Publications by authors named "Hang Liu"

422 Publications

Amino-functionalized MIL-88B as heterogeneous photo-Fenton catalysts for enhancing tris-(2-chloroisopropyl) phosphate (TCPP) degradation: Dual excitation pathways accelerate the conversion of Fe to Fe under visible light irradiation.

J Hazard Mater 2021 Nov 17:127782. Epub 2021 Nov 17.

Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.

In this work, the amino-functionalized metal-organic frameworks (MIL-88B-NH) was synthesized, characterized and used as heterogeneous photo-Fenton catalyst for tris-(2-chloroisopropyl) phosphate (TCPP) degradation. The photo-Fenton activity of MIL-88B-NH was investigated on the basis of influence factors, such as initial pH and TCPP concentration, and coexisting impurities. The results revealed that MIL-88B-NH+HO+Vis system exhibited a satisfactory degradation efficiency of TCPP (almost 100%) within 60 min accompanied by a good reusability. Noticeably, the degradation kinetics constant of TCPP by MIL-88B-NH+HO+Vis system was 0.086 min, which was visibly higher than that of MIL-88B+HO+Vis system (0.021 min) since the addition of amino-functionalized organic linker inhibiting the recombination rate of the photo-generated electron-hole pairs and improving the visible light response. Combined with the characterization, the conversion of Fe to Fe could be accelerated by the photo-generated electron from the excitation of Fe-O clusters and NH functionalities, which strengthened the decomposition of HO and formed plenty •OH. Simultaneously, six steady products were validated and potential degradation pathways of TCPP were proposed. It was anticipated that MIL-88B-NH could be considered as a desirable and alternative candidate in the application of heterogeneous photo-Fenton reaction to control the environmental risks caused by organophosphate flame retardants (OPFRs).
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http://dx.doi.org/10.1016/j.jhazmat.2021.127782DOI Listing
November 2021

Post-transplantation Fluorine-18 Fluorodeoxyglucose Positron Emission Tomography in Patients with Lymphoblastic Lymphoma is an Independent Prognostic Factor with an Impact on Progression-Free Survival but not Overall Survival.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211056478

Department of Nuclear Medicine, 74566the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

In the present study, we mainly aimed to evaluate the prognostic value of 2-deoxy-2-[F]fluoro-D-glucose ([F]F-FDG) positron emission tomography (PET)/computed tomography (CT) after allogeneic stem cell transplantation (allo-SCT) in lymphoblastic lymphoma (LBL) patients using Deauville Scores (DS). A total of 63 LBL patients who benefited from F-FDG PET-CT after allo-SCT in our institution between April 2010 and August 2020 were enrolled in this retrospective study. These above-mentioned patients were divided into two groups based on the Deauville criteria. Diagnostic efficiency of F-FDG PET/CT and integrated CT in detecting lymphoma were calculated. Consistencies were evaluated by comparing 18F-FDG PET/CT and integrated CT results through kappa coefficient. Kaplan-Meier method was used in survival analysis, and the log-rank method was adopted in comparisons. Prognostic factor analysis was performed by the Cox regression model. The sensitivity, specificity, positive predictive value, negative predictive value, accuracy of post-SCT F-FDG PET-CT were 100%(12/12), 92.2%(47/51), 75.0%(12/16), 100%(47/47) and 93.7%(59/63). The consistency of 18F-FDG PET-CT and integrated CT was moderate(Kappa = .702,P < .001). Positive post-SCT 18F-FDG PET-CT was associated with lower progression-free survival (PFS) but not overall survival (OS) (p = .000 and p = .056, respectively). The 3-year PFS of the PET-positive group and PET-negative group was 18.8% and 70.2%, respectively. Multivariate analysis showed that post-SCT PET-CT findings was an independent prognostic factor for PFS (p = .000; HR, 3.957; 95%CI, 1.839-8.514). Other factors independently affecting PFS were sex (p = .018; HR, 2.588; 95% CI, 1.181 - 5.670) and lactate dehydrogenase (LDH) (p = .005; HR, 3.246; 95% CI, 1.419 - 7.426). However, none of the above-mentioned factors were associated with OS. Collectively, we found that 18F-FDG PET-CT after allo-SCT was a strong indicator for PFS, but not OS, which might provide important evidence for the selection of subsequent treatment regimen for LBL patients. Trial registration number: ChiCTR2100046709.
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http://dx.doi.org/10.1177/15330338211056478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606727PMC
November 2021

Effectiveness of ozonated saline in the treatment of VX2 tumors in rabbits.

J Interv Med 2018 Aug 30;1(3):143-149. Epub 2019 Apr 30.

Interventional Department, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.

To investigate the efficacy, safety, and associated mechanisms of injected ozonated saline in the treatment of VX2 tumors. A total of 90 rabbits bearing VX2 tumors on their left hind legs were randomly divided into three groups. The control group (A) received normal saline, while groups B and C received 20 μg/mL and 40 μg/mL O/O ozonated saline, respectively. Rabbits were anesthetized and 2 mL of blood was drawn directly from the heart to measure serum concentrations of interleukin (IL-6) and tumor necrosis factor (TNF-α). The skin covering the VX2 tumor was cut in each rabbit and the maximum and vertical diameters of the tumors were measured under direct visualization. Several milliliters of saline, saline pre-treated with 20 μg/mL O/O, or saline pre-treated with 40 μg/mL O/O were directly injected into the tumors of groups A, B, and C, respectively (injection volume (milliliter) =1/2 volume of the tumor, V = 1/2ab). On days 4, 8 and 12 following treatment, 10 rabbits were randomly selected from each group for blood sample collection, and serum IL-6 and TNF-α were measured. The tumor growth rate was calculated by measuring the maximum and vertical diameters of the VX2 tumors under direct visualization. All selected rabbits were euthanized and the tumors, livers, and lungs were removed for pathological examination. The tumor necrosis rate was calculated by cutting the tumors into half along the longitudinal axis and measuring the maximum diameters of the intratumoral necrotic regions. The average tumor volume in the three groups increased to different degrees at each time point; however, the average tumor growth rates in groups B and C were substantially lower than that in group A, exhibiting a statistically significant difference. The difference in the tumor growth rate between group B and group C was not statistically significant. The serum concentrations of IL-6 and TNF-α increased in the three groups at each time point, with larger increases occurring in groups B and C; however, the greater increases did not reach statistical significance. Although the diameters of the necrotic areas were larger in both groups B and C than that in group A, significant differences in necrotic area diameters were only found when comparing groups A and C on days 4 and 12 following treatment. Direct injection of different concentrations of ozonated saline into VX2 tumors significantly increased intratumoral necrosis and reduced the tumor growth rate. The associated mechanism may be partially mediated by IL-6 and TNF-α, as the serum concentrations of these molecules increased after the treatment.
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http://dx.doi.org/10.19779/j.cnki.2096-3602.2018.03.03DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586575PMC
August 2018

Clinical Features and Laboratory Examination to Identify Severe Patients with COVID-19: A Systematic Review and Meta-Analysis.

Biomed Res Int 2021 15;2021:6671291. Epub 2021 Nov 15.

The First Hospital of China Medical University, Shenyang, 110001 Liaoning, China.

Background: With the COVID-19 epidemic breakout in China, up to 25% of diagnosed cases are considered to be severe. To effectively predict the progression of COVID-19 via patients' clinical features at an early stage, the prevalence of these clinical factors and their relationships with severe illness were assessed.

Methods: In this study, electronic databases (PubMed, Embase, Web of Science, and Chinese database) were searched to obtain relevant studies, including information on severe patients. Publication bias analysis, sensitivity analysis, prevalence, sensitivity, specificity, likelihood ratio, diagnosis odds ratio calculation, and visualization graphics were achieved through software Review Manager 5.3, Stata 15, Meta-DiSc 1.4, and R.

Results: Data of 3.547 patients from 24 studies were included in this study. The results revealed that patients with chronic respiratory system diseases (pooled positive likelihood 6.07, 95% CI: 3.12-11.82), chronic renal disease (4.79, 2.04-11.25), cardiovascular disease (3.45, 2.19-5.44), and symptoms of the onset of chest tightness (3.8, 1.44-10.05), shortness of breath (3.18, 2.24-4.51), and diarrhea (2.04, 1.38-3.04) exhibited increased probability of progressing to severe illness. C-reactive protein, ratio of neutrophils to lymphocytes, and erythrocyte sedimentation rate increased a lot in severe patients compared to nonsevere. Yet, it was found that clinical features including fever, cough, and headache, as well as some comorbidities, have little warning value.

Conclusions: The clinical features and laboratory examination could be used to estimate the process of infection in COVID-19 patients. The findings contribute to the more efficient prediction of serious illness for patients with COVID-19 to reduce mortality.
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http://dx.doi.org/10.1155/2021/6671291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593588PMC
November 2021

An App knock-in rat model for Alzheimer's disease exhibiting Aβ and tau pathologies, neuronal death and cognitive impairments.

Cell Res 2021 Nov 17. Epub 2021 Nov 17.

School of Pharmaceutical Sciences, IDG/McGovern Institute for Brain Research, Tsinghua University-Peking University Joint Center for Life Sciences, Tsinghua University, Beijing, China.

A major obstacle in Alzheimer's disease (AD) research is the lack of predictive and translatable animal models that reflect disease progression and drug efficacy. Transgenic mice overexpressing amyloid precursor protein (App) gene manifest non-physiological and ectopic expression of APP and its fragments in the brain, which is not observed in AD patients. The App knock-in mice circumvented some of these problems, but they do not exhibit tau pathology and neuronal death. We have generated a rat model, with three familiar App mutations and humanized Aβ sequence knocked into the rat App gene. Without altering the levels of full-length APP and other APP fragments, this model exhibits pathologies and disease progression resembling those in human patients: deposit of Aβ plaques in relevant brain regions, microglia activation and gliosis, progressive synaptic degeneration and AD-relevant cognitive deficits. Interestingly, we have observed tau pathology, neuronal apoptosis and necroptosis and brain atrophy, phenotypes rarely seen in other APP models. This App knock-in rat model may serve as a useful tool for AD research, identifying new drug targets and biomarkers, and testing therapeutics.
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http://dx.doi.org/10.1038/s41422-021-00582-xDOI Listing
November 2021

Yield-Related QTL Clusters and the Potential Candidate Genes in Two Wheat DH Populations.

Int J Mol Sci 2021 Nov 3;22(21). Epub 2021 Nov 3.

Australian-China Joint Centre for Wheat Improvement, Agricultural Sciences, College of Science, Health, Engineering and Education, Murdoch University, South Street, 90, Murdoch, WA 6150, Australia.

In the present study, four large-scale field trials using two doubled haploid wheat populations were conducted in different environments for two years. Grain protein content (GPC) and 21 other yield-related traits were investigated. A total of 227 QTL were mapped on 18 chromosomes, which formed 35 QTL clusters. The potential candidate genes underlying the QTL clusters were suggested. Furthermore, adding to the significant correlations between yield and its related traits, correlation variations were clearly shown within the QTL clusters. The QTL clusters with consistently positive correlations were suggested to be directly utilized in wheat breeding, including 1B.2, 2A.2, 2B (4.9-16.5 Mb), 2B.3, 3B (68.9-214.5 Mb), 4A.2, 4B.2, 4D, 5A.1, 5A.2, 5B.1, and 5D. The QTL clusters with negative alignments between traits may also have potential value for yield or GPC improvement in specific environments, including 1A.1, 2B.1, 1B.3, 5A.3, 5B.2 (612.1-613.6 Mb), 7A.1, 7A.2, 7B.1, and 7B.2. One GPC QTL (5B.2: 671.3-672.9 Mb) contributed by cultivar Spitfire was positively associated with nitrogen use efficiency or grain protein yield and is highly recommended for breeding use. Another GPC QTL without negatively pleiotropic effects on 2A (50.0-56.3 Mb), 2D, 4D, and 6B is suggested for quality wheat breeding.
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http://dx.doi.org/10.3390/ijms222111934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585063PMC
November 2021

The high diversity of SARS-CoV-2-related coronaviruses in pangolins alerts potential ecological risks.

Zool Res 2021 11;42(6):834-844

State Key Laboratory of Genetic Resources and Evolution, Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650201, China.

Understanding the zoonotic origin and evolution history of SARS-CoV-2 will provide critical insights for alerting and preventing future outbreaks. A significant gap remains for the possible role of pangolins as a reservoir of SARS-CoV-2 related coronaviruses (SC2r-CoVs). Here, we screened SC2r-CoVs in 172 samples from 163 pangolin individuals of four species, and detected positive signals in muscles of four and, for the first time, one . Phylogeographic analysis of pangolin mitochondrial DNA traced their origins from Southeast Asia. Using in-solution hybridization capture sequencing, we assembled a partial pangolin SC2r-CoV (pangolin-CoV) genome sequence of 22 895 bp (MP20) from the sample. Phylogenetic analyses revealed MP20 was very closely related to pangolin-CoVs that were identified in seized by Guangxi Customs. A genetic contribution of bat coronavirus to pangolin-CoVs via recombination was indicated. Our analysis revealed that the genetic diversity of pangolin-CoVs is substantially higher than previously anticipated. Given the potential infectivity of pangolin-CoVs, the high genetic diversity of pangolin-CoVs alerts the ecological risk of zoonotic evolution and transmission of pathogenic SC2r-CoVs.
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http://dx.doi.org/10.24272/j.issn.2095-8137.2021.334DOI Listing
November 2021

Nano-metal-organic-frameworks for treating HO-Secreting bacteria alleviate pulmonary injury and prevent systemic sepsis.

Biomaterials 2021 Nov 2;279:121237. Epub 2021 Nov 2.

Department of Radiology, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, PR China; Intelligent Nanomedicine Institute, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China. Electronic address:

As a vital bacteria-secreted toxin, hydrogen peroxide (HO) can destroy infected tissues and increase vascular permeability, leading to life-threatening systemic bacteremia or sepsis. No strategy that can alleviate HO-induced injury and prevent systemic sepsis has been reported. Herein, as a proof of concept, we demonstrate the use of HO-reactive metal-organic framework nanosystems (MOFs) for treating HO-secreting bacteria. In mice infected with Streptococcus pneumoniae (S. pneumoniae) isolated from patients, MOFs efficiently accumulate in the lungs after systemic administration due to infection-induced alveolar-capillary barrier dysfunction. Moreover, MOFs sequester pneumococcal HO, reduce endothelial DNA damage, and prevent systemic dissemination of bacteria. In addition, this nanosystem exhibits excellent chemodynamic bactericidal effects against drug-resistant bacteria. Through synergistic therapy with the antibiotic ampicillin, MOFs eliminate over 98% of invading S. pneumoniae, resulting in a survival rate of greater than 90% in mice infected with a lethal dose of S. pneumoniae. This work opens up new paths for the clinical treatment of toxin-secreting bacteria.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121237DOI Listing
November 2021

Effect of inulin on pasting, thermal, rheological properties and in vitro digestibility of pea starch gel.

Int J Biol Macromol 2021 Nov 3. Epub 2021 Nov 3.

College of Food and Bioengineering, Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China. Electronic address:

The influence of inulin (IN) on pasting, thermal, rheological properties, and in vitro digestibility of pea starch gel was investigated. Results showed that as the concentration of IN in PS increased, the pasting temperature of PS gradually increased, while the value of peak viscosity, breakdown, and setback decreased. Rheological test suggested that all PS-IN gels were typical non-Newtonian fluids and exhibited a solid-like behavior. With the increased concentration of IN, hardness, chewiness, and gumminess of PS-IN gels significantly declined, in which the minimum value was at addition level of 20%. The presence of IN increased the gelatinization temperatures of PS-IN gels, while decreased the gelatinization enthalpy. Fourier-transform infrared spectroscopy (FT-IR) results indicated that no covalent bonding but intermolecular hydrogen bonding occurred between PS and IN. No influence of IN on the diffraction peak of PS after pasting was confirmed by X-ray diffraction analysis. In addition, IN decreased the content of rapidly and slowly digestible starch of PS, while increasing the content of resistant starch. These results will expand the application range of PS, and also provide a theoretical basis for the development of inulin-starch based products.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.11.004DOI Listing
November 2021

Tumour suppressor TET2 safeguards enhancers from aberrant DNA methylation and epigenetic reprogramming in ERα-positive breast cancer cells.

Epigenetics 2021 Oct 30:1-15. Epub 2021 Oct 30.

Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, and Shanghai Key Laboratory of Medical Epigenetics, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

Aberrant DNA methylation is an epigenetic hallmark of malignant tumours. The DNA methylation level is regulated by not only DNA methyltransferases (DNMTs) but also Ten-Eleven Translocation (TET) family proteins. However, the exact role of genes in breast cancer remains controversial. Here, we uncover that the ERα-positive breast cancer patients with high mRNA expression had better overall survival rates. Consistently, knockout of promotes the tumorigenesis of ERα-positive MCF7 breast cancer cells. Mechanistically, TET2 loss leads to aberrant DNA methylation (gain of 5mC) at a large proportion of enhancers, accompanied by significant reduction in H3K4me1 and H3K27ac enrichment. By analysing the epigenetically reprogrammed enhancers, we identify oestrogen responsive element () as one of the enriched motifs of transcriptional factors. Importantly, TET2 loss impairs 17beta-oestradiol (E2)-induced transcription of the epigenetically reprogrammed -associated genes through attenuating the binding of ERα. Taken together, these findings shed light on our understanding of the epigenetic mechanisms underlying the enhancer reprogramming during breast cancer pathogenesis.
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http://dx.doi.org/10.1080/15592294.2021.1997405DOI Listing
October 2021

ALKBH5 facilitates hypoxia-induced paraspeckle assembly and IL8 secretion to generate an immunosuppressive tumor microenvironment.

Cancer Res 2021 Oct 20. Epub 2021 Oct 20.

Department of Cell Biology, Tianjin Medical University

The dynamic changes of RNA N6-methyl-adenosine (m6A) during cancer progression contribute to quick adaption to microenvironmental changes. Here, we profiled the cancer cell m6A dynamics in the hypoxic tumor niche and its pathological consequences in glioblastoma multiforme (GBM). The m6A demethylase ALKBH5 was induced in GBM models under hypoxic conditions and was associated with a hypoxic gene signature in GBM patient samples. Depletion or inactivation of ALKBH5 in GBM cells significantly suppressed hypoxia-induced tumor-associated macrophage (TAM) recruitment and immunosuppression in allograft tumors. Expression and secretion of CXCL8/IL8 was significantly suppressed in ALKBH5-deficient tumors. However, ALKBH5 did not regulate CXCL8 m6A directly. Instead, hypoxia-induced ALKBH5 erased m6A deposition from the lncRNA NEAT1, stabilizing the transcript and facilitating NEAT1-mediated paraspeckle assembly, which led to relocation of the transcriptional repressor SFPQ from the CXCL8 promoter to paraspeckles and, ultimately, upregulation of CXCL8/IL8 expression. Accordingly, ectopic expression of CXCL8 in ALKBH5-deficient GBM cells partially restored TAM recruitment and tumor progression. Together, this study links hypoxia-induced epitranscriptomic changes to the emergence of an immunosuppressive microenvironment facilitating tumor evasion.
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http://dx.doi.org/10.1158/0008-5472.CAN-21-1456DOI Listing
October 2021

Characteristics, distribution, and children exposure assessment of 13 metals in household dust in China: A big data pilot study.

Indoor Air 2021 Oct 18. Epub 2021 Oct 18.

China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing, China.

To explore the pollution characteristics of metals in household dust in China and their exposure to children, this study searched peer-reviewed papers published during 1980-2020 and analyzed 30 eligible papers screened under the per-decided strategy. We evaluated the sample-weighted concentration (SWC) of each metal, explored the sources of metals, and presented the quantitative description of spatial-temporary characteristics and children exposure to 13 metals with multi-route under a general living scenario. The results showed the concentrations of 13 metals with a range of 0.89-29 090.19 mg/kg. The SWC of Cd in household dust from rural areas was 3.29 times of that from urban areas, while the SWC of Ni from urban areas was 3.71 times of that from rural areas. The results showed that four principal components were extracted, and the cumulative contribution rate reached 79.127%. The exposure dose of 13 metals to children aged 2-3 years was presented with the highest by ingestion. Metals such as Fe, Zn, and Mn posed inevitable health risk to children with high exposure. Countermeasures should be carried out to minimize the children exposure to metals in household dust urgently, such as the establishment of environmental health standard for household dust.
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http://dx.doi.org/10.1111/ina.12943DOI Listing
October 2021

Characterization and fine mapping of a lesion mimic mutant (Lm5) with enhanced stripe rust and powdery mildew resistance in bread wheat (Triticum aestivum L.).

Theor Appl Genet 2021 Oct 18. Epub 2021 Oct 18.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.

Key Message: A novel light intensity-dependent lesion mimic mutant with enhanced disease resistance was physiologically, biochemically, and genetically characterized, and the causative gene was fine mapped to a 1.28 Mbp interval containing 17 high-confidence genes. Lesion mimic mutants are ideal for studying disease resistance and programmed cell death photosynthesis in plants to improve crop yield. In this study, a novel light intensity-dependent lesion mimic mutant (MC21) was obtained from the wheat variety Chuannong16 (CN16) by ethyl methane sulfonate treatment. The mutant initially developed tiny lesion spots on the basal part of the leaves, which then gradually proceeded down to leaf sheaths, stems, shells, and awns at the flowering stage. The major agronomic traits were significantly altered in the mutant compared to that in the wild-type CN16. Furthermore, the mutant exhibited a lesion phenotype with degenerated chloroplast structure, decreased chlorophyll content, increased level of reactive oxygen species, and increased resistance to stripe rust and powdery mildew. Genetic analysis indicated that the lesion phenotype was controlled by a novel single semi-dominant nuclear gene. The target gene was mapped on chromosome arm 2AL located between Kompetitive Allele Specific PCR (KASP) markers, KASP-4211 and KASP-5353, and tentatively termed as lesion mimic 5 (Lm5). The fine mapping suggested that Lm5 was located in a 1.28 Mbp interval between markers KASP-5825 and KASP-9366; 17 high-confidence candidate genes were included in this genomic region. This study provides an important foundational step for further cloning of Lm5 using a map-based approach.
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http://dx.doi.org/10.1007/s00122-021-03973-1DOI Listing
October 2021

Characterization of the Durum Wheat- 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance.

Front Plant Sci 2021 30;12:745290. Epub 2021 Sep 30.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Durum wheat is one of the important food and cash crops. The main goals in current breeding programs are improving its low yield potential, kernel characteristics, and lack of resistance or tolerance to some biotic and abiotic stresses. In this study, a nascent synthesized hexaploid wheat Lanmai/AT23 is used as the female parent in crosses with its AB genome donor Lanmai. A tetraploid line YL-443 with supernumerary spikelets and high resistance to stripe rust was selected out from the pentaploid F progeny. Somatic analysis using multicolor fluorescence hybridization (mc-FISH) revealed that this line is a disomic substitution line with the 4B chromosome pair of Lanmai replaced by the 4D chromosome pair of AT23. Comparing with Lanmai, YL-443 shows an increase in the number of spikelets and florets per spike by 36.3 and 75.9%, respectively. The stripe rust resistance gene carried on the 4D chromosome was fully expressed in the tetraploid background. The present 4D(4B) disomic substitution line YL-443 was distinguished from the previously reported 4D(4B) lines with the 4D chromosomes from Chinese Spring (CS). Our study demonstrated that YL-443 can be used as elite germplasm for durum wheat breeding targeting high yield potential and stripe rust resistance. The -specific PCR marker and the 4D chromosome-specific KASP markers together with its unique features of pubescent leaf sheath and auricles can be utilized for assisting selection in breeding.
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http://dx.doi.org/10.3389/fpls.2021.745290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514839PMC
September 2021

Volume and surface coil simultaneous reception (VSSR) method for intensity inhomogeneity correction in MRI.

Technol Health Care 2021 Sep 29. Epub 2021 Sep 29.

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.

Background: Addressing intensity inhomogeneity is critical in magnetic resonance imaging (MRI) because associated errors can adversely affect post-processing and quantitative analysis of images (i.e., segmentation, registration, etc.), as well as the accuracy of clinical diagnosis. Although several prior methods have been proposed to eliminate or correct intensity inhomogeneity, some significant disadvantages have remained, including alteration of tissue contrast, poor reliability and robustness of algorithms, and prolonged acquisition time.

Objective: In this study, we propose an intensity inhomogeneity correction method based on volume and surface coils simultaneous reception (VSSR).

Methods: The VSSR method comprises of two major steps: 1) simultaneous image acquisition from both volume and surface coils and 2) denoising of volume coil images and polynomial surface fitting of bias field. Extensive in vivo experiments were performed considering various anatomical structures, acquisition sequences, imaging resolutions, and orientations. In terms of correction performance, the proposed VSSR method was comparatively evaluated against several popular methods, including multiplicative intrinsic component optimization and improved nonparametric nonuniform intensity normalization bias correction methods.

Results: Experimental results show that VSSR is more robust and reliable and does not require prolonged acquisition time with the volume coil.

Conclusion: The VSSR may be considered suitable for general implementation.
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http://dx.doi.org/10.3233/THC-213149DOI Listing
September 2021

A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.

Plant Methods 2021 Oct 16;17(1):106. Epub 2021 Oct 16.

Joint International Research Laboratory of Metabolic and Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Background: The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high efficiency. Despite the efforts have made on Artemisia annua transient expression, achieving high-throughput gene functional characterization basing on a fast and easy-manipulated transient transformation system in A. annua remains challenging.

Results: The first pair of true leaves of A. annua is an ideal candidate for Agrobacterium injection. EHA105 was the optimal strain that can be used for the development of the transient expression system. The supplementation of Triton X-100 at a concentration of 0.005% greatly improved the transient expression frequency. According to the histochemical β-Glucuronidase (GUS) staining assay, high transient expression level of the reporter gene (GUS) maintained at least a week. Dual-luciferase (Dual-LUC) transient assays showed that the activity of cauliflower mosaic virus 35S (CaMV35S) promoter and its derivates varied between A. annua and tobacco. In A. annua, the CaMV35S promoter had comparable activity with double CaMV35S promoter, while in tobacco, CaMV35S exhibited approximately 50% activity of double CaMV35S promoter. Otherwise, despite the CaMV35S promoter and double CaMV35S promoter from GoldenBraid Kit 2.0 displayed high activity strength in tobacco, they demonstrated a very low activity in transiently expressed A. annua. The activity of UBQ10 promoter and endogenous UBQb promoter was investigated as well. Additionally, using our transient expression system, the transactivation of AaGSW1 and AaORA on AaCYP71AV1 promoter was confirmed. Dual-LUC assays demonstrated that AaHD8 activated the expression of two glandular secreting trichomes-specific lipid transfer protein genes AaLTP1 and AaLTP2, indicating that AaLTP1 and AaLTP2 might serve as downstream components of AaHD8-involved glandular trichome initiation and cuticle formation, as well as artemisinin secretion in A. annua.

Conclusions: A simple, rapid, good-reproducibility, high-efficiency and low-cost transient transformation system in A. annua was developed. Our method offered a new way for gene functional characterization studies such as gene subcellular localization, promoter activity and transcription activation assays in A. annua, avoiding the aberrant phenotypes resulting from gene expression in a heterologous system.
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http://dx.doi.org/10.1186/s13007-021-00807-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520255PMC
October 2021

The Gut Microbiome and Sex Hormone-Related Diseases.

Front Microbiol 2021 28;12:711137. Epub 2021 Sep 28.

The Affiliated Hospital of Southwest Medical University, Luzhou, China.

The role of the gut microbiome has been a hot topic in recent years. One aim of this review is to shed light on the crosstalk between sex hormones and the gut microbiome. Researchers have observed a sex bias of the composition of the gut microbiome in mice and have proved that sex differences influence the composition of the gut microbiome, although the influence is usually obscured by genetic variations. cell studies, animal studies and some observational studies in humans, researchers have confirmed that the gut microbiome can be shaped by the hormonal environment. On other hand, some theories suggest that the gut microbiota regulates the levels of sex hormones interactions among its metabolites, the immune system, chronic inflammation and some nerve-endocrine axes, such as the gut-brain axis. In addition, bidirectional interactions between the microbiome and the hormonal system have also been observed, and the mechanisms of these interactions are being explored. We further describe the role of the gut microbiome in sex hormone-related diseases, such as ovarian cancer, postmenopausal osteoporosis (PMOP), polycystic ovary syndrome and type 1 diabetes. Among these diseases, PMOP is described in detail. Finally, we discuss the treatments of these diseases and the application prospects of microbial intervention.
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http://dx.doi.org/10.3389/fmicb.2021.711137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506209PMC
September 2021

Prevention and control of coronavirus disease 2019 (COVID-19) in public places.

Environ Pollut 2022 Jan 8;292(Pt B):118273. Epub 2021 Oct 8.

National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, China. Electronic address:

Public places favor the transmission of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) due to dense population, large personal mobility, and higher contact opportunities. In order to protect the health of general public in operating public places during COVID-19 pandemic, this study proposed general precautions and control strategies from perspective of operation management, social distancing, cleaning and disinfection, and personal protection. In addition, with regard of risk level, specific precautions and control strategies were proposed for living service places, outdoor places, and confined places. The comprehensive application of above recommendations could effectively interrupt the spread of COVID-19, and protect the health of general public in public places. This study proposed general and specific precautions and control strategies in public places during COVID-19, and suggested further improvement of pandemic response.
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http://dx.doi.org/10.1016/j.envpol.2021.118273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498926PMC
January 2022

Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation.

Sci Rep 2021 Oct 5;11(1):19699. Epub 2021 Oct 5.

School of Dentistry, University of Missouri - Kansas City, Kansas City, MO, 64108, USA.

To investigate the ability of theaflavins (TF) from black tea to protect dentin collagen against enzymatic degradation via cross-linking effect under clinically relevant conditions. 10-µm-thick dentin films were microtomed from dentin slabs of human molars. Following demineralization, films or slabs were treated with TF at two concentrations (0.4% and 2%) for 30 s. A well-known collagen cross-linker grape seed proanthocyanidins (PA) was used as control. Collagen cross-linking interactions and stabilization against enzymatic degradation were investigated by Fourier transform infrared spectroscopy, weight loss, hydroxyproline release, and scanning/transmission electron microscopy. Data were analyzed by ANOVA, Tukey's and Student's T test (α = 0.05%). The results showed collagen cross-linking and stabilization efficacy was dependent on TF/PA concentrations. At 2.0%, TF and PA offered nearly full protection to collagen; at 0.4%, TF exhibited a significantly better collagen stabilization effect than PA (P < 0.05), while untreated collagen was completely digested. It's concluded that TF cross-links dentin collagen within a clinically relevant time (30 s) and offers excellent collagen protection against enzymatic degradation, with efficacy comparable to or better than PA. The study supports the potential use of TF as a novel, promising collagen cross-linker for degradation resistant, long-lasting dentin bonding in composite restorations.
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http://dx.doi.org/10.1038/s41598-021-99186-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492614PMC
October 2021

AaWRKY17, a positive regulator of artemisinin biosynthesis, is involved in resistance to Pseudomonas syringae in Artemisia annua.

Hortic Res 2021 Oct 1;8(1):217. Epub 2021 Oct 1.

Joint International Research Laboratory of Metabolic and Developmental Sciences, Frontiers Science Center for Transformative Molecules, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Artemisia annua, a traditional Chinese medicinal plant, remains the only plant source for artemisinin production, yet few genes have been identified to be involved in both the response to biotic stresses, such as pathogens, and artemisinin biosynthesis. Here, we isolated and identified the WRKY transcription factor (TF) AaWRKY17, which could significantly increase the artemisinin content and resistance to Pseudomonas syringae in A. annua. Yeast one-hybrid (Y1H), dual-luciferase (dual-LUC), and electrophoretic mobility shift assay (EMSA) results showed that AaWRKY17 directly bound to the W-box motifs in the promoter region of the artemisinin biosynthetic pathway gene amorpha-4,11-diene synthase (ADS) and promoted its expression. Real-time quantitative PCR (RT-qPCR) analysis revealed that the transcript levels of two defense marker genes, Pathogenesis-Related 5 (PR5) and NDR1/HIN1-LIKE 10 (NHL10), were greatly increased in AaWRKY17-overexpressing transgenic A. annua plants. Additionally, overexpression of AaWRKY17 in A. annua resulted in decreased susceptibility to P. syringae. These results indicated that AaWRKY17 acted as a positive regulator in response to P. syringae infection. Together, our findings demonstrated that the novel WRKY transcription factor AaWRKY17 could potentially be used in transgenic breeding to improve the content of artemisinin and pathogen tolerance in A. annua.
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http://dx.doi.org/10.1038/s41438-021-00652-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484609PMC
October 2021

Electrospun PVP-Core/PHBV-Shell Fibers to Eliminate Tailing Off for an Improved Sustained Release of Curcumin.

Mol Pharm 2021 11 28;18(11):4170-4178. Epub 2021 Sep 28.

School of Materials Science & Engineering, University of Shanghai for Science & Technology, Shanghai 200093, China.

Tailing off release in the sustained release of water-insoluble curcumin (Cur) is a significant challenge in the drug delivery system. As a novel solution, core-shell nanodrug containers have aroused many interests due to their potential improvement in drug-sustained release. In this work, a biodegradable polymer, poly(3-hydroxybutyrate--3-hydroxyvalerate) (PHBV), and hydrophilic polyvinylpyrrolidone (PVP) were exploited as drug delivery carriers by coaxial electrospinning, and the core-shell drug-loaded fibers exhibited improved sustained release of Cur. A cylindrical morphology and a clear core-shell structure were observed by scanning and transmission electron microscopies. The X-ray diffraction pattern and infrared spectroscopy revealed that Cur existed in amorphous form due to its good compatibility with PHBV and PVP. The in vitro drug release curves confirmed that the core-shell container manipulated Cur in a faster drug release process than that in the traditional PHBV monolithic container. The combination of the material and structure forms a novel nanodrug container with a better sustained release of water-insoluble Cur. This strategy is beneficial for exploiting more functional biomedical materials to improve the drug release behavior.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00559DOI Listing
November 2021

[Degradation 2,2',4,4'-Tetrabromodiphenyl Ether by Activated Peroxymonosulfate Using Magnetic Biochar Supported -MnO].

Huan Jing Ke Xue 2021 Oct;42(10):4798-4806

Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China.

Polybrominated diphenyl ethers(PBDEs) are potentially harmful to human health and the ecological environment. It is, therefore, of great significance to develop efficient, economic, and environmentally-friendly advanced oxidation systems for their effective degradation. Here, a magnetic biochar supported manganese dioxide composite(-MnO/MWB) synthesized by hydrothermal methods was used as a catalyst to activate peroxymonosulfate(PMS) in the degradation of 2, 2', 4, 4'-tetrabrominated diphenyl ether(BDE-47). The prepared materials were characterized by SEM, XRD, FT-IR, and BET. The results showed that -MnO/MWB had the best catalytic performance, and the highest degradation efficiency reached 94% under optimal conditions(-MnO/MWB mass ratio=1:2, catalyst dosage=0.05 g·L, PMS concentration=5 mmol·L, BDE-47 concentration=1 mg·L). The effect of initial pH on the system was not distinct, while chloride ions(Cl) and humic acid(HA) inhibited the degradation of BDE-47. In comparison, nitrate ions(NO) and bicarbonate ions(HCO) had no notable effect on the degradation. SO· and·OH were the key free radicals in the degradation of BDE-47 in this system, with SO· being dominant. As showed by the XPS characterization of the materials before and after the reaction, a change in the valence states of Mn and Fe was the main reason for the activation of PMS. It was also revealed that -MnO/MWB composites maintained high catalytic performance after being reused up to four times.
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http://dx.doi.org/10.13227/j.hjkx.202102183DOI Listing
October 2021

Distinct effects of polyphenols and solvents on dentin collagen crosslinking interactions and biostability.

Dent Mater 2021 Sep 24. Epub 2021 Sep 24.

School of Dentistry, University of Missouri - Kansas City, Kansas City, MO, 64108, USA. Electronic address:

Objective: To evaluate the effects of different polyphenols and solvents on dentin collagen's crosslinking interactions and biostabilization against MMPs and collagenase degradation.

Methods: Two polyphenols [proanthocyanidin (PA) and quercetin (QC)] with different water solubility were prepared as treatment solutions using ethanol (EtOH) or dimethyl sulfoxide (DMSO) as solvents. 6-um-thick dentin films were microtomed from dentin slabs of third molars. Following demineralization, films or slabs were subject to 60-s treatment (PA or QC) or no treatment (control) with subsequent extended-rinse with original solvent (EtOH or DMSO) or distilled water (DW). Collagen crosslinking interactions were assessed by FTIR. Biostability was assessed through endogenous MMPs activity via confocal laser scanning microscopy, and exogenous collagenase degradation via weight loss, hydroxyproline release and SEM. Finally, direct collagenase inactivation was also evaluated. Data were analyzed by three-way ANOVA and post-hoc tests (α=0.05%).

Results: Distinct effects of two polyphenols and solvents on collagen crosslinking and biostabilization were observed. Higher crosslinking and biostability efficacy occurred with PA than QC (p<0.001) that demonstrated negligible collagen interactions. With DMSO solvent, efficacy results were significantly reduced with both polyphenols (p<0.05). DMSO-rinse further weakened interactions of PA with collagen, diminishing biostability (p<0.05). Low biostability was detected with QC and DW-rinse, suggesting direct enzymatic inhibition due to physical presence in collagen.

Significance: Collagen crosslinking interactions and biostability depend on polyphenol chemical characteristics. Treatment-solution solvents may affect interactions between polyphenols and collagen, specifically, DMSO showed detrimental effects on collagen crosslinking and biostability and should be used with caution.
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http://dx.doi.org/10.1016/j.dental.2021.09.009DOI Listing
September 2021

Improving norbixin dispersibility and stability by liposomal encapsulation using the pH-driven method.

J Sci Food Agric 2021 Sep 27. Epub 2021 Sep 27.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, PR China.

Background: Norbixin, a carotenoid extracted from annatto seeds, is widely utilized as a natural pigment in foods, cosmetics and medicines. Its water solubility is relatively high under neutral or alkaline conditions but low under acidic conditions, which limits its application in some food products.

Results: This problem was overcome by utilizing liposomes to encapsulate the carotenoids so that they could be easily dispersed within acidic solutions. The norbixin was loaded into the liposomes using the pH-driven method. Liposomes were produced by passing aqueous phospholipid dispersions through a microfluidizer under high pressure. Norbixin was then added to the liposome dispersions at pH 7.0 and then driven into the hydrophobic domains of the phospholipid bilayers by acidifying the system. Measurements of the encapsulation efficiency showed that the norbixin was successfully loaded into the liposomes using the pH-driven method. X-ray diffraction analysis showed that the norbixin was in an amorphous state after incorporation into the liposomes. Encapsulation of norbixin within the liposomes was also shown to increase its water dispersibility and chemical stability under acidic pH conditions.

Conclusion: The pH-driven method therefore provides a useful means of increasing the application of this bioactive carotenoid within functional foods and other products. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11546DOI Listing
September 2021

Green electrospinning of chitin propionate to manufacture nanofiber mats.

Carbohydr Polym 2021 Dec 21;273:118593. Epub 2021 Aug 21.

Composite Materials and Engineering Center, Washington State University, Pullman, WA 99164, USA; Apparel, Merchandising, Design and Textiles, Washington State University, Pullman, WA 99164, USA. Electronic address:

Chitin is the second most abundant biopolymer after cellulose in nature, and it is currently under-utilized partially because of its insolubility in common solvents. Herein, chitin was propionylated to improve its dissolution in green solvents, i.e., ethanol and water, and manufactured nanofibers and nonwoven mats via electrospinning with poly(ethylene oxide) (PEO) as a co-spinning aid. Polymer solution viscosity, electrospun CP/PEO fiber morphology, mechanical, thermal, dynamic thermal, and surface contact angle of nanofiber mats were evaluated. Results showed that fibers with CP content up to 97% could be produced. The electrospun CP/PEO nanofiber mats exhibited good mechanical strength, thermal stability, and hydrophobicity with water contact angles up to 133°. Filtration test of separating carbon nanofibers and carbon nanotubes from water demonstrated the potential use of the CP/PEO nanofiber mats in fluid filtration of fibrous pollutants.
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http://dx.doi.org/10.1016/j.carbpol.2021.118593DOI Listing
December 2021

Patterns and predictors of adolescent life change during the COVID-19 pandemic: a person-centered approach.

Curr Psychol 2021 Sep 13:1-15. Epub 2021 Sep 13.

Institute of Developmental Psychology, Beijing Normal University, No. 19 Xin Jie Kou Wai Street, Hai Dian District, Beijing, 100875 China.

The present study investigated patterns of adolescent life changes across multiple life domains and utilized a holistic-interactionistic perspective to examine their individual, familial, and societal correlates with a sample of 2544 Chinese parent-adolescent dyads. Adolescents were aged from 10 to 19 years old (50.16% girls). Latent profile analysis revealed five life change profiles, including three improved profiles at various degrees, one unchanged profile, and one worsened profile. The majority of adolescents had an improved or unchanged life. Multinomial logistic regression analyses found that most of the individual, familial, and societal factors predicted the group memberships. Notably, parent-adolescent conflict was a significant factor that predicted memberships of all patterns. These findings show the resilience of adolescents and indicate the need for policies and interventions that consider the holistic nature of adolescents' person-context system, especially during a global crisis such as the COVID-19 pandemic.
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http://dx.doi.org/10.1007/s12144-021-02204-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435363PMC
September 2021

Environmental contamination and human exposure of polychlorinated biphenyls (PCBs) in China: A review.

Sci Total Environ 2022 Jan 11;805:150270. Epub 2021 Sep 11.

Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.

Polychlorinated biphenyls (PCBs), together with 11 other organic compounds, were initially listed as persistent organic pollutants (POPs) by the Stockholm Convention because of their potential threat to ecosystems and humans. In China, many monitoring studies have been undertaken to reveal the level of PCBs in environment since 2005 due to the introduced stricter environmental regulations. However, there are still significant gaps in understanding the overall spatial and temporal distributions of PCBs in China. This review systematically discusses the occurrence and distribution of PCBs in environmental matrices, organisms, and humans in China. Results showed that PCB contamination in northern and southern China was not significantly different, but the PCB levels in East China were commonly higher than those in West China, which might have been due to the widespread consumption of PCBs and intensive human activities in East China. Serious PCB contamination was found in e-waste disassembling areas (e.g., Taizhou of Zhejiang Province and Qingyuan and Guiyu of Guangdong Province). Higher PCB concentrations were also chronicled in megalopolises and industrial clusters. The unintentionally produced PCBs (UP-PCBs) formed during industrial thermal processes may play an increasingly significant role in PCB pollution in China. Low PCB levels were recorded in rural and underdeveloped districts, particularly in remote and high-altitude localities such as the Tibetan Plateau and the South China Sea. However, these data are limited. Human exposure to PCBs is closely related to the characteristics of environmental pollution. This review also discusses existing issues and future research prospects on PCBs in China. For instance, the accumulation characteristics and migration regularities of PCBs in food webs should be further studied. More investigations should be undertaken to assess the quantitative relationship between external and internal exposure to PCBs. For example, bioaccessibility and bioavailability studies should be supplemented to evaluate human health risks more accurately.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150270DOI Listing
January 2022

Towards carbon neutrality and water sustainability: An integrated anaerobic fixed-film MBR-reverse osmosis-chlorination process for municipal wastewater reclamation.

Chemosphere 2022 Jan 28;287(Pt 2):132060. Epub 2021 Aug 28.

Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore, 637141, Singapore; School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore. Electronic address:

Freshwater resilience is facing to an increasing challenge, while carbon neutral wastewater reclamation has been put onto agenda in more and more countries. The activated sludge-microfiltration (MF)-reverse osmosis (RO) process has been currently adopted for reclamation of municipal wastewater to high-grade product water (e.g. NEWater). However, the conventional activated sludge (CAS) unit in this process has the drawbacks of excessive sludge generation, high energy consumption, greenhouse gases (GHGs) emissions etc. To address these emerging issues, an integrated anaerobic fixed-film membrane bioreactor (AnfMBR)-RO-chlorination process was developed in this study. Results showed that about 99.9% of COD, 99.3% of phosphate and 95.3% of NH-N were removed in the AnfMBR-RO process, while breakpoint chlorination served as a polishing step when the NH-N concentration in RO permeate exceeded the typical NH-N concentration (e.g. 1 mg/L) of NEWater. The net energy consumption and total GHG emissions in the proposed integrated process were estimated to be 0.33 kWh/m and 310.2 g COe/m influent wastewater treated, respectively, which were 64% and 74% less than those in the current municipal wastewater reclamation process. Consequently, this study offers an alternative path to bring municipal wastewater reclamation one step closer to carbon neutrality and water sustainability.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132060DOI Listing
January 2022

Inhibition of miR-144-3p exacerbates non-small cell lung cancer progression by targeting CEP55.

Acta Biochim Biophys Sin (Shanghai) 2021 Oct;53(10):1398-1407

Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, Hunan University of Medicine, Huaihua 418000, China.

Increasing evidence has indicated that microRNA dysregulation is closely related to the occurrence and development of cancers. Herein, we investigated the relationship between miR-144-3p and CEP55 expression. We then evaluated the association between miR-144-3p and CEP55 expression and proliferation, invasion and apoptosis of non-small cell lung cancer (NSCLC) cells. Real-time quantitative PCR results revealed that CEP55 was over-expressed whereas miR-144-3p was under-expressed in NSCLC tissues. CCK-8 assay, wound healing assay, and flow cytometry further revealed that overexpression of miR-144-3p significantly inhibited proliferation and migration, but promoted apoptosis of A549 cells. Conversely, inhibition of miR-144-3p promoted proliferation and migration but suppressed apoptosis of H460 cells. Dual-luciferase reporter assay revealed that miR-144-3p modulated malignant properties of cancer cells by targeting CEP55. Overexpression of CEP55 partially blocked the inhibitory effect of miR-144-3p on proliferation and migration of A549 cells and induced apoptosis of A549 cells. CEP55 knockdown modulated the increase in proliferation and migration and the decrease in apoptosis of H460 cells following miR-144-3p inhibition. These findings demonstrated that miR-144-3p suppresses NSCLC development by inhibiting CEP55 expression.
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http://dx.doi.org/10.1093/abbs/gmab118DOI Listing
October 2021
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