Publications by authors named "Han Zhou"

393 Publications

Dync1li1 is required for the survival of mammalian cochlear hair cells by regulating the transportation of autophagosomes.

PLoS Genet 2022 Jun 21;18(6):e1010232. Epub 2022 Jun 21.

State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.

Dync1li1, a subunit of cytoplasmic dynein 1, is reported to play important roles in intracellular retrograde transport in many tissues. However, the roles of Dync1li1 in the mammalian cochlea remain uninvestigated. Here we first studied the expression pattern of Dync1li1 in the mouse cochlea and found that Dync1li1 is highly expressed in hair cells (HCs) in both neonatal and adult mice cochlea. Next, we used Dync1li1 knockout (KO) mice to investigate its effects on hearing and found that deletion of Dync1li1 leads to early onset of progressive HC loss via apoptosis and to subsequent hearing loss. Further studies revealed that loss of Dync1li1 destabilizes dynein and alters the normal function of dynein. In addition, Dync1li1 KO results in a thinner Golgi apparatus and the accumulation of LC3+ autophagic vacuoles, which triggers HC apoptosis. We also knocked down Dync1li1 in the OC1 cells and found that the number of autophagosomes were significantly increased while the number of autolysosomes were decreased, which suggested that Dync1li1 knockdown leads to impaired transportation of autophagosomes to lysosomes and therefore the accumulation of autophagosomes results in HC apoptosis. Our findings demonstrate that Dync1li1 plays important roles in HC survival through the regulation of autophagosome transportation.
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http://dx.doi.org/10.1371/journal.pgen.1010232DOI Listing
June 2022

Ghosts as a Vehicle for the Delivery of DNA Vaccines Mediate Immune Responses.

Front Immunol 2022 31;13:849409. Epub 2022 May 31.

Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

We developed bacterial ghosts (BGs) as vehicles for delivering DNA vaccines and analyzed their effects on immune responses. Uptake of the plasmids encoding the enhanced green fluorescent protein (pCI-EGFP) and BGs loaded with pCI-EGFP by macrophages was investigated using fluorescence microscopy and flow cytometry. The results showed that pCI-EGFP-loaded BGs were efficiently taken up by macrophages. BGs loaded with plasmids encoding VP6 protein of PoRV (pCI-PoRV-VP6) significantly upregulated the mRNA expression of interleukin (IL)-1β, IL-10, tumor necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), arginase-1 (Arg-1), Mannose receptor (CD206) toll-like receptor (TLR)-2, TLR4, and TLR9 in macrophages. The levels of markers of M1 polarization (IL-10 and TNF-α) and M2 polarization (Arg-1 and CD206) were increased in macrophages incubated with pCI-PoRV-VP6-loaded BGs compared with the control group. The results of the enzyme-linked immunosorbent assay showed that the secretion of IL-1β, IL-10, and TNF-α in macrophages was significantly upregulated compared with the control group. Flow cytometry demonstrated that BGs loaded with pCI-PoRV-VP6 promoted the maturation of dendritic cells (DCs). Following incubation with pCI-PoRV-VP6-loaded BGs, the mRNA expression levels of IL-1β, IL-6 and interferon (IFN)-γ in DCs were significantly increased. ELISA assay showed the secretion of the IL-1β, IL-6, IFN-γ IL-10 and TNF-α in DCs were upregulated significantly. Thus, BGs promoted the maturation and activation of DCs. We analyzed the stimulatory capacity of DCs in a mixed lymphocyte reaction with allogeneic T cells. T cell proliferation increased upon incubation with DCs stimulated by BGs. After immunizing mice with BGs loaded with pCI-PoRV-VP6, the specific IgG levels in the serum were higher than those elicited by BGs loaded with pCI-PoRV-VP6. BGs loaded with pCI-PoRV-VP6 on Th1 and Th2 cytokines polarized T cells into the Th1 type and increased the proportion of CD4/CD8 T cells. These results indicate BGs effectively mediate immune responses and can be used as delivery system for DNA vaccination.
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http://dx.doi.org/10.3389/fimmu.2022.849409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193971PMC
June 2022

The combination of hydroxychloroquine and 2-deoxyglucose enhances apoptosis in breast cancer cells by blocking protective autophagy and sustaining endoplasmic reticulum stress.

Cell Death Discov 2022 Jun 11;8(1):286. Epub 2022 Jun 11.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

2-Deoxyglucose (2-DG) can be used in antitumour research by inhibiting glycolysis and promoting the endoplasmic reticulum stress (ERS) pathway, but its clinical application is restricted due to dose-limiting side effects and survival chance for cancer cells by protective autophagy. Therefore, our research explored whether the combination of hydroxychloroquine (HCQ), an FDA-approved autophagy inhibiting drug, and 2-DG is a promising therapeutic strategy. Here, we report that HCQ combined with 2-DG can further inhibit the viability and migration and induce apoptosis of breast tumour cells compared with other individual drugs. The combination of 2-DG and HCQ can significantly reduce transplanted tumour size and tumour cell metastasis of the lung and liver in vivo. At the cellular level, HCQ suppressed autolysosome formation and terminated the autophagy process induced by 2-DG-mediated ERS, resulting in the continuous accumulation of misfolded proteins in the endoplasmic reticulum, which generated sustained ERS through the PERK-eIF2α-ATF-4-CHOP axis and triggered the transformation from a survival process to cell death. Our research reinforced the research interest of metabolic disruptors in triple-negative breast cancer and emphasized the potential of the combination of 2-DG and HCQ as an anticancerous treatment.
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http://dx.doi.org/10.1038/s41420-022-01074-6DOI Listing
June 2022

Circular RNA circ_0021001 regulates miR-148b-3p/GREM1 axis to modulate proliferation and apoptosis of vascular smooth muscle cells.

Metab Brain Dis 2022 Jun 11. Epub 2022 Jun 11.

Department of Neurosurgery, Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, 158 Wuyang Avenue, Enshi, Hubei, 445000, China.

Intracranial aneurysm (IA) is an abnormal expression in the intracranial arteries, which is related to the growth and apoptosis of vascular smooth muscle cells (VSMCs). Circular RNA (circRNA) circ_0021001 (also named circARFIP2) has been identified to mediate the regulation of VSMCs proliferation. However, the molecular mechanism of circ_0021001 involved in VSMC dysfunction in IA is poorly defined. The expression levels of circ_0021001, microRNA-148b-3p (miR-148b-3p), and Gremlin 1 (GREM1) were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Cell viability, proliferation, cell cycle progression, and apoptosis were detected by Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), and flow cytometry assays. Protein levels of proliferating cell nuclear antigen (PCNA), p21, B-cell lymphoma-2 (Bcl-2), Bcl-2 related X protein (Bax), and GREM1 were examined by western blot assay. The binding relationship between miR-148b-3p and circ_0021001 or GREM1 was predicted by StarBase and then verified using a dual-luciferase reporter assay. The expression levels of circ_0021001 and GREM1 were increased, and that of miR-148b-3p was decreased in IA tissues and HUASMCs. Moreover, the downregulation of circ_0021001 could repress proliferation ability and induce apoptosis of HUASMCs. The mechanical analysis uncovered that circ_0021001 served as a sponge of miR-148b-3p to regulate GREM1 expression. Circ_0021001 silencing could suppress cell growth and induce apoptosis of HUASMCs partially through modulating the miR-148b-3p/GREM1, presented circ_0021001 as a promising therapeutic target for IA.
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http://dx.doi.org/10.1007/s11011-022-01014-4DOI Listing
June 2022

The Development of Mechanical Allodynia in Diabetic Rats Revealed by Single-Cell RNA-Seq.

Front Mol Neurosci 2022 20;15:856299. Epub 2022 May 20.

Department of Neurosurgery, Ninth People Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Mechanical allodynia (MA) is the main reason that patients with diabetic peripheral neuropathy (DPN) seek medical advice. It severely debilitates the quality of life. Investigating hyperglycemia-induced changes in neural transcription could provide fundamental insights into the complex pathogenesis of painful DPN (PDPN). Gene expression profiles of physiological dorsal root ganglia (DRG) have been studied. However, the transcriptomic changes in DRG neurons in PDPN remain largely unexplored. In this study, by single-cell RNA sequencing on dissociated rat DRG, we identified five physiological neuron types and a novel neuron type MAAC ( ) in PDPN. The novel neuron type originated from peptidergic neuron cluster and was characterized by highly expressing genes related to neurofilament and cytoskeleton. Based on the inferred gene regulatory networks, we found that activated transcription factors and in MAAC could enhance expression. Moreover, we constructed the cellular communication network of MAAC and revealed its receptor-ligand pairs for transmitting signals with other cells. Our molecular investigation at single-cell resolution advances the understanding of the dynamic peripheral neuron changes and underlying molecular mechanisms during the development of PDPN.
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http://dx.doi.org/10.3389/fnmol.2022.856299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165721PMC
May 2022

Predicting retinal sensitivity using optical coherence tomography parameters in central serous chorioretinopathy.

Graefes Arch Clin Exp Ophthalmol 2022 Jun 4. Epub 2022 Jun 4.

Department of Ophthalmology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.

Purpose: To predict changes in retinal sensitivity using optical coherence tomography (OCT) in eyes with central serous chorioretinopathy (CSC).

Methods: Twenty-three eyes in 23 patients with CSC were enrolled. Retinal sensitivity was measured twice using microperimetry in all the examined eyes. Spectral domain OCT measurements were simultaneously conducted. The relationship between retinal sensitivity and the thicknesses of (i) the retinal nerve fiber layer plus the ganglion cell layer (RNFL + GCL), (ii) the inner nuclear layer (INL), (iii) the outer nuclear layer (ONL), and (iv) the serous retinal detachment height (SRDH) were investigated in a point-wise manner. The associations between the change in retinal sensitivity and the OCT parameters at baseline were also investigated.

Results: The mean age of the participants was 49.8 ± 10.7 years. The mean SRDH was significantly lower (p < 0.001), and the mean retinal sensitivity (p < 0.001) was significantly higher at the second examination, compared with the first; however, the logMAR visual acuity (VA) did not differ significantly between the two examinations (p = 0.063). The logMAR VA was associated with retinal sensitivity at both the first and second examinations (p < 0.001). The retinal sensitivity at the second examination was significantly correlated with the retinal sensitivity, RNFL + GCL, INL, ONL, and SRDH at the first examination and with the improvement in SRDH.

Conclusions: Retinal sensitivity was associated with the retinal structure in eyes with CSC; these parameters could be useful for predicting the change in visual function prior to treatment.
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http://dx.doi.org/10.1007/s00417-022-05717-3DOI Listing
June 2022

Re-Du-Ning injection ameliorates radiation-induced pneumonitis and fibrosis by inhibiting AIM2 inflammasome and epithelial-mesenchymal transition.

Phytomedicine 2022 Jul 21;102:154184. Epub 2022 May 21.

State Key Laboratory of Pharmaceutical Biotechnology, Deparment of Biotechnology and Pharmaceutical Sciences, School of Life Sciences, Nanjing University, 163 Xianlin Avenue, Nanjing 210023, China. Electronic address:

Background: Radiation-induced lung injury (RILI) is a common side effect in chest radiotherapy patients, and there is no good medicine to treat it. Re-Du-Ning (RDN) injection is a traditional Chinese medicine that is clinically used to treat upper respiratory tract infections and acute bronchitis. RDN has the advantage of high safety and mild side effects. The mechanism of most traditional Chinese medicine preparations is unknown.

Purpose: To illustrate the mechanisms of RDN for the treatment of RILI.

Methods: Female C57BL/6 mice were used to establish a RILI model via irradiation, and RDN injection was intraperitoneally administered at doses of 5, 10, and 20 ml/kg. The cytokines were measured by ELISA and qPCR. The data related to Absent in melanoma 2 (AIM2) inflammasome were analyzed via ELISA and a network pharmacological approach. In addition, the data related to epithelial-mesenchymal transition (EMT) were analyzed via immunofluorescence, Western blotting, and a network pharmacological approach.

Results: RDN robustly alleviated RILI. Meanwhile, RDN downregulated inflammatory cells' infiltration and the expression of pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α. Next, the potential molecular mechanisms of RDN were predicted through network pharmacology analysis. RDN may ameliorate radiation pneumonitis (RP) by inhibiting AIM2-mediated pyroptosis. Moreover, RDN treatment inhibited EMT and phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT) pathway. The active compounds from Lonicera japonica Thunb. decreased the phosphorylation of Akt.

Conclusion: These findings demonstrate that RDN, as a traditional Chinese medicine preparation, will be a candidate drug for treating RILI.
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http://dx.doi.org/10.1016/j.phymed.2022.154184DOI Listing
July 2022

Denoising the hob vibration signal using improved complete ensemble empirical mode decomposition with adaptive noise and noise quantization strategies.

ISA Trans 2022 May 27. Epub 2022 May 27.

State Key Laboratory of Mechanical Transmission, Chongqing University, Chongqing 400044, China.

A novel denoising method combining complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN) and noise quantization strategies is proposed to solve the problem of the noise of the hob vibration signals disturbing the condition monitoring and feature extraction. The vibration signal is decomposed into several intrinsic mode functions (IMFs) and a residual based on CEEMDAN first. Considering that statistical indicators such as correlation coefficient and kurtosis are not effective in the presence of non-Gaussian noises and modulation because they primarily focus on the signal statistical distribution while ignoring the characteristics of the mechanism, a novel index based on the autocorrelation function analysis called periodic modulation for noise assessment (PMNA) is proposed to quantify the noise of IMFs. Further, IMFs are rearranged in the decreasing order of PMNA. A novel threshold joint with IMFs noise assessment (TJINA) varying with the combination of PMNA and the rearranged IMF retrieval is designed, which has advantages in the local smoothness and small fluctuation. On that basis, IMFs are divided into noise domain and signal domain, IMFs in the noise domain are denoised with TJINA and soft threshold function strategies. The proposed method is applied to the simulated signals with different input signal to noise ratios (SNR) and two measured gear hobbing vibration signals. The comparison with some state-of-the-art approaches and the ablation experiment reveals that the proposed method performs better in enhancing the effective components and eliminating noise.
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http://dx.doi.org/10.1016/j.isatra.2022.05.017DOI Listing
May 2022

Overcoming Reproductive Compromise Under Heat Stress in Wheat: Physiological and Genetic Regulation, and Breeding Strategy.

Front Plant Sci 2022 13;13:881813. Epub 2022 May 13.

National Engineering Laboratory of Crop Stress Resistance Breeding, School of Agronomy, Anhui Agricultural University, Hefei, China.

The reproductive compromise under heat stress is a major obstacle to achieve high grain yield and quality in wheat worldwide. Securing reproductive success is the key solution to sustain wheat productivity by understanding the physiological mechanism and molecular basis in conferring heat tolerance and utilizing the candidate gene resources for breeding. In this study, we examined the performance on both carbon supply source (as leaf photosynthetic rate) and carbon sink intake (as grain yields and quality) in wheat under heat stress varying with timing, duration, and intensity, and we further surveyed physiological processes from source to sink and the associated genetic basis in regulating reproductive thermotolerance; in addition, we summarized the quantitative trait loci (QTLs) and genes identified for heat stress tolerance associated with reproductive stages. Discovery of novel genes for thermotolerance is made more efficient the combination of transcriptomics, proteomics, metabolomics, and phenomics. Gene editing of specific genes for novel varieties governing heat tolerance is also discussed.
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http://dx.doi.org/10.3389/fpls.2022.881813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137415PMC
May 2022

Downregulation of NF-κB by Shp-1 alleviates cerebral venous sinus thrombosis-induced brain edema via suppression of AQP4.

J Stroke Cerebrovasc Dis 2022 May 25;31(8):106570. Epub 2022 May 25.

Department of Neurosurgery, The Affiliated Hospital of Qingdao University, 16 Jiangsu Street, Qingdao 266000, China. Electronic address:

Aquaporin 4 (AQP4), a water channel protein, has been well studied in arterial stroke-induced brain edema. However, the role of AQP4 in cerebral venous sinus thrombosis (CVST) has not been reported. Here, we showed that AQP4 expression was increased in the brain of a rat CVST model, whereas inhibition of AQP4 decreased cerebral edema. Subsequent experiments showed that Shp-1 (Src homology 2-containing phosphatase-1) expression and NF-κB phosphorylation were upregulated after CVST. We found that Shp-1 inhibition resulted in enhancement of NF-κB activation and increased AQP4 expression accompanied by aggravated brain edema. We further showed that NF-κB inhibition led to decreased AQP4 expression and subsequent attenuation of brain edema but had no significant effect on Shp-1 expression. These results provide the first evidence suggesting that downregulation of NF-κB by Shp-1 alleviates CVST-induced brain edema through suppression of AQP4.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2022.106570DOI Listing
May 2022

Evaluation of the Immunogenicity in Mice Orally Immunized with Recombinant Expressing Porcine Epidemic Diarrhea Virus S1 Protein.

Viruses 2022 04 25;14(5). Epub 2022 Apr 25.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.

Porcine epidemic diarrhea (PED), characterized by diarrhea, vomiting, and dehydration, is an acute enteric infectious disease of pigs. The disease is caused by porcine epidemic diarrhea virus (PEDV), which infects the intestinal mucosal surface. Therefore, mucosal immunization through the oral route is an effective method of immunization. Lactic acid bacteria, which are acid resistant and bile-salt resistant and improve mucosal immunity, are ideal carriers for oral vaccines. The S1 glycoprotein of PEDV mediates binding of the virus with cell receptors and induces neutralizing antibodies against the virus. Therefore, we reversely screened the recombinant strain pPG-SD-S1/Δ expressing PEDV S1 glycoprotein by deficient in genotype (Δ). Mice were orally immunized three times with the recombinant bacteria that had been identified for expression, and the changes of anti-PEDV IgG and secreted immunoglobulin A levels were observed over 70 days. The results indicated that the antibody levels notably increased after oral administration of recombinant bacteria. The detection of extracellular cytokines on the 42nd day after immunization indicated high levels of humoral and cellular immune responses in mice. The above results demonstrate that pPG-SD-S1/Δ has great potential as an oral vaccine against PEDV.
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http://dx.doi.org/10.3390/v14050890DOI Listing
April 2022

Oral Immunization of Chickens with Probiotic Constitutively Expressing the α-β2-ε-β1 Toxoids to Induce Protective Immunity.

Vaccines (Basel) 2022 Apr 29;10(5). Epub 2022 Apr 29.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.

() is a bacterium that commonly causes zoonotic disease. The pathogenicity of is a result of the combined action of α, β, and ε exotoxins. In this study, (pPG-T7g10/) expressing the main toxoids of , α, ε, β1, and β2, with EGFP-labeling, was constructed, and the protective effect was estimated in chickens. The α-β2-ε-β1 toxoid was constitutively expressed for confirmation by laser confocal microscopy and western blotting, and its immunogenicity was analyzed by enzyme-linked immunosorbent assay (ELISA) and immunohistochemical assays. After booster immunization, the probiotic vaccine group showed significantly higher levels ( < 0.05) of specific secretory IgA (sIgA) and IgY antibodies in the serum and intestinal mucus. Furthermore, the levels of cytokines, including interferon (IFN)-γ, interleukin (lL)-2, IL-4, IL-10, IL-12, and IL-17, and the proliferation of spleen lymphocytes in chickens orally immunized with pPG-E-α-β2-ε-β1/ increased significantly. Histopathological observations showed that the intestinal pathological changes in chickens immunized with pPG-E-α-β2ε-β1/ were significantly alleviated. These data reveal that the probiotic vaccine could stimulate mucosal, cellular, and humoral immunity and provide an active defense against the toxins of , suggesting a promising candidate for oral vaccines against
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http://dx.doi.org/10.3390/vaccines10050698DOI Listing
April 2022

A miniature chemiluminescence spectrometric system induced by atmosphere microplasma jet to avoid using hydrogen peroxide and catalyst.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Oct 18;279:121399. Epub 2022 May 18.

School of Control Engineering, Hebei Key Laboratory of Micro-Nano Precision Optical Sensing and Measurement Technology, Northeastern University at Qinhuangdao, Qinhuangdao 066004, China; College of Information Science and Engineering, Northeastern University, Shenyang 110819, China. Electronic address:

A miniature luminol chemiluminescence system based on atmosphere microplasma is proposed for detection without any catalysts. In our research, atmosphere microplasma jet is employed to oxidize luminol and produce chemiluminescence instead of HO. The transport of OH radicals to the plasma-liquid interface and induce the chemiluminescence. The weight of the system is only 3.6 kg (including a 1.2 kg laptop), and the power consumption of the microplasma is only 0.045 W. The mechanism of luminol chemiluminiscence induced by microplasma jet and generation of microplasma jet are investigated in this study. A 1 mL sample solution is sufficient for trace 3-NPA determination within an analysis time of 6 min. In the range of 0.03-10 mg L, 3-NPA can be quantitatively analyzed along with a detection limit of 0.008 mg L. In addition, the proposed system is employed for real-world samples detection, including water samples, brown sugar and tainted sugarcane, which demonstrates the reliability and practical feasibility of the detection method.
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http://dx.doi.org/10.1016/j.saa.2022.121399DOI Listing
October 2022

Rapid initiation of a single-stage partial nitritation-anammox process treating low-strength ammonia wastewater: Novel insights into biofilm development on porous polyurethane hydrogel carrier.

Bioresour Technol 2022 Aug 21;357:127344. Epub 2022 May 21.

State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai 200092, China.

Media-supported biofilm is a powerful strategy for growth and enrichment of slow-growing microorganisms. In this study, a single-stage nitritation-anammox process treating low-strength wastewater was successfully started to investigate the biofilm development on porous polyurethane hydrogel carrier. Suspended biomass migration into the carrier and being entrapment by its internal interconnected micropores dominated the fast initial colonization stage. Both surface-attached growth and embedded growth of microbes occurred during the following accumulation stage. Fluorescence in situ hybridization analysis of mature biofilm indicated that ammonium-oxidizing bacteria located at the outer layers featured a surface-attached growth, while anammox microcolonies housed in the inner layers proliferated as an embedded-like growth. In this way, the growth rate of anammox bacteria (predominated by Candidatus Kuenenia) could be 0.079 d. The anammox potential of the biofilm reactor reached 1.65 ± 0.3 kg/m/d within two months. This study provides novel insights into nitritation-anammox biofilm formation on the porous polyurethane hydrogel carrier.
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http://dx.doi.org/10.1016/j.biortech.2022.127344DOI Listing
August 2022

Urinary cadmium and peripheral blood telomere length predict the risk of renal function impairment: a study of 547 community residents of Shanxi, China.

Environ Sci Pollut Res Int 2022 May 21. Epub 2022 May 21.

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, 030001, China.

Few reports have investigated the predictive value of urinary cadmium (UCd) and telomere length on renal function impairment. Therefore, we constructed nomogram models, using a cross-sectional survey to analyze the potential function of UCd and telomere length in renal function impairment risk. We randomly selected two community populations in Shanxi, China, and general information of the subjects was collected through face-to-face questionnaire surveys. Venous blood of subjects was collected to detect absolute telomere length (ATL) by real-time quantitative chain reaction (RT-PCR). Collecting urinary samples detected UCd and urinary N-acetyl-β-d-glucosaminidase (UNAG). Estimated glomerular filtration rate (eGFR) was obtained based on serum creatinine (SCr). Nomogram models on risk prediction analysis of renal function impairment was constructed. After adjusting for other confounding factors, UCd (β = 0.853, 95% confidence interval (CI): 0.739 ~ 0.986) and ATL (β = 1.803, 95%CI: 1.017 ~ 1.154) were independent risk influencing factors for increased UNAG levels, and the risk factors for eGFR reduction were UCd (β = 1.011, 95%CI: 1.187 ~ 1.471), age (β = 1.630, 95%CI: 1.303 ~ 2.038), and sex (β = 0.181, 95%CI: 0.105 ~ 0.310). Using UCd, ATL, sex, and age to construct the nomogram, and the C-statistics 0.584 (95%CI: 0.536 ~ 0.632) and 0.816 (95%CI: 0.781 ~ 0.851) were obtained by internal verification of the calibration curve, C-statistics revealed nomogram model validation was good and using decision curve analysis (DCA) confirmed a good predictive value of the nomogram models. In a nomogram model, ATL, UCd, sex, and age were detected as independent risk factors for renal function impairment, with UCd being the strongest predictor.
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http://dx.doi.org/10.1007/s11356-022-20923-6DOI Listing
May 2022

An ultraweak mechanical stimuli actuated single electrode triboelectric nanogenerator with high energy conversion efficiency.

Nanoscale 2022 Jun 1;14(21):7906-7912. Epub 2022 Jun 1.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 101400, China.

Triboelectric nanogenerator (TENG) as a new energy harvester has attracted significant attention due to its excellent output performance and high energy conversion efficiency at low-frequency, small-amplitude and weak-force compared with a traditional electromagnetic generator. Here, an ultraweak mechanical stimuli actuated single electrode triboelectric nanogenerator (UMA-TENG) has been studied with an atomic force microscope. The electrical output and force curve of UMA-TENG were studied at first, as well as the maximum output performance and highest energy conversion efficiency. Then the influence of the driving frequency, separation distance and motion amplitude was investigated, respectively. Moreover, by introducing an external switch to reach a cycle of maximized energy output, the maximum energy conversion efficiency of the UMA-TENG was up to 73.6% with an input mechanical energy of 48 pJ. This work demonstrates that the TENG shows excellent performance in ultraweak mechanical stimuli and could broaden the applications of the TENG in sensors, actuators, micro-robotics, micro-electro-mechanical-systems, and wearable electronics.
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http://dx.doi.org/10.1039/d2nr01530gDOI Listing
June 2022

Genomic insights on the contribution of introgressions from Xian/Indica to the genetic improvement of Geng/Japonica rice cultivars.

Plant Commun 2022 05 13;3(3):100325. Epub 2022 Apr 13.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:

Hybridization between Xian/indica (XI) and Geng/japonica (GJ) rice combined with utilization of plant ideotypes has greatly contributed to yield improvements in modern GJ rice in China over the past 50 years. To explore the genomic basis of improved yield and disease resistance in GJ rice, we conducted a large-scale genomic landscape analysis of 816 elite GJ cultivars representing multiple eras of germplasm from China. We detected consistently increasing introgressions from three XI subpopulations into GJ cultivars since the 1980s and found that the XI genome introgressions significantly increased the grain number per panicle (GN) and decreased the panicle number per plant. This contributed to the improvement of plant type during modern breeding, changing multi-tiller plants to moderate tiller plants with a large panicle size and increasing the blast resistance. Notably, we found that key gene haplotypes controlling plant architecture, yield components, and pest and disease resistance, including IPA1, SMG1, DEP3, Pib, Pi-d2, and Bph3, were introduced from XI rice by introgression. By GWAS analysis, we detected a GN-related gene Gnd5, which had been consistently introgressed from XI into GJ cultivars since the 1980s. Gnd5 is a GRAS transcription factor gene, and Gnd5 knockout mutants showed a significant reduction in GN. The estimated genetic effects of genes varied among different breeding locations, which explained the distinct introgression levels of XI gene haplotypes, including Gnd5, DEP3, etc., to these GJ breeding pedigrees. These findings reveal the genomic contributions of introgressions from XI to the trait improvements of GJ rice cultivars and provide new insights for future rice genomic breeding.
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http://dx.doi.org/10.1016/j.xplc.2022.100325DOI Listing
May 2022

Friction-Dominated Carrier Excitation and Transport Mechanism for GaN-Based Direct-Current Triboelectric Nanogenerators.

ACS Appl Mater Interfaces 2022 May 16. Epub 2022 May 16.

Center on Nanoenergy Research, Research Center for Optoelectronic Materials and Devices,, School of Physical Science & Technology, Guangxi University, Nanning 530004 China.

The semiconductor triboelectric nanogenerator (TENG) based on the tribovoltaic effect has the characteristics of direct current and high current density, but the energy transfer and conversion mechanism is not completely clear. Here, a series of gallium nitride (GaN)-based semiconductor direct-current TENGs (SDC-TENGs) are investigated for clarifying the carrier excitation and transport mechanism. During the friction process, the external output current always flows from GaN to silicon or aluminum, regardless of the direction of the built-in electric field, because of the semiconductor types. These results reveal that the carrier transport direction is dominated by the interfacial electric field formed by triboelectrification, which is also verified under different bias voltages. Moreover, the characteristics dependent on the frictional force have been systematically investigated under different normal forces and frictional modes. The open-circuit voltage and short-circuit current of SDC-TENG are both increased with a larger frictional force, which shows that the more severe friction results in both a larger interface electric field and more excited carriers. The maximum voltage can reach 25 V for lighting up a series of LEDs, which is enhanced by four times compared to the cutting-edge reported SDC-TENGs. This work has clarified the friction-dominated carrier excitation and transport mechanism for the tribovoltaic effect, which demonstrates the great potential of semiconductor materials for frictional energy recovery and utilization.
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http://dx.doi.org/10.1021/acsami.2c03853DOI Listing
May 2022

An Assessment of China's Digital Trade Development and Influencing Factors.

Front Psychol 2022 26;13:837885. Epub 2022 Apr 26.

College of Science and Technology, Ningbo University, Cixi, China.

The pattern and scale of commerce worldwide have been greatly transformed by the Fourth Industrial Revolution and technological advancement; digital trade has become the primary form of trade in the digital economy. On the basis of information network infrastructure, information technology level, digital industrialization level, and industrial digitalization level, this study establishes a comprehensive assessment system that applies an entropy-TOPSIS model to evaluate digital trade development level in China. The results indicate that digital trade in China was steadily growing between 2010 and 2019. A principal component analysis is conducted to identify factors affecting the digital trade development level in China. The analysis results suggest that Internet development, population income, industrial structure, payment convenience level, fixed asset investment, online transaction scale, and economic development all have positive effects on the digital trade development level in China, with payment convenience level having the greatest influence. By contrast, state intervention and degree of dependence on foreign trade have a negative effect on digital trade development.
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http://dx.doi.org/10.3389/fpsyg.2022.837885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087175PMC
April 2022

Recent progress in microwave-assisted preparations of 2D materials and catalysis applications.

Nanotechnology 2022 Jun 7;33(34). Epub 2022 Jun 7.

State Key Laboratory of Metal Matrix Composites, Department of Material Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

On the urgency of metal-free catalysts, two-dimensional materials (2DMs) have caused extensive researches because of distinctive optical and electronic properties. In the last decade, microwave methods have emerged in rapid and effective preparations of 2DMs for catalysis. Microwave heating offers several advantages namely direct, fast, selective heating and uniform reaction temperature compared to conventional heating methods, thus bringing about high-yield and high-purity products in minutes or even seconds. This review summarizes recent advances in microwave-assisted preparations of 2DMs-based catalysts and their state-of-the-art catalytic performances. Microwave heating mechanisms are briefly introduced mainly focusing on microwave-matter interactions, which can guide the choice of precursors, liquid media, substrates, auxiliaries and experiment parameters during microwave radiation. We especially provide a detailed insight into various microwave-assisted procedures, classified as exfoliation, synthesis, doping, modification and construction towards different 2DMs nanomaterials. We also discuss how microwave affects the synthetic composition and microstructure of 2DMs-based catalysts, thereby deeply influencing their optical and electronic properties and the catalytic performances. Finally, advantages, challenges and prospects of microwave-assisted approaches for 2DMs nanomaterials are summarized to inspire the effective and large-scale fabrication of novel 2DMs-based catalysts.
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http://dx.doi.org/10.1088/1361-6528/ac6c97DOI Listing
June 2022

Strategy of Developing Oral Vaccine Candidates Against Co-infection of Porcine Diarrhea Viruses Based on a Delivery System.

Front Microbiol 2022 4;13:872550. Epub 2022 Apr 4.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

The number of co-infections with multiple porcine diarrhea viruses has increased in recent years. Inducing mucosal immunity through oral immunization is an effective approach for controlling these pathogens. To generate a multi-pathogen vaccine against viral co-infection, we employed the vector platform, which was previously used to generate potent candidate vaccines against various diseases. Two strategies were used to test the protective efficiency of recombinant against multiple diarrhea viruses. First, we used a mixture of recombinant separately expressing antigens of transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), and porcine rotavirus (PoRV). Next, we used a recombinant expressing an antigen fusion protein of the above viruses. Twenty-four newborn piglets were divided into three groups and orally immunized with a mixture of recombinant , recombinant expressing the antigen fusion protein, or sterile phosphate-buffered saline daily for seven consecutive days after birth. After immunization, the piglets were randomly selected from each group for oral administration of PEDV, and these piglets were then cohabited with piglets without PEDV infection for 7 days. The protective effect against PEDV was evaluated based on clinical symptoms, viral shedding, and intestinal pathological damage. Piglets immunized with recombinant showed specific mucosal and humoral immune responses to the three viruses and were protected against severe diarrhea and intestinal pathology. Our results highlight the potential of an oral multi-pathogen vaccine based on to prevent transmission and limit the severity of viral co-infection.
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http://dx.doi.org/10.3389/fmicb.2022.872550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014262PMC
April 2022

Corybungines A-K: Isoquinoline alkaloids from Corydalis bungeana with dopamine D2 receptor activity.

Phytochemistry 2022 Jul 14;199:113209. Epub 2022 Apr 14.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People's Republic of China; Jiangxi Provincial Key Laboratory for Pharmacodynamic Material Basis of Traditional Chinese Medicine, Ganjiang Chinese Medicine Innovation Center, Nanchang, 330000, People's Republic of China.

Eleven undescribed isoquinoline alkaloids corybungines A-K including a protoberberine-type alkaloid, an isoquinoline alkaloid with a unique 6-norprotoberberine skeleton, one 13,14-seco-protoberberine-type alkaloid, two 1a,14-seco-protoberberine-type alkaloids with a 4-(hydroxymethyl)phenoxy moiety and six aporphine alkaloids, together with seven known alkaloids, have been isolated from the whole herb extract of Corydalis bungeana Turcz. Their structures and absolute configurations were elucidated based on an analysis of spectroscopic data and electronic circular dichroism (ECD) spectra. (R)-stephanine displayed high antagonistic activity against the dopamine D2 receptor with an IC value of 0.85 ± 0.09 μM in CHO-D2 cells. Additionally, corybungines D, F, H, (R)-roemerine, (R)-vireakine and (R)-tuduranine showed moderate D2 antagonism (IC 5.20-26.07 μM). The preliminary structure-activity relationships (SARs) of aporphine alkaloids were discussed.
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http://dx.doi.org/10.1016/j.phytochem.2022.113209DOI Listing
July 2022

Establishment of a new cell line of canine inflammatory mammary cancer: IMC-118.

Vet Comp Oncol 2022 Apr 15. Epub 2022 Apr 15.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Canine inflammatory mammary cancer (IMC) has long been regarded as an attractive animal model for research into human inflammatory breast cancer (IBC), Although some canine mammary tumour cell lines corresponding to human mammary cancer cell lines have been established, there is still a need to supplement the canine mammary tumour cell bank. The goal of this study was to create a new type of IMC cell line. The primary tumour, IMC-118, was identified as IMC by pathology examination. Immunohistochemistry analysis revealed negative immunoreactivity to oestrogen receptor (ER), but positive immunoreactivity to progesterone receptor (PR) and human epidermal growth factor receptor-2 (HER-2). Immunofluorescence (IF) analysis revealed that the IMC-118 cell line from this primary tumour was negative for ER but positive for PR and HER-2, and was also positive for epithelial and mesenchymal cell markers. This cell line was cultured stably for more than 50 passages and grew well after cryopreservation. In vivo, tumour masses and metastases in the lungs were discovered after inoculating the IMC-118 cells into the nude mice model. As a result, a novel canine IMC cell line, IMC-118, was effectively established, and could be employed as a promising model for immunotherapy and epithelial-mesenchymal transition mechanism of IMC research in both dogs and humans.
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http://dx.doi.org/10.1111/vco.12822DOI Listing
April 2022

SaliVISION: a rapid saliva-based COVID-19 screening and diagnostic test with high sensitivity and specificity.

Sci Rep 2022 04 6;12(1):5729. Epub 2022 Apr 6.

Department of Pathology, Yale School of Medicine, Yale University, New Haven, CT, USA.

The Coronavirus disease 2019 (COVID-19) pandemic-caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)- has posed a global threat and presented with it a multitude of economic and public-health challenges. Establishing a reliable means of readily available, rapid diagnostic testing is of paramount importance in halting the spread of COVID-19, as governments continue to ease lockdown restrictions. The current standard for laboratory testing utilizes reverse transcription quantitative polymerase chain reaction (RT-qPCR); however, this method presents clear limitations in requiring a longer run-time as well as reduced on-site testing capability. Therefore, we investigated the feasibility of a reverse transcription looped-mediated isothermal amplification (RT-LAMP)-based model of rapid COVID-19 diagnostic testing which allows for less invasive sample collection, named SaliVISION. This novel, two-step, RT-LAMP assay utilizes a customized multiplex primer set specifically targeting SARS-CoV-2 and a visual report system that is ready to interpret within 40 min from the start of sample processing and does not require a BSL-2 level testing environment or special laboratory equipment. When compared to the SalivaDirect and Thermo Fisher Scientific TaqPath RT-qPCR testing platforms, the respective sensitivities of the SaliVISION assay are 94.29% and 98.28% while assay specificity was 100% when compared to either testing platform. Our data illustrate a robust, rapid diagnostic assay in our novel RT-LAMP test design, with potential for greater testing throughput than is currently available through laboratory testing and increased on-site testing capability.
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http://dx.doi.org/10.1038/s41598-022-09718-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986854PMC
April 2022

XGboost Prediction Model Based on 3.0T Diffusion Kurtosis Imaging Improves the Diagnostic Accuracy of MRI BiRADS 4 Masses.

Front Oncol 2022 17;12:833680. Epub 2022 Mar 17.

Radiology Department, Second Affiliated Hospital of Shantou University Medical College, Shantou, China.

Background: The malignant probability of MRI BiRADS 4 breast lesions ranges from 2% to 95%, leading to unnecessary biopsies. The purpose of this study was to construct an optimal XGboost prediction model through a combination of DKI independently or jointly with other MR imaging features and clinical characterization, which was expected to reduce false positive rate of MRI BiRADS 4 masses and improve the diagnosis efficiency of breast cancer.

Methods: 120 patients with 158 breast lesions were enrolled. DKI, Diffusion-weighted Imaging (DWI), Proton Magnetic Resonance Spectroscopy (H-MRS) and Dynamic Contrast-Enhanced MRI (DCE-MRI) were performed on a 3.0-T scanner. Wilcoxon signed-rank test and χ2 test were used to compare patient's clinical characteristics, mean kurtosis (MK), mean diffusivity (MD), apparent diffusion coefficient (ADC), total choline (tCho) peak, extravascular extracellular volume fraction (V), flux rate constant (K) and volume transfer constant (K). ROC curve analysis was used to analyze the diagnostic performances of the imaging parameters. Spearman correlation analysis was performed to evaluate the associations of imaging parameters with prognostic factors and breast cancer molecular subtypes. The Least Absolute Shrinkage and Selectionator operator (lasso) and the area under the curve (AUC) of imaging parameters were used to select discriminative features for differentiating the breast benign lesions from malignant ones. Finally, an XGboost prediction model was constructed based on the discriminative features and its diagnostic efficiency was verified in BiRADS 4 masses.

Results: MK derived from DKI performed better for differentiating between malignant and benign lesions than ADC, MD, tCho, K and K (). Also, MK was shown to be more strongly correlated with histological grade, Ki-67 expression and lymph node status. MD, MK, age, shape and menstrual status were selected to be the optimized feature subsets to construct an XGboost model, which exhibited superior diagnostic ability for breast cancer characterization and an improved evaluation of suspicious breast tumors in MRI BiRADS 4.

Conclusions: DKI is promising for breast cancer diagnosis and prognostic factor assessment. An optimized XGboost model that included DKI, age, shape and menstrual status is effective in improving the diagnostic accuracy of BiRADS 4 masses.
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http://dx.doi.org/10.3389/fonc.2022.833680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8968064PMC
March 2022

Cognitive function and elderly macronutrient intakes from rural diets in Qingdao, China.

Asia Pac J Clin Nutr 2022 Mar;31(1):118-127

Institute of Nutrition & Health, Qingdao University, Qingdao, China.

Background And Objectives: Energy provided by macronutrients plays a key role in healthy aging. This study therefore explored the association between macronutrients and cognitive function in elderly populations in rural areas of Qingdao, China.

Methods And Study Design: This study included 1,504 participants over the age of 65 recruited from Licha Town, Qingdao City, China. Dietary intake was measured using the Food Frequency Questionnaire, and cognitive function was assessed using the Mini-Mental State Examination. Logistic regression models were used to evaluate the association between dietary macronutrient intake and cognitive function. In addition, restricted cubic bars were applied to determine the dose-response relationship between macronutrient ratios and cognitive performance.

Results: A total of 877 adults over the age of 65 were included. After adjusting the weighted multiple variables, significant positive associations were revealed between protein and moderate carbohydrate intake and cognitive ability, but a negative association between fat intake and cognitive performance was identified. After calculating the daily energy supply ratio, similar associations were revealed between fat and protein intake and cognitive function. Furthermore, the ratio of proteins to carbohydrates had a U-shaped relationship with cognitive function (pnonlinearity=0.674), whereas the ratio of proteins to fats was L-shaped with lower cognitive function (pnonlinearity<0.001). Compared with the lowest quartile of the ratio of protein to fat intake, the weighted adjusted OR (95% CI) of the highest quartile was 0.509 (0.314, 0.827) for low cognitive performance.

Conclusions: With an adequate carbohydrate supply, appropriately increasing dietary protein intake and reducing fat intake might benefit the cognitive function of elders in rural areas.
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http://dx.doi.org/10.6133/apjcn.202203_31(1).0013DOI Listing
March 2022

[Risk assessment of lead and cadmium exposure of Nanjing residents through vegetables and edible fungus from 2014 to 2018].

Wei Sheng Yan Jiu 2022 Jan;51(1):118-127

Nanjing Center for Disease Control and Prevention, Nanjing 210003, China.

Objective: To evaluate the potential risk of lead and cadmium through vegetables and edible fungus in Nanjing City from 2014 to 2018.

Methods: Based on the survey of dietary consumption of residents in Nanjing City, combining with the concentration data of lead and cadmium in vegetables and edible fungus, using @Risk software to simulate and analyze the cumulative dietary exposure in a probabilistic way, and compared with the health guidance value or benchmark dose(BMD) to assess the potential health risks of lead and cadmium for Nanjing residents.

Results: The average exposure of lead and cadmium was 0.237 and 0.090 μg/kg, the exposure of cadmium in the general population were lower than the corresponding health guidance value, meanwhile the margin of exposure(MOE) for lead was higher than 1 in 18-49 years old group and over 50 years old group respectively, except for the high-end exposure(P95) in 6-17 years group the MOE for lead was 0.9. The pollution level and the consumption of leafy vegetables, edible fungus and artemisia selengensis were the main factors affecting the exposure of lead and cadmium through vegetables and edible fungus.

Conclusion: The exposure to lead and cadmium through vegetables is at a relatively safe level, but more attention should be paid to the risk of lead and cadmium exposure in 6-17 years old and over 50 years old group of high-end exposure population.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2022.01.020DOI Listing
January 2022

Efficacy Assessment of Phage Therapy in Treating -Induced Mastitis in Mice.

Viruses 2022 03 16;14(3). Epub 2022 Mar 16.

Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, China.

The primary aim of this study was to evaluate the efficacy of phage against mastitis induced by drug-resistant in a mouse model. In this study, five phages-4086-1, 4086-2, 4086-3, 4086-4, and 4086-6-were isolated from milk samples secreted by mastitis cows. Transmission electron microscopy showed that all the five phages had icosahedral heads and short non-contractile tails, which are typical characteristics of the family Podoviridae. All these phages were species-specific against . The one-step growth curve showed a short latency period (10-20 min) and high burst size (up to 400 PFU/infected cell). To evaluate the effectiveness of the phage 4086-1 in the treatment against mastitis, a mouse model of mastitis was challenged with drug-resistant . The results showed the proliferation of in the mammary glands was significantly inhibited after treating by phage 4086-1. The concentrations of TNF-α and IL-6 decreased significantly, which demonstrated the phages could effectively alleviate the inflammatory responses. Furthermore, the histopathological analysis showed that inflammatory infiltration in the mammary glands was significantly reduced. These results demonstrate that phage may be a promising alternative therapy against mastitis caused by drug-resistant .
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http://dx.doi.org/10.3390/v14030620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954217PMC
March 2022

Ginsenoside Rg3 determination using an electro-synthesized molecularly imprinted polymer on MWCNT-TiCT nanocomposite modified electrode.

Talanta 2022 Jun 15;243:123391. Epub 2022 Mar 15.

Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety, College of Life Sciences, Shandong Normal University, Jinan, Shandong, 250014, China. Electronic address:

Rare ginsenoside Rg3 is a main active ingredient in ginseng, which is more easily absorbed by human body and plays its role. Determination of Rg3 in edible and medicinal samples is a key factor for quality evaluation and effective monitoring of adulteration. In this study, an electrochemical sensor was developed based on molecularly imprinted polymer (MIP) and nanomaterial amplification. MIP was achieved by electro-polymerization with Rg3 as the template molecule and the functional monomer was o-phenylenediamine. Multi-walled carbon nanotubes (MWCNT) and titanium carbide (TiCT) nanocomposites formed an excellent porous structure, which exhibited the advantages of increasing the specific surface area and electrical conductivity. The prepared sensor presented a great linear relation of Rg3 in the range of 10-2000 μg mL with a limit of detection (LOD) of 0.34 μg mL. Importantly, the sensor was successfully utilized to detect ginsenoside Rg3 in different real samples.
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http://dx.doi.org/10.1016/j.talanta.2022.123391DOI Listing
June 2022
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