Publications by authors named "Hao He"

359 Publications

Formed Li-Ag Alloy Interface Enables LiGePS-Based All-Solid-State Lithium Batteries.

ACS Appl Mater Interfaces 2021 Oct 14. Epub 2021 Oct 14.

Key Laboratory of Material Physics, Ministry of Education, School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450001, P. R. China.

All-solid-state lithium-metal batteries (ASSLMBs) have received great interest due to their high potential to display both high energy density and safety performance. However, the poor compatibility at the Li/solid electrolyte (SE) interface and penetration of lithium dendrites during cycling strongly impede their successful commercialization. Herein, a thin Ag layer was introduced between Li and LiGePS for the formation of a Li-Ag alloy interface, thus tuning the interfacial chemistry and lithium deposition/dissolution behavior. Superior electrochemical properties and improved interfacial stability were achieved by optimizing the Ag thicknesses. The assembled symmetric cell with [email protected] 1 μm showed a steady voltage evolution up to 1000 h with an areal capacity of 1 mAh cm. Moreover, a high reversible capacity of 106.5 mAh g was achieved in an all-solid-state cell after 100 cycles, demonstrating the validity of the Ag layer. This work highlights the importance of the Li/SE interface re-engineering and provides a new strategy for improving the cycle life of ASSLMBs.
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http://dx.doi.org/10.1021/acsami.1c16356DOI Listing
October 2021

PmiREN2.0: from data annotation to functional exploration of plant microRNAs.

Nucleic Acids Res 2021 Sep 23. Epub 2021 Sep 23.

Beijing Agro-biotechnology Research Center, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R. China.

Nearly 200 plant genomes have been sequenced over the last two years, and new functions of plant microRNAs (miRNAs) have been revealed. Therefore, timely update of the plant miRNA databases by incorporating miRNAs from the newly sequenced species and functional information is required to provide useful resources for advancing plant miRNA research. Here we report the update of PmiREN2.0 (https://pmiren.com/) with an addition of 19 363 miRNA entries from 91 plants, doubling the amount of data in the original version. Meanwhile, abundant regulatory information centred on miRNAs was added, including predicted upstream transcription factors through binding motifs scanning and elaborate annotation of miRNA targets. As an example, a genome-wide regulatory network centred on miRNAs was constructed for Arabidopsis. Furthermore, phylogenetic trees of conserved miRNA families were built to expand the understanding of miRNA evolution across the plant lineages. These data are helpful to deduce the regulatory relationships concerning miRNA functions in diverse plants. Beside the new data, a suite of design tools was incorporated to facilitate experimental practice. Finally, a forum named 'PmiREN Community' was added for discussion and resource and new discovery sharing. With these upgrades, PmiREN2.0 should serve the community better and accelerate miRNA research in plants.
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http://dx.doi.org/10.1093/nar/gkab811DOI Listing
September 2021

TP53 and CDKN2A mutations in patients with early-stage lung squamous cell carcinoma: an analysis of the correlations and prognostic outcomes.

Ann Transl Med 2021 Aug;9(16):1330

Department of Thoracic Oncology Surgery, Fujian Medical University Cancer Hospital, Fujian Cancer Hospital, Fuzhou, China.

Background: Lung squamous cell carcinoma (LUSC) is characterized by frequent mutations of tumor protein p53 (TP53) and cyclin dependent kinase inhibitor 2A (CDKN2A). However, to date, the impact of TP53/CDKN2A status on the clinical outcome of patients with early-stage LUSC is unclear.

Methods: Tissue samples from 16 early-stage, surgically resected LUSCs were analyzed by next-generation sequencing (NGS). Information regarding TP53 and CDKN2A alterations and patient survival time was downloaded from The Cancer Genome Atlas (TCGA) database. The associations between TP53 and CDKN2A status and tumor characteristics, outcomes including overall survival (OS) and disease-free survival (DFS), and mutation counts were investigated.

Results: TP53 and CDKN2A exhibited a high frequency of somatic mutations in early-stage LUSC in our center. Data for 1,176 samples were collected from TCGA. CDKN2A mutation status was associated with TP53 mutation status (P=0.040). TP53 mutation was a favorable prognostic factor for early-stage LUSC. The OS times of patients with wild-type and mutated TP53 were 28.94 and 60.48 months, respectively (P=0.002). In contrast, CDKN2A mutations were significantly associated with a shorter survival time in early-stage LUSC. The OS times for wild-type and mutated CDKN2A patients were 62.81 and 37.55 months, respectively (P=0.026). Patients with TP53 mutations had higher total mutation counts compared to patients with wild-type TP53. Furthermore, OS was significantly shorter in patients with a low mutation count compared to patients with a median or high mutation count.

Conclusions: Early-stage LUSC patients with TP53 mutations had a longer OS, while those with CDKN2A mutations had a shorter OS. Furthermore, patients with TP53 mutation/CDKN2A wild-type status had a longer OS. CDKN2A mutation is a vital indicator for prognostic assessment according to TP53 status. The prolonged survival of patients with TP53 mutations may be due to their high mutation counts. Larger datasets are required to validate these observations.
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http://dx.doi.org/10.21037/atm-21-3709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422115PMC
August 2021

Clinical value of lymph node dissection for stage T1b mid-thoracic esophageal squamous cell carcinoma.

J Gastrointest Oncol 2021 Aug;12(4):1223-1227

Fujian Medical University Cancer Hospital, Fujian Cancer Hospital, Fujian Provincial Key Laboratory of Tumor Biotherapy, Department of Thoracic Tumor Surgery, Fujian Cancer Hospital, Fuzhou, China.

Background: Surgery remains the mainstay of treatment for esophageal squamous cell carcinoma (ESCC), during which lymph node (LN) dissection, especially recurrent laryngeal nerve (RLN) LN dissection, is particularly important and challenging. This study aimed to investigate the LN metastasis of stage T1b mid-thoracic ESCC and explore the clinical value of RLN LN dissection.

Methods: The clinicopathological data of 254 patients with stage T1b mid-thoracic ESCC who underwent the McKeown procedure ("tri-incisional esophagectomy") and three-field LN dissection (3FD) at Fujian Cancer Hospital from January 2010 to December 2015 were retrospectively analyzed. The value of LN dissection (especially RLN LNs) was evaluated by calculating the metastasis rate of each LN station. The efficacy index (EI) of the dissection was calculated by multiplying the frequency (%) of metastases to a station and the 5-year survival rate (%) of patients with metastases to that station, and then dividing by 100.

Results: The stage T1b mid-thoracic ESCC had the highest rate of metastasis in the paracardiac LNs (4.3%), followed by RLN LNs (2.8%) and the left gastric artery LNs (2.8%). The 5-year survival rate was highest in patients who received lesser gastric curvature LN dissection (100%), followed by patients who underwent right RLN LN dissection (80%), and was 50% in patients who had undergone dissection of the left RLN LNs, upper paraesophageal LNs, subcarinal LNs, and left gastric artery LNs, respectively. In addition, dissection of the right RLN LNs had the highest EI value (2.2), followed by the dissection of LNs along the lesser curvature of the stomach (1.6) and left gastric artery LNs (1.4).

Conclusions: Right RLN LNs have a metastasis rate only lower than that of the paracardiac LNs, but could be the most valuable location for performing dissection.
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http://dx.doi.org/10.21037/jgo-21-339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421909PMC
August 2021

Reply to Comment on "In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells".

Light Sci Appl 2021 Sep 17;10(1):189. Epub 2021 Sep 17.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.

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http://dx.doi.org/10.1038/s41377-021-00625-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446013PMC
September 2021

Analysis of anatomical variations of the lingular artery of the left upper lobe using 3D computed tomography angiography and bronchography.

J Thorac Dis 2021 Aug;13(8):5035-5041

Department of Thoracic Surgery, Fujian Medical University Cancer Hospital, Fujian Cancer Hospital, Fuzhou, China.

Background: Thoracic surgeons must be familiar with the anatomy of the pulmonary artery during segmentectomy and segmentectomy. But pulmonary arteries have numerous variations and aberrant branching patterns. The purpose of the present study was to analyze the anatomical variations and frequencies of the lingular artery of the left upper lobe (LUL) using 3D computed tomography angiography and bronchography (3D-CTAB).

Methods: We retrospectively studied 166 patients having undergone lobectomy or segmentectomy from January to December 2020 at Fujian Medical University Cancer Hospital's Department of Thoracic Surgery. All patients underwent 3D reconstruction using 3D-CTAB before surgery.

Results: The lingular segment was supplied by 1 artery in 45.18% of cases, 2 arteries in 46.39% of cases, and 3 arteries in 8.43% of cases. The branching patterns of the lingular artery included 119 (71.68%) cases with interlobar origin, 35 (21.08%) cases with interlobar and mediastinal origin, and 13 (7.83%) cases with mediastinal origin. The interlobar lingular artery include superior lingular artery (A4) and inferior lingular artery (A5). The interlobar lingular artery type was A4a, A4b, A5 in 7.23% of cases; A4 and A4b+5 in 3.01% of cases; and A4b and A4a+5 in 4.82% of cases. The mediastinal lingular artery was divided into the following 5 types: 'A4', 'A4b', 'A4b+5', 'A4b+5a', and 'A4+5'. The most common type was A4 (12.05%, 20/166) in 166 patients. The interlobar lingular artery had the following 5 patterns of variation: 'A4+5', 'A4, A5', 'A4a, A4b, A5', 'A4a, A4b+5', and 'A4b, A4a+5'. The single interlobar lingular artery (A4+5) was the most common type in 38.55% of cases. In 24.10% of cases, A5 came from A8 or A8+9. Besides In 8.43% of cases, the origin of A5 was close to A8 or A8+9.

Conclusions: We identified the left various lingular artery branching patterns with 3D-CTAB in patients and defined the frequency of anatomic variations. 3D-CTAB is useful for finding these variations.
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http://dx.doi.org/10.21037/jtd-21-1141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411136PMC
August 2021

Short-term and intermediate-term readmission after esophagectomy.

J Thorac Dis 2021 Aug;13(8):4678-4689

Division of Thoracic Surgery, Department of Cardiothoracic Surgery, Stanford University Medical Center, Falk Building, Stanford, CA, USA.

Background: The objective of this study was to characterize short- and intermediate-term readmissions following esophagectomy and to identify predictors of readmission in these two groups.

Methods: Patients who underwent esophagectomy in the National Readmissions Database (2013-2014) were grouped according to whether first readmission was "short-term" (readmitted <30 days) or "intermediate-term" (readmitted 31-90 days) following index admission for esophagectomy. Predictors of readmission were evaluated using multivariable logistic regression modeling.

Results: Of the 3,005 patients who underwent esophagectomy, 544 (18.1%) had a short-term readmission and 305 (10.1%) had an intermediate-term readmission. The most frequent reasons for short-term readmission were post-operative infection (7.5%), dysphagia (6.3%) and pneumonia (5.1%). The most common intermediate-term complications were pneumonia (7.2%), gastrointestinal stricture/stenosis (6.9%) and dysphagia (5.9%). In multivariable analysis, being located in a micropolitan area, increasing number of comorbidities and higher severity of illness score were associated with an increased likelihood of having a short-term readmission while being discharged to a facility (as opposed to directly home) was associated with increased likelihood of both short- and intermediate-term readmission (all P<0.05).

Conclusions: In this analysis, postoperative infection was the most common reason for short-term readmission. Dysphagia and pneumonia were common reasons for both short- and intermediate-term readmission of patients following esophagectomy. Interventions focused on reducing the risk of postoperative infection and pneumonia may reduce hospital readmissions. Gastrointestinal stricture and dysphagia were associated with increased risk of intermediate readmission and should be examined in the context of morbidity associated with pyloric procedures (e.g., pyloromyotomy) at the time of esophagectomy.
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http://dx.doi.org/10.21037/jtd-21-637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411130PMC
August 2021

Early Results of a Novel Gutter-Free Chimney Stent-Graft System to Treat Aortic Arch Dissection: Single-Center Data from a Prospective Clinical Trial.

J Endovasc Ther 2021 Sep 14:15266028211045699. Epub 2021 Sep 14.

Department of Vascular Surgery, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Aims: Discuss the clinical value, technique characteristics, and early follow-up results of a newly designed gutter-free chimney stent-graft system for aortic arch pathology.

Methods And Results: About 13 patients with aortic arch dissection were enrolled in a clinical trial testing a novel gutter-free stent-graft between February 2019 and December 2020. All 13 patients were male, age 52.6±10.4 years. The implantation time was 14.0±6.9 minutes; total procedure time was 89.5±19.8 minutes. The volume of contrast was 79.6±7.2 ml. And 15 aortic stent-grafts were implanted, and all 13 patients had chimney branch stent-grafts implanted into the left subclavian artery (LSA). There were 3 (23.1%) cases of immediate type Ιa endoleak after thoracic endovascular aortic repair (TEVAR), and 7.7% type Ιa endoleaks occurred in delayed fashion. Survival at 2 years was 100%, and the 2-year patency of chimney stent-grafts was 100%.

Conclusions: This study reports early success with good freedom from endoleak using a novel stent-graft designed for chimney TEVAR to treat aortic arch dissection. Postoperative survival and patency of the branch stent-grafts were excellent. Additional data from this multicenter clinical trial will be forthcoming.
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http://dx.doi.org/10.1177/15266028211045699DOI Listing
September 2021

Self-Radiopaque Markers Guiding Physician-Modified Fenestration (S-Fenestration) in Aortic Arch Endovascular Repair.

Front Cardiovasc Med 2021 20;8:713301. Epub 2021 Aug 20.

Department of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.

Thoracic endovascular aortic repair (TEVAR) has currently become the "first-line choice" for descending aortic pathologies. For pathologies located at the aortic arch, TEVAR with physician-modified fenestration (PMF) has been gained popularity as an alternative choice. However, stent fenestration is an experience-dependent technique and comes with possible adverse events such as misalignment. This study aims to introduce the self-radiopaque PMF (SF), which uses the radiopaque marker as a guiding indicator. This is a single-center retrospective study of 125 patients who underwent the SF-TEVAR in Second Xiangya Hospital from December 2015 to December 2020. Data include basic clinical information and technique records of SF-TEVAR with follow-up results. According to the SF-TEVAR protocol, we have performed the procedures on 125 patients and obtained an instant success rate of 98.4%. A total of 140 aortic stent-grafts and 44 bridging stents have been implanted in this study. The operation time is 64.6 ± 19.3 min, X-ray exposure time (from first digital subtraction angiography (DSA) to last DSA) is 25.6 ± 14.3 min, and contrast volume is 82.2 ± 22.6 ml. The success rate of PMF alignment is 98.4%. One bailout stent-graft was implanted into the left subclavian artery (LSA) by the chimney technique (0.8%). One fenestration was successfully and immediately corrected after misalignment (0.8%). Large simultaneous fenestration was performed in six patients (4.8%) for the left common carotid artery (LCCA) and LSA and in two patients (1.6%) for IA, LCCA, and LSA. One hundred twenty-two out of 125 patients' LSAs have been kept patent by the technique during the follow-up. The bridging stent group consists of 44 patients who received LSA stents, while the non-bridging stent group includes the other 81 patients. Type I endoleak has occurred in seven patients (5.6%) 1 week after the procedure. During follow-up (23 ± 18 months), survival rate is 95.7% and branch artery patent rate is 97.4%. The SF-TEVAR technique, which utilizes the radiopaque marker in stent-graft as an indication for PMF in TEVAR, seems a likely safe, effective, and efficient procedure that brings acceptable survival rate and branch artery patency rate. SF-TEVAR serves as a progressive alternative method to keep the branch artery patent in aortic arch endovascular reconstruction.
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http://dx.doi.org/10.3389/fcvm.2021.713301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417741PMC
August 2021

Aortic Geometric Alteration Associated With Acute Type B Aortic Dissection: Angulation, Tortuosity, and Arch Type.

Front Physiol 2021 20;12:708651. Epub 2021 Aug 20.

Department of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.

Acute type B aortic dissection is a highly serious aortic pathology. Aortic geometric parameters may be useful variables related to the occurrence of acute type B aortic dissection (aTBAD). The aim of the study is to delineate the alteration in aortic geometric parameters and analyze the specific geometric factors associated with aTBAD. The propensity score matching method was applied to control confounding factors. The aortic diameter, length, angulation, tortuosity, and type of aortic arch of the aTBAD and control group were retrospectively analyzed three-dimensional computed tomography imaging created by the 3mensio software (version 10.0, Maastricht, The Netherlands). The geometric variables of true lumen and false lumen in the descending aorta were measured to estimate the severity of aortic dissection. Multivariable logistic regression models were used to investigate the significant and specific factors associated with aTBAD occurrence. The area under the receiver operating characteristic curve (AUC) was used to estimate the performance of the model. After propensity score matching, 168 matched pairs of patients were selected. The ascending aorta and aortic arch diameters were dilated, and the ascending aorta and total aorta lengths were elongated in aTBAD group significantly ( < 0.001). The ascending aorta and aortic arch angulations in the aTBAD group were sharper than those of the controls ( = 0.01, < 0.001, respectively). The aortic arch and total aorta tortuosities were significantly higher in the aTBAD group ( = 0.001, < 0.001, respectively). There were more type III arch patients in the aTBAD group than the controls (67.9 vs. 22.6%). The true lumen angulation was sharper than that in the false lumen ( < 0.01). The true lumen tortuosity was significantly lower than that in the false lumen ( < 0.001). The multivariable models identified that aortic arch angulation, tortuosity, and type III arch were independent and specific geometric factors associated with aTBAD occurrence. The AUC of the multivariable models 1, 2, 3 were 0.945, 0.953, and 0.96, respectively. The sharper angulation and higher tortuosity of aortic arch and type III arch were the geometric factors associated with aTBAD in addition to the ascending aorta elongation and aortic arch dilation. The angulation and tortuosity of the true and false lumens may carry significant clinical implications for the treatment and prognosis of aTBAD.
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http://dx.doi.org/10.3389/fphys.2021.708651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417830PMC
August 2021

Beyond information rate, the capacity of cognitive control predicts response criteria in perceptual decision-making.

Brain Cogn 2021 Sep 1;154:105788. Epub 2021 Sep 1.

Center for Brain Disorders and Cognitive Sciences, Shenzhen University, Shenzhen, China; Shenzhen-Hong Kong Institute of Brain Science, Shenzhen, China. Electronic address:

Recent studies indicate that higher capacity of cognitive control (CCC) represents higher processing efficiency (i.e., high accuracy with fast speed). However, the speed-accuracy tradeoff (SAT) exists ubiquitously in decision-making, and little is known about whether and how the CCC is associated with SAT and whether the CCC-SAT relationship would be affected by changes in information entropy. In this study, fifty-nine college students performed a majority function task in which accuracy and response speed were equally emphasized. A Bayesian-based hierarchical drift diffusion modeling method was used to estimate three parameters of boundary separation, drift rate, and nondecision time for each participant in this task. In addition, the CCC of each participant was estimated. The results showed that the CCC was positively correlated with the SAT represented by jointly increasing accuracy and reaction time (RT), which was modulated by the change in task-relevant information entropy. Multiple mediation analyses indicated that drift rate served as the key mediator in the positive CCC-accuracy relationship while boundary separation played the major mediating role in the positive CCC-RT relationship. These findings suggest that the CCC reflects not only the rate of information processing but also decision strategies for achieving current goals.
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http://dx.doi.org/10.1016/j.bandc.2021.105788DOI Listing
September 2021

Porcelain aorta associated with longstanding Takayasu arteritis.

Eur Heart J Cardiovasc Imaging 2021 Sep 4. Epub 2021 Sep 4.

Department of Vascular Surgery, Second Xiangya Hospital, Central South University, 139 Middle Renmin Road, Changsha 410011, China.

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http://dx.doi.org/10.1093/ehjci/jeab176DOI Listing
September 2021

Decline in the integration of top-down and bottom-up attentional control in older adults with mild cognitive impairment.

Neuropsychologia 2021 10 31;161:108014. Epub 2021 Aug 31.

Center for Brain Disorders and Cognitive Sciences, Shenzhen University, Shenzhen, China; Center for Neuroimaging, Shenzhen Institute of Neuroscience, Shenzhen, China. Electronic address:

Individuals with mild cognitive impairment (MCI) have deficits in goal-directed top-down and stimulus-driven bottom-up attentional control. However, it remains unclear whether and how the interaction between the two processes is altered in individuals with MCI. We collected electroencephalography (EEG) data from 30 older adults with MCI and 30 demographically matched healthy controls (HCs) when they were performing a perceptual decision-making task, in which we manipulated the cognitive load involved in task-relevant top-down processing and the surprise level involved in task-irrelevant bottom-up processing. We found the significant group difference in the interaction between top-down and bottom-up processes. HCs showed enlarged P3 and strengthened event-related microstate C on high (vs. low) surprise level trials under high cognitive load, while there was no such surprise effect suggesting distraction under low cognitive load. In contrast, participants with MCI showed increased P2 and P3 amplitudes and strengthened microstates C and D on high (vs. low) surprise level trials under low cognitive load yet no surprise effect under high load. These results suggested that participants with MCI were distracted by task-irrelevant information under low cognitive load, while under high load, they might experience a passive inhibition on the task-irrelevant bottom-up processing because of the exhaustion of attentional resources; in addition, this altered interaction observed in the MCI group occurred at the stages of selective attention and uncertainty reduction.
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http://dx.doi.org/10.1016/j.neuropsychologia.2021.108014DOI Listing
October 2021

Emission characteristics of dioxins during iron ore Co-sintering with municipal solid waste incinerator fly ash in a sintering pot.

Chemosphere 2021 Aug 17;287(Pt 1):131884. Epub 2021 Aug 17.

Baowu Group Environmental Resources Technology Co. Ltd., Shanghai, 201900, PR China.

The disposing of municipal solid waste incineration(MSWI) fly ashes containing dioxins is an intractable problems. Co-sintering is one of the most ideal methods to dispose MSWI fly ash, because it not only degrades the dioxins but also makes it possible to re-utilize MSWI fly ashes. In the present study, MSWI fly ash(FA) and water washed MSWI fly ash(WFA) were added into the sinter raw mixture in a lab-scale sintering pot. Different effects of fly ash and water washed fly ash on emission characteristics of dioxins were studied, and possible pathways to form dioxins were discussed in detail. During co-sintering, at least 88.9%, 99.1% of dioxins brought in by FA, WFA was decomposed, respectively, and re-synthesis with a significant distribution characteristic of dioxins originated from sintering process. In the preheating and dry zone, the recombination and condensation reaction of precursors were the main formation pathways of 2,3,7,8-PCDDs in the sintering process and 2,3,7,8-PCDFs were formed by de novo synthesis. In addition, the resynthesis process was affected by chlorine and calcium brought in by fly ash. Sufficient chlorine boosted the chlorination of reactants while calcium increased the residence time, causing the emission concentration of toxic dioxins and the adding ratio were nonlinearly dependent. Therefore, the maximum proportion of water washed MSWI fly ash that can be added into the sintering process was 0.5 wt.%. Besides, the possible pathways to form dioxins were proposed.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131884DOI Listing
August 2021

Comparative effectiveness of endovascular treatment modalities for de novo femoropopliteal lesions in intermittent claudication: A network meta-analysis of randomized controlled trials.

Int J Cardiol 2021 Nov 27;343:122-130. Epub 2021 Aug 27.

Department of Vascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China; Department of Vascular Surgery, Fuwai Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, China. Electronic address:

Purpose: To evaluate the most effective endovascular treatment modalities for de novo femoropopliteal lesions in intermittent claudication (IC) in terms of technical success, primary patency, target lesion revascularization (TLR) and all-cause mortality through network meta-analysis of randomized controlled trials.

Methods: Medical databases were searched on December 3, 2020. 16 studies (3265 patients) and 7 treatments were selected. Outcomes were technical success, primary patency, TLR and mortality at 6 and/or 12 months.

Results: Regarding 6-month primary patency, drug-eluting stents (DES) was better than balloon angioplasty (BA; odds ratio [OR], 23.27; 95% confidence interval [CI], 12.57-43.06), drug-coated balloons (DCB; OR, 5.63; 95% CI, 2.26-14.03) and directional atherectomy (DA; OR, 31.52; 95% CI, 7.81-127.28), and bare nitinol stents (BNS) was better than BA (OR, 17.91; 95% CI, 7.22-44.48), DCB (OR, 4.33; 95% CI, 1.40-13.45) and DA (OR, 24.27; 95% CI, 5.16-114.11). Regarding 12-month primary patency, DES was better than BA (OR, 10.05; 95% CI, 4.56-22.16), DCB (OR, 3.70; 95% CI, 1.54-8.89) and DA (OR, 29.54; 95% CI, 7.26-120.26). DCB and combination of balloon and atherectomy were the most effective treatment regarding 12-month TLR and technical success (residual stenosis <30%), respectively. DES, BNS and DA with DCB (DA-DCB) were included in the best cluster in the clustered ranking plot combining 12-month primary patency and TLR.

Conclusions: Balloon and atherectomy may confer advantages over other treatments for technical success; DCB may for TLR. Stent technologies confer substantial advantages regarding primary patency. Stent technologies and DA-DCB should be given priority in treating femoropopliteal lesions in IC.
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http://dx.doi.org/10.1016/j.ijcard.2021.08.038DOI Listing
November 2021

Genetic Features of Plasmid- and Chromosome-Mediated in Isolates From Animal Organs With Lesions.

Front Microbiol 2021 5;12:707332. Epub 2021 Aug 5.

College of Veterinary Medicine, Northwest A&F University, Yangling, China.

The genomic context of the gene in from animal feces has been widely reported. However, less is known about the -carrying plasmid characteristics and other functional regions of isolates from animal organs with lesions. The present study investigated the antimicrobial resistance, population structure, and genetic features of -positive strains isolated from animal organs with lesions. The antimicrobial susceptibility testing indicated that 24 -positive isolates were resistant to at least three or all antimicrobial categories. MLST analysis suggested that the dominant clone complexes (CC) were mainly CC156, CC448, and CC10. In addition, ST10596, a newly discovered sequence type in swine, failed to be classified. Meanwhile, the gene located on the different plasmids was successfully transferred to the recipients, and whole-genome sequencing indicated the gene was embedded in cassette but not flanked by IS. The gene is located on the chromosome and embedded in Tn. Furthermore, was found on the IncX3-type plasmid of J-8. The and gene of type IV secretion system (T4SS) were truncated by IS and IS and located on the IncX4- and the IncHI2/HI2A/N-type plasmids, respectively. The multidrug-resistant (MDR) region of IncHI2/HI2A/N-type plasmids contained two class 1 integrons (In, In) and four composite transposons (Tn, Tn, cn_4692_IS, cn_6354_IS). Overall, 24 -positive isolates in our study showed MDR, or even extensively drug resistant (XDR), and exhibited population diversity. The T4SS gene truncation by the insertion sequence may affect the efficiency of plasmid conjugative transfer. Furthermore, the class 1 integrons and composite transposons in the MDR region of IncHI2/HI2A/n-type plasmid contributed to the multireplicon plasmid formation, the acquisition, and transfer of antimicrobial resistance genes (ARGs).
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http://dx.doi.org/10.3389/fmicb.2021.707332DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386294PMC
August 2021

Endovascular repair for retrograde type A intramural hematoma with focal intimal disruption in descending aorta.

J Thorac Dis 2021 Jul;13(7):4250-4259

Department of Vascular Surgery, the Second Xiangya Hospital, Central South University, Changsha, China.

Background: This study aims to report the experience of a single center using thoracic endovascular aortic repair (TEVAR) to treat retrograde type A intramural hematoma (IMH) with focal intimal disruption (FID) in descending aorta.

Methods: A total of 24 consecutive patients with retrograde type A IMH and complicated with FID in descending aorta underwent TEVAR in our center from 2015 to 2020. Their clinical data, imaging manifestation and follow-up results were retrospectively reviewed and analyzed.

Results: The median age of patients was 57.9 years (range, 42-80 years) and 18 were men (75%). As the preoperative CT angiography showed, the 24 patients developed IMH complicated with different kinds of FIDs in descending aorta [5 had intramural blood pool (IBP), 15 had ulcer-like projection (ULP), 2 had penetrating atherosclerotic ulcer (PAU), and 5 had localized dissection]. Successful deployment of aortic stent graft was achieved in all patients. There was no endoleak, stent graft migration, spinal cord ischemia, stroke, or 30-day mortality observed after TEVAR. The median duration of follow-up was 30.0 months (range, 3-60 months). As the last follow-up CT angiography showed, most of the patients (23 in 24, 96%) had favorable aortic remodeling. The maximum hematoma thicknesses and maximum diameters of both ascending and descending aorta were significantly decreased. During follow-up, 1 patient developed retrograde type A aortic dissection (RAAD) and underwent open surgery 3 months after TEVAR. 1 patient died of lung cancer 2 years later. There was no aorta-related death observed.

Conclusions: TEVAR provides a safe and effective treatment strategy for selected patients with retrograde type A IMH, and FID developed in descending aorta could be the possible treatment target. However, RAAD remains one of the most serious postoperative complications of concern.
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http://dx.doi.org/10.21037/jtd-21-574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339735PMC
July 2021

Paraffin-Enabled Compressive Folding of Two-Dimensional Materials with Controllable Broadening of the Electronic Band Gap.

ACS Appl Mater Interfaces 2021 Sep 19;13(34):40922-40931. Epub 2021 Aug 19.

Beijing Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China.

The capability to manipulate the size of the electronic band gap is of importance to semiconductor technology. Among these, a wide direct band gap is particularly helpful in optoelectronic devices due to the efficient utilization of blue and ultraviolet light. Here, we reported a paraffin-enabled compressive folding (PCF) strategy to widen the band gap of two-dimensional (2D) materials. Due to the large thermal expansion coefficient of paraffin, folded 2D materials can be achieved via thermal engineering of the paraffin-assisted transfer process. It can controllably introduce 0.2-1.3% compressive strain onto folded structures depending on the temperature differences and transfer the folding product to both rigid and soft substrates. Exemplified by MoS, its folded multilayers demonstrated blue-shifts at direct gap transition peaks, six times stronger photoluminescence intensity, almost double mobility, and 20 times higher photoresponsivity over unfolded MoS. This PCF strategy can attain controllable widening band gap of 2D materials, which will open up novel applications in optoelectronics.
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http://dx.doi.org/10.1021/acsami.1c11269DOI Listing
September 2021

Prediction of MoRFs based on sequence properties and convolutional neural networks.

BioData Min 2021 Aug 14;14(1):39. Epub 2021 Aug 14.

School of Electronic and Information Engineering, Hebei University of Technology, Tianjin, China.

Background: Intrinsically disordered proteins possess flexible 3-D structures, which makes them play an important role in a variety of biological functions. Molecular recognition features (MoRFs) act as an important type of functional regions, which are located within longer intrinsically disordered regions and undergo disorder-to-order transitions upon binding their interaction partners.

Results: We develop a method, MoRF, to predict MoRFs based on sequence properties and convolutional neural networks (CNNs). The sequence properties contain structural and physicochemical properties which are used to describe the differences between MoRFs and non-MoRFs. Especially, to highlight the correlation between the target residue and adjacent residues, three windows are selected to preprocess the selected properties. After that, these calculated properties are combined into the feature matrix to predict MoRFs through the constructed CNN. Comparing with other existing methods, MoRF obtains better performance.

Conclusions: MoRF is a new individual MoRFs prediction method which just uses protein sequence properties without evolutionary information. The simulation results show that MoRF is effective and competitive.
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http://dx.doi.org/10.1186/s13040-021-00275-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364704PMC
August 2021

Optimized adaptive Savitzky-Golay filtering algorithm based on deep learning network for absorption spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Dec 16;263:120187. Epub 2021 Jul 16.

Information Materials and Intelligent Sensing Laboratory of Anhui Province, Anhui University, 230601 Hefei, China; Key Laboratory of Opto-Electronic Information Acquisition and Manipulation of Ministry of Education, Anhui University, 230601 Hefei, China. Electronic address:

An improved Savitzky-Golay (S-G) filtering algorithm was developed to denoise the absorption spectroscopy of nitrogen oxide (NO). A deep learning (DL) network was introduced to the traditional S-G filtering algorithm to adjust the window size and polynomial order in real time. The self-adjusting and follow-up actions of DL network can effectively solve the blindness of selecting the input filter parameters in digital signal processing. The developed adaptive S-G filter algorithm is compared with the multi-signal averaging filtering (MAF) algorithm to demonstrate its performance. The optimized S-G filtering algorithm is used to detect NO in a mid-quantum-cascade-laser (QCL) based gas sensor system. A sensitivity enhancement factor of 5 is obtained, indicating that the newly developed algorithm can generate a high-quality gas absorption spectrum for applications such as atmospheric environmental monitoring and exhaled breath detection.
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http://dx.doi.org/10.1016/j.saa.2021.120187DOI Listing
December 2021

Synthesis and biological evaluations of oleanolic acid indole derivatives as hyaluronidase inhibitors with enhanced skin permeability.

J Enzyme Inhib Med Chem 2021 Dec;36(1):1665-1678

School of Biotechnology and Health Sciences, International Healthcare Innovation Institute (Jiangmen), Wuyi University, Jiangmen, China.

Oleanolic acid (OA) is a natural cosmeceutical compound with various skin beneficial activities including inhibitory effect on hyaluronidase but the anti-hyaluronidase activity and mechanisms of action of its synthetic analogues remain unclear. Herein, a series of OA derivatives were synthesised and evaluated for their inhibitory effects on hyaluronidase. Compared to OA, an induction of fluorinated () and chlorinated () indole moieties led to enhanced anti-hyaluronidase activity (IC = 80.3 vs. 9.97 and 9.57 µg/mL, respectively). Furthermore, spectroscopic and computational studies revealed that and can bind to hyaluronidase protein and alter its secondary structure leading to reduced enzyme activity. In addition, OA indole derivatives showed feasible skin permeability in a slightly acidic environment (pH = 6.5) and exerted skin protective effect by reducing cellular reactive oxygen species in human skin keratinocytes. Findings from the current study support that OA indole derivatives are potential cosmeceuticals with anti-hyaluronidase activity.
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http://dx.doi.org/10.1080/14756366.2021.1956487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317927PMC
December 2021

Morphological Features of Aortic Arch Predicting the Risk for Acute Type B Aortic Dissection.

World J Surg 2021 Nov 23;45(11):3458-3466. Epub 2021 Jul 23.

Department of Vascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410011, China.

Background: The purpose of the study is to elucidate the changes in aortic morphological parameters and identify the specific geometric risk factors associated with aTBAD.

Methods: A total of 167 patients in aTBAD group and 196 subjects in control group were enrolled in the study. The aortic morphological data were retrospectively analyzed by 3mensio Vascular software. The aortic morphological parameters include diameter, length, angulation, tortuosity and the type of aortic arch. Multivariable logistic regression models were developed to identify the significant predictors associated with the angulation, tortuosity and aortic arch type. The predictive capacity of the models was evaluated through the receiver operating characteristic (ROC) curve analysis.

Results: The diameters in the ascending aorta and aortic arch and the lengths in the ascending aorta and total aorta were significantly greater in aTBAD group. Besides, the angulation of the ascending aorta and aortic arch in aTBAD group increased significantly. The tortuosity of aortic arch and total aorta was significantly higher in aTBAD group. The prevalence of type III arch in aTBAD group was significantly higher than that in the controls. Multivariable models demonstrated that aortic arch angulation, tortuosity and type III arch were independent geometric predictors of aTBAD occurrence. Based on the ROC analysis, AUC of the risk prediction models was 0.977, 0.958 and 0.945, respectively.

Conclusions: Besides the enlargement of the ascending aorta and aortic arch, incremental aortic arch angulation, tortuosity and type III arch configuration are valuable geometric risk factors that could enhance aTBAD incidence.
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http://dx.doi.org/10.1007/s00268-021-06247-5DOI Listing
November 2021

Endovascular Reintervention 8 Years After Open Implantation of Triple-Branched Stent Graft.

Ann Thorac Surg 2021 10 14;112(4):e311. Epub 2021 Jul 14.

Department of Vascular Surgery, Second Xiangya Hospital, Central South University, Changsha, China; Center of Vascular Surgery, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

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http://dx.doi.org/10.1016/j.athoracsur.2021.06.029DOI Listing
October 2021

Influence of facility volume on long-term survival of patients undergoing esophagectomy for esophageal cancer.

J Thorac Cardiovasc Surg 2021 Jun 10. Epub 2021 Jun 10.

Department of Cardiothoracic Surgery, Falk Cardiovascular Research Institute, Stanford University Medical Center, Stanford, Calif; VA Palo Alto Health Care System, Palo Alto, Calif. Electronic address:

Objective: This study investigated the influence of facility volume on long-term survival in patients with esophageal cancer treated with esophagectomy.

Methods: Patients treated with esophagectomy for cT1 3N0 3M0 adenocarcinoma or squamous cell carcinoma of the mid-distal esophagus in the National Cancer Database between 2006 and 2013 were stratified by annual facility esophagectomy volume dichotomized as more/less than both 6 and 20. Patient characteristics associated with facility volume were evaluated using logistic regression, and the influence of facility volume on survival was evaluated with Kaplan-Meier curves, Cox proportional hazards methods, and propensity matched analysis.

Results: Of 11,739 patients who had esophagectomy at 1018 facilities where annual volume ranged from 1 to 47.6 cases, 4262 (36.3%) were treated at 44 facilities with annual esophagectomy volume > 6 and 1515 (12.9%) were treated at 7 facilities with annual volume > 20. Higher volume was associated with significantly better 5-year survival for both annual volume > 6 (47.6% vs 40.2%; P < .001) and annual volume > 20 (47.2% vs 42.3%; P < .001), which persisted in propensity matched analyses as well as Cox multivariable analysis (hazard ratio, 0.81; 95% confidence interval, 0.74-0.89; P < .001 for facility volume > 6 and hazard ratio, 0.78; 95% confidence interval, 0.65-0.95; P = .01 for facility volume > 20). In Cox multivariable analysis that considered facility volume as a continuous variable, higher volume continued to be associated with better survival (hazard ratio, 0.93 per 5 cases; 95% CI, 0.91-0.96; P < .001).

Conclusions: Esophageal cancer patients treated with esophagectomy at higher volume facilities have significantly better long-term survival than patients treated at lower volume facilities.
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http://dx.doi.org/10.1016/j.jtcvs.2021.05.048DOI Listing
June 2021

Corrigendum: CGRP Regulates the Age-Related Switch Between Osteoblast and Adipocyte Differentiation.

Front Cell Dev Biol 2021 22;9:715740. Epub 2021 Jun 22.

Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, China.

[This corrects the article DOI: 10.3389/fcell.2021.675503.].
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http://dx.doi.org/10.3389/fcell.2021.715740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259016PMC
June 2021

Mitochondrial Ca oscillation induces mitophagy initiation through the PINK1-Parkin pathway.

Cell Death Dis 2021 06 19;12(7):632. Epub 2021 Jun 19.

School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.

Dysregulation of the PINK1/Parkin-mediated mitophagy is essential to Parkinson's disease. Although important progress has been made in previous researches, the biochemical reagents that induce global and significant mitochondrial damage may still hinder deeper insights into the mechanisms of mitophagy. The origin of PINK1/Parkin pathway activation in mitophagy remains elusive. In this study, we develop an optical method, ultra-precise laser stimulation (UPLaS) that delivers a precise and noninvasive stimulation onto a submicron region in a single mitochondrial tubular structure. UPLaS excites localized mitochondrial Ca (mitoCa) oscillations with tiny perturbation to mitochondrial membrane potential (MMP) or mitochondrial reactive oxygen species. The UPLaS-induced mitoCa oscillations can directly induce PINK1 accumulation and Parkin recruitment on mitochondria. The Parkin recruitment by UPLaS requires PINK1. Our results provide a precise and noninvasive technology for research on mitophagy, which stimulates target mitochondria with little damage, and reveal mitoCa oscillation directly initiates the PINK1-Parkin pathway for mitophagy without MMP depolarization.
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http://dx.doi.org/10.1038/s41419-021-03913-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214625PMC
June 2021

CGRP Regulates the Age-Related Switch Between Osteoblast and Adipocyte Differentiation.

Front Cell Dev Biol 2021 26;9:675503. Epub 2021 May 26.

Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, China.

Osteoporosis is a chronic age-related disease. During aging, bone marrow-derived mesenchymal stem cells (BMSCs) display increased adipogenic, along with decreased osteogenic, differentiation capacity. The aim of the present study was to investigate the effect of calcitonin gene-related peptide (CGRP) on the osteogenic and adipogenic differentiation potential of BMSC-derived osteoblasts. Here, we found that the level of CGRP was markedly lower in bone marrow supernatant from aged mice compared with that in young mice. experiments indicated that CGRP promoted the osteogenic differentiation of BMSCs while inhibiting their adipogenic differentiation. Compared with vehicle-treated controls, aged mice treated with CGRP showed a substantial promotion of bone formation and a reduction in fat accumulation in the bone marrow. Similarly, we found that CGRP could significantly enhance bone formation in ovariectomized (OVX) mice . Together, our results suggested that CGRP may be a key regulator of the age-related switch between osteogenesis and adipogenesis in BMSCs and may represent a potential therapeutic strategy for the treatment of age-related bone loss.
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http://dx.doi.org/10.3389/fcell.2021.675503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187789PMC
May 2021

Early and Midterm Outcomes of Endovascular Repair of Tuberculous Infected Native (Mycotic) Aortic Aneurysms.

Eur J Vasc Endovasc Surg 2021 08 6;62(2):314-315. Epub 2021 Jun 6.

Department of Vascular Surgery, Second Xiangya Hospital, Central South University, Changsha, China; Department of Vascular Surgery, Fuwai Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China. Electronic address:

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http://dx.doi.org/10.1016/j.ejvs.2021.05.003DOI Listing
August 2021

p62 Overexpression Promotes Bone Metastasis of Lung Adenocarcinoma out of LC3-Dependent Autophagy.

Front Oncol 2021 21;11:609548. Epub 2021 May 21.

Department of Orthopaedics, Bone and Soft Tissue Tumors Research Center of Yunnan Province, The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming, China.

p62 protein has been implicated in bone metastasis and is a multifunctional adaptor protein usually correlated with autophagy. Herein, we investigated p62 expression and its prognostic significance in bone metastasis of lung adenocarcinoma, and analyzed whether the mechanism involved depends on autophagy. mRNA and protein expression of p62, LC3B and Beclin 1 were detected by reverse transcription-quantitative PCR and western blotting, respectively, in fresh bone metastasis tissues (n=6 cases) and normal cancellous bone tissues (n=3 cases). The association between p62 and LC3B expression and patient prognosis was subsequently analyzed in 62 paraffin-embedded bone metastasis specimens by immunohistochemistry assay. Small interfering RNA (siRNA) was employed to downregulate p62 expression in SPC-A-1 and A549 cells. Cell proliferation and migration ability were tested by CCK8, CCF and Transwell assays respectively. Autophagy was induced by Rapamycin or inhibited by Atg 7 knockout/Chloroquine in A549 cells and p62 and LC3II/I expression were analyzed. After subcutaneous inoculation or intracardial injection of A549 cells into nude mice, the effect of p62 downregulation was analyzed by histopathological examination. The results showed that p62, LC3B and Beclin 1 mRNA and protein were all overexpressed in bone metastasis tissues (all P<0.01). Patient samples with high p62 expression levels were significantly associated with more bone lesions (>3), shorter overall survival rates and shorter progression free survival rates compared with patients having lower p62 expression (P=0.014, P=0.003, P=0.048, respectively). Cox regression analysis identified p62 expression as an independent prognostic indicator of overall survival of patients with bone metastasis (P=0.007). p62 downregulation inhibited SPC-A-1 and A549 cells migration but had no effect on cell proliferation. After autophagy induction or inhibition, p62 expression involved in autophagy flux and changed inconsistently according to the switch of LC3I to LC3II in different autophagy conditions. p62 downregulation had no effect on growth of subcutaneous tumor. Lung or bone metastasis lesion was not found in all mice model. These findings suggested that p62 overexpression promotes tumor cell invasion out of LC3-dependent autophagy, which could be used a potential prognostic biomarker and therapeutic target for bone metastasis of lung adenocarcinoma.
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http://dx.doi.org/10.3389/fonc.2021.609548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175982PMC
May 2021

Single-Cell Transcriptomic Analysis Reveals a Hepatic Stellate Cell-Activation Roadmap and Myofibroblast Origin During Liver Fibrosis in Mice.

Hepatology 2021 Jun 5. Epub 2021 Jun 5.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.

Background And Aims: HSCs and portal fibroblasts (PFs) are the major sources of collagen-producing myofibroblasts during liver fibrosis, depending on different etiologies. However, the mechanisms by which their dynamic gene expression directs the transition from the quiescent to the activated state-as well as their contributions to fibrotic myofibroblasts-remain unclear. Here, we analyze the activation of HSCs and PFs in CCL -induced and bile duct ligation-induced fibrosis mouse models, using single-cell RNA sequencing and lineage tracing.

Approach And Results: We demonstrate that HSCs, rather than PFs, undergo dramatic transcriptomic changes, with the sequential activation of inflammatory, migrative, and extracellular matrix-producing programs. The data also reveal that HSCs are the exclusive source of myofibroblasts in CCL -treated liver, while PFs are the major source of myofibroblasts in early cholestatic liver fibrosis. Single-cell and lineage-tracing analysis also uncovers differential gene-expression features between HSCs and PFs; for example, nitric oxide receptor soluble guanylate cyclase is exclusively expressed in HSCs, but not in PFs. The soluble guanylate cyclase stimulator Riociguat potently reduced liver fibrosis in CCL -treated livers but showed no therapeutic efficacy in bile duct ligation livers.

Conclusions: This study provides a transcriptional roadmap for the activation of HSCs during liver fibrosis and yields comprehensive evidence that the differential transcriptomic features of HSCs and PFs, along with their relative contributions to liver fibrosis of different etiologies, should be considered in developing effective antifibrotic therapeutic strategies.
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http://dx.doi.org/10.1002/hep.31987DOI Listing
June 2021
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