Publications by authors named "Hao Guo"

670 Publications

Long-term outcome of stereotactic aspiration, endoscopic evacuation, and open craniotomy for the treatment of spontaneous basal ganglia hemorrhage: a propensity score study of 703 cases.

Ann Transl Med 2021 Aug;9(16):1289

Department of Neurosurgery, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.

Background: To compare the long-term therapeutic effects of stereotactic aspiration (SA), endoscopic evacuation (EE), and open craniotomy (OC) in the surgical treatment of spontaneous basal ganglia hemorrhage and explore the appropriate clinical indications for each technique.

Methods: Multiple-treatment inverse probability of treatment weighting (IPTW)-adjusted logistic regression analysis was performed to evaluate the therapeutic effects of these techniques. The primary and secondary outcomes were 6-month modified Rankin Scale (mRS) and mortality rates, respectively.

Results: A total of 703 patients were ultimately enrolled. For the entire cohort, the 6-month mortality rate was significantly higher (OR 2.396, 95% CI: 1.865-3.080), and the 6-month functional outcome was significantly worse (OR 1.359, 95% CI: 1.091-1.692) for SA than that of EE. The 6-month mortality rate for OC was significantly higher (OR 1.395, 95% CI: 1.059-1.837) than that of EE. Further subgroup analysis was stratified by initial hematoma volume and Glasgow Coma Scale (GCS) score. The mortality rate for SA was significantly higher for patients with hematoma volume of 20-40 mL (OR 6.226, 95% CI: 3.848-10.075), 40-80 mL (OR 2.121, 95% CI: 1.492-3.016), and ≥80 mL (OR 5.544, 95% CI: 3.315-9.269) than in the same subgroups of EE. The functional outcomes for SA were significantly worse than that of EE for hematoma volume subgroups of 40-80 mL (OR 1.424, 95% CI: 1.039-1.951) and ≥80 mL (OR 4.224, 95% CI: 1.655-10.776). The mortality rate for SA was significantly higher than that of EE for the GCS score subgroups of 6-8 (OR 2.082, 95% CI: 1.410-3.076) and 3-5 (OR 2.985, 95% CI: 1.904-4.678). The mortality rate for OC was significantly higher for the GCS score of 3-5 subgroup (OR 1.718, 95% CI: 1.115-2.648), and a tendency for a higher mortality rate of 6-8 subgroup (OR 1.442, 95% CI: 0.965-2.156) than that of EE.

Conclusions: EE can decrease the 6-month mortality rate and improve the 6-month functional outcomes of spontaneous basal ganglia hemorrhage in patients with a hematoma volume ≥40 mL. EE can decrease the 6-month mortality rate of spontaneous basal ganglia hemorrhage in patients with a GCS score of 3-8.
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http://dx.doi.org/10.21037/atm-21-1612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422088PMC
August 2021

Comprehensive identification of alterations in 5 557 Chinese patients with solid tumors by next-generation sequencing.

Am J Cancer Res 2021 15;11(8):3893-3906. Epub 2021 Aug 15.

Guangdong Lung Cancer Institute, Guangdong Provincial People's Hospital and Guangdong Academy of Medical Sciences Guangzhou 510080, Guandong, China.

Deregulation of fibroblast growth factor receptor (FGFR) network is common in cancer due to activating mutations, gene amplifications and chromosomal translocations. Currently, various FGFR inhibitors are being developed. In order to optimize their clinical applications, understanding the frequencies and types of alterations in multiple cancer types appears to be extremely important. This study characterized alterations in solid tumors by next-generation sequencing (NGS). Between Jun. 2019 and Aug. 2020, the sequencing data of 5 557 solid tumors of diverse types in the database of Simcere Diagnostics, Inc. (Nanjing, China) were retrospectively analyzed. A panel-based NGS assay was used to detect alterations in tumor samples. 9.2% of cancer cases had alterations, in which gene amplifications (51.5%) and mutations (40.7%) were frequent, whereas gene rearrangements were less common (10.0%). was involved in 4.6% of 5 557 cases, in 2.1%, in 1.6%, and in 1.4%. Of patients with alterations, , , , and genes were the top 5 mutant genes. aberrations occurred in almost every type of solid tumors, with the most common tumor being endometrial carcinoma (22.2%), followed by sarcoma (17.3%), breast cancer (13.2%), gastric cancer (12.2%), and more. 0.6% of cancer cases harbored fusions, with the most common fusion partner being . Two cases of GBM harboring fusions were responsive to anlotinib treatment. In conclusion, alterations are prevalent in solid tumors of diverse types, with the majority being gene amplifications and mutations. fusions only occur in a minority of cancer cases, and those with glioblastoma harboring fusions may benefit from anlotinib.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414391PMC
August 2021

Cryopreservation of Human T Lymphocytes under Fast Cooling with Controlled Ice Nucleation in Cryoprotective Solutions of Low Toxicity.

Cryobiology 2021 Sep 8. Epub 2021 Sep 8.

Institute of Biothermal and Technology, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Cryopreservation of human T lymphocytes has become an essential tool for some cell-based immunotherapy. However, the cryopreservation procedure of the cells has not been systematically studied. In particular, the key factors of ice seeding and cryoprotective agents (CPA) driving the success of cryopreservation remain unclear. We systematically investigated the key factors, including cooling rate, ice-seeding temperature, CPA concentration, and types of CPA, during cryopreservation of human T lymphocytes with controlled ice nucleation. We found that ice seeding at below -10 °C could enable human T lymphocytes to be cooled at 90 °C min with high relative viability and recovery rate after rewarming, 94.9% and 90.2%, respectively, which are significantly higher than those without ice seeding (P<0.001). After optimization, the concentration of dimethyl sulphoxide was as low as 2% (v/v) with relative viability and recovery rate of 95.4% and 100.8%, respectively, at the cooling rate of 90 °C min after ice seeding at -16 °C. The cryopreservation procedure developed in this study could facilitate the understanding of the mechanism for ice seeding and cell injury and offer a promising cryopreservation method with a high cooling rate and extremely low toxicity for extensive clinical application of immunotherapy.
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http://dx.doi.org/10.1016/j.cryobiol.2021.09.002DOI Listing
September 2021

Identification of Target PTEN-Based miR-425 and miR-576 as Potential Diagnostic and Immunotherapeutic Biomarkers of Colorectal Cancer With Liver Metastasis.

Front Oncol 2021 19;11:657984. Epub 2021 Aug 19.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.

A major complication of colorectal cancer (CRC), one of the most common and fatal types of cancers, is secondary liver metastasis. For patients with this fate, there are very few biomarkers available in clinical application, and the disease remains incurable. Recently, increasing studies demonstrated that tumorigenesis and development are closely related to immune escape, indicating that the roles of immune-related indicators might have been neglected in the past in colorectal cancer liver metastases (CRLM). Here, we unveil that elevated miR-425 and miR-576 promote CRLM through inhibiting PTEN-mediated cellular immune function. Specifically, miR-425 and miR-576 were identified for their significant upregulation in CRLM compared with the primary CRC tissues based on GSE81581 ( = 8) and GSE44121 ( = 18) datasets. Besides, we determined that the two microRNAs (miRNAs) coparticipated in restraining P53 and transforming growth factor beta (TGF-β) signaling pathways associated with tumor metastasis, and both shortened the overall survival of the patients with metastatic susceptibility. Notably, hybridization on relatively large samples of paired CRC tissues ( = 157) not only substantiated that the expression of miR-425 and miR-576 was dramatically upregulated in CRLM but also revealed that they were closely related to tumor deterioration, especially liver metastases. Moreover, we further confirmed that the combination of miR-425 and miR-576 was an effective predictive model for liver metastases and poor clinical outcomes. Mechanically, downregulated PTEN (GSE81558, = 6) was verified to be a shared target of miR-425 and miR-576 acting as metastasis-related oncogenes, on account of the presence of binding sites (+2928-+2934 and +4371-+4378, respectively) and the collaborative suppression of P53/TGF-β signaling in CRLM, which was further confirmed in CRC cells (HCT116 and SW480) based on systematic molecular biology experiments. Importantly, the target was strongly associated with microsatellite instability, tumor microenvironment, and immune cell infiltration. Thus, we speculate that miR-425 and miR-576 are novel biomarkers for CRLM prevention and immunotherapy and upstream inhibitors of the PTEN-P53/TGF-β function axis.
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http://dx.doi.org/10.3389/fonc.2021.657984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418231PMC
August 2021

Metal-Free Chemoselective Oxidation of 4-Methylquinolines into Quinoline-4-Carbaldehydes.

Chem Asian J 2021 Sep 2. Epub 2021 Sep 2.

Department of Chemistry, Fudan University, 2005 Songhu Road, Shanghai, 200438, P. R China.

A convenient protocol for the synthesis of quinoline-4-carbaldehydes via chemoselective oxidation of 4-methylquinolines using hypervalent iodine(III) reagents as oxidant is described. This method highlights metal-free and mild reaction conditions, nice yield, good functional group tolerance, and high chemoselectivity.
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http://dx.doi.org/10.1002/asia.202100704DOI Listing
September 2021

Design and analysis of a continuum robot for transnasal skull base surgery.

Int J Med Robot 2021 Aug 31:e2328. Epub 2021 Aug 31.

College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, China.

Background: The traditional surgical instruments for transnasal endoscopic skull base surgery have poor flexibility and continuum robot has attracted extensive attention for its high dexterity and safety.

Methods: A new type of micro continuum surgical robot is designed. On the basis of kinematic analysis, a superposition method is proposed to analyse the reachable configuration space of the system, and the workspace is analysed. The results obtained by superposition method can provide reference for the selection of joint parameters. At last, the static model of the system is established considering friction and coupling between two segments.

Results: The simulation results show that the workspace can meet the requirements of surgery. And the validity of the static model is verified by numerical simulation and experiment, which lays a foundation for the establishment of the driving force transmission system and precise control of the robot.

Conclusions: The research results of this study contribute to the real-time control and movement of robot. The proposed continuum robot provides convenient conditions for the clinical application.
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http://dx.doi.org/10.1002/rcs.2328DOI Listing
August 2021

Traitement de la kératose actinique de la lèvre inférieure par thérapie photodynamique.

CMAJ 2021 Aug;193(34):E1373-E1374

Département de dermatologie, premier hôpital de l'Université de médecine de Chine, Shenyang (Chine).

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http://dx.doi.org/10.1503/cmaj.202674-fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432312PMC
August 2021

Coastal eutrophication in China: Trend, sources, and ecological effects.

Harmful Algae 2021 Jul 13;107:102058. Epub 2021 Jun 13.

Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, PR China. Electronic address:

Eutrophication in coastal waters caused by excess nutrient inputs has occurred widely on a global scale. Due to the rapid economic development over the last four decades, most of the Chinese coastal waters have experienced a eutrophic process. Major observed trends of coastal eutrophication include two periods, a slow development from the 1970s to 1990s and a fast development after 2000, with major contributions of increased nitrogen (N) and phosphorus (P) from river inputs, atmospheric deposition, and submarine groundwater discharge (SGD). Nutrient composition and stoichiometry have been significantly changed, including increased ammonium, bioavailable organic N and P, and asymmetric ratios between N, P and silicate (Si). Most of these changes were related to the rapid increases in population density, fertilizer application, sewage discharge, aquaculture and fossil fuel combustion, and have resulted in distinctly increased harmful algal blooms. Coastal eutrophication combined with the effects of climate change is projected to continually grow in coming decades. Targeted research is therefore needed on nitrogen reduction and control, potential adaptation strategies and the consequences for ecosystems and economic sustainability.
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http://dx.doi.org/10.1016/j.hal.2021.102058DOI Listing
July 2021

SERS Gas Sensors Based on Multiple Polymer Films with High Design Flexibility for Gas Recognition.

Sensors (Basel) 2021 Aug 18;21(16). Epub 2021 Aug 18.

Graduate School and Faculty of Information Science and Electrical Engineering, Kyushu University, Fukuoka 819-0395, Japan.

The Surface-Enhanced Raman Scattering (SERS) technique is utilized to fabricate sensors for gas detection due to its rapid detection speed and high sensitivity. However, gases with similar molecular structures are difficult to directly discriminate using SERS gas sensors because there are characteristic peak overlaps in the Raman spectra. Here, we proposed a multiple SERS gas sensor matrix via a spin-coating functional polymer to enhance the gas recognition capability. Poly (acrylic acid) (PAA), Poly (methyl methacrylate) (PMMA) and Polydimethylsiloxane (PDMS) were employed to fabricate the polymer film. The high design flexibility of the two-layer film was realized by the layer-by-layer method with 2 one-layer films. The SERS gas sensor coated by different polymer films showed a distinct affinity to target gases. The principle component analysis (PCA) algorithm was used for the further clustering of gas molecules. Three target gases, phenethyl alcohol, acetophenone and anethole, were perfectly discriminated, as the characteristic variables in the response matrix constructed by the combination of gas responses obtained 3 one-layer and 3 two-layer film-coated sensors. This research provides a new SERS sensing approach for recognizing gases with similar molecular structures.
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http://dx.doi.org/10.3390/s21165546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402296PMC
August 2021

A Novel sEMG-Based Gait Phase-Kinematics-Coupled Predictor and Its Interaction With Exoskeletons.

Front Neurorobot 2021 10;15:704226. Epub 2021 Aug 10.

School of Computer Science and Technology, Harbin Institute of Technology, Harbin, China.

The interaction between human and exoskeletons increasingly relies on the precise decoding of human motion. One main issue of the current motion decoding algorithms is that seldom algorithms provide both discrete motion patterns (e.g., gait phases) and continuous motion parameters (e.g., kinematics). In this paper, we propose a novel algorithm that uses the surface electromyography (sEMG) signals that are generated prior to their corresponding motions to perform both gait phase recognition and lower-limb kinematics prediction. Particularly, we first propose an end-to-end architecture that uses the gait phase and EMG signals as the priori of the kinematics predictor. In so doing, the prediction of kinematics can be enhanced by the ahead-of-motion property of sEMG and quasi-periodicity of gait phases. Second, we propose to select the optimal muscle set and reduce the number of sensors according to the muscle effects in a gait cycle. Finally, we experimentally investigate how the assistance of exoskeletons can affect the motion intent predictor, and we propose a novel paradigm to make the predictor adapt to the change of data distribution caused by the exoskeleton assistance. The experiments on 10 subjects demonstrate the effectiveness of our algorithm and reveal the interaction between assistance and the kinematics predictor. This study would aid the design of exoskeleton-oriented motion-decoding and human-machine interaction methods.
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http://dx.doi.org/10.3389/fnbot.2021.704226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384035PMC
August 2021

Derivation of iPSC lines from two idiopathic ASD patients (OFi001-A, OFi002-A).

Stem Cell Res 2021 Aug 19;56:102510. Epub 2021 Aug 19.

Laboratory of Stem Cells and Organoids, OrganFactory Co., Ltd., Naesudong-ro, Seowon-gu, Cheongju-si, Chungcheongbuk-do 28864, Republic of Korea; Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Wuyi University, Jiangmen 529020, China. Electronic address:

Here we described two human induced pluripotent stem cell (hiPSC) lines from peripheral blood mononuclear cells (PBMCs) of idiopathic autism spectrum disorder (ASD) patients through forced expression of OCT4, SOX2, KLF4, and c-MYC. The hiPSC lines displayed morphology, gene expression patterns, and pluripotential differentiation potentials similar to those of human embryonic stem cells (hESCs). The hiPSC lines from idiopathic ASD patients might be useful to unveil the underlying mechanism of idiopathic ASD and finding its therapeutics.
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http://dx.doi.org/10.1016/j.scr.2021.102510DOI Listing
August 2021

Gli1 Cells Residing in Bone Sutures Respond to Mechanical Force via IPR to Mediate Osteogenesis.

Stem Cells Int 2021 12;2021:8138374. Epub 2021 Aug 12.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.

Early orthodontic correction of skeletal malocclusion takes advantage of mechanical force to stimulate unclosed suture remodeling and to promote bone reconstruction; however, the underlying mechanisms remain largely unclear. Gli1 cells in maxillofacial sutures have been shown to participate in maxillofacial bone development and damage repair. Nevertheless, it remains to be investigated whether these cells participate in mechanical force-induced bone remodeling during orthodontic treatment of skeletal malocclusion. In this study, rapid maxillary expansion (RME) mouse models and mechanical stretch loading cell models were established using two types of transgenic mice which are able to label Gli1 cells, and we found that Gli1 cells participated in mechanical force-induced osteogenesis both in vivo and in vitro. Besides, we found mechanical force-induced osteogenesis through inositol 1,4,5-trisphosphate receptor (IPR), and we observed for the first time that inhibition of Gli1 suppressed an increase in mechanical force-induced IP3R overexpression, suggesting that Gli1 cells participate in mechanical force-induced osteogenesis through IPR. Taken together, this study is the first to demonstrate that Gli1 cells in maxillofacial sutures are involved in mechanical force-induced bone formation through IPR during orthodontic treatment of skeletal malocclusion. Furthermore, our results provide novel insights regarding the mechanism of orthodontic treatments of skeletal malocclusion.
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http://dx.doi.org/10.1155/2021/8138374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8380501PMC
August 2021

[Network Meta-analysis of clinical efficacy of Chinese herbal injection in adjuvant treatment of unstable angina pectoris].

Zhongguo Zhong Yao Za Zhi 2021 Jul;46(13):3429-3445

Institute of Basic Medicine,Xiyuan Hospital,Chinese Academy of Chinese Medical Sciences Beijing 100091,China National Clinical Research Center of Cardiovascular Disease of Traditional Chinese Medicine Beijing 100091,China.

The present study evaluated the curative efficacy of Chinese herbal injection on unstable angina pectoris( UAP) by network Meta-analysis. The databases,including Pub Med,Cochrane Library,Web of Science,CNKI,CBM,VIP and Wanfang were searched for randomized controlled trial( RCT) of Chinese herbal injection in the treatment of UAP. All researchers independently screened the articles,extracted the data and evaluated the quality. Open BUGS and Stata were employed for the analysis of the trials that met the quality standards. Fifty-eight studies were finally included in this study,involving 20 intervention measures. In terms of the effective rate,16 injections such as Dengzhan Xixin Injection,Xuesaitong Injection and Danshen Injection combined with western medicine exhibited significant efficacy. In terms of ECG,Puerarin Injection,Ginkgo Leaf Extract and Dipyridamole Injection( GDI),Breviscapine Injection combined with western medicine were superior to western medicine. In terms of the reduction of the angina attack times,Sodium Tanshinone ⅡASulfonate Injection,GDI and Dazhu Hongjingtian Injection combined with western medicine showed better effects than western medicine. In terms of shortening the angina duration,Shenmai Injection combined with western medicine was superior to western medicine. As revealed by the results,Dengzhan Xixin Injection,Xuesaitong Injection,Danshen Injection,Breviscapine Injection,Danshen Ligustrazine Injection combined with western medicine displayed prominent curative efficacy,which were recommended for clinical application. Meanwhile,appropriate intervention measures should be selected according to individual conditions. Limited by the quality of the included trials,the conclusions still need to be further verified.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210506.501DOI Listing
July 2021

Characterization of Shallow Whole-Metagenome Shotgun Sequencing as a High-Accuracy and Low-Cost Method by Complicated Mock Microbiomes.

Front Microbiol 2021 30;12:678319. Epub 2021 Jul 30.

Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Characterization of the bacterial composition and functional repertoires of microbiome samples is the most common application of metagenomics. Although deep whole-metagenome shotgun sequencing (WMS) provides high taxonomic resolution, it is generally cost-prohibitive for large longitudinal investigations. Until now, 16S rRNA gene amplicon sequencing (16S) has been the most widely used approach and usually cooperates with WMS to achieve cost-efficiency. However, the accuracy of 16S results and its consistency with WMS data have not been fully elaborated, especially by complicated microbiomes with defined compositional information. Here, we constructed two complex artificial microbiomes, which comprised more than 60 human gut bacterial species with even or varied abundance. Utilizing real fecal samples and mock communities, we provided solid evidence demonstrating that 16S results were of poor consistency with WMS data, and its accuracy was not satisfactory. In contrast, shallow whole-metagenome shotgun sequencing (shallow WMS, S-WMS) with a sequencing depth of 1 Gb provided outputs that highly resembled WMS data at both genus and species levels and presented much higher accuracy taxonomic assignments and functional predictions than 16S, thereby representing a better and cost-efficient alternative to 16S for large-scale microbiome studies.
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http://dx.doi.org/10.3389/fmicb.2021.678319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361837PMC
July 2021

Self-referenced distribution of millimeter waves over 10  km optical fiber with high frequency stability.

Opt Lett 2021 Aug;46(16):3949-3952

In this Letter, an actively stabilized photonic system for millimeter-wave (mmW) signal distribution is proposed and experimentally demonstrated. By interlocking two baseband RF signals obtained from a dual-heterodyne detection through a single carrier compensation module, the phase fluctuations induced by the fiber transmission link is suppressed without the need of a local frequency reference. In the proof-of-concept experiment, a 108 GHz mmW is transmitted over a 10 km fiber link with a performance matching that of the back-to-back case. The feedback system reduces the phase noise of the delivered mmW signal by 37 dB and 28 dB at 0.1 Hz and 1 Hz frequency offset, respectively, and the long-term stability is improved by nearly two orders of magnitude.
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http://dx.doi.org/10.1364/OL.431889DOI Listing
August 2021

Identification of a Bromodomain-like Region in 15-Lipoxygenase-1 Explains Its Nuclear Localization.

Angew Chem Int Ed Engl 2021 Aug 13. Epub 2021 Aug 13.

Department Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy (GRIP), University of Groningen, Antonius Deusinglaan 1, 9713, AV, Groningen, The Netherlands.

Lipoxygenase (LOX) activity provides oxidative lipid metabolites, which are involved in inflammatory disorders and tumorigenesis. Activity-based probes to detect the activity of LOX enzymes in their cellular context provide opportunities to explore LOX biology and LOX inhibition. Here, we developed Labelox B as a potent covalent LOX inhibitor for one-step activity-based labeling of proteins with LOX activity. Labelox B was used to establish an ELISA-based assay for affinity capture and antibody-based detection of specific LOX isoenzymes. Moreover, Labelox B enabled efficient activity-based labeling of endogenous LOXs in living cells. LOX proved to localize in the nucleus, which was rationalized by identification of a functional bromodomain-like consensus motif in 15-LOX-1. This indicates that 15-LOX-1 is not only involved in oxidative lipid metabolism, but also in chromatin binding, which suggests a potential role in chromatin modifications.
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http://dx.doi.org/10.1002/anie.202106968DOI Listing
August 2021

Assessing vegetation stability to climate variability in Central Asia.

J Environ Manage 2021 Nov 6;298:113330. Epub 2021 Aug 6.

Department of Geography, Ghent University, Ghent, 9000, Belgium; Sino-Belgian Laboratory for Geo-Information, Ghent, 9000, Belgium.

The dramatic climate change has far-reaching impacts on vegetation in drylands such as Central Asia. Recent attempts to assess vegetation stability to short-term climate variability often account solely for vegetation sensitivity or resilience but ignore the composite effects of these two indicators. Meanwhile, our understanding of the vegetation stability at the seasonal scale remains insufficient. In this study, considering the cumulative effects of vegetation response to three key climate factors, we assessed the stability of vegetation in Central Asia using normalized difference vegetation index (NDVI) and the meteorological data from 1982 to 2014 by integrating vegetation sensitivity and resilience, and further identified the critical regions and seasons of vegetation that experience high risks of pending change. The results show that the sensitivity of vegetation has a strong correlation (R = 0.83, p < 0.001) with the aridity index (AI), with the vegetation of drier areas having lower sensitivities to climate variability. At the temporal scale, the sensitivity of vegetation to climate variability varied among different seasons. The average vegetation sensitivity index (VSI) is 41.17, 33.32 and 28.63 in spring, summer and autumn, respectively. Spatially, a trade-off between vegetation sensitivity and resilience is found both for the growing season (R = 0.67) and seasonal scale (R = 0.71, 0.32 and 0.43 for spring, summer and autumn, respectively), regions with high vegetation sensitivity were always accompanied by strong resilience. Based on the relationship between vegetation sensitivity and resilience, we further identify the critical regions and periods of vegetation with high change risk in Central Asia. Results suggest that herbaceous plants in semi-arid areas present high instability, especially in summer. This study offers a comprehensive perspective to assess vegetation stability to climate variability and the results will facilitate the protection of ecosystems and the implementation of sustainable development goals in Central Asia.
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http://dx.doi.org/10.1016/j.jenvman.2021.113330DOI Listing
November 2021

IL-36γ and IL-36Ra Reciprocally Regulate NSCLC Progression by Modulating GSH Homeostasis and Oxidative Stress-Induced Cell Death.

Adv Sci (Weinh) 2021 Aug 8:e2101501. Epub 2021 Aug 8.

Department of Gastrointestinal Surgery, College of Life Sciences, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.

The balance between antioxidants and reactive oxygen species (ROS) critically regulates tumor initiation and progression. However, whether and how the tumor-favoring redox status is controlled by cytokine networks remain poorly defined. Here, it is shown that IL-36γ and IL-36Ra reciprocally regulate the progression of non-small cell lung cancer (NSCLC) by modulating glutathione metabolism and ROS resolution. Knockout, inhibition, or neutralization of IL-36γ significantly inhibits NSCLC progression and prolongs survival of the Kras Tp53 and Kras Lkb1 mice after tumor induction, whereas knockout of IL-36Ra exacerbates tumorigenesis in these NSCLC mouse models and accelerates death of mice. Mechanistically, IL-36γ directly upregulates an array of genes involved in glutathione homeostasis to reduce ROS and prevent oxidative stress-induced cell death, which is mitigated by IL-36Ra or IL-36γ neutralizing antibody. Consistently, IL-36γ staining is positively and negatively correlated with glutathione biosynthesis and ROS in human NSCLC tumor biopsies, respectively. These findings highlight essential roles of cytokine networks in redox for tumorigenesis and provide potential therapeutic strategy for NSCLC.
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http://dx.doi.org/10.1002/advs.202101501DOI Listing
August 2021

Function of Polyamines in Regulating Cell Cycle Progression of Cultured Silkworm Cells.

Insects 2021 Jul 8;12(7). Epub 2021 Jul 8.

State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing 400715, China.

Background: Putrescine, spermidine, and spermine are polyamines that are ubiquitously distributed in prokaryotic and eukaryotic cells, which play important roles in cell proliferation and differentiation.

Methods: We investigated the expression profiles of polyamine pathway genes by qRT-PCR in different tissues of the lepidopteran silkworm. The polyamine levels in cultured silkworm cells were measured by HPLC. Spermidine and polyamine biosynthetic inhibitors were used for treating the cultured silkworm cells in order to clarify their effects on cell cycle progression.

Results: We identified the anabolic and catabolic enzymes that are involved in the polyamine biosynthetic pathway in silkworm. Transcriptional expression showed at least seven genes that were expressed in different silkworm tissues. Treatments of the cultured silkworm cells with spermidine or inhibitor mixtures of DFMO and MGBG induced or inhibited the expression of cell cycle-related genes, respectively, and thus led to changed progression of the cell cycle.

Conclusions: The present study is the first to identify the polyamine pathway genes and to demonstrate the roles of polyamines on cell cycle progression via regulation of the expression of cell cycle genes in silkworm.
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http://dx.doi.org/10.3390/insects12070624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306941PMC
July 2021

The role of SIRT1 in the basolateral amygdala in depression-like behaviors in mice.

Genes Brain Behav 2021 Aug 5:e12765. Epub 2021 Aug 5.

College of Forensic Science, Xi'an Jiaotong University, Shaanxi, China.

Previous investigations have implicated the basolateral amygdala (BLA) epigenetic mechanisms in the pathophysiology of depression. SIRT1 is a NAD+-dependent class III histone deacetylase, widely expresses in BLA. However, epigenetic mechanisms in the BLA under the regulation of SIRT1 in the depression are largely uncharacterized. Under the chronic unpredictable chronic mild stress (CUMS) mouse model, we used adeno-associated viral vectors (AAV) that encoded SIRT1-shRNA or SIRT1 to specifically knockdown or overexpress SIRT1 in BLA neurons, respectively. CUMS procedure induced significant depression symptoms including the decreased sucrose preference, the less bodyweight gained, the decreased immobile latency and the increased immobile time both in forced swim test (FST) and tail suspension test (TST). Knockdown of SIRT1 in BLA glutamatergic neurons reversed these depression-like behaviors and restored the synaptic abnormalities. Overexpression of SIRT1 in BLA glutamatergic neurons induced depression-like behaviors in non-stressed control mice. The result of protein expressions and ultrastructural changes were consistent with the behavioral results. Our study suggested that downregulation of SIRT1 in BLA has certain beneficial effect on CUMS-induced depression-like behaviors such as anorexia, anhedonia, hopelessness and despair. In addition, the increased expression of SIRT1 may be the immediate cause of depressive-like symptoms. The abnormal expression of SIRT1 may affect the transcriptional regulation mechanism and signaling protein acetylation, affecting neuroplasticity and ultimately contribute to MDD. In the stress-susceptible mice, these two mechanisms may co-exist, but the specific mechanism needs further research.
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http://dx.doi.org/10.1111/gbb.12765DOI Listing
August 2021

Co-occurrence of m.15992A>G and m.15077G>A is associated with a high penetrance of maternally inherited hypertension in a Chinese pedigree.

Am J Hypertens 2021 Aug 4. Epub 2021 Aug 4.

Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, and KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.

Background: Mitochondrial DNA (mtDNA) pathogenic variants have been identified to be associated with maternally inherited essential hypertension (MIEH). However, the distinctive clinical features and molecular pathogenesis of MIEH are not fully understood.

Methods: In this study, we collected a Chinese MIEH family with extraordinary higher penetrance of EH (88.89%) and early ages of onset (31-40 years old), and performed clinical and genetic characterization for this family. The complete mitochondrial genome of the proband was sequenced and analyzed.

Results: The maternally-related members in this family were presented with severe increased blood pressure (BP), left ventricular remodeling, and metabolic abnormalities. Through sequencing the entire mtDNA of the proband and performing systematic analysis of the mtDNA variants with a phylogenic approach, we identified a potentially pathogenic tRNA variant (m.15992A>G in the MT-TP gene) that may account for the MIEH in this family. One non-synonymous variant (m.15077G>A in the MT-CYB gene) was identified to play a synergistic role with m.15992A>G to cause a high penetrance of MIEH.

Conclusions: Our results, together with previous findings, have indicated that tRNA pathogenic variants in the mtDNA could act important roles in the pathogenesis of MIEH through reducing mitochondrial translation and disturbing mitochondrial function.
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http://dx.doi.org/10.1093/ajh/hpab123DOI Listing
August 2021

Ultrahigh Rate Capability and Lifespan MnCo2O4/Ni-MOF Electrode for High Performance Battery-Type Supercapacitor.

Chemistry 2021 Jul 31. Epub 2021 Jul 31.

Northwest Normal University, College of Chemistry and Chemical Engineering, Anning East Road 967, 730070, Lanzhou, CHINA.

MnCo2O4 is derived from a Co/Mn bimetallic MOF. Then Ni-MOF is directly grown on the surface of the obtained MnCo2O4 to form a nano-flower structure with small balls. Big surface area, abundant active sites of MnCo2O4 and porosity of Ni-MOF allow the prepared MnCo2O4/Ni-MOF composition material to deliver an excellent electrochemical performance. At the same time, appropriate thermal treatment temperature of the MnCo2O4 precursor is also very important for controlling the morphology of the obtained MnCo2O4 and electrochemical performances of the resulted composition material including electric conductivity, specific capacitance and rate performance. The prepared MnCo2O4-600/Ni-MOF shows an ultrahigh rate performance (when the current density increases from 1 to 10 A·g-1, the capacitance retention rate is as high as 93.41%) and good cycle stability (the assembled asymmetric supercapacitor advice delivers a capacitance retention rate of 94.74% after 20,000 charge and discharge cycles) as well as a relatively high specific capacitance. These excellent electrochemical properties indicate that MnCo2O4/Ni-MOF has a good application prospect in the market.
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http://dx.doi.org/10.1002/chem.202102008DOI Listing
July 2021

Young Woman With Hemoptysis.

Ann Emerg Med 2021 08;78(2):e13-e14

Department of Radiology, Yantai Yuhuangding Hospital, Qingdao University School of Medicine, Yantai, China. Electronic address:

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http://dx.doi.org/10.1016/j.annemergmed.2021.02.003DOI Listing
August 2021

Genome-Wide Analysis Identifies and as Novel Notch1 Transcriptional Targets in Thymocytes.

Front Cell Dev Biol 2021 12;9:703338. Epub 2021 Jul 12.

Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, China.

Recombination activating genes 1 () and are expressed in immature lymphocytes and essential for generating the vast repertoire of antigen receptors. Yet, the mechanisms governing the transcription of and remain to be fully determined, particularly in thymocytes. Combining cDNA microarray and ChIP-seq analysis, we identify and as novel Notch1 transcriptional targets in acute T-cell lymphoblastic leukemia (T-ALL) cells. We further demonstrate that Notch1 transcriptional complexes directly bind the and locus in not only T-ALL but also primary double negative (DN) T-cell progenitors. Specifically, dimeric Notch1 transcriptional complexes activate and through a novel -element bearing a sequence-paired site (SPS). In T-ALL and DN cells, dimerization-defective Notch1 causes compromised and expression; conversely, dimerization-competent Notch1 achieves optimal upregulation of both. Collectively, these results reveal Notch1 dimerization-mediated transcription as one of the mechanisms for activating and expression in both primary and transformed thymocytes. Our data suggest a new role of Notch1 dimerization in compelling efficient TCRβ rearrangements in DN progenitors during T-cell development.
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http://dx.doi.org/10.3389/fcell.2021.703338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311795PMC
July 2021

Revisiting the sex pheromone of the fall armyworm Spodoptera frugiperda, a new invasive pest in South China.

Insect Sci 2021 Jul 23. Epub 2021 Jul 23.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

The fall armyworm Spodoptera frugiperda is a worldwide serious agricultural pest, and recently invades in South China. Sex pheromone can be employed to monitor its population dynamics accurately in the field. However, the pheromone components previously reported by testing different geographic populations and strains are not consistent. On the basis of confirming that the S. frugiperda population from Yunnan province belonged to the corn strain, we analyzed the potential sex pheromone components in the pheromone gland extracts of females using the gas chromatography coupled with electroantennographic detection (GC-EAD), gas chromatography coupled with mass spectrometry (GC-MS) and electroantennography (EAG). The results show that (Z)-9-tetradecenal acetate (Z9-14:Ac), (Z)-11-hexadecenyl acetate (Z11-16:Ac), (Z)-7-dodecenyl acetate (Z7-12:Ac) or (E)-7-dodecenyl acetate (E7-12:Ac) with a ratio of 100: 15.8: 3.9 induced EAD responses to varying degree: Z9-14:Ac elicited a strong EAD response, Z7-12:Ac or E7-12:Ac elicited a small but clear EAD response, while Z11-16:Ac elicited a weak EAD response. The further single sensillum recording (SSR) showed that Z9-14:Ac and Z7-12:Ac induced dose-dependent activities in two types (A and B) of sensilla in male antennae, respectively, while the sensilla in response to E7-12:Ac and Z11-16:Ac was not recorded. Finally, wind tunnel tests reveal that Z9-14:Ac and Z7-12:Ac are two principal sex pheromone components of the tested population. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1744-7917.12956DOI Listing
July 2021

Redox of naphthalenediimide radicals in a 3D polyimide for stable Li-ion batteries.

Chem Commun (Camb) 2021 Aug;57(63):7810-7813

Department of Materials Science and Engineering, Guangdong-Hong Kong-Macao Joint Laboratory for Photonic-Thermal-Electrical Energy Materials and Devices, Southern University of Science and Technology, Shenzhen 518055, Guangdong, P. R. China.

A 3D polyimide is designed as an organic cathode for Li-ion batteries. Detailed characterization and DFT simulations demonstrate that the 3D polyimide undergoes the redox of naphthalenediimide radicals and the rigidity effect of the 3D structure contributes to the stability of the radical intermediates for high-performance organic batteries.
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http://dx.doi.org/10.1039/d1cc02426dDOI Listing
August 2021

Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.

PLoS Genet 2021 Jul 15;17(7):e1009527. Epub 2021 Jul 15.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Glucosinolates are token stimuli in host selection of many crucifer specialist insects, but the underlying molecular basis for host selection in these insects remains enigmatic. Using a combination of behavioral, electrophysiological, and molecular methods, we investigate glucosinolate receptors in the cabbage butterfly Pieris rapae. Sinigrin, as a potent feeding stimulant, elicited activity in larval maxillary lateral sensilla styloconica, as well as in adult medial tarsal sensilla. Two P. rapae gustatory receptor genes PrapGr28 and PrapGr15 were identified with high expression in female tarsi, and the subsequent functional analyses showed that Xenopus oocytes only expressing PrapGr28 had specific responses to sinigrin; when ectopically expressed in Drosophila sugar sensing neurons, PrapGr28 conferred sinigrin sensitivity to these neurons. RNA interference experiments further showed that knockdown of PrapGr28 reduced the sensitivity of adult medial tarsal sensilla to sinigrin. Taken together, we conclude that PrapGr28 is a gustatory receptor tuned to sinigrin in P. rapae, which paves the way for revealing the molecular basis of the relationships between crucifer plants and their specialist insects.
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http://dx.doi.org/10.1371/journal.pgen.1009527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282186PMC
July 2021

Erteng-Sanjie Capsule Enhances Chemosensitivity of 5-Fluorouracil in Tumor-Bearing Nude Mice with Gastric Cancer by Inhibiting Notch1/Hes1 Signaling Pathway.

Evid Based Complement Alternat Med 2021 23;2021:9980565. Epub 2021 Jun 23.

Department of Oncology, Shanxi Province Hospital of Traditional Chinese Medicine, Shanxi Province Academy of Traditional Chinese Medicine, Shanxi, Taiyuan 030012, China.

Gastric cancer is one of the most common cancers worldwide. This study investigated the chemosensitivity-enhancing effects of Erteng-Sanjie capsule (ETSJC) in combination with 5-fluorouracil (5-FU) on gastric cancer and its possible underlying mechanisms. The study established a subcutaneous xenograft model of human gastric cancer. The animals were divided into five groups: the control group, the 5-FU group, the 5-FU + ETSJC low-dose group, the 5-FU + ETSJC medium-dose group, and the 5-FU + ETSJC high-dose group. The tumor volume and tumor weight were calculated. TUNEL staining was used to evaluate cell apoptosis. Immunohistochemical analysis was used to detect the expression of Ki67 cells and the CD31 microvessel density in tumors. Simultaneously, western blot analysis was applied to detect the expression of caspase-3, Bax, Bcl-2, Notch1, and Hes1 proteins. Compared with the control group, tumor volume and weight in the 5-FU and 5-FU + ETSJC groups were inhibited. Moreover, compared with the 5-FU group, tumor volume and weight were significantly inhibited in the 5-FU + ETSJC groups. The numbers of Ki67 cells, CD31 microvessel density, and the expression of Bcl-2, Notch1, and Hes1 proteins were markedly decreased in the combination group when compared with the chemotherapy alone group. The numbers of TUNEL cells and the expression of Bax and caspase-3 proteins were significantly increased in the 5-FU + ETSJC groups when compared with the 5-FU group. The therapeutic effects were demonstrated to be dose dependent. In conclusion, the findings of the study showed that ETSJC improved the chemosensitivity of 5-FU by blocking Notch1/Hes1 signaling pathway in gastric cancer-bearing mice.
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http://dx.doi.org/10.1155/2021/9980565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249139PMC
June 2021

High-throughput screening of circRNAs reveals novel mechanisms of tuberous sclerosis complex-related renal angiomyolipoma.

Hum Genomics 2021 Jul 9;15(1):43. Epub 2021 Jul 9.

Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: Tuberous sclerosis complex (TSC) is a rare autosomal dominant disease characterized by lesions throughout the body. Our previous study showed the abnormal up-regulation of miRNAs plays an important part in the pathogenesis of TSC-related renal angiomyolipoma (TSC-RAML). circRNAs were known as important regulators of miRNA, but little is known about the circRNAs in TSC-RAMLs.

Methods: Microarray chips and RNA sequencing were used to identify the circRNAs and mRNAs that were differently expressed between the TSC-RAML and normal kidney tissue. A competitive endogenous RNA (ceRNA) regulatory network was constructed to reveal the regulation of miRNAs and mRNAs by the circRNAs. The biological functions of circRNA and mRNA were analyzed by pathway analysis. Microenvironmental cell types were estimated with the MCP-counter package.

Results: We identified 491 differentially expressed circRNAs (DECs) and 212 differentially expressed genes (DEGs), and 6 DECs were further confirmed by q-PCR. A ceRNA regulatory network which included 6 DECs, 5 miRNAs, and 63 mRNAs was established. Lipid biosynthetic process was significantly up-regulated in TSC-RAML, and the humoral immune response and the leukocyte chemotaxis pathway were found to be down-regulated. Fibroblasts are enriched in TSC-RAML, and the up-regulation of circRNA_000799 and circRNA_025332 may be significantly correlated to the infiltration of the fibroblasts.

Conclusion: circRNAs may regulate the lipid metabolism of TSC-RAML by regulation of the miRNAs. Fibroblasts are enriched in TSC-RAMLs, and the population of fibroblast may be related to the alteration of circRNAs of TSC-RAML. Lipid metabolism in fibroblasts is a potential treatment target for TSC-RAML.
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http://dx.doi.org/10.1186/s40246-021-00344-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272316PMC
July 2021
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