test set with the area under receiver operating characteristic curve (ROC).After 1:2 PSM, 339 pairs were matched successfully. There are 159 cases in testing groups and 407 cases in training groups. And the regression model was P = 1 / (1 + e (0.122 ∗ age + 0.083 ∗ Apo lipoprotein C3 + 0.371 ∗ total prostate specific antigen (tPSA) -0.227 ∗ Apo lipoprotein C2-6.093 ∗ free calcium (iCa) + 0.428 ∗ Apo lipoprotein E-1.246 ∗ triglyceride-1.919 ∗ HDL cholesterol + 0.083 ∗ creatine kinase isoenzyme [CKMB])). The logistic regression model performed very well (ROC, 0.963; 95% confidence interval, 0.951 to 0.978) and artificial neural network model (ROC, 0.983; 95% confidence interval, 0.964 to 0.997). High degree of Apo lipoprotein E (Apo E) (Odds Ratio, [OR], 1.535) in blood test is a risk factor and high triglyceride (TG) (OR, 0.288) is a protective factor.It takes the biochemical examination of the case as variables to establish a risk prediction model, which can initially reflect the risk of prostate cancer and bring some references for diagnosis and treatment.
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http://dx.doi.org/10.1097/MD.0000000000025602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084031PMC
April 2021

Stabilizing LiNi0.8Co0.15Mn0.05O2 Cathode by Doping Sulfate for Lithium-ions Batteries.

ChemSusChem 2021 Apr 27. Epub 2021 Apr 27.

Western University, Advanced Materials for Clean Energy Group, CANADA.

Residual sulfate (SO 4 2- ) in precursor Ni 0.8 Co 0.15 Mn 0.05 (OH) 2 (pre-NCM) is commonly regarded as being harmful to NCM performance, which leads to significant performance losses, and also hampers the electrode fabrication. Therefore, manufacturers try their best to lower sulfate contents in pre-NCM. However, how the sulfate affects the cathode materials are not systematically studied. To address these issues, NCM is synthesized with different amount of intentionally added sulfate (NH 4 ) 2 SO 4 in pre-NCM. We demonstrate that anionic SO 4 2- doped in NCM can influence the grain size in sintering process and stabilize the layer structure during the charge-discharge process at a certain doping amount. The first-principles calculations suggest that the SO 4 2- doped in the TM layer can effectively facilitate Li + diffusion in the lattice. SO 4 2- doping could reduce the energy barrier for Li + migration, and then suppress the c axis drastically contract during cycling. The phase transition of H2-H3 caused by the c axis contract would be suppressed and the cycling performance is then enhanced. The capacity retention could reach 80.9% (0.2C) and 80.4% (1C) after 380 and 240 cycles in coin cells, respectively. These findings illustrate that a certain amount of sulfate could be benefit to NCM cathodes.
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http://dx.doi.org/10.1002/cssc.202100595DOI Listing
April 2021

Response to a letter to editor regarding "incidence of bone loss after prestige-LP cervical disc arthroplasty: a single-center retrospective study of 396 cases".

Spine J 2021 Apr;21(4):722-723

Department of Orthopedic Surgery, West China Hospital, Sichuan University, No. 37 Guo Xue Rd, Chengdu, 610041, China. Electronic address:

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http://dx.doi.org/10.1016/j.spinee.2020.12.006DOI Listing
April 2021

BMP4-mediated browning of perivascular adipose tissue governs an anti-inflammatory program and prevents atherosclerosis.

Redox Biol 2021 Apr 17;43:101979. Epub 2021 Apr 17.

Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology of School of Basic Medical Sciences and Department of Endocrinology and Metabolism of Zhongshan Hospital, Fudan University, Shanghai 200032, China. Electronic address:

Loss of perivascular adipose tissue (PVAT) impairs endothelial function and enhances atherosclerosis. However, the roles of PVAT thermoregulation in vascular inflammation and the development of atherosclerosis remains unclear. Bone morphogenetic protein 4 (BMP4) transforms white adipocyte to beige adipocyte, while promotes a brown-to-white shift in inter-scapular brown adipose tissue (BAT). Here, we found that knockdown of BMP4 in PVAT reduced expression of brown adipocyte-characteristic genes and increased endothelial inflammation in vitro co-culture system. Ablating BMP4 expression either in adipose tissues or specifically in BAT in ApoE mice demonstrated a marked exacerbation of atherosclerotic plaque formation in vivo. We further demonstrated that proinflammatory factors (especially IL-1β) increased in the supernatant of BMP4 knockdown adipocytes. Overexpression of BMP4 in adipose tissues promotes browning of PVAT and protects against atherosclerosis in ApoE mice. These findings uncover an organ crosstalk between PVAT and blood endothelial cells that is engaged in atherosclerosis.
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http://dx.doi.org/10.1016/j.redox.2021.101979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099561PMC
April 2021

9-Methylfascaplysin exerts anti-ischemic stroke neuroprotective effects via the inhibition of neuroinflammation and oxidative stress in rats.

Int Immunopharmacol 2021 Apr 23;97:107656. Epub 2021 Apr 23.

Translational Medicine Center of Pain, Emotion and Cognition, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo 315211, China; Ningbo Kangning Hospital, Ningbo 315020, China. Electronic address:

Objectives: This study was aimed to investigate the neuroprotective effects of 9-methylfascaplysin, a novel marine derivative derived from sponge, against middle cerebral artery occlusion/reperfusion (MCAO)-induced motor impairments, neuroinflammation and oxidative stress in rats.

Methods: Neurological and behavioral tests were used to evaluate behavioral changes. The 2, 3, 5-triphenyltetrazolium chloride staining was used to determine infarct size and edema extent. Activated microglia/macrophage was analyzed by immunohistochemical staining of Iba-1. RT-PCR and ELISA were used to measure the expression of inducible nitric oxide synthase, tumor necrosis factor-α, interleukin-1β, CD16 and CD206. Western blotting analysis was performed to explore the activation of nuclear factor-κB (NF-κB) and NLRP3. The levels of oxidative stress were studied by evaluating the activities of superoxide dismutase, catalase and glutathione peroxidase.

Results: Post-occlusion intracerebroventricular injection of 9-methylfascaplysin significantly attenuated motor impairments and infarct size in MCAO rats. Moreover, 9-methylfascaplysin reduced the activation of microglia/macrophage in ischemic penumbra as evidenced by the decreased Iba-1-positive area and the reduced expression of pro-inflammatory factors. Furthermore, 9-methylfascaplysin inhibited MCAO-induced oxidative stress and activation of NF-κB and NLRP3 inflammasome.

Conclusion: All the results suggested that 9-methylfascaplysin might produce neuroprotective effects against MCAO via the reduction of oxidative stress and neuroinflammation, simultaneously, possibly via the inhibition of NF-κB and NLRP3 inflammasome.
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http://dx.doi.org/10.1016/j.intimp.2021.107656DOI Listing
April 2021

Intraprocedural arrhythmia termination as an end point for hybrid ablation in patients with long-standing persistent atrial fibrillation: a 2-year follow-up study.

Interact Cardiovasc Thorac Surg 2021 Apr 23. Epub 2021 Apr 23.

Department of Cardiology, Hebei Yanda Hospital, Hebei, Hebei Province, China.

Objectives: Hybrid catheter and surgical ablation has emerged as an effective therapy for patients with persistent atrial fibrillation (AF). The aims of this study were to evaluate the relationship between intraprocedural arrhythmia termination and the long-term outcomes of hybrid ablation in patients with long-standing persistent AF.

Methods: From May 2015 through April 2019, 50 patients with persistent AF with a mean duration of 73.3 ± 62.1 (median 54) months underwent single-step hybrid ablation. Pulmonary vein isolation, left atrial posterior wall isolation and left atrial appendage excision or closure were performed through a left-sided thoracoscopic approach. Subsequently, all patients underwent high-density endocardial mapping and electrogram-based ablation with the end point of AF termination.

Results: We achieved intraprocedural AF termination in 84% (42/50) patients; this end point was reached in 16 patients during surgical ablation and in 26 patients during catheter ablation. Seven patients underwent repeat catheter ablation. After a mean follow-up period of 29 ± 13 months, the freedom from atrial tachyarrhythmia of a single procedure without the use of antiarrhythmic drugs was 70% (35/50). In the Cox regression model, intraprocedural termination of AF (hazard ratio 0.205, 95% confidence interval 0.058-0.730; P = 0.014) was the sole predictor of success.

Conclusions: The 2-year outcomes of a one-stop hybrid ablation with an end point of AF termination are promising in patients with long-standing persistent AF.
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http://dx.doi.org/10.1093/icvts/ivab055DOI Listing
April 2021

Building an OMOP common data model-compliant annotated corpus for COVID-19 clinical trials.

J Biomed Inform 2021 Apr 28;118:103790. Epub 2021 Apr 28.

Department of Biomedical Informatics, Columbia University, New York, NY, USA. Electronic address:

Clinical trials are essential for generating reliable medical evidence, but often suffer from expensive and delayed patient recruitment because the unstructured eligibility criteria description prevents automatic query generation for eligibility screening. In response to the COVID-19 pandemic, many trials have been created but their information is not computable. We included 700 COVID-19 trials available at the point of study and developed a semi-automatic approach to generate an annotated corpus for COVID-19 clinical trial eligibility criteria called COVIC. A hierarchical annotation schema based on the OMOP Common Data Model was developed to accommodate four levels of annotation granularity: i.e., study cohort, eligibility criteria, named entity and standard concept. In COVIC, 39 trials with more than one study cohorts were identified and labelled with an identifier for each cohort. 1,943 criteria for non-clinical characteristics such as "informed consent", "exclusivity of participation" were annotated. 9767 criteria were represented by 18,161 entities in 8 domains, 7,743 attributes of 7 attribute types and 16,443 relationships of 11 relationship types. 17,171 entities were mapped to standard medical concepts and 1,009 attributes were normalized into computable representations. COVIC can serve as a corpus indexed by semantic tags for COVID-19 trial search and analytics, and a benchmark for machine learning based criteria extraction.
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http://dx.doi.org/10.1016/j.jbi.2021.103790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079156PMC
April 2021

A Framework for Automatic Burn Image Segmentation and Burn Depth Diagnosis Using Deep Learning.

Comput Math Methods Med 2021 7;2021:5514224. Epub 2021 Apr 7.

The People's Hospital of Jianggan District, Hangzhou, Zhejiang, China.

Burn is a common traumatic disease with high morbidity and mortality. The treatment of burns requires accurate and reliable diagnosis of burn wounds and burn depth, which can save lives in some cases. However, due to the complexity of burn wounds, the early diagnosis of burns lacks accuracy and difference. Therefore, we use deep learning technology to automate and standardize burn diagnosis to reduce human errors and improve burn diagnosis. First, the burn dataset with detailed burn area segmentation and burn depth labelling is created. Then, an end-to-end framework based on deep learning method for advanced burn area segmentation and burn depth diagnosis is proposed. The framework is firstly used to segment the burn area in the burn images. On this basis, the calculation of the percentage of the burn area in the total body surface area (TBSA) can be realized by extending the network output structure and the labels of the burn dataset. Then, the framework is used to segment multiple burn depth areas. Finally, the network achieves the best result with IOU of 0.8467 for the segmentation of burn and no burn area. And for multiple burn depth areas segmentation, the best average IOU is 0.5144.
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http://dx.doi.org/10.1155/2021/5514224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046560PMC
April 2021

Modulation the acidic and basic site concentration of the metal-organic framework derivatives to promote the carbon dioxide epoxidation reaction.

Chemistry 2021 Apr 19. Epub 2021 Apr 19.

Hunan University, College of Chemistry and Chemical Engineering, CHINA.

Metal-organic Framework (MOF) is an ideal precursor/template for porous carbon, and its active components are uniformly doped, which can be used in energy storage and catalytic conversion fields. Metal-organic framework PCN-224 with carboxylporphyrin as ligand was synthesized, and then Zn 2+ and Co 2+ ions were coordinated in the center of the porphyrin ring by post-modification. Here, PCN-224-ZnCo with different ratios of bimetallic Zn 2+ /Co 2+ ions is used as the precursor, and the metal-nitrogen-carbon(M-N-C) material of PCN-224-ZnCo-950 was obtained by pyrolysis the precursor at 950°C in Ar. Because Zn is easy to volatilize at 950°C, the formed M-N-C materials can reflect different Co contents and different basic site concentrations. The formed material still maintains the original basic framework. With the increase of Zn 2+ /Co 2+ ratio in precursor, the concentration of N-containing alkaline sites in pyrolysis products gradually increase. Compared with the precursor, PCN-224-ZnCo 1 -950 with Zn 2+ /Co 2+ =1:1 has greatly improved basicity and suitable acidic/ alkaline site concentration. It can be efficiently used to carbon dioxide absorption and catalyze the cycloaddition of CO 2 with epoxide. More importantly, the current method of adjusting the acidic/basic sites in M-N-C materials through volatilization of volatile metals can provide an effective strategy for adjusting the catalysis of MOF derivatives with porphyrin structure.
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http://dx.doi.org/10.1002/chem.202100430DOI Listing
April 2021

The FENDRR/FOXC2 Axis Contributes to Multidrug Resistance in Gastric Cancer and Correlates With Poor Prognosis.

Front Oncol 2021 22;11:634579. Epub 2021 Mar 22.

State Key Laboratory of Cancer Biology and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

The dysregulation of long non-coding RNAs (lncRNAs) and transcription factors (TFs) is closely related to the development and progression of drug resistance in cancer chemotherapy. However, their regulatory interactions in the multidrug resistance (MDR) of gastric cancer (GC) has largely remained unknown. In this study, we report a novel oncogenic role of lncRNA FENDRR in conferring MDR in GC by coordinated regulation of FOXC2 expression at the transcriptional and posttranscriptional levels. and experiments demonstrated that downregulation of FENDRR expression remarkably decreased drug resistant ability of GC MDR cells while upregulation of FENDRR expression produced the opposite effect. FENDRR overexpression was observed in MDR GC cell lines, patient-derived xenografts, and clinical samples. And the high levels of FENDRR expression were correlated with poor prognosis in GC patients. Regarding the mechanism, FENDRR was revealed to increase proto-oncogene FOXC2 transcription by performing an enhancer-like role in the nucleus and by sponging miR-4700-3p in the cytoplasm. Both FOXC2 and miR-4700-3p were shown to be functionally involved in the FENDRR-induced chemoresistance. In addition, there is a positive correlation between FENDRR and FOXC2 expression in clinic and the overexpressed FOXC2 indicated a poor prognosis in GC patients. Collectively, our findings provide a new perspective for the lncRNA-TF regulatory interaction involved in MDR, suggesting that targeting the FENDRR/FOXC2 axis may be an effective approach to circumvent GC chemoresistance.
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http://dx.doi.org/10.3389/fonc.2021.634579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044876PMC
March 2021

[Effect of modified lateral mass screws implantation strategy on axial symptoms in cervical expansive open-door laminoplasty].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Apr;35(4):445-452

Department of Orthopedics, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P.R.China.

Objective: To investigate the effect of modified lateral mass screws implantation strategy on axial symptoms in cervical expansive open-door laminoplasty.

Methods: A clinical data of 166 patients, who underwent cervical expansive open-door laminoplasty between August 2011 and July 2016 and met the selection criteria, was retrospective analyzed. Among them, 81 patients were admitted before August 2014 using the traditional mini-plate placement and lateral mass screws implantation strategy (control group), and 85 patients were admitted after August 2014 using modified lateral mass screws implantation strategy (modified group). There was no significant difference in the gender composition, age, clinical diagnosis, disease duration, diseased segment, and preoperative Japanese Orthopaedic Association (JOA) score, pain visual analogue scale (VAS) score, Neck Disability Index (NDI), cervical curvature and range of motion, spinal canal diameter and cross-sectional areas, and Pavlov's value between the two groups ( >0.05). The operation time, intraoperative blood loss, the number of facet joints penetrated by lateral mass screws, effectiveness evaluation indexes (JOA score and improvement rate, VAS score, NDI), imaging evaluation indexes (cervical curvature and range of motion, spinal canal diameter and cross-sectional areas, Pavlov's value, and lamina open angle), and complications were recorded and compared between the two groups.

Results: The modified group had shorter operation time and lower intraoperative blood loss than the control group ( <0.05). There were 121 (29.9%, 121/405) and 10 (2.4%, 10/417) facet joints penetrated by lateral mass screws in control and modified groups, respectively; and the difference in incidence was significant ( =115.797, =0.000). Eighteen patients in control group had 3 or more facet joints penetrated while no patients in modified group suffered 3 or more facet joint penetrated. The difference between the two groups was significant ( =0.000). All patients were followed up, the follow-up time was (28.7±4.9) months in modified group and (42.4±10.7) months in control group, showing significant difference ( =10.718, =0.000). The JOA score, VAS score, and NDI at last follow-up of the two groups were significantly improved compared with preoperative ( <0.05); there was no significant difference in JOA score and improvement rate and VAS score between the two groups ( >0.05), but the NDI was significantly lower in modified group than in control group ( <0.05). There were significant differences in cervical curvature and range of motion, spinal canal diameter, Pavlov's value, and cross-sectional areas at last follow-up when compared with those before operation in both groups ( <0.05). There was no significant difference in the above indicators and lamina open angle between the two groups ( >0.05). The modified group has a relative lower axial symptom rate (23/85, 27.1%) than the control group (27/81, 33.3%), but the difference was not significant ( =-1.446, =0.148). There was no significant differences between the two groups in the incidences of C nerve root palsy, cerebrospinal fluid leakage, wound infection, and lung or urinary tract infection ( >0.05).

Conclusion: In the cervical expansive open-door laminoplasty, the modified lateral mass screws implantation strategy can effectively reduce the risk of lateral mass screw penetrated to the cervical facet joints, and thus has a positive significance in avoiding the axial symptoms caused by facet joint destruction.
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http://dx.doi.org/10.7507/1002-1892.202010024DOI Listing
April 2021

Self-contained Janus Aerogel with Antifouling and Salt-Rejecting Properties for Stable Solar Evaporation.

ACS Appl Mater Interfaces 2021 Apr 14;13(16):18829-18837. Epub 2021 Apr 14.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Jiangsu Key Laboratory of Fine Chemicals and Functional Polymer Materials, Nanjing Tech University, No. 5 Xin Mofan Road, Nanjing 210009, China.

Janus structural interfacial vaporization by separating the solar absorber from the bulk working fluid has attracted tremendous attention due to its low heat losses and high solar conversion efficiency for desalination, water purification, energy generation, etc. However, a totally separated double-deck structure with a discontinuous interfacial transition and inefficient photothermic materials undermines its long-term use and large-scale practical exploitation. Herein, a low-cost Janus monolithic chitosan aerogel with continuous aligned run-through microchannels has been demonstrated to have a highly efficient photothermic effect on seawater desalination and wastewater purification. The top solar absorber layer enhances broadband light absorption and photothermal conversion efficiency via the multiple internal reflection of incident light in the aligned microchannels. Moreover, the insulating/hydrophilic bottom layer promotes water transportation and heat localization, and simultaneously prevents salt/fouling accumulation. As a result, a long-term solar vaporization rate of ∼1.76 kg m h is achieved, corresponding to a light-to-vapor efficiency of ∼91% under 1 sun irradiation. Notably, the large-vessel microchannels throughout the aerogel and favorable swelling property provide sufficient water replenishment and storage for completely isolating self-contained evaporation, illustrating an enhanced and time-extended self-contained solar steam generation. Such a low-cost bilayer aerogel with remarkable cycling stability in various fluids offers potential opportunities for freshwater production in remote rural areas.
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http://dx.doi.org/10.1021/acsami.1c02198DOI Listing
April 2021