Publications by authors named "Hao Jiang"

1,201 Publications

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Recent advances in sensitivity enhancement for lateral flow assay.

Mikrochim Acta 2021 10 13;188(11):379. Epub 2021 Oct 13.

Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.

Conventional lateral flow assay (LFA) is typically performed by observing the color changes in the test lines by naked eyes, which achieves considerable commercial success and has a significant impact on the fields of food safety, environment monitoring, disease diagnosis, and other applications. However, this qualitative detection method is not very suitable for low levels of disease biomarkers' detection. Although many nanomaterials are used as new labels for LFA, additional readers limit their application to some extent. Fortunately, a lot of work has been done for improving the sensitivity of LFA. In this review, currently reported LFA sensitivity enhancement methods with an objective evaluation are summarized, such as sample pretreatment, the change of flow rate, and label evolution, and future development direction and challenges of LFAs are discussed.
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http://dx.doi.org/10.1007/s00604-021-05037-zDOI Listing
October 2021

Optimal timing of combining sorafenib with trans-arterial chemoembolization in patients with hepatocellular carcinoma: A meta-analysis.

Transl Oncol 2021 Oct 7;14(12):101238. Epub 2021 Oct 7.

Department of Radiology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150086, China.

Background: The combination therapy of trans-arterial chemoembolization (TACE) and sorafenib were proved to be one of the effective methods for intermediate and advanced hepatocellular carcinoma (HCC). Although it has been confirmed that the combination therapy can prolong survival for advanced HCC effectively, the therapeutic efficacy and safety are still controversial and the clinical value has not been determined. This meta-analysis aims to evaluate the efficacy and safety of combination therapy and discuss the optimal timing of combination for better clinical benefits.

Data Sources: PubMed, EMBASE, the Cochrane Library, MEDLINE, and Web of Science were systematically reviewed to search for relevant studies published before May 15, 2021. Studies comparing the efficacy and safety of TACE + sorafenib with TACE + placebo / alone were adopted. Two reviewers independently extracted study outcomes. The data were analyzed through fixed/random-effect meta-analysis models with Review Manager (Version 5. 3) software.

Results: 7 randomized controlled trials (RCTs) were included with 1464 patients with unresectable HCC (734 in TACE + sorafenib group and 730 in TACE + placebo or alone group). Meta-analysis showed that objective response rate (ORR) and disease control rate (DCR) were slightly improved in TACE + sorafenib group (ORR: risk ratio = 1.24; 95% confidence interval: 1.08-1.42; P = 0.002; DCR: risk ratio = 1.09; 95% confidence interval: 1.01-1.18; P = 0.02). The combination therapy obviously improved time to progression (TTP) (hazard ratio: 0.73; 95% confidence interval: 0.55-0.96; P = 0.03) and progression-free survival (PFS) (hazard ratio 0.62; 95% confidence interval: 0.52-0.73, P < 0.00001) but not overall survival (OS) (hazard ratio: 0.93; 95% confidence interval: 0.59-1.46; P = 0.75) or time to untreatable progression (TTUP) (hazard ratio: 0.76; 95% confidence interval: 0.31-1.89; P = 0.56). In addition, the incidence of adverse reactions (AEs) in combination group were higher than TACE + placebo / alone group. Furthermore, the subgroup analysis showed that the heterogeneity of TTP was notably decreased (pre-TACE: P = 0.12, I = 48%; post-TACE: P = 0.58, I = 0%), and the hazard ratio was 0.59 (95% confidence interval: 0.51-0.68; P < 0.00001) in pre-TACE subgroup which indicated that combination before TACE significantly prolonged TTP but not in combination after TACE (hazard ratio: 0.88; 95% confidence interval: 0.62-1.24; P = 0.46). In term of AEs, sensitivity analysis indicated that the risk ratio for hand-foot skin reaction, diarrhea, rash/desquamation, and hypertension was 7.41, 2.58, 2.14, 1.55 in pre-TACE subgroup respectively and was 11.34, 3.26, 3.61, 4.11 in post-TACE subgroup respectively (All P < 0.05).

Conclusion: The combination of TACE and sorafenib significantly can improve TTP and PFS, and reduce the level of risk of adverse reactions of unresectable HCC, especially in the combination before TACE.
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http://dx.doi.org/10.1016/j.tranon.2021.101238DOI Listing
October 2021

Light-Motivated SnO /TiO Heterojunctions Enabling the Breakthrough in Energy Density for Lithium-Ion Batteries.

Adv Mater 2021 Oct 8:e2103558. Epub 2021 Oct 8.

Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Materials Science and Engineering, East China University of Science & Technology, Shanghai, 200237, China.

Powering lithium-ion batteries (LIBs) by light-irradiation will bring a paradigm shift in energy-storage technologies. Herein, a photoaccelerated rechargeable LIB employing SnO /TiO heterojunction nanoarrays as a multifunctional anode is developed. The electron-hole pairs generated by the Li TiO (x ≥ 0) under light irradiation synergistically enhance the lithiation kinetics and electrochemical reversibility of both SnO and TiO . Specifically, the electrons can quickly pour into the SnO and the generated Sn due to the more positive conduction band potentials (vs TiO ), and mean while the holes also promote the intercalation of Li into TiO by reaching charge balance. A remarkable increase in areal specific capacity is therefore achieved from 1.91 to 3.47 mAh cm at 5 mA cm . More impressively, there is no capacity loss even through 100 cycles, which is the best report for photorechargeable LIBs to date, owing to the strong and stable photoresponse current. This finding exhibits a feasible pathway to break the limitation in the energy density of LIBs by the efficient conversion and storage of solar energy.
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http://dx.doi.org/10.1002/adma.202103558DOI Listing
October 2021

Preparing potato starch nanocrystals assisted by dielectric barrier discharge plasma and its multiscale structure, physicochemical and rheological properties.

Food Chem 2021 Sep 27;372:131240. Epub 2021 Sep 27.

College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address:

Non-thermal plasma has increasingly been used for surface modification of various materials as a novel green technology. In this study, we prepared potato starch nanocrystals (SNCs) assisted by dielectric barrier discharge plasma technology and investigated its multiscale structure, physicochemical properties and rheology. Plasma treatment did not change the morphology and crystalline pattern of SNCs but reduced the crystallinity. The amylose content, swelling power, gelatinization temperature, and apparent viscosity of SNCs decreased after the plasma process by depolymerizing the amylopectin branch chains and degrading SNCs molecules. Besides, plasma increased the rapidly digestible starch and resistant starch content. Changes in rheological properties of plasma treated SNCs suggested that the plasma process increased the flowing capacity. The effective structural and functional changes of plasma treated SNCs confirm that plasma technology has great potential for modification of SNCs.
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http://dx.doi.org/10.1016/j.foodchem.2021.131240DOI Listing
September 2021

Reconstruction of finite deep sub-wavelength nanostructures by Mueller-matrix scattered-field microscopy.

Opt Express 2021 Sep;29(20):32158-32168

Computational super-resolution is a novel approach to break the diffraction limit. The Mueller matrix, which contains full-polarization information about the morphology and structure of a sample, can add super-resolution information and be a promising way to further enhance the resolution. Here we proposed a new approach called Mueller-matrix scattered-field microscopy (MSM) that relies on a computational reconstruction strategy to quantitatively determine the geometrical parameters of finite deep sub-wavelength nanostructures. The MSM adopts a high numerical-aperture objective lens to collect a broad range of spatial frequencies of the scattered field of a sample in terms of Mueller-matrix images. A rigorous forward scattering model is established for MSM, which takes into account the vectorial nature of the scattered field when passing through the imaging system and the effect of defocus in the measurement process. The experimental results performed on a series of isolated Si lines have demonstrated that MSM can resolve a feature size of λ/16 with a sub-7 nm accuracy. The MSM is fast and has a great measurement accuracy for nanostructures, which is expected to have a great potential application for future nanotechnology and nanoelectronics manufacturing.
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http://dx.doi.org/10.1364/OE.432611DOI Listing
September 2021

Transparent and ultra-wideband metamaterial absorber using coupled hexagonal combined elements.

Opt Express 2021 Aug;29(18):29439-29448

Optically transparent microwave absorbers have been widely reported for electromagnetic stealth applications over the past few years, but developing ultra-wideband absorbers with high angular stability remains challenging. In this work, an absorber comprising a double-layer polymethylpentene (TPX) block and indium tin oxide (ITO) films has been designed, fabricated, and measured, respectively. Firstly, an impedance layer with novel coupled hexagonal combined elements is exploited to achieve ultra-wideband absorption. Secondly, to provide the optimal reflection response for high angular incidences, the TPX block with the lower permittivity is initially employed in the compensation and substrate layers. Finally, the experimental results agreed with simulation ones illustrate the excellent performance is concurrently achieved, including a 90% absorption bandwidth within 2.53-8.94 (111.8%), high angular stability (60°), and the high light transmittance (70.7%).
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http://dx.doi.org/10.1364/OE.434327DOI Listing
August 2021

Therapeutic Nanoparticles from Grape Seed for Modulating Oxidative Stress.

Small 2021 Oct 3:e2102485. Epub 2021 Oct 3.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China.

The therapeutic potential of nanomaterials toward oxidative damage relevant diseases has attracted great attentions by offering promising advantages compared with conventional antioxidants. Although different kinds of nanoantioxidants have been well developed, the facile fabrication of robust and efficient nanoscavengers is still met with challenges like the use of toxic and high-cost subunits, the involvement of multistep synthetic process, and redundant purification work. Herein, a direct fabrication strategy toward polyphenol nanoparticles with tunable size, excellent biocompatibility, and reactive oxygen species (ROS) scavenging capacities from grape seed via an enzymatic polymerization method is reported. The resulting nanoparticles can efficiently prevent cell damage from ROS and exert promising in vivo antioxidant therapeutic effects on several oxidative stress-related diseases, including accelerating wound healing, inhibiting ulcerative colitis, and regulating the oxidative stress in dry eye disease. This study can stimulate the development of more kinds of low-cost, safe, and efficient biomass-based antioxidative nanomaterials via similar fabrication methodologies.
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http://dx.doi.org/10.1002/smll.202102485DOI Listing
October 2021

Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH.

Animals (Basel) 2021 Aug 31;11(9). Epub 2021 Aug 31.

Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun 130062, China.

The pituitary gland, an important endocrine organ, can secrete a variety of reproductive hormones under the action of hypothalamus-secreted gonadotropin-releasing hormone (GnRH) and plays important roles in animal reproduction. Circular RNAs (circRNAs) are a class of RNA molecules with stable covalently closed circular structures. CircRNAs are equipped with miRNA response elements (MREs), which can regulate the expression of target genes by competitively binding miRNAs. However, whether the expression levels of circRNAs in the pituitary gland change under the action of GnRH and whether such changes can further affect the secretion of reproductive hormones are still unclear. In this study, we performed RNA sequencing (RNA-Seq) of GnRH-treated rats to identify differentially expressed circRNAs. The results revealed 1433 related circRNAs, 14 of which were differentially expressed. In addition, we randomly selected five differentially expressed circRNAs and tested their relative expression levels by RT-qPCR, the results of which were consistent with the RNA sequencing results. Finally, we predicted targeted relationships between the differentially expressed circRNAs and FSHb-LHb-associated miRNAs. In all, a total of 14 circRNAs were identified that may act on the secretion and regulation of reproductive hormones in GnRH-treated rats. Our expression profiles of circRNAs in the anterior pituitaries of rats treated with GnRH can provide insights into the roles of circRNAs in mammalian development and reproduction.
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http://dx.doi.org/10.3390/ani11092557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466137PMC
August 2021

Minimally invasive treatment of hepatic hemangioma by transcatheter arterial embolization combined with microwave ablation: A case report.

World J Clin Cases 2021 Aug;9(24):7154-7162

Department of Hepatobiliary Surgery, Taizhou Central Hospital (Affiliated Hospital of Taizhou University), Taizhou 510000, Zhejiang Province, China.

Background: Hepatic hemangioma is the most common benign tumor of the liver. However, patients with large hemangiomas that cause compression symptoms or that are at risk of rupture may need further intervention. It is necessary to explore additional minimally invasive and personalized treatment options for hemangiomas.

Case Summary: A 47-year-old woman was diagnosed with a right hepatic hemangioma for more than 10 years. Abdominal contrast-enhanced computed tomography (CT) and contrast-enhanced ultrasound revealed that there was a large hemangioma in the right liver, with a size of approximately 95 mm 97 mm 117 mm. Due to the patient's refusal of surgical treatment, hepatic artery embolization was performed in the first stage. After 25 d of liver protection treatment, the liver function indexes decreased to normal levels. Then, ultrasound-guided microwave ablation of the giant hepatic hemangioma was performed. Ten days after the treatment, hepatobiliary ultrasonography showed that the hemangioma of the right liver was smaller than the previous size (the volume was reduced by approximately 30%). Then the patient was discharged from the hospital. One year after discharge, CT showed that the hepatic hemangioma had shrunk by about 80.

Conclusion: Transcatheter arterial embolization combined with microwave ablation is a safe and effective minimally invasive treatment for hepatic hemangioma.
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http://dx.doi.org/10.12998/wjcc.v9.i24.7154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409202PMC
August 2021

Synergistic partial denitrification, anammox and in-situ fermentation (SPDAF) process for treating domestic and nitrate wastewater: Response of nitrogen removal performance to decreasing temperature.

Bioresour Technol 2021 Sep 1;342:125865. Epub 2021 Sep 1.

College of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China.

A synergistic partial denitrification, anaerobic ammonium oxidation (Anammox), and in-situ fermentation (SPDAF) system was established to solve problems of wastewater treatment plants (WWTPs) in combined treatment of domestic sewage, and nitrate wastewater discharged from industrial areas. The SPDAF system was started up at decreasing temperatures (26.8-18.9 ℃), and remained robust at abrupt temperature drop and drastic temperature fluctuations (20.7-14.1 ℃). The influent and effluent total inorganic nitrogen (TIN) were 97.0 ± 3.7 mg/L and 10.3 ± 4.0 mg/L, respectively. In-situ fermentation supplemented electron donors for NO-N reduction. A high TIN removal efficiency, of 89.5 ± 3.9% was obtained. Specifically, Anammox contributed 90.9 ± 5.2% to TIN removal. Furthermore, the abundances of hydrolysis and acidogenesis bacteria were 14.02% and 29.47% in the low and high zones, respectively, which promoted fermentation and the use of complex organics. This study provided novel insights for actual operation of WWTPs.
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http://dx.doi.org/10.1016/j.biortech.2021.125865DOI Listing
September 2021

Diagnostic and prognostic value of MATN3 expression in gastric carcinoma: TCGA database mining.

J Gastrointest Oncol 2021 Aug;12(4):1374-1383

Department of Intensive Care Unit, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Backgrounds: Globally, the high morbidity and mortality of gastric carcinoma (GC) have been one of the great challenges facing humanity. However, the early diagnosis of GC is still unknown. Matrilin-3 (MATN3) is a member of the extracellular matrix (ECM) protein family. Previous studies have reported a correlation between the expression of MATN3 and bone disease. However, the role of MATN3 in GC has not been reported in depth, which can have a possible far-reaching implication for GC.

Methods: We explored the diagnostic and prognostic value and pathway enrichment of MATN3 expression in GC. Limma package conducted by R was used to analysis the difference expression data of MATN3 from The Cancer Genome Atlas (TCGA). The receiver operating characteristic (ROC) curve analysis was used to estimate the diagnostic value of MATN3 expression. univariate and multivariate analysis were used to assess the prognostic value of MATN3, and gene set enrichment analysis (GSEA) to identify the enriched signaling pathways.

Results: MATN3 was found to be significantly higher in GC tissue samples. GC patients with high MATN3 expression had poor prognosis. Then, GSEA showed that the gene sets were correlated with signaling pathways including ECM receptor interaction, hypertrophic cardiomyopathy (HCM), and glycosaminoglycan biosynthesis chondroitin sulfate, among others.

Conclusions: The study suggests that MATN3 can serve as a potential diagnostic and prognostic biomarker for GC.
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http://dx.doi.org/10.21037/jgo-21-267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421911PMC
August 2021

UTX condensation underlies its tumour-suppressive activity.

Nature 2021 Sep 15;597(7878):726-731. Epub 2021 Sep 15.

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, USA.

UTX (also known as KDM6A) encodes a histone H3K27 demethylase and is an important tumour suppressor that is frequently mutated in human cancers. However, as the demethylase activity of UTX is often dispensable for mediating tumour suppression and developmental regulation, the underlying molecular activity of UTX remains unknown. Here we show that phase separation of UTX underlies its chromatin-regulatory activity in tumour suppression. A core intrinsically disordered region (cIDR) of UTX forms phase-separated liquid condensates, and cIDR loss caused by the most frequent cancer mutation of UTX is mainly responsible for abolishing tumour suppression. Deletion, mutagenesis and replacement assays of the intrinsically disordered region demonstrate a critical role of UTX condensation in tumour suppression and embryonic stem cell differentiation. As shown by reconstitution in vitro and engineered systems in cells, UTX recruits the histone methyltransferase MLL4 (also known as KMT2D) to the same condensates and enriches the H3K4 methylation activity of MLL4. Moreover, UTX regulates genome-wide histone modifications and high-order chromatin interactions in a condensation-dependent manner. We also found that UTY, the Y chromosome homologue of UTX with weaker tumour-suppressive activity, forms condensates with reduced molecular dynamics. These studies demonstrate a crucial biological function of liquid condensates with proper material states in enabling the tumour-suppressive activity of a chromatin regulator.
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http://dx.doi.org/10.1038/s41586-021-03903-7DOI Listing
September 2021

and Investigation of S1PR1 Expression in the Central Nervous System Using [H]CS1P1 and [C]CS1P1.

ACS Chem Neurosci 2021 Oct 13;12(19):3733-3744. Epub 2021 Sep 13.

Department of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, United States.

Sphingosine-1-phosphate receptor 1 (S1PR1) is ubiquitously expressed among all tissues and plays key roles in many physiological and cellular processes. In the central nervous system (CNS), S1PR1 is expressed in different types of cells including neurons, astrocytes, and oligodendrocyte precursor cells. S1PR1 has been recognized as a novel therapeutic target in multiple sclerosis and other diseases. We previously reported a promising S1PR1-specific radioligand, [C]CS1P1 (previously named [C]TZ3321), which is under clinical investigation for human use. In the current study, we performed a detailed characterization of [H]CS1P1 for its binding specificity to S1PR1 in CNS using autoradiography and immunohistochemistry in human and rat CNS tissues. Our data indicate that [H]CS1P1 binds to S1PR1 in human frontal cortex tissue with a of 3.98 nM and a of 172.5 nM. The distribution of [H]CS1P1 in human and rat CNS tissues is consistent with the distribution of S1PR1 detected by immunohistochemistry studies. Our microPET studies of [C]CS1P1 in a nonhuman primate (NHP) show a standardized uptake value of 2.4 in the NHP brain, with test-retest variability of 0.23% among six different NHPs. Radiometabolite analysis in the plasma samples of NHP and rat, as well as in rat brain samples, showed that [C]CS1P1 was stable . Kinetic modeling studies using a two-compartment tissue model showed that the positron emission tomography (PET) data fit the model well. Overall, our study provides a detailed characterization of [H]CS1P1 binding to S1PR1 in the CNS. Combined with our microPET studies in the NHP brain, our data suggest that [C]CS1P1 is a promising radioligand for PET imaging of S1PR1 in the CNS.
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http://dx.doi.org/10.1021/acschemneuro.1c00492DOI Listing
October 2021

The influence of water in electrodes on the solid electrolyte interphase film of micro lithium-ion batteries for the wireless headphone.

J Colloid Interface Sci 2021 Aug 24;606(Pt 2):1729-1736. Epub 2021 Aug 24.

School of Metallurgy and Environment, Central South University, Changsha 410083, PR China.

During the production of micro lithium-ion batteries (LIBs), which are widely used in wireless headphones and other small portable devices, numerous factors can affect their quality, among which the content of water plays a crucial role. In this work, the influence of water in electrodes on the performances of micro LIBs is studied deeply. When the content of water increases, both the rate performance and the cycling performance of the batteries fade. The discharge capacity retention of the battery from high water content sample group H (group H) is 81.81% after 350 cycles at 2C, while that of the battery from low water content sample group L (group L) is 89.89% under the same condition. As for the rate performance, the discharge capacity of group H is only 58.66% of group L at 5C. To take a step further, it is mainly because an overgrowth of the solid electrolyte interphase film happen with the growth of water content. Accordingly, excess lithium ions are consumed and the porous structure of the anode is destroyed. Considering the results above, we believe that this work can offer a theory foundation to carry out the failure analysis of micro batteries.
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http://dx.doi.org/10.1016/j.jcis.2021.08.137DOI Listing
August 2021

Risk Stratification of Cytogenetically Normal Acute Myeloid Leukemia With Biallelic Mutations Based on a Multi-Gene Panel and Nomogram Model.

Front Oncol 2021 17;11:706935. Epub 2021 Aug 17.

Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Peking University People's Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing, China.

Background: Approximately 30% of Chinese individuals with cytogenetically normal acute myeloid leukemia (CN-AML) have biallelic (bi) mutations. The prognosis and optimal therapy for these patients are controversial in clinical practice.

Methods: In this study, we performed targeted region sequencing of 236 genes in 158 individuals with this genotype and constructed a nomogram model based on leukemia-free survival (LFS). Patients were randomly assigned to a training cohort ( =111) and a validation cohort ( =47) at a ratio of 7:3. Risk stratification was performed by the prognostic factors to investigate the risk-adapted post-remission therapy by Kaplan-Meier method.

Results: At least 1 mutated gene other than was identified in patients and mutation number was associated with LFS (61.6% 39.0%, =0.033), survival (85.6% 62.9%,  =0.030) and cumulative incidence of relapse (CIR) (38.4% 59.5%, =0.0496). White blood cell count, mutations in , and DNA methylation related genes were weighted to construct a nomogram model and differentiate two risk subgroups. Regarding LFS, low-risk patients were superior to the high-risk (89.3% 33.8%, 0.001 in training cohort; 87.5% 18.2%, =0.009 in validation cohort). Compared with chemotherapy, allogenic hematopoietic stem cell transplantation (allo-HSCT) improved 5-year LFS (89.6% 32.6%, 0.001), survival (96.9% 63.6%, =0.001) and CIR (7.2% 65.8%, 0.001) in high-risk patients but not low-risk patients (LFS, 77.4% 88.9%, =0.424; survival, 83.9% 95.5%, =0.173; CIR, 11.7% 11.1%, =0.901).

Conclusions: Our study indicated that bi mutant-positive CN-AML patients could be further classified into two risk subgroups by four factors and allo-HSCT should be recommended for high-risk patients as post-remission therapy. These data will help physicians refine treatment decision-making in bi mutant-positive CN-AML patients.
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http://dx.doi.org/10.3389/fonc.2021.706935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415912PMC
August 2021

Schisanhenol improves early porcine embryo development by regulating the phosphorylation level of MAPK.

Theriogenology 2021 Nov 17;175:34-43. Epub 2021 Aug 17.

Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun, 130062, Jilin, China. Electronic address:

Schisanhenol (SAL), a biphenyl cyclooctene-type lignin compound which can be extracted and isolated from many plants of the Schisandra family, exhibits a variety of biological activities including anti chronic cough, night sweating, thirst, diabetes, and obesity. However, its effects on the female reproductive system are unclear. Previous studies showed that SAL had potential antioxidant activity in heart, liver, and brain. Therefore, we hypothesized that SAL could improve porcine early development by reducing oxidative stress. The purpose of this study was to investigate the effects of SAL on preimplantation porcine embryos and the potential mechanisms. In this study, we analyzed the effects of SAL on embryo quality, reactive oxygen species (ROS) accumulation, mitochondrial function, cell proliferation and apoptosis, and the activation of MAPK pathway. The results showed that 10 μM SAL significantly increased the blastocyst formation rate, proliferation ability, and mitochondrial activity while reducing ROS accumulation and apoptosis level. During this process, the phosphorylation levels of ERK1/2, JNK1/2/3, and p38 were decreased. In summary, 10 μM SAL improves porcine preimplantation embryo development by reducing ROS accumulation.
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http://dx.doi.org/10.1016/j.theriogenology.2021.08.019DOI Listing
November 2021

A nomogram prediction of pressure injury in critical ill patients: A retrospective cohort study.

Int Wound J 2021 Sep 3. Epub 2021 Sep 3.

Department of Blood Transfusion, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.

Pressure injury (PI) is still a significant public health problem to be solved. Accurate prediction can lead to timely prophylaxis and therapy. However, the currently used Braden score shows insufficient predictive validity. We aimed to develop a nomogram to predict PI development in critically ill patients. We extracted data from Medical Information Mart for Intensive Care-IV v1.0. Variable selection was based on univariate logistic regression and all-subset regression. The area under the receiver operating characteristic curve (AUC) was used to assess the performance of the nomogram and Braden score. Decision curve analysis (DCA) was performed to identify and compare the clinical usefulness between the nomogram model and Braden score. We have developed a novel and practical nomogram that accurately predicts pressure ulcers. The AUC of the new model was better than that of the Braden score (P < .001). DCA showed that the nomogram model had a better net benefit than the Braden score at any given threshold. This finding needs to be confirmed by external validation as well as multicentre prospective studies.
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http://dx.doi.org/10.1111/iwj.13680DOI Listing
September 2021

Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC.

Bio Protoc 2021 Aug 5;11(15):e4106. Epub 2021 Aug 5.

Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom.

Polysome profile analysis is a popular method for separating polysomes and ribosomal subunits and is typically achieved using a sucrose density gradient (SDG). This has remained the gold standard method since ribosomes were first discovered; however, this method is time-consuming and requires multiple steps from making the gradient and long ultracentrifugation to collecting and analyzing the fractions. Each of these steps in the SDG workflow can introduce potential technical variation that affects the reproducibility of gradient profiles between samples. To address these limitations, we have developed a flexible, alternative approach for analyzing polysomes and ribosomal subunits based on size-exclusion chromatography (SEC), termed 'Ribo Mega-SEC.' In comparison with the SDG method, Ribo Mega-SEC involves a single step using ultra-high-performance liquid chromatography (uHPLC). The entire workflow, from injecting the lysate to collecting the fractions, can be performed in as little as 15 min, with high reproducibility. By varying the pore size of the SEC column, polysomes and ribosomal subunits can be separated using extracts from either human or mouse cultured cell lines or from tissue samples, embryos, or budding yeast. The resulting separated fractions are suitable for analysis using a wide range of subsequent analytical techniques including mass spectrometry (MS)-based proteomics, RNA-Seq, electron microscopy (EM), and multiple biochemical assays.
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http://dx.doi.org/10.21769/BioProtoc.4106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376616PMC
August 2021

The F-box E3 ubiquitin ligase BAF1 mediates the degradation of the brassinosteroid-activated transcription factor BES1 through selective autophagy in Arabidopsis.

Plant Cell 2021 Aug 26. Epub 2021 Aug 26.

Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.

Brassinosteroids (BRs) regulate plant growth, development and stress responses by activating the core transcription factor BRI1-EMS-SUPPRESSOR1 (BES1), whose degradation occurs through the proteasome and autophagy pathways. The E3 ubiquitin ligase(s) that modify BES1 for autophagy-mediated degradation remain to be fully defined. Here, we identified an F-box family E3 ubiquitin ligase named BES1-ASSOCIATED F-BOX1 (BAF1) in Arabidopsis thaliana. BAF1 interacts with BES1 and mediates its ubiquitination and degradation. Our genetic data demonstrated that BAF1 inhibits BR signaling in a BES1-dependent manner. Moreover, BAF1 targets BES1 for autophagic degradation in a selective manner. BAF1-triggered selective autophagy of BES1 depends on the ubiquitin binding receptor DOMINANT SUPPRESSOR OF KAR2 (DSK2). Sucrose starvation-induced selective autophagy of BES1, but not bulk autophagy, was significantly compromised in baf1 mutant and BAF1-ΔF (BAF1 F-box decoy) overexpression plants, but clearly increased by BAF1 overexpression. The baf1 and BAF1-ΔF overexpression plants had increased BR-regulated growth but were sensitive to long-term sucrose starvation, while BAF1 overexpression plants had decreased BR-regulated growth but were highly tolerant of sucrose starvation. Our results not only established BAF1 as an E3 ubiquitin ligase that targets BES1 for degradation through selective autophagy pathway, but also revealed a mechanism for plants to reduce growth during sucrose starvation.
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http://dx.doi.org/10.1093/plcell/koab210DOI Listing
August 2021

Pullulanase modification of granular sweet potato starch: Assistant effect of dielectric barrier discharge plasma on multi-scale structure, physicochemical properties.

Carbohydr Polym 2021 Nov 24;272:118481. Epub 2021 Jul 24.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China. Electronic address:

This study explored the potential application of physical combined enzyme treatment to modify starch granules. Starch was modified by exposure to cold plasma (CP) for 1, 3, and 9 min and to pullulanase (PUL) for 12, 24, and 36 h. Individual treatments with CP and PUL somewhat modified starch structure and physicochemical properties. Nevertheless, compared with native starch and individual treatments, CP-PUL combined treatment significantly (p < 0.05) promoted the subsequent structural modification, increased the short-chain ratio and the amylose content, reduce the molecular weight and the relative crystallinity, and disturb the short-range order. CP also improved the properties of PUL-modified starch, including enhanced solubility, thermal properties and resistance to enzymatic hydrolysis but worsened swelling power and peak viscosity properties. This research provides a new perspective for the rational application of CP-PUL co-treated starch in the food industry.
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http://dx.doi.org/10.1016/j.carbpol.2021.118481DOI Listing
November 2021

Advanced applications of chemo-responsive dyes based odor imaging technology for fast sensing food quality and safety: A review.

Compr Rev Food Sci Food Saf 2021 09 18;20(5):5145-5172. Epub 2021 Aug 18.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, P. R. China.

Public attention to foodquality and safety has been increased significantly. Therefore, appropriate analytical tools are needed to analyze and sense the food quality and safety. Volatile organic compounds (VOCs) are important indicators for the quality and safety of food products. Odor imaging technology based on chemo-responsive dyes is one of the most promising methods for analysis of food products. This article reviews the sensing and imaging fundamentals of odor imaging technology based on chemo-responsive dyes. The aim is to give detailed outlines about the theory and principles of using odor imaging technology for VOCs detection, and to focus primarily on its applications in the field of quality and safety evaluation of food products, as well as its future applicability in modern food industries and research. The literatures presented in this review clearly demonstrated that imaging technology based on chemo-responsive dyes has the exciting effect to inspect such as quality assessment of cereal , wine and vinegar flavored foods , poultry meat, aquatic products, fruits and vegetables, and tea. It has the potential for the rapid, reliable, and inline assessment of food safety and quality by providing odor-image-basedmonitoring tool. Practical Application: The literatures presented in this review clearly demonstrated that imaging technology based on chemo-responsive dyes has the exciting effect to inspect such as quality assessment of cereal , wine and vinegar flavored foods, poultry meat, aquatic products, fruits and vegetables, and tea.
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http://dx.doi.org/10.1111/1541-4337.12823DOI Listing
September 2021

Prognostic significance of TIM-3 expression pattern at diagnosis in patients with t(8;21) acute myeloid leukemia.

Leuk Lymphoma 2021 Aug 18:1-10. Epub 2021 Aug 18.

Peking University People's Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing, China.

Acute myeloid leukemia (AML) with t(8;21) is a heterogeneous disease and needs to be stratified. Both, cancer cells and immune cells participate in tumor initiation, growth and progression and might affect clinical outcomes. TIM-3 (T cell immunoglobulin and mucin domain-containing protein 3), an immune checkpoint molecule, is expressed not only on immune cells but also on leukemic stem cells (LSCs) in AML. This prompted us to investigate the prognostic significance of TIM-3 in t(8;21) AML. A total of 47 t(8;21) AML patients were tested for TIM-3 expression by multi-parameter flow cytometry at diagnosis. 35 of these, who received chemotherapy alone or along with allogeneic hematopoietic stem cell transplantation were followed up. The expression pattern of TIM-3 on T-cells and NK (natural killer) cells as a whole (T + NK) and LSCs were evaluated independently. High percentage of T + NK - TIM-3 and CD34CD38TIM-3 cells were significantly associated with a high 2-year cumulative incidence of relapse (CIR) ( = 0.028, 0.016). Further, concurrent high frequencies of T + NK-TIM-3 and CD34CD38TIM-3 cells at diagnosis were significantly associated with a high 2-year CIR ( < 0.0001) and this together with c-KIT D816 mutation were the independent adverse prognostic factors for relapse (hazard ratio (HR)=2.5, [95% confidence interval (CI), 1.1-6.0],  = 0.04; HR = 46.5, [95% CI, 2.7-811.5],  = 0.009). In conclusion, the expression pattern of TIM-3 on both T and NK cells and LSCs at diagnosis had prognostic significance in t (8;21) AML.
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http://dx.doi.org/10.1080/10428194.2021.1966785DOI Listing
August 2021

PML-RARA transcript levels at the end of induction therapy are associated with prognosis in non-high-risk acute promyelocytic leukaemia with all-trans retinoic acid plus arsenic in front-line therapy: long-term follow-up of a single-centre cohort study.

Br J Haematol 2021 Aug 17. Epub 2021 Aug 17.

Peking University People's Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Research Unit of Key Technique for Diagnosis and Treatments of Hematologic Malignancies, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing, China.

Despite the high cure probability for acute promyelocytic leukaemia (APL), a minority of patients will relapse and the risk factors for relapse are unclear. We retrospectively analysed 212 patients who were diagnosed with non-high-risk APL and received all-trans retinoic acid (ATRA) plus arsenic as front-line therapy at Peking University Institute of Hematology from February 2014 to December 2018. A total of 176 patients (83%) received oral arsenic (realgar-indigo naturalis formula) plus ATRA, 36 patients (17%) received arsenic trioxide plus ATRA and 203 patients were evaluable for relapse. After a median (range) follow-up of 53·6 (24·3-85·4) months, two patients had molecular relapse and eight had haematological relapse. A promyelocytic leukaemia/retinoic acid receptor alpha (PML-RARA) transcript level of ≥6·5% at the end of induction therapy was associated with relapse (P = 0·031). The 5-year cumulative incidence of relapse, event-free survival and overall survival were 5·5%, 92·3% and 96·3% respectively. In conclusion, the present long-term follow-up study further confirmed the high cure probability of ATRA plus oral arsenic as front-line therapy for non-high-risk APL and showed that the PML-RARA transcript level at the end of induction therapy was associated with relapse.
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http://dx.doi.org/10.1111/bjh.17752DOI Listing
August 2021

Stable nitritation of mature landfill leachate via in-situ selective inhibition by free nitrous acid.

Bioresour Technol 2021 Nov 23;340:125647. Epub 2021 Jul 23.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address:

In-situ free nitrous acid (FNA) and free ammonia (FA) treatments are more feasible than side-stream methods to achieve nitritation. To assess the optimum conditions and long-term performance of in-situ inhibition by FNA, batch tests and a sequencing batch reactor (SBR) treating mature landfill leachate were conducted and established. As a result, the selective inhibition characteristic by FNA was more conspicuous than FA, and FNA (0.175 mg N/L, 6 h) treatment are more biocidal to nitrite oxidizing bacteria (NOB). Moreover, ammonia oxidizing bacteria (AOB) were more sensitive to the FA environment but its activity recovered preferentially compared to NOB. The SBR achieved a sustained nitrite accumulation rate above 90% for 200 days, with a significant decrease of NOB activity and microbial abundance according to qPCR and 16S rRNA gene sequencing results. In-situ selective inhibition by FNA (0.175 mg N/L, 6 h) has been proved to be effective to maintain stable nitritation.
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http://dx.doi.org/10.1016/j.biortech.2021.125647DOI Listing
November 2021

Anlotinib Combined with Cranial Radiotherapy for Non-Small Cell Lung Cancer Patients with Brain Metastasis: A Retrospectively, Control Study.

Cancer Manag Res 2021 4;13:6101-6111. Epub 2021 Aug 4.

The First Affiliated Hospital of Bengbu Medical College & Tumor Hospital Affiliated to Bengbu Medical College, Bengbu, 233004, People's Republic of China.

Introduction: Cranial radiotherapy (CRT) is the main treatment for non-small cell lung cancer (NSCLC) with brain metastasis (BM) and non-EGFR/ALK/ROS1-TKIs indication, and anlotinib can improve overall prognosis. However, the clinical effects of CRT combined with anlotinib for the treatment of NSCLC with BM remain unclear.

Methods: We retrospectively analyzed the clinical effects of anlotinib + CRT versus CRT alone in NSCLC patients with BM and non-EGFR/ALK/ROS1-TKIs indication from September 2016 to June 2020. The progression-free survival (PFS) and overall survival (OS) of anlotinib + CRT versus CRT alone were analyzed. After evaluation of the clinical characteristics to generate a baseline, the independent prognostic factors for intracranial PFS (iPFS) and OS were subjected to univariate and multivariate analysis. Finally, subgroup analysis for iPFS and OS was performed to assess treatment effects using randomized stratification factors and stratified Cox proportional hazards models.

Results: This study included data for 73 patients with BM at baseline. Of the 73 patients, 45 patients received CRT alone, and 28 patients received CRT + anlotinib. There was no significant difference in clinical features between the two groups ( > 0.05). Compared with the CRT group, the combined group had longer iPFS (median iPFS [miPFS]: 3.0 months vs 11.0 months, = 0.048). However, there were no significant differences in OS, extracranial PFS, and systemic PFS. For clinical features, univariate and multivariate analysis showed that the plus anlotinib treatment was an independent advantage predictor of iPFS (hazard ratio [HR] 0.51; 95% confidence interval [CI] 0.27-0.95; = 0.04), and age ≥57 years (HR 1.04, 95% CI 1.01-1.08, = 0.014) and KPS score ≤80 (HR 1.04, 95% CI 1.01-1.08, = 0.014) were independent disadvantage predictors of OS ( < 0.05). In addition, although this difference was not statistically significant ( > 0.05), the patients with the anlotinib + local CRT (LCRT) treatment had the longest iPFS (miPFS: 27.0 months) and OS (median OS [mOS]: 36 months). The miPFS and mOS values for the LCRT group were 11 months and 18 months, respectively, with shorter values for whole-brain RT (WBRT) + anlotinib group, WBRT + LCRT + anlotinib group, WBRT, and WBRT + LCRT.

Conclusion: Anlotinib can improve the intracranial lesion control and survival prognosis of NSCLC patients with CRT.
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http://dx.doi.org/10.2147/CMAR.S319650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349551PMC
August 2021

Discovery of High-Affinity Inhibitors of the BPTF Bromodomain.

J Med Chem 2021 Aug 10;64(16):12075-12088. Epub 2021 Aug 10.

School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.

The dysfunctional bromodomain PHD finger transcription factor (BPTF) exerts a pivotal influence in the occurrence and development of many human diseases, particularly cancers. Herein, through the structural decomposition of the reported BPTF inhibitor TP-238, the effective structural fragments were synthetically modified to obtain our lead compound DC-BPi-03. DC-BPi-03 was identified as a novel BPTF-BRD inhibitor with a moderate potency (IC = 698.3 ± 21.0 nM). A structure-guided structure-activity relationship exploration gave rise to two BPTF inhibitors with much higher affinities, DC-BPi-07 and DC-BPi-11. Notably, DC-BPi-07 and DC-BPi-11 show selectivities 100-fold higher than those of other BRD targets. The cocrystal structures of BPTF in complex with DC-BPi-07 and DC-BPi-11 demonstrate the rationale of chemical efforts from the atomic level. Further study showed that DC-BPi-11 significantly inhibited leukemia cell proliferation.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00721DOI Listing
August 2021

Structural Variations of the 3D Genome Architecture in Cervical Cancer Development.

Front Cell Dev Biol 2021 23;9:706375. Epub 2021 Jul 23.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.

Human papillomavirus (HPV) integration is the major contributor to cervical cancer (CC) development by inducing structural variations (SVs) in the human genome. SVs are directly associated with the three-dimensional (3D) genome structure leading to cancer development. The detection of SVs is not a trivial task, and several genome-wide techniques have greatly helped in the identification of SVs in the cancerous genome. However, in cervical cancer, precise prediction of SVs mainly translocations and their effects on 3D-genome and gene expression still need to be explored. Here, we have used high-throughput chromosome conformation capture (Hi-C) data of cervical cancer to detect the SVs, especially the translocations, and validated it through whole-genome sequencing (WGS) data. We found that the cervical cancer 3D-genome architecture rearranges itself as compared to that in the normal tissue, and 24% of the total genome switches their A/B compartments. Moreover, translocation detection from Hi-C data showed the presence of high-resolution (4;7) (q13.1; q31.32) and (1;16) (q21.2; q22.1) translocations, which disrupted the expression of the genes located at and nearby positions. Enrichment analysis suggested that the disrupted genes were mainly involved in controlling cervical cancer-related pathways. In summary, we detect the novel SVs through Hi-C data and unfold the association among genome-reorganization, translocations, and gene expression regulation. The results help understand the underlying pathogenicity mechanism of SVs in cervical cancer development and identify the targeted therapeutics against cervical cancer.
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http://dx.doi.org/10.3389/fcell.2021.706375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344058PMC
July 2021

Oral etoposide combined with oral arsenic plus retinoic acid for two cases with newly diagnosed high-risk acute promyelocytic leukemia during COVID19 pandemic.

Leuk Res Rep 2021 14;16:100258. Epub 2021 Jul 14.

Peking University Institute of Hematology, Peking University People's Hospital, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing, China.

cute promyelocytic leukemia (APL) is a highly curable hematology malignancy. The major factor influence prognosis of APL is early deaths (ED) during the course of induction therapy, especially in high-risk APL. Therefore, effective reduction of white blood cells and correction of coagulation abnormalities are the key points of treatment for high-risk APL. Due to COVID19 pandemic in China since Jan 2020, some patients with hematologic malignancies suspected of COVID-19 infection had been isolated and traditional intravenous chemotherapy drugs is not available in isolated wards. We had explored a regimen of an oral etoposide to reduce the tumor burden for high-risk APL and dual induction with retinoic acid (ATRA) and oral arsenic realgar-Indigo nautralis formula (RIF), and finally two cases of high-risk APL patients received complete remission in one month. It is indicated that pure oral induction regimen: oral etoposide, ATRA and RIF provides a novel therapy in outpatient clinics.
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http://dx.doi.org/10.1016/j.lrr.2021.100258DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326808PMC
July 2021

Microstructure and Mechanical Properties of Al-Mg-Si Similar Alloy Laminates Produced by Accumulative Roll Bonding.

Materials (Basel) 2021 Jul 27;14(15). Epub 2021 Jul 27.

Key Laboratory of Automotive Materials Ministry of Education, School of Material Science and Technology, Jilin University, Changchun 130022, China.

As the applications of heterogeneous materials expand, aluminum laminates of similar materials have attracted much attention due to their greater bonding strength and easier recycling. In this work, an alloy design strategy was developed based on accumulative roll bonding (ARB) to produce laminates from similar materials. Twin roll casting (TRC) sheets of the same composition but different cooling rates were used as the starting materials, and they were roll bonded up to three cycles at varying temperatures. EBSD showed that the two TRC sheets deformed in distinct ways during ARB processes at 300 °C. Major recrystallizations were significant after the first cycle on the thin sheet and after the third cycle on the thick sheet. The sheets were subject to subsequent aging for better mechanical properties. TEM observations showed that the size and distribution of nano-precipitations were different between the two sheet sides. These nano-precipitations were found to significantly promote precipitation strengthening, and such a promotive effect was referred to as hetero-deformation induced (HDI) strengthening. Our work provides a new promising method to prepare laminated heterogeneous materials with similar alloy TRC sheets.
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http://dx.doi.org/10.3390/ma14154200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348428PMC
July 2021

Synthesis of hydroindoles via desymmetric [3+2] cycloadditions of para-quinamines with photogenerated ketenes.

Chem Commun (Camb) 2021 Sep 5;57(68):8496-8499. Epub 2021 Aug 5.

CCNU-uOttawa Joint Research Centre, Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, Hubei 430079, China.

A DBU-catalyzed desymmetric [3+2] cycloaddition between para-quinamines and photogenerated ketenes was developed for the first time. Under the irradiation of low-energy blue LEDs, a variety of hydroindoles bearing all-carbon quaternary centers were produced with good reaction efficiency and complete diastereoselectivity (34 examples, 45-99% yields and >95 : 5 dr). This protocol represents a new approach to synthetically significant hydroindoles, and features broad substrate scope, high functional group compatibility and mild reaction conditions.
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http://dx.doi.org/10.1039/d1cc03352bDOI Listing
September 2021
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