Publications by authors named "Hao Liang"

647 Publications

Highly Selective Entrapment of His-Tagged Enzymes on Superparamagnetic Zirconium-Based MOFs with Robust Renewability to Enhance pH and Thermal Stability.

ACS Biomater Sci Eng 2021 Jul 22. Epub 2021 Jul 22.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China.

Metal-organic frameworks (MOFs), as a kind of poriferous nanoparticle, are promising candidates for enzyme immobilization to enhance their stability and reusability. However, most MOFs could not specifically immobilize enzymes and regenerate easily, which inevitably leads to serious high consumption and environmental pollution. In this study, renewable and magnetic MOFs were first constructed to specially immobilize His-tagged enzymes from the cell lysates without purification. The immobilized β-glucuronidase exhibited wider pH adaptability and temperature stability. The relative activity of immobilized β-glucuronidase was still maintained at ∼80% after eight cycles. Importantly, after simple treatment, the immobilization capacity of regenerated MOFs after simple treatment was restored to more than 90% in the first three times. The specific magnetic MOFs were proven to be an efficient and renewable platform for one-step immobilization and purification of His-tagged enzymes, showing great potential in industrial applications of nanotechnology and biocatalysis.
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http://dx.doi.org/10.1021/acsbiomaterials.1c00780DOI Listing
July 2021

Single cell RNA-seq reveals genes vital to in vitro fertilized embryos and parthenotes in pigs.

Sci Rep 2021 Jul 13;11(1):14393. Epub 2021 Jul 13.

College of Animal Science, Yangtze University, Jingzhou, 434025, Hubei, China.

Successful early embryo development requires the correct reprogramming and configuration of gene networks by the timely and faithful execution of zygotic genome activation (ZGA). However, the regulatory principle of molecular elements and circuits fundamental to embryo development remains largely obscure. Here, we profiled the transcriptomes of single zygotes and blastomeres, obtained from in vitro fertilized (IVF) or parthenogenetically activated (PA) porcine early embryos (1- to 8-cell), focusing on the gene expression dynamics and regulatory networks associated with maternal-to-zygote transition (MZT) (mainly maternal RNA clearance and ZGA). We found that minor and major ZGAs occur at 1-cell and 4-cell stages for both IVF and PA embryos, respectively. Maternal RNAs gradually decay from 1- to 8-cell embryos. Top abundantly expressed genes (CDV3, PCNA, CDR1, YWHAE, DNMT1, IGF2BP3, ARMC1, BTG4, UHRF2 and gametocyte-specific factor 1-like) in both IVF and PA early embryos identified are of vital roles for embryo development. Differentially expressed genes within IVF groups are different from that within PA groups, indicating bi-parental and maternal-only embryos have specific sets of mRNAs distinctly decayed and activated. Pathways enriched from DEGs showed that RNA associated pathways (RNA binding, processing, transport and degradation) could be important. Moreover, mitochondrial RNAs are found to be actively transcribed, showing dynamic expression patterns, and for DNA/H3K4 methylation and transcription factors as well. Taken together, our findings provide an important resource to investigate further the epigenetic and genome regulation of MZT events in early embryos of pigs.
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http://dx.doi.org/10.1038/s41598-021-93904-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277874PMC
July 2021

Clinical significance of FBXW7 tumor suppressor gene mutations and expression in human colorectal cancer: a systemic review and meta-analysis.

BMC Cancer 2021 Jul 3;21(1):770. Epub 2021 Jul 3.

Department of General Surgery, Key Laboratory of Metabolism and Gastrointestinal Tumor, Key Laboratory of Laparoscopic Technology, Shandong Medicine and Health Key Laboratory of General Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, the First Affiliated Hospital of Shandong First Medical University, Jinan, 250000, Shandong, China.

Background: Various studies investigating the clinical significance of FBXW7 mutation and/or expression have yielded inconclusive results in colorectal cancer (CRC) patients. Therefore, the present meta-analysis summarizes previous evidence and evaluates the clinical significance, including the prognostic role, of FBXW7 status in CRCs.

Methods: The meta-analysis was conducted by searching the databases of PubMed, China National Knowledge Infrastructure (CNKI), WANFANG data, Web of Science, Embase, and Web of Science. Pooled odds ratios (ORs) and hazard ratios (HRs) and corresponding 95% confidence intervals (CIs) were calculated to assess the relationships between FBXW7 status and clinicopathological features and survival in CRC, respectively.

Results: Ten studies involving 4199 patients met the inclusion criteria and included in our meta-analysis. FBXW7 mutation/low expression was obviously correlated with advanced T stage (OR = 0.44, 95% CI: 0.27-0.74, P <  0.01) and lymph node metastasis (OR = 1.88, 95% CI: 1.40-2.53, P <  0.01), but was not associated with other parameters. Further investigation found that FBXW7 mutation/low expression predicted poor OS (HR = 1.25, 95% CI: 1.06-1.47, P <  0.01), but not DFS in CRC (HR = 1.04, 95% CI: 0.60-1.82, P = 0.88). Subgroup analysis found that FBXW7 status was obviously correlated with OS in cohorts recruited after 2009 (HR = 1.32, 95% CI: 1.17-1.50, P <  0.01), from eastern Asia (HR = 1.27, 95% CI: 1.04-1.55, P = 0.02), detected by immunohistochemistry/qRT-PCR (HR = 1.39, 95% CI: 1.22-1.59, P <  0.01), and analysed with multivariate method (HR = 1.47, 95% CI: 1.25-1.74, P <  0.01).

Conclusions: This study indicates that FBXW7 status, expression level especially, is associated with OS but not DFS in CRC. FBXW7 expression level may function as a prognostic biomarker in CRC.
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http://dx.doi.org/10.1186/s12885-021-08535-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254329PMC
July 2021

Immune marker signature helps to predict survival in uveal melanoma.

Math Biosci Eng 2021 May;18(4):4055-4070

Department of Ophthalmology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.

The detailed molecular function of tumor microenvironment (TEM) in uveal melanoma (UVM) remains unclear. This study generated the immune index and the stromal index scores by ESTIMATE algorithm based on RNA-sequencing data with 80 UVM patients. There was no correlation between the immune stromal index and clinical parameters. The differentially expressed genes related to the immune stromal index were calculated and were described by functional annotations and protein-protein interaction network diagrams. After univariate and multivariate Cox regression analyses, there were four genes (HLA-J, MMP12, HES6, and ADAMDEC1) with significant prognostic significance. The prognostic model was constructed using these four characteristic genes, and the KM curve and tROC curve were described to show that the model had a better ability to predict survival outcomes and prognosis. The verification results in GSE62075 showed that HLA-J and HES6 were expressed differently in the cancer group than in the non-cancer group. This study indicates that the risk signature based on the immune index can be used as an indicator to evaluate the prognosis of patients with UVM.
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http://dx.doi.org/10.3934/mbe.2021203DOI Listing
May 2021

Inhibitory mechanism of low-oxygen-storage treatment in postharvest internal bluing of radish (Raphanus sativus) roots.

Food Chem 2021 Jun 19;364:130423. Epub 2021 Jun 19.

Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing 100097, China. Electronic address:

Oxidative stress in radish roots causes internal blue discoloration and decreases vegetable quality. Accordingly, the effects of different oxygen concentration treatment on this coloration during storage was investigated; 4-hydroxyglucobrassicin content (a precursor of the blue component); the reactive oxygen species (ROS) superoxide (O) and hydrogen peroxide (HO); the antioxidants ascorbic acid (AsA) and glutathione (GSH); and the activities and gene expression levels of the enzymes catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), glutathione peroxidase (GPX), were monitored under normal and low-oxygen conditions. The results indicated that packaging radish roots under 10% O prevents blue discoloration by decreasing the activity and expression of the oxidant enzyme POD, increasing the levels of antioxidant and reducing substances, and upregulating antioxidant enzymes, all of which act to decrease the generation of ROS (O and HO).
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http://dx.doi.org/10.1016/j.foodchem.2021.130423DOI Listing
June 2021

Identification of Two Similar Novel HIV-1 Recombinant Forms (CRF01_AE/CRF07_BC) among Intravenous Drug Users in Guangxi, China.

Intervirology 2021 Jun 29:1-9. Epub 2021 Jun 29.

Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Universities Key Laboratory of Prevention and Control of Highly Prevalent Disease, School of Public Health, Guangxi Medical University, Nanning, China.

New kinds of HIV-1 circulating recombinant forms (CRFs) and unique recombinant forms (URFs) earn a great prevalence in China nowadays. In this study, we identified 2 similar URFs (2016GXNNIDU037 and 2019QZLSIDU253) both isolated from intravenous drug users (IDUs) in Guangxi, China. Phylogenetic analysis of the near full-length genome (NFLG) revealed 2 URFs both clustered with CRF01_AE but setting up a monophyletic branch, supporting a high bootstrap value. Bootscan analysis and subregional recombinant analysis found that the NFLG of 2016GXNNIDU037 and 2019QZLSIDU253 were both composed of CRF01_AE and CRF07_BC, with 3 CRF07_BC mosaic segments inserted into CRF01_AE backbones. The CRF01_AE segments of the 2 URFs clustered with a previously reported cluster 2 lineage of CRF01_AE. The 5 recombinant breakpoints of the 2 URFs were quite similar. Distinct from CRF01_AE/CRF07_BC URFs reported before, 2016GXNNIDU037 and 2019QZLSIDU253 are new evidence of a high genetic variety of HIV-1 in Guangxi, which may pose new challenges to HIV-1 prevention and molecular epidemiological surveillance in China.
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http://dx.doi.org/10.1159/000517052DOI Listing
June 2021

Elucidating the Phase Transformation and Metallization Behavior of Zinc Phosphide under High Pressure.

Inorg Chem 2021 Jul 27;60(14):10315-10322. Epub 2021 Jun 27.

Institute of Atomic and Molecular Physics, Sichuan University, Chengdu 610065, P. R. China.

Among the family of IIV-type compounds, zinc phosphide (ZnP) occupies a unique position. As one of the most promising semiconductors well-suited for photovoltaic applications, ZnP has attracted considerable attention. The stability of its structure and properties are of great interest and importance for science and technology. Here, we systematically investigate the pressurized behavior of ZnP using in situ synchrotron radiation angle-dispersive X-ray diffraction (ADXRD) and in situ electrical resistance measurement under high pressure. The ADXRD experiment shows that ZnP undergoes an irreversible structural phase transition under high pressure, beginning at 11.0 GPa and being completed at ∼17.7 GPa. Consistently, the high-pressure electrical resistance measurement reveals a pressure-induced semiconductor-metal transition for ZnP near 11.0 GPa. The kinetics of the phase transition is also studied using in situ electrical resistance measurement and can be well described by the classical Avrami model. What's more, the new high-pressure structure of ZnP is refined to be orthorhombic with space group ; the lattice parameters and bulk modulus of this high-pressure phase are determined as = 3.546 Å, = 5.004 Å, = 3.167 Å, and = 126.3 GPa. Interestingly, we also predict a possible structural phase transformation of orthorhombic phase () to cubic phase (432) during the decompression process; this cubic ZnP is metastable at ambient conditions. These experimental results reveal the unexpected high-pressure structural behaviors and electrical properties of ZnP, which could help to promote the further understanding and the future applications of ZnP as well as other IIV compounds.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00789DOI Listing
July 2021

Dysregulated Phenylalanine Catabolism Plays a Key Role in the Trajectory of Cardiac Aging.

Circulation 2021 Jun 24. Epub 2021 Jun 24.

INSERM U955, Université Paris-Est Créteil (UPEC), Créteil, France; AP-HP, Department of Physiology, Henri Mondor Hospital, FHU-SENEC, Créteil, France.

Aging myocardium undergoes progressive cardiac hypertrophy and interstitial fibrosis with diastolic and systolic dysfunction. Recent metabolomics studies shed light on amino acids in aging. The present study aimed to dissect how aging leads to elevated plasma levels of the essential amino acid phenylalanine (Phe) and how it may promote age-related cardiac dysfunction. We studied cardiac structure and function, together with Phe catabolism in wild-type (WT) and p21 mice (male; 2 to 24 months), the latter known to be protected from cellular senescence. To explore Phe's effects on cellular senescence and ectopic Phe catabolism we treated cardiomyocytes (primary adult rat or human AC-16) with Phe. To establish a role for Phe in driving cardiac aging, WT male mice were treated twice a day with Phe (200 mg/kg) for a month. We also treated aged WT mice with tetrahydrobiopterin (BH4; 10 mg/kg), the essential cofactor for the Phe-degrading enzyme phenylalanine hydroxylase (PAH), or restricted dietary Phe intake. The impact of senescence on hepatic Phe catabolism was explored in AML12 hepatocytes treated with Nutlin3a (a p53 activator), with or without p21-targeting siRNA or BH4, with quantification of PAH and tyrosine levels. Natural aging is associated with a progressive increase in plasma Phe levels concomitant with cardiac dysfunction, whilst p21 deletion delayed these changes. Phe treatment induced premature cardiac deterioration in young WT mice, strikingly akin to that occurring with aging, whilst triggering cellular senescence, redox and epigenetic changes. Pharmacological restoration of Phe catabolism with BH4 administration or dietary Phe restriction abrogated the rise in plasma Phe and reversed cardiac senescent alterations in aged WT mice. Observations from aged mice and human samples implicated age-related decline in hepatic Phe catabolism as a key driver of elevated plasma Phe levels and showed increased myocardial PAH-mediated Phe catabolism, a novel signature of cardiac aging. Our findings establish a pathogenic role for increased Phe levels in cardiac aging, linking plasma Phe levels to cardiac senescence via dysregulated Phe catabolism along a hepatic-cardiac axis. They highlight Phe/PAH modulation as a potential therapeutic strategy for age-associated cardiac impairment.
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http://dx.doi.org/10.1161/CIRCULATIONAHA.121.054204DOI Listing
June 2021

Implication of BAG5 downregulation in metabolic reprogramming of cisplatin-resistant ovarian cancer cells via mTORC2 signaling pathway.

Biochim Biophys Acta Mol Cell Res 2021 Aug 12;1868(9):119076. Epub 2021 Jun 12.

Department of Biochemistry & Molecular Biology, China Medical University, Shenyang 110026, China. Electronic address:

Ovarian cancer is the most frequent cause of gynecologic malignancies associated death. Primary or acquired cisplatin resistance is frequently occurred during ovarian cancer therapy. Cancer stem cells (CSC) tend to form minimal residual disease after chemotherapy and are implicated in relapse. The ability of cancer cells to reprogram their metabolism has recently been related with maintenance of CSC and resistance to chemotherapies. The current study found that BAG5 expression was decreased in cisplatin-resistant ovarian cancer cells and clinical tissues. Our data demonstrated that BAG5 knockdown was implicated in metabolic reprogramming and maintenance of cancer stem cell (CSC)-like features of ovarian cancer cells via regulation of Rictor and subsequent mTORC2 signaling pathway. In addition, the current study demonstrated that Bcl6 upregulation was responsible for repression of BAG5 transactivation via recruitment on the BAG5 promoter in cisplatin-resistant ovarian cancer. The current study also demonstrated reverse correlations between BAG5 and Bcl6, BAG5 and Rictor in ovarian serous adenocarcinoma tissues. Collectively, the current study identified the implication of Bcl6/BAG5/Rictor-mTORC2 signaling pathway in metabolic reprograming and maintenance of CSC-like features in cisplatin-resistant ovarian cancer cells. Therefore, further studies on the mechanism underlying regulation of metabolic reprogramming and CSC-like characteristics of cisplatin-resistant ovarian cancer cells may contribute to the establishment of novel therapeutic strategy for cisplatin-resistance.
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http://dx.doi.org/10.1016/j.bbamcr.2021.119076DOI Listing
August 2021

Transcriptome and metabolome profiling to elucidate mechanisms underlying the blue discoloration of radish roots during storage.

Food Chem 2021 Nov 11;362:130076. Epub 2021 May 11.

Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing 100097, China. Electronic address:

The internal blue discoloration of radish roots (Raphanus sativus) during storage affects their quality. We here performed transcriptome and metabolome profiling to investigate the mechanisms underlying the bluing of radish roots during storage. On comparing white radish (WR) and blue radish (BR), we identified 14,171 differentially expressed genes (upregulated: 7,383, downregulated: 6,788) and 145 differentially accumulated metabolites (upregulated: 117, downregulated: 28). Functional annotation analysis and metabolome profiling revealed that the blue discoloration of radish roots was promoted by high content of glucosinolates, oxidation system (ROS, CAT, POD) or low reduction system (GSH, GPX, APX, GST, ASA). Our results provide new insights into the underlying metabolic causes of the blue discoloration of radish roots and report candidate genes and metabolites involved in blue compound biosynthesis.
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http://dx.doi.org/10.1016/j.foodchem.2021.130076DOI Listing
November 2021

Monitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging.

Sci Rep 2021 Jun 1;11(1):11425. Epub 2021 Jun 1.

Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing, 100097, China.

Slicing may cause the risk of cross-contamination in cucumber. In this study, knife inoculated with Escherichia coli (E. coli) was used to cut cucumbers, bioluminescence imaging (BLI) was used to visualize the possible distribution and internalization of E. coli during cutting and storage. Results showed that the initial two slices resulted in greater bacterial transfer. The bacterial transfer exhibited a fluctuating decay trend, E. coli was most distributed at the initial cutting site. The contaminated area on the surface of cucumber slices decreased during the storage period, which can be attributed to the death and internalization of E. coli. The maximum internalization distance of E. coli was about 2-3 mm, and did not further spread after 30 min from inoculation. Hence, our results provide useful information for risk management in both home and industrial environment.
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http://dx.doi.org/10.1038/s41598-021-90584-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169731PMC
June 2021

Preparation, Characterization, and In Vivo Evaluation of Amorphous Icaritin Nanoparticles Prepared by a Reactive Precipitation Technique.

Molecules 2021 May 14;26(10). Epub 2021 May 14.

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

Icaritin is a promising anti-hepatoma drug that is currently being tested in a phase-III clinical trial. A novel combination of amorphization and nanonization was used to enhance the oral bioavailability of icaritin. Amorphous icaritin nanoparticles (AINs) were prepared by a reactive precipitation technique (RPT). Fourier transform infrared spectrometry was used to investigate the mechanism underlying the formation of amorphous nanoparticles. AINs were characterized via scanning electron microscopy, X-ray powder diffraction, and differential scanning calorimetry. Our prepared AINs were also evaluated for their dissolution rates in vitro and oral bioavailability. The resultant nanosized AINs (64 nm) were amorphous and exhibited a higher dissolution rate than that derived from a previous oil-suspension formulation. Fourier transform infrared spectroscopy (FTIR) revealed that the C=O groups from the hydrophilic chain of polymers and the OH groups from icaritin formed hydrogen bonds that inhibited AIN crystallization and aggregation. Furthermore, an oral administration assay in beagle dogs showed that C and AUC of the dried AINs formulation were 3.3-fold and 4.5-fold higher than those of the oil-suspension preparation ( < 0.01), respectively. Our results demonstrate that the preparation of amorphous drug nanoparticles via our RPT may be a promising technique for improving the oral bioavailability of poorly water-soluble drugs.
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http://dx.doi.org/10.3390/molecules26102913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155890PMC
May 2021

Fault Detection and Isolation of the Multi-Sensor Inertial System.

Micromachines (Basel) 2021 May 21;12(6). Epub 2021 May 21.

Beijing Aerospace Times Laser Inertial Technology Company, Ltd., Beijing 100094, China.

In order to solve the problem that the generalized likelihood test method cannot isolate the single fault of the four-gyro system and the double faults of the six-gyro system, a fault detection and isolation method combining the generalized likelihood test method with the residual error of the metabolism grey model is presented. The problem of isolating the single fault of the four-gyro system and the double faults of the six-gyro system using the generalized likelihood test method is analyzed. The method and process of fault detection and isolation are designed. The validity of the method presented in this paper is verified by simulation tests of the single fault of the four-gyro system and the double faults of the six-gyro system. By comparing the isolation performance with the generalized likelihood test method, it is proved that the isolation performance of the method proposed in this paper is better than that of the generalized likelihood test method. The method mentioned in this paper can effectively realize fault detection and isolation of the multi-gyro system and improve the inertial system's reliability.
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http://dx.doi.org/10.3390/mi12060593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224025PMC
May 2021

Combustion Performance of Spherical Propellants Deterred by Energetic Composite Deterring Agents.

ACS Omega 2021 May 11;6(20):13024-13032. Epub 2021 May 11.

School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

In order to achieve ideal burning progressivity and reduce harmful phenomena such as muzzle flame and smoke, energetic composite deterring agents (ECDAs) deterring spherical propellants were designed and prepared. The combustion performance of ECDA-deterred propellants was characterized by a closed vessel, and the interior ballistic performance was studied by a ballistic gun. High-speed photography and a smoke box were employed to capture muzzle flames and smoke. The results showed that triethylene glycol dinitrate (TEGDN) had a slight deterring effect weaker than that of poly(neopentyl glycol adipate) (PNA) on the propellants. The maximum pressure in the closed vessel bore of the ECDA-deterred propellants was 2.29 MPa higher than that of the dibutyl phthalate (DBP)-deterred propellants, though the - curve of the ECDA-deterred propellants was slightly lower and its combustion time was 0.44 ms longer. For ECDA containing 5 wt % PNA and 3.2 wt % TEGDN, 80 °C and 150 min are the best deterring conditions. The average velocity of the bullet propelled by ECDA-deterred propellants was increased by 93.4 m·s, while the average maximum pressure in the gun bore was decreased by 19 MPa, compared with the original propellants. The muzzle flame and smoke of the ECDA-deterred propellants were significantly reduced compared with the DBP-deterred propellants, where the smoke concentration was reduced by up to 44.5%.
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http://dx.doi.org/10.1021/acsomega.1c00637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158837PMC
May 2021

Integrated DNA Methylation and Gene Expression Analysis Identified S100A8 and S100A9 in the Pathogenesis of Obesity.

Front Cardiovasc Med 2021 13;8:631650. Epub 2021 May 13.

Department of Pathophysiology, School of Preclinical Medicine, Guangxi Medical University, Nanning, China.

To explore the association of DNA methylation and gene expression in the pathology of obesity. (1) Genomic DNA methylation and mRNA expression profile of visceral adipose tissue (VAT) were performed in a comprehensive database of gene expression in obese and normal subjects. (2) Functional enrichment analysis and construction of differential methylation gene regulatory networks were performed. (3) Validation of the two different methylation sites and corresponding gene expression was done in a separate microarray dataset. (4) Correlation analysis was performed on DNA methylation and mRNA expression data. A total of 77 differentially expressed mRNAs matched with differentially methylated genes. Analysis revealed two different methylation sites corresponding to two unique genes-s100a8-cg09174555 and s100a9-cg03165378. Through the verification test of two interesting different expression positions [differentially methylated positions (DMPs)] and their corresponding gene expression, we found that methylation in these genes was negatively correlated to gene expression in the obesity group. Higher S100A8 and S100A9 expressions in obese subjects were validated in a separate microarray dataset. This study confirmed the relationship between DNA methylation and gene expression and emphasized the important role of S100A8 and S100A9 in the pathogenesis of obesity.
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http://dx.doi.org/10.3389/fcvm.2021.631650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163519PMC
May 2021

Bardoxolone and bardoxolone methyl, two Nrf2 activators in clinical trials, inhibit SARS-CoV-2 replication and its 3C-like protease.

Signal Transduct Target Ther 2021 05 29;6(1):212. Epub 2021 May 29.

BNLMS, Peking-Tsinghua Center for Life Sciences at College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

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http://dx.doi.org/10.1038/s41392-021-00628-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164054PMC
May 2021

The feasibility of using Hcy, CRP, and Cys-C to analyze AMI patients' disease conditions and prognoses.

Am J Transl Res 2021 15;13(4):2724-2730. Epub 2021 Apr 15.

Department of Cardiology, Affiliated Hospital of Chengde Medical University Chengde City, Hebei Province, China.

Objective: This study was conducted to investigate the feasibility of using the serum homocysteine (Hcy), C-reactive protein (CRP), and cystatin C (Cys-C) levels to evaluate the conditions and prognoses of acute myocardial infarction (AMI) patients.

Methods: One hundred and twenty patients with AMI were enrolled as an observation group (the AMI group). Eighty patients with stable angina pectoris were included in a control group (the SA group). Eighty healthy volunteers were enrolled as a healthy control group (the NCHD group). The AMI patients were divided into groups of >20 (n = 32), 10-20 (n = 45) and <10 (n = 43) according to their APACHE-II scores. The groups were divided into a death group (n = 23) and a survival group (n = 97) according to the patients' 1-year follow-up outcomes. The differences in the serum Hcy, CRP, and Cys-C levels among the different groups, and the correlations between the serum levels were calculated.

Results: The serum Hcy, CRP, and Cys-C levels in the AMI group were significantly higher than they were in the SA and NCHD groups (<0.05), and the serum levels in the APACHE-II score >20 group were significantly higher than the serum levels in the 10-20 group. The dead group exhibited higher serum Hcy, CRP, and Cys-C levels than the survival group (<0.05), and our Spearman's correlation analysis showed that the serum Hcy, CRP and Cys-C levels were positively correlated with the APACHE II scores (r = 0.9157, r = 0.8519, r = 0.8598, <0.001). The area under curve of Hcy, CRP, and Cys-C for the AMI diagnoses were 0.9638 (95% CI: 0.9183-1.000), 0.8125 (95% CI: 0.6652-0.9598), and 0.7515 (95% CI: 0.5847-0.9184), respectively.

Conclusion: Serum Hcy, CRP, and Cys-C levels can reflect the severity of the patients' conditions.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129397PMC
April 2021

Predictive factors of viral load high-risk events for virological failure in HIV/AIDS patients receiving long-term antiviral therapy.

BMC Infect Dis 2021 May 18;21(1):448. Epub 2021 May 18.

Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning, 530021, Guangxi, China.

Background: In the era of anti-retroviral therapy (ART), the plasma HIV viral load (VL) is an important primary indicator for monitoring the HIV treatment response. To optimize the clinical management of HIV/AIDS patients, we investigated VL high-risk events related to virological failure (VF) and further explored the preventive factors of VL high-risk events.

Methods: The data were derived from China's HIV/AIDS Comprehensive Response Information Management System. HIV infected patients who initiated or received ART in Guangxi between 2003 and 2019 were included. The contributions of VL after 6 months of ART to VF and AIDS-related death were analysed by Kaplan-Meier curves, log-rank tests and Cox regression analyses. Both descriptive analyses and bivariate logistic regression were employed to further explore the preventive factors related to VL high-risk events of VF.

Results: The cumulative rates of VF in the high low-level viremia group (high LLV) (χ = 18.45; P <  0.001) and non-suppressed group (χ = 82.99; P <  0.001) were significantly higher than those in the viral suppression (VS) group. Therefore, the VL high-risk events of VF was defined as highest VL > 200 copies/ml after 6 months of ART. Compared with the VS group, the adjusted hazard risk was 7.221 (95% CI: 2.668; 19.547) in the high LLV group and 8.351 (95% CI: 4.253; 16.398) in the non-suppressed group. Compared with single patients, married or cohabiting (AOR = 0.591; 95% CI: 0.408, 0.856) and divorced or separated (AOR = 0.425, 95% CI: 0.207, 0.873) patients were negatively associated with VL high-risk events. So were patients acquired HIV homosexually (AOR = 0.572; 95% CI: 0.335, 0.978). However, patients who had ART modification were 1.728 times (95% CI: 1.093, 2.732) more likely to have VL high-risk events, and patients who used cotrimoxazole during ART were 1.843 times (95% CI: 1.271, 2.672) more likely to have VL high-risk events.

Conclusions: A VL greater than 200 copies/ml is a VL high-risk event for VF. Intervention measurements should be adopted to optimize the surveillance of ART in patients who are single or widowed, who have ART modification, and who use cotrimoxazole during ART.
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http://dx.doi.org/10.1186/s12879-021-06162-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130293PMC
May 2021

Transcriptome landscapes of multiple tissues highlight the genes involved in the flavor metabolic pathway in Chinese chive (Allium tuberosum).

Genomics 2021 Jul 13;113(4):2145-2157. Epub 2021 May 13.

Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs of China, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. Electronic address:

The unique flavor of Allium tuberosum is primarily associated with the hydrolysis of a series of organosulfur compounds, S-alk(en)yl cysteine sulphoxides (CSOs), upon tissue bruising or maceration. To obtain the tissue-specific transcriptomes, 18 RNA-Seq libraries representing leaf, root, stem, mature flower, inflorescence, and seed tissues of A. tuberosum were sequenced, finally yielding 133.7 Gb clean reads. The de novo assembled transcriptomes enabled the identification of 223,529 unigenes, which were functionally annotated and analyzed for the gene ontology and metabolic pathways. Furthermore, to reveal the flavor metabolic pathways, a total of 205 unigenes involved in the sulfur assimilation and CSO biosynthesis were identified, and their expression profiles were analyzed by RNA-Seq and qRT-PCR. Collectively, this study provides a valuable resource for in-depth molecular and functional researches especially on flavor formation, as well as for the development of molecular markers, and other genetic studies in A. tuberosum.
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http://dx.doi.org/10.1016/j.ygeno.2021.05.005DOI Listing
July 2021

Risk Management on Pre-Exposure Prophylaxis Adherence of Men Who Have Sex with Multiple Men: A Multicenter Prospective Cohort Study.

Risk Manag Healthc Policy 2021 28;14:1749-1761. Epub 2021 Apr 28.

School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, People's Republic of China.

Background: Men who have sex with multiple men (MSMM) belong to a high-risk group for HIV infection, and pre-exposure prophylaxis (PrEP) is an effective measure to prevent the infection. However, few studies on PrEP adherence by MSMM in China exist. We aimed to explore the protective motivation-related factors for PrEP adherence in an HIV-negative MSMM population in Western China and to provide a reference for future risk management and effective prevention strategies.

Methods: Data were collected from a 2-year follow-up cohort study of PrEP in MSM in China. Rogers' protective motivation theory (PMT) was used to study the PrEP adherence of MSMM, and logistic regression was performed to analyze the influencing factors of PrEP adherence.

Results: A total of 496 MSMM were included in the study: 299 (60.28%) of them in the good adherence group and 197 (39.72%) in the poor adherence group. The threat assessment scores of the good and poor adherence groups were 2.15 ± 0.59 and 2.06 ± 0.47, respectively, and the response assessment scores were 2.81 ± 0.62 and 2.74 ±0.62, respectively. Poor PrEP adherence was associated with on-demand PrEP medication (OR=0.670), students at school (OR=1.837), occasional condom use (OR=1.621), and good HIV knowledge (OR= 0.659). The higher the threat assessment score, the higher the susceptibility; and the lower the response cost, the stronger the protection motivation and the less likely MSMM were to have poor adherence.

Conclusion: On-demand PrEP medication is more conducive to adherence. Preventive management should focus on MSMMs who are students at school, those who occasionally use condoms, and those with poor HIV knowledge. Improving threat perception and susceptibility, and controlling and reducing the response cost can effectively improve PrEP adherence, and the subsequent application of PMT during intervention research can provide a reference for HIV prevention in MSMM.
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http://dx.doi.org/10.2147/RMHP.S295114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092636PMC
April 2021

Ectopic tooth in maxillary sinus compressing the nasolacrimal canal: A case report.

Medicine (Baltimore) 2021 May;100(18):e25514

Department of Stomatology.

Rationale: Ectopic tooth is tooth erupting out of normal anatomical position. Ectopic tooth can occur in different positions, such as maxillary sinus and nasal cavity. In this article, we present a rare case of an ectopic tooth with a dentigerous cyst in the maxillary sinus compressing the nasolacrimal canal.

Patient Concerns: An 8-year-old girl presented with a 2-month history of spontaneous lacrimation in her right eye. When she wept, more tear shed from her right eye than that from the left one. Computed tomographic (CT) imaging showed a huge low-density image containing a tooth in the maxillary sinus in her right maxilla; the right nasolacrimal canal vanished due to the compression of the ectopic tooth.

Diagnoses: Ectopic tooth with dentigerous cyst of right maxilla, and obstruction of nasolacrimal duct.

Interventions: The patient underwent nasal endoscopic maxillary sinus cystectomy.

Outcomes: The patient recovered well after cystectomy and has been symptom-free.

Lessons: The unique finding is that this is the first report about ectopic tooth compressing the nasolacrimal canal and inducing spontaneous lacrimation. Treatment: aspect: surgery under endoscope is a minimally invasive approach to ectopic tooth.
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http://dx.doi.org/10.1097/MD.0000000000025514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104135PMC
May 2021

Hepatitis C virus infection increases the risk of adverse outcomes following joint arthroplasty: A meta-analysis of observational studies.

Orthop Traumatol Surg Res 2021 Apr 27:102947. Epub 2021 Apr 27.

Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Shanghai Sixth People's Hospital, No.600 Yishan Road, Xuhui District, 200233 Shanghai, People's Republic of China.

Background: Whether hepatitis C virus (HCV)-positive patients are at risk for increased complications and long hospital stay following total joint arthroplasty (TJA) remains unclear. Therefore we performed a meta-analysis aiming to answer the following question: (1) are there differences in postoperative complications including joint infection and mortality between patients with or without hepatitis C following TJAs? (2) Are patients without HCV be associated with less blood loss, shorter hospital stay, lower readmission rate, higher function scores, lower revision and reoperation rates than patients with HCV?

Methods: A meta-analysis was conducted to pool data and quantitatively assessing the association between HCV infection and risks for adverse postoperative outcomes. A systematic search of all published studies concerning HCV and TJA was performed in five bibliographic databases, including PubMed, EMBASE, China National Knowledge Infrastructure, Web of Science, and the Cochrane Library databases. Random-effects meta-analysis of odds ratios (OR) was accomplished according to the classification of adverse events, subgroup analyses were performed based on surgery type.

Results: Fifteen eligible observational studies were included with a sample size of 9,788,166 patients. Overall pooled data revealed the increased risk of overall complications, including medical and surgical complications, in HCV-positive patients undergoing TJA compared with than in HCV-negative people (OR 1.57; 95%CI: 1.44-1.71 [p<0.00001]). Joint infections were highly common in HCV-positive patients undergoing lower-extremity TJA (OR 2.06; 95%CI: 1.73-2.47 [p<0.00001]). Furthermore, HCV infections were associated with high rates of reoperations and revisions (OR 1.47; 95%CI: 1.40-1.55 [p<0.00001]).

Conclusions: Patients with hepatitis C have an increased risk of adverse outcomes post-TJA and a high risk of reoperation and revision that is partially attributed to postoperative complications, particularly joint infections.

Level Of Evidence: III; systematic review and meta-analysis.
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http://dx.doi.org/10.1016/j.otsr.2021.102947DOI Listing
April 2021

TRIM29 alters bioenergetics of pancreatic cancer cells via cooperation of miR-2355-3p and DDX3X recruitment to AK4 transcript.

Mol Ther Nucleic Acids 2021 Jun 3;24:579-590. Epub 2021 Feb 3.

Department of Biochemistry and Molecular Biology, China Medical University, Shenyang 110026, China.

TRIM29 is dysregulated in pancreatic cancer and implicated in maintenance of stem-cell-like characters of pancreatic cancer cells. However, the exact mechanisms underlying oncogenic function of TRIM29 in pancreatic cancer cells remain largely unclarified. Using a global screening procedure, the current study found that adenylate kinase 4 (AK4) was profoundly reduced by TRIM29 knockdown. In addition, our data demonstrated that TRIM29 knockdown altered bioenergetics and suppressed proliferation and invasion of pancreatic cancer cells via downregulation of AK4 at the posttranscriptional level. The current study demonstrated that upregulation of microRNA-2355-3p (miR-2355-3p) upregulated AK4 expression via facilitating DDX3X recruitment to the AK4 transcript, and TRIM29 knockdown thereby destabilized the AK4 transcript via miR-2355-3p downregulation. Collectively, our study uncovers posttranscriptional stabilization of the AK4 transcript by miR-2355-3p interaction to facilitate DDX3X recruitment. Regulation of AK4 by TRIM29 via miR-2355-3p thereby provides additional information for further identification of attractive targets for therapy with pancreatic cancer.
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http://dx.doi.org/10.1016/j.omtn.2021.01.027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054099PMC
June 2021

Inhibition of receptor-interacting protein kinase-3 in the necroptosis pathway attenuates inflammatory bone loss in experimental apical periodontitis in Balb/c mice.

Int Endod J 2021 Apr 25. Epub 2021 Apr 25.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Sichuan, China.

Aim: To explore the role of necroptosis in apical periodontitis (AP), this study investigated necroptosis in a Fusobacterium nucleatum (Fn)-induced AP model of Balb/c mice and explored related intracellular signalling pathways in L929 cells affected by Fn.

Methodology: For the in vivo experiments, expression of receptor-interacting protein kinase-3 (RIP3) was inhibited using an adeno-associated virus and then the Balb/c mice model of AP was established by injecting Fn into the root canal of the first mandibular molars. Bone loss and number of osteoclasts were measured via micro-computed tomography and tartrate-resistant acid phosphatase staining, respectively; expression of RIP3 and phosphorylated mixed lineage kinase domain-like protein (pMLKL) was detected by immunohistochemistry and western blotting; expression of mRNA of inflammatory cytokines was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). For the in vitro experiments, L929 cells transfected with RIP3-Mus-siRNA or negative control siRNA were co-cultured with Fn; thereafter, western blotting, detection of cell death and viability and qRT-PCR analyses were performed to assess the activation of necroptosis pathway and expression of mRNA of inflammatory cytokines. Data were analysed with unpaired t-test and one-way analysis of variance with significance set at p < .05.

Results: The Fn-induced apical lesions were associated with apical bone loss, an increased number of osteoclasts, enhanced expression of pMLKL and increased mRNA levels of inflammatory cytokines(IL-1α and IL-1β); all these effects were alleviated by RIP3 inhibition (p < .05). L929 cells infected with Fn displayed increased expression of pMLKL and increased cell death (p < .05), together with decreased cell viability (p < .05), whilst transfection with RIP3-Mus-siRNA decreased the mRNA expression of inflammatory cytokines(TNF-α and IL-6, p < .05).

Conclusions: Necroptosis may be involved in AP progression. RIP3 inhibition ameliorated the expression of inflammatory cytokines and bone resorption in Fn-induced AP lesions in Balb/c mice.
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http://dx.doi.org/10.1111/iej.13534DOI Listing
April 2021

Quantitative tracking of endoplasmic reticulum viscosity during ferroptosis by an iridium complex TPPLM.

Chem Commun (Camb) 2021 May;57(41):5040-5042

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China.

Herein, we report a neutral iridium complex, [Ir(4-(2-pyridinyl)benzaldehyde)2(acetylacetone)] (Ir-ER), with viscosity-responsive phosphorescent emission intensity and lifetime. Quantitative measurement by two-photon phosphorescent lifetime imaging shows that the viscosity of ER increases significantly in the process of erastin-induced ferroptosis. Our work provides an effective strategy for quantitative measurement of the micro-environmental alternations of subcellular organelles during a specific cell death process.
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http://dx.doi.org/10.1039/d1cc01062jDOI Listing
May 2021

Is rice field a nitrogen source or sink for the environment?

Environ Pollut 2021 Aug 10;283:117122. Epub 2021 Apr 10.

Laboratory for Earth Surface Processes of the Ministry of Education, College of Urban and Environmental Sciences, Peking University, Beijing, 100871, PR China. Electronic address:

Rice field has been traditionally considered as a nonpoint source of reactive nitrogen (N) for the environment, while it, with surrounding ditches and ponds, also contributes to receiving N inputs from atmosphere and waterbodies and intercepting N outputs from rice field. However, a comprehensive assessment of the N source or sink of rice field for the environment is lacking. Here, we conducted the 3-year systematic observations and process-based simulations of N budget at the Jingzhou site in Central China. We identified the roles of rice field and evaluated the opportunities for shifting its role from N source (i.e., outputs > inputs) to sink (i.e., outputs ≤ inputs). Rice field was found to be a N source of 24.2-28.7 kg N ha for waterbodies (including surface and ground waters), but a N sink (2.2-8.8 kg N ha) for the atmosphere for the wet and normal year climatic scenarios. The "4R-nutrient stewardship" (i.e., using the right type of N fertilizers, at right rate, right time, and in right place) applied in rice field was sufficient for the source-to-sink shift for the atmosphere for dry year climatic scenario, but needed to implement together with improvements of irrigation and drainage for waterbodies. Furthermore, with the combination of these improved management technologies, rice field played a role as a N sink of up to 22.8 kg N ha for the atmosphere and up to 2.0 kg N ha for waterbodies, along with 24% decrease in irrigation water use and 21% decrease in N application rate without affecting rice yield and soil fertility. Together these findings highlight a possibility to achieve an environmental-friendly rice field by improving agricultural management technologies.
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http://dx.doi.org/10.1016/j.envpol.2021.117122DOI Listing
August 2021

Generation of VEGF knock-in Cashmere goat via the CRISPR/Cas9 system.

Int J Biol Sci 2021 2;17(4):1026-1040. Epub 2021 Mar 2.

State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010000, China.

Cashmere is a rare and specialised animal fibre, which grows on the outer skin of goats. Owing its low yield and soft, light, and warm properties, it has a high economic value. Here, we attempted to improve existing cashmere goat breeds by simultaneously increasing their fibre length and cashmere yield. We attempted this by knocking in the vascular endothelial growth factor () at the fibroblast growth factor 5() site using a gene editing technology and then studying its hair growth-promoting mechanisms. We show that a combination of RS-1 and NU7441 significantly improve the efficiency of CRISPR/Cas9-mediated, homologous-directed repair without affecting the embryo cleavage rate or the percentages of embryos at different stages. In addition, we obtained a cashmere goat, which integrated the gene at the site, and the cashmere yield and fibre length of this gene-edited goat were improved. Through next-generation sequencing, we found that the up-regulation of and the down-regulation of affected the cell cycle, proliferation, and vascular tone through the PI3K-AKT signalling pathway and at extracellular matrix-receptor interactions. Owing to this, the gene-edited cashmere goat showed impressive cashmere performance. Overall, in this study, we generated a gene-edited cashmere goat by integrating at the site and provided an animal model for follow-up research on hair growth mechanisms.
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http://dx.doi.org/10.7150/ijbs.55559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040296PMC
March 2021

Inhibition of regulator of G protein signaling 10, aggravates rheumatoid arthritis progression by promoting NF-κB signaling pathway.

Mol Immunol 2021 Jun 6;134:236-246. Epub 2021 Apr 6.

The State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Sichuan, People's Republic of China. Electronic address:

Rheumatoid arthritis (RA) is the most common inflammatory arthropathy, with evidence pointing to an immune-mediated etiology that propagates chronic inflammation. Although targeted immune therapeutics and aggressive treatment strategies have substantially improved, a complete understanding of the associated pathological mechanisms of the disease remains elusive. This study aimed at investigating whether regulator of G protein signaling 10 (RGS10) could affect rheumatoid arthritis (RA) pathology by regulating the immune response. A DBA/J1 mouse model of RA was established and evaluated for disease severity. RGS10 expression was inhibited by adeno-associated virus in vivo. Moreover, small interfering RNA was used to downregulate RGS10 expression in raw 264.7 cells in vitro. Results showed that RGS10 inhibition augmented RA severity, and attenuated the increase in expression of inflammatory factors. Furthermore, activated NF-κB signaling pathways were detected following RGS10 inhibition. These results revealed that RGS10 inhibition directly aggravated the RA pathological process by activating the NF-κB signaling pathway. Therefore, RGS10 is a promising novel therapeutic target for RA treatment with a potential clinical impact.
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http://dx.doi.org/10.1016/j.molimm.2021.03.024DOI Listing
June 2021

[Progress in Gene Therapy of Sickle Cell Disease Based on Hemoglobin F--Review].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Apr;29(2):643-647

Department of Biochemistry and Molecular Biology, Baotou Medical College, Baotou 014010, Inner Mongolia Autonomous Region, China,E-mail:

Sickle cell disease (SCD) is a single gene genetic disease, which seriously threatens the life span and quality of patients. On the basis of the pathogenesis of SCD and the alternative therapy based on fetal hemoglobin F (HbF), the research progress of transcription factors involved in the regulation of HbF gene expression, such as BCL11A, ZBTB7A, KLF-1, c-MYB and SOX6, as well as the application of CRISPR / Cas9, TALEN, zinc finger nuclease and other gene editing technologies in this field has been made, providing a solid theoretical basis for the exploration of new treatment schemes for β- like hemoglobin diseases, such as sickle cell disease and β- thalassemia.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.02.054DOI Listing
April 2021

Surface modification of the simvastatin factor-loaded silk fibroin promotes the healing of rotator cuff injury through β-catenin signaling.

J Biomater Appl 2021 Aug 28;36(2):210-218. Epub 2021 Mar 28.

159397Huashan Hospital Fudan University, Shanghai, China.

Rupture of the rotator cuff is a common injury of the shoulder joint in sports professionals. In addition, research on repair of the rotator cuff has gained popularity over the recent years. Given the high rate of re-tear after surgery, it is necessary to design and prepare biodegradable materials with good mechanical properties, for the management of the condition. Consequently, the present study conducted surface modification of the simvastatin factor-loaded silk fibroin for the repair of chronic rotator cuff injury in SD rats. The experiments were analyzed through scanning electron microscopy and the water contact angle. Additionally, the CCK-8 assay was used to observe the effect of the intervention on the proliferation of BMSCs. Moreover, the osteogenic differentiation of BMSCs was detected through the ALP and ARS assays while the expression of osteogenic genes was examined using qRT-PCR and Western blot analysis. Furthermore, a model for repairing chronic rotator cuff tears in SD rats was established . Thereafter, rotator cuff repair and healing were evaluated through HE staining while Masson and Sirius staining was used to detect the collagen formation ratio. Additionally, the study analyzed the mechanism underlying the effect of simvastatin-loaded silk fibroin. The results showed that the simvastatin-loaded silk fibroin membrane had better biocompatibility and the experiments confirmed that it could promote the proliferation and osteogenic differentiation of BMSCs. In addition, the HE staining experiments similarly confirmed that it could enhance tendon bone healing and alleviate inflammation in chronic rotator cuff injuries. On the other hand, Masson and Sirius staining showed that the simvastatin-loaded silk fibroin could promote the formation of collagen. Further analysis also revealed that it could promote the osteogenic differentiation of BMSCs by activating the β-catenin signaling pathway. In general, these findings suggested that surface modification of the simvastatin factor-loaded silk fibroin was a potential means of improving the healing of rotator cuff injuries and can be implemented in clinical practice in future.
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http://dx.doi.org/10.1177/0885328221995926DOI Listing
August 2021