Publications by authors named "Hao Feng"

845 Publications

Identification of antibacterial metabolites produced by a marine bacterium Halobacillus marinus HMALI004.

J Appl Microbiol 2022 Aug 5. Epub 2022 Aug 5.

Biology Department and Institute of Marine Sciences, College of Science, and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China.

Aims: This study examined and characterized the extract for metabolites of Halobacillus marinus HMALI004 to understand their antibacterial activities against opportunistic marine pathogens, i.e., Vibrio parahaemolyticus and Vibrio cholerae.

Methods And Results: The bacterial strain HMALI004 was characterized as Halobacillus marinus, and an antibacterial spectral test revealed its inhibition against two opportunistic marine pathogens (V. parahaemolyticus and V. cholera). Fermentation broth of strain HMALI004 was subjected to column chromatography and high-performance liquid chromatography (HPLC) to separate antibacterial substances. Two compounds were successfully isolated and identified as 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid by mass spectrometry (MS) and nuclear magnetic resonance (NMR). The minimal inhibition concentration (MIC) values of 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid for V. parahaemolyticus were 25 μg/mL, while their MIC values for V. cholerae were 50 and 100 μg/mL, respectively. The reactive oxygen species (ROS) production of two pathogen strains treated with 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid were detected to investigate the antimicrobial mechanism. The results suggested that 4-chloro-1H-pyrrole-2-carboxylic acid exerted enhanced ROS production in V. parahaemolyticus, whereas 1H-pyrrole-2-carboxylic acid had a weaker effect. Both compounds caused a significant rise in ROS production in V. cholerae, causing severe damage to the cell wall and cytoplasm, leading to cell death.

Conclusions: The bacterium H. marinus HMALI004 was isolated from a shrimp pond and was found to produce antimicrobial compounds which could inhibit the growth of opportunistic marine pathogens V. parahaemolyticus and V. cholerae by increasing ROS.

Significance And Impact Of The Study: Successfully isolated antibacterial-producing strain, H. marinus HMALI004, and its antimicrobial compounds could be used as biological control agents for marine pathogens.
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http://dx.doi.org/10.1111/jam.15764DOI Listing
August 2022

The clinical significance of integrin subunit alpha V in cancers: from small cell lung carcinoma to pan-cancer.

BMC Pulm Med 2022 Aug 4;22(1):300. Epub 2022 Aug 4.

Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People's Republic of China.

Background: Little is known about the relationship between integrin subunit alpha V (ITGAV) and cancers, including small cell lung cancer (SCLC).

Methods: Using large sample size from multiple sources, the clinical roles of ITGAV expression in SCLC were explored using differential expression analysis, receiver operating characteristic curves, Kaplan-Meier curves, etc. RESULTS: Decreased mRNA (SMD = - 1.05) and increased protein levels of ITGAV were detected in SCLC (n = 865). Transcription factors-ZEB2, IK2F1, and EGR2-may regulate ITGAV expression in SCLC, as they had ChIP-Seq (chromatin immunoprecipitation followed by sequencing) peaks upstream of the transcription start site of ITGAV. ITGAV expression made it feasible to distinguish SCLC from non-SCLC (AUC = 0.88, sensitivity = 0.78, specificity = 0.84), and represented a risk role in the prognosis of SCLC (p < 0.05). ITGAV may play a role in cancers by influencing several immunity-related signaling pathways and immune cells. Further, the extensive pan-cancer analysis verified the differential expression of ITGAV and its clinical significance in multiple cancers.

Conclusion: ITGAV served as a potential marker for prognosis and identification of cancers including SCLC.
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http://dx.doi.org/10.1186/s12890-022-02095-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354352PMC
August 2022

Characteristics of Physicians Obtaining Micrographic Dermatologic Surgery Board Certification in 2021.

Dermatol Surg 2022 Aug;48(8):896-898

Department of Dermatology, University of Connecticut, Farmington, Connecticut.

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http://dx.doi.org/10.1097/DSS.0000000000003519DOI Listing
August 2022

PAK6 promotes homologous-recombination to enhance chemoresistance to oxaliplatin through ATR/CHK1 signaling in gastric cancer.

Cell Death Dis 2022 Jul 28;13(7):658. Epub 2022 Jul 28.

Department of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, 510515, China.

Chemoresistance remains the primary challenge of clinical treatment of gastric cancer (GC), making the biomarkers of chemoresistance crucial for treatment decision. Our previous study has reported that p21-actived kinase 6 (PAK6) is a prognostic factor for selecting which patients with GC are resistant to 5-fluorouracil/oxaliplatin chemotherapy. However, the mechanistic role of PAK6 in chemosensitivity remains unknown. The present study identified PAK6 as an important modulator of the DNA damage response (DDR) and chemosensitivity in GC. Analysis of specimens from patients revealed significant associations between the expression of PAK6 and poorer stages, deeper invasion, more lymph node metastases, higher recurrence rates, and resistance to oxaliplatin. Cells exhibited chemosensitivity to oxaliplatin after knockdown of PAK6, but showed more resistant to oxaliplatin when overexpressing PAK6. Functionally, PAK6 mediates cancer chemoresistance by enhancing homologous recombination (HR) to facilitate the DNA double-strand break repair. Mechanistically, PAK6 moves into nucleus to promote the activation of ATR, thereby further activating downstream repair protein CHK1 and recruiting RAD51 from cytoplasm to the DNA damaged site to repair the broken DNA in GC. Activation of ATR is the necessary step for PAK6 mediated HR repair to protect GC cells from oxaliplatin-induced apoptosis, and ATR inhibitor (AZD6738) could block the PAK6-mediated HR repair, thereby reversing the resistance to oxaliplatin and even promoting the sensitivity to oxaliplatin regardless of high expression of PAK6. In conclusion, these findings indicate a novel regulatory mechanism of PAK6 in modulating the DDR and chemoresistance in GC and provide a reversal suggestion in clinical decision.
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http://dx.doi.org/10.1038/s41419-022-05118-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334622PMC
July 2022

Disseminierte braune Papeln an Rumpf und Extremitäten eines älteren Mannes.

J Dtsch Dermatol Ges 2022 07;20(7):1037-1040

Department of Dermatology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases, Beijing, China.

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http://dx.doi.org/10.1111/ddg.14793_gerDOI Listing
July 2022

A 4-Arm Small Molecule Acceptor with High Photovoltaic Performance.

Angew Chem Int Ed Engl 2022 Jul 26. Epub 2022 Jul 26.

Yunnan University, School of Materials and Energy, CHINA.

Manipulating the backbone of small molecule acceptors (SMAs) is of particular importance in developing efficient organic solar cells (OSCs). The common design is constructing 2 -arm SMAs with linear or curved backbones. Herein, we report an acceptor 4A-DFIC with a 4-arm backbone unexpectedly generated in the reaction of an electron-rich aromatic diamine and hexaketocyclohexane. Single -crystal X-ray diffraction analysis indicates the rigid and twisted molecular plane and the effective molecular stacking of 4A-DFIC in solid state. 4A-DFIC shows a low bandgap of 1.40 eV and excellent light-harvesting capability from visible to near-infrared region. Binary and ternary OSCs based on 4A-DFIC gave power conversion efficiencies (PCEs) of 15.76% and 18.60% (certified 18.1%), respectively, which are the highest PCEs for multi-arm SMA-based OSCs to date.
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http://dx.doi.org/10.1002/anie.202207762DOI Listing
July 2022

A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic .

Front Plant Sci 2022 7;13:927253. Epub 2022 Jul 7.

Agro-Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Halophytic is a perennial grass which has evolved many distinctive salt-adaptive mechanisms. Our previous studies indicated it could thrive under salt stress through maintaining better K and Na homeostasis. Stress-responsive HbCIPK2 can phosphorylate K channel HbVGKC1 and Na transporter HbSOS1L to prevent Na accumulation and K reduction, hence pathway was not detected in glycophytic plants. In this study, we cloned the inducible promoter of by genome-walking, and identified a novel transcriptional regulator HbERF6 through yeast one-hybrid screening. HbERF6 functioned as a transcription factor which can bind to the GCC-box of the promoter to activate its expression. transgenic lines in improved salt tolerance compared with wild type, and especially induced () expression, resulting in K/Na homeostasis to enhance salt tolerance. All the results confirmed the inducible function of HbERF6 for CIPK genes during salt tolerance. This regulatory network that integrates transcriptional regulation and post-translation modification will unravel a novel salt stress-responsive mechanism, highlighting the value and utilization of the halophytic resource.
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http://dx.doi.org/10.3389/fpls.2022.927253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302439PMC
July 2022

Intelligent Correction Method of Shooting Action Based on Computer Vision.

Comput Intell Neurosci 2022 11;2022:8753473. Epub 2022 Jul 11.

Police Physical Education Department, Hebei Vocational College for Correctional Police, Shijiazhuang 050081, China.

Aiming at the problem that the students' long-term use of nonstandard shooting action leads to poor basketball teaching effect, an intelligent correction method of shooting action based on computer vision is proposed. Combined with the principle of computer vision, the image acquisition model of basketball shooting action is constructed. The edge contour and adaptive feature segmentation of basketball images are detected, and abnormal shooting movements are recognized. The intelligent correction model of shooting action is constructed, and the intelligent correction of shooting action is realized. Finally, through experiments, it is proved that the visual analysis and intelligent correction effect of basketball shooting action are obviously better, and it can correct shooting action in real time and accurately.
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http://dx.doi.org/10.1155/2022/8753473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293490PMC
July 2022

Effect of Perioperative Interleukin-6 and Tumor Necrosis Factor- on Long-Term Outcomes in Locally Advanced Gastric Cancer: Results from the CLASS-01 Trial.

J Immunol Res 2022 8;2022:7863480. Epub 2022 Jul 8.

Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Little is known about the relation between perioperative inflammatory changes and long-term survival in cancer patients. The aim of the study was to assess the association of perioperative serum interleukin-6 (IL6) and tumor necrosis factor- (TNF) levels with the 5-year overall survival in locally advanced gastric cancer.

Methods: The 135 eligible patients in one center of Nanfang Hospital were retrieved from CLASS-01 trial (NCT01609309), an open-label, multicenter, randomized clinical noninferiority trial conducted at 14 centers in China. Serum IL6 and TNF levels were tested before surgery, and on postoperative day (POD) 1, POD3, and POD5, respectively, referring to IL6_0, IL6_1, IL6_3, and IL6_5 and TNF_0, TNF_1, TNF_3, and TNF_5. Kaplan-Meier methods and COX models were used for survival analysis.

Results: High levels of IL6_0 (≥3.67 pg/mL) and TNF_0 (≥14.8 pg/mL) presented worse disease-free survival (DFS) ( = 0.0057 for IL6_0 and = 0.0014 for TNF_0) and overall survival (OS) ( = 0.0021 for IL6_0 and = 0.0019 for TNF_0). Both high IL6_0 and high IL6_5 levels indicated worse prognosis than other combinations ( = 0.0045 for DFS and = 0.0022 for OS). In multivariate analysis, both high IL6_0 and high IL6_5 levels were significantly associated with poor DFS (HR = 4.29, 95% CI: 1.42-12.95, = 0.01) and OS (HR = 4.11, 95% CI: 1.35-12.49, = 0.013) after adjustment of tumor stage and TNF_0. Also, high IL6_5 level was identified as the independent-related factor for postoperative infectious complications (OR = 2.69, 95% CI: 1.03-7.01, = 0.043).

Conclusions: Perioperative high serum IL6 and TNF levels are negatively associated with 5-year survival outcomes in patients with locally advanced gastric cancer, indicating the potential survival benefits from perioperative anti-inflammatory treatment.
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http://dx.doi.org/10.1155/2022/7863480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289757PMC
July 2022

Cancer-associated fibroblasts strengthen cell proliferation and EGFR TKIs resistance through aryl hydrocarbon receptor dependent signals in non-small cell lung cancer.

BMC Cancer 2022 Jul 13;22(1):764. Epub 2022 Jul 13.

The First People's Hospital of Shuangliu District, Chengdu (West China Airport Hospital of Sichuan University), Near 51 Kangle Lane, Chengdu City, 610200, Sichuan Province, China.

The tumor microenvironment is a dynamic cellular milieu that interacts with cancer cells and promotes tumor progression and metastasis. However, the specific mechanisms by which the tumor microenvironment impacts cancer cells' behaviors remain poorly understood. In this study, enriched cancer-associated fibroblasts (CAFs) were observed in tumor tissues isolated from epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) resistant non-small cell lung cancer (NSCLC) patients. CAFs isolated from tumor tissues were capable of producing tryptophan metabolite kynurenine (Kyn), which significantly increased the proliferation and EGFR TKIs resistance of NSCLC cells. In this study, it was further observed that the activation of tryptophan 2,3-dioxygenase (TDO) in CAFs, resulted in the enhanced capability of tryptophan metabolism in them compared to normal fibroblasts. As a result, Kyn produced by CAFs facilitated the up-regulation of Aryl Hydrocarbon Receptor (AhR) signals in NSCLC, thereby resulting in the downstream ATK and ERK signaling pathways activation. Finally, inhibition of AhR signals efficiently prevented tumor growth and development of EGFR TKIs resistance, eventually improved the outcome of EGFR TKIs, and described a promising therapeutic strategy for NSCLC.
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http://dx.doi.org/10.1186/s12885-022-09877-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281029PMC
July 2022

Dermatology Workforce in the United States: Part II - Patient Outcomes, Challenges, and Potential Solutions.

J Am Acad Dermatol 2022 Jul 1. Epub 2022 Jul 1.

Department of Dermatology, University of Connecticut Health Center, Farmington, CT.

This continuing medical education (CME) series assesses key features and ongoing transformations within the dermatology workforce. Part 1 of this series described precipitants and implications of recent workforce changes. Part 2 reviews the influence that these workforce dynamics have had on patient access, outcomes, and satisfaction. Additionally, it assesses potential solutions for optimizing care for underserved groups and the broader dermatologic patient population.
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http://dx.doi.org/10.1016/j.jaad.2022.06.1192DOI Listing
July 2022

Dermatology Workforce in the United States - Part 1: Overview, Transformations, and Implications.

J Am Acad Dermatol 2022 Jul 1. Epub 2022 Jul 1.

Department of Dermatology, University of Connecticut Health Center, Farmington, CT.

The dermatology workforce continues to evolve to meet the growing and diversified demands of the United States population. Part 1 of this continuing medical education (CME) series is designed to provide an overview of the dermatology workforce as well as delineate the motivators and socio-economic implications of significant workforce transformations which are impacting dermatologic health care. Part 2 of the series will consider the impact of workforce challenges on patient outcomes and discuss potential actions that may help to optimize workforce organization and care delivery.
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http://dx.doi.org/10.1016/j.jaad.2022.06.1191DOI Listing
July 2022

Variation in Ratios of Submitted Charges to Allowed Medicare Payment for Dermatologic Services.

JAMA Dermatol 2022 Jun 29. Epub 2022 Jun 29.

Department of Dermatology, University of Connecticut Health Center, Farmington.

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http://dx.doi.org/10.1001/jamadermatol.2022.2214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244768PMC
June 2022

Effect of early-life exposure to PM on childhood asthma/wheezing: a birth cohort study.

Pediatr Allergy Immunol 2022 06;33(6)

Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou, China.

Background: Although studies have investigated the association between early-life exposure to fine particulate matter (PM ) and childhood asthma/wheezing, results are inconsistent and the susceptible exposure window remains largely unknown.

Methods: A prospective birth cohort study was conducted to recruit pregnant women during their early pregnancy, and to follow up them and their children up to 3-4 years old. Diagnosis of asthma/wheezing was extracted from children's medical records. A spatiotemporal land-use regression (ST-LUR) model was used to assess maternal exposure to PM during pregnancy and their children's exposure after birth. The Cox proportional hazards model and accelerated failure time model (for violation of proportional hazards assumption) were applied to estimate the effects of prenatal and postnatal exposures to PM on the risk of childhood asthma/wheezing.

Results: A total of 3725 children were included, and 392 children (10.52%) were diagnosed with asthma/wheezing. Both prenatal and postnatal exposures to PM were positively associated with the risk of asthma/wheezing. Each interquartile range (IQR) increment in PM exposure during the entire pregnancy (4.8 μg/m ) and the period from birth to the end of follow-up (1.5 μg/m ) was associated with adjusted hazard ratios (HRs) of 1.44 [95% confidence interval (CI): 1.13, 1.85] and 2.74 (95% CI: 2.59, 2.91), respectively. Subgroup analyses showed greater HRs for PM exposures during the pseudoglandular stage (6-16 gestational weeks [GWs]: IQR = 4.8 μg/m , HR = 1.10, 95% CI: 1.02, 1.18) and canalicular stage (16-24 GWs: IQR = 4.8 μg/m , HR = 1.13, 95% CI:1.03, 1.23) than other stages, and also showed significant effects in the first three-year period after birth (IQR = 1.5 μg/m , HR = 2.37, 95% CI: =2.24, 2.51).

Conclusion: Higher prenatal and postnatal PM exposures may increase the risk of childhood asthma/wheezing. The pseudoglandular stage, canalicular stage, and the first three years after birth may be key susceptible to exposure windows.
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http://dx.doi.org/10.1111/pai.13822DOI Listing
June 2022

Removal of Cr(VI) from aqueous solution by a novel ZnO-sludge biochar composite.

Environ Sci Pollut Res Int 2022 Jun 27. Epub 2022 Jun 27.

Xi'an No.5 Reclaimed Water Plant, Xi'an, 710000, China.

The incorporation of ZnO into biochar has become a promising way to obtain adsorbents with enhanced adsorption capacity. In this study, a low-cost ZnO-sludge biochar composite (ZBC) was prepared by a simply in situ method using sewage sludge biochar (SBC) and zinc acetate, as well as employed for Cr(VI) adsorption in water. The results of XPS and FT-IR suggested that the ZBC surface had more functional groups such as -COOH, -OH, -C-O, ZnO, etc. Compared with SBC, the BET-specific surface area of the ZBC increased from 8.82 to 41.24 m·g, which provides potential advantages for Cr(VI) uptake. Benefiting from ZnO incorporation, about an 18% increase in Cr(VI) removal efficiency was obtained. The maximum removal efficiency and equilibrium adsorption amount of ZBC for Cr(VI) reached 98.4% and 33.87 mg·g, respectively. The adsorption was spontaneous and endothermic nature, and coincided nicely with pseudo-second-order kinetics and Langmuir isotherm. The analyses indicated that Cr(VI) removal by ZBC was predominantly via electrostatic attraction, surface complexation, ion exchange, and reduction. This study provided valuable insights into the problem of sludge disposal and provided a new and effective method for Cr(VI) removal.
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http://dx.doi.org/10.1007/s11356-022-21616-wDOI Listing
June 2022

Aggregation-Induced Emission (AIE) and Magnetic Resonance Imaging Characteristics for Targeted and Image-Guided siRNA Therapy of Hepatocellular Carcinoma.

Adv Healthc Mater 2022 Jun 24:e2200579. Epub 2022 Jun 24.

Shanghai Institute of Transplantation, Shanghai, 200127, China.

Hepatocellular carcinoma (HCC) is the most common form of primary liver cancer and remains a global health challenge. Small interfering RNA (siRNA) is a promising therapeutic modality that blocks multiple disease-causing genes without impairing cell structures. However, siRNA therapeutics still have off-target proportion and lack effective quantitative analysis method in vivo. Thus, a novel theragnostic nanoparticle with dual-mode imaging is synthesized for targeted and image-guided siRNA therapy of HCC. Survivin siRNA is carried by Poly-ethylenimine (PEI) and interacted with T7-AIE/Gd NPs, which are self-assembled of DSPE-PEG-DTPA(Gd), DSPE-PEG-Mal, DSPE-PEG-PEI, and TPE. The resulting theragnostic nanoparticles exhibit lower toxicity and high therapeutic effect, and excellent T1-weighted magnetic resonance imaging (MRI) and aggregation-induced emission (AIE) imaging performance. Moreover, in vivo MRI and AIE imaging indicate that this kind of theragnostic nanoparticles rapidly accumulates in the tumor due to active targeting and enhanced permeability and retention (EPR) effects. [email protected] suppresses HCC tumor growth by inducing autophagy and destabilizes DNA integrity in tumor cells. The results suggest that T7-AIE-Gd nanoparticles carrying Survivin siRNA with dual-mode imaging characteristics are promising for targeted and image-guided siRNA therapy of hepatocellular carcinoma.
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http://dx.doi.org/10.1002/adhm.202200579DOI Listing
June 2022

Disseminated brown papules on the trunk and extremities of an elderly man.

J Dtsch Dermatol Ges 2022 07 24;20(7):1037-1040. Epub 2022 Jun 24.

Department of Dermatology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases, Beijing, China.

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http://dx.doi.org/10.1111/ddg.14793DOI Listing
July 2022

Transplantation for EASL-CLIF and APASL acute-on-chronic liver failure (ACLF) patients: The to evaluate long-term post-Transplant outcomes.

EClinicalMedicine 2022 Jul 4;49:101476. Epub 2022 Jun 4.

Department of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Background: The forecast accuracy of the European Association for the Study of the Liver-Chronic Liver Failure (EASL-CLIF) and Asian Pacific Association for the Study of the Liver (APASL) acute-on-chronic liver failure (ACLF) criteria in assessing long-term outcomes after liver transplantation (LT) is still unclear, especially when the staging of the two standards is inconsistent.

Methods: A retrospective cohort () including 565 patients from January 2015 to June 2021 was conducted. The 28 and 90 days, 1- and 3-years overall survival (OS) after LT were compared between different grades.

Findings: Total of 162 (28.7%) and 230 (40.7%) patients met the ACLF standards. In the EASL-CLIF criteria, the 3-year OS rates were 83·0%, 80·3%, and 69·8% for ACLF1-3, respectively. In the APASL criteria, the 3-year OS rates were 85·7% for APASL ACLF Research Consortium (AARC)-1, similar to ACLF-1. The 3-year OS rates were 84·5% for AARC-2, which were slightly better than ACLF-2. Regarding AARC-3, the 3-year OS rate was 5·8% higher than ACLF-3. For patients who met neither set of criteria for ACLF, the 3-year OS rates were 89·8%. The multivariate analysis showed that alanine aminotransferase >100 U/L, respiration failure, and cerebral failure were independent risk factors for post-LT death.

Interpretation: This study provides the first large-scale long-term follow-up data in Asia. Both criteria showed favorable distinguishing ability for post-LT survival. Patients with ACLF had a higher post-LT mortality risk, and ACLF-3 and AARC-3 correlated with significantly greater mortality.

Funding: National Natural Science Foundation of China and Science and Technology Commission of Shanghai Municipality.
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http://dx.doi.org/10.1016/j.eclinm.2022.101476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167862PMC
July 2022

AdipoR1 Regulates Ionizing Radiation-Induced Ferroptosis in HCC cells through Nrf2/xCT Pathway.

Oxid Med Cell Longev 2022 13;2022:8091464. Epub 2022 Jun 13.

School of Public Health and Management, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.

Radiotherapy has been used for decades in the treatment of liver cancer. We previously found that adiponectin receptor (AdipoR1) is a prognostic biomarker for hepatoma carcinoma (HCC) after stereotactic body radiation therapy (SBRT) and blocking AdipoR1 enhances radiation sensitivity in hepatoma carcinoma cells. In the current study, we aimed to elucidate the roles of AdipoR1 in ionizing radiation- (IR-) induced radiosensitivity by activating ferroptosis pathway in HCC cells. We found that IR upregulated the expression of AdipoR1 and furthermore promoted the protein stability of transcription factor Nrf2, Nrf2 binded to the xCT promoter and increased xCT transcription and expression, and this directly contributed to the protective function in the early stage of radiation in HCC cells. AdipoR1 knockdown significantly inhibited expression of Nrf2 and xCT and, furthermore, increased both IR- and erastin-induced ferroptosis, which could be abolished by the rescue of Nrf2 and xCT. For the first time, we found that radiation-induced ferroptosis was mediated by AdipoR1-Nrf2-xCT pathway in HCC cells. These results provide new insights to the development and application of novel therapeutic strategies for hepatoma carcinoma.
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http://dx.doi.org/10.1155/2022/8091464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208988PMC
June 2022

α7 Nicotinic Acetylcholine Receptor May Be a Pharmacological Target for Perioperative Neurocognitive Disorders.

Front Pharmacol 2022 3;13:907713. Epub 2022 Jun 3.

Department of Anesthesiology, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, China.

The α7 nicotinic acetylcholine receptor (α7nAChR) is a promising therapeutic target in neurodegenerative diseases. This study examined the effects of surgery and anesthesia on α7nAChR expression in the central nervous system and determined the mechanisms by which α7nAChR mediates neuroprotection in perioperative neurocognitive disorders (PNDs) in aged mice. Eighteen-month-old male C57BL/6J mice underwent aseptic laparotomy under isoflurane anesthesia, maintaining spontaneous ventilation to establish the PNDs model. Agonists and antagonists of the α7nAChR and tropomyosin receptor kinase B (TrkB) receptors were administered before anesthesia. The α7nAChR expression, peripheral as well as hippocampal interleukin-1β (IL-1β), and the brain-derived neurotrophic factor (BDNF) levels were assessed. Separate cohorts of aged mice were tested for cognitive decline using the Morris water maze (MWM). Surgery and anesthesia significantly suppressed α7nAChR expression in the hippocampus and cortex. Surgery-induced IL-1β upregulation in the serum as well as hippocampus and hippocampal microglial activation were reversed by the α7nAChR agonist A significant reduction in the hippocampal BDNF levels were also observed. The α7nAChR stimulation reversed, and α7nAChR suppression promoted BDNF reduction in the hippocampus. Blocking the BDNF/TrkB signaling pathway abolished α7nAChR-induced neuroprotection in PNDs, as evidenced by poor cognitive performance in the MWM test. These data reveal that α7nAChR plays a key role in PNDs. The mechanisms of the anti-inflammatory pathway and BDNF/TrkB signaling pathways are involved in α7nAChR-meidiated neuroprotection in PNDs.
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http://dx.doi.org/10.3389/fphar.2022.907713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204176PMC
June 2022

[Mechanism of Astragali Radix-Puerariae Lobatae Radix combination in regulating type 2 diabetes mellitus through AMPK signaling pathway: based on network pharmacology and experimental verification].

Zhongguo Zhong Yao Za Zhi 2022 May;47(10):2738-2749

Jiangxi University of Chinese Medicine Nanchang 330004, China.

This study aims to explore the mechanism of Astragali Radix-Puerariae Lobatae Radix(AP) combination in the treatment of type 2 diabetes mellitus(T2 DM) based on network pharmacology and experiment. The effective components and targets of the pair were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and targets of T2 DM from each disease database. On this basis, the common targets of the medicinals and the disease were screened out. The protein-protein interaction(PPI) network was established based on STRING. Then Cytoscape 3.7.1 was employed for visualization of the common targets and the network topology analysis of key targets, followed by Gene Ontology(GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment of core targets by DAVID. Thereby, the possible molecular mechanism was unveiled. High-fat diet was combined with streptozotocin(STZ, injected into tail vein) for T2 DM rat modeling. Rats were classified into the normal group, model group, positive control group(metformin hydrochloride), AP high-dose, medium-dose, and low-dose groups. After 4 weeks of intragastric administration, serum fasting blood glucose(FBG), fasting insulin(FINS), aspartate aminotransferase(AST), alanine aminotransferase(ALT), triglyceride(TG), total cholesterol(TC), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C), interleukin(IL)-6, and tumor necrosis factor(TNF)-α of rats in each group were measured. The expression of insulin receptor substrate-2(IRS-2), adenosine monophosphate-activated protein kinase(AMPK), phosphorylated AMPK(p-AMPK), glucose 6 phosphatase(G6 Pase), and phosphoenolpyruvate carboxy kinase(Pepck) in rat liver was detected by Western blot. A total of 131 core targets of the combination in the treatment of T2 DM were screened out, among which protein kinase B(AKT) 1, mitogen-activated protein kinase(MAPK) 1, TNF-α, IL-6 were more critical. KEGG enrichment analysis suggested that the combination decreased blood glucose mainly through PI3 K/AKT signaling pathway, AMPK signaling pathway, TNF signaling pathway, and MAPK signaling pathway. The levels of FBG and FINS were lower and the glycogen level was higher in the AP high-dose and medium-dose groups than in the model group. The levels of AST, ALT, TG, and LDL-C in the three AP groups and the level of TC in AP high-dose and low-dose groups decreased compared with those in the model group. Levels of IL-6 and TNF-α were lower in AP high-dose and medium-dose groups than in the model group. The expression of IRS-2, AMPK, and p-AMPK was higher and that of G6 Pase and Pepck was lower in AP high-dose group than in the model group. Thus, the combination had multi-component, multi-target, and multi-pathway characteristics in the treatment of T2 DM. It may regulate AMPK signaling pathway through IL-6 and TNF-α to influence insulin resistance, glycogen synthesis, gluconeogenesis, islet β cell transport, and inflammatory response, thereby exerting therapeutic effect on T2 DM.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211216.703DOI Listing
May 2022

Chronic Cervicitis and Cervical Cancer Detection Based on Deep Learning of Colposcopy Images Toward Translational Pharmacology.

Front Pharmacol 2022 27;13:911962. Epub 2022 May 27.

Department of Dermatology, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal University), Changsha, China.

With the rapid development of deep learning, automatic image recognition is widely used in medical development. In this study, a deep learning convolutional neural network model was developed to recognize and classify chronic cervicitis and cervical cancer. A total of 10,012 colposcopy images of 1,081 patients from Hunan Provincial People's Hospital in China were recorded. Five different colposcopy image features of the cervix including chronic cervicitis, intraepithelial lesions, cancer, polypus, and free hyperplastic squamous epithelial tissue were extracted to be applied in our deep learning network convolutional neural network model. However, the result showed a low accuracy (42.16%) due to computer misrecognition of chronic cervicitis, intraepithelial lesions, and free hyperplastic squamous epithelial tissue with high similarity. To optimize this model, we selected two significant feature images: chronic cervicitis and cervical cancer to input into a deep learning network. The result indicates high accuracy and robustness with an accuracy of 95.19%, which can be applied to detect whether the patient has chronic cervicitis or cervical cancer based on the patient's colposcopy images.
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http://dx.doi.org/10.3389/fphar.2022.911962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196041PMC
May 2022

Dissecting the Heterogeneity of Human Thoracic Aortic Aneurysms Using Single-Cell Transcriptomics.

Arterioscler Thromb Vasc Biol 2022 Aug 16;42(8):919-930. Epub 2022 Jun 16.

Department of Cardiac Surgery, University of Michigan, Ann Arbor (D.M., H.F., B.Y.).

Thoracic aortic aneurysm is a life-threatening condition caused by weakening of the thoracic aorta wall, often developing silently until dissection or rupture occurs. Despite substantial efforts in the past decade, there have been no significant therapeutic advances to prevent or clinically manage diverse forms of thoracic aortic aneurysm and dissection with the only effective treatment being surgical repair. There is an urgent need to understand intra- and inter-aneurysmal heterogeneity underlying thoracic aortic aneurysm and dissection pathogenesis. The human aortic wall consists of many cell types and exhibits significant regional heterogeneity. High-throughput single-cell RNA sequencing has emerged as the principal tool to reveal the complexity in human tissues and clinical specimens. Recent single-cell RNA sequencing studies of different aortic cell populations both in vivo and in vitro began to dissect this complexity and have provided valuable information. In this review, we summarize these findings and discuss the potential applications of single-cell transcriptomics and related high-content technologies in human thoracic aortic aneurysm and dissection research, as well as the challenges associated with it.
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http://dx.doi.org/10.1161/ATVBAHA.122.317484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339526PMC
August 2022

The Impact of the COVID-19 Pandemic on China's Airline Industry.

Front Public Health 2022 27;10:865463. Epub 2022 May 27.

School of Economics, Tianjin University of Commerce, Tianjin, China.

Background: The COVID-19 pandemic has posed a great challenge to the development of China's airline industry. Although the existing literature has analyzed the economic impact of the pandemic on the airline industry from different perspectives, it remains to be further studied given the operating characteristics of different types of airlines in China.

Methods: Using a new perspective of heterogeneous airline service models, this study collects high-frequency data on stock prices on six sample airline companies (including both full-service airlines and low-cost airlines) in China over 519 trading days, from August 1, 2019 to September 15, 2021, and identifies structural change points for each company's stock price using the Quandt-Andrews test. The outcome is used to construct an econometric model to quantify the economic impact of the pandemic on different airlines' stock prices under different structural changes.

Results: All results have passed the Quandt-Andrews test. The impact coefficient of full-service airlines is negative, while that of low-cost airlines is positive. Most of them have passed the test at the significance level of 10%.

Conclusions: All Chinese airlines experienced significant sudden changes in stock prices due to the pandemic, but there are sectoral differences in the order of the sudden changes, with full-service airlines experiencing structural changes much earlier than low-cost airlines. In addition, the impact of the pandemic on stock prices varies across airline types, with a negative impact on full-service airlines and a significant positive effect on most low-cost airlines.
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http://dx.doi.org/10.3389/fpubh.2022.865463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184715PMC
May 2022

Relationship between Enterprise Financing Structure and Business Performance Assisted by Blockchain for Internet of Things Financing Mode.

Comput Intell Neurosci 2022 31;2022:2076830. Epub 2022 May 31.

Information Engineering Institute, Wuhan College, Wuhan 430212, Hubei, China.

Financing structure is an important and very complex issue in the financial theory and the rights and obligations of relevant stakeholders of enterprises are also concentrated in the financing structure. Therefore, the financing structure has a significant impact on the value of enterprises. A reasonable financing structure is conducive to standardizing the behavior of enterprises and improving the value of enterprises. The change of corporate financing structure is often used as a signal to external investors about the company's future income expectations, especially because the financing structure has a certain impact on the company's performance, which makes the problem of financing structure more valued by the theoretical and financial circles. For the empirical information about company financing, this paper explores the influencing elements of the company's running overall performance assisted with the aid of the blockchain, and the net of matters provides a chain model and constructs the operating performance indicators according to the comprehensive score. We select the commercial credit financing rate, short-term loan financing rate, long-term loan financing rate, debt financing rate, equity financing rate, and endogenous financing rate. The control variables are total capital, ownership concentration, and average age of the company. The conclusion is drawn by regression analysis. Commercial savings financing rate, fairness financing fee, and endogenous financing fee are positively correlated with the working performance; short-term loans and average age of the company are negatively correlated with the operating performance; and long-term loan financing rate, bond financing rate, and equity concentration are not significantly correlated with the operating performance.
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http://dx.doi.org/10.1155/2022/2076830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173942PMC
June 2022

Microbial Community Analysis and Food Safety Practice Survey-Based Hazard Identification and Risk Assessment for Controlled Environment Hydroponic/Aquaponic Farming Systems.

Front Microbiol 2022 19;13:879260. Epub 2022 May 19.

Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, United States.

Hydroponic and aquaponic farming is becoming increasingly popular as a solution to address global food security. Plants in hydroponic systems are grown hydroponically under controlled environments and are considered to have fewer food safety concerns than traditional field farming. However, hydroponics and aquaponics might have very different sources of microbial food safety risks that remain under-examined. In this study, we investigated the microbiomes, microbial hazards, and potential bacterial transmission routes inside two commercial hydroponic and aquaponic farming systems using 16S-ITS-23S rRNA sequencing and a hydroponic food safety practice survey. The hydroponic farming system microbiome was analyzed from the fresh produce, nutrient solution, tools, and farmworkers. Proteobacteria, Actinobacteria, Cyanobacteria, Bacteroidetes, and Firmicutes were the main components of hydroponic/aquaponic farming systems, with being the most abundant genus in fresh produce samples. We further identified the presence of multiple spoilage bacteria and potential human, plant, and fish pathogens at the subspecies level. Spoilage spp. and spoilage spp. were abundant in the hydroponic microgreen farm and aquaponic lettuce farm, respectively. Moreover, we demonstrated the mapping of 16s-ITS-23s rRNA sequence reads (∼2,500 bp) to small or large subunit rRNA databases and whole-genome databases to confirm pathogenicity and showed the potential of using 16s-ITS-23s rRNA sequencing for pathogen identification. With the SourceTracker and overlapping amplicon sequence variants, we predicted the bidirectional transmission route between plants and the surrounding environment and constructed the bacteria transmission map, which can be implemented in future food safety risk control plans.
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http://dx.doi.org/10.3389/fmicb.2022.879260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161294PMC
May 2022

[Establishment of a CFTR-based detection method for the second messenger cAMP in the cytoplasm].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2022 Jan;38(1):79-84

School of Basic Medicine, Jilin Medical College, Jilin 132013, China.

To establish a detection method based on Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) that can sensitively detect the second messenger cyclic AMP (cAMP) in the cytoplasm. The eukaryotic expression vectors of CFTR and YFP-H148Q / I152L were constructed respectively. FRT cells co-expressing CFTR and YFP-H148Q / I152L were obtained by liposome transfection. The expression of CFTR and YFP-H148Q / I152L in FRT cells was observed by an inverted fluorescence microscopy, and flow cytometry was used to detect the purity of cells; The cell model was identified by the fluorescence quenching kinetics test. The validation of the cell model which could screen CFTR modulators was verified by the fluorescence quenching kinetics experiments. The radioimmunoassay was used to detect the cAMP concentration in cytoplasm after adding CFTR activator. The results of the inverted fluorescence microscope showed that CFTR was expressed in the cell membrane and YFP-H148Q / I152L was expressed in the cytoplasm of FRT cells. The FRT cell model stably co-expressing ANO1 and YFP-H148Q / I152L was successfully constructed. The model could screen CFTR modulators, and the slope of fluorescence change and the concentration of CFTR modulators were in a dose-dependent manner. The slope of the fluorescence could reflect the cAMP concentration in the cytoplasm. The cell model could sensitively detect the intracellular cAMP concentration. The cell model could efficiently and sensitively detect the second messenger cAMP concentration in the cytoplasm, and it provided a simple and efficient method for the study of other targets associated cAMP signal.
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http://dx.doi.org/10.12047/j.cjap.6208.2022.015DOI Listing
January 2022

Waterborne Cr and Cr exposure disturbed the intestinal microbiota homeostasis in juvenile leopard coral grouper Plectropomus leopardus.

Ecotoxicol Environ Saf 2022 Jul 24;239:113653. Epub 2022 May 24.

State Key Laboratory of Marine Resource Utilization in South China Sea, School of Life Sciences, College of Marine Science, Hainan University, Haikou 570228, China. Electronic address:

Background: Chromium (Cr) mainly has two stable forms: Cr and Cr. Cr and its compound are widely used in the printing, dyeing, leather making, and metallurgy industry. They are evitably released into the environment and pose a significant threat to creatures, for instance, the excessive chromium (Cr) burden in the marine ecosystem is often harmful to fish. Intestinal microbiota greatly affects fish performance, but how waterborne Cr affects fish intestinal microbiota is unclear. To test the hypothesis that the waterborne Cr exposure could significantly affect fish' intestinal microbiota homeostasis, and the effect was highly dependent on Cr concentration and speciation, the juvenile leopard coral grouper Plectropomus leopardus were exposed to waterborne Cr and Cr (0.1, 0.5 ppm) for 7 days, and the intestinal microbiota was determined by Amplicon sequencing of the 16S rRNA.

Results: In all Cr treatment groups, the alpha diversity of intestinal microbiota communities of P. leopardus was decreased. The phyla Proteobacteria, Firmicutes, Actinobacteria, Chloroflexi and Bacteroidetes were the dominant intestinal microbiota. The Chao index diversity significantly declined in Cr treatment group, indicating the intestinal microbiota community structure was changed. Among the dominant intestinal microbiota, Proteobacteria was most sensitive to Cr exposure, and it increased after xposure. The PICRUSt predicted that 0.5 ppm Cr expousure caused metabolism disordered in the intestinal of P. leopardus.

Conclusions: Waterborne Cr and Cr significantly disturbed intestinal microbiota homeostasis in P. leopardus, including their diversity, composition, and community structure. The metabolism level of intestinal microbiota in P. leopardus was decreased by Cr exposure. High concentrations of Cr may pose potential risks to the intestinal homeostasis of P. leopardus.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113653DOI Listing
July 2022

Endoleak management and postoperative surveillance following endovascular repair of internal carotid artery vascular diseases using Willis covered stent.

J Interv Med 2021 Nov 23;4(4):212-218. Epub 2021 Dec 23.

Department of Interventional Radiology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

To report the clinical results and experiences of endoleak management and postoperative surveillance following endovascular repair of internal carotid artery vascular diseases (ICAVDs) using Willis covered stents.

Methods: Seventy-three patients with ICAVD who received Willis covered stent implantation between November 2013 and September 2018 were retrospectively reviewed. The clinical data of endoleak management and postoperative surveillance were analyzed.

Results: Seventy-three cases with ICAVD, including 57 aneurysms, 11 carotid-cavernous sinus fistulas (CCF), and 5 surgical injuries, were all successfully installed with covered stents. Total isolation of ICAVDs was achieved in 59 patients (80.8%), and endoleaks were observed in 14 patients (19.2%). Of the 14 patients with endoleaks, 12 had type I endoleaks and 2 had type II; 13 had aneurysms and one had CCF. 10 patients with type I endoleaks received balloon dilatation, and 7 of them underwent a second stent-graft implantation after then. One patient with type II endoleak received embolization of the branch artery, and another one received follow-up observation. Endoleaks resolved in 6 patients and were minimal in 5 patients after balloon dilatation or the second stent implantation. During the follow-up period, minor endoleaks spontaneously resolved in 4 patients and minimal endoleaks were still demonstrated in 4 patients without enlargement of residual lumen and rupture.

Conclusions: Endoleaks are the major complication after endovascular repair of ICAVDs and represent one of the limitations of this procedure. Improving the understanding and management of endoleaks can be beneficial in the clinical setting, including the popularization and application of this technique.
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http://dx.doi.org/10.1016/j.jimed.2021.09.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947986PMC
November 2021
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