Publications by authors named "Han Wang"

1,445 Publications

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A Novel Management Platform Based on Personalized Home Care Pathways for Medicine Management and Rehabilitation of Persons With Parkinson's Disease-Requirements and Implementation Plan of the Care-PD Program.

Front Neurol 2021 25;12:672208. Epub 2021 May 25.

School of Kinesiology, Shanghai University of Sport, Shanghai, China.

As the percentage of the aging population increases, the incidence of Parkinson's disease (PD) in China is increasing year by year. PD is both a public health and social problem facing the government and society as a whole. Persons with PD need reasonable medication management and rehabilitation strategies after a clear diagnosis. A proper home care plan can effectively slow the progression of PD. However, people with PD lack an effective way to manage their illnesses and cannot achieve the recommended clinical path in a family environment. Medication management, condition monitoring, and rehabilitation training are important components of the home care plan for PD. Persons with PD require strategies that delay the development of the disease and to adhere to treatment, which would contribute to improving their quality of life. Thus, we developed a small program called Care-PD to build a medicine management and service platform for PD. The development of Care-PD is a multi-dimensional model designed for PD, which is funded by the National Key R&D Program of China (No. 2018YFC1314700), and includes services such as medication management, symptom monitoring, professional counseling, home life, and community communication. Care-PD can become a key technology that increases the compliance of persons with PD with home care plans and improve measures to control the disease. In this article, we describe the medication management and services for PD based on the Care-PD program and its structure. The small program will improve the adverse conditions faced by persons with PD by combining the latest technology and clinical approaches. Meanwhile, we describe a verification strategy to evaluate the effectiveness of the Care-PD program as a comprehensive management strategy for PD.
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http://dx.doi.org/10.3389/fneur.2021.672208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186830PMC
May 2021

Identification of 27 abnormalities from multi-lead ECG signals: an ensembled SE_ResNet framework with sign loss function.

Physiol Meas 2021 Jun 7. Epub 2021 Jun 7.

Saw Swee Hock School of Public Health, National University Health System, National University of Singapore, Singapore, SINGAPORE.

Objective: Cardiovascular disease is a major threat to health and one of the primary causes of death globally. The 12-lead ECG is a cheap and commonly accessible tool to identify cardiac abnormalities. Early and accurate diagnosis will allow early treatment and intervention to prevent severe complications of cardiovascular disease. Our objective is to develop an algorithm that automatically identifies 27 ECG abnormalities from 12-lead ECG databases.

Approach: Firstly, a series of pre-processing methods were proposed and applied on various data sources in order to mitigate the problem of data divergence. Secondly, we ensembled two SE_ResNet models and one rule-based model to enhance the performance of various ECG abnormalities' classification. Thirdly, we introduce a Sign Loss to tackle the problem of class imbalance, and thus improve the model's generalisability.

Main Results: In the PhysioNet/Computing in Cardiology Challenge 2020, our proposed approach achieved a challenge validation score of 0.682, and a full test score of 0.514, placed us 3rd out of 40 in the official ranking.

Significance: We proposed an accurate and robust predictive framework that combines deep neural networks and clinical knowledge to automatically classify multiple ECG abnormalities. Our framework is able to identify 27 ECG abnormalities from multi-lead ECG signals regardless of discrepancies in data sources and the imbalance of data labelling. We trained our framework on five datasets and validated it on six datasets from various countries. The outstanding performance demonstrate the effectiveness of our proposed framework.
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http://dx.doi.org/10.1088/1361-6579/ac08e6DOI Listing
June 2021

Microbial community and metabolic responses to electrical field intensity for alleviation of ammonia inhibition in an integrated bioelectrochemical system (BES).

Bioresour Technol 2021 May 27;336:125332. Epub 2021 May 27.

School of Chemical Engineering, Pusan National University, 63 Busandeahak-ro, Geumjeong-Gu, Busan 46241, Republic of Korea.

Bioelectrochemical system (BES) is a promising solution for mitigation of ammonia inhibition in anaerobic digestion (AD) process. However, the effect of electric field intensity on microbial community changes and metabolic function prediction during the alleviation of ammonia inhibition are still missing. The results of the current study represented that the improvement of ammonia removal (20.6%) and methane production (14.6%) could both be achieved at 0.2 V while higher voltages led to reductions of methane production (more than 48.9%) compared with the control. Moreover, hydrogenotrophic methanogens (Methanobacterium) seemed to be more robust to high voltages compared with aceticlastic methanogens (Methanosaeta). Additionally, bacteria for hydrolysis and acidogenesis (Rikenellaceae and Soehngenia) were found vulnerable to external electric field intensity. Furthermore, abundances changes of metabolic pathways demonstrated that the degradation of carbohydrates, lipids and proteins during all steps (hydrolysis, acidogenesis, acetogenesis and methanogenesis) of AD process could be affected by different applied voltages.
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http://dx.doi.org/10.1016/j.biortech.2021.125332DOI Listing
May 2021

Determination of quinolones in environmental water and fish by magnetic metal organic frameworks based magnetic solid-phase extraction followed by high-performance liquid chromatography-tandem mass spectrometry.

J Chromatogr A 2021 May 25;1651:462286. Epub 2021 May 25.

Wuhan Customs District of China, Wuhan 430020, China.

The widespread use of quinolones has become an increasing global public health threat. In this study, IRMOF-3 coated SiO/FeO were prepared via a facile room-temperature method. The prepared IRMOF-3 coated SiO/FeO was used as a sorbent for magnetic solid phase extraction, and then combined with high-performance liquid chromatography-tandem mass spectrometry for the determination of 10 quinolines. The extraction conditions of magnetic solid phase extraction were studied in detail, and the optimal conditions were established. Under the optimal experimental conditions, the limits of quantification of 10 quinolones were in the range of 0.005-0.01 μg L, the relative standard deviations were 6.58-10.6% (n=7), the enrichment factors were 21.0-23.8 for water samples. The limits of quantification of 10 quinolones were in the range of 0.10-0.20 μg kg, the relative standard deviations were 5.95-14.5% (n=7), the enrichment factors were 1.08-1.24 for fish samples. The proposed method was applied for the determination of 10 quinolones in river water, aquacultural water and a fish sample, and enrofloxacin and ciprofloxacin were found in the fish sample.
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http://dx.doi.org/10.1016/j.chroma.2021.462286DOI Listing
May 2021

Transcriptional regulation of the mannitol phosphotransferase system operon by the ferric uptake regulator (Fur) in Vibrio cholerae El Tor Serogroup O1.

Res Microbiol 2021 Jun 2:103848. Epub 2021 Jun 2.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. Electronic address:

The phosphoenolpyruvate (PEP): carbohydrate phosphotransferase system (PTS) allows bacteria to use various carbohydrates as energy resources including mannitol. The mannitol-specific PTS transporter in Vibrio cholerae is encoded by the mtlADR operon. Expression of the mtl operon has been shown to be strictly regulated by CRP, MtlS, and MtlR. In the present study, we investigated the regulation of mtlADR by the ferric uptake regulator (Fur). The results showed that Fur binds to the promoter-proximal DNA region of mtlADR to repress its transcription independent of iron, in mannitol-containing growth medium. The capacity for mannitol fermentation was significantly increased in Δfur relative to that of WT for normal and iron-replete growth media. The level of organic acids produced by Δfur was significantly enhanced relative to that produced by the WT strain in the normal and iron-replete media but not in an iron-starved medium. The results provided for a deeper understanding of the regulation of mtlADR in V. cholerae.
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http://dx.doi.org/10.1016/j.resmic.2021.103848DOI Listing
June 2021

Effect of poly (lactic acid) porous membrane prepared via phase inversion induced by water droplets on 3 T3 cell behavior.

Int J Biol Macromol 2021 Jun 1. Epub 2021 Jun 1.

College of material science and engineering, Wuhan Textile University, Wuhan 430200, PR China; Key Laboratory of Green Processing and Functional New Textile Materials of Ministry of Education, Wuhan Textile University, Wuhan 430200, PR China. Electronic address:

Phase inversion induced by water droplets has garnered attention in the field of polymer science as a novel method for preparing porous membranes. This study investigates the effect of the porous structure of poly (lactic acid) (PLA) membranes prepared through phase inversion induced by water droplets at four different temperatures (25, 50, 75, and 100 °C) on the morphology and proliferation of 3 T3 cells. The surface properties of the PLA porous membrane, including pore size, pore size distribution, surface roughness, surface hydrophilicity, and cytocompatibility with 3 T3 cells, were evaluated. The results indicated that the synthesized PLA membrane had two surfaces with different structures. The upper surface in contact with the water droplets during preparation contained uniformly distributed micropores, whereas the bottom surface was smooth and composed of small particles in contacted with the mold. The upper surface showed high cytocompatibility with 3 T3 cells, and the 3 T3 cells migrated and grew within the pores at 25 °C. In contrast, the bottom surface exhibited low biocompatibility with the 3 T3 cells. Our study has wide-ranging implications and will improve the fabrication and implementation of 3D cultured scaffolds with excellent cytocompatibility.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.197DOI Listing
June 2021

Response of antioxidant defense to oxidative stress induced by HO and NO in anammox bacteria.

Chemosphere 2021 May 28;282:131008. Epub 2021 May 28.

State Key Laboratory of Pollution Control and Resources Reuse, Shanghai Institute of Pollution Control and Ecological Security, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai, 200092, PR China. Electronic address:

Exposure to the stressful environment results in excessive generation of reactive oxygen species (ROS) or reactive nitrogen species (RNS) in anaerobes, which causes deterioration of microbial activities in biological wastewater treatment systems. Although the genes involved in oxidative stress defense have been primarily identified in the genome of Candidatus Kuenenia stuttgartiensis (a typical anammox species), their function is still not verified. Therefore, the expression of putative antioxidation genes kat, sor, and sod in anammox bacteria was studied by in situ transcription and function validated by heterologous expression under the typical ROS (HO) and RNS (NO) stress. After HO and NO additions, the genes involved in the anammox central metabolism (nirS, hzsB, and hdh) were immediately down expressed consistent with the decreased anammox activity. However, the expression of putative antioxidation gene kat did not rise when exposed to HO; whereas, its encoding protein KAT enhanced the antioxidant actively of anammox bacteria by HO decomposition like the oxidoreductase enzyme catalase. The sod and sor gene were upregulated with NO treatment, and SOD and SOR can combine with NO and decrease its concentration efficiently. These confirmed the important role of kat, sod, and sor as ROS/RNS scavengers in anammox bacteria, with which anammox bacteria protect themselves when they are exposed to the stressful environment. These verified functional enzymes provide directions for the future regulation of anammox systems, which helps to mitigate the inhibitory effect of the stressful environment on anammox bacteria.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131008DOI Listing
May 2021

Bioinspired Cellulose Foam with Excellent Water Wicking and Flux Properties Prepared Using Ice Template.

J Phys Chem B 2021 Jun 1;125(22):5853-5861. Epub 2021 Jun 1.

College of Materials Science and Engineering, Wuhan Textile University, Wuhan, China 430200.

The application and functionalization of cellulose has been attracting increased attention in academic and industrial studies because of its wide range of sources, short renewable cycle, and low environmental impact. In order to enhance the application field of cellulose and decrease the environmental pollution for organic solvent associated with its preparation, cellulose foam with a vertically hierarchically porous structure similar to wood was designed and fabricated successfully from a cellulose aqueous solution using an ice templated in this study. The cellulose foam prepared using a 3 wt % concentration possessed a uniform vertical hierarchically porous structure, which could provide a pathway for the flow of water or air based on the capillary effect. The highest water wicking rate and flux were 7.8184 mm·s and 29.49 mL·min·g, respectively, for the porous foam prepared using a 3 wt % concentration. The mechanical testing experiment showed that the porous structure did not reduce the amount of stress that the sample could endure before being damaged. The compression strength increased with increasing cellulose concentration in solution. Therefore, the hierarchical structure formed in the prepared cellulose foam effectively improved the water flux behavior and provided a structural basis for future applications of cellulose scaffolds.
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http://dx.doi.org/10.1021/acs.jpcb.1c01416DOI Listing
June 2021

Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non-coding RNAs in the spinal cord of mice as determined by sequencing analysis.

Exp Ther Med 2021 Jul 18;22(1):775. Epub 2021 May 18.

Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510000, P.R. China.

Diabetic neuropathic pain (DNP) is one of the most serious complications of diabetes. Patients with DNP always exhibit spontaneous and stimulus-evoked pain. However, the pathogenesis of DNP remains to be fully elucidated. Non-coding RNAs (ncRNAs) serve important roles in several cellular processes and dysregulated expression may result in the development of several diseases, including DNP. Although ncRNAs have been suggested to be involved in the pathogenesis of DNP, their precise roles remain to be determined. In the present study, sequencing analysis was used to investigate the expression patterns of coding genes, microRNAs (miRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs) in the spinal cord of mice with streptozotocin (STZ)-induced DNP. A total of 30 mRNAs, 148 miRNAs, 9 lncRNAs and 135 circRNAs exhibited significantly dysregulated expression 42 days after STZ injection. Functional enrichment analysis indicated that protein digestion and absorption pathways were the most significantly affected pathways of the differentially expressed (DE) mRNAs. The Rap1 signaling pathway, human T-lymphotropic virus-I infection and the MAPK signaling pathway were the three most significant pathways of the DE miRNAs. A total of 2,118 distinct circRNAs were identified and the length of the majority of the circRNAs was <1,000 nucleotides (nt) (1,552 circRNAs were >1,000 nt) with a median length of 620 nt. In the present study, the expression characteristics of coding genes, miRNAs, lncRNAs and circRNAs in DNP mice were determined; it paves the road for further studies on the mechanisms associated with DNP and potentially facilitates the discovery of novel ncRNAs for therapeutic targeting in the management of DNP.
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http://dx.doi.org/10.3892/etm.2021.10207DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145263PMC
July 2021

Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy.

Diabetes Metab Syndr Obes 2021 13;14:2133-2143. Epub 2021 May 13.

Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250012, People's Republic of China.

Introduction: Recent studies have shown that YAP is closely related to the pathological process of cardiovascular diseases. But the role of YAP in cardiac injury of diabetic cardiomyopathy (DCM) is still unclear.

Methods: Diabetic cardiomyopathy rat model was established and divided into control group, DCM group, LV-SC-shRNA group and LV-YAP-shRNA group. LV-SC-shRNA group and LV-YAP-shRNA group were injected with lentivirus expressing SC-shRNA and YAP-shRNA via tail vein, respectively. Primary rat cardiac fibroblasts (CFs) were stimulated with high concentration of glucose and treated with recombinant lentivirus expressing either SC-shRNA or YAP-shRNA to observe the expression of CTGF and fibronectin, so as to observe the effect of inhibiting YAP on the pathogenesis of DCM.

Results: Compared with control group, high glucose markedly increased YAP mRNA and protein expression in DCM and CFs. Inhibition of YAP decreased myocardial fibrosis and improved cardiac function in the DCM model and decreased the expression of CTGF and fibronectin in CFs. The result suggested that YAP plays a key role in the pathological progression of DCM, and the underlying mechanisms may be associated with TEAD and CTGF.

Discussion: We found that the expression of YAP was increased both in vivo and in vitro, suggesting that YAP is closely related to DCM, and YAP knockdown can reduce myocardial fibrosis in rat model of DCM by reducing the expression of PAI-1, collagen I, collagen III, CTGF and profilin, as well as the expression of CTGF and fibronectin in CFs. This study revealed that YAP plays an important role in the pathological process of diabetic cardiomyopathy, and down-regulation of YAP expression may provide a new therapeutic target for DCM.
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http://dx.doi.org/10.2147/DMSO.S302466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128377PMC
May 2021

The Biobanque québécoise de la COVID-19 (BQC19)-A cohort to prospectively study the clinical and biological determinants of COVID-19 clinical trajectories.

PLoS One 2021 19;16(5):e0245031. Epub 2021 May 19.

Department of Human Genetics, Faculty of Medicine, McGill University, Montreal, QC, Canada.

SARS-CoV-2 infection causing the novel coronavirus disease 2019 (COVID-19) has been responsible for more than 2.8 million deaths and nearly 125 million infections worldwide as of March 2021. In March 2020, the World Health Organization determined that the COVID-19 outbreak is a global pandemic. The urgency and magnitude of this pandemic demanded immediate action and coordination between local, regional, national, and international actors. In that mission, researchers require access to high-quality biological materials and data from SARS-CoV-2 infected and uninfected patients, covering the spectrum of disease manifestations. The "Biobanque québécoise de la COVID-19" (BQC19) is a pan-provincial initiative undertaken in Québec, Canada to enable the collection, storage and sharing of samples and data related to the COVID-19 crisis. As a disease-oriented biobank based on high-quality biosamples and clinical data of hospitalized and non-hospitalized SARS-CoV-2 PCR positive and negative individuals. The BQC19 follows a legal and ethical management framework approved by local health authorities. The biosamples include plasma, serum, peripheral blood mononuclear cells and DNA and RNA isolated from whole blood. In addition to the clinical variables, BQC19 will provide in-depth analytical data derived from the biosamples including whole genome and transcriptome sequencing, proteome and metabolome analyses, multiplex measurements of key circulating markers as well as anti-SARS-CoV-2 antibody responses. BQC19 will provide the scientific and medical communities access to data and samples to better understand, manage and ultimately limit, the impact of COVID-19. In this paper we present BQC19, describe the process according to which it is governed and organized, and address opportunities for future research collaborations. BQC19 aims to be a part of a global communal effort addressing the challenges of COVID-19.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245031PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133500PMC
May 2021

The absorption of glycosaminoglycans of different molecular weight obtained from : an and study.

Food Funct 2021 May 18. Epub 2021 May 18.

Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong Province 266003, People's Republic of China. and Laboratory of Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology, Qingdao, Shandong Province 266237, People's Republic of China.

The purpose of this study was to investigate the absorption and transport of glycosaminoglycan from Apostichopus japonicus (AHG) and its depolymerized derivatives (DAHG-1, DAHG-2, DAHG-3). The AHG and depolymerized AHGs (DAHGs) were characterized by high-performance gel permeation chromatography (HPGPC), Raman spectroscopy and atomic force microscopy (AFM). The results showed that there was no significant difference of an AHG primary structure and functional groups during the depolymerization. Meanwhile, AFM observation showed that AHG and DAHGs possessed linear structures. In this study, a rapid and sensitive liquid chromatographic post-column derivatization method was used to investigate the absorption of AHG and DAHGs with the Caco-2 cell model, the M cell model and the intestinal recirculating perfusion. It was found that AHG and DAHGs can be absorbed in the intestine, and their transport involved endocytosis.
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http://dx.doi.org/10.1039/d1fo00624jDOI Listing
May 2021

Correction: Transgenic PDGF-BB/sericin hydrogel supports for cell proliferation and osteogenic differentiation.

Biomater Sci 2021 Jun;9(11):4212-4213

Biological Science Research Center, Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, 400715, People's Republic of China.

Correction for 'Transgenic PDGF-BB/sericin hydrogel supports for cell proliferation and osteogenic differentiation' by Feng Wang et al., Biomater. Sci., 2020, 8, 657-672, DOI: 10.1039/C9BM01478K.
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http://dx.doi.org/10.1039/d1bm90052hDOI Listing
June 2021

Gradient radiation breeding and culture domestication of menaquinone producing strains.

Bioprocess Biosyst Eng 2021 Jul 18;44(7):1373-1382. Epub 2021 May 18.

Key Laboratory of High Magnetic Field And Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Shushanhu Road No. 350, Hefei, 210031, Anhui, China.

By comparing the survival rate and positive mutation rate of the primary mutagenic strain and progeny mutagenic strain under different radiation doses, the results showed that the tolerance of the mutagenic strain to radiation dose increased with the increase of the mutagenic generations. We adopted an improved gradient radiation breeding strategy to improve the breeding efficiency. The strains were treated with radiation in four stages. The first stage was low energy N ion implantation (ion energy 15 keV, dose 80 × 2.6 × 10 cm). In the second stage, the energy and dose of N ion reached to 20 keV, 90 × 2.6 × 10 cm. In the third stage, Co-γ radiation (dose of 1.56 kGy) was used. In the fourth stage, the radiation dose of Co-γ increased to 1.82 kGy. After each stage of radiation, the MK (Menaquinone) precursor 1, 4-dihydroxy-2-naphthalate (DHNA) was used as the stress factor to domesticate the mutant strains. By gradually increasing the concentration of DHNA in the culture medium, the substrate tolerance of Flavobacterium sp. was effectively improved. By measuring SOD (superoxide dismutase) activity and malondialdehyde, it showed that the cell damage caused by radiation mutagenesis to the offspring mutant was less than that of the primary mutant. Changes in membrane permeability and membrane potential of the mutant strains were reflected in changes in fluorescence intensity of luciferin diacetate and rhodamine 123, which could explain the enhanced substrate tolerance of strain F-2. After gradient radiation breeding and culture acclimation, the biomass of mutant Strain F-2 was 6.59 g/L, and the MK yield was 9.59 mg/L.
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http://dx.doi.org/10.1007/s00449-021-02508-8DOI Listing
July 2021

TIM-4 is critical for KC homeostatic function in the activation and resolution of liver ischemia reperfusion injury.

Hepatology 2021 May 17. Epub 2021 May 17.

Dumont-UCLA Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, David Geffen School of Medicine at University of California-Los Angeles, Los Angeles, CA, USA.

Liver ischemia reperfusion injury (IRI) remains an unresolved clinical problem. This study dissected roles of liver resident macrophage Kupffer cells (KCs), with a functional focus on efferocytosis receptor TIM-4, in both the activation and resolution of IRI in a murine liver partial warm ischemia model. FACS results showed that TIM-4 was expressed exclusively by KCs, but not infiltrating macrophages (iMФs) in IR-livers. Anti-TIM-4 Ab depleted TIM-4 macrophages in vivo, resulting in either alleviation or deterioration of liver IRI, which was determined by the repopulation kinetics of the KC niche with CD11b macrophages. To determine KC-specific function of TIM-4, we reconstituted clodronate liposome-treated mice with exogenous WT or TIM-4 deficient KCs at either 0h or 24h post reperfusion. TIM-4 deficiency in KCs resulted in not only increases in the severity of liver IRI (at 6h post reperfusion) but also impairment of the inflammation resolution (at 7 days post reperfusion). In vitro analysis revealed that TIM-4 promoted KC efferocytosis to regulate their TLR response by upregulating IL-10 and downregulating TNF-α productions. In conclusion, TIM-4 is critical for KC homeostatic function in both the activation and resolution of liver IRI via efferocytosis.
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http://dx.doi.org/10.1002/hep.31906DOI Listing
May 2021

The association between carotenoids and subjects with overweight or obesity: a systematic review and meta-analysis.

Food Funct 2021 Jun;12(11):4768-4782

Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, 130021, P. R. China.

Background: Excess body weight, including overweight and obesity, is one of the major factors influencing human health, and plays an important role in the global burden of disease. Carotenoids serve as precursors of vitamin A-related retinoids, and are considered to have potential effects on many diseases. However, the influence of carotenoids on people with excess body weight is unclear.

Methods: This meta-analysis was conducted to assess the effects of carotenoids on overweight or obese subjects utilizing the available evidence. We searched PubMed, Medline, Cochrane Library, Web of Science and EMBASE databases up to September 2020. Random effects models were used to calculate the standard mean differences (SMDs) and odds ratios (ORs) with their 95% confidence intervals (95% CIs).

Results: A total of seven randomized controlled trials and eight observational studies met the inclusion criteria and contained 28 944 subjects and data on multiple carotenoid subgroups, including lycopene, astaxanthin, cryptoxanthin, α-carotene, and β-carotene. In all included Randomized Controlled Trial (RCT), the intervention duration was 20 days at the shortest and 16 weeks at the longest, and the range of intervention doses was 1.2-60 mg d-1. Our study found that the insufficiency of serum carotenoids was a risk factor for overweight and obesity (OR = 1.73, 95% CI [1.57, 1.91], p < 0.001). Moreover, carotenoid supplementation was significantly associated with body weight reductions (SMD = -2.34 kg, 95% CI [-3.80, -0.87] kg, p < 0.001), body mass index decrease (BMI, SMD = -0.95 kg cm-2, 95% CI [-1.88, -0.01] kg cm-2, p < 0.001) and waist circumference losses (WC, SMD = -1.84 cm, 95% CI [-3.14, -0.54]cm, p < 0.001).

Conclusion: In summary, the carotenoids show promising effects in overweight or obese subjects. Additional data from large clinical trials are needed.
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http://dx.doi.org/10.1039/d1fo00004gDOI Listing
June 2021

Genome-wide identification and expression analysis of the EXO70 gene family in grape ( L).

PeerJ 2021 21;9:e11176. Epub 2021 Apr 21.

Department of Horticulture, Gansu Agricultural University, Lanzhou, China.

EXO70 is the pivotal protein subunit of exocyst, which has a very crucial role in enhancing the shielding effect of the cell wall, resisting abiotic and hormonal stresses. This experiment aims to identify family members of the gene family in grape and predict the characteristics of this gene family, so as to lay the foundation of further exploring the mechanism of resisting abiotic and hormone stresses of . Therefore, the 'Red Globe' tube plantlet were used as materials. Bioinformatics was used to inquire VvEXO70 genes family members, gene structure, system evolution, -acting elements, subcellular and chromosomal localization, collinearity, selective pressure, codon bias and tissue expression. All of VvEXO70s had the conserved pfam03081 domain which maybe necessary for interacting with other proteins. Microarray analysis suggested that most genes expressed to varying degrees in tendrils, leaves, seeds, buds, roots and stems. Quantitative Real-Time PCR (qRT-PCR) showed that the expression levels of all genes with 5 mM salicylic acid (SA), 0.1 mM methy jasmonate (MeJA), 20% PEG6000 and 4 °C for 24 h were higher than for 12 h. With 20% PEG6000 treatment about 24 h, the relative expression of was significantly up-regulated and 361 times higher than CK. All genes' relative expression was higher at 12 h than that at 24 h after treatment with 7 mM hydrogen peroxide (HO) and 0.1 mM ethylene (ETH). In conclusion, the expression levels of 14 genes are distinguishing under these treatments, which play an important role in the regulation of anti-stress signals in grape. All of these test results provide a reference for the future research on the potential function analysis and plant breeding of genes.
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http://dx.doi.org/10.7717/peerj.11176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067907PMC
April 2021

Development of the prediction model for hypertension in patients with idiopathic inflammatory myopathies.

J Clin Hypertens (Greenwich) 2021 May 11. Epub 2021 May 11.

Department of Cardiology, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Sichuan, China.

Cardiac involvement is an important cause of morbidity and mortality in patients with idiopathic inflammatory myopathies (IIMs). Hypertension, an important cardiovascular risk factor for the general population, has a crucial role in heart involvement. However, few studies have focused on the hypertension associated with IIMs. This study aimed to develop and assess the prediction model for incident hypertension in patients with IIMs. A retrospective cohort study was performed on 362 patients with IIMs, of whom 54 (14.9%) were given a diagnosis of new-onset hypertension from January 2008 to December 2018. The predictors of hypertension in IIMs were selected by least absolute shrinkage and selection operator (LASSO) regression, multivariable logistic regression, and clinically relevance, and then these predictors were used to draw the nomogram. Discrimination, calibration and clinical usefulness of the model were evaluated using the C-index, calibration plot, and decision curve analysis, respectively. The predicting model was validated by the bootstrapping validation. The nomogram mainly included predictors such as age, diabetes mellitus, triglyceride, low-density lipoprotein-cholesterol (LDL-C), antinuclear antibodies (ANA), and smoking. This prediction model demonstrated good discrimination with a C-index of 0.754 (95%CI, 0.684 to 0.824) and good calibration. The C-index of internal validation was 0.728, and decision curve analysis demonstrated that this nomogram was clinically useful. Clinicians can use this prediction model to assess the risk of hypertension in IIMs patients, and early preventive measures should be taken to reduce the incidence of hypertension in high-risk patients.
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http://dx.doi.org/10.1111/jch.14267DOI Listing
May 2021

Presynaptic coupling by electrical synapses coordinates a rhythmic behavior by synchronizing the activities of a neuron pair.

Proc Natl Acad Sci U S A 2021 May;118(20)

Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, CA 90033;

Electrical synapses are specialized structures that mediate the flow of electrical currents between neurons and have well known roles in synchronizing the activities of neuronal populations, both by mediating the current transfer from more active to less active neurons and by shunting currents from active neurons to their less active neighbors. However, how these positive and negative functions of electrical synapses are coordinated to shape rhythmic synaptic outputs and behavior is not well understood. Here, using a combination of genetics, behavioral analysis, and live calcium imaging in , we show that electrical synapses formed by the gap junction protein INX-1/innexin couple the presynaptic terminals of a pair of motor neurons (AVL and DVB) to synchronize their activation in response to a pacemaker signal. Live calcium imaging reveals that /innexin mutations lead to asynchronous activation of AVL and DVB, due, in part, to loss of AVL-mediated activation of DVB by the pacemaker. In addition, loss of leads to the ectopic activation of DVB at inappropriate times during the cycle through the activation of the L-type voltage-gated calcium channel EGL-19. We propose that electrical synapses between AVL and DVB presynaptic terminals function to ensure the precise and robust execution of a specific step in a rhythmic behavior by both synchronizing the activities of presynaptic terminals in response to pacemaker signaling and by inhibiting their activation in between cycles when pacemaker signaling is low.
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http://dx.doi.org/10.1073/pnas.2022599118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157971PMC
May 2021

Quantitative analysis of 20 fentanyl analogues by modified QuEChERS-LC-MS/MS in health products and transdermal patches.

J Pharm Biomed Anal 2021 Jul 27;201:114100. Epub 2021 Apr 27.

Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, PR China. Electronic address:

The spreading of narcotics especially illicit novel psychoactive substances is a continuing problem in recent years. In response to reduce the morbidity and crime related to fentanyl analogues, the accurate measurement of fentanyl analogues concentrations is significantly important in the analytical laboratories for customs checks and clinical application. In this work, ethyl acetate was selected as extraction solvent, 50 mg of PSA, 100 mg of C18, and 10 mg of GCB were optimized for purification. A modified QuEChERS extraction method followed by high performance liquid chromatography-tandem mass spectrometry with the mode of multiple reaction monitoring has been developed for the simultaneous determination of 20 fentanyl analogues in collagen peptides, slimming capsules and fentanyl transdermal patches. The limits of detection (LODs) varied from 0.004 to 0.02 μg L with relative standard deviations of 4.89-11.4 % and showed good linearity in the range of 0.02-10 μg L and 0.01-1.00 mg L, respectively. The recoveries for 20 fentanyl analogues in the low (at μg L level) and high (at mg L level) concentration spiked samples were in the range of 77.7-114 % and 83.9-116 %, which demonstrated the application potential of the proposed method for the determination of fentanyl analogues with low and high concentration in real case samples. In addition, the matrix effect and the cross-reactivity were also proved to not interfere with quantitation of targeted fentanyl analogues. Thus, the developed method showed high sensitivity and good accuracy, which makes it suitable for the rapid detection of fentanyl analogues for customs and border service as well as pharmaceuticals.
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http://dx.doi.org/10.1016/j.jpba.2021.114100DOI Listing
July 2021

In silico network pharmacology and in vivo analysis of berberine-related mechanisms against type 2 diabetes mellitus and its complications.

J Ethnopharmacol 2021 Aug 3;276:114180. Epub 2021 May 3.

Endocrinology Department, Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China. Electronic address:

Ethnopharmacological Relevance: Berberine (BBR), extracted from the traditional medicinal plant Coptis chinensis Franch., has been widely used for the treatment of type 2 diabetes mellitus (T2DM) and its complications.

Aim Of The Study: To determine the potential pharmacological mechanisms underlying BBR therapeutic effect on T2DM and its complications by in silico network pharmacology and experimental in vivo validation.

Materials And Methods: A predictive network depicting the relationship between BBR and T2DM was designed based on information collected from several databases, namely STITCH, CHEMBL, PharmMapper, TTD, Drugbank, and PharmGKB. Identified overlapping targets related to both BBR and T2DM were crossed with information on biological processes (BPs) and molecular/signaling pathways using the DAVID platform and Cytoscape software. Three candidate targets identified with the BBR-T2DM network (RXRA, KCNQ1 and NR3C1) were evaluated in the C57BL/6J mouse model of T2DM. The mice were treated with BBR or metformin for 10 weeks. Weight, fasting blood glucose (FBG), oral glucose tolerance, and expression levels of the three targets were evaluated.

Results: A total of 31 targets of BBR that were also related to T2DM were identified, of which 14 had already been reported in previous studies. Furthermore, these 31 overlapping targets were enriched in 21 related BPs and 18 pathways involved in T2DM treatment. The identified BP-target-pathway network revealed the underlying mechanisms of BBR antidiabetic activity were mediated by core targets such as RXRA, KCNQ1, and NR3C1. In vivo experiments further confirmed that treatment with BBR significantly reduced weight and FBG and alleviated insulin resistance in T2DM mice. Moreover, BBR treatment promoted RXRA expression, whereas it reduced KCNQ1 and NR3C1 expression in the liver.

Conclusion: Using network pharmacology and a T2DM mouse model, this study revealed that BBR can effectively prevent T2DM symptoms through vital targets and multiple signaling pathways. Network pharmacology provides an efficient, time-saving approach for therapeutic research and the development of new drugs.
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http://dx.doi.org/10.1016/j.jep.2021.114180DOI Listing
August 2021

Transforming growth factor‑β1 functions as a competitive endogenous RNA that ameliorates intracranial hemorrhage injury by sponging microRNA‑93‑5p.

Mol Med Rep 2021 Jul 6;24(1). Epub 2021 May 6.

Department of Neurology, The Affiliated Hospital of Jiujiang University, Jiujiang, Jiangxi 332000, P.R. China.

Intracerebral hemorrhage (ICH) has the highest mortality rate of all stroke subtypes but an effective treatment has yet to be clinically implemented. Transforming growth factor‑β1 (TGF‑β1) has been reported to modulate microglia‑mediated neuroinflammation after ICH and promote functional recovery; however, the underlying mechanisms remain unclear. Non‑coding RNAs such as microRNAs (miRNAs) and competitive endogenous RNAs (ceRNAs) have surfaced as critical regulators in human disease. A known miR‑93 target, nuclear factor erythroid 2‑related factor 2 (Nrf2), has been shown to be neuroprotective after ICH. It was hypothesized that TGF‑β1 functions as a ceRNA that sponges miR‑93‑5p and thereby ameliorates ICH injury in the brain. Short interfering RNA (siRNA) was used to knock down TGF‑β1 and miR‑93 expression was also pharmacologically manipulated to elucidate the mechanistic association between miR‑93‑5p, Nrf2, and TGF‑β1 in an model of ICH (thrombin‑treated human microglial HMO6 cells). Bioinformatics predictive analyses showed that miR‑93‑5p could bind to both TGF‑β1 and Nrf2. It was found that neuronal miR‑93‑5p was dramatically decreased in these HMO6 cells, and similar changes were observed in fresh brain tissue from patients with ICH. Most importantly, luciferase reporter assays were used to demonstrate that miR‑93‑5p directly targeted Nrf2 to inhibit its expression and the addition of the TGF‑β1 untranslated region restored the levels of Nrf2. Moreover, an miR‑93‑5p inhibitor increased the expression of TGF‑β1 and Nrf2 and decreased apoptosis. Collectively, these results identified a novel function of TGF‑β1 as a ceRNA that sponges miR‑93‑5p to increase the expression of neuroprotective Nrf2 and decrease cell death after ICH. The present findings provided evidence to support miR‑93‑5p as a potential therapeutic target for the treatment of ICH.
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http://dx.doi.org/10.3892/mmr.2021.12138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127068PMC
July 2021

Perfusion image guided mechanical thrombectomy combined with tirofiban successfully revascularize systemic lupus erythematosus related acute large vessel occlusion: A case report.

Medicine (Baltimore) 2021 May;100(18):e25779

Department of Neurological Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.

Rationale: Systemic lupus erythematosus (SLE) is an important cause of stroke, more than a half the cases present as acute ischemic stroke. Thrombolysis is an effective choice in most cases, but for large vessel occlusion, mechanical thrombectomy is more effective. Here we reported a case of SLE-related stroke with left middle cerebral artery (MCA) occlusion, who was successfully treated by MT and tirofiban.

Patient Concern: A 38-year-old female suffered from right hemiplegia and aphasia for 8 hours. She was diagnosed with SLE 20 years ago, and neuropsychiatric SLE was considered 8 months before this onset. One month ago, glucocorticoids were discontinued by herself because of deterioration of bilateral femoral head osteonecrosis.

Diagnosis: Left MCA occlusion was confirmed by computed tomography perfusion.

Intervention: Immediate mechanical thrombectomy was performed and tirofiban was given to prevent re-occlusion of left MCA. Twenty fourhours later oral antiplatelet was given after intracranial hemorrhage was ruled out.

Outcomes: Her neurological symptom improved several days later, and she was transferred to further rehabilitation. At 4 months follow-up she can live independently with mild hypophrasia. There was no further events of ischemic stroke in 1-year follow-up.

Lessons: Mechanical thrombectomy is a highly effective and indispensable treatment for SLE related large vessel occlusion. In addition, tirofiban may reduce vessel reocclusion in special cases such as SLE and artery stenosis.
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http://dx.doi.org/10.1097/MD.0000000000025779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104300PMC
May 2021

Genomic analysis of host gene responses to cerebral Plasmodium falciparum malaria.

Immun Inflamm Dis 2021 May 4. Epub 2021 May 4.

Department of Pathology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Introduction: A vaccine for malaria is urgently required but no vaccine has yet shown satisfactory protective efficacy especially for Plasmodium falciparum. P. falciparum infection can progress to cerebral malaria (CM), a neurological syndrome with exceedingly high mortality. Designing effective P. falciparum vaccines require more understanding of the protective immune response while the host immune response to CM and the mechanisms are still elusive. Here, we aim to identify host gene responses to CM and host gene networks associated with CM pathogenesis.

Methods: An innovative genomic analysis strategy, the weighted gene coexpression network analysis (WGCNA) combined with differential gene expression analysis, was used in this study. Data for analysis contain 93 whole blood samples, derived from two previous public transcriptome datasets.

Results: This approach led to the identification of numerous differentially expressed human transcripts and dozens of coexpression gene modules. We further identified nine key genes, including MBP, SAMSN1, PSMF1, SLC39A8, EIF3B, SMPDL3A, FABP5, SPSB3, and SHARPIN, of which the last four genes were first identified to be related to CM in the present study.

Conclusion: The results provided a comprehensive characterization of host gene expression profiles in CM and offered some new insight into malaria vaccine design. These identified key genes could be potential targets or immune modulators for novel therapeutic interventions of CM.
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http://dx.doi.org/10.1002/iid3.436DOI Listing
May 2021

Elimination of human cytomegalovirus DNA degradation in urine.

J Med Virol 2021 May 3. Epub 2021 May 3.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Centers of Biological Products, School of Life Science and School of Public Health, Xiamen University, Xiamen, Fujian, China.

Congenital cytomegalovirus infection (cCMVi) is an important cause of sensorineural hearing loss in newborns. Detection of human cytomegalovirus (HCMV) DNA in urine has been used to screen for cCMVi in newborns. However, the matrix effect of urine on HCMV DNA detection is unclear. To evaluate the matrix effect of urine on HCMV DNA detection and optimize the sample process strategy to eliminate or minimize the impact of urine on HCMV DNA detection, DNA in spiked samples was extracted using different DNA extraction methods, and urine samples that could inhibit HCMV DNA detection were mixed to evaluate the inhibitory substances, inhibitory mechanism, and elimination of the inhibitory effect. The optimal urine sample process strategy was evaluated using 42 adult female urine samples and 42 newborn urine samples spiked with HCMV. Some urine samples were found to inhibit HCMV DNA detection due to DNA degradation. The addition of ≥5 mM EDTA to the urine before extraction eliminated the inhibitory effect of urine and did not affect the detection results of urine exhibiting no inhibition. Of the 42 adult female and 42 newborn urine samples, four and two samples, respectively, could inhibit HCMV DNA detection. However, the inhibitory effects of these six urine samples were eliminated after the addition of EDTA. The collective results indicate that the addition of EDTA can completely eliminate the impact of inhibitors present in urine on HCMV DNA extraction and improve the detection of HCMV in urine.
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http://dx.doi.org/10.1002/jmv.27035DOI Listing
May 2021

Serum Neurofilament Dynamics Predicts Cognitive Progression in de novo Parkinson's Disease.

J Parkinsons Dis 2021 Apr 30. Epub 2021 Apr 30.

Department of Neurology and Institute of Neurology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Background: Neurofilament light (NfL) can reflect the extent of neuron/axon damage, thus providing an opportunity to examine the severity and progression of the diseases with such damage.

Objective: Whether serum NfL can be used as an indicator to monitor the cognitive progress of de novo Parkinson's disease (PD) remains unclear.

Methods: In this research, 144 healthy controls and 301 de novo PD patients from Parkinson's Progression Markers Initiative (PPMI) were recruited. Linear mixed effects models were used to examine the associations of baseline/longitudinal serum NfL with cognitive decline. Cox regression was used to detect cognitive progression in PD participants.

Results: We found PD patients had higher serum NfL than controls at baseline (p = 0.031), and NfL increase was faster in PD group (p < 0.001). Both baseline serum NfL and its rate of change predicted measurable cognitive decline in early PD (MoCA, β= -0.014, p < 0.001; β= -0.002, p < 0.001, respectively). Additionally, we observed that NfL levels were also able to predict progression in different diagnostic groups and Amyloid- PD and Amyloid+PD groups. After an average follow-up of 6.37±1.84 years, the baseline NfL of the third tertile of high concentrations was associated with a future high risk of PD dementia (adjusted HR 6.33, 95% CI 2.62-15.29, p < 0.001).

Conclusion: In conclusion, our results indicated that the serum NfL concentration could function as an easily accessible biomarker to monitor the severity and progression of cognitive decline in PD.
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http://dx.doi.org/10.3233/JPD-212535DOI Listing
April 2021

NiCoS microflowers as peroxidase mimic: A multi-functional platform for colorimetric detection of glucose and evaluation of antioxidant behavior.

Talanta 2021 Aug 23;230:122337. Epub 2021 Mar 23.

Beijing Key Laboratory of Environmentally Harmful Chemical Analysis, College of Chemistry, Beijing University of Chemical Technology, Beijing, 100029, PR China. Electronic address:

The regular design of atomic composition of materials endows a diverse range of enzyme mimics types and increases a broader application prospect. In this study, we designed a nickel-cobalt mixed metal sulfide and demonstrated that the as-prepared NiCoS microflowers possessed intrinsic peroxidase-like activity. Nickel-cobalt sulfide (NiCoS) possessed high electron transfer capacity, which lead to good peroxidase-like activity. Compared with the reported enzyme-like materials, NiCoS exhibited a smaller Km and a stronger affinity with substrate. A colorimetric assay was developed for the direct detection of hydrogen peroxide and indirect detection of glucose over a wide linear range (HO was 20-200 μM, and glucose was 20-1000 μM) with a low detection limit (HO was 5.19 μM, and glucose was 8.24 μM). Furthermore, a NiCoS based platform was established to study the antioxidant behavior of three antioxidants. The antioxidant capacities of the antioxidants were found to rank in the order: tannic acid (TA) > ascorbic acid (AA)> gallic acid (GA). Moreover, the three antioxidants showed different inhibition mechanisms. This study indicated a new and important application field for NiCoS and provides a basis for the rational design of enzyme-like mimics in future research.
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http://dx.doi.org/10.1016/j.talanta.2021.122337DOI Listing
August 2021

Surveillance of adverse events following immunisation in Australia annual report, 2019.

Commun Dis Intell (2018) 2021 Apr 30;45. Epub 2021 Apr 30.

National Centre for Immunisation Research and Surveillance, The University of Sydney and The Children's Hospital at Westmead, Sydney, Australia.

Abstract: This report summarises Australian spontaneous surveillance data for adverse events following immunisation (AEFI) for 2019 reported to the Therapeutic Goods Administration (TGA) and describes reporting trends over the 20-year period from 1 January 2000 to 31 December 2019. There were 3,782 AEFI records for vaccines administered in 2019, an annual AEFI reporting rate of 14.9 per 100,000 population. There was an 11.8% decrease in the overall AEFI reporting rate in 2019 compared to 2018 (16.9 per 100,000 population). This decrease in the AEFI reporting rate in 2019 was mainly attributable to a decline in reported adverse events related to the human papillomavirus (HPV), dTpa, meningococcal ACWY and seasonal influenza vaccines. AEFI reporting rates for most individual vaccines in 2019 were similar to 2018. The most commonly-reported adverse events were injection site reaction (35.8%), rash (16.6%), pyrexia (15.3%), vomiting (8.1%), urticaria (5.8%), pain (5.8%) and headache (5.7%). There were five deaths reported to the TGA. In one report, the timing and clinical findings were consistent with a causal association with vaccination. In the remaining four reports, no clear causal relationship with vaccination was found.
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http://dx.doi.org/10.33321/cdi.2021.45.23DOI Listing
April 2021

Computational Analysis Predicts Hundreds of Coding lncRNAs in Zebrafish.

Biology (Basel) 2021 Apr 26;10(5). Epub 2021 Apr 26.

Center for Circadian Clocks, Soochow University, Suzhou 215123, China.

Recent studies have demonstrated that numerous long noncoding RNAs (ncRNAs having more than 200 nucleotide base pairs (lncRNAs)) actually encode functional micropeptides, which likely represents the next regulatory biology frontier. Thus, identification of coding lncRNAs from ever-increasing lncRNA databases would be a bioinformatic challenge. Here we employed the Coding Potential Alignment Tool (CPAT), Coding Potential Calculator 2 (CPC2), LGC web server, Coding-Non-Coding Identifying Tool (CNIT), RNAsamba, and MicroPeptide identification tool (MiPepid) to analyze approximately 21,000 zebrafish lncRNAs and computationally to identify 2730-6676 zebrafish lncRNAs with high coding potentials, including 313 coding lncRNAs predicted by all the six bioinformatic tools. We also compared the sensitivity and specificity of these six bioinformatic tools for identifying lncRNAs with coding potentials and summarized their strengths and weaknesses. These predicted zebrafish coding lncRNAs set the stage for further experimental studies.
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http://dx.doi.org/10.3390/biology10050371DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145020PMC
April 2021

Simulation and Optimization of SNAP-Taper Coupling System in Displacement Sensing.

Sensors (Basel) 2021 Apr 22;21(9). Epub 2021 Apr 22.

State Key Laboratory of Precision Electronic Manufacturing Technology and Equipment, Guangdong Provincial Key Laboratory of Micro-Nano Manufacturing Technology and Equipment, Mechanical and Electrical Engineering, Guangdong University of Technology, Guangzhou 510006, China.

Sensing applications based on whispering gallery mode (WGM) microcavities have attracted extensive attention recently, especially in displacement sensing applications. However, the traditional displacement sensing scheme based on shift in a single resonance wavelength, has a lot of drawbacks. Herein, a novel displacement sensing scheme based on the surface nanoscale axial photonics (SNAP) is proposed to achieve a wide range and high-resolution displacement sensor through analyzing the transmittance of multiple axial modes. By analyzing the surface plot of the resonance spectrum with different coupling positions, the ideal coupling parameters and ERV for displacement sensing are obtained. In the following, displacement sensing with high sensitivity and a wide range is theoretically realized through adjusting the sensitivity threshold and the number of modes. Finally, we present our views on the current challenges and the future development of the displacement sensing based on an SNAP resonator. We believe that a comprehensive understanding on this sensing scheme would significantly contribute to the advancement of the SNAP resonator for a broad range of applications.
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http://dx.doi.org/10.3390/s21092947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122849PMC
April 2021