Publications by authors named "Hao Cheng"

775 Publications

Engineering Multifunctional Hydrogel With Osteogenic Capacity for Critical-Size Segmental Bone Defect Repair.

Front Bioeng Biotechnol 2022 9;10:899457. Epub 2022 May 9.

Department of Orthopaedic, Huizhou First Hospital, Guangdong Medical University, Huizhou, China.

Treating critical-size segmental bone defects is an arduous challenge in clinical work. Preparation of bone graft substitutes with notable osteoinductive properties is a feasible strategy for critical-size bone defects. Herein, a biocompatible hydrogel was designed by dynamic supramolecular assembly of polyvinyl alcohol (PVA), sodium tetraborate (NaBO), and tetraethyl orthosilicate (TEOS). The characteristics of the supramolecular hydrogel were evaluated by rheological analysis, swelling ratio, degradation experiments, and scanning electron microscopy (SEM). In experiments, this TEOS-hydrogel had self-healing property, low swelling rate, degradability, good biocompatibility, and induced osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by upregulating the expression of Runx-2, Col-1, OCN, and osteopontin (OPN). In segmental bone defect rabbit models, the TEOS-containing hydrogel accelerated bone regeneration, thus restoring the continuity of bone and recanalization of the medullary cavity. The abovementioned results demonstrated that this TEOS-hydrogel has the potential to realize bone healing in critical-size segmental bone defects.
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http://dx.doi.org/10.3389/fbioe.2022.899457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124794PMC
May 2022

lncR26319/miR-2834/EndophilinA axis regulates oogenesis of the silkworm, Bombyx mori.

Insect Sci 2022 May 25. Epub 2022 May 25.

Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan International Joint Laboratory of Insect Biology, College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, Henan Province, China.

Oocyte maturation is critical for insect reproduction. Vitellogenesis, the timely production and uptake of vitellogenin (Vg), is crucial for female fecundity. Vg is synthesized in fat body and absorbed by the oocytes through endocytosis during insect oogenesis. In the silkworm, Bombyx mori, we discovered that a nucleus-enriched long-noncoding RNA (lncRNA) lncR26319 regulates Endophilin A (EndoA) -a member of the endophilin family of endocytic proteins- through competitive bind to miR-2834. The lncR26319-miR-2834-EndoA axis was required for Vg endocytosis in the silkworm; loss of EndoA or overexpression of miR-2834 significantly reduced egg numbers in virgin moths. In addition, accumulation of miR-2834 resulted in pupal and adult deformation and reduced fecundity in females. The expression of Vg, 30-kDa (30K) protein and Egg-specific protein (Esp) decreased after knockdown of EndoA or overexpression of miR-2834, while knockdown of miR-2834 had an opposite effect on the expression of Vg, 30K protein gene and Esp. These results suggest that the lncR26319-miR-2834-EndoA axis contributes to the endocytic activity in the Vg uptake and leads to the normal progression of oogenesis in the silkworm. Thus, miR-2834 and EndoA are crucial for female reproduction and could be potential target for new pest management strategies in lepidopterans. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1744-7917.13082DOI Listing
May 2022

Electrochemiluminescence sensor based on Electrospun Crosslinked Carbon Nanofibers for the Detection of Difenidol Hydrochloride.

Comb Chem High Throughput Screen 2022 May 24. Epub 2022 May 24.

College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, P.R. China.

Background Cross-linked porous carbon nanofibers (CNF) was successfully prepared by electrospinning and high-temperature carbonization. Polyacrylonitrile (PAN) as the carbon source and Genipin as the cross-linking agent were used to prepare Cross-linked porous carbon nanofibers (CNF). Method and Material The field emission Scanning Electron Microscopy (SEM), Transmission Electron Microscope (TEM), Automatic Specific Surface and Porosity Analyzer Brunner Emmet Teller (BET), X-ray Diffraction (XRD), A laser confocal microspectroscope (Raman, XploRA PLUS, Horiba) were used to characterize materials. The CNF suspension was dropped on the surface of the bare glassy carbon electrode by the drip coating method to obtain a CNF modified electrode. Cyclic voltammetry was used to study the electrochemiluminescence behavior of difenidol hydrochloride on CNF modified glassy carbon electrode (Glassy Carbon Electrode, GCE). Results and Discussion Herein, we synthesised a kind of Crosslinked Carbon Nanofibers and designed a novel ECL biosensor, Under the optimal conditions, the concentration of difenidol hydrochloride has a linear relationship with the peak current in the range of 8.0×10-8 to 1.0×10-4mol/L, the correlation coefficient is R2=0.997, with a low detection limit (1.2×10- 8 mol/L). Difenidol hydrochloride in difenidol hydrochloride tablets was tested, and the recovery rate of sample addition was 83.17%-92.17%, and the RSD was <5.0%. The designed platform exhibited excellent analytical performance towards difenidol hydrochloride determination.
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http://dx.doi.org/10.2174/1386207325666220524093530DOI Listing
May 2022

eEF1A1 promotes colorectal cancer progression and predicts poor prognosis of patients.

Cancer Med 2022 May 24. Epub 2022 May 24.

State Key Laboratory of Cancer Biology and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

Colorectal cancer (CRC) is a major leading cause of cancer mortality worldwide in which dysregulated protein synthesis plays an etiologic role. The eukaryotic elongation factor 1 A1 (eEF1A1) exerts significant effects on protein synthesis by contributing to peptide chain extension. Whereas its role in CRC remains to be investigated. In this study, we found that the mRNA and protein levels of eEF1A1 were significantly upregulated in CRC cell lines and tissues. Elevated expression of eEF1A1 was correlated with shorter overall survival in 94 CRC patients. The inhibition of proliferation and cell cycle block were observed in CRC cells after eEF1A1 downregulation. Mechanistically, weighted gene correlation network analysis and further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that mitogen-activated protein kinases (MAPKs) signaling pathways were significantly enriched in high-eEF1A1 expression group, and the levels of phosphorylated p38/JNK/ERK MAPK were dramatically decreased after eEF1A1 downregulation. Overexpression of eEF1A1 in CRC correlated with a poor prognosis. Collectively, this study determined the oncogenic role of eEF1A1 in CRC proliferation and tumorigenesis. eEF1A1 might be a promising therapeutic target and prognostic biomarker in CRC.
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http://dx.doi.org/10.1002/cam4.4848DOI Listing
May 2022

A single-atom library for guided monometallic and concentration-complex multimetallic designs.

Nat Mater 2022 May 23. Epub 2022 May 23.

Department of Physics and Astronomy, University of California, Irvine, CA, USA.

Atomically dispersed single-atom catalysts have the potential to bridge heterogeneous and homogeneous catalysis. Dozens of single-atom catalysts have been developed, and they exhibit notable catalytic activity and selectivity that are not achievable on metal surfaces. Although promising, there is limited knowledge about the boundaries for the monometallic single-atom phase space, not to mention multimetallic phase spaces. Here, single-atom catalysts based on 37 monometallic elements are synthesized using a dissolution-and-carbonization method, characterized and analysed to build the largest reported library of single-atom catalysts. In conjunction with in situ studies, we uncover unified principles on the oxidation state, coordination number, bond length, coordination element and metal loading of single atoms to guide the design of single-atom catalysts with atomically dispersed atoms anchored on N-doped carbon. We utilize the library to open up complex multimetallic phase spaces for single-atom catalysts and demonstrate that there is no fundamental limit on using single-atom anchor sites as structural units to assemble concentration-complex single-atom catalyst materials with up to 12 different elements. Our work offers a single-atom library spanning from monometallic to concentration-complex multimetallic materials for the rational design of single-atom catalysts.
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http://dx.doi.org/10.1038/s41563-022-01252-yDOI Listing
May 2022

Predictors of refractory risk in systemic lupus erythematosus-related thrombocytopenia: a dual-centre retrospective study.

Lupus Sci Med 2022 May;9(1)

Department of Clinical Epidemiology, The First Hospital of Jilin University, Changchun, China

Objectives: Based on clinical and laboratory indicators, this study aimed to establish a multiparametric nomogram to assess the risk of refractory cases of SLE-related thrombocytopenia (SLE-related TP) before systematic treatment.

Methods: From June 2012 to July 2021, a dual-centre retrospective cohort study of prospectively collected data of patients with SLE-related TP was conducted. The cohort data were divided into a developing set, internal validation set and external validation set. Refractory thrombocytopenia (RTP) was defined as failed to prednisone at 1 mg/kg per day with a platelet count cannot achieve or maintain higher than 50×10/L. In the developing set, a nomogram were established to predict RTP risk based on clinical characteristics and laboratory indicators by multivariable logistic regression, and its performance was assessed by receiver operating characteristic (ROC) curves, calibration curves, decision curve analysis (DCA) and clinical impact curve (CIC).

Results: A total of 1778 patients with SLE were included, and 413 eligible patients were involved in the final analysis with 121 RTPs. The RTP risk assessment (RRA) model was composed of five significant risk variables: pregnancy, severity of TP, complement 3, anticardiolipin antibody-immunoglobulin G and autoimmune haemolytic anaemia. In three datasets, the AUCs were 0.887 (95% CI 0.830 to 0.945), 0.880 (95% CI 0.785 to 0.975) and 0.871 (95% CI 0.793 to 0.949), respectively. The calibration curve, DCA and CIC all showed good performance of the RRA model.

Conclusion: The RRA model demonstrated good capability for assessing the refractory risk in SLE-related TP, which may be helpful for early identification and intervention.
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http://dx.doi.org/10.1136/lupus-2022-000677DOI Listing
May 2022

PD-1 Mediates Decidual γδ T Cells Cytotoxicity During Recurrent Pregnancy Loss.

Am J Reprod Immunol 2022 May 14. Epub 2022 May 14.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Problem: Recurrent pregnancy loss (RPL) is one of the big challenges of normal pregnancy. Immune dysregulation has been proposed for the key underline mechanisms of RPL. However, the essential roles of T cells, especially γδ T cells, have not been defined.

Method Of Study: Decidua were obtained from normal pregnancy women or recurrent pregnancy loss patients and the surface molecules of γδ T cells in decidua were evaluated via flow cytometric analysis. The expression of PD-1 in clinical samples was analyzed by immunohistochemistry assay. The intracellular cytokines of decidual PD-1+ and PD-1- γδ T cells were evaluated by flow cytometric analysis. The cytotoxicity of PD-1- γδ T cells were confirmed via an in vitro co-culture experiment. The specific inhibitors for Erk, p38 and JNK against the MAPK pathway were added to the co-culture media to evaluate the functions of the Erk, p38 and JNK.

Results: We demonstrated that PD-1 was significantly decreased on decidual tissue γδ T cells of patients with RPL, resulting in the enhanced cytotoxicity of γδ T cells against trophoblasts. We further elucidated an Erk-dependent TNF-α production mediates the γδ T cell cytotoxicity against the trophoblast cells. Finally, the reduced expression of PD-L1 in the villi tissues of patients with RPL might be the cause of the reduction of PD-1 on the tissue γδ T cells.

Conclusion: Our study uncovers an important role of PD-1 expression on decidual γδ T cells in maintaining the normal pregnancy, and may provide a new strategy for immune therapy against RPL. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/aji.13562DOI Listing
May 2022

Sensitive and selective electrochemical determination of uric acid in urine based on ultrasmall iron oxide nanoparticles decorated urchin-like nitrogen-doped carbon.

Colloids Surf B Biointerfaces 2022 May 5;216:112538. Epub 2022 May 5.

Key Laboratory of Anti-inflammatory and Immune Medicines, Ministry of Education, School of Pharmacy, Anhui Medical University, Hefei 230032, PR China. Electronic address:

Hypercrosslinked pyrrole was synthesized via the Friedel-Crafts reaction and then carbonized to obtain urchin-like nitrogen-doped carbon (UNC). Ultrasmall iron oxide nanoparticles were then supported on UNC, and the composite was used to prepare an electrochemical sensor for detecting uric acid (UA) in human urine. FeO/UNC was characterized and analyzed via scanning electron microscopy, transmission electron microscopy, energy dispersive spectrometry, X-ray diffraction, and X-ray photoelectron spectroscopy. A glassy carbon electrode (GCE) modified with FeO/UNC was used as an electrochemical sensor to effectively identify UA. The electrochemical behavior of the FeO/UNC-based UA sensor was studied using differential pulse stripping voltammetry, and the optimal conditions were determined by changing the amount of FeO/UNC, pH of the buffer solution, deposition potential, and deposition time. Under optimal conditions, the FeO/UNC-based electrochemical sensor detected UA in the range of 2-200 μM, where the limit of detection (LOD) for UA was 0.29 μM. Anti-interference experiments were performed, and the sensor was applied to the actual analysis of human urine samples. Urea, glucose, ascorbic acid, and many cations and anions present at 100-fold concentrations relative to UA did not strongly interfere with the response of the sensor to UA. The FeO/UNC electrochemical sensor has high sensitivity and selectivity for uric acid in human urine samples and can be used for actual clinical testing of UA in urine.
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http://dx.doi.org/10.1016/j.colsurfb.2022.112538DOI Listing
May 2022

[Giant nodule and keratinized facial basal cell carcinoma: a case report].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2022 Apr;36(4):307-309

A case of large ulcerative basal cell carcinoma was reported here. The patient had a history of skin ulceration on the right face 20 years ago, which has not been paid attention to. Recently the area of ulcer gradually increasing, and consciously affects the function, so the patient came to our hospital for the sake of treatment. The diagnosis of right facial basal cell carcinoma (T4NXMX) was made by MRI, CT and histopathological examination. After improving the preoperative examination, surgical treatment was performed on May 10, 2020, the postoperative flap survived, the face recovered well, there was no recurrence after 6 months follow-up, the result was satisfied, and it is still in the follow-up.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2022.04.015DOI Listing
April 2022

Construction of an Immune-Related lncRNA Signature That Predicts Prognosis and Immune Microenvironment in Osteosarcoma Patients.

Front Oncol 2022 14;12:769202. Epub 2022 Apr 14.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Osteosarcoma is one of the most common bone tumors in teenagers. We hope to provide a reliable method to predict the prognosis of osteosarcoma and find potential targets for early diagnosis and precise treatment. To address this issue, we performed a detailed bioinformatics analysis based on the Cancer Genome Atlas (TCGA). A total of 85 osteosarcoma patients with gene expression data and clinicopathological features were included in this study, which was considered the entire set. They were randomly divided into a train set and a test set. We identified six lncRNAs (ELFN1-AS1, LINC00837, OLMALINC, AL669970.3, AC005332.4 and AC023157.3), and constructed a signature that exhibited good predictive ability of patient survival and metastasis. What's more, we found that risk score calculated by the signature was positively correlated to tumor purity, CD4 naive T cells, and negatively correlated to CD8 T cells. Furthermore, we investigated each lncRNA in the signature and found that these six lncRNAs were associated with tumorigenesis and immune cells in the tumor microenvironment. In conclusion, we constructed and validated a signature, which had good performance in the prediction of survival, metastasis and immune microenvironment. Our study indicated possible mechanisms of these lncRNAs in the development of osteosarcoma, which may provide new insights into the precise treatment of osteosarcoma.
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http://dx.doi.org/10.3389/fonc.2022.769202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047752PMC
April 2022

VTag: a Semi-Supervised Pipeline for Tracking Pig Activity with a Single Top-View Camera.

J Anim Sci 2022 Apr 29. Epub 2022 Apr 29.

Department of Animal Science, University of California, Davis, CA, USA.

Precision livestock farming has become an important research focus with the rising demand of meat production in the swine industry. Currently, the farming practice is widely conducted by the technology of computer vision (CV), which automates monitoring pig activity solely based on video recordings. Automation is fulfilled by deriving imagery features that can guide CV systems to recognize animals' body contours, positions, and behavioral categories. Nevertheless, the performance of the CV systems is sensitive to the quality of imagery features. When the CV system is deployed in a variable environment, its performance may decrease as the features are not generalized enough under different illumination conditions. Moreover, most CV systems are established by supervised learning, in which intensive effort in labeling ground truths for the training process is required. Hence, a semi-supervised pipeline, VTag, is developed in this study. The pipeline focuses on long-term tracking of pig activity without requesting any pre-labeled video but a few human supervisions to build a CV system. The pipeline can be rapidly deployed as only one top-view RGB camera is needed for the tracking task. Additionally, the pipeline was released as a software tool with a friendly graphical interface available to general users. Among the presented datasets, the average tracking error was 17.99 centimeters. Besides, with the prediction results, the pig moving distance per unit time can be estimated for activity studies. Lastly, as the motion is monitored, a heat map showing spatial hot spots visited by the pigs can be useful guidance for farming management. The presented pipeline saves massive laborious work in preparing training dataset. The rapid deployment of the tracking system paves the way for pig behavior monitoring.
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http://dx.doi.org/10.1093/jas/skac147DOI Listing
April 2022

Objective Phenotyping of Root System Architecture Using Image Augmentation and Machine Learning in Alfalfa (Medicago sativa L.).

Plant Phenomics 2022 7;2022:9879610. Epub 2022 Apr 7.

USDA-ARS, Plant Science Research Unit, 1991 Upper Buford Circle, St. Paul, MN 55108, USA.

Active breeding programs specifically for root system architecture (RSA) phenotypes remain rare; however, breeding for branch and taproot types in the perennial crop alfalfa is ongoing. Phenotyping in this and other crops for active RSA breeding has mostly used visual scoring of specific traits or subjective classification into different root types. While image-based methods have been developed, translation to applied breeding is limited. This research is aimed at developing and comparing image-based RSA phenotyping methods using machine and deep learning algorithms for objective classification of 617 root images from mature alfalfa plants collected from the field to support the ongoing breeding efforts. Our results show that unsupervised machine learning tends to incorrectly classify roots into a normal distribution with most lines predicted as the intermediate root type. Encouragingly, random forest and TensorFlow-based neural networks can classify the root types into branch-type, taproot-type, and an intermediate taproot-branch type with 86% accuracy. With image augmentation, the prediction accuracy was improved to 97%. Coupling the predicted root type with its prediction probability will give breeders a confidence level for better decisions to advance the best and exclude the worst lines from their breeding program. This machine and deep learning approach enables accurate classification of the RSA phenotypes for genomic breeding of climate-resilient alfalfa.
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http://dx.doi.org/10.34133/2022/9879610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9012978PMC
April 2022

Molecular Design of Two-Dimensional Covalent Heptazine Frameworks for Photocatalytic Overall Water Splitting under Visible Light.

J Phys Chem Lett 2022 May 27;13(17):3949-3956. Epub 2022 Apr 27.

School of Chemistry and Materials Sciences, CAS Key Lab of Materials for Energy Conversion, Synergetic Innovation of Quantum Information & Quantum Technology, and CAS Center for Excellence in Nanoscience, University of Science and Technology of China, Hefei, Anhui 230026, China.

Photocatalytic water splitting sustainably offers clean hydrogen energy, but it is challenging to produce low-cost photocatalysts that split water stoichiometrically into H and O without sacrificial agents under visible light. Here, we designed 17 two-dimensional (2D) covalent heptazine frameworks (CHFs) by topologically assembling heptazine and benzene-containing molecular units that provide active sites for hydrogen and oxygen evolution reactions, respectively. Among them, 12 CHFs have band gap values of <3.0 eV with band margins straddling the chemical reaction potential of H/H and O/HO. In particular, a 2D [email protected] CHF based on heptazine and 4,7-diphenyl-2,1,3-benzothiadiazole is a potential photocatalyst with a band gap of 2.47 eV for overall water splitting, which was confirmed with the calculated Gibbs free energy, non-adiabatic molecular dynamics, and preliminary experiment. This study presents an experimentally feasible molecular design of 2D CHFs as metal-free photocatalysts for overall water splitting under visible light.
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http://dx.doi.org/10.1021/acs.jpclett.2c00819DOI Listing
May 2022

Arbitrary active control of the Pancharatnam-Berry phase in a terahertz metasurface.

Opt Express 2022 Mar;30(7):11444-11458

Active phase-control metasurfaces show outstanding capability in the active manipulation of light propagation, while the previous active phase control methods have many constraints in the cost of simulation or the phase modulation range. In this paper, we design and demonstrate a phase controlled metastructure based on two circular split ring resonators (CSRRs) composed of silicon and Au with different widths, which can continuously achieve an arbitrary Pancharatnam-Berry (PB) phase between -π and π before or after active control. The PB phase of such a metasurface before active control is determined by the rotation angle of the Au-composed CSRR, while the PB phase after active control is determined by the rotation angle of the silicon-composed CSRR. And active control of the PB phase is realized by varying conductivity of silicon under an external optical pump. Based on this metastructure, active control of light deflection, metalens with arbitrary reconfigurable focal points and achromatic metalens under selective frequencies are designed and simulated. Moreover, the experimental results demonstrate that focal spots of metalens can be actively controlled by the optical pump, in accord with the simulated ones. Our metastructure implements a plethora of metasurfaces' active phase modulation and provides applications in active light manipulation.
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http://dx.doi.org/10.1364/OE.450117DOI Listing
March 2022

4.8% sevoflurane induces activation of autophagy in human neuroblastoma SH-SY5Y cells by the AMPK/mTOR signaling pathway.

Neurotoxicology 2022 May 23;90:256-264. Epub 2022 Apr 23.

Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei 230022, Anhui, PR China. Electronic address:

Prolonged sevoflurane exposure leads to neurotoxicity. Autophagy plays an important role in promoting cell survival in different conditions. However, the role and mechanism of autophagy in sevoflurane-induced neurotoxicity were not fully elucidated. We attempted to indicate whether sevoflurane could activate the AMP-activated protein kinase (AMPK)/mechanistic target of rapamycin (mTOR)-mediated autophagy to attenuate anesthetics-induced neuronal injury in this study. Sevoflurane treatment significantly decreased the cell viability and induced apoptosis of SH-SY5Y cells. The expression level of Bcl-2 decreased, while that of Bax remarkably increased. Meanwhile, autophagy was activated by sevoflurane exposure as evidenced by increased expression levels of autophagy-related proteins (LC3-II and Atg5), decreased expression level of autophagic substrate P62, and increased autophagosomes and autolysosomes. Further autophagosomes and fewer autolysosomes were observed in the presence of Bafilomycin A1, an autolysosomes degradation inhibitor, suggesting that sevoflurane induced autophagic flux rather than inhibiting degradation of autophagy. Activation of autophagy by rapamycin partly reversed the sevoflurane-decreased cell viability. In contrast, inhibition of autophagy by 3-Methyladenine (3-MA) or Atg5-targeted small interfering RNA (siRNA) aggravated the sevoflurane-induced neurotoxicity. Further examination revealed that sevoflurane-induced autophagy was mediated by the AMPK/mTOR signaling pathway, with increased p-AMPK expression and decreased p-mTOR expression. Collectively, these results indicated that sevoflurane activates autophagy by regulating the AMPK/mTOR signaling pathway, which is protective against sevoflurane-induced damage in SH-SY5Y cells. Our results may assist clinicians to develop further promising therapeutic strategies for the neurotoxicity induced by inhaled anesthetics.
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http://dx.doi.org/10.1016/j.neuro.2022.04.008DOI Listing
May 2022

Editorial: Multi-Layered Genome-Wide Association/Prediction in Animals.

Front Genet 2022 8;13:877748. Epub 2022 Apr 8.

College of Animal Science, South China Agricultural University, Guangzhou, China.

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http://dx.doi.org/10.3389/fgene.2022.877748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023786PMC
April 2022

butyricum RH2 Alleviates Chronic Foot Shock Stress-Induced Behavioral Deficits in Rats via PAI-1.

Front Pharmacol 2022 6;13:845221. Epub 2022 Apr 6.

Jiangsu Vocational College of Medicine, Yancheng, China.

Recent investigations have demonstrated that the chronic stress-induced behavioral disorders can be ameliorated by probiotics including (C. butyricum) via the gut-brain-axis. However, the molecular mechanisms underlying the beneficial effects of C. butyricum on brain remain largely unknown. Here, we investigated whether chronic foot shock stress (CFSS) paradigm used for a hypertensive animal model could induce mood disorders such as anxiety, depression and cognitive impairments. Then, we assessed the impact of RH2 on the behavior disorders and neurobiological alterations in the hippocampus. Male Sprague-Dawley (SD) rats received intermittent electric shocks for consecutive 14 days and were treated with RH2 for 17 days. Anxiety- or depression-like behaviors were evaluated by open field test (OFT), and elevated plus maze (EPM). The Morris water maze test (MWM) was used to evaluate the cognitive functions. CFSS intervention led to mild anxiety- or depression-like behavior or cognitive impairment and RH2 treatment reversed the CFSS-induced symptoms. The serum ACTH or CORT was increased following CFSS but was completely reversed by RH2 treatment. In the hippocampus of CFSS rats, the expressions of BDNF and TrkB were downregulated but proBDNF and P75 were upregulated. These expression changes were partially reversed by RH2, suggesting a mode of action on BDNF and proBDNF balance. CFSS exposure resulted in downregulation of tissue-type plasminogen activator (tPA) but upregulation of plasminogen activator inhibitor 1(PAI-1), which could contribute to the decrease in BDNF by reduced conversion from proBDNF to BDNF in the hippocampus. RH2 treatment reversed the upregulated PAI-1 but not the downregulated tPA, which was in parallel with the amelioration of behavioral abnormalities, suggesting a novel tPA independent mechanism for PAI-1 action. Our results demonstrate for the first time that RH2 attenuates stress-induced behavior disorders via inhibiting the expression of brain PAI-1.
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http://dx.doi.org/10.3389/fphar.2022.845221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019525PMC
April 2022

Resveratrol Stabilization and Loss by Sodium Caseinate, Whey and Soy Protein Isolates: Loading, Antioxidant Activity, Oxidability.

Antioxidants (Basel) 2022 Mar 28;11(4). Epub 2022 Mar 28.

State Key Lab of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

The interaction of protein carrier and polyphenol is variable due to their environmental sensitivity. In this study, the interaction between resveratrol and whey protein isolate (WPI), sodium caseinate (SC) and soy protein isolate (SPI) during storage were systematically investigated from the aspects of polyphenol loading, antioxidant activity and oxidability. It was revealed that resveratrol loaded more in the SPI core and existed both in the core of SC micelles and on the particle surface, while WPI and resveratrol mainly formed in complexes. The loading capacity of the three proteins ranked in order SC > SPI > WPI. ABTS assay showed that the antioxidant activity of the protein carriers in the initial state was SC > SPI > WPI. The results of sulfhydryl, carbonyl and amino acid analysis showed that protein oxidability was SPI > SC > WPI. WPI, with the least oxidation, improved the storage stability of resveratrol, and the impact of SC on resveratrol stability changed from a protective to a pro-degradation effect. Co-oxidation occurred between SPI and resveratrol during storage, which refers to covalent interactions. The data gathered here suggested that the transition between the antioxidant and pro-oxidative properties of the carrier is the primary factor to investigate its protective effect on the delivered polyphenol.
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http://dx.doi.org/10.3390/antiox11040647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030250PMC
March 2022

Genome-wide association analysis discovered new loci and candidate genes associated with low-phosphorus tolerance based on shoot mineral elements concentrations in soybean.

Mol Genet Genomics 2022 May 20;297(3):843-858. Epub 2022 Apr 20.

National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China.

Phosphorus (P) deficiency is one of the major limitations for soybean production. Moreover, it has been well reported P and other mineral elements function interdependently or antagonistically to control nutrients homeostasis in plants. Thus, it is urgently needed to understand the genetic mechanism of the accumulation of mineral elements in response to low-P stress. In this study, to identify single nucleotide polymorphisms (SNPs) and candidate genes controlling the accumulation of mineral elements suffering low-P stress in seedling stage of soybean plants, we measured concentrations of mineral elements, including P, Zn, Fe, Mn, Mg and Ca, in shoots of 211 soybean accessions under normal phosphorus (+P) and low phosphorus (-P) conditions in two hydroponic experiments. And genome-wide association study (GWAS) using high density NJAU 355K SoySNP array and concentrations of five of these mineral elements except P was performed. A total of 36 SNPs distributed on 13 chromosomes were identified to be significantly associated with low-P tolerance, and nine SNPs on chromosome 10 formed a SNP cluster. Meanwhile, the candidate gene GmFeB1 was found to serve as a negative regulator element involved in soybean P metabolism and the haplotype1 (Hap1) of GmFeB1 showed significantly higher shoot Fe concentration under -P condition than that of Hap2. In summary, we uncover 36 SNPs significantly associated with shoot mineral elements concentrations under different P conditions and a soybean low-P related gene GmFeB1, which will provide additional genetic information for soybean low-P tolerance and new gene resources for P-efficient soybean varieties breeding.
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http://dx.doi.org/10.1007/s00438-022-01895-1DOI Listing
May 2022

Nerve transfer with 3D-printed branch nerve conduits.

Burns Trauma 2022 15;10:tkac010. Epub 2022 Apr 15.

Department of Neurosurgery, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.

Background: Nerve transfer is an important clinical surgical procedure for nerve repair by the coaptation of a healthy donor nerve to an injured nerve. Usually, nerve transfer is performed in an end-to-end manner, which will lead to functional loss of the donor nerve. In this study, we aimed to evaluate the efficacy of 3D-printed branch nerve conduits in nerve transfer.

Methods: Customized branch conduits were constructed using gelatine-methacryloyl by 3D printing. The nerve conduits were characterized both and . The efficacy of 3D-printed branch nerve conduits in nerve transfer was evaluated in rats through electrophysiology testing and histological evaluation.

Results: The results obtained showed that a single nerve stump could form a complex nerve network in the 3D-printed multibranch conduit. A two-branch conduit was 3D printed for transferring the tibial nerve to the peroneal nerve in rats. In this process, the two branches were connected to the distal tibial nerve and peroneal nerve. It was found that the two nerves were successfully repaired with functional recovery.

Conclusions: It is implied that the two-branch conduit could not only repair the peroneal nerve but also preserve partial function of the donor tibial nerve. This work demonstrated that 3D-printed branch nerve conduits provide a potential method for nerve transfer.
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http://dx.doi.org/10.1093/burnst/tkac010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9012979PMC
April 2022

Polarization Maintaining Fiber Temperature and Stress Gradient Sensitization Sensor Based on Semiconductor-Metal-Polymer Three-Layer Film Coating.

ACS Appl Mater Interfaces 2022 May 19;14(17):20053-20061. Epub 2022 Apr 19.

College of Physics and Optoelectronics, Faculty of Science, Beijing University of Technology, Beijing 100124, China.

Increasing sensitivity, measuring points, and stability have always been the pursuit of sensors. ZnSe:CO and Ag composite nano films were coated on polarization maintaining fiber (PMF). Then, the coated PMF was nested in capillary and hose which was encapsulated with polydimethylsiloxane (PDMS) and epoxy resin. The integrated capillary sensor and thermoplastic hose sensor were prepared. The gradient sensitization of various measurement parameters such as temperature, stress, and micro bending is realized. The temperature sensitivity is 1.49 nm/°C, the micro bending sensitivity is 1.72 nm/10 g, and the stress sensitivity is 6.27 nm/mε. The sensors maintain good linearity and instantaneous response while having high sensitivity. By adjusting the length of PMF, the number of troughs is increased in the same band range, and different troughs have different sensitivities, which solves the inherent problem of cross sensitivity and realizes multiparameter measurement. Capillary sensors are used for remote safe real-time monitoring of mechanical overheating, and hose sensors are used for real-time monitoring of bridge load and human joint bending. This work is of great significance to the extension of the application range of optical fiber sensor.
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http://dx.doi.org/10.1021/acsami.2c03102DOI Listing
May 2022

Disruption of peroxisome proliferator-activated receptor α in hepatocytes protects against acetaminophen-induced liver injury by activating the IL-6/STAT3 pathway.

Int J Biol Sci 2022 6;18(6):2317-2328. Epub 2022 Mar 6.

Department of Infectious Diseases, Peking University First Hospital, Beijing, 100034, China.

Peroxisome proliferator-activated receptor α (PPARα) is a ligand-activated transcription factor abundantly expressed in liver. PPARα activator has been previously reported to protect against acetaminophen-induced hepatotoxicity, but fenofibrate, a lipid-lowering drug that activates PPARα, has a common side-effect causing liver injury. Thus, the exact effect of liver PPARα on drug-induced liver injury remains obscure. Hepatocyte-specific knockout mice and littermate wild-type control mice were intraperitoneally injected with acetaminophen (400 mg/kg body weight). Blood and liver samples were collected at different time points. We measured phase I and II cytochrome P450 enzymes, glutathione, reactive oxygen species, cytokines including , and pSTAT3 by reverse transcriptase quantitative PCR, colorimetric, immunohistochemistry analyses and Western blotting. Hepatic expression of PPARα was significantly decreased in DILI patients. Disruption of the gene in hepatocytes significantly reduced acetaminophen-induced liver injury in mice. ROS production rather than the expression levels of phase I and II cytochrome P450 enzymes was reduced in hepatocyte-specific knockout mice compared to control mice after acetaminophen administration. Mechanistically, hepatocyte-specific knockout mice had upregulated activation of the hepatoprotective pathway IL-6/STAT3 compared to wild-type mice, as evidenced by hepatic mRNA levels, hepatic protein levels of STAT3 and phosphorylated STAT3 were much higher in hepatocyte-specific knockout mice than in wild-type mice post acetaminophen injection. Hepatocyte-specific disruption of the gene protects against acetaminophen-induced liver injury by reducing oxidative stress and upregulating the hepatoprotective IL-6/STAT3 signaling pathway.
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http://dx.doi.org/10.7150/ijbs.69609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990472PMC
April 2022

High fructose diet: A risk factor for immune system dysregulation.

Hum Immunol 2022 Jun 9;83(6):538-546. Epub 2022 Apr 9.

Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address:

Excessive intake of sweets is a predisposing factor for metabolic disorders, and fructose, as one of the major dietary sugars in the diet, has been shown to be a major cause of obesity, diabetes, and metabolic syndrome. These disorders are usually associated with immune dysfunction. Therefore, exploring the effects of a high fructose diet on the immune system may provide insight into the underlying mechanisms of these diseases. We synthesized the available evidence to suggest that excessive fructose intake disrupts the body's immune homeostasis by promoting immune cell metabolic rearrangements, alterations in gut microbial community structure, and intestinal barrier permeability. Indeed, not only does fructose itself affect immune system homeostasis, but its metabolites also have a profound influence. The metabolites from fructolysis are mainly produced in the small intestine and liver and subsequently enter the systemic circulation. Elevated levels of fructose metabolites, such as uric acid, FFAs, and lactate, are closely associated with oxidative stress and local tissue and organ inflammatory responses. In this review, we will focus on the link between fructose and inflammatory responses. In the meanwhile, we will also briefly summarize the studies of cancer development and immune escape mediated by fructose, as it might be beneficial for cancer immunotherapy.
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http://dx.doi.org/10.1016/j.humimm.2022.03.007DOI Listing
June 2022

Integration of Light and Auxin Signaling in Shade Plants: From Mechanisms to Opportunities in Urban Agriculture.

Int J Mol Sci 2022 Mar 22;23(7). Epub 2022 Mar 22.

Laboratory of Space Biology, Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610213, China.

With intensification of urbanization throughout the world, food security is being threatened by the population surge, frequent occurrence of extreme climate events, limited area of available cultivated land, insufficient utilization of urban space, and other factors. Determining the means by which high-yielding and high-quality crops can be produced in a limited space is an urgent priority for plant scientists. Dense planting, vertical production, and indoor cultivation are effective ways to make full use of space and improve the crop yield. The results of physiological and molecular analyses of the model plant species have shown that the plant response to shade is the key to regulating the plant response to changes in light intensity and quality by integrating light and auxin signals. In this study, we have summarized the major molecular mechanisms of shade avoidance and shade tolerance in plants. In addition, the biotechnological strategies of enhancing plant shade tolerance are discussed. More importantly, cultivating crop varieties with strong shade tolerance could provide effective strategies for dense planting, vertical production, and indoor cultivation in urban agriculture in the future.
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http://dx.doi.org/10.3390/ijms23073422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998421PMC
March 2022

A Non-Volatile Tunable Ultra-Compact Silicon Photonic Logic Gate.

Nanomaterials (Basel) 2022 Mar 28;12(7). Epub 2022 Mar 28.

Center of Material Science, National University of Defense Technology, Changsha 410073, China.

Logic gates, as one of the most important basic units in electronic integrated circuits (EICs), are also equally important in photonic integrated circuits (PICs). In this study, we proposed a non-volatile, ultra-compact all-photonics logic gate. The footprint is only 2 μm × 2 μm. We regulate the phase change of optical phase change materials(O-PCMs) SbSe to switch the function of the logic gate. The SbSe possess a unique non-volatile optical phase change function; therefore, when SbSe is in the crystalline or amorphous state, our device can work as XOR gate or AND gate, and our designed logic '1' and logic '0' contrasts reach 11.8 dB and 5.7 dB at 1550 nm, respectively. Compared with other traditional optical logic gates, our device simultaneously has non-volatile characteristics, tunability, and additionally an ultra-small size. These results could fully meet the needs of fusion between PICs and EICs, and developing truly chip-scale optoelectronic logic solution.
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http://dx.doi.org/10.3390/nano12071121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000527PMC
March 2022

Genome-wide association studies for egg quality traits in White Leghorn layers using low-pass sequencing and SNP chip data.

J Anim Breed Genet 2022 Apr 11. Epub 2022 Apr 11.

Hy-Line International, Dallas Center, Iowa, USA.

Low-pass sequencing data have been proposed as an alternative to single nucleotide polymorphism (SNP) chips in genome-wide association studies (GWAS) of several species. However, it has not been used in layer chickens yet. This study aims at comparing the GWAS results of White Leghorn chickens using low-pass sequencing data (1×) and 54 k SNP chip data. Ten commercially relevant egg quality traits including albumen height, shell strength, shell colour, egg weight and yolk weight collected from up to 1,420 White Leghorn chickens were analysed. The results showed that the genomic heritability estimates based on low-pass sequencing data were higher than those based on SNP chip data. Although two GWAS analyses showed similar overall landscape for most traits, low-pass sequencing captured some significant SNPs that were not on the SNP chip. In GWAS analysis using 54 k SNP chip data, after including more individuals (up to 5,700), additional significant SNPs not detected by low-pass sequencing data were found. In conclusion, GWAS using low-pass sequencing data showed similar results to those with SNP chip data and may require much larger sample sizes to show measurable advantages.
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http://dx.doi.org/10.1111/jbg.12679DOI Listing
April 2022

Downregulation of a gibberellin 3β-hydroxylase enhances photosynthesis and increases seed yield in soybean.

New Phytol 2022 Apr 9. Epub 2022 Apr 9.

National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China.

Seed yield, determined mainly by seed numbers and seed weight, is the primary target of soybean breeding. Identifying the genes underlying yield-related traits is of great significance. Through joint linkage mapping and a genome-wide association study for 100-seed weight, we cloned GmGA3ox1, a gene encoding gibberellin 3β-hydroxylase, which is the key enzyme in the gibberellin synthesis pathway. Genome resequencing identified a beneficial GmGA3ox1 haplotype contributing to high seed weight, which was further confirmed by soybean transformants. CRISPR/Cas9-generated gmga3ox1 mutants showed lower seed weight, but promoted seed yield by increasing seed numbers. The gmga3ox1 mutants reduced gibberellin biosynthesis while enhancing photosynthesis. Knockout of GmGA3ox1 resulted in the upregulation of numerous photosynthesis-related genes, particularly the GmRCA family encoding ribulose-1,5-bispho-sphate carboxylase-oxygenase (Rubisco) activases. The basic leucine zipper transcription factors GmbZIP97 and GmbZIP159, which were both upregulated in the gmga3ox1 mutants and induced by the gibberellin synthesis inhibitor uniconazole, could bind to the promoter of GmRCAβ and activate its expression. Analysis of genomic sequences with over 2700 soybean accessions suggested that GmGA3ox1 is being gradually utilized in modern breeding. Our results elucidated the important role of GmGA3ox1 in soybean yield. These findings reveal important clues for future high-yield breeding in soybean and other crops.
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http://dx.doi.org/10.1111/nph.18153DOI Listing
April 2022

Quantitative assessment of the aging corpus cavernosum by shear wave elastography.

Asian J Androl 2022 Mar 29. Epub 2022 Mar 29.

Department of Urology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

We wanted to determine whether shear wave elastography (SWE) could be used to evaluate the aging degree of the corpus cavernosum (CC) and to identify the histological basis of changes in SWE measurements during the aging process. We performed a cross-sectional study enrolling healthy participants of different ages. We measured the Young's modulus (YM) of the penile CCs by SWE and assessed erectile function using the International Index of Erectile Function-5 (IIEF-5). Histological investigation was performed in surgically resected penile specimens from a separate group of patients to examine the smooth muscle and collagen content of the CCs. Furthermore, we measured the YM, erectile function, smooth muscle, and collagen content of the CCs in different age groups of rats. Finally, we enrolled 210 male volunteers in this study. The YM of the CC (CCYM) was positively correlated with age (r = 0.949, P < 0.01) and negatively correlated with erectile function (r = -0.843, P < 0.01). Histological examinations showed that CCs had increased collagen content but decreased smooth muscle content with increased age. The same positive correlation between CCYM and age was also observed in the animal study. In addition, the animal study showed that older rats, with increased CCYM and decreased erectile function, had lower smooth muscle content and higher collagen content. SWE can noninvasively and quantitatively evaluate the aging degree of the CC. Increased collagen content and decreased smooth muscle content might be the histological basis for the effect of aging on the CC and the increase in its YM.
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http://dx.doi.org/10.4103/aja2021119DOI Listing
March 2022

Human Papillomavirus Type 16 Early Protein E7 Activates Autophagy through Inhibition of Dual-Specificity Phosphatase 5.

Oxid Med Cell Longev 2022 10;2022:1863098. Epub 2022 Mar 10.

Department of Dermatology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Consistent high-risk human papillomavirus (HPV) infection leads to various malignant cancers. Autophagy can promote cancer progression by helping cancer cells survive under stress or induce oncogenic effects when mutations or abnormalities occur. Mitogen activated protein kinases (MAPKs) can transduce various external or intrinsic stimuli into cellular responses, including autophagy, and dual-specificity phosphates (DUSPs) contribute to the direct regulation of MAPK activities. Previously, we showed that expression of DUSP5 was repressed in HPV16 E7-expressing normal human epidermal keratinocytes (NHEKs). Here we show that clinical HPV16 E7-positive precancerous and cancerous tissues also demonstrate low DUSP5 levels compared with control tissues, indicating that the inverse correlation between HPV16 E7 and DUSP5 is clinically relevant. We furthermore investigated the autophagy response in both DUSP5-deficient and HPV16 E7-expressing NHEKs. Confocal microscopy and Western analysis showed induction of LC3-II levels, autophagosome formation and autophagy fluxes in DUSP5-deficient NHEKs. Furthermore, Western analysis demonstrated specific induction of phosphorylated ERK in DUSP5-deficient and HPV16 E7-expressing NHEKs, indicating that HPV16 E7-mediated repression of DUSP5 results in induced MAPK/ERK signaling. Finally, phosphorylated mTOR and ULK (S757) were reduced in DUSP5-deficient NHEKs, while phosphorylated ULK (S555) and AMPK were increased, thereby inducing canonical autophagy through the mTOR and AMPK pathways. In conclusion, our results demonstrate that HPV16 E7 expression reduces DUSP5 levels, which in turn results in active MAPK/ERK signaling and induction of canonical autophagy through mTOR and MAPK regulation. Given its demonstrated inverse correlation with clinical cancerous tissues, DUSP5 may serve as a potential therapeutic target for cervical cancer.
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http://dx.doi.org/10.1155/2022/1863098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966754PMC
April 2022

Novel Synthetic Lipopeptides as Potential Mucosal Adjuvants Enhanced SARS-CoV-2 rRBD-Induced Immune Response.

Front Immunol 2022 9;13:833418. Epub 2022 Mar 9.

National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing, China.

As TLR2 agonists, several lipopeptides had been proved to be candidate vaccine adjuvants. In our previous study, lipopeptides mimicking N-terminal structures of the bacterial lipoproteins were also able to promote antigen-specific immune response. However, the structure-activity relationship of lipopeptides as TLR2 agonists is still unclear. Here, 23 synthetic lipopeptides with the same lipid moiety but different peptide sequences were synthesized, and their TLR2 activities and mucosal adjuvant effects to OVA were evaluated. LP1-14, LP1-30, LP1-34 and LP2-2 exhibited significantly lower cytotoxicity and stronger TLR2 activity compared with PamCSK, the latter being one of the most potent TLR2 agonists. LP1-34 and LP2-2 assisted OVA to induce more profound specific IgG in sera or sIgA in BALF than PamCSK. Furthermore, the possibility of LP1-34, LP2-2 and PamCSK as the mucosal adjuvant for the SARS-CoV-2 recombinant RBD (rRBD) was investigated. Intranasally immunized with rRBD plus either the novel lipopeptide or PamCSK significantly increased the levels of specific serum and respiratory mucosal IgG and IgA, while rRBD alone failed to induce specific immune response due to its low immunogenicity. The novel lipopeptides, especially LP2-2, significantly increased levels of rRBD-induced SARS-CoV-2 neutralizing antibody in sera, BALF and nasal wash. Finally, Support vector machine (SVM) results suggested that charged residues in lipopeptides might be beneficial to the agonist activity, while lipophilic residues might adversely affect the agonistic activity. Figuring out the relationship between peptide sequence in the lipopeptide and its TLR2 activity may lay the foundation for the rational design of novel lipopeptide adjuvant for COVID-19 vaccine.
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http://dx.doi.org/10.3389/fimmu.2022.833418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959576PMC
March 2022
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