Publications by authors named "Hang Yuan"

199 Publications

Mouse Model of Loeys-Dietz Syndrome Shows Elevated Susceptibility to Periodontitis via Alterations in Transforming Growth Factor-Beta Signaling.

Front Physiol 2021 11;12:715687. Epub 2021 Aug 11.

Department of Periodontology, Osaka University Graduate School of Dentistry, Suita, Japan.

Loeys-Dietz syndrome (LDS) is a syndromic connective tissue disorder caused by a heterozygous missense mutation in genes that encode transforming growth factor (TGF)-β receptor () and . We encountered a patient with LDS, who had severe periodontal tissue destruction indicative of aggressive periodontitis. The patient had a missense mutation in the glycine and serine-rich domain of exon 3. This G-to-T mutation at base 563 converted glycine to valine. We established an LDS model knock-in mouse that recapitulated the LDS phenotype. Homozygosity of the mutation caused embryonic lethality and heterozygous knock-in mice showed distorted and ruptured elastic fibers in the aorta at 24 weeks of age and died earlier than wildtype (WT) mice. We stimulated mouse embryonic fibroblasts (MEFs) from the knock-in mouse with TGF-β and examined their responses. The knock-in MEFs showed downregulated mRNA expression and phosphorylation of Smad2 to TGF-β compared with WT MEFs. To clarify the influence of TGF-β signaling abnormalities on the pathogenesis or progression of periodontitis, we performed pathomolecular analysis of the knock-in mouse. There were no structural differences in periodontal tissues between WT and LDS model mice at 6 or 24 weeks of age. Micro-computed tomography revealed no significant difference in alveolar bone resorption between WT and knock-in mice at 6 or 24 weeks of age. However, TGF-β-related gene expression was increased significantly in periodontal tissues of the knock-in mouse compared with WT mice. Next, we assessed a mouse periodontitis model in which periodontal bone loss was induced by oral inoculation with the bacterial strain W83. After inoculation, we collected alveolar bone and carried out morphometric analysis. -induced alveolar bone loss was significantly greater in LDS model mice than in WT mice. Peritoneal macrophages isolated from mice showed upregulation of inflammatory cytokine mRNA expression induced by lipopolysaccharide compared with WT macrophages. In this study, we established an LDS mouse model and demonstrated that LDS model mice had elevated susceptibility to -induced periodontitis, probably through TGF-β signal dysfunction. This suggests that TGF-β signaling abnormalities accelerate the pathogenesis or progression of periodontitis.
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http://dx.doi.org/10.3389/fphys.2021.715687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385443PMC
August 2021

PPARγ-Induced Global H3K27 Acetylation Maintains Osteo/Cementogenic Abilities of Periodontal Ligament Fibroblasts.

Int J Mol Sci 2021 Aug 11;22(16). Epub 2021 Aug 11.

Department of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.

The periodontal ligament is a soft connective tissue embedded between the alveolar bone and cementum, the surface hard tissue of teeth. Periodontal ligament fibroblasts (PDLF) actively express osteo/cementogenic genes, which contribute to periodontal tissue homeostasis. However, the key factors maintaining the osteo/cementogenic abilities of PDLF remain unclear. We herein demonstrated that PPARγ was expressed by in vivo periodontal ligament tissue and its distribution pattern correlated with alkaline phosphate enzyme activity. The knockdown of PPARγ markedly reduced the osteo/cementogenic abilities of PDLF in vitro, whereas PPARγ agonists exerted the opposite effects. PPARγ was required to maintain the acetylation status of H3K9 and H3K27, active chromatin markers, and the supplementation of acetyl-CoA, a donor of histone acetylation, restored PPARγ knockdown-induced decreases in the osteo/cementogenic abilities of PDLF. An RNA-seq/ChIP-seq combined analysis identified four osteogenic transcripts, RUNX2, SULF2, RCAN2, and RGMA, in the PPARγ-dependent active chromatin region marked by H3K27ac. Furthermore, RUNX2-binding sites were selectively enriched in the PPARγ-dependent active chromatin region. Collectively, these results identified PPARγ as the key transcriptional factor maintaining the osteo/cementogenic abilities of PDLF and revealed that global H3K27ac modifications play a role in the comprehensive osteo/cementogenic transcriptional alterations mediated by PPARγ.
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http://dx.doi.org/10.3390/ijms22168646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395443PMC
August 2021

Sheathed in-situ room-temperature growth covalent organic framework solid-phase microextraction fiber for detecting ultratrace polybrominated diphenyl ethers from environmental samples.

Anal Chim Acta 2021 Sep 16;1176:338772. Epub 2021 Jun 16.

Center of Advanced Analysis and Gene Sequencing, Zhengzhou University, Kexue Avenue 100, Zhengzhou, Henan, 450001, PR China; KLGHEI of Environment and Energy Chemistry, School of Chemistry, Sun Yat- Sen University, Guangzhou, Guangdong, 510275, PR China. Electronic address:

The extraction performance of solid-phase microextraction (SPME) fiber is significantly influenced by coating materials and fabricating process. It is urgently needed for fabricating robust SPME fiber with facile preparation methods. Herein, a novel polyimide (PI) @ covalent organic framework (COF) synthesized by 1,3,5-Tris (4-aminophenyl) benzene (TPB) and 2,5-dimethoxyterephthalaldehyde (DMTP) fiber, named [email protected] fiber, was successfully fabricated with facile method at room temperature. Firstly, a COF crystals TPB-DMTP was in situ grown on stainless steel fiber, where the COF crystals was synthesized by the Schiff-base reaction between TPB and DMTP. Subsequently, the COF coating was covered with an ultrathin layer of PI through a simple dip-coating method to improve the fiber stability. By coupled [email protected] SPME fiber with gas chromatography-negative chemical ion-mass spectrometry (GC-NCI-MS), a sensitive analytical method was established for the determination of ultratrace polybrominated diphenyl ethers (PBDEs) in water sample. To achieve the best efficiency and sensitivity for the analysis of PBDEs, six potential influencing factors in extraction step and desorption step were optimized. Under optimized conditions, the established method showed high enhancement factors of 1470-3555, wide linear range of 0.05-100 ng L, low detection limits of 0.0083-0.0190 ng L, good repeatability for intra-day in the range of 3.71%-7.62% and inter-day in the range of 5.12%-8.81%, good reproducibility in the range of 6.83%-9.21%. The satisfactory recovery was ranged from 79.2% to 117.3% in determining real water samples. The excellent experimental performance was mainly attributed to the large specific surface area of TPB-DMTP, as well as the high permeability of porous PI film. The results demonstrated that the COF-based fiber showed great potential for analysis of PBDEs in complex environmental samples.
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http://dx.doi.org/10.1016/j.aca.2021.338772DOI Listing
September 2021

Preliminary investigation of microplastics in the production process of sea salt sourced from the Bohai Sea, China, using an optimised and consistent approach.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 Aug 9:1-14. Epub 2021 Aug 9.

School of Light Industry, Beijing Technology and Business University, Beijing, China.

In this study, we present a preliminary investigation of the characteristic changes in microplastic during the production process of sea salt in China. Five representative samples, including seawater, saturated brine, crude salt, coarse salt, and refined salt were successively collected from the production process of sea salt sourced from the Bohai Sea in China. The pretreatment method of microplastics was optimised and evaluated by the recovery rates of particle references. Microplastic characteristics in the five samples were investigated using consistent pretreatment, microscopic, and spectroscopic analyses. Results indicated that microplastics were present in every stage of sea salt production. During the "journey" of microplastics in salt production from seawater-to-seawater evaporation, to salt crystallisation, and external force processing, the abundance of microplastics with size ranging from 18 μm to 4936 μm increased substantially from 23 ± 8 to 413 ± 61 particles kg, while the proportion of larger microplastics (≥1000 μm) decreased from 33% to 9%. The results illustrate the effect of the production process on microplastic contamination of sea salt.
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http://dx.doi.org/10.1080/19440049.2021.1956691DOI Listing
August 2021

Thyroid Nodule Classification in Ultrasound Images by Fusion of Conventional Features and Res-GAN Deep Features.

Authors:
Yuan Hang

J Healthc Eng 2021 22;2021:9917538. Epub 2021 Jul 22.

School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, China.

In spite of the gargantuan number of patients affected by the thyroid nodule, the detection at an early stage is still a challenging task. Thyroid ultrasonography (US) is a noninvasive, inexpensive procedure widely used to detect and evaluate the thyroid nodules. The ultrasonography method for image classification is a computer-aided diagnostic technology based on image features. In this paper, we illustrate a method which involves the combination of the deep features with the conventional features together to form a hybrid feature space. Several image enhancement techniques, such as histogram equalization, Laplacian operator, logarithm transform, and Gamma correction, are undertaken to improve the quality and characteristics of the image before feature extraction. Among these methods, applying histogram equalization not only improves the brightness and contrast of the image but also achieves the highest classification accuracy at 69.8%. We extract features such as histograms of oriented gradients, local binary pattern, SIFT, and SURF and combine them with deep features of residual generative adversarial network. We compare the ResNet18, a residual convolutional neural network with 18 layers, with the Res-GAN, a residual generative adversarial network. The experimental result shows that Res-GAN outperforms the former model. Besides, we fuse SURF with deep features with a random forest model as a classifier, which achieves 95% accuracy.
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http://dx.doi.org/10.1155/2021/9917538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321711PMC
July 2021

Quincke Oscillations of Colloids at Planar Electrodes.

Phys Rev Lett 2021 Jun;126(25):258001

Department of Chemical Engineering, Columbia University, New York, New York 10027, USA.

Dielectric particles in weakly conducting fluids rotate spontaneously when subject to strong electric fields. Such Quincke rotation near a plane electrode leads to particle translation that enables physical models of active matter. In this Letter, we show that Quincke rollers can also exhibit oscillatory dynamics, whereby particles move back and forth about a fixed location. We explain how oscillations arise for micron-scale particles commensurate with the thickness of a field-induced boundary layer in the nonpolar electrolyte. This work enables the design of colloidal oscillators.
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http://dx.doi.org/10.1103/PhysRevLett.126.258001DOI Listing
June 2021

Covalent Triazine Framework Sorbent for Solid Phase Extraction of Fipronil and its Metabolite in Eggs with Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 Sep 22;38(9):1495-1505. Epub 2021 Jun 22.

College of Chemistry, Zhengzhou University, Zhengzhou, P. R. China.

A solid-phase extraction (SPE) method was established for fipronil and its metabolite residues (fipronil desulfinyl, fipronil sulphone and fipronil sulphide) in eggs with a covalent triazine framework (CTF) porous material as the adsorbent followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) detection. Multiple probes and quantum chemistry theory calculations were conducted to describe the versatile adsorption property directly and quantifiably. The conjugated structure of CTF and N-containing triazine generated π-π interactions and hydrogen bonds between the CTF and the targets, which led to high extraction efficiency and recoveries. The solid-phase extraction parameters, including amount of the adsorbent, type of eluent, amount of eluent and loading rate were investigated. Under the optimal experimental conditions, the recoveries of the analytes were between 85.5% and 103.2%, and the RSD (n = 5) was between 1.8% and 3.6%. The LODs and LOQs were 0.13-0.2 ng g and 0.5-0.8 ng g, respectively. The sorbent can effectively reduce the interference of the matrix and meet the detection requirements of fipronil and its metabolites in eggs. These results imply that the CTF as adsorbents have great potential in the analysis of trace targets in samples with complex matrices.
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http://dx.doi.org/10.1080/19440049.2021.1934573DOI Listing
September 2021

The feasibility and technical strategy of a fascia space priority approach in laparoscopic lateral lymph node dissection for advanced middle and low rectal cancer: a retrospective multicentre study.

Wideochir Inne Tech Maloinwazyjne 2021 Jun 9;16(2):312-320. Epub 2021 Apr 9.

Department of Colorectal Surgery, Tianjin Union Medical Centre, Tianjin, China.

Introduction: Laparoscopic lateral lymph node dissection (LLND) is an important treatment for patients with lateral lymph node metastasis.

Aim: To assess the technical feasibility and investigate the surgical outcomes after LLND using the fascia space priority approach for patients with advanced middle and low rectal cancer.

Material And Methods: Consecutive patients undergoing laparoscopic LLND using the fascia space priority approach from June 2017 to June 2020 were identified from 12 medical centres in mainland China. Three anatomic fascia spaces were dissected to establish the boundaries of the LLND, and the obturator and internal iliac lymph nodes were excised in an en bloc manner. Retrospective clinical data including patient characteristics, surgical details, and pathology were analysed.

Results: A total of 112 patients were identified. All surgeries were completed laparoscopically with no conversions. The mean operation time was 343.6 ±103.8 min for the entire procedure. The median blood loss was 100 ml (range: 100-700 ml). The median lymph node yield was 6 (range: 1-41), and lymph nodes were positive in 39.3% (44/112) of the patients. Sixteen (14.3%) patients had Clavien-Dindo I-II complications, no Clavien-Dindo III-IV complications were identified. The incidence of complications between the bilateral dissection group and the unilateral dissection group was not statistically different (p = 0.19). The complication rate between the "nCRT" group and the "no nCRT" group was not significantly different (p = 0.62) either. There were no perioperative deaths.

Conclusions: Laparoscopic LLND using the fascia space priority approach is feasible and safe for patients with lateral lymph node metastasis.
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http://dx.doi.org/10.5114/wiitm.2021.105143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193747PMC
June 2021

Afterglow Amplification for Fast and Sensitive Detection of Porphyria in Whole Blood.

ACS Appl Mater Interfaces 2021 Jun 10;13(24):27991-27998. Epub 2021 Jun 10.

Department of Chemistry & Institutes of Biomedical Sciences & State Key Laboratory of Molecular Engineering of Polymers, Fudan University, 220 Handan Road, Shanghai 200433, P. R. China.

Porphyria is a group of genetic photodermatoses that cause too much porphyrin to accumulate in the blood, skin, and liver, resulting in skin photosensitivity and damage, liver disease, or potential liver failure. Conventional detection methods include high-performance liquid chromatography and fluorescence spectrometry. However, these methods usually require complicated pretreatment and time-consuming processes. Therefore, efficient and fast detection of porphyria is urgently needed. Herein, we develop a molecular afterglow reporter-based sensing scheme for the detection of porphyrins in whole blood. The afterglow reporter can respond to the production of singlet oxygen (O) of porphyrins after light excitation, and the detection signals can be amplified through adjusting the amount of singlet oxygen and afterglow reporter molecules. Moreover, without the use of a real-time excitation source, afterglow signals can avoid the scattering and autofluorescence interference in biological samples, thereby reducing background noise. More importantly, we prove the applicability of the afterglow reporter in the quantitative detection of porphyrins in whole blood and demonstrate its great clinical potential.
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http://dx.doi.org/10.1021/acsami.1c08518DOI Listing
June 2021

Three new sesquiterpene polyol esters from .

J Asian Nat Prod Res 2021 Jun 7:1-8. Epub 2021 Jun 7.

High & New Technology Research Center of Henan Academy of Science, Zhengzhou 450002 PR China.

Three new sesquiterpene polyol ester compounds angulatins S-U, together with three known compounds were isolated from Maxim. According to mainly 1D NMR and 2D NMR analysis, the structures of the new compounds were completely determined as angulatin S (1β-furoyloxy-2β,8α-diisobutanoyloxy-9β-benzoyloxy-15-acetoxy-4α,6α-dihydroxy-β-dihydroagarofuran), angulatin T (1β,2β,6α-triacetoxy-8β,15-diisobutanoyloxy-9α-benzoyloxy-β-dihydroagrofuran), and angulatin U (1β,6α,15-triacetoxy-8β-isobutanoyloxy-9α-benzoyloxy-β-dihydroagarofuran).
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http://dx.doi.org/10.1080/10286020.2021.1931140DOI Listing
June 2021

Quantitative analysis and optimization design of the segmented planar integrated optical imaging system based on an inhomogeneous multistage sampling lens array.

Opt Express 2021 Apr;29(8):11869-11884

The non-uniformity of the lens array in the segmented planar integrated optical imaging system has not been studied. We design an inhomogeneous multistage sampling lens array based on a hierarchical multistage sampling lens array of the segmented planar integrated optical imaging system by changing the radius of the lenslet for frequency-selective sampling to optimize the design of the UV spatial frequency distribution. The signal transfer model of the proposed imaging system was established considering the radius of the lenslet and key waveguide parameters. Simulation results show that the proposed system produces better imaging quality owing to the selective sampling of frequency. Compared with the homogeneous multistage sampling lens array (radius of 1.8 mm), the optimal radius parameters of the inhomogeneous multistage sampling lens array are given for the same longest lens array length. Detailed calculations of the parameters of the waveguide connected to the lens array were performed. Our results provide a theoretical basis for the optimal design and process of a new segmented planar integrated optical imaging system.
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http://dx.doi.org/10.1364/OE.421298DOI Listing
April 2021

LC-MS/MS-based non-isotopically paired labeling (NIPL) strategy for the qualification and quantification of monosaccharides.

Talanta 2021 Aug 26;231:122336. Epub 2021 Mar 26.

College of Chemistry, Zhengzhou University, China.

Investigation into monosaccharides is critical for studies of oligosaccharides structure and function in biological processes. However, monosaccharides quantification is still challenge due to their isomeric structure and high hydrophilic properties. Besides, it was difficult to obtain isotopic internal standards (IS) of each monosaccharide in complex matrixes. Herein, we developed a novel strategy for the qualification and quantification of monosaccharides in urine using two structure analogs 1-(4-methylphenyl)-3-methyl-5-pyrazolone (MPMP) and1-phenyl-3-methyl-5-pyrazolone (PMP) as non-isotopically paired labeling (NIPL) reagents by liquid chromatograph-tandem mass spectrometry (LC-MS/MS). The derivatized monosaccharides by NIPL method not only had sufficient retention time differences on reversed-phase column, but also exhibited predominant product ion pairs (m/z 189 & m/z 175) in the multiple reaction monitoring (MRM) mode. In this method, PMP labeled standards were adopted as one-to-one internal standards (ISs). 12 urinary monosaccharides were successfully determined and the linear ranges expanded five orders of magnitude with limit of quantification (LOQ) varied from 0.09 ng mL to 0.36 ng mL as well as the accuracy higher than 98.15% and the relative standard derivation (RSD) lower than 7.92%. With assistance of multivariate analysis, the targeted monosaccharide biomarkers were firstly obtained for the diagnosis of bladder cancer. By the inexpensive NIPL reagents-MPMP/PMP, the developed strategy possessed the specific advantages of low cost, simple operation, high sensitivity and high accuracy for the qualification and quantitation of monosaccharides. As expected, this method will provide an alternative application potential for targeted metabolomics analysis.
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http://dx.doi.org/10.1016/j.talanta.2021.122336DOI Listing
August 2021

Design, synthesis, and biological evaluation of novel dual inhibitors targeting lysine specific demethylase 1 (LSD1) and histone deacetylases (HDAC) for treatment of gastric cancer.

Eur J Med Chem 2021 Aug 25;220:113453. Epub 2021 Apr 25.

School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, China. Electronic address:

LSD1 and HDAC are physical and functional related to each other in various human cancers and simultaneous pharmacological inhibition of LSD1 and HDAC exerts synergistic anti-cancer effects. In this work, a series of novel LSD1/HDAC bifunctional inhibitors with a styrylpyridine skeleton were designed and synthesized based on our previously reported LSD1 inhibitors. The representative compounds 5d and 5m showed potent activity against LSD1 and HDAC at both molecular and cellular level and displayed high selectivity against MAO-A/B. Moreover, compounds 5d and 5m demonstrated potent antiproliferative activities against MGC-803 and HCT-116 cancer cell lines. Notably, compound 5m showed superior in vitro anticancer potency against a panel of gastric cancer cell lines than ORY-1001 and SP-2509 with IC values ranging from 0.23 to 1.56 μM. Compounds 5d and 5m significantly modulated the expression of Bcl-2, Bax, Vimentin, ZO-1 and E-cadherin, induced apoptosis, reduced colony formation and suppressed migration in MGC-803 cancer cells. In addition, preliminary absorption, distribution, metabolism, excretion (ADME) studies revealed that compounds 5d and 5m showed acceptable metabolic stability in human liver microsomes with minimal inhibition of cytochrome P450s (CYPs). Those results indicated that compound 5m could be a promising lead compound for further development as a therapeutic agent in gastric cancers via LSD1 and HDAC dual inhibition.
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http://dx.doi.org/10.1016/j.ejmech.2021.113453DOI Listing
August 2021

Paraneoplastic immunoglobulin A nephropathy in a patient with lung adenocarcinoma: A case report and literature review.

J Int Med Res 2021 Apr;49(4):300060521996868

Department of Nephrology, The First Hospital of Jilin University, Changchun, Jilin Province, China.

Immunoglobulin A nephropathy (lgAN) is a common primary glomerulonephritis, but paraneoplastic IgAN has been rarely reported. This current case report describes a 49-year-old male patient that was referred with proteinuria, oedema and hypoproteinaemia after lung cancer surgery and before the first cycle of chemotherapy. Renal biopsy confirmed lgAN. The patient received four cycles of chemotherapy (first cycle: pemetrexed + nedaplatin; second to fourth cycle: pemetrexed + carboplatin). The symptoms of IgAN were gradually relieved with additional cycles of chemotherapy. At the latest follow-up on 10 February 2020, there was no evidence of lung cancer recurrence and all symptoms of lgAN had disappeared. lgAN combined with lung adenocarcinoma is quite rare, which suggests that IgAN might be a paraneoplastic manifestation of lung adenocarcinoma.
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http://dx.doi.org/10.1177/0300060521996868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113932PMC
April 2021

Gastroesophageal junction cancer with hepatic metastasis: Effective Treatment using microsphere embolization combined with transarterial infusion chemotherapy.

Dig Liver Dis 2021 Apr 22. Epub 2021 Apr 22.

Department of Minimal-Invasive Intervention, The Affiliated Cancer Hospital of Zhengzhou University, 127 Dongming Road, Zhengzhou, Henan 450003, China.

Purpose: To determine the safety and efficacy of microsphere embolization plus transarterial infusion chemotherapy for the treatment of gastroesophageal junction cancer with hepatic metastasis.

Methods: Sixty patients with gastroesophageal junction cancer and hepatic metastasis were randomly divided into two groups: group A (treatment group), which was treated with transarterial infusion chemotherapy plus microsphere embolization for gastroesophageal cancer, and with transarterial chemoembolization for hepatic metastasis; and group B (control group), which was treated with transarterial infusion chemotherapy for gastroesophageal cancer, and with transarterial chemoembolization for hepatic metastasis. The chemotherapy regimen used consisted of oxaliplatin plus FUDR. The embolization agent used for gastroesophageal cancer and the hepatic metastasis were Embosphere and ultra-liquefied lipiodol, respectively.

Results: The median survival time of patients in group A was 19 months, with survival rates at 12, 18, and 24 months of 93.3%, 60.0%, and 23.3%, respectively. The median survival time of patients in group B was 13 months, with survival rates at 12, 18, and 24 months of 60.0%, 30.0%, and 3.3%, respectively. There was a significant difference in survival between the two groups (P = 0.00). One month after treatment, the severity of dysphagia was significantly less in group A, as compared to that in group B (p < 0.001).

Conclusion: Treatment of gastroesophageal junction cancer with hepatic metastasis by transarterial infusion chemotherapy plus microsphere embolization can rapidly reduce tumor size near the gastroesophageal junction. This treatment is an effective therapeutic option for these patients as it can relieve dysphagia and improve long-term survival rate.
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http://dx.doi.org/10.1016/j.dld.2021.03.023DOI Listing
April 2021

Effect of HO Molecule Adsorption on the Electronic Structure and Optical Properties of the CsI(Na) Crystal.

Materials (Basel) 2021 Mar 31;14(7). Epub 2021 Mar 31.

Radiation Detection Research Center, Northwest Institute of Nuclear Technology, Xi'an 710024, China.

We investigated HO molecule adsorption that had an effect on the luminescence properties of the CsI(Na) crystal using experiments and first-principle calculations. We measured the emission spectra of the CsI(Na) crystal at different exposure times under gamma ray excitation. The experimental results showed that the energy resolution of the CsI(Na) crystal was worse when the crystal surface adsorbed more HO molecules, and the crystal surface deliquescence decreased the luminescence efficiency of the CsI(Na) crystal. We studied the band structure, density of states, and optical properties changes caused by HO molecule adsorption on the CsI(Na) (010) surface. The generalized gradient approximation (GGA) was used to describe the exchange and correlation potential between the electrons. Our calculation results showed that the band gap width of the CsI(Na) (010) surface decreased after adsorbing HO molecules, while three new peaks appeared in the valence band, and the absorption coefficient decreased from 90,000 cm to 65,000 cm, and the reflection coefficient decreased from 0.195 to 0.105. Further, the absorption coefficient was reduced by at least 25% because of HO molecule adsorption, which led to the luminescence degradation of the CsI(Na) crystal.
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http://dx.doi.org/10.3390/ma14071720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037489PMC
March 2021

Canine Papillomavirus 2 E6 Does Not Interfere With UVB-Induced Upregulation of p53 and p53-Regulated Genes.

Front Vet Sci 2021 3;8:570982. Epub 2021 Mar 3.

Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.

Cutaneous papillomaviruses are oncogenic viruses that cause severe, persistent infections that can develop into skin cancers within ultraviolet (UV)-exposed skin of immunodeficient individuals, such as those with X-linked severe combined immunodeficiency (XSCID). A canine research model of XSCID exhibits a similar phenotype; these dogs develop severe canine papillomavirus 2 (CPV2) infections that often progress to cancer. Thus, the dog is a natural, spontaneous model to investigate cutaneous papillomavirus infections in immunodeficient patients. The human papillomavirus oncogene E6 contributes to cancer development, in part, by initiating degradation of the tumor suppressor protein p53, or by inhibiting upregulation of p53-dependent genes required within the cell growth arrest and apoptotic pathways, thereby leading to an accumulation of DNA damage required for oncogenesis. Currently, little is known about CPV2, and how it promotes cancer development. The aim of this study was to determine if CPV2 oncogene E6 similarly affects p53 upon activation by UV radiation, as well as the downstream p53-regulated genes necessary to control growth arrest and apoptosis. We determined that cutaneous CPV2 E6 does not degrade p53, or interfere with the upregulation of p53-regulated genes p21, Bax, Bak, or lncRNA-p21, suggesting that CPV2 may use a p53-independent mechanism to contribute to oncogenesis.
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http://dx.doi.org/10.3389/fvets.2021.570982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7965962PMC
March 2021

Downregulation of lncRNA RPLP0P2 inhibits cell proliferation, invasion and migration, and promotes apoptosis in colorectal cancer.

Mol Med Rep 2021 05 2;23(5). Epub 2021 Mar 2.

Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China.

Recent studies have revealed that long noncoding RNAs (lncRNAs) are closely associated with colorectal cancer (CRC); however, the role of the lncRNA RPLP0P2 in CRC remains largely unknown. In the present study, RNA expression profiles of CRC were collected from The Cancer Genome Atlas database and the prognosis of CRC with respect to RPLP0P2 was assessed. Subsequently, RPLP0P2 expression was knocked down in the human CRC cell line RKO using a short hairpin RNA (shRNA) lentivirus, and the biological behaviors of the cells, such as proliferation, migration, cell cycle progression and apoptosis, were examined. The results demonstrated that the expression levels of RPLP0P2 were higher in CRC tissue compared with those in normal tissue, and RPLP0P2 was associated with prognosis. RPLP0P2 knockdown significantly decreased cell colony formation, migration and invasion, and arrested CRC cells in the S phase to G2/M phase transition. Furthermore, apoptosis was significantly increased in CRC cells infected with the RPLP0P2 shRNA lentivirus compared with in the control group. In conclusion, RPLP0P2 may promote proliferation, invasion and migration, and inhibit apoptosis of CRC cells, suggesting that RPLP0P2 may function as an oncogene in CRC.
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http://dx.doi.org/10.3892/mmr.2021.11948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974314PMC
May 2021

Efficacy and safety of transcatheter arterial chemoembolization combined with either I seed implantation or apatinib in hepatocellular carcinoma with portal vein tumor thrombosis: A retrospective comparative study.

J Cancer Res Ther 2020 ;16(7):1691-1697

Minimally Invasive and Interventional Department, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, China.

Aims: The aims of the study were to compare the efficacy and safety between transcatheter arterial chemoembolization (TACE) combined with I seed implantation (TACE-I) or with apatinib (TACE-Apatinib) in HCC-portal vein tumor thrombosis (PVTT) patients.

Setting And Design: We retrospectively evaluated the medical records of consecutive patients with HCC-PVTT who had undergone treatment with either TACE-I or TACE-Apatinib between January 2018 and June 2019.

Materials And Methods: The response was assessed at the last follow-up, and the outcomes were compared between the two groups. Progression-free survival (PFS), overall survival (OS), and treatment-related complications were evaluated. Statistical analysis used the 2-sample Student's t-test and Fisher's exact test.

Results: This study enrolled 48 patients; 21 were treated with TACE-Apatinib and 27 with TACE-I. For PVTT, the disease control rate (DCR) was 23.81% in the TACE-Apatinib group and 77.78% in the TACE-I group. The objective response rate (ORR) in the TACE-Apatinib group was remarkably lower. The DCR of intrahepatic lesions was 85.71% in the TACE-Apatinib group and 81.48% in the TACE-I group. There was no statistically significant difference in the ORR of intrahepatic lesions. Median OS was significantly longer in the TACE-I group (13.3 vs. 10.8 months). Similarly, the median PFS was significantly longer in the TACE-I group (9.7. vs. 6.6 months). Multivariate and univariate analyses showed that TACE-I was an independent prognostic factor for OS.

Conclusions: Compared with TACE-Apatinib, TACE-I seed implantation can effectively prolong PVTT progression, PFS, and OS in HCC patients with PVTT.
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http://dx.doi.org/10.4103/jcrt.JCRT_1587_20DOI Listing
January 2020

Geminate labels programmed by two-tone microdroplets combining structural and fluorescent color.

Nat Commun 2021 01 29;12(1):699. Epub 2021 Jan 29.

Department of Materials Science and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, 200433, P. R. China.

Creating a security label that carries entirely distinct information in reflective and fluorescent states would enhance anti-counterfeiting levels to deter counterfeits ranging from currencies to pharmaceuticals, but has proven extremely challenging. Efforts to tune the reflection color of luminescent materials by modifying inherent chemical structures remain outweighed by substantial trade-offs in fluorescence properties, and vice versa, which destroys the information integrity of labels in either reflection or fluorescent color. Here, a strategy is reported to design geminate labels by programming fluorescent cholesteric liquid crystal microdroplets (two-tone inks), where the luminescent material is 'coated' with the structural color from helical superstructures. These structurally defined microdroplets fabricated by a capillary microfluidic technique contribute to different but intact messages of both reflective and fluorescent patterns in the geminate labels. Such two-tone inks have enormous potential to provide a platform for encryption and protection of valuable authentic information in anti-counterfeiting technology.
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http://dx.doi.org/10.1038/s41467-021-20908-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846849PMC
January 2021

DMP-1 promoter-associated antisense strand non-coding RNA, panRNA-DMP-1, physically associates with EGFR to repress EGF-induced squamous cell carcinoma migration.

Mol Cell Biochem 2021 Apr 9;476(4):1673-1690. Epub 2021 Jan 9.

Department of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.

Accumulating evidence suggests that specific non-coding RNAs exist in many types of malignant tissues, and are involved in cancer invasion and metastasis. However, little is known about the precise roles of non-coding RNAs in squamous cell carcinoma (SQCC) invasion and migration. Recently, the dentin matrix protein-1 (DMP-1) gene locus was identified as a transcriptionally active site in squamous cell carcinoma (SQCC) tissue and cells. However, it is unclear whether RNA associated with cell migration exist at the DMP-1 gene locus in SQCC cells. We identified a novel promoter-associated non-coding RNA in the antisense strand of DMP-1 gene locus, promoter-associated non-coding RNA (panRNA)-DMP-1, by the RACE method in SQCC cells and tissues, and characterized the functions of panRNA-DMP-1 in EGF-driven SQCC cell migration. The inhibition of endogenous panRNA-DMP-1 expression by specific siRNAs and exogenous over-expression of panRNA-DMP-1 resulted in increased and suppressed cellular migration toward EGF in SQCC cells, respectively, and nuclear expression of panRNA-DMP-1 was induced by EGF stimulation. Mechanistically, suppression of panRNA-DMP-1 expression increased EGFR nuclear localization upon EGF treatment and nuclear panRNA-DMP-1 physically interacted with EGFR, which was confirmed by RNA immunoprecipitation assay using a bacteriophage-delivered PP7 RNA labeling system. Furthermore, co-immunoprecipitation assay revealed that suppression of panRNA-DMP-1 stabilized EGFR interaction with STAT3, a known co-transcription factors of EGFR, to induce migratory properties in many cancer cells. Based on these findings, panRNA-DMP-1 is an EGFR-associating RNA that inhibits the EGF-induced migratory properties of SQCC possibly by regulating EGFR nuclear localization and EGFR binding to STAT3.
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http://dx.doi.org/10.1007/s11010-020-04046-5DOI Listing
April 2021

Copy Number Analysis Reveal Genetic Risks of Penile Cancer.

Front Oncol 2020 14;10:596261. Epub 2020 Dec 14.

Urology Department, The Affiliated Hospital of Qingdao University, Qingdao, China.

Objectives: To evaluate copy number alterations (CNAs) in genes associated with penile cancer (PeC) and determine their correlation and prognostic ability with PeC.

Methods: Whole-exome sequencing was performed for tumor tissue and matched normal DNA of 35 patients diagnosed with penile squamous cell carcinoma from 2011 to 2016. Somatic CNAs were detected using the Genome Analysis Toolkit (GATK). Retrospective clinical data were collected and analyzed. All the data were statistically analyzed using SPSS 16.0 software. The cancer-specific survival rates were estimated by Kaplan-Meier curves and compared with the log-rank test.

Results: CNAs in the gene was detected in 19 (amplification: 54.29%) patients. Other CNAs gene targets were (amplification: 45.72%, deletion: 8.57%), (amplification: 2.86%, deletion: 51.43%), (amplification: 34.29%, deletion: 2.86%), (amplification: 5.71%, deletion: 42.86%), (amplification: 14.29%, deletion: 20.00%), (amplification: 17.14%, deletion: 2.86%), (amplification:14.29%, deletion: 48.57%), and (amplification: 5.71%, deletion: 45.72%). The CNAs in and correlated significantly with patient prognosis (P<0.05). The 3-year Recurrence-free survival rate was 87.10% among patients followed up. The 5-year survival rate of patients with amplification was 69.2%, compared to 94.4% in the non-amplification group. The 5-year survival rate of patients with amplification was 65.6%, compared to 94.7% in the non-amplification group. The PPI network showed that and might play meaningful functional roles in PeC.

Conclusion: and amplification and deletion were apparent in PeC. and were hub genes in PeC. and amplification was also detected and analyzed, and the findings indicated that these two genes are predictors of poor prognosis in PeC.
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http://dx.doi.org/10.3389/fonc.2020.596261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768990PMC
December 2020

GSK3β Plays a Negative Role During White Spot Syndrome Virus (WSSV) Infection by Regulating NF-κB Activity in Shrimp .

Front Immunol 2020 26;11:607543. Epub 2020 Nov 26.

College of Fisheries, Guangdong Ocean University, Zhanjiang, China.

Glycogen synthase kinase-3 (GSK3), a cytoplasmic serine/threonine-protein kinase involved in a large number of key cellular processes, is a little-known signaling molecule in virus study. In this study, a GSK3 protein which was highly similar to GSK3β homologs from other species in (designated as LvGSK3β) was obtained. LvGSK3β was expressed constitutively in the healthy , at the highest level in the intestine and the lowest level in the eyestalk. White spot syndrome virus (WSSV) reduced LvGSK3β expression was in immune tissues including the hemocyte, intestine, gill and hepatopancreas. The inhibition of LvGSK3β resulted in significantly higher survival rates of during WSSV infection than the control group, and significantly lower WSSV viral loads in LvGSK3β-inhibited were observed. Knockdown of LvGSK3β by RNAi resulted in increases in the expression of LvDorsal and several NF-κB driven antimicrobial peptide (AMP) genes (including ALF, PEN and crustin), but a decrease in LvCactus expression. Accordingly, overexpression of LvGSK3β could reduce the promoter activity of LvDorsal and several AMPs, while the promoter activity of LvCactus was increased. Electrophoretic mobility shift assays (EMSA) showed that LvDorsal could bind to the promoter of LvGSK3β. The interaction between LvGSK3β and LvDorsal or LvCactus was confirmed using co-immunoprecipitation (Co-IP) assays. In addition, the expression of LvGSK3β was dramatically reduced by knockdown of LvDorsal. In summary, the results presented in this study indicated that LvGSK3β had a negative effect on by mediating a feedback regulation of the NF-κB pathway when it is infected by WSSV.
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http://dx.doi.org/10.3389/fimmu.2020.607543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725904PMC
June 2021

Fast and programmable locomotion of hydrogel-metal hybrids under light and magnetic fields.

Sci Robot 2020 12;5(49)

Center for Bio-inspired Energy Science, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.

The design of soft matter in which internal fuels or an external energy input can generate locomotion and shape transformations observed in living organisms is a key challenge. Such materials could assist in productive functions that may range from robotics to smart management of chemical reactions and communication with cells. In this context, hydrated matter that can function in aqueous media would be of great interest. Here, we report the design of hydrogels containing a scaffold of high-aspect ratio ferromagnetic nanowires with nematic order dispersed in a polymer network that change shape in response to light and experience torques in rotating magnetic fields. The synergistic response enables fast walking motion of macroscopic objects in water on either flat or inclined surfaces and also guides delivery of cargo through rolling motion and light-driven shape changes. The theoretical description of the response to the external energy input allowed us to program specific trajectories of hydrogel objects that were verified experimentally.
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http://dx.doi.org/10.1126/scirobotics.abb9822DOI Listing
December 2020

A Network Pharmacology to Explore the Mechanism of Membranaceus in the Treatment of Diabetic Retinopathy.

Evid Based Complement Alternat Med 2020 2;2020:8878569. Epub 2020 Nov 2.

Surgical Department of Fundus Disease and Trauma, Eye Hospital, China Academy of Chinese Medical Sciences, Beijing 100040, China.

Background: Diabetic retinopathy (DR) includes a series of typical lesions affected by retinal microvascular damage caused by diabetes mellitus (DM), which not only seriously damages the vision, affecting the life's quality of patients, but also brings a considerable burden to the family and society. Membranaceus (AM) is a commonly used medicine in clinical therapy of eye disorders in traditional Chinese medicine (TCM). In recent years, it is also used for treating DR, but the specific mechanism is unclear. Therefore, this study explores the potential mechanism of AM in DR treatment by using network pharmacology.

Methods: Based on the oral bioavailability (OB) and drug likeness (DL) of two ADME (absorption, distribution, metabolism, excretion) parameters, Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), Swiss Target Prediction platform, GeneCards, and OMIM database were used to predict and screen the active compounds of AM, the core targets of AM in DR treatment. The Metascape data platform was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis on the core targets.

Results: 24 active compounds were obtained, such as quercetin, kaempferol, and astragaloside IV. There were 169 effective targets of AM in DR treatment, and the targets were further screened and finally, 38 core targets were obtained, such as VEGFA, AKT1, and IL-6. EGFR tyrosine kinase inhibitor resistance, AGE-RAGE signaling pathway in diabetic complications, PI3K-Akt signaling pathway, and other metabolic pathways participated in oxidative stress, cell apoptosis, angiogenesis signal transduction, inflammation, and other biological processes.

Conclusion: AM treats DR through multiple compounds, multiple targets, and multiple pathways. AM may play a role in the treatment of DR by targeting VEGFA, AKT1, and IL-6 and participating in oxidative stress, angiogenesis, and inflammation.
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http://dx.doi.org/10.1155/2020/8878569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652614PMC
November 2020

Sarcomatoid Carcinoma in the Head and Neck: A Population-Based Analysis of Outcome and Survival.

Laryngoscope 2021 02 21;131(2):E489-E499. Epub 2020 Jul 21.

Department of Radiation Oncology, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.

Objectives: To characterize sarcomatoid cell carcinoma (SaC) in head and neck, explore the value of radiotherapy (RT) and chemotherapy, and build a nomogram to predict the prognosis.

Study Design: Retrospective cohort study.

Methods: In total, 559 patients diagnosed with head and neck SaC from 2004 to 2015 were included from the Surveillance, Epidemiology, and End Results program. All the cases were divided into training (N = 313) and validation (N = 246) cohorts according to the year of diagnosis. The cases were analyzed on the age, site, sex, race, T stage, N stage, M stage, surgery, RT, and chemotherapy. Cancer-specific survival (CSS) and overall survival (OS) were compared among disease-related categories. The parameters significantly correlated with CSS were used to construct a nomogram.

Results: The multivariate analysis showed that age, T stage, N stage, and M stage were significantly correlated with CSS and OS. Overall, RT was correlated with improved CSS for Stage T3-4 and Stage N1-3. The subgroup analysis showed that RT was correlated with CSS in the Stage N1-3 patients after surgery while chemotherapy indicated an improved survival for Stage T3-4 and N1-3 patients without surgery. The prognostic nomogram was constructed and had a powerful discriminatory ability with the C-index of CSS: 0.711.

Conclusion: Late-stage head and neck SaC patients unfit for surgery need comprehensive treatment based on chemotherapy, and patients with node metastasis require adjuvant RT after surgery. Generally, RT might improve the survival of late-stage patients. A reliable and powerful nomogram was established that can provide an individual prediction of CSS for head and neck SaC.

Level Of Evidence: 3 Laryngoscope, 131:E489-E499, 2021.
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http://dx.doi.org/10.1002/lary.28956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818253PMC
February 2021

Coexistence of antineutrophil cytoplasmic antibody-associated vasculitis and membranous nephropathy: a case report.

Ann Palliat Med 2021 May 9;10(5):5875-5880. Epub 2020 Oct 9.

Department of Nephrology, First Hospital of Jilin University, Changchun, China.

Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) and membranous nephropathy (MN) are two types of diseases that can both involve kidneys. In recent years, there are some reports about the concurrence of these two diseases. However, the precise mechanism, clinical course, and optimal treatment of patients with this unusual combination of renal diseases are little known. Previously reported cases of MN combined with AAV presented characteristics of both MN and crescentic glomerulonephritis, manifesting rapid disease progression and poor prognosis. Diagnosis and treatment are essential and challenging. Here, we report a case of AAV and MN in a 62 years old man, who was admitted to hospital due to edema, low-grade fever, and cough. The patient showed a different pathological feature from previous cases, with no crescent formation. By analyzing the diagnoses and potential pathogenesis, we considered MN was secondary to the present AAV and suspected the existence of other alternative mechanisms. Also, we applied a new therapy, glucocorticoid with mizoribine. After one-year follow-up, this treatment is feasible, safe, and leads to a clinical improvement for AAV combined with MN. In this case, two diseases overlapped, and the laboratory analysis and pathology are both required to ensure diagnostic accuracy. The principal aim of this case report is to highlight the diagnostic challenge and distinct treatment in the simultaneous occurrence of AAV combined with MN.
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http://dx.doi.org/10.21037/apm-20-1323DOI Listing
May 2021

Traditional Chinese medicine Xiaosheng Powder for dry eye disease: A protocol for systematic review and meta analysis.

Medicine (Baltimore) 2020 Aug;99(35):e22019

Department of Ophthalmology, Eye Hospital.

Background: Dry eye disease (DED) has shown a significant increase in recent years, which seriously affects people's work and life. Xiaosheng Powder, a traditional Chinese medicine decoction, has been widely used in treating DED. However, there is no systematic review of the results of the study on this therapeutic effect. The purpose of this review is to evaluate the effectiveness and safety of Xiaosheng Powder in the treatment of DED.

Methods And Analysis: The electronic databases to be searched will include MEDLINE (PubMed), Cochrane Central Register of Controlled Trials in the Cochrane Library, Excerpta Medica Database, China National Knowledge Infrastructure, China Scientific Journal Database, Wanfang Database and Chinese Biomedical Literature Database. Papers in English or Chinese published from inception to 2020 will be included without any restrictions. Improvement in Ocular Surface Disease Index will be assessed as the primary outcomes. Tear break-up time, Schirmer I test, fluorescent, adverse events, and the recurrence rate after at least 3 months of the treatment will be evaluated as secondary outcomes. We will conduct a meta-analysis of randomized controlled trial if possible. The methodological qualities, including the risk of bias, will be evaluated using the Cochrane risk of bias assessment tool, while confidence in the cumulative evidence will be evaluated using the Grading of Recommendations Assessment, Development, and Evaluation approach.

Ethics And Dissemination: It is not necessary for a formal ethical approval because the data is not individualized. The results of this review will offer implications for the use of Xiaosheng Powder as a treatment for DED. This knowledge will inform recommendations by ophthalmologist and researchers who are interested in the treatment of DED. The findings of this systematic review will be disseminated through peer-reviewed publications and conference presentations.

Trail Registration Number: PROSPERO CRD42020147709.
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http://dx.doi.org/10.1097/MD.0000000000022019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7458267PMC
August 2020

Research on the Decoupling of Water Resources Utilization and Agricultural Economic Development in Gansu Province from the Perspective of Water Footprint.

Int J Environ Res Public Health 2020 08 9;17(16). Epub 2020 Aug 9.

School of Economics and Management, Southeast University, Nanjing 211189, China.

Objectively evaluating the decoupling status of water resources utilization and economic development is an important sign of judging the sustainability of regional economic development. From the perspective of water footprint (WF), this paper expands the scope of water resources accounting by assessing agricultural blue WF, green WF and gray WF. The Tapio decoupling index was used to explore the decoupling status of agricultural WF and economic development in Gansu Province from 2006 to 2015, and the logarithmic mean divisor index (LMDI) decomposition model was used to identify the main driving factors of agricultural WF changes and explore the degree of divergence between agricultural economic development and water resources utilization. The results showed that agricultural economic growth was a main factor for the increase of WF; the improvement of agricultural production technology had a restraining effect, and the population effect and structural effect had a lesser effect. During the research period, the relationship between agricultural WF and economic growth in Gansu Province changed from weak decoupling to strong decoupling, and the contributing factors to decoupling were in descending order: economic, technological, structural and population. Finally, this paper puts forward suggestions on optimizing planting structure, improving agricultural technology and economic development mode to promote the sustainable development of local agriculture.
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http://dx.doi.org/10.3390/ijerph17165758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460227PMC
August 2020

The phytochemical epigallocatechin gallate prolongs the lifespan by improving lipid metabolism, reducing inflammation and oxidative stress in high-fat diet-fed obese rats.

Aging Cell 2020 09 30;19(9):e13199. Epub 2020 Jul 30.

Department of Nutrition and Food Hygiene, College of Public Health, Harbin Medical University, Harbin, Heilongjiang, China.

We have recently reported that epigallocatechin gallate (EGCG) could extend lifespan in healthy rats. This study aimed to investigate the effects and mechanisms of a high dose of EGCG in extending the lifespan of obese rats. Ninety adult male Wistar rats were randomly divided into the control (NC), high-fat (HF) and EGCG groups. Serum glucose and lipids, inflammation and oxidative stress were dynamically determined from adulthood to death, and the transcriptome and proteome of the liver were also examined. The median lifespans of the NC, HF and EGCG groups were 693, 599 and 683 days, respectively, and EGCG delayed death by 84 days in obese rats. EGCG improved serum glucose and lipids and reduced inflammation and oxidative stress associated with aging in obese rats induced by a high-fat diet. EGCG also significantly decreased the levels of total free fatty acids (FFAs), SFAs and the n-6/n-3 ratio but significantly increased the n-3 FFAs related to longevity. The joint study of the transcriptome and proteome in liver found that EGCG exerted its effects mainly by regulating the suppression of hydrogen peroxide and oxygen species metabolism, suppression of oxidative stress, activation of fatty acid transport and oxidation and cholesterol metabolism. EGCG significantly increased the protein expression of FOXO1, Sirt1, CAT, FABP1, GSTA2, ACSL1 and CPT2 but significantly decreased NF-κB, ACC1 and FAS protein levels in the livers of rats. All the results indicate that EGCG extends lifespan by improving FFA metabolism and reducing the levels of inflammatory and oxidative stress in obese rats.
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http://dx.doi.org/10.1111/acel.13199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511879PMC
September 2020
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