Publications by authors named "Hao Cui"

318 Publications

Fractionation, source, and ecological risk assessment of heavy metals in cropland soils across a 100-year reclamation chronosequence in an estuary, south China.

Sci Total Environ 2021 Nov 22:151725. Epub 2021 Nov 22.

Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology, Shandong Academy of Sciences, Ji'nan 250103, PR China.

Coastal reclamation for cropland has led to the accumulation of heavy metals in soils, bringing about pervasive and severe risks for environment and human health. Less is known about the influence of long-term reclamation on heavy metals risk, mobility and bioavailability in cropland soil. In this study, we determined six heavy metals (Cd, Cr, Ni, Cu, Zn and Pb) and their fractionations in soils from five croplands across a 100-year reclamation chronosequence in the Pearl River estuary. Results showed that across five reclaimed soils, Cd poses seriously ecological risk and bioavailability according to assessments based on both total contents (single-metal pollution index: Cd > Cu > Zn > Ni > Cr > Pb) and fractionations (risk assessment code: Cd > Zn > Cu > Ni > Pb > Cr). Cr, Ni, Cu, Zn and Pb pose slightly to moderately ecological risks, and were mainly bound to residual (73.70%) fractions and reducible (15.86%) fractions with lower mobility and bioavailability. With the highest risks levels, mobility, toxicity and bioavailability (5.67% exchangeable and 11.75% carbonate fractions bound), Cd was identified as the main pollution factor in study area. Principal component analysis and Pearson's correlation analysis revealed that anthropogenic reclamation activities (including phosphate fertilizers, pesticides and sewage irrigation) were the major sources of these heavy metals. Long-term reclamation activities induced the increases of soil organic matter, clay contents, total concentrations and non-residual fractions of heavy metals by 46.14%, 538.98%, 42.87% and 219.78%, respectively, demonstrating significant promotions in level and mobility of heavy metals due to longer-term agricultural activities, higher soil clay and organic matter content.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151725DOI Listing
November 2021

Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019.

Poult Sci 2021 Oct 10;101(1):101522. Epub 2021 Oct 10.

College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.

Mycoplasma synoviae (M. synoviae) has been identified worldwide to cause respiratory diseases, infectious synovitis, airsacculitis, and eggshell apex abnormalities (EAA) in commercial chickens, which results in substantial economic losses to the poultry industry. Therefore, in this study, 258 flocks were investigated between 2017 and 2019 for M. synoviae by screening samples from Central China. Subsequently, 129 M. synoviae strains were isolated, with a positive rate of 50%. Moreover, a higher incidence of M. Synoviae infections was in layers (74.1%) than in broilers (20%) in this study. The 5'-end conserved segment of the variable lipoprotein hemagglutinin A (vlhA) gene of these isolates was then cloned and sequenced because it is a common genomic target identified so far for M. synoviae genotyping. Genotyping of all isolates was based on the phylogenetic analysis and length analysis of the proline-rich-repeat (PRR) regions, respectively. Phylogenetic analysis based on 5'-end conserved segment of the vlhA gene (76-421 nt) assigned the majority of the occurring strains as being from group 6, and others from groups 2 and 3. Results identified that these isolates were of 6 types: A (38aa), D (23aa), E (19aa), I (28aa), J (20aa), and L (35aa), based on the size of the PRR region analysis. Furthermore, most of the isolates (81.4% were identified as type L. Additionally, the epidemic types included only I and L in 2017; however, the types rose to 5 (A, D, E, I, L) in 2018 and rose to 6 (A, D, E, I, J, L) in 2019. These data showed the genotype diversity of M. synoviae in Central China. The high rate of positive flocks suggests the urgent need to take real-time supervisory controls of this Mycoplasma species in avian flocks.
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http://dx.doi.org/10.1016/j.psj.2021.101522DOI Listing
October 2021

Systematic investigation on the distribution of four hidden toxic Aconitum alkaloids in commonly used Aconitum herbs and their acute toxicity.

J Pharm Biomed Anal 2021 Nov 16;208:114471. Epub 2021 Nov 16.

Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macao 999078, PR China; Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, Macau University of Science and Technology, Macao 999078, PR China. Electronic address:

Yunaconitine (YAC), crassicauline A (CCA), 8-deacetylyunaconitine (DYA), and 8-deacetylcrassicauline A (DCA), as hidden toxic Aconitum alkaloids, are detected in some products of processed Aconitum carmichaelii lateral root and poisoning cases. The distribution and toxicity of these four components in Aconitum herbs should be further systematically studied for medication safety. This study developed a new UHPLC-QQQ-MS/MS method to determine ten Aconitum alkaloids, including aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine, benzoylhypaconine, YAC, CCA, DYA, and DCA, for Aconitum herbs simultaneously. YAC and CCA were founded in some samples of unprocessed A. carmichaelii lateral root (7.04%), A. carmichaelii root (9.43%), A. brachypodum root (6.00%), and A. ouvrardianum root (100%). Four hidden toxic Aconitum alkaloids were detected in processed A. carmichaelii lateral root (2.56%) and A. vilmorinianum root (100%). Four hidden toxic Aconitum alkaloids played significant roles in the classification of Aconitum herbs by OPLS-DA analysis. The acute toxicity test was performed by up-and-down procedure (UDP). The oral administration of the half lethal dose (LD) of YAC, CCA, DYA, and DCA to female ICR mice was 2.37 mg/kg, 5.60 mg/kg, 60.0 mg/kg, and 753 mg/kg, respectively. The LD by intravenous injection was 0.200 mg/kg, 0.980 mg/kg, 7.60 mg/kg, and 34.0 mg/kg, respectively. The LD of unprocessed A. carmichaelii lateral root, A. vilmorinianum root, and A. brachypodum root to mice orally was 1.89 g/kg, 0.950 g/kg, and 0.380 g/kg, respectively. Symptoms of Aconitum alkaloid poisoning in mice were decreased activity, fur erect, palpebral edema, vomiting, polypnea, and convulsions. The main change of organs was flatulence. No poisoning or death occurred in mice at the maximum dosage (27.0 g/kg) of A. ouvrardianum root orally. To better control the quality and safety of Aconitum herbs, this study provides favorable support for improving the existing standards to strengthen the supervision of the four hidden toxic Aconitum alkaloids.
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http://dx.doi.org/10.1016/j.jpba.2021.114471DOI Listing
November 2021

Aloe-emodin derivative produces anti-atherosclerosis effect by reinforcing AMBRA1-mediated endothelial autophagy.

Eur J Pharmacol 2021 Nov 17:174641. Epub 2021 Nov 17.

Department of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, PR China; Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, Harbin, PR China; Research Unit of Noninfectious Chronic Diseases in Frigid Zone, Chinese Academy of Medical Sciences, 2019RU070, PR China. Electronic address:

Atherosclerosis is an inflammatory disease of high lethality associated with endothelial dysfunction. Due to the pathophysiological complexity and our incomplete understanding of the mechanisms for the development and progression of atherosclerosis, effective means for the prevention and treatment of atherosclerosis still need further exploration. This study was designed to investigate the potential effects and underlying mechanisms of aloe-emodin derivative (AED) on atherosclerosis. High fat diet (HFD) treated ApoE mice were used as an animal model of atherosclerosis. Intragastric administration of aloe-emodin (AE) or AED for 12 weeks markedly reduced the atherosclerotic plaque in aorta with decreased plaque area, lipid accumulation, macrophage infiltration, collagen content and metabolic abnormalities. By comparison, AED produced more potent anti-atherosclerosis effects than AE at the same dose. AED enhanced production of autophagy flux in cultured human aortic endothelial cells (HAECs). Moreover, AED increased the expression of activating molecule in Beclin1-regulated autophagy 1 (AMBRA1), a key protein involved in autophagosome formation. Furthermore, knockdown of AMBRA1 blocked the promotion effect of AED on autophagy in HAECs. Taken together, AED facilitates endothelial autophagy via AMBRA1 during the progression of atherosclerosis, suggesting the potential application of this compound for atherosclerosis treatment.
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http://dx.doi.org/10.1016/j.ejphar.2021.174641DOI Listing
November 2021

InP Monolayer as a Promising 2D Sensing Material in SF Insulation Devices.

ACS Omega 2021 Nov 25;6(44):29752-29758. Epub 2021 Oct 25.

State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing 400044, China.

In this letter, we perform a first-principles study on the adsorption performance of the InP monolayer upon three SF decomposed species, including SO, SOF, and SOF, to investigate its potential as a resistance-type, optical or field-effect transistor gas sensor. Results indicate that the InP monolayer exhibits strong chemisorption upon SO but weak physisorption upon SOF. The most admirable adsorption behavior is upon SOF, which provides a favorable sensing response (-19.4%) and recovery property (10.4 s) at room temperature as a resistance-type gas sensor. A high response of 180.7% upon SO and a poor one of -1.9% upon SOF are also identified, which reveals the feasibility of the InP monolayer as a resistance-type sensor for SO detection with recycle use via a heating technique to clean the surface. Moreover, the InP monolayer is a promising optical sensor for SO detection due to the obvious changes in adsorption peaks within the range of ultraviolet and is a desirable field-effect transistor sensor for selective and sensitive detection of SO and SOF given the evident changes of and under the applied electric field.
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http://dx.doi.org/10.1021/acsomega.1c04185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582028PMC
November 2021

Preoperative left ventricular longitudinal strain predicts outcome of septal myectomy for obstructive hypertrophic cardiomyopathy.

J Thorac Cardiovasc Surg 2021 Oct 6. Epub 2021 Oct 6.

Department of Cardiovascular Medicine, Mayo Clinic, Rochester, Minn.

Objective: The objective of this study was to determine the characteristics of longitudinal strain and its effect on outcomes in patients with obstructive hypertrophic cardiomyopathy (HCM) who underwent septal myectomy.

Methods: We reviewed patients with obstructive HCM who underwent septal myectomy at our clinic from 2007 to 2016. Data of those who had strain echocardiography within 6 months before isolated myectomy were analyzed.

Results: The median age of the 857 patients studied was 55 (interquartile range [IQR], 44-63) years, and 451 (52.6%) were male. Left ventricular ejection fraction was 71% (IQR, 67%-74%), and the resting peak outflow tract gradient was 58 (IQR, 27-85) mm Hg. The median global longitudinal strain (GLS) was -14.6% (IQR, -12.0% to -17.3%). Regional longitudinal strain was nonuniform as reflected by more normal values in apical segments and more abnormal in basal segments. Moreover, GLS correlated poorly with ejection fraction and outflow tract gradient. In 64 patients who had postoperative strain echocardiography, GLS was comparable before and after septal myectomy, but regional strain was more uniform after myectomy. Over a follow-up of 8.3 (IQR, 6.5-10.3) years, when patients were equally stratified according to GLS (cutoff, -14.64%), the group with worse GLS had significantly poorer survival compared with the better GLS group (P = .002). Left ventricular ejection fraction had no association with survival.

Conclusions: Left ventricular longitudinal strain is nonuniform and might be significantly reduced in patients with obstructive HCM. Septal myectomy does not impair GLS but is associated with more uniform regional strains. Most importantly, reduced GLS preoperatively is strongly and independently associated with increased all-cause mortality after septal myectomy for obstructive HCM.
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http://dx.doi.org/10.1016/j.jtcvs.2021.09.058DOI Listing
October 2021

Systems biomarker characteristics of circulating alkaline phosphatase activities for 48 types of human diseases.

Curr Med Res Opin 2021 Nov 12:1-9. Epub 2021 Nov 12.

Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao, China.

Background: Most human diseases are accompanied by systems changes. Systems biomarkers should reflect such changes. The phosphorylation and dephosphorylation of biomolecules maintain human homeostasis. However, the systems biomarker characteristics of circulating alkaline phosphatase, a routine blood test conducted for many human diseases, have never been investigated.

Method: This study retrieved the circulating alkaline phosphatase (ALP) activities from patients with 48 clinically confirmed diseases and healthy individuals from the database of our hospital during the past five years. A detailed analysis of the statistical characteristics of ALP was conducted, including quantiles, receiving operator curve (ROC), and principal component analysis.

Results: Among the 48 diseases, 45 had increased, and three had decreased median levels of ALP activities compared to the healthy control. Preeclampsia, hepatic encephalopathy, pancreatic cancer, and liver cancer had the highest median values, whereas nephrotic syndrome, lupus erythematosus, and nephritis had decreased median values compared to the healthy control. Further, area under curve (AUC) values were ranged between 0.61 and 0.87 for 19 diseases, and the ALP activities were the best systems biomarker for preeclampsia (AUC 0.87), hepatic encephalopathy (AUC 0.87), liver cancer (AUC 0.81), and pancreatic cancer (AUC 0.81).

Conclusions: Alkaline phosphatase was a decent systems biomarker for 19 different types of human diseases. Understanding the molecular mechanisms of over-up-and-down-regulation of ALP activities might be the key to understanding the whole-body systems' reactions during specific disease progression.
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http://dx.doi.org/10.1080/03007995.2021.2000715DOI Listing
November 2021

Plasma Metabolites-Based Prediction in Cardiac Surgery-Associated Acute Kidney Injury.

J Am Heart Assoc 2021 Nov 30;10(22):e021825. Epub 2021 Oct 30.

The Cardiomyopathy Research Group State Key Laboratory of Cardiovascular Disease Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical College Beijing China.

Background Cardiac surgery-associated acute kidney injury (CSA-AKI) is a common postoperative complication following cardiac surgery. Currently, there are no reliable methods for the early prediction of CSA-AKI in hospitalized patients. This study developed and evaluated the diagnostic use of metabolomics-based biomarkers in patients with CSA-AKI. Methods and Results A total of 214 individuals (122 patients with acute kidney injury [AKI], 92 patients without AKI as controls) were enrolled in this study. Plasma samples were analyzed by liquid chromatography tandem mass spectrometry using untargeted and targeted metabolomic approaches. Time-dependent effects of selected metabolites were investigated in an AKI swine model. Multiple machine learning algorithms were used to identify plasma metabolites positively associated with CSA-AKI. Metabolomic analyses from plasma samples taken within 24 hours following cardiac surgery were useful for distinguishing patients with AKI from controls without AKI. Gluconic acid, fumaric acid, and pseudouridine were significantly upregulated in patients with AKI. A random forest model constructed with selected clinical parameters and metabolites exhibited excellent discriminative ability (area under curve, 0.939; 95% CI, 0.879-0.998). In the AKI swine model, plasma levels of the 3 discriminating metabolites increased in a time-dependent manner (, 0.480-0.945). Use of this AKI predictive model was then confirmed in the validation cohort (area under curve, 0.972; 95% CI, 0.947-0.996). The predictive model remained robust when tested in a subset of patients with early-stage AKI in the validation cohort (area under curve, 0.943; 95% CI, 0.883-1.000). Conclusions High-resolution metabolomics is sufficiently powerful for developing novel biomarkers. Plasma levels of 3 metabolites were useful for the early identification of CSA-AKI.
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http://dx.doi.org/10.1161/JAHA.121.021825DOI Listing
November 2021

Lung metastasis and lymph node metastasis are risk factors for hyperprogressive disease in primary liver cancer patients treated with immune checkpoint inhibitors.

Ann Palliat Med 2021 Oct 11. Epub 2021 Oct 11.

Big Data Center, Nanfang Hospital, Southern Medical University, Guangzhou, China; Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: At present, some cancer patients experience hyperprogressive disease (HPD) after receiving immunotherapy. This study used the Response Evaluation Criteria in Solid Tumors 1.1 to evaluate the incidence of HPD in patients receiving immune checkpoint inhibitors (ICIs) for treating primary liver cancer (PLC) and to explore the risk factors for HPD.

Methods: This retrospective, single-center study included patients with PLC who were treated with ICIs. The RECIST 1.1 was used to determine patients with HPD. Univariate and multivariate regression analyses were performed to explore the risk factors for HPD, and clinical variables with prognostic significance for HPD were included to establish a risk model.

Results: Among 129 patients with PLC treated with ICIs, HPD occurred in 13 patients (10.1%). In the multivariate regression analysis, lymph node metastasis and lung metastasis were risk factors for HPD. The area under the curve of the risk model, established by including lymph node metastasis, lung metastasis, neutrophil-lymphocyte ratio, albumin, and performance status, was 0.801 (P<0.001). The progression-free survival of HPD patients was significantly worse than that of non-HPD patients (P<0.001).

Conclusions: In this study, 10.1% of patients with PLC had HPD. Compared with the non-HPD patients, lung metastasis and lymph node metastasis were independent risk factors of HPD.
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http://dx.doi.org/10.21037/apm-21-2023DOI Listing
October 2021

Efficacy of Qingfei Paidu Decoction on Patients with COVID-19 Pneumonia in Wuhan, China: A Propensity Score Matching Study.

Evid Based Complement Alternat Med 2021 5;2021:4303380. Epub 2021 Oct 5.

Department of Emergency Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: In view of the global efforts to develop effective treatments for the current worldwide coronavirus 2019 (COVID-19) pandemic, Qingfei Paidu decoction (QPD), a novel traditional Chinese medicine (TCM) prescription, was formulated as an optimized combination of constituents of classic prescriptions used to treat numerous febrile and respiratory-related diseases. This prescription has been used to treat patients with COVID-19 pneumonia in Wuhan, China. . We hypothesized that QPD would have beneficial effects on patients with COVID-19. We aimed to prove this hypothesis by evaluating the efficacy of QPD in patients with COVID-19 pneumonia.

Methods: In this single-center, retrospective, observational study, we identified eligible participants who received a laboratory diagnosis of COVID-19 between January 15 and March 15, 2020, in the west campus of Union Hospital in Wuhan, China. QPD was supplied as an oral liquid packaged in 200-mL containers, and patients were orally administered one package twice daily 40 minutes after a meal. The primary outcome was death, which was compared between patients who did and did not receive QPD (QPD and NoQPD groups, respectively). Propensity score matching (PSM) was used to identify cohorts.

Results: In total, 239 and 522 participants were enrolled in the QPD and NoQPD groups, respectively. After PSM at a 1 : 1 ratio, 446 patients meeting the criteria were included in the analysis with 223 in each arm. In the QPD and NoQPD groups, 7 (3.2%) and 29 (13.0%) patients died, and those in the QPD group had a significantly lower risk of death (hazard ratio (HR) 0.29, 95% CI: 0.13-0.67) than those in the NoQPD group ( = 0.004). Furthermore, the survival time was significantly longer in the QPD group than in the NoQPD group ( < 0.001).

Conclusion: The use of QPD may reduce the risk of death in patients with COVID-19 pneumonia.
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http://dx.doi.org/10.1155/2021/4303380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510827PMC
October 2021

Simultaneously Blocking Chemical Crosstalk and Internal Short Circuit via Gel-Stretching Derived Nanoporous Non-Shrinkage Separator for Safe Lithium-Ion Batteries.

Adv Mater 2021 Oct 7:e2106335. Epub 2021 Oct 7.

Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.

The separator, an ionic permeable and electronic insulating membrane between cathode and anode, plays a crucial role in the electrochemical and safety performance of batteries. However, commercial polyolefin separators not only suffer from inevitable thermal shrinkage at elevated temperature, but also fail to inhibit the hidden chemical crosstalk of reactive gases such as O , leading to often reported thermal runaway (TR) and hence preventing large-scale implementation of high-energy-density lithium-ion batteries. Herein, a nanoporous non-shrinkage separator (GS-PI) is fabricated via a novel gel-stretching orientation approach to eliminate TR. In situ synchrotron small angle X-ray scattering during heating clearly shows that the as-prepared thin GS-PI separator exhibits superior mechanical tolerance at high temperature, thus effectively preventing internal short circuit. Meanwhile, the unique nanoporous structure design further blocks chemical crosstalk and the associated exothermic reactions. Accelerating rate calorimetry tests reveal that the practical 1 Ah LiNi Co Mn O (NCM622)/graphite pouch cell using GS-PI nanoporous separator show a maximum temperature rise (dT/dt ) of only 3.7 °C s compared to 131.6 °C s in the case of Al O @PE macroporous separator. Moreover, despite the reduced pore size, the GS-PI separator demonstrates better cycling stability than conventional Al O @PE separator at high temperature without sacrificing specific capacity and rate capability.
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http://dx.doi.org/10.1002/adma.202106335DOI Listing
October 2021

Impact of septal myectomy on diastolic function in patients with obstructive hypertrophic cardiomyopathy.

J Thorac Dis 2021 Aug;13(8):4925-4934

Department of Cardiovascular Surgery, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The impact of septal myectomy on diastolic function in patients with obstructive hypertrophic cardiomyopathy is not well studied.

Methods: A transcatheter hemodynamic study was performed before and 3 to 6 months after septal myectomy in 12 patients with obstructive hypertrophic cardiomyopathy (HCM).

Results: Postoperative hemodynamic studies were done 4.4±1.2 months after myectomy. The left ventricular outflow tract peak-to-peak gradient decreased from 83.2±43.3 mmHg preoperatively to 11.6±4.3 mmHg after myectomy (P<0.00). The left ventricular diastolic time constant (Tau) was 64.2±26.1 ms before surgery and 42.2±15.7 ms postoperatively (P=0.029). The average left atrial pressure (LAP) decreased from 20.2±7.0 to 12.1±4.5 mmHg after myectomy (P=0.008). Pulmonary artery hypertension was present in 6 patients preoperatively and remained in 2 patients after myectomy. Mean pulmonary artery pressure decreased from 29.3±16.2 to 20±6.7 mmHg after surgery (P=0.05), and the systolic pulmonary artery pressure decreased from 46±26.9 to 30.5±8.3 mmHg (P=0.048). Pulmonary vascular resistance decreased from 5.7±4.1 to 3.6±1.6 wood after surgery (P=0.032).

Conclusions: Septal myectomy improved left ventricular diastolic function and subsequently relieved the right ventricular congestion in patients with obstructive HCM.
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http://dx.doi.org/10.21037/jtd-21-902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411175PMC
August 2021

Knockdown of PGM1 enhances anticancer effects of orlistat in gastric cancer under glucose deprivation.

Cancer Cell Int 2021 Sep 10;21(1):481. Epub 2021 Sep 10.

Medical School of Chinese PLA, Beijing, 100853, China.

Background: Phosphoglucomutase 1 (PGM1) acts as an important regulator in glucose metabolism. However, the role of PGM1 in gastric cancer (GC) remains unclear. This study aims to investigate the role of PGM1 and develop novel regimens based on metabolic reprogramming in GC.

Methods: Correlation and enrichment analyses of PGM1 were conducted based on The Cancer Genome Atlas database. Data derived from the Kaplan-Meier Plotter database were analyzed to evaluate correlations between PGM1 expression and survival time of GC patients. Cell counting kit-8, 5-Ethynyl-2-deoxyuridine, flow cytometry assays, generation of subcutaneous tumor and lung metastasis mouse models were used to determine growth and metastasis in vitro and in vivo. Cell glycolysis was detected by a battery of glycolytic indicators, including lactate, pyruvic acid, ATP production and glucose uptake. Fatty Acid Synthase (FASN) activity and expression levels of lipid enzymes were determined to reflect on lipid metabolism.

Results: Correlation and enrichment analyses suggested that PGM1 was closely associated with cell viability, proliferation and metabolism. PGM1 was overexpressed in GC tissues and cell lines. High PGM1 expression served as an indicator of shorter survival for specific subpopulation of GC patients. It was also correlated with pathological tumor stage and pathological tumor node metastasis stage of GC. Under the glucose deprivation condition, knockdown of PGM1 significantly suppressed cell viability, proliferation and glycolysis, whereas lipid metabolism was enhanced. Orlistat, as a drug that was designed to inhibit FASN activity, effectively induced apoptosis and suppressed lipid metabolism in GC. However, orlistat conversely increased glycolytic levels. Orlistat exhibited more significant inhibitive effects on GC progression after knockdown of PGM1 under glucose deprivation due to combination of glycolysis and lipid metabolism both in vitro and in vivo.

Conclusions: Downregulation of PGM1 expression under glucose deprivation enhanced anti-cancer effects of orlistat. This combination application may serve as a novel strategy for GC treatment.
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http://dx.doi.org/10.1186/s12935-021-02193-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434706PMC
September 2021

Indium sulfide deposited MIL-53(Fe) microrods: Efficient visible-light-driven photocatalytic reduction of hexavalent chromium.

J Colloid Interface Sci 2022 Jan 18;606(Pt 2):1299-1310. Epub 2021 Aug 18.

School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.

The ecosystems and human health were seriously threatened by hexavalent chromium (Cr(VI)) in wastewater. In this article, using the idea of the highly matched energy band structure between indium sulfide (InS) and MIL-53(Fe), a Type-II heterojunction has been constructed by loading InS on MIL-53(Fe) microrod to overcome the fault like high recombination rates of photogenerated electron-holes of InS. The composite with 20:1 mass ratio of InS to MIL-53(Fe) (IM-2) was adopted as an optimal sample for efficient photocatalytic Cr(VI) reduction under visible light. Various characterization techniques were used to verify the characteristics of composites and delved into the structure-effect relationship between this heterojunction and its activity. Results showed that the reaction rate constants of the photoreduction process over IM-2 was ~ 4 and 26 times higher than those of pure InS and MIL-53(Fe), respectively, and the catalyst could maintain superior removal efficiency (88.6%) and steady crystal structure after four cycles. First-principles calculations further illustrated that the heterostructure formed between InS and MIL-53(Fe) could effectively accelerate the separation of photogenerated electrons and holes, thus improving the photocatalytic reduction performance. Moreover, the active species analyses revealed that the superoxide radicals and electrons were mainly involved in the reduction of Cr(VI).
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http://dx.doi.org/10.1016/j.jcis.2021.08.111DOI Listing
January 2022

Shift of microbial turnover time and metabolic efficiency strongly regulates rhizosphere priming effect under nitrogen fertilization in paddy soil.

Sci Total Environ 2021 Dec 10;800:149590. Epub 2021 Aug 10.

Institute of Environment Pollution Control and Treatment, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China. Electronic address:

Microbial turnover and the decomposition of soil organic matter can be stimulated by living roots in a phenomenon known as the rhizosphere priming effect (RPE). Both the microbial turnover time (MTT) and metabolic efficiency are closely related to RPE. However, changes in MTT, metabolic efficiency and RPE in response to nitrogen (N) fertilization at different levels and the associations between these factors during plant growth are unknown. The effects of N fertilization at different levels (0, 150 and 300 kg N ha) on RPE and the underlying mechanisms were investigated in maize (Zea mays L.) grown in paddy soil using a Carbon (C) natural abundance method. The RPE varied from -1.49 to 15.93 mg C kg soil day, with significant effects at different levels of N fertilization, growth stages and interactions between these factors. Nitrogen fertilization reduced microbial C:N imbalance and soil pH. During the plant growth periods, the RPE was initially low because the microbes preferentially utilized plant-derived C, but later increased due to trade-offs between microbial N acquisition and acidity stress alleviation under N fertilization. The soil microbes altered their MTT and metabolic efficiency with changes in the microbial community structure to maintain stoichiometric homeostasis and adapt to acidity stress. RPE was lowest whereas MTT and metabolic efficiency were highest with N fertilization at 150 kg N ha. Changes in MTT and metabolic efficiency explained 84.5% of the variations in the RPE, and the latter had greater impact (55.8%) than the former (28.7%). Changes in MTT and metabolic efficiency to cope with microbial resource acquisition and acidity stress under N fertilization represent an important pathway for RPE regulation in paddy soil. These findings highlight the significance of MTT and metabolic efficiency in RPE regulation for optimization of the N fertilization level to mitigate soil C losses.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149590DOI Listing
December 2021

Presence of powdered activated carbon/zeolite layer on the performances of gravity-driven membrane (GDM) system for drinking water treatment: Ammonia removal and flux stabilization.

Sci Total Environ 2021 Dec 2;799:149415. Epub 2021 Aug 2.

State Key Laboratory of Urban Water Resource and Environment (SKLUWRE), School of Environment, Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, PR China.

Gravity-driven membrane (GDM) filtration is a promising alternative for decentralized water supply, while its widespread application was hindered by the poor removals of organics and ammonia during long-term operation. In this study, powered activated carbon (PAC) and granular zeolite were selected as typical adsorbents to investigate the impacts of pre-deposited adsorbent layers on contaminant removal and membrane fouling. Results showed that the pre-deposited PAC layers exhibited higher removal of organics than the control, while the zeolites deposited layers exhibited low removal of organics. The presence of PAC only enhanced the NH removal at subsequent stable stage, while zeolites were effective in deal with sudden high NH concentration due to ion exchange. The presence of mixed adsorbents layers had similar organic removal with PAC and NH removal with zeolite. The pre-deposited PAC layers could effectively alleviate membrane fouling in short-term UF tests, while the stable fluxes (5.88-6.54 L/(m·h)) in long-term GDM operation were slightly lower than the control (6.63 L/(m·h)). The zeolites deposited layer aggravated membrane fouling in both short-term ultrafiltration and long-term GDM (5.03-3.84 L/(m·h)), but a higher stable flux (6.10 L/(m·h)) was observed for GDM using the mixed adsorbents. The pre-deposited adsorbent layers resulted in increased concentrations of biomass, tri-phosphate (ATP) and extracellular polymeric substances (EPS), forming cake layers with a denser structure than the control. Finally, the fouling mechanism for GDM using different adsorbent layers was proposed based on fouling analysis and characteristics of biological fouling layer. The results and conclusion in this study could provide helpful information for the application of GDM with pre-deposited adsorbent layer in treating raw water with organics and/or sudden high ammonia concentration to produce potable water.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149415DOI Listing
December 2021

Models for Predicting Sentinel and Non-sentinel Lymph Nodes Based on Pre-operative Ultrasonic Breast Imaging to Optimize Axillary Strategies.

Ultrasound Med Biol 2021 Nov 3;47(11):3101-3110. Epub 2021 Aug 3.

Department of Ultrasound, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. Electronic address:

Axillary strategy decisions have become more complex and controversial in considering minimally traumatic therapy instead of sentinel lymph node biopsy, axillary lymph node dissection or regional nodal irradiation for people with breast cancer. The purpose of this study was to noninvasively predict sentinel lymph node (SLN) and non-sentinel lymph node (NSLN) status based on pre-operative sonographic and clinicopathologic features to determine optimal decisions regarding axillary therapy. In total, 701 patients with breast cancer from two independent centers were retrospectively analyzed. The SLN model (SLNM) for predicting SLN status and the NSLN model (NSLNM) for predicting NSLN status were trained based on a training set using the random-forest algorithm, and their performance was validated using an independent external test set. A receiver operating characteristic curve was drawn to obtain the area under the curve, which was used to assess performance. The area under the curve for the SLNM in the training and test, respectively, was 94.2% and 83.0%, and for the NSLNM, 99.5% and 92.7%. The SLNM and NSLNM accurately predicted that 61.46% (319/519) and 17.53% (91/519), respectively, of our participants were non-metastatic. The overall benefit of the three models was 78.99% in our participants. The two models for predicting SLN and NSLN status showed excellent application potential in optimizing axillary strategies.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2021.06.014DOI Listing
November 2021

Aromatic Cadinane Sesquiterpenoids from the Fruiting Bodies of Block SARS-CoV-2 Spike-ACE2 Interaction.

J Nat Prod 2021 08 5;84(8):2385-2389. Epub 2021 Aug 5.

National R&D Center for Freshwater Fish Processing, and Engineering Research Center of Freshwater Fish High-Value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.

The ongoing COVID-19 global pandemic caused by SARS-CoV-2 inspires the development of effective inhibitors to block the SARS-CoV-2 spike-ACE2 interaction. A chemical investigation on the fruiting bodies of led to the isolation of five aromatic cadinane sesquiterpenoids including four new ones, named piniterpenoids A-D (-), as well as three known lignans. Their structures were determined by extensive spectroscopic analysis including HRMS and 1D and 2D NMR. All of the aromatic cadinane sesquiterpenoids inhibited the SARS-CoV-2 spike-ACE2 interaction, with IC values ranging from 64.5 to 99.1 μM. A molecular docking study showed the disruption of the interaction of compound via hydrogen interactions with Arg403, Asp405, and Arg408 of SARS-CoV-2 RBD and Arg393 and His34 residues of ACE2. These results suggested that aromatic cadinane sesquiterpenoids might be useful in developing agents for COVID-19.
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http://dx.doi.org/10.1021/acs.jnatprod.1c00426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353988PMC
August 2021

Liver-Targeted Delivery of Oligonucleotides with N-Acetylgalactosamine Conjugation.

ACS Omega 2021 Jun 14;6(25):16259-16265. Epub 2021 Jun 14.

GlycoNovo Technologies Co., Ltd., Shanghai 201203, People's Republic of China.

The potential therapeutic application of oligonucleotides (ONs) that selectively suppress target genes through antisense and RNA interference mechanisms has attracted great attention. The clinical applications of ONs have overcome multiple obstacles and become one of the most active areas for the development of novel therapeutics. To achieve efficient and specific cellular internalization, conjugation of a variety of functional groups to ONs has been the subject of intensive investigations over the past decade. Among them, a promising liver-targeted -acetylgalactosamine (GalNAc) ligand has been evaluated in multiple preclinical and clinical trials for improving the cellular uptake and tissue specific delivery of ONs. GalNAc-based delivery relies on the fact that liver hepatocytes abundantly and specifically express the asialoglycoprotein receptor that binds and uptakes circulating glycoproteins via receptor-mediated endocytosis. In recent years, encouraging progress has been made in the field of GalNAc conjugates. This review aims to provide an overview of GalNAc-mediated liver-targeted delivery of small interfering RNA and antisense oligonucleotides, and the immense effort as well as recent advances in the development of GalNAc-conjugated agents are described.
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http://dx.doi.org/10.1021/acsomega.1c01755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246477PMC
June 2021

Sleep Disordered Breathing and Mortality in Patients With Hypertrophic Obstructive Cardiomyopathy Undergoing Septal Myectomy.

Mayo Clin Proc 2021 09 3;96(9):2323-2331. Epub 2021 Jul 3.

Department of Cardiovascular Medicine, Mayo Clinic, Rochester, MN.

Objective: To determine the impact of sleep-disordered breathing (SDB) on survival in patients with hypertrophic cardiomyopathy (HCM) following septal myectomy.

Patients And Methods: Patients with obstructive HCM undergoing septal myectomy from 2007 to 2016 were reviewed. Those who had an overnight oximetry test within 6 months before myectomy were included in analysis. Oxygen desaturation index was examined continuously and also categorically (SDB [>5/h] and severe SDB [>15/h]).

Results: A total of 619 of 1500 patients undergoing septal myectomy had overnight oximetry tests. Sleep-disordered breathing (oxygen desaturation index >5/h) was identified in 338 (54.6%) patients, and among those patients, 117 (18.9%) were classified as severe. Patients with SDB were older, had greater body mass index and body surface area, were more likely to have arterial hypertension and atrial fibrillation, and had an increased E/e' ratio on Doppler echocardiography. Notably, there was no difference in preoperative resting left ventricular outflow tract pressure gradient between patients with SDB and those with normal overnight oximetry (55 (interquartile range: 25 to 86) mm Hg versus 52 (interquartile range: 21 to 85) mm Hg; P=.29). There was no difference in age-adjusted survival among patients with normal oximetry compared with those with mild SDB (hazard ratio: 0.98; 95% CI: 0.45 to 2.17), and severe SDB (hazard ratio: 1.06, 95% CI 0.42 - 2.71).

Conclusion: Sleep-disordered breathing is present in more than half of patients with obstructive HCM in whom septal myectomy is indicated, and is mainly associated with aging, overweight, and male sex. However, SDB does not alter survival following septal myectomy.
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http://dx.doi.org/10.1016/j.mayocp.2021.03.036DOI Listing
September 2021

Metabolic profile of heart tissue in cyanotic congenital heart disease.

Am J Transl Res 2021 15;13(5):4224-4232. Epub 2021 May 15.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College 167A Beilishi Road, Xi Cheng District, Beijing 100037, China.

Background: Cyanotic congenital heart disease (CCHD) is one of the most common birth anomalies, in which chronic hypoxia is the basic pathophysiological process.

Methods: To investigate the heart's metabolic remodeling to hypoxia, we performed an untargeted metabolomic analysis of cardiac tissue from 20 CCHD patients and 15 patients with acyanotic congenital heart disease (ACHD).

Results: A total of 71 (63%) metabolites from 113 detected substances in cardiac tissue differed between the CCHD and ACHD groups. A partial least squares discriminant analysis showed separation between the CCHD and ACHD groups. A pathway enrichment analysis revealed that the most enriched metabolic pathways were amino acid metabolism and energy metabolism. Eleven amino acids were increased in CCHD patients, indicating that protein synthesis was down-regulated. Most of the metabolites in Krebs circle were increased in CCHD patients, suggesting down regulation of aerobic energy metabolism. Hierarchical cluster analysis showed that nicotinamide adenine dinucleotide (NAD) was clustered with Krebs cycle related substrates and its level was significantly higher in CCHD than that in ACHD patients. These analyses suggest that NAD might play an important role in response to hypoxia in CCHD patients.

Conclusion: Our data showed a significantly different metabolic profile in CCHD patients compared to ACHD patients, including reduced protein synthesis and aerobic energy production, and the increased level of NAD in the myocardium may be a response mechanism to hypoxia.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205768PMC
May 2021

Could neoadjuvant chemotherapy increase postoperative complication risk of laparoscopic total gastrectomy? A mono-institutional propensity score-matched study in China.

World J Gastrointest Surg 2021 May;13(5):429-442

Department of General Surgery, Institute of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.

Background: The potential survival benefit of neoadjuvant chemotherapy (NC) in patients with advanced gastric cancer has been widely recognized. With the development of minimally invasive surgery, which is represented by laparoscopy, the effect of NC on the safety of laparoscopic gastrectomy remains to be further explored.

Aim: To compare the short-term outcomes of laparoscopic total gastrectomy (LTG) after NC (NC-LTG) with LTG alone.

Methods: A total of 92 patients who underwent NC-LTG and 381 patients who received LTG alone at the Chinese PLA General Hospital between September 2015 and September 2020 were retrospectively included in our study. We used propensity-score matching (PSM) to balance baseline bias. After 1:1 PSM, 73 patients were included in each group with no statistically significant difference in baseline characteristics.

Results: The NC-LTG group exhibited a longer operation time (244.10 ± 48.13 min 225.74 ± 45.33 min, = 0.019) and increased intraoperative blood loss [150 (100-300) mL 100 (100-200) mL, = 0.011] compared to the LTG group. The 30-d postoperative morbidity of the NC-LTG group was 20.5% (15/73), and that of the LTG group was 13.7% (10/73). There were no significant differences in 30-d severe complication rates or anastomotic leakage rates. Subgroup analysis showed that the patients with pTNM (pathological tumor-node-metastasis classification) T0N0-II in the NC-LTG group underwent a longer operation than the LTG group, while no significant difference was found in any perioperative index for the pTNM III patients. A multivariate analysis showed that an operation time longer than 240 min was an independent risk factor (odds ratio = 3.021, 95% confidence interval: 1.160-7.868, = 0.024), while NC was not an independent risk factor for postoperative complications in LTG.

Conclusion: Despite a longer operation time and more blood loss after NC-LTG, which indicate surgical difficulty, NC-LTG exhibits acceptable short-term outcomes compared to LTG, suggesting the safety and feasibility of NC-LTG.
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http://dx.doi.org/10.4240/wjgs.v13.i5.429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167844PMC
May 2021

Improving the Stability of α-CsPbI Nanocrystals in Extreme Conditions Facilitated by Mn Doping.

ACS Omega 2021 Jun 20;6(21):13831-13838. Epub 2021 May 20.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications (NUPT), 9 Wenyuan Road, Nanjing 210023, P. R. China.

The wide application of CsPbI nanocrystals (NCs) is limited due to their poor phase stability. We reported that Mn-CsPbI NCs have better optical performance and phase stability. With a suitable Mn/Pb ratio (5.0%), Mn-doped α-CsPbI NCs exhibited the best stability under UV irradiation, ethanol addition, and heating. Under UV irradiation and addition of ethanol, photoluminescence (PL) intensities of CsPbI NCs could be only preserved up to 35% (22 min UV irradiation) and 10% (ethanol addition), respectively, whereas, Mn-doped CsPbI (5.0%) exhibited much improved stability, and their intensities could be preserved up to 70% (22 min UV) and 58% (ethanol), respectively. It should be noted that crystal-phase stability could be maintained at least 7 h even at 120 °C. We believe that the improved stability in extreme conditions for α-CsPbI NCs can be further applied to optoelectronic devices.
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http://dx.doi.org/10.1021/acsomega.1c01383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173561PMC
June 2021

Essential Oils from cv. Shatangju Peel: Optimization of Hydrodistillation Extraction by Response Surface Methodology and Evaluation of Their Specific Adhesive Effect to Polystyrene.

ACS Omega 2021 Jun 19;6(21):13695-13703. Epub 2021 May 19.

College of Biology & Food Engineering, Jilin Institute of Chemical Technology, 45 Chengde Street, Longtan District, Jilin City 132002, China.

Essential oil (Eo) from the peel has been widely used, and its adhesive effect on polystyrene (PS) was found accidentally. To analyze the essential oils of cv. Shatangju (CrspEos), the extraction of these oils by steam distillation was optimized using the response surface method. The chemical composition of CrspEos was analyzed by gas chromatography-mass spectrometry. Then, the adhesive effect of these essential oils on PS was evaluated. The adhesion area, the influence of adhesion on the thickness of the essential oil on the materials, the effect of adhesion on the transmittance of PS, the strength of adhesion point, and specificity of adhesion were determined. The optimum extraction conditions resulting in the extraction yield of 47.37 μL g were a ratio of liquid-to-solid of 8.94:1, a soaking time of 199.45 min, and an extraction time of 138.71 min. The major component in the essential oils was d-limonene (56.66%), followed by myrcene (6.62%). CrspEos presented a specific adherence effect on PS without influencing the thickness and transmittance of PS but with stronger tenacity than the parent material. CrspEos can be used as an environmentally friendly specific adhesive for PS.
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http://dx.doi.org/10.1021/acsomega.1c00895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173550PMC
June 2021

Construction and Application of EGCG-Loaded Lysozyme/Pectin Nanoparticles for Enhancing the Resistance of Nematodes to Heat and Oxidation Stresses.

Foods 2021 May 19;10(5). Epub 2021 May 19.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Novel nanoparticles (NPs) were constructed with lysozyme (LY) and pectin (Ps) through self-assembly, which were used as a carrier to encapsulate epigallocatechin-3-gallate (EGCG). The binding of EGCG and LY is a static quenching process. Hydrogen bonds might play a major role in the formation of NPs, which has also been verified by a lower binding constant of EGCG with LY/Ps NPs. Meanwhile, EGCG could lead to conformational and microenvironmental changes of LY, resulting in more folding of LY secondary structures. In addition, attaching Ps to LY might inhibit LY aggregation induced by addition of free EGCG. At the LY/Ps mass ratio of 1:1, the constructed LY/Ps NPs had a high EGCG-loading capacity without a significant change in mean particle size, thus, our NPs could be used as an effective nanocarrier for loading EGCG. In vivo, compared with free EGCG, EGCG loaded onto LY/Ps NPs significantly increased ' () resistance to heat stress and oxidative injury and prolonged their lifespan. This study provides theoretical basis and reference for constructing nanoactive substance carriers so as to improve the resistance of organisms to heat stress and oxidative damage and to increase their survival rate and extend their lifespan under environment stresses.
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http://dx.doi.org/10.3390/foods10051127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161057PMC
May 2021

DL-3-n-butylphthalide-induced neuroprotection in rat models of asphyxia-induced cardiac arrest followed by cardiopulmonary resuscitation.

J Cell Physiol 2021 Nov 1;236(11):7464-7472. Epub 2021 Jun 1.

Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Most patients that resuscitate successfully from cardiac arrest (CA) suffer from poor neurological prognosis. DL-3-n-butylphthalide (NBP) is known to have neuroprotective effects via multiple mechanisms. This study aimed to investigate whether NBP can decrease neurological impairment after CA. We studied the protective role of NBP in the hippocampus of a rat model of cardiac arrest induced by asphyxia. Thirty-nine rats were divided randomly into sham, control, and NBP groups. Rats in control and NBP groups underwent cardiopulmonary resuscitation (CPR) 6 min after asphyxia. NBP or vehicle (saline) was administered intravenously 10 min after the return of spontaneous circulation (ROSC). Ultrastructure of hippocampal neurons was observed under transmission electron microscope. NBP treatment improved neurological function up to 72 h after CA. The ultrastructural lesion in mitochondria recovered in the NBP-treated CA model. In conclusion, our study demonstrated multiple therapeutic benefits of NBP after CA.
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http://dx.doi.org/10.1002/jcp.30442DOI Listing
November 2021

Interactive effects of groundwater level and salinity on soil respiration in coastal wetlands of a Chinese delta.

Environ Pollut 2021 Oct 19;286:117400. Epub 2021 May 19.

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, China.

Coastal wetland soils serve as a great C sink or source, which highly depends on soil carbon flux affected by complex hydrology in relation to salinity. We conducted a field experiment to investigate soil respiration of three coastal wetlands with different land covers (BL: bare land; SS: Suaeda salsa; PL: Phragmites australis) from May to October in 2012 and 2013 under three groundwater tables (deeper, medium, and shallower water tables) in the Yellow River Delta of China, and to characterize the spatial and temporal changes and the primary environmental drivers of soil respiration in coastal wetlands. Our results showed that the elevated groundwater table decreased soil CO emissions, and the soil respiration rates at each groundwater table exhibited seasonal and diurnal dynamics, where significant differences were observed among coastal wetlands with different groundwater tables (p < 0.05), with the average CO emission of 146.52 ± 13.66 μmol ms for deeper water table wetlands, 105.09 ± 13.48 μmol ms for medium water table wetlands and 54.32 ± 10.02 μmol ms for shallower water table wetlands. Compared with bare land and Suaeda salsa wetlands, higher soil respiration was observed in Phragmites australis wetlands. Generally, soil respiration was greatly affected by salinity and soil water content. There were significant correlations between groundwater tables, electrical conductivity and soil respiration (p < 0.05), indicating that soil respiration in coastal wetlands was limited by electrical conductivity and groundwater tables and soil C sink might be improved by regulating water and salt conditions. We have also observed that soil respiration and temperature showed an exponential relationship on a seasonal scale. Taking into consideration the changes in groundwater tables and salinity that might be caused by sea level rise in the context of global warming, we emphasize the importance of groundwater level and salinity in the carbon cycle process of estuarine wetlands in the future.
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http://dx.doi.org/10.1016/j.envpol.2021.117400DOI Listing
October 2021

Kanglexin delays heart aging by promoting mitophagy.

Acta Pharmacol Sin 2021 May 25. Epub 2021 May 25.

Department of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, 150081, China.

Heart aging is characterized by structural and diastolic dysfunction of the heart. However, there is still no effective drug to prevent and treat the abnormal changes in cardiac function caused by aging. Here, we present the preventive effects of emodin and its derivative Kanglexin (KLX) against heart aging. We found that the diastolic dysfunction and cardiac remodeling in mice with D-galactose (D-gal)-induced aging were markedly mitigated by KLX and emodin. In addition, the senescence of neonatal mouse cardiomyocytes induced by D-gal was also reversed by KLX and emodin treatment. However, KLX exhibited better anti-heart aging effects than emodin at the same dose. Dysregulated mitophagy was observed in aging hearts and in senescent neonatal mouse cardiomyocytes, and KLX produced a greater increase in mitophagy than emodin. The mitophagy-promoting effects of KLX and emodin were ascribed to their abilities to enhance the protein stability of Parkin, a key modulator in mitophagy, with different potencies. Molecular docking and SPR analysis demonstrated that KLX has a higher affinity for the ubiquitin-like (UBL) domain of Parkin than emodin. The UBL domain might contribute to the stabilizing effects of KLX on Parkin. In conclusion, this study identifies KLX and emodin as effective anti-heart aging drugs that activate Parkin-mediated mitophagy and outlines their putative therapeutic importance.
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http://dx.doi.org/10.1038/s41401-021-00686-5DOI Listing
May 2021

Comparison of short-term outcomes and quality of life in totally laparoscopic distal gastrectomy and totally robotic distal gastrectomy for clinical stage I-III gastric cancer: study protocol for a multi-institutional randomised clinical trial.

BMJ Open 2021 05 25;11(5):e043535. Epub 2021 May 25.

Department of General Surgery & Institute of General Surgery, Chinese PLA General Hospital, Beijing, China

Introduction: Laparoscopic distal gastrectomy (LDG) is regarded as a standard treatment for patients with clinical stage I-III gastric cancer. With the popularisation of the robotic system in the 21st century, robotic distal gastrectomy has been increasingly applied, and its potential advantages over LDG have been proved by several studies. Intraperitoneal anastomosis is a hot topic in research as it highlights the superiority of minimally invasive surgery and is safe and feasible. We intend to conduct this randomised clinical trial to focus on short-term outcomes and quality of life (QOL) in totally laparoscopic distal gastrectomy (TLDG) and totally robotic distal gastrectomy (TRDG) for patients with clinical stage I-III gastric cancer.

Methods And Analysis: This study is a prospective, multi-institutional, open-label randomised clinical trial that will recruit 722 patients with a 1:1 ratio (361 patients in the TLDG group and 361 patients in the TRDG group) from eight large-scale gastrointestinal medical centres in China. The primary endpoint is 30-day postoperative morbidity. The secondary endpoints include QOL, 30-day severe postoperative morbidity and mortality, anastomotic-related complication rate, conversion to open surgery rate, intraoperative and postoperative indicators, operative and total costs during hospitalisation, 1-year overall survival and disease-free survival. QOL is determined by the The European Organization for Reasearch and Treatment of Cancer Quality of Life Questionnare-Core 30 and Stomach22 (EORTC QLQ-C30 and STO22) questionnaires which are completed before surgery and 1, 3, 6 months, and 1 year after surgery. χ test will be used for the primary endpoint, while analysis of covariance will be used to compare the overall changes of QOL between the two groups.

Ethics And Dissemination: This trial was approved by the Ethics Committee of the Chinese PLA General Hospital. The trial's results will be disseminated via peer-reviewed scientific journals and conference presentations.

Trial Registration Number: ChiCTR2000032670.
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http://dx.doi.org/10.1136/bmjopen-2020-043535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154927PMC
May 2021

Hydrological projections in the upper reaches of the Yangtze River Basin from 2020 to 2050.

Sci Rep 2021 05 6;11(1):9720. Epub 2021 May 6.

State Key Laboratory of Simulation and Regulation of Water Cycle in River Catchment, China Institute of Water Resources and Hydropower Research, Beijing, 100038, China.

Understanding the impact of climate change on runoff is essential for effective water resource management and planning. In this study, the regional climate model (RCM) RegCM4.5 was used to dynamically downscale near-future climate projections from two global climate models to a 50-km horizontal resolution over the upper reaches of the Yangtze River (UYRB). Based on the bias-corrected climate projection results, the impacts of climate change on mid-twenty-first century precipitation and temperature in the UYRB were assessed. Then, through the coupling of a large-scale hydrological model with RegCM4.5, the impacts of climate change on river flows at the outlets of the UYRB were assessed. According to the projections, the eastern UYRB will tend to be warm-dry in the near-future relative to the reference period, whereas the western UYRB will tend to be warm-humid. Precipitation will decreases at a rate of 19.05-19.25 mm/10 a, and the multiyear average annual precipitation will vary between - 0.5 and 0.5 mm/day. Temperature is projected to increases significantly at a rate of 0.38-0.52 °C/10 a, and the projected multiyear average air temperature increase is approximately 1.3-1.5 ℃. The contribution of snowmelt runoff to the annual runoff in the UYBR is only approximately 4%, whereas that to the spring runoff is approximately 9.2%. Affected by climate warming, the annual average snowmelt runoff in the basin will be reduced by 36-39%, whereas the total annual runoff will be reduced by 4.1-5%, and the extreme runoff will be slightly reduced. Areas of projected decreased runoff depth are mainly concentrated in the southeast region of the basin. The decrease in precipitation is driving this decrease in the southeast, whereas the decreased runoff depth in the northwest is mainly driven by the increase in evaporation.
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http://dx.doi.org/10.1038/s41598-021-88135-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102517PMC
May 2021
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