Publications by authors named "Hao Liu"

3,142 Publications

  • Page 1 of 1

DeepDRiD: Diabetic Retinopathy-Grading and Image Quality Estimation Challenge.

Patterns (N Y) 2022 Jun 20;3(6):100512. Epub 2022 May 20.

Department of Computer Science and Engineering, The Ohio State University, Ohio, USA.

We described a challenge named "Diabetic Retinopathy (DR)-Grading and Image Quality Estimation Challenge" in conjunction with ISBI 2020 to hold three sub-challenges and develop deep learning models for DR image assessment and grading. The scientific community responded positively to the challenge, with 34 submissions from 574 registrations. In the challenge, we provided the DeepDRiD dataset containing 2,000 regular DR images (500 patients) and 256 ultra-widefield images (128 patients), both having DR quality and grading annotations. We discussed details of the top 3 algorithms in each sub-challenges. The weighted kappa for DR grading ranged from 0.93 to 0.82, and the accuracy for image quality evaluation ranged from 0.70 to 0.65. The results showed that image quality assessment can be used as a further target for exploration. We also have released the DeepDRiD dataset on GitHub to help develop automatic systems and improve human judgment in DR screening and diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.patter.2022.100512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214346PMC
June 2022

Diagnostic accuracy and potential covariates of artificial intelligence for diagnosing orthopedic fractures: a systematic literature review and meta-analysis.

Eur Radiol 2022 Jun 27. Epub 2022 Jun 27.

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, No. 37 Guo Xue Rd, Chengdu, 610041, China.

Objectives: To systematically quantify the diagnostic accuracy and identify potential covariates affecting the performance of artificial intelligence (AI) in diagnosing orthopedic fractures.

Methods: PubMed, Embase, Web of Science, and Cochrane Library were systematically searched for studies on AI applications in diagnosing orthopedic fractures from inception to September 29, 2021. Pooled sensitivity and specificity and the area under the receiver operating characteristic curves (AUC) were obtained. This study was registered in the PROSPERO database prior to initiation (CRD 42021254618).

Results: Thirty-nine were eligible for quantitative analysis. The overall pooled AUC, sensitivity, and specificity were 0.96 (95% CI 0.94-0.98), 90% (95% CI 87-92%), and 92% (95% CI 90-94%), respectively. In subgroup analyses, multicenter designed studies yielded higher sensitivity (92% vs. 88%) and specificity (94% vs. 91%) than single-center studies. AI demonstrated higher sensitivity with transfer learning (with vs. without: 92% vs. 87%) or data augmentation (with vs. without: 92% vs. 87%), compared to those without. Utilizing plain X-rays as input images for AI achieved results comparable to CT (AUC 0.96 vs. 0.96). Moreover, AI achieved comparable results to humans (AUC 0.97 vs. 0.97) and better results than non-expert human readers (AUC 0.98 vs. 0.96; sensitivity 95% vs. 88%).

Conclusions: AI demonstrated high accuracy in diagnosing orthopedic fractures from medical images. Larger-scale studies with higher design quality are needed to validate our findings.

Key Points: • Multicenter study design, application of transfer learning, and data augmentation are closely related to improving the performance of artificial intelligence models in diagnosing orthopedic fractures. • Utilizing plain X-rays as input images for AI to diagnose fractures achieved results comparable to CT (AUC 0.96 vs. 0.96). • AI achieved comparable results to humans (AUC 0.97 vs. 0.97) but was superior to non-expert human readers (AUC 0.98 vs. 0.96, sensitivity 95% vs. 88%) in diagnosing fractures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00330-022-08956-4DOI Listing
June 2022

Imputation of the major histocompatibility complex region identifies major independent variants associated with bullous pemphigoid and dermatomyositis in Han Chinese.

J Dermatol 2022 Jun 25. Epub 2022 Jun 25.

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

As autoimmune skin diseases, both bullous pemphigoid (BP) and dermatomyositis (DM) show significant associations with the major histocompatibility complex (MHC) region. In fact, the coexistence of BP and DM has been previously reported. Therefore, we hypothesized that there may be a potential genetic correlation between BP and DM. Based on data for 312 BP patients, 128 DM patients, and 6793 healthy control subjects, in the MHC region, we imputed single-nucleotide polymorphisms (SNP), insertions and deletions (INDEL), and copy number variations (CNV) using the 1KGP phase 3 dataset and amino acids (AA) and SNP using a Han-MHC reference database. An association study revealed the most significant SNP associated with BP, namely, rs580921 (p = 1.06E-08, odds ratio [OR] = 1.61, 95% confidence interval [CI] = 1.37-1.90), which is located in the C6orf10 gene, and the most significant classic human leukocyte antigen (HLA) allele associated with DM, namely, HLA-DPB1*1701 (p = 6.56E-10, OR = 3.61, 95% CI = 2.40-5.42). Further stepwise regression analyses with rs580921 identified a threonine at position 163 of the HLA-B gene as a new independent disease-associated AA, and HLA-DPB1*1701 indicated that no loci were significant. Three-dimensional ribbon models revealed that the HLA-B AA position 163 (p = 3.93E-07, OR = 1.64, 95% CI = 1.35-1.98) located in the α2 domain of the HLA-B molecule was involved in the process of specific antigen presentation. The calculations showed that there was no significant genetic correlation between BP and DM. Our study identified three significant loci in the MHC region, proving that the HLA region was significantly correlated with BP and DM separately. Our research highlights the key role of the MHC region in disease susceptibility.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1346-8138.16499DOI Listing
June 2022

Quality of life in patients with cancer-related Brown IIb maxillary defect: A comparison between conventional obturation rehabilitation and submental flap reconstruction.

Oral Oncol 2022 Jun 21;132:105980. Epub 2022 Jun 21.

Department of Oromaxillofacial-Head and Neck Surgery, Tianjin Stomatological Hospital, 75 Dagu Road, Heping District, Tianjin 300041, PR China; Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, 75 Dagu Road, Heping District, Tianjin 300041, PR China. Electronic address:

Objectives: The purpose of this retrospective study was to compare the differences in quality of life (QOL) outcomes between the conventional obturator prostheses (COP) and the pedicled submental artery island flap (SAIF) in the reconstruction of Brown IIb maxillary defects.

Materials And Methods: The QOL of 116 eligible patients who had a lapse ≥ 12 months after the cancer-related maxilla ablation was evaluated by the University of Washington quality of life scale (UW-QOL), Performance Status Scale for Head and Neck (PSS-HN), and Obturator Functioning Scale (OFS).

Results: Patients in the SAIF group reported statistically and clinically significant higher overall QOL scores but lower chewing scores in the UW-QOL scale when compared with those in the COP group (P < 0.05). Clinically significantly higher scores were also observed in the recreation and anxiety domains in the UW-QOL scale for the SAIF group, but there was no statistical significances. The COP group reported more complaints about the nasal leakage when swallowing and the shape of the upper lip, and had a stronger willingness to avoid family or social events in the OFS (P < 0.05).

Conclusions: For patients with Brown IIb defects, SAIF reconstruction can achieve reduced nasal leakage when swallowing, improved upper-lip contour, increased social activity, and superior overall QOL than COP. The inferior chewing function in the SAIF group indicated the need for dental rehabilitation with a conventional denture or osseointegrated implants.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.oraloncology.2022.105980DOI Listing
June 2022

Single-Atom Ce-Modified α-FeO for Selective Catalytic Reduction of NO with NH.

Environ Sci Technol 2022 Jun 24. Epub 2022 Jun 24.

Jiangsu Key Laboratory of Vehicle Emissions Control, School of the Environment, Nanjing University, Nanjing 210093, P. R. China.

A single-atom Ce-modified α-FeO catalyst (FeCeO catalyst with 7% atomic percentage of Ce) was synthesized by a citric acid-assisted sol-gel method, which exhibited excellent performance for selective catalytic reduction of NO with NH (NH-SCR) over a wide operating temperature window. Remarkably, it maintained ∼93% NO conversion efficiency for 168 h in the presence of 200 ppm SO and 5 vol % HO at 250 °C. The structural characterizations suggested that the introduction of Ce leads to the generation of local Fe-O-Ce sites in the FeO matrix. Furthermore, it is critical to maintain the atomic dispersion of the Ce species to maximize the amounts of Fe-O-Ce sites in the Ce-doped FeO catalyst. The formation of CeO nanoparticles due to a high doping amount of Ce species leads to a decline in catalytic performance, indicating a size-dependent catalytic behavior. Density functional theory (DFT) calculation results indicate that the formation of oxygen vacancies in the Fe-O-Ce sites is more favorable than that in the Fe-O-Fe sites in the Ce-free α-FeO catalyst. The Fe-O-Ce sites can promote the oxidation of NO to NO on the FeCeO catalyst and further facilitate the reduction of NO by NH. In addition, the decomposition of NHHSO can occur at lower temperatures on the FeCeO catalyst containing atomically dispersed Ce species than on the α-FeO reference catalyst, resulting in the good SO/HO resistance ability in the NH-SCR reaction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.2c02916DOI Listing
June 2022

Efficacy of Topical Intervention for Recurrent Aphthous Stomatitis: A Network Meta-Analysis.

Medicina (Kaunas) 2022 Jun 7;58(6). Epub 2022 Jun 7.

The Center of Experimental Teaching Management, Chongqing Medical University, Chongqing 400016, China.

To compare the efficacy and safety of topical interventions used for recurrent aphthous stomatitis. This network meta-analysis was conducted in accordance with the PRISMA statement. We searched four electronic databases, PubMed, Web of Science (WOS), Cochrane Central Register of Controlled Trials and Embase, for randomized controlled trials reporting efficacy and safety data on topical interventions for recurrent aphthous stomatitis. We performed a quality evaluation using a methodology based on the Cochrane Handbook. Two authors independently extracted data on healing effect, size reduction effect, symptom reduction effect, recurrence and safety assessment. Network meta-analysis was then performed using ADDIS and RevMan. : A total of 72 trials (5272 subjects) involving 29 topical interventions were included. Honey, lnsulin liposome gel, laser, amlexanox, glycyrrhiza and triamcinolone had better efficacy performance. Probiotics and chlorhexidine helped to prolong ulcer intervals and reduce recurrence. Doxycycline and penicillin had a high risk of adverse events. Hematologic evaluation showed no preference. The rank possibility of size-reducing effect and symptom-reducing effect supported the short-term effect of laser and the long-term effect of probiotics. : We recommend the use of laser as a short-term intervention during the exacerbation phase of RAS and probiotics as a long-term intervention during the exacerbation and remission phases of RAS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/medicina58060771DOI Listing
June 2022

Genomic Variation-Mediating Fluconazole Resistance in Yeast.

Biomolecules 2022 Jun 17;12(6). Epub 2022 Jun 17.

Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Fungal infections pose a serious and growing threat to public health. These infections can be treated with antifungal drugs by killing hazardous fungi in the body. However, the resistance can develop over time when fungi are exposed to antifungal drugs by generating genomic variations, including mutation, aneuploidy, and loss of heterozygosity. The variations could reduce the binding affinity of a drug to its target or block the pathway through which drugs exert their activity. Here, we review genomic variation-mediating fluconazole resistance in the yeast , with the hope of highlighting the functional consequences of genomic variations for the antifungal resistance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/biom12060845DOI Listing
June 2022

Caspase-4/11 is critical for angiogenesis by repressing Notch1 signaling via inhibiting γ-secretase activity.

Br J Pharmacol 2022 Jun 23. Epub 2022 Jun 23.

Aab Cardiovascular Research Institute, Department of Medicine and Dentistry, University of Rochester, Rochester, NY, USA.

Background And Purpose: Notch1 activation mediated by γ-secretase is critical for angiogenesis. GeneCards database predicted that Caspase-4 (CASP4, with murine ortholog CASP11) interacts with presenilin-1, the catalytic core of γ-secretase. Therefore, we investigated the role of CASP4/11 in angiogenesis.

Experimental Approach: In vivo, we studied the role of Casp11 in several angiogenesis mouse models using Casp11 wild-type and knockout mice. In vitro, we detected the effects of CASP4 on endothelial functions and Notch signaling by depleting or overexpressing CASP4 in human umbilical vein endothelial cells (HUVECs). The functional domain responsible for the binding of CASP4 and presenilin-1 was detected by mutagenesis and co-immunoprecipitation.

Key Results: Casp11 deficiency remarkably impaired adult angiogenesis in ischemic hindlimbs, melanoma xenografts and Matrigel plugs, but not the developmental angiogenesis of retina. Bone marrow transplantation revealed that the pro-angiogenic effect depended on CASP11 derived from non-hematopoietic cells. CASP4 expression was induced by inflammatory factors and CASP4 knockdown decreased cell viability, proliferation, migration and tube formation in HUVECs. Mechanistically, CASP4/11 deficiency increased Notch1 activation in vivo and in vitro, while CASP4 overexpression repressed Notch1 signaling in HUVECs. Moreover, CASP4 knockdown increased γ-secretase activity. γ-Secretase inhibitor DAPT restored the effects of CASP4 siRNA on Notch1 activation and angiogenesis in HUVECs. Notably, the catalytic activity of CASP4/11 was dispensable. Instead, CASP4 directly interacted with presenilin-1 through the caspase recruitment domain (CARD).

Conclusions And Implications: These findings reveal a critical role of CASP4/11 in adult angiogenesis and make this molecule a promising therapeutic target for angiogenesis-related diseases in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/bph.15904DOI Listing
June 2022

Adaptive Dynamic Therapy and Survivorship for Operable Pancreatic Cancer.

JAMA Netw Open 2022 Jun 1;5(6):e2218355. Epub 2022 Jun 1.

Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania.

Importance: Neoadjuvant therapy is increasingly used in localized pancreatic carcinoma, and survival is correlated with carbohydrate antigen 19-9 (CA19-9) levels and histopathologic response following neoadjuvant therapy. With several regimens now available, the choice of chemotherapy could be best dictated by response to neoadjuvant therapy (as measured by CA19-9 levels and/or pathologic response), a strategy defined herein as adaptive dynamic therapy.

Objective: To evaluate the association of adaptive dynamic therapy with oncologic outcomes in patients with surgically resected pancreatic cancer.

Design, Setting, And Participants: This retrospective cohort study included patients with localized pancreatic cancer who were treated with either gemcitabine/nab-paclitaxel or fluorouracil, leucovorin, irinotecan, and oxaliplatin (FOLFIRINOX) preoperatively between 2010 and 2019 at a high-volume tertiary care academic center. Participants were identified from a prospectively maintained database and had a median follow-up of 49 months. Data were analyzed from October 17 to November 24, 2020.

Exposures: The adaptive dynamic therapy group included 219 patients who remained on or switched to an alternative regimen as dictated by CA19-9 response and for whom the adjuvant regimen was selected based on CA19-9 and/or pathologic response. The nonadaptive dynamic therapy group included 103 patients who had their chemotherapeutic regimen selected independent of CA19-9 and/or tumoral response.

Main Outcomes And Measures: Prognostic implications of dynamic perioperative therapy assessed through Kaplan-Meier analysis, Cox regression, and inverse probability weighted estimators.

Results: A total of 322 consecutive patients (mean [SD] age, 65.1 [9] years; 162 [50%] women) were identified. The adaptive dynamic therapy group, compared with the nonadaptive dynamic therapy group, had a more pronounced median (IQR) decrease in CA19-9 levels (-80% [-92% to -56%] vs -45% [-81% to -13%]; P < .001), higher incidence of complete or near-complete tumoral response (25 [12%] vs 2 [2%]; P = .007), and lower median (IQR) number of lymph node metastasis (1 [0 to 4] vs 2 [0 to 4]; P = .046). Overall survival was significantly improved in the dynamic group compared with the nondynamic group (38.7 months [95% CI, 34.0 to 46.7 months] vs 26.5 months [95% CI, 23.5 to 32.9 months]; P = .03), and on adjusted analysis, dynamic therapy was independently associated with improved survival (hazard ratio, 0.73; 95% CI, 0.53 to 0.99; P = .04). On inverse probability weighted analysis of 320 matched patients, the average treatment effect of dynamic therapy was to increase overall survival by 11.1 months (95% CI, 1.5 to 20.7 months; P = .02).

Conclusions And Relevance: In this cohort study that sought to evaluate the role of adaptive dynamic therapy in localized pancreatic cancer, selecting a chemotherapeutic regimen based on response to preoperative therapy was associated with improved survival. These findings support an individualized and in vivo assessment of response to perioperative therapy in pancreatic cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1001/jamanetworkopen.2022.18355DOI Listing
June 2022

Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review.

Gels 2022 Jun 15;8(6). Epub 2022 Jun 15.

Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, China.

Organoids are novel in vitro cell culture models that enable stem cells (including pluripotent stem cells and adult stem cells) to grow and undergo self-organization within a three-dimensional microenvironment during the process of differentiation into target tissues. Such miniature structures not only recapitulate the histological and genetic characteristics of organs in vivo, but also form tissues with the capacity for self-renewal and further differentiation. Recent advances in biomaterial technology, particularly hydrogels, have provided opportunities to improve organoid cultures; by closely integrating the mechanical and chemical properties of the extracellular matrix microenvironment, with novel synthetic materials and stem cell biology. This systematic review critically examines recent advances in various strategies and techniques utilized for stem-cell-derived organoid culture, with particular emphasis on the application potential of hydrogel technology in organoid culture. We hope this will give a better understanding of organoid cultures for modelling diseases and tissue engineering applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/gels8060379DOI Listing
June 2022

Bibliometric and visualized analysis of the top 100 most-cited articles on anterior cervical surgery.

EFORT Open Rev 2021 Dec 10;6(12):1203-1213. Epub 2021 Dec 10.

1Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu, China.

Anterior cervical surgery (ACS) owes its development to various pioneering individuals whose revolutionary works form key advances and guide current medical decisions. This bibliometric study aimed to identify, analyse and visualize the main features of the most-cited papers in ACS. The citation count for the top 100 most-cited articles ranged from 148 to 1,197, and citations per year ranged from 3.1 to 89.8. The articles were published from 1958 to 2016, with the 2000s being the most active decade. There was an inverse correlation between the average citations per year since publication and article age. The oldest as well as most-cited two articles were both published in 1958 by Smith and Robinson, and Cloward, respectively. In their studies, the authors individually described the technique of anterior cervical discectomy with fusion (ACDF). The most popular keywords were: 'fusion' (22), 'spine' (20), 'cervical spine' (16), 'complications' (15), 'arthrodesis' (13), 'interbody fusion' (13), 'bone morphogenetic protein' (13), and 'radiculopathy' (12). ACDF was the most frequent surgical procedure (80%), while cervical disc arthroplasty is of gradual greater impact. The surgical techniques of ACDF have remained unaltered for over 60 years. More attempts are needed to promote its development. Cite this article: EFORT Open Rev 2021;6:1203-1213. DOI: 10.1302/2058-5241.6.210074.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1302/2058-5241.6.210074DOI Listing
December 2021

Applications of Artificial Intelligence to Popularize Legal Knowledge and Publicize the Impact on Adolescents' Mental Health Status.

Authors:
Hao Liu

Front Psychiatry 2022 26;13:902456. Epub 2022 May 26.

School of Law, Chongqing University, Chongqing, China.

Artificial intelligence (AI) advancements have radically altered human production and daily living. When it comes to AI's quick rise, it facilitates the growth of China's citizens, and at the same moment, a lack of intelligence has led to several concerns regarding regulations and laws. Current investigations regarding AI on legal knowledge do not have consistent benefits in predicting adolescents' psychological status, performance, etc. The study's primary purpose is to examine the influence of AI on the legal profession and adolescent mental health using a novel cognitive fuzzy K-nearest neighbor (CF-KNN). Initially, the legal education datasets are gathered and are standardized in the pre-processing stage through the normalization technique to retrieve the unwanted noises or outliers. When normalized data are transformed into numerical features, they can be analyzed using a variational autoencoder (VAE) approach. Multi-gradient ant colony optimization (MG-ACO) is applied to select a proper subset of the features. Tree C4.5 (T-C4.5) and fitness-based logistic regression analysis (F-LRA) techniques assess the adolescent's mental health conditions. Finally, our proposed work's performance is examined and compared with classical techniques to gain our work with the greatest effectiveness. Findings are depicted in chart formation by employing the MATLAB tool.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyt.2022.902456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199859PMC
May 2022

Electroacupuncture at Zusanli Alleviates Sepsis by Regulating the TLR4-MyD88-NF-Kappa B Pathway and Diversity of Intestinal Flora.

Evid Based Complement Alternat Med 2022 10;2022:6706622. Epub 2022 Jun 10.

Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, China.

Background: Electroacupuncture (EA) at the Zusanli acupoint (ST36) has shown therapeutic potential for sepsis due to its ability to limit inflammation and to regulate gastrointestinal tract symptoms. However, the mechanisms contributing to the effects of EA at ST36 on sepsis and connections with the intestinal flora remain unclear. This study was designed to explore the effects of EA at ST36 on Toll-like receptor 4 signaling and the intestinal flora.

Methods: ICR mice were randomly divided into 4 groups: control group, model group, EA group, and sham EA group. EA at ST36 was performed at 2.5 mA and 2 to 100 Hz, and the 30 min of dense wave was achieved over 5 days. A sepsis model was built by intraperitoneal injection of lipopolysaccharide (LPS, 10 mg/mL). The levels of expression of interleukin-1 (IL-1), IL-6, tumor necrosis factor- (TNF-), and IL-10 were detected by enzyme-linked immunosorbent assays, and lactate dehydrogenase (LDH) levels in serum were measured by biochemical tests. Expression levels of Bax, Bcl2, cleaved caspase-3, Toll-like receptor (TLR4), nuclear factor-kappa B (NF-B), and myeloid differentiation factor 88 (MyD88) were assessed by the Western blotting. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining was used to evaluate apoptosis. The intestinal microecology was assessed via 16S rRNA gene sequencing.

Results: EA at ST36 reduced the expression of IL-1, IL-6, and TNF- and increased the expression of IL-10 to inhibit the inflammatory response. EA at ST36 also inhibited apoptosis, as measured by TUNEL staining, and decreased the Bax/Bcl2 ratio and levels of caspase-3 and cleaved caspase-3, as well as LDH release. Our results suggest that alleviation of sepsis may correlate with the downregulation of levels of TLR4, NF-B, and MyD88. Importantly, EA at ST36 improved the diversity of the intestinal flora and increased the abundance of Firmicutes and Actinobacteria. Conclusion. EA at ST36 prevented sepsis from worsening by inhibiting inflammation and apoptosis, which correlated with the regulation of the TLR4/NF-B/MyD88 signaling axis and modulation of the intestinal flora.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/6706622DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205730PMC
June 2022

Phillygenin ameliorates nonalcoholic fatty liver disease via TFEB-mediated lysosome biogenesis and lipophagy.

Phytomedicine 2022 Aug 3;103:154235. Epub 2022 Jun 3.

Beijing Key Laboratory for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China. Electronic address:

Background: Lipophagy is an autophagic process, which delivers the intracellular lipid droplets to the lysosomes for degradation. Recent studies revealed that the impairment of lysosomal biogenesis and autophagic flux led to dysregulation of lipophagy in hepatocytes, which exacerbated the development of nonalcoholic fatty liver disease (NAFLD). Therefore, agents restoring autophagic flux and lipophagy in hepatocytes may have therapeutic potential against this increasingly prevalent disease. Phillygenin (PHI), a lignin extracted from Forsythia suspense, exerts hepatoprotective and anti-inflammatory effects. However, the effect of PHI on NAFLD remains unknown.

Purpose: This study aimed to investigate the protective effect of PHI on NAFLD and elucidate the underlying mechanism.

Methods: The effects of PHI were examined in palmitate (PA)-stimulated AML12 cells and primary hepatocytes, as well as in NAFLD mice induced by a high-fat diet (HFD). We also used transcription factor EB (TFEB) knockdown hepatocytes and hepatocyte-specific TFEB knockout (TFEB) mice for mechanistic studies. In vivo and in vitro studies were performed using western blots, immunofluorescence techniques, and transmission electron microscopy.

Results: Our results indicated that autophagic flux and lysosome biogenesis in PA-stimulated hepatocytes were impaired. PHI alleviated lipid deposition by increasing lysosomal biogenesis and autophagic flux. It also stimulated the release of endoplasmic reticulum Ca to activate calcineurin, which regulated TFEB dephosphorylation and nuclear translocation, and promoted lysosomal biogenesis. In addition, PHI blocked the NLRP3 inflammasome pathway and improved hepatocyte inflammation in an autophagy-dependent manner. Consistent with the in vitro results, PHI improved hepatic steatosis and inflammation in HFD mice, but these beneficial effects were eliminated in hepatocyte-specific TFEB knockout mice.

Conclusion: Despite PHI has been reported to have anti-hepatic fibrosis effects, whether it has a hepatoprotective effects against NAFLD and the underlying molecular mechanism remain unclear. Herein, we found that PHI restored lipophagy and suppressed lipid accumulation and inflammation by regulating the Ca-calcineurin-TFEB axis in hepatocytes. Thus, PHI represents a therapeutic candidate for the treatment of NAFLD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2022.154235DOI Listing
August 2022

Tumor-associated macrophages promote epithelial-mesenchymal transition and the cancer stem cell properties in triple-negative breast cancer through CCL2/AKT/β-catenin signaling.

Cell Commun Signal 2022 Jun 17;20(1):92. Epub 2022 Jun 17.

Guangzhou Key Laboratory of "Translational Medicine on Malignant Tumor Treatment", Affiliated Cancer Hospital & Institute of Guangzhou Medical University, No.78 Hengzhigang Road, Guangzhou, 510095, Guangdong, China.

Background: Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer with poor prognosis and limited treatment. As a major component of the tumor microenvironment, tumor-associated macrophages (TAMs) play an important role in facilitating the aggressive behavior of TNBC. This study aimed to explore the novel mechanism of TAMs in the regulation of epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) properties in TNBC.

Methods: Expression of the M2-like macrophage marker CD163 was evaluated by immunohistochemistry in human breast cancer tissues. The phenotype of M2 macrophages polarized from Tohoku-Hospital-Pediatrics-1 (THP1) cells was verified by flow cytometry. Transwell assays, wound healing assays, western blotting, flow cytometry, ELISA, quantitative polymerase chain reaction (qPCR), luciferase reporter gene assays, and immunofluorescence assays were conducted to investigate the mechanism by which TAMs regulate EMT and CSC properties in BT549 and HCC1937 cells.

Results: Clinically, we observed a high infiltration of M2-like tumor-associated macrophages in TNBC tissues and confirmed that TAMs were associated with unfavorable prognosis in TNBC patients. Moreover, we found that conditioned medium from M2 macrophages (M2-CM) markedly promoted EMT and CSC properties in BT549 and HCC1937 cells. Mechanistically, we demonstrated that chemokine (C-C motif) ligand 2 (CCL2) secretion by TAMs activated Akt signaling, which in turn increased the expression and nuclear localization of β-catenin. Furthermore, β-catenin knockdown reversed TAM-induced EMT and CSC properties.

Conclusions: This study provides a novel mechanism by which TAMs promote EMT and enhance CSC properties in TNBC via activation of CCL2/AKT/β-catenin signaling, which may offer new strategies for the diagnosis and treatment of TNBC. Video Abstract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12964-022-00888-2DOI Listing
June 2022

Sex differences in arterial identity correlate with neointimal hyperplasia after balloon injury.

Mol Biol Rep 2022 Jun 17. Epub 2022 Jun 17.

Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT, USA.

Background: Endovascular treatment of atherosclerotic arterial disease exhibits sex differences in clinical outcomes including restenosis. However, sex-specific differences in arterial identity during arterial remodeling have not been described. We hypothesized that sex differences in expression of the arterial determinant erythropoietin-producing hepatocellular receptor interacting protein (Ephrin)-B2 occur during neointimal proliferation and arterial remodeling.

Methods And Results: Carotid balloon injury was performed in female and male Sprague-Dawley rats without or 14 days after gonadectomy; the left common carotid artery was injured and the right carotid artery in the same animal was used as an uninjured control. Arterial hemodynamics were evaluated in vivo using ultrasonography pre-procedure and post-procedure at 7 and 14 days and wall composition examined using histology, immunofluorescence and Western blot at 14 days after balloon injury. There were no significant baseline sex differences. 14 days after balloon injury, there was decreased neointimal thickness in female rats with decreased smooth muscle cell proliferation and decreased type I and III collagen deposition, as well as decreased TNFα- or iNOS-positive CD68+ cells and increased CD206- or TGM2-positive CD68+ cells. Female rats also showed less immunoreactivity of VEGF-A, NRP1, phosphorylated EphrinB2, and increased Notch1, as well as decreased phosphorylated Akt1, p38 and ERK1/2. These differences were not present in rats pretreated with gonadectomy.

Conclusions: Decreased neointimal thickness in female rats after carotid balloon injury is associated with altered arterial identity that is dependent on intact sex hormones. Alteration of arterial identity may be a mechanism of sex differences in neointimal proliferation after arterial injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-022-07644-2DOI Listing
June 2022

Effect of Delphinidin on Metabolomic Profile in Breast Carcinogenesis.

Anticancer Agents Med Chem 2022 Jun 16. Epub 2022 Jun 16.

School of Public Health, Chengdu Medical College, Chengdu, China.

Background: Breast cancer is a malignant tumor which threat to women's physical and mental health. Delphinidin, one of the main anthocyanidins, has potent anti-cancer properties. In previous study, we found that delphinidin has the preventive role in MNU-induced breast carcinogenesis of rats, but the molecular mechanism by which delphinidin combats breast cancer has not been completely elucidated.The aim of the present study was to identify metabolic profile that account for delphinidin on the preventive effect on 1-methyl-1-nitrosourea (MNU)-induced breast carcinogenesis of rats.

Methods: In the present study, liquid chromatography-mass spectrometry (LC-MS) was conducted to identify metabolic profiles of rat tissues collected from normal mammary glands (normal group), breast tumors derived from MNU-induced breast carcinogenesis models (control group) and delphinidin administration models (delphinidin group). Principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA) were employed to identify biochemical patterns. The values of variable importance in the projection (VIP) in PLS-DA model combined with the P value of Student's t-test were used to determine important metabolites. An orthogonal partial least square discriminant analysis (OPLS-DA) was used to conduct the supervised analysis. The fitness and prediction capabilities of PCA modes were measured by R 2 and Q 2 value respectively. Potential biomarkers were subjected to pathway analysis with Metaboanalyst 3.0 based on the KEGG Pathway Database to identify related metabolic pathways.

Results: The PCA and PLS-DA analysis indicated that the proposed method were satisfactory for metabolomic analysis. Metabolites from the obtained features were further filtered by PLS-DA analysis with VIP>1.0 and P<0.05. The significant difference was appeared in 190 metabolites between normal group and control group (P<0.05). Eight most significant metabolic pathways were obtained on the basis of the results of P<0.05 data analysis between control and normal group, embodying in aminoacyl-tRNA biosynthesis, arginine biosynthesis, biosynthesis of unsaturated fatty acids, valine, leucine and isoleucine biosynthesis, purine metabolism, alanine, aspartate and glutamate metabolism, glycerophospholipid metabolism, histidine metabolism. A total of 48 metabolites were identified to be associated with protective effects of delphinidin on MNU-induced rats significantly(P<0.05). Compared with control group, a total of 5 metabolic pathways were significantly perturbed in response to delphinidin administration (p<0.05), including in taurine and hypotaurine metabolism, Glycerophospholipid metabolism, arachidonic acid metabolism, aminoacyl-tRNA biosynthesis and primary bile acid biosynthesis.

Conclusion: Metabolites and metabolic pathways were identified to be associated with protective effects of delphinidin on MNU-induced rats. The findings provided new insights into the precise mechanism of delphinidin in preventing breast carcinogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2174/1871520622666220616101659DOI Listing
June 2022

Molecular and Supramolecular Approach to Highly Photocytotoxic Phthalocyanines with Dual Cell Uptake Pathways and Albumin-Enhanced Tumor Targeting.

ACS Appl Mater Interfaces 2022 Jun 16. Epub 2022 Jun 16.

College of Chemistry, State Key Laboratory of Photocatalysis on Energy and Environment, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Fuzhou University, Fuzhou 350108, China.

Phototherapy for non-invasive cancer treatment has been extensively studied. An urgent challenge in phototherapy application is to fabricate appropriate targeted agents to achieve efficient therapeutic effect. Herein, a molecular and supramolecular approach for targeting phototherapy was reasonably designed and realized through the axial sulfonate modification of silicon(IV) phthalocyanines (Pcs), followed by supramolecular interaction with albumin. This approach can not only improve the photoactivities (, fluorescence emission and reactive oxygen species production) of the Pcs but also enhance their tumor targeting. Most importantly, one of the deigned Pcs () can target HepG2 cells through dual cell pathways, leading to an extremely high phototoxicity with an EC (, concentration of Pcs to kill 50% of cells under light irradiation) value of 2.0 nM. This finding presents a feasible strategy to realize efficient targeting phototherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.2c05814DOI Listing
June 2022

Structure-Directing Role of Support on Hg Oxidation over VO/TiO Catalyst Revealed for NO and Hg Simultaneous Control in an SCR Reactor.

Environ Sci Technol 2022 Jun 16. Epub 2022 Jun 16.

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, P. R. China.

The crystal structure of TiO strongly influences the physiochemical properties of supported active sites and thus the catalytic performance of the as-synthesized catalyst. Herein, we synthesized TiO with different crystal forms (R = rutile, A = anatase, and B = brookite), which were used as supports to prepare vanadium-based catalysts for Hg oxidation. The Hg oxidation efficiency over VO/TiO-B was the best, followed by VO/TiO-A and VO/TiO-R. Further experimental and theoretical results indicate that gaseous Hg reacts with surface-active chlorine species produced by the adsorbed HCl and the reaction orders of Hg oxidation over VO/TiO catalyst with respect to HCl and Hg concentration were approximately 0 and 1, respectively. The excellent Hg oxidation efficiency over VO/TiO-B can be attributed to lower redox temperature, larger HCl adsorption capacity, and more oxygen vacancies. This work suggests that to achieve the best simultaneous removal of NO and Hg on state-of-the-art VO/TiO catalyst, a combination of anatase and brookite TiO-supported vanadyl tandem catalysts is supposed to be employed in the SCR reactor, and the brookite-type catalyst should be on the downstream of the anatase-based catalyst due to the inhibition of NH on Hg oxidation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.2c01480DOI Listing
June 2022

Insights into the Role of Macrophage Polarization in the Pathogenesis of Osteoporosis.

Oxid Med Cell Longev 2022 6;2022:2485959. Epub 2022 Jun 6.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.

Millions of people worldwide suffer from osteoporosis, which causes bone fragility and increases the risk of fractures. Osteoporosis is closely related to the inhibition of osteogenesis and the enhancement of osteoclastogenesis. In addition, chronic inflammation and macrophage polarization may contribute to osteoporosis as well. Macrophages, crucial to inflammatory responses, display different phenotypes under the control of microenvironment. There are two major phenotypes, classically activated macrophages (M1) and alternatively activated macrophages (M2). Generally, M1 macrophages mainly lead to bone resorption, while M2 macrophages result in osteogenesis. M1/M2 ratio reflects the "fluid" state of macrophage polarization, and the imbalance of M1/M2 ratio may cause disease such as osteoporosis. Additionally, antioxidant drugs, such as melatonin, are applied to change the state of macrophage polarization and to treat osteoporosis. In this review, we introduce the mechanisms of macrophage polarization-mediated bone resorption and bone formation and the contribution to the clinical strategies of osteoporosis treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/2485959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192196PMC
June 2022

Demonstration of enhanced direct-drive implosion efficiency using gradient pulses.

Phys Rev E 2022 May;105(5):L053203

Key Laboratory for Laser Plasmas (MoE), School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240, China.

Higher implosion efficiency is of great significance in direct-drive fusion research. We demonstrated the critical role played by the intensity gradient of the main drive laser pulse in improving efficiency of direct-drive implosions, using a double-gradient nanosecond pulse. Compared with a square pulse, the burn-through depth was increased by over 200%, and the shell velocity was increased by ∼2.1 times with an optimized double-gradient pulse. As the result, the implosion efficiency was enhanced by ∼ six times. It was found that by limiting the intensity gradient of the main drive pulse to no more than ∼2.5×10^{15}W/(cm^{2}ns), heat flux inhibition by nonlocal electron thermal transport effects could be eliminated, and ultimately an efficient mass ablation process was achieved. These results have relevance for pulse designs in ignition-scale direct-drive implosions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1103/PhysRevE.105.L053203DOI Listing
May 2022

Multiple instabilities of thermocapillary flow in a cylindrical pool with a rotating disk on the free surface.

Phys Rev E 2022 May;105(5-2):055101

Chongqing Urban Construction Investment (Group) Co., Ltd., Chongqing 400015, China.

The thermocapillary flow instabilities of silicon melt in a cylindrical pool with a rotating disk on the free surface (a simplified model of the Czochralski crystal growth) are numerically investigated by using the linear stability analysis. The complete neutral or critical stability curves are determined. Results show that the neutral stability curves form a closed region in the parameter plane, in which the steady axisymmetric flow is linearly stable. Two types of rotating wave (RW1 and RW2) instabilities and two types of hydrothermal wave (HTW1 and HTW2) instabilities are found. The energy analysis shows that all the instabilities are hydrodynamic (inertial) in nature. Specifically, RW1 and RW2 are caused by the azimuthal shear induced by the crystal rotation, while HTW1 and HTW2 are caused by the radial shear induced by the thermocapillary force.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1103/PhysRevE.105.055101DOI Listing
May 2022

IL-4 and CCR7 play an important role in the development of keloids in patients with a family history.

Am J Transl Res 2022 15;14(5):3381-3394. Epub 2022 May 15.

Department of Plastic Surgery, Peking Union Medical College Hospital Beijing, China.

Background: The occurrence of keloids tends to show family clusters and more severe symptoms in patients with a family history of the condition, but their pathological mechanisms remain unclear. In this study, we aimed to investigate the differences in genetic susceptibility between keloid patients with a family history of keloids and sporadic patients with sporadic keloids and explore potential therapeutic targets of keloids at the molecular level.

Methods: High-throughput sequencing data were obtained from normal skin tissue of patients with a family history of keloids (FN group) and normal skin tissue from sporadic patients (N group). Bioinformatics analysis was employed to identify hub genes. Promising hub genes were identified using RT-qPCR, immunohistochemistry and immunofluorescence assays, and Western blotting. GO and KEGG pathway enrichment analysis was performed to determine the main functions between the two groups.

Results: Patients with a family history of keloids had more severe clinical symptoms (Ρ = -0.749, P < 0.001). The expression of IL-4 and CCR7 was significantly different between patients with a family history of keloids and those with sporadic keloids. The high expression of IL-4 and the low expression of CCR7 in the FN group may be of key importance in explaining why keloids run in families (P < 0.05).

Conclusion: Having a family history of keloids is a risk factor for increased severity of keloids. IL-4 and CCR7 play an important role in the development of keloids in patients with a family history of the condition and may represent new targets for the treatment of keloids.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185030PMC
May 2022

SIRT1 ameliorates renal ischemia-reperfusion injury through suppressing endoplasmic reticulum stress-mediated autophagy.

Am J Transl Res 2022 15;14(5):3419-3429. Epub 2022 May 15.

Department of Organ Transplantation, Shanghai Changzheng Hospital, Navy Medical University Shanghai, China.

Background: Renal ischemia-reperfusion (IR) injury is a therapeutic challenge for surgeons. Sirtuin 1 (SIRT1) is an NAD-dependent deacetylase that plays a vital role in modulating cellular senescence and aging. In this study, we determined whether SIRT1 upregulation could alleviate renal IR injury and the underlying mechanism.

Methods: A renal IR model was induced in male C57BL/6 mice. Blood urea nitrogen and serum creatinine were evaluated as markers of kidney function, and renal injury was assessed by pathological examination. The inflammatory milieu was analyzed by real-time RT-PCR and myeloperoxidase immunofluorescence assays. Western blotting was used to quantify SIRT1 protein expression, endoplasmic reticulum stress, and autophagy.

Results: SIRT1 was upregulated in renal tissue after IR. Blood analysis and histopathologic examination demonstrated that SIRT1 preserved renal function and reduced renal damage. Further evaluation illustrated that IR induced autophagy and endoplasmic reticulum stress, while SIRT1 upregulation reduced endoplasmic reticulum stress-mediated autophagy levels.

Conclusions: SIRT1 upregulation protects the kidney against IR-induced injury by inhibiting endoplasmic reticulum stress-mediated autophagy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185025PMC
May 2022

Direct identification of A-to-I editing sites with nanopore native RNA sequencing.

Nat Methods 2022 Jun 13. Epub 2022 Jun 13.

School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.

Inosine is a prevalent RNA modification in animals and is formed when an adenosine is deaminated by the ADAR family of enzymes. Traditionally, inosines are identified indirectly as variants from Illumina RNA-sequencing data because they are interpreted as guanosines by cellular machineries. However, this indirect method performs poorly in protein-coding regions where exons are typically short, in non-model organisms with sparsely annotated single-nucleotide polymorphisms, or in disease contexts where unknown DNA mutations are pervasive. Here, we show that Oxford Nanopore direct RNA sequencing can be used to identify inosine-containing sites in native transcriptomes with high accuracy. We trained convolutional neural network models to distinguish inosine from adenosine and guanosine, and to estimate the modification rate at each editing site. Furthermore, we demonstrated their utility on the transcriptomes of human, mouse and Xenopus. Our approach expands the toolkit for studying adenosine-to-inosine editing and can be further extended to investigate other RNA modifications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41592-022-01513-3DOI Listing
June 2022

A biomarker signature to predict complete response to itacitinib and corticosteroids in acute graft-versus-host disease.

Br J Haematol 2022 Jun 11. Epub 2022 Jun 11.

Incyte Research Institute, Wilmington, Delaware, USA.

A broad proteomic analysis was conducted to identify and evaluate candidate biomarkers potentially predictive of response to treatment with an oral selective Janus kinase 1 (JAK1) inhibitor, itacitinib, in acute graft-versus-host disease (GVHD). Plasma samples from 25 participants (identification cohort; NCT02614612) were used to identify novel biomarkers that were tested in a validation cohort from a placebo-controlled, randomised trial (n = 210; NCT03139604). The identification cohort received corticosteroids plus 200 or 300 mg itacitinib once daily. The validation cohort received corticosteroids plus 200 mg itacitinib once daily or placebo. A broad proteomic analysis was conducted using a proximity extension assay. Baseline and longitudinal comparisons were performed with unpaired t-test and one-way analysis of variance used to evaluate biomarker level changes. Seven candidate biomarkers were identified. Monocyte-chemotactic protein (MCP)3, pro-calcitonin/calcitonin (ProCALCA/CALCA), together with a previously identified prognostic acute GVHD biomarker, regenerating islet-derived protein (REG)3A, stratified complete responders from non-responders (participants with progressive disease) to itacitinib, but not placebo, potentially representing predictive biomarkers of itacitinib in acute GVHD. ProCALCA/CALCA, suppressor of tumorigenicity (ST)2, and tumour necrosis factor receptor (TNFR)1 were significantly reduced over time by itacitinib in responders, potentially representing response-to-treatment biomarkers. Novel biomarkers have the potential to identify patients with acute GVHD that may respond to itacitinib plus corticosteroid treatment (NCT02614612; NCT03139604).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/bjh.18300DOI Listing
June 2022

Value of Magnetic Resonance Images and Magnetic Resonance Spectroscopy in Diagnosis of Brain Tumors under Fuzzy C-Means Algorithm.

Contrast Media Mol Imaging 2022 30;2022:3315121. Epub 2022 May 30.

Department of Radiology, Zibo Central Hospital, Zibo 255000, Shandong, China.

This study was aimed to explore the diagnostic value of magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) in brain tumors under the fuzzy C-means (FCM) algorithm. The two-dimensional FCM hybrid algorithm was improved to be three-dimensional. The MRI images and MRS spectra of 127 patients with brain tumors (low-grade glioma group) and 54 healthy people (healthy group) were analyzed. The results suggested that the membership matrix of the improved algorithm had lower ambiguity, higher segmentation accuracy, closer relationship of intrapixels, and stronger irrelevance of interclass pixels. Through the analysis of gray matter volume, it was found that, compared with the healthy group, the gray matter and white matter volumes in the brain of high-grade glioma were higher, and those of low-grade glioma group were lower. The improved FCM algorithm could obtain a higher accuracy of 88.64% in segmenting images. It had a higher sensitivity to gray matter changes in brain tumors, reaching 92.72%; its specificity was not much different from that of traditional FCM, which were 83.61% and 88.06%, respectively. In the diagnostic value, the area under the curve of mean kurtosis was the largest, which was 0.962 ( < 0.001). The best critical value was 0.4096, which had a greater reference significance for clinical treatment and prognosis. The ratio of choline/N-acetyl-aspartate and the ratio of choline/creatine also showed significant differences in high- and low-grade gliomas ( < 0.05), but the specificity and sensitivity were slightly lower. It also had guiding significance for the grading of gliomas. Overall, the improved FCM algorithm had obvious advantages in the segmentation process of MRI images, which provided help for the clinical diagnosis of brain tumors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/3315121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170444PMC
June 2022

Comparison of Anterior and Posterior Approaches for Acute Traumatic Central Spinal Cord Syndrome with Multilevel Cervical Canal Stenosis without Cervical Fracture or Dislocation.

Int J Clin Pract 2022 16;2022:5132134. Epub 2022 Feb 16.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.

Introduction: This is a retrospective comparative study that aims to compare the benefits of different surgical approaches for patients with multilevel cervical canal stenosis (CCS) without cervical fracture or dislocation of acute traumatic central cord syndrome (ATCCS).

Methods: From January 2015 to December 2018, 59 patients were included in the study. Among them, 35 patients (Group A) received anterior surgery and 24 patients (Group B) received posterior surgery. Primary outcome measures were American Spinal Cord Injury Association (Asia) grade, Japanese Orthopaedic Association (JOA) score, and recovery rate (RR). Secondary outcome measures included operation time, intraoperative blood loss, visual analogue scale (VAS) score, cervical sagittal parameters, and complications. Multivariate linear regression was used to analyze prognostic determinants.

Results: Compared with Group B, Group A had longer operation time and more intraoperative blood loss ( < 0.05). However, the VAS score of Group B was higher than that of Group A at discharge ( < 0.05). There was no significant difference in cervical sagittal plane parameters between the two groups ( > 0.05). Postoperative complications were different in the two groups. During follow-up, the Asia grade, the JOA score, and RR of both groups improved ( < 0.05), but there were no significant differences between the two groups ( > 0.05). Younger age, earlier surgery, and better preoperative Asia grade were correlated with better prognosis.

Conclusions: For patients with multilevel CCS without cervical fracture or dislocation of ATCCS, both surgical approaches had good outcomes. Although no significant differences were found in the primary outcome measures between the two groups, there were different recommendations for the secondary outcome measures. Younger age, earlier surgery, and better preoperative Asia grade were protective factors for better prognosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/5132134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159116PMC
June 2022

Effect of Different Anesthesia and Puncture Methods of Percutaneous Kyphoplasty on More Than 90-Year-Old Osteoporotic Vertebral Fracture: Advantages of the ERAS Concept.

Int J Clin Pract 2022 9;2022:7770214. Epub 2022 May 9.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.

Background: Percutaneous kyphoplasty (PKP) is an effective minimally invasive technique for the treatment of osteoporotic vertebral fracture (OVF) in recent years. This study focuses on the analysis of PKP surgery and anesthesia in osteoporotic vertebral facture patients over 90 years old with the concept of "enhanced recovery after surgery."

Methods: This study reviewed 239 patients who were diagnosed with OVF retrospectively between October 2015 and June 2019. According to the method of anesthesia, these patients were divided into Group A ( = 125) and Group B ( = 114). According to the pedicle puncture approach, these patients were divided into Group C ( = 102) and Group D ( = 137). The anterior vertebral height (AVH) and local kyphosis angle (LKA) were used to evaluate the degree of vertebral damage and restoration. The visual analogue scale (VAS) and the Oswestry Disability Index (ODI) scores were used for assessing functional outcomes. Some parameters were used to assess the perioperative conditions such as operation time, amount of bone cement perfusion, intraoperative fluoroscopy times, anesthesia recovery time, time out of the bed, hospital stay, hospitalization cost, and complications.

Results: The visual analogue scale (VAS), Oswestry Disability Index (ODI), anterior vertebral height (AVH), and local kyphosis angle (LKA) 1 day, 1 year after surgery, and at the last follow-up all showed significant improvement ( < 0.05) in comparison with those before surgery both in Groups A and B and Groups C and D. The ODI 1 day after surgery was significantly better in Group B than Group A ( < 0.05). Compared with Group B, Group A required longer time of anesthesia, operation time, anesthesia recovery time, time to get out of bed, and length of hospital stay and more hospitalization costs ( < 0.05). Group D required longer operation time, longer time to get out of bed, more bone cement volume, fluoroscopy time, and more operation hospitalization costs compared with Group C ( < 0.05).

Conclusion: We recommend unilateral puncture under local anesthesia for OVF in the patients aged over 90 from the perspective of rapid recovery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/7770214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159234PMC
June 2022

Fiber Bragg Grating-Based Smart Garment for Monitoring Human Body Temperature.

Sensors (Basel) 2022 Jun 2;22(11). Epub 2022 Jun 2.

School of Textile Science and Engineering, Tiangong University, Tianjin 300387, China.

Body temperature provides an insight into the physiological state of a person, and body temperature changes reflect much information about human health. In this study, a garment for monitoring human body temperature based on fiber Bragg grating (FBG) sensors is reported. The FBG sensor was encapsulated with a PMMA tube and calibrated in the thermostatic water bath. The results showed that FBG sensors had good vibration resistance, and the wavelength changed about 0-1 pm at a 0.5-80 Hz vibration frequency. The bending path of the optical fiber after integration with clothing is discussed. When the bending radius is equal to or greater than 20 mm, a lower bending loss can be achieved even under the bending and stretching of the human body. The FBG sensor, the optical fiber, and the garment were integrated together using hot melt glue by the electric iron and the hot press machine. Through experiments of monitoring human body temperature, the sensor can reach the human armpit temperature in about 10-15 min with the upper arm close to the torso. Because it is immune to electromagnetic interferences, the smart garment can be used in some special environments such as ultrasonography, magnetic resonance (MR), and aerospace.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s22114252DOI Listing
June 2022
-->