Publications by authors named "Hang Zhang"

672 Publications

Posterior cruciate ligament reconstruction with independent internal brace reinforcement: surgical technique and clinical outcomes with a minimum two year follow-up.

Int Orthop 2022 May 26. Epub 2022 May 26.

Department of Orthopedics, Joint Surgery and Sports Medicine, First Affiliated Hospital of China Medical University, 155 Nanjing North StreetLiaoning Province, Shenyang, 110001, People's Republic of China.

Purpose: We developed an augmentation technique for PCL reconstruction with independent internal brace reinforcement and evaluated the functional outcome after PCL reconstruction employing autologous hamstrings augmented with an internal brace system for patients with isolated or combined grade 3 posterior instability who were treated with this technique.

Methods: From January 2016 to January 2018, patients with isolated or combined grade 3 PCL tears who underwent single-bundle PCL reconstruction using autologous hamstrings augmented with independent internal braces were studied. The function of the operated knee was evaluated according to the International Knee Documentation Committee (IKDC) score, Lysholm score, and Tegner activity score. The patients were asked the level of returned to their previous sport. Posterior knee laxity was examined with a KT-1000 arthrometer, and data on range of motion (ROM), re-operation, and other complications were collected.

Results: A total of 33 consecutive patients who received single-bundle PCL reconstruction using autologous hamstrings augmented with independent internal braces with a minimum two years follow-up were included in this study. Two patients had undergone this procedure during the study period and were not included in this study (one had combined bone fractures, and one patient had previous meniscus surgery). Thirty-one patients were available for final analysis. The mean follow-up was 45.35 ± 10.88 months (range 29-66 months). The average IKDC subjective knee evaluation scores from 51.65 ± 12.35 to 84.52 ± 6.42, the Lysholm score from 53.90 ± 11.86 to 85.68 ± 4.99, and the Tegner score from 2.81 ± 0.79 to 6.71 ± 1.83 (P < 0.05 for all). The mean total posterior side-to-side difference in knee laxity, assessed using a KT-1000 arthrometer, decreased from 12.13 ± 2.66 mm pre-operatively to 1.87 ± 0.56 mm post-operatively at 70° (P < 0.05). Most patients (29/31) had normal or near normal knee ROM post-operatively; two patients revealed a 6-15° loss of knee flexion compared with the contralateral knee. Twenty-nine patients (93.55%) returned to a normal daily exercise level. Twenty-three patients (74.19%) returned to competitive sports with high-level sports (Tegner score of 6 or above; eleven patients (35.48%) reported to be on the same level as well as the Tegner level); six patients (19.35%) returned to recreational sports (Tegner score of 4 or 5). Two patients had Tegner scores of 2 and 3, indicating poor function level. No patient needed PCL revision surgery during the follow-up period.

Conclusion: Single-bundle PCL reconstruction with internal brace augmentation for PCL injury exhibited satisfactory posterior stability and clinical outcomes in patients with isolated or combined grade 3 PCL injuries at a minimum two year follow-up.
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http://dx.doi.org/10.1007/s00264-022-05448-4DOI Listing
May 2022

Correction: LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells.

Oncotarget 2022 19;13:694. Epub 2022 May 19.

Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

[This corrects the article DOI: 10.18632/oncotarget.16880.].
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http://dx.doi.org/10.18632/oncotarget.28216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119686PMC
May 2022

Ball Milling Solid-state Synthesis of Highly Crystalline Prussian Blue Analogue Na2-xMnFe(CN)6 Cathodes for All-climate Sodium-ion Batteries.

Angew Chem Int Ed Engl 2022 May 18. Epub 2022 May 18.

Wenzhou University, Wenzhou, Wenzhou, CHINA.

With a series of merits, Prussian blue analogs (PBAs) have been considered superior cathode materials for sodium-ion batteries (SIBs). Their commercialization, however, still suffers from inferior stability, considerable [Fe(CN) 6 ] defects and interstitial water in the framework, which are related to the rapid crystal growth. Herein, a "water-in-salt" nanoreactor is proposed to synthesize highly crystallized PBAs with decreased defects and water, which show both superior specific capacity and rate capability in SIBs. The air-stability, all-climate, and full-cell properties of our PBA have also been evaluated, and it exhibits enhanced electrochemical performance and higher volume yield than its counterpart synthesized via the water-based co-precipitation method. Furthermore, their highly reversible sodium-ion storage behavior has been measured and identified via multiple in situ techniques. This work could pave the way for the PBA-based SIBs in grid-scale energy storage systems.
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http://dx.doi.org/10.1002/anie.202205867DOI Listing
May 2022

An Enhanced Photothermal Therapeutic Iridium Hybrid Platform Reversing the Tumor Hypoxic Microenvironment.

Molecules 2022 Apr 19;27(9). Epub 2022 Apr 19.

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.

As hypoxia is closely associated with tumor progression, proliferation, invasion, metastasis, and strong resistance to therapy, regulating and overcoming the hypoxia tumor microenvironment are two increasingly important aspects of tumor treatment. Herein, we report a phototherapeutic platform that uses the organic photosensitizer diketopyrrolopyrrole (DPP) derivative and inorganic iridium salts (IrCl) with photothermal activity and the capacity to decompose HO efficiently. The characterization of their photophysical properties proved that DPP-Ir nanoparticles are capable of remarkable near-infrared (NIR) absorption, and compared to DPP nanoparticles, the photothermal conversion efficiency (PCE) increases from 42.1% in DPP nanoparticles to 67.0% in DPP-Ir nanoparticles. The hybrid nanoparticles utilize the catalytic decomposition of endogenous HO to produce oxygen for the downregulation of the hypoxia-inducible factor 1 subunit alpha (HIF-1α) protein, which could reverse the tumor hypoxic microenvironment. Benefiting from the excellent optical properties and good biocompatibility, the hybrid platform exhibits efficient photothermal therapeutic effects as well as good biological safety. In conclusion, such a hybrid platform could improve photothermal therapy against cancer.
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http://dx.doi.org/10.3390/molecules27092629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101055PMC
April 2022

LRP4 is required for the olfactory association task in the piriform cortex.

Cell Biosci 2022 May 7;12(1):54. Epub 2022 May 7.

Laboratory of Synaptic Development and Plasticity, Institute of Life Science and School of Life Sciences, Nanchang University, Nanchang, 330031, Jiangxi, China.

Background: Low-density lipoprotein receptor-related protein 4 (LRP4) plays a critical role in the central nervous system (CNS), including hippocampal synaptic plasticity, maintenance of excitatory synaptic transmission, fear regulation, as well as long-term potentiation (LTP).

Results: In this study, we found that Lrp4 was highly expressed in layer II of the piriform cortex. Both body weight and brain weight decreased in Lrp4 mice without TMD (Transmembrane domain) and ICD (intracellular domain) of LRP4. However, in the piriform cortical neurons of Lrp4 mice, the spine density increased, and the frequency of both mEPSC (miniature excitatory postsynaptic current) and sEPSC (spontaneous excitatory postsynaptic current) was enhanced. Intriguingly, finding food in the buried food-seeking test was prolonged in both Lrp4 mice and Lrp4 cKO (conditional knockout of Lrp4 in the piriform cortex) mice.

Conclusions: This study indicated that the full length of LRP4 in the piriform cortex was necessary for maintaining synaptic plasticity and the integrity of olfactory function.
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http://dx.doi.org/10.1186/s13578-022-00792-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080164PMC
May 2022

Self-harm and Aggression in Chinese Early Adolescents: Their Co-occurrence and the Role of Bullying Victimization.

J Youth Adolesc 2022 May 3. Epub 2022 May 3.

Collaborative Innovation Center of Assessment toward Basic Education Quality, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing, 100875, China.

Self-harm and aggression increase markedly during early adolescence. However, few studies considered these harmful behaviors simultaneously. This study employed a person-centered approach to identify profiles of adolescents who differed in their patterns of self-harm, reactive aggression, and proactive aggression, examined the stability of these patterns, and explored the effect of bullying victimization on latent profile membership and transition. A total of 2463 early adolescents (48.8% girls, M = 13.93 ± 0.59) participated in two waves of the study over six months. The results indicated that low symptoms profile (80.4%), moderate aggression profile (14.2%), high aggression profile (3.0%), and high self-harm profile (2.4%) were identified at time 1, and low symptoms profile (82.1%), dual-harm profile (7.6%), high aggression profile (7.7%), and high self-harm profile (2.6%) were identified at time 2. Adolescents assigned to at-risk profiles showed moderate to high transition, suggesting the developmental heterogeneity of self-harm and aggression. Moreover, adolescents high in bullying victimization were more likely to belong or transition to at-risk profiles. The findings revealed the co-occurring and transitional nature of self-harm and aggression and the transdiagnostic role of bullying victimization, which can be used to guide prevention and intervention strategies.
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http://dx.doi.org/10.1007/s10964-022-01620-6DOI Listing
May 2022

Isolation of Mouse Primary Microglia by Magnetic-activated Cell Sorting in Animal Models of Demyelination.

J Vis Exp 2022 04 5(182). Epub 2022 Apr 5.

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology;

Microglia, the resident innate immune cells in the brain, are the primary responders to inflammation or injury in the central nervous system (CNS). Microglia can be divided into resting state and activated state and can rapidly change state in response to the microenvironment of the brain. Microglia will be activated under different pathological conditions and exhibit different phenotypes. In addition, there are many different subgroups of activated microglia and great heterogeneity between different subgroups. The heterogeneity mainly depends on the molecular specificity of microglia. Studies have revealed that microglia will be activated and play an important role in the pathological process of inflammatory demyelination. To better understand the characteristics of microglia in inflammatory demyelinating diseases such as multiple sclerosis and neuromyelitis optica spectrum disorder, we propose a perilesional primary microglial sorting protocol. This protocol utilizes columnar magnetic-activated cell sorting (MACS) to obtain highly purified primary microglia and preserve the molecular characteristics of microglia to investigate the potential effects of microglia in inflammatory demyelinating diseases.
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http://dx.doi.org/10.3791/63511DOI Listing
April 2022

Preclinical Pharmacokinetic Studies of a Novel Diuretic Inhibiting Urea Transporters.

Molecules 2022 Apr 11;27(8). Epub 2022 Apr 11.

State Key Laboratory of Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.

Urea transporter (UT) inhibitors are a class of promising novel diuretics that do not cause the imbalance of Na, K, Cl, and other electrolytes. In our previous studies, , a promising diuretic candidate inhibiting UT, was discovered and showed potent diuretic activities in rodents. Here, a sensitive liquid chromatography-tandem mass spectrometry method for the quantitation of in rat plasma, urine, feces, bile, and tissue homogenates was developed and validated to support the preclinical pharmacokinetic studies. The tissue distribution, excretion, and plasma protein binding were investigated in rats. After a single oral dose of at 25, 50, and 100 mg/kg, the drug exposure increased linearly with the dose. The drug accumulation was observed after multiple oral doses compared to a single dose. In the distribution study, exhibited a wide distribution to tissues with high blood perfusion, such as kidney, heart, lung, and spleen, and the lowest distribution in the brain and testis. The accumulative excretion rate of was 0.14%, 3.16%, and 0.018% in urine, feces, and bile, respectively. The plasma protein binding of was approximately 60% in rats and 40% in humans. This is the first study on the preclinical pharmacokinetic profiles of .
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http://dx.doi.org/10.3390/molecules27082451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027532PMC
April 2022

Bioinspired Design of seco-Chlorin Photosensitizers to Overcome Phototoxic Effects in Photodynamic Therapy.

Angew Chem Int Ed Engl 2022 Apr 20:e202204330. Epub 2022 Apr 20.

Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.

Photodynamic therapy (PDT) is a non-invasive treatment modality against a range of cancers and nonmalignant diseases, however one must be aware of the risk of causing phototoxic reactions after treatment. We herein report a bioinspired design of next-generation photosensitizers (PSs) that not only effectively produce ROS but undergo fast metabolism after treatment to overcome undesirable side effects. We constructed a series of β-pyrrolic ring-opening seco-chlorins, termed beidaphyrin (BP), beidapholactone (BPL), and their zinc(II) derivatives (ZnBP and ZnBPL), featuring intense near-infrared absorption and effective O photosensitization. Irradiation of ZnBPL led to a non-cytotoxic, metabolizable beidaphodiacetamide (ZnBPD) via in situ generated O but not O , as revealed by mechanistic studies including time-resolved absorption, kinetics, and isotope labeling. Furthermore, water-soluble ZnBPL showed an effective therapeutic outcome, fast metabolism, and negligible phototoxic reactions.
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http://dx.doi.org/10.1002/anie.202204330DOI Listing
April 2022

Correction to: miR-223-3p carried by cancer-associated fibroblast microvesicles targets SORBS1 to modulate the progression of gastric cancer.

Cancer Cell Int 2022 Apr 20;22(1):159. Epub 2022 Apr 20.

Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, 310009, Zhejiang, China.

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http://dx.doi.org/10.1186/s12935-022-02571-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022354PMC
April 2022

Bipolar fluorinated covalent triazine framework cathode with high lithium storage and long cycling capability.

RSC Adv 2022 Apr 13;12(18):11484-11491. Epub 2022 Apr 13.

School of Chemistry and Chemical Engineering, Jiangxi Province Engineering Research Center of Ecological Chemical Industry, Jiujiang University Jiujiang Jiangxi 332005 China

Organic materials with adjustable structures and wide sources are expected to become potential candidates for commercial cathodes of lithium-ion batteries (LIBs). However, most organic materials have unstable structures, poor conductivity, and are easily soluble in electrolytes, resulting in unsatisfactory lithium storage performance. Covalent-organic frameworks have attracted extensive attention due to their stable frame structures, adjustable pore structures and functionalized official groups. Herein, a fluorinated covalent triazine framework (FCTF) is synthesized by a simple ion-thermal method. Compared with the fluorine-free covalent triazine frameworks (CTFs), the introduction of fluorine improves the lithium storage performance of CTF. When used as a cathode for lithium ion batteries, FCTF can retain a reversible capacity of 125.6 mA h g after 200 cycles at a current density of 100 mA g. Besides, it also delivers 106.3 mA h g after 400 cycles at a current density of 200 mA g with 0.03% decrease per cycle (from 40 to 400 cycles).
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http://dx.doi.org/10.1039/d2ra01582jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006349PMC
April 2022

CDK inhibitor Palbociclib targets STING to alleviate autoinflammation.

EMBO Rep 2022 Apr 11:e53932. Epub 2022 Apr 11.

Clinical and Translational Research Center, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Aberrant activation of stimulator of interferon genes (STING) is tightly associated with multiple types of disease, including cancer, infection, and autoimmune diseases. However, the development of STING modulators for the therapy of STING-related diseases is still an unmet clinical need. We employed a high-throughput screening approach based on the interaction of small-molecule chemical compounds with recombinant STING protein to identify functional STING modulators. Intriguingly, the cyclin-dependent protein kinase (CDK) inhibitor Palbociclib was found to directly bind STING and inhibit its activation in both mouse and human cells. Mechanistically, Palbociclib targets Y167 of STING to block its dimerization, its binding with cyclic dinucleotides, and its trafficking. Importantly, Palbociclib alleviates autoimmune disease features induced by dextran sulphate sodium or genetic ablation of three prime repair exonuclease 1 (Trex1) in mice in a STING-dependent manner. Our work identifies Palbociclib as a novel pharmacological inhibitor of STING that abrogates its homodimerization and provides a basis for the fast repurposing of this Food and Drug Administration-approved drug for the therapy of autoinflammatory diseases.
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http://dx.doi.org/10.15252/embr.202153932DOI Listing
April 2022

Prediction of acute kidney injury after cardiac surgery: model development using a Chinese electronic health record dataset.

J Transl Med 2022 04 9;20(1):166. Epub 2022 Apr 9.

Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, Nanjing Medical University, No. 68 Changle Road, Nanjing, 210006, China.

Background: Acute kidney injury (AKI) is a major complication following cardiac surgery that substantially increases morbidity and mortality. Current diagnostic guidelines based on elevated serum creatinine and/or the presence of oliguria potentially delay its diagnosis. We presented a series of models for predicting AKI after cardiac surgery based on electronic health record data.

Methods: We enrolled 1457 adult patients who underwent cardiac surgery at Nanjing First Hospital from January 2017 to June 2019. 193 clinical features, including demographic characteristics, comorbidities and hospital evaluation, laboratory test, medication, and surgical information, were available for each patient. The number of important variables was determined using the sliding windows sequential forward feature selection technique (SWSFS). The following model development methods were introduced: extreme gradient boosting (XGBoost), random forest (RF), deep forest (DF), and logistic regression. Model performance was accessed using the area under the receiver operating characteristic curve (AUROC). We additionally applied SHapley Additive exPlanation (SHAP) values to explain the RF model. AKI was defined according to Kidney Disease Improving Global Outcomes guidelines.

Results: In the discovery set, SWSFS identified 16 important variables. The top 5 variables in the RF importance matrix plot were central venous pressure, intraoperative urine output, hemoglobin, serum potassium, and lactic dehydrogenase. In the validation set, the DF model exhibited the highest AUROC (0.881, 95% confidence interval [CI] 0.831-0.930), followed by RF (0.872, 95% CI 0.820-0.923) and XGBoost (0.857, 95% CI 0.802-0.912). A nomogram model was constructed based on intraoperative longitudinal features, achieving an AUROC of 0.824 (95% CI 0.763-0.885) in the validation set. The SHAP values successfully illustrated the positive or negative contribution of the 16 variables attributed to the output of the RF model and the individual variable's effect on model prediction.

Conclusions: Our study identified 16 important predictors and provided a series of prediction models to enhance risk stratification of AKI after cardiac surgery. These novel predictors might aid in choosing proper preventive and therapeutic strategies in the perioperative management of AKI patients.
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http://dx.doi.org/10.1186/s12967-022-03351-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8994277PMC
April 2022

Laparoscopic versus open pancreaticoduodenectomy for pancreatic ductal adenocarcinoma: study protocol for a multicentre randomised controlled trial.

BMJ Open 2022 04 4;12(4):e057128. Epub 2022 Apr 4.

Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

Introduction: Pancreatic cancer is one of the deadliest cancers and pancreaticoduodenectomy (PD) is recommended as the optimal operation for resectable pancreatic head cancer. Minimally invasive surgery, which initially emerged as hybrid-laparoscopy and recently developed into total laparoscopy surgery, has been widely used for various abdominal surgeries. However, controversy persists regarding whether laparoscopic PD (LPD) is inferior to open PD (OPD) for resectable pancreatic ductal adenocarcinoma (PDAC) treatment. Further studies, especially randomised clinical trials, are warranted to compare these two surgical techniques.

Methods And Analysis: The TJDBPS07 study is designed as a prospective, randomised controlled, parallel-group, open-label, multicentre noninferiority study. All participating pancreatic surgical centres comprise specialists who have performed no less than 104 LPDs and OPDs, respectively. A total of 200 strictly selected PD candidates diagnosed with PDAC will be randomised to receive LPD or OPD. The primary outcome is the 5-year overall survival rate, whereas the secondary outcomes include overall survival, disease-free survival, 90-day mortality, complication rate, comprehensive complication index, length of stay and intraoperative indicators. We hypothesise that LPD is not inferior to OPD for the treatment of resectable PDAC. The enrolment schedule is estimated to be 2 years and follow-up for each patient will be 5 years.

Ethics And Dissemination: This study received approval from the Tongji Hospital Ethics Committee of Tongji Medical College, Huazhong University of Science and Technology, and monitor from an independent third-party organisation. Results of this trial will be presented in international meetings and published in a peer-reviewed journal.

Trial Registration Number: NCT03785743.
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http://dx.doi.org/10.1136/bmjopen-2021-057128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981294PMC
April 2022

Tea polyphenol/glycerol-treated double-network hydrogel with enhanced mechanical stability and anti-drying, antioxidant and antibacterial properties for accelerating wound healing.

Int J Biol Macromol 2022 May 26;208:530-543. Epub 2022 Mar 26.

School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China. Electronic address:

Frequent dressing changes can result in secondary wound damage. Therefore, it is of great significance to construct a wound dressing that can be used for a long time without changing. Here, a double-network hydrogel was synthesized through hydrogen bonding interactions of tea polyphenol (TP)/glycerol with photo-crosslinked N-acryloyl glycinamide (NAGA), gelatin methacrylate (GelMA), and nanoclay hydrogel. The glycerol/water solvent slowed the diffusion of TP into the NAGA/GelMA/Laponite (NGL)hydrogel, thereby avoiding excessive crosslinking, and forming a uniform network. The hydrogel exhibited excellent water retention (84% within 28 days). Additionally, due to the hygroscopicity of glycerol, the hydrogel's mechanical strength (0.73-1.14 MPa) and tensile strain (207%-353%) increased further after 14 days in an open environment. Additionally, the hydrogel exhibited superior anti-ultraviolet and antioxidant properties, which effectively alleviated the wound site's oxidative stress and accelerated wound healing. Moreover, antibacterial activity was observed against both E. coli and S. aureus in the hydrogel wound dressing. Thus, by promoting wound closure, angiogenesis and collagen deposition, the double-network NGLG20/TG hydrogel dressing can successfully accelerate wound healing. The multifunctional double-network hydrogel, therefore, shows immense potential as an ideal candidate for wound dressings because it is long-lasting and prevents secondary damage caused by frequent dressing changes.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.03.128DOI Listing
May 2022

Leptin-Induced HLA-G Inhibits Myometrial Contraction and Differentiation.

Cells 2022 03 10;11(6). Epub 2022 Mar 10.

UMR 1231, Lipides Nutrition Cancer, INSERM, F-21000 Dijon, France.

Maternal obesity is associated with a wide spectrum of labour disorders, including preterm birth. Leptin, a pro-inflammatory adipokine and a key factor of obesity, is suspected to play a major role in these disorders. OB-R, its receptor, is expressed on macrophages and myocytes, two cell types critical for labour onset. Macrophages secrete reactive oxygen species/pro-inflammatory cytokines, responsible for myometrial differentiation while myocytes control uterine contractions. In this study, we assessed the effect of leptin on myometrial contraction and differentiation using our validated co-culture model of human primary macrophages and myocytes. We demonstrated that leptin had a different effect on myocytes and macrophages depending on the dose. A low leptin concentration induced a tocolytic effect by preventing myocytes' contraction, differentiation, and macrophage-induced ROS production. Additionally, leptin led to an increase in HLA-G expression, suggesting that the tocolytic effect of leptin may be driven by HLA-G, a tolerogenic molecule. Finally, we observed that recombinant HLA-G also prevented LPS-induced ROS production by macrophages. Altogether, these data provide a putative molecular mechanism by which leptin may induce immune tolerance and therefore interfere with labour-associated mechanisms. Therefore, HLA-G represents a potential innovative therapeutic target in the pharmacological management of preterm labour.
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http://dx.doi.org/10.3390/cells11060954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946078PMC
March 2022

LncRNA SNHG11 enhances bevacizumab resistance in colorectal cancer by mediating miR-1207-5p/ABCC1 axis.

Anticancer Drugs 2022 Jul 23;33(6):575-586. Epub 2022 Mar 23.

Cancer Center, Huizhou First Hospital, Huizhou.

Long noncoding RNAs (lncRNAs) have been reported to serve as vital regulators in the chemoresistance of human cancers, including colorectal cancer (CRC). In this study, we aimed to explore the functions of lncRNA small nucleolar RNA host gene 11 (SNHG11) in the resistance of CRC to bevacizumab. Quantitative real-time PCR, western blot assay or immunohistochemistry assay were performed to examine the expression of SNHG11, microRNA-1207-5p (miR-1207-5p), ATP binding cassette subfamily C member 1 (ABCC1) and Ki67. Cell Counting Kit-8 assay was conducted to evaluate bevacizumab resistance and cell viability. 5'-ethynyl-2'-deoxyuridine analysis, flow cytometry analysis and wound-healing assay were conducted for cell proliferation, apoptosis and migration, respectively. Dual-luciferase reporter assay and RNA immunoprecipitation assay were employed to analyze the relations among SNHG11, miR-1207-5p and ABCC1. Murine xenograft model assay was employed to analyze bevacizumab resistance in vivo. The exosomes were observed under transmission electron microscopy. SNHG11 was overexpressed in bevacizumab-resistant CRC tissues and cells. Knockdown of SNHG11 restrained bevacizumab resistance, repressed cell proliferation and migration, and promoted apoptosis in bevacizumab-resistant CRC cells. MiR-1207-5p served as the target of SNHG11 and SNHG11 regulated bevacizumab resistance by targeting miR-1207-5p. ABCC1 was the target gene of miR-1207-5p. Overexpression of miR-1207-5p inhibited bevacizumab resistance and cell progression in bevacizumab-resistant CRC cells, with ABCC1 elevation abrogated the impacts. SNHG11 silencing repressed bevacizumab resistance in vivo. In addition, exosomal SNHG11 was upregulated in bevacizumab-resistant CRC cells. SNHG11 contributes to bevacizumab resistance in CRC depending on the modulation of miR-1207-5p and ABCC1.
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http://dx.doi.org/10.1097/CAD.0000000000001289DOI Listing
July 2022

Brain oxygen extraction and neural tissue susceptibility are associated with cognitive impairment in older individuals.

J Neuroimaging 2022 Mar 16. Epub 2022 Mar 16.

MRI Research Institute, Department of Radiology, Weill Cornell Medicine, New York, New York, USA.

Background And Purpose: We investigated the effects of aging, white matter hyperintensities (WMH), and cognitive impairment on brain iron levels and cerebral oxygen metabolism, known to be altered in Alzheimer's disease (AD), using quantitative susceptibility mapping and MR-based cerebral oxygen extraction fraction (OEF).

Methods: In 100 individuals over the age of 50 (68/32 cognitively impaired/intact), OEF and neural tissue susceptibility (χ ) were computed retrospectively from MRI multi-echo gradient echo data, obtained on a 3 Tesla MRI scanner. The effects of age and WMH on OEF and χ were assessed within groups, and OEF and χ were assessed between groups, using multivariate regression analyses.

Results: Cognitively impaired subjects were found to have 19% higher OEF and 34% higher χ than cognitively intact subjects in the cortical gray matter and several frontal, temporal, and parietal regions (p < .05). Increased WMH burden was significantly associated with decreased OEF in the cognitively impaired, but not in the cognitively intact. Older age had a stronger association with decreased OEF in the cognitively intact group. Both older age and increased WMH burden were significantly associated with increased χ in temporoparietal regions in the cognitively impaired.

Conclusions: Higher brain OEF and χ in cognitively impaired older individuals may reflect altered oxygen metabolism and iron in areas with underlying AD pathology. Both age and WMH have associations with OEF and χ but are modified by the presence of cognitive impairment.
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http://dx.doi.org/10.1111/jon.12990DOI Listing
March 2022

Attosecond-Resolved Coherent Control of Lattice Vibrations in Thermoelectric SnSe.

J Phys Chem Lett 2022 Mar 15;13(11):2584-2590. Epub 2022 Mar 15.

Laboratory for Shock Wave and Detonation Physics, Institute of Fluid Physics, China Academy of Engineering Physics, Mianyang 621900, China.

Manipulating lattice vibrations is the cornerstone to achieving ultralow thermal conductivity in thermoelectrics. Although spatial control by novel material designs has been recently reported, temporal manipulation, which can shape thermoelectric properties under nonequilibrium conditions, remains largely unexplored. Here, taking SnSe as a representative, we have demonstrated that in the ultrafast pump-pump-probe spectroscopy, electronic and lattice coherences inherited from optical excitations can be exploited independently to manipulate phonon oscillations in a highly selective manner. Specifically, when the pump-pump delay time () is in the electronic coherence time range, the amplitude, frequency, and lifetime of all phonon modes are simultaneously following the optical cycle. While extending into the lattice coherence time range, the amplitude of each coherent phonon mode can be selectively manipulated according to its intrinsic period without changing the frequency and lifetime. This work opens up exciting avenues to temporally and discriminatorily manipulate phononic processes in thermoelectric materials.
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http://dx.doi.org/10.1021/acs.jpclett.2c00426DOI Listing
March 2022

The Effect of the Supervisor-Student Relationship on Academic Procrastination: The Chain-Mediating Role of Academic Self-Efficacy and Learning Adaptation.

Int J Environ Res Public Health 2022 02 24;19(5). Epub 2022 Feb 24.

School of Psychology, Henan University, Kaifeng 475004, China.

This study used a sample of 818 postgraduate students across several universities in China, to explore the effect of the supervisor-student relationship on procrastination in postgraduates, and the mediating roles played by the postgraduates' academic self-efficacy and learning adaptation. The study employs multiple scales and finds that: (1) the relationship between postgraduates and their supervisors is significantly and negatively correlated with academic procrastination; (2) the academic self-efficacy of postgraduates plays an independent intermediary role in the connection between the supervisor-student relationship and academic procrastination; (3) the learning adaptation of postgraduates also plays an independent intermediary role in the connection between the supervisor-student relationship and academic procrastination; (4) the academic self-efficacy and learning adaptation of postgraduates shows a chain-mediating effect in the connection between the supervisor-student relationship and academic procrastination. Ultimately, the supervisor-student relationship is an important factor that can directly affect academic procrastination, even if postgraduate students display academic self-efficacy and learning adaptation.
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http://dx.doi.org/10.3390/ijerph19052621DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909498PMC
February 2022

Occurrence of Mycosporine-like Amino Acids (MAAs) from the Bloom-Forming Cyanobacteria Strains.

Molecules 2022 Mar 7;27(5). Epub 2022 Mar 7.

College of Life and Environmental Sciences, Wenzhou University, Wenzhou 325000, China.

Mycosporine-like amino acids (MAAs) are widespread in various microbes and protect them against harsh environments. Here, four different species were isolated from severely eutrophic waterbodies, Lake Dianchi and the Guanqiao fishpond. Morphological characters and molecular phylogenetic analysis verified that the CHAB5919, 5921, and 5926 strains belonged to the clade while Guanqiao01 belonged to the gracile clade. Full wavelength scanning proved that there was obvious maximal absorption at 334 nm through purified methanol extraction, and these substances were further analyzed by HPLC and UPLC-MS-MS. The results showed that two kinds of MAAs were discovered in the cultured strains. One molecular weight was 333.28 and the other was 347.25, and the daughter fragment patterns were in accordance with the previously articles reported shinorine and porphyra-334 ion characters. The concentration of the MAAs was calibrated from semi-prepared MAAs standards from dry cells of PCC7806 algal powder, and the purity of shinorine and porphyra-334 were 90.2% and 85.4%, respectively. The average concentrations of shinorine and porphyra-334 were 0.307-0.385 µg/mg and 0.111-0.136 µg/mg in species, respectively. And there was only one kind of MAAs (shinorine) in species.,with a content of 0.003-0.049 µg/mg dry weight among all strains. The shinorine concentration in was higher than that in strains. The total MAAs production can be ranked as > .
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http://dx.doi.org/10.3390/molecules27051734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911825PMC
March 2022

Postsynaptic Potential Energy as Determinant of Synaptic Plasticity.

Front Comput Neurosci 2022 17;16:804604. Epub 2022 Feb 17.

School of Automation, Central South University, Changsha, China.

Metabolic energy can be used as a unifying principle to control neuronal activity. However, whether and how metabolic energy alone can determine the outcome of synaptic plasticity remains unclear. This study proposes a computational model of synaptic plasticity that is completely determined by energy. A simple quantitative relationship between synaptic plasticity and postsynaptic potential energy is established. Synaptic weight is directly proportional to the difference between the baseline potential energy and the suprathreshold potential energy and is constrained by the maximum energy supply. Results show that the energy constraint improves the performance of synaptic plasticity and avoids setting the hard boundary of synaptic weights. With the same set of model parameters, our model can reproduce several classical experiments in homo- and heterosynaptic plasticity. The proposed model can explain the interaction mechanism of Hebbian and homeostatic plasticity at the cellular level. Homeostatic synaptic plasticity at different time scales coexists. Homeostatic plasticity operating on a long time scale is caused by heterosynaptic plasticity and, on the same time scale as Hebbian synaptic plasticity, is caused by the constraint of energy supply.
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http://dx.doi.org/10.3389/fncom.2022.804604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891168PMC
February 2022

QSMRim-Net: Imbalance-aware learning for identification of chronic active multiple sclerosis lesions on quantitative susceptibility maps.

Neuroimage Clin 2022 1;34:102979. Epub 2022 Mar 1.

Penn Statistics in Imaging and Visualization Endeavor (PennSIVE) Center, Department of Biostatistics, Epidemiology, and Informatics, University of Pennsylvania, Philadelphia, PA, USA. Electronic address:

Background And Purpose: Chronic active multiple sclerosis (MS) lesions are characterized by a paramagnetic rim at the edge of the lesion and are associated with increased disability in patients. Quantitative susceptibility mapping (QSM) is an MRI technique that is sensitive to chronic active lesions, termed rim + lesions on the QSM. We present QSMRim-Net, a data imbalance-aware deep neural network that fuses lesion-level radiomic and convolutional image features for automated identification of rim + lesions on QSM.

Methods: QSM and T2-weighted-Fluid-Attenuated Inversion Recovery (T2-FLAIR) MRI of the brain were collected at 3 T for 172 MS patients. Rim + lesions were manually annotated by two human experts, followed by consensus from a third expert, for a total of 177 rim + and 3986 rim negative (rim-) lesions. Our automated rim + detection algorithm, QSMRim-Net, consists of a two-branch feature extraction network and a synthetic minority oversampling network to classify rim + lesions. The first network branch is for image feature extraction from the QSM and T2-FLAIR, and the second network branch is a fully connected network for QSM lesion-level radiomic feature extraction. The oversampling network is designed to increase classification performance with imbalanced data.

Results: On a lesion-level, in a five-fold cross validation framework, the proposed QSMRim-Net detected rim + lesions with a partial area under the receiver operating characteristic curve (pROC AUC) of 0.760, where clinically relevant false positive rates of less than 0.1 were considered. The method attained an area under the precision recall curve (PR AUC) of 0.704. QSMRim-Net out-performed other state-of-the-art methods applied to the QSM on both pROC AUC and PR AUC. On a subject-level, comparing the predicted rim + lesion count and the human expert annotated count, QSMRim-Net achieved the lowest mean square error of 0.98 and the highest correlation of 0.89 (95% CI: 0.86, 0.92).

Conclusion: This study develops a novel automated deep neural network for rim + MS lesion identification using T2-FLAIR and QSM images.
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http://dx.doi.org/10.1016/j.nicl.2022.102979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8892132PMC
May 2022

Sequence Analysis of and Its Role in the Interferon Pathway.

Front Vet Sci 2022 15;9:805301. Epub 2022 Feb 15.

Henan University of Animal Husbandry and Economy, Zhengzhou, China.

Monkey diseases are becoming increasingly severe, and some may be transmitted to humans through direct and indirect contact. Innate immunity is the first line of defense against foreign microorganisms. It is of great significance to explore the immune characteristics of monkey and human diseases. TRIM4, an important immune molecule in , was cloned and its immunological characteristics were preliminarily explored. The results showed that was in the same branch as human . Overexpression of TRIM4 increased the mRNA levels of interferon (IFN)-, TRIM4 up-regulated the activities of IFN-beta, NF-κB, and ISRE reporter. In contrast, inhibiting expression by small interfering RNA (siRNA) down-regulated the IFN pathway. In summary, plays an essential role in the IFN pathway.
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http://dx.doi.org/10.3389/fvets.2022.805301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8887992PMC
February 2022

Efficacy and safety of long-term transcutaneous electroacupuncture versus sham transcutaneous electroacupuncture for delayed gastric emptying after distal gastrectomy: study protocol for a randomized, patient-assessor blinded, controlled trial.

Trials 2022 Mar 3;23(1):189. Epub 2022 Mar 3.

Department of General Surgery, Shang-Yu branch of SAHZU, School of Medicine, No. 517 Shi-Min Road, Shaoxing, 312300, China.

Background: Delayed gastric emptying (DGE) after distal gastrectomy impacts patients' nutritional status and quality of life. The current treatments of DGE seem unsatisfactory or need invasive interventions. It is unknown whether transcutaneous electroacupuncture (TEA) is effective in treating DGE.

Methods: A total of 90 eligible participants who underwent distal gastrectomy will be randomly allocated to either the TEA group (n = 60) or the sham transcutaneous electroacupuncture (sham-TEA) group (n = 30). Each participant will receive TEA on the bilateral acupoints of Zusanli (ST36) and Neiguan (PC6) for 4 weeks. The primary outcomes will be the residual rates of radioactivity in the stomach by gastric scintigraphy and total response rates. The secondary outcomes will be endoscopic features, autonomic function, nutritional and psychological status, serum examination, and quality of life (QoL). The adverse events will also be reported. The patients will be followed up 1 year after the treatment.

Discussion: The findings of this randomized trial will provide high-quality evidence regarding the efficacy and safety of long-term TEA for treating DGE after distal gastrectomy.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2000033965. Registered on 20 June 2020.
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http://dx.doi.org/10.1186/s13063-022-06108-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895584PMC
March 2022

Human Fecal Microbiota Transplantation Reduces the Susceptibility to Dextran Sulfate Sodium-Induced Germ-Free Mouse Colitis.

Front Immunol 2022 14;13:836542. Epub 2022 Feb 14.

College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.

In clinical practice, fecal microbiota transplantation (FMT) has been used to treat inflammatory bowel disease (IBD), and has shown certain effects. However, the selection of FMT donors and the mechanism underlying the effect of FMT intervention in IBD require further exploration. In this study, dextran sodium sulfate (DSS)-induced colitis mice were used to determine the differences in the protection of colitis symptoms, inflammation, and intestinal barrier, by FMT from two donors. Intriguingly, pre-administration of healthy bacterial fluid significantly relieved the symptoms of colitis compared to the ulcerative colitis (UC) bacteria. In addition, healthy donor (HD) bacteria significantly reduced the levels of inflammatory markers Myeloperoxidase (MPO) and Eosinophil peroxidase (EPO), and various pro-inflammatory factors, in colitis mice, and increased the secretion of the anti-inflammatory factor IL-10. Metagenomic sequencing indicated higher species diversity and higher abundance of anti-inflammatory bacteria in the HD intervention group, including , , , short-chain fatty acids (SCFAs)-producing bacterium , and secondary bile acids (SBAs)-producing bacterium . In the UC intervention group, the SCFA-producing bacterium , IBD-related bacterium , , and the conditional pathogen , were more abundant. Metabolomics analysis showed that the two types of FMT significantly modulated the metabolism of DSS-induced mice. Moreover, compared with the UC intervention group, indoleacetic acid and unsaturated fatty acids (DHA, DPA, and EPA) with anti-inflammatory effects were significantly enriched in the HD intervention group. In summary, these results indicate that FMT can alleviate the symptoms of colitis, and the effect of HD intervention is better than that of UC intervention. This study offers new insights into the mechanisms of FMT clinical intervention in IBD.
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http://dx.doi.org/10.3389/fimmu.2022.836542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882623PMC
May 2022

DYRK1B-STAT3 Drives Cardiac Hypertrophy and Heart Failure by Impairing Mitochondrial Bioenergetics.

Circulation 2022 03 2;145(11):829-846. Epub 2022 Mar 2.

Department of Cardiovascular Medicine (L.Z., K..J., Z.L., J.Z., J.H., H.Z., Q.F., C.H., H.X., L.L., W.S., R.Z., K.C., X.Y.), Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.

Background: Heart failure is a global public health issue that is associated with increasing morbidity and mortality. Previous studies have suggested that mitochondrial dysfunction plays critical roles in the progression of heart failure; however, the underlying mechanisms remain unclear. Because kinases have been reported to modulate mitochondrial function, we investigated the effects of DYRK1B (dual-specificity tyrosine-regulated kinase 1B) on mitochondrial bioenergetics, cardiac hypertrophy, and heart failure.

Methods: We engineered DYRK1B transgenic and knockout mice and used transverse aortic constriction to produce an in vivo model of cardiac hypertrophy. The effects of DYRK1B and its downstream mediators were subsequently elucidated using RNA-sequencing analysis and mitochondrial functional analysis.

Results: We found that DYRK1B expression was clearly upregulated in failing human myocardium and in hypertrophic murine hearts, as well. Cardiac-specific DYRK1B overexpression resulted in cardiac dysfunction accompanied by a decline in the left ventricular ejection fraction, fraction shortening, and increased cardiac fibrosis. In striking contrast to DYRK1B overexpression, the deletion of DYRK1B mitigated transverse aortic constriction-induced cardiac hypertrophy and heart failure. Mechanistically, DYRK1B was positively associated with impaired mitochondrial bioenergetics by directly binding with STAT3 to increase its phosphorylation and nuclear accumulation, ultimately contributing toward the downregulation of PGC-1α (peroxisome proliferator-activated receptor gamma coactivator-1α). Furthermore, the inhibition of DYRK1B or STAT3 activity using specific inhibitors was able to restore cardiac performance by rejuvenating mitochondrial bioenergetics.

Conclusions: Taken together, the findings of this study provide new insights into the previously unrecognized role of DYRK1B in mitochondrial bioenergetics and the progression of cardiac hypertrophy and heart failure. Consequently, these findings may provide new therapeutic options for patients with heart failure.
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http://dx.doi.org/10.1161/CIRCULATIONAHA.121.055727DOI Listing
March 2022

Efficacy and safety analysis of bevacizumab combined with capecitabine in the maintenance treatment of RAS-mutant metastatic colorectal cancer.

J Clin Pharm Ther 2022 Apr 25;47(4):531-538. Epub 2022 Feb 25.

Department of Medical Oncology, Huizhou Municipal Central Hospital of Guangdong Province, Huizhou, China.

What Is Known And Objectives: The optimal strategy for maintenance therapy in patients with metastatic colorectal cancer (mCRC) remains controversial. Considering that, beyond progression, co-therapy with bevacizumab and cytotoxic chemotherapy showed less toxicity and a significant disease control rate. We aimed to investigate the differences in efficacy and safety between bevacizumab combined with capecitabine maintenance therapy and capecitabine monotherapy for RAS-mutant mCRC (as defined by mutations in KRAS and NRAS exons 2-4)controlled by bevacizumab plus FOLFIRI chemotherapy for at least 12 weeks.

Methods: We retrospectively analysed patients with RAS-mutant mCRC admitted to the Department of Oncology, Huizhou Municipal Central Hospital from December, 2015 to December, 2020. All patients were first treated with bevacizumab combined with FOLFIRI for at least 12 weeks of induction therapy. 154 patients whose disease was brought under control then continued maintenance therapy. 78 patients were in the observation group (bevacizumab plus capecitabine) and 76 patients were in the control group (capecitabine alone). The efficacy and adverse effects of maintenance treatment were compared between the two groups. The clinicopathological characteristics such as sex, age, performance status (PS) score, primary tumour site, degree of pathological differentiation, baseline carcinoembryonic antigen (CEA) level, microsatellite instability (MSI) status, number of metastatic tumour sites and efficacy of induction treatment were compared in terms of prognosis.

Results And Discussion: The median progression-free survival (mPFS)of patients was 9.0 months (95% CI 8.0-10.0) in the observation group and 7.2 months (95% CI 6.0-8.4) in the control group, with a statistically significant difference (p < 0.05). The baseline CEA level was an independent prognostic factor. Both groups tolerated the toxic side effects.

What Is New And Conclusion: Bevacizumab combined with capecitabine was well tolerated and contributed to a longer PFS time than capecitabine alone, and it is worthy of popularization in clinical practice.
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http://dx.doi.org/10.1111/jcpt.13576DOI Listing
April 2022

Graphene-Supported Naphthalene-Based Polyimide Composite as a High-Performance Sodium Storage Cathode.

ACS Appl Mater Interfaces 2022 Mar 25;14(9):11448-11456. Epub 2022 Feb 25.

Institute for Carbon Neutralization, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang 325035, China.

Electroactive acid anhydride with multicarbonyl is highly promising for electrochemical energy storage because of its high specific capacity and environmental benignity. Its low electrical conductivity and high dissolution in organic electrolyte, however, result in poor cycling and rate capabilities. Here, we report a naphthalene polyimide derivative (NPI) synthesized by using anhydride under condensation polymerization conditions, along with its composite with graphene (NPI-G) fabricated via in situ polymerization. The composite delivers a high reversible capacity and outstanding cycling stability and rate capability as a cathode for sodium-ion batteries (SIBs) owing to the formation of a polymer, the improvement in the electrical conductivity brought about by the highly dispersed graphene sheets, and the enhancement of structural stability resulting from the π-π stacking interaction between the phenyl groups of NPI and the six-member carbon rings of graphene. This investigation sheds light on the development, design, and screening of next-generation organic electrode materials with high performance for SIBs.
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http://dx.doi.org/10.1021/acsami.1c24012DOI Listing
March 2022

Oxidative Stress-Mediated Antibacterial Activity of the Total Flavonoid Extracted from the Ledeb. in Methicillin-Resistant (MRSA).

Vet Sci 2022 Feb 7;9(2). Epub 2022 Feb 7.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

(1) Background: Methicillin-resistant   (MRSA) is a zoonotic pathogen that causes endocarditis, pneumonia, and skin diseases in humans and livestock. (2) Methods: The antibacterial effect of the total flavonoid against MRSA (ATCC43300) extracted from the Ledeb. ( Ledeb) was evaluated by the microdilution method. The oxidative stresses in MRSA were evaluated by the levels of intracellular hydrogen peroxide (HO), reactive oxygen species (ROS), and oxidative stress-related genes. The DNA oxidative damage was tested by the 8-hydroxy-2'-deoxyguanosine (8-OHdG) and DNA gel electrophoresis. The differentially expressed proteins were determined by the method of SDS-PAGE and NanoLC-ESI-MS/MS, while the mRNAs of differential proteins were determined by Real-Time PCR. The changes of ultra-structures in MRSA were observed by Transmission Electron Microscope (TEM). (3) Results: The minimum inhibitory concentration (MIC) of the total flavonoid against MRSA was recorded as 62.5 μg/mL. After treatment with the total flavonoid, the levels of intracellular HO and ROS were increased and the gene expressions against oxidative stress (, , ) were decreased ( < 0.01), while the gene expression for oxidative stress () was increased ( < 0.01). The level of intracellular 8-OHdG in MRSA was increased ( < 0.01) and the DNA was damaged. The results of TEM also showed that the total flavonoid could destroy the ultra-structures in the bacteria. (4) Conclusions: The total flavonoid extracted from the Ledeb can induce the oxidative stress that disturbed the energy metabolism and protein synthesis in MRSA.
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http://dx.doi.org/10.3390/vetsci9020071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874552PMC
February 2022
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