Publications by authors named "Han Huang"

224 Publications

Goose nephritic astrovirus infection increases autophagy, destroys intercellular junctions in renal tubular epithelial cells, and damages podocytes in the kidneys of infected goslings.

Vet Microbiol 2021 Oct 8;263:109244. Epub 2021 Oct 8.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.

Goose nephritic astrovirus (GNAstV) has recently been identified, which causes kidney swelling and visceral gout in goslings. However, the pathological changes in kidney tissue due to GNAstV infection have not yet been described. In the study, fifty goslings were orally infected with GNAstV, and fifty goslings received PBS as a control. Kidney tissue was collected at different days following infection (dpi) to assess the injury. GNAstV infection reduced body weight, increased the relative weight of the kidney, and increased serum uric acid and creatinine levels. GNAstV was found within renal epithelial cells, and the viral load in the kidney peaked at 7 dpi. Pale and swollen kidney tissue was observed in infected goslings, especially at 5 and 7 dpi. GNAstV infection caused degeneration and necrosis of renal epithelial cells, structural destruction of the brush border, glycogen deposition in the glomerular mesangium, increased fibrosis, and infiltration of inflammatory cells into the renal interstitium. Moreover, swollen mitochondria, broken mitochondrial ridges, autophagosomes, and autophagolysosomes were observed under ultrahistopathological examination. GNAstV infection increased levels of LC3B, ATG5, and Beclin 1, and decreased p62, and downregulated WT1 mRNA and upregulated desmin mRNA. At early stages, GNAstV infection decreased expression of intercellular junction-related genes, including ZO-1, occludin, claudin-10, and catenin-α2. In conclusion, GNAstV infection causes renal epithelial cell autophagy, destruction of brush border and intercellular junctions, podocyte damage, and increased fibrosis, ultimately resulting in damage to the kidney.
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http://dx.doi.org/10.1016/j.vetmic.2021.109244DOI Listing
October 2021

Micromechanics of Machining and Wear in Hard and Brittle Materials.

J Am Ceram Soc 2021 Jan 27;104(1):5-22. Epub 2020 Sep 27.

Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Hard and brittle solids with covalent/ionic bonding are used in a wide range of modern-day manufacturing technologies. Optimization of a shaping process can shorten manufacturing time and cost of component production, and at the same time extend component longevity. The same process may contribute to wear and fatigue degradation in service. Educated development of advanced finishing protocols for this class of solids requires a comprehensive understanding of damage mechanisms at small-scale contacts from a materials science perspective. In this article the fundamentals of brittle-ductile transitions in indentation stress fields are surveyed, with distinctions between axial and sliding loading and blunt and sharp contacts. Attendant deformation and removal mechanisms in microcontact processes are analyzed and discussed in the context of brittle and ductile machining and severe and mild wear. The central role of material microstructure in material removal modes is demonstrated.
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http://dx.doi.org/10.1111/jace.17502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460072PMC
January 2021

The osteoporosis risk variant rs9820407 at 3p22.1 acts as an allele-specific enhancer to regulate CTNNB1 expression by long-range chromatin loop formation.

Bone 2021 Dec 28;153:116165. Epub 2021 Aug 28.

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China. Electronic address:

Previous powerful genome-wide association studies (GWASs) and whole-genome sequencing have identified multiple single-nucleotide polymorphisms (SNPs) located over 69 kb upstream of CTNNB1 at 3p22.1 locus associated with osteoporosis. The CTNNB1 gene encodes β-catenin that is an integral part of adherens junctions and the primary mediator of the canonical Wnt signaling pathway. The causal variants and underlying molecular mechanisms of the osteoporosis susceptibility locus 3p22.1 remains unknown. Through comprehensive computational analyses, including expression quantitative trait locus (eQTL), high-throughput chromatin interaction (Hi-C), epigenomic and functional annotation, four enhancer SNPs (rs9820407, rs9878224, rs454690 and rs9832204) were prioritized as potential causal SNPs at 3p22.1 for osteoporosis. Rs9820407 displayed the strongest enhancer activity in dual-luciferase assays. Specifically, the minor rs9820407-A can preferentially bind transcription factor FOXC1, elevate the enhancer activity and increase CTNNB1 expression. The architectural protein CTCF was presumably involved in long-range chromatin interaction between rs9820407 and CTNNB1. Our study provided a mechanistic insight into how noncoding enhancer SNP rs9820407 distally regulates CTNNB1 expression and modulates osteoporosis risk.
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http://dx.doi.org/10.1016/j.bone.2021.116165DOI Listing
December 2021

Discovery of 4-arylthiophene-3-carboxylic acid as inhibitor of ANO1 and its effect as analgesic agent.

Acta Pharm Sin B 2021 Jul 17;11(7):1947-1964. Epub 2020 Nov 17.

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.

Anoctamin 1 (ANO1) is a kind of calcium-activated chloride channel involved in nerve depolarization. ANO1 inhibitors display significant analgesic activity by the local peripheral and intrathecal administration. In this study, several thiophenecarboxylic acid and benzoic acid derivatives were identified as novel ANO1 inhibitors through the shape-based virtual screening, among which the 4-arylthiophene-3-carboxylic acid analogues with the best ANO1 inhibitory activity were designed, synthesized and compound (IC = 0.79 μmol/L) was finally obtained. Compound selectively inhibited ANO1 without affecting ANO2 and intracellular Ca concentration. Subsequently, the analgesic effect was investigated by intragastric administration in pain models. Compound significantly attenuated allodynia which was induced by formalin and chronic constriction injury. Through homology modeling and molecular dynamics, the binding site was predicted to be located near the calcium-binding region between 6 and 8. Our study validates ANO1 inhibitors having a significant analgesic effect by intragastric administration and also provides selective molecular tools for ANO1-related research.
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http://dx.doi.org/10.1016/j.apsb.2020.11.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343189PMC
July 2021

A new flexible pressure sensor contributes to the early diagnosis of acute compartment syndrome.

Int J Clin Exp Pathol 2021 15;14(6):768-775. Epub 2021 Jun 15.

School of Electronic and Optical Engineering, Nanjing University of Science and Technology Nanjing, China.

Acute compartment syndrome (ACS) is a surgical emergency, caused by the sharp increase of interstitial pressure within a closed osteofascial compartment, which can impair local circulation and functions. A non-invasive sensor is needed of satisfactory sensitivity to continuously monitor the alterations of the ACS pressure, which could be used as a supplementary means in the early diagnosis of ACS. A prepared "pasting-type" flexible pressure sensor was used to establish an extracorporeal pig-skin model with a soft-tissue expander device to simulate compartment syndrome conditions. An acrylic panel was inserted into the pig skin, allowing the soft-tissue expander to expand in one direction, which is similar to the movements of a patient's bones in real life. The touch spot of the flexible pressure sensor was attached to the rind by medical tape, to record the internal and external pressure data. Relationships between the internal and external pressures at different thickness (0.87 mm, 3.53 mm and 3.97 mm), as well as that of the 3.97 mm thickness under various initial internal pressures (0.5 mmHg, 25 mmHg and 44 mmHg) were measured. Significant differences were observed in the range of internal pressures at various pig-skin thickness. After adding the acrylic panel, the measured ranges were significantly increased, with the lowest measurable internal pressure being 5 mmHg. Moreover, alterations in external pressure were also greater than in models without acrylic panels. The external pressure measured by the sensor was able to reflect an increase in intra-organizational pressure. This may be a new non-invasive and sustainable method for early diagnosis of ACS.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255205PMC
June 2021

Balancing Constraints and Objectives by Considering Problem Types in Constrained Multiobjective Optimization.

IEEE Trans Cybern 2021 Jul 8;PP. Epub 2021 Jul 8.

Constrained multiobjective optimization problems widely exist in real-world applications. To handle them, the balance between constraints and objectives is crucial, but remains challenging due to non-negligible impacts of problem types. In our context, the problem types refer particularly to those determined by the relationship between the constrained Pareto-optimal front (PF) and the unconstrained PF. Unfortunately, there has been little awareness on how to achieve this balance when faced with different types of problems. In this article, we propose a new constraint handling technique (CHT) by taking into account potential problem types. Specifically, inspired by the prior work, problems are classified into three primary types: 1) I; 2) II; and 3) III, with the constrained PF being made up of the entire, part and none of the unconstrained counterpart, respectively. Clearly, any problem must be one of the three types. For each possible type, there exists a tailored mechanism being used to handle the relationships between constraints and objectives (i.e., constraint priority, objective priority, or the switch between them). It is worth mentioning that exact problem types are not required because we just consider their possibilities in the new CHT. Conceptually, we show that the new CHT can make a tradeoff among different types of problems. This argument is confirmed by experimental studies performed on 38 benchmark problems, whose types are known, and a real-world problem (with unknown types) in search-based software engineering. Results demonstrate that within both decomposition-based and nondecomposition-based frameworks, the new CHT can indeed achieve a good tradeoff among different problem types, being better than several state-of-the-art CHTs.
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http://dx.doi.org/10.1109/TCYB.2021.3089633DOI Listing
July 2021

Goose Nephritic Astrovirus Infection of Goslings Induces Lymphocyte Apoptosis, Reticular Fiber Destruction, and CD8 T-Cell Depletion in Spleen Tissue.

Viruses 2021 06 9;13(6). Epub 2021 Jun 9.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

The emergence of a novel goose nephritic astrovirus (GNAstV) has caused economic losses to the Chinese goose industry. High viral load is found in the spleen of goslings infected with GNAstV, but pathological injuries to the spleen due to GNAstV are largely unknown. In this study, 50 two-day-old goslings were infected orally with GNAstV, and 50 goslings were treated with PBS as control. Spleens were collected at different times following infection to assess damage. GNAstV infection caused visceral gout and urate deposition in joints, and resulted in 16% mortality. GNAstV was found in the lymphocytes and macrophages within the spleen. Lymphocyte loss, especially around the white pulp, and destruction and decline in the number of reticular fibers was observed in GNAstV-infected goslings. Moreover, in GNAstV-infected goslings, ultrahistopathological examination found that splenic lymphocytes exhibited condensed chromatin and apoptotic bodies, and reticular cells displayed damage to plasma membrane integrity and swollen mitochondria. Furthermore, TUNEL staining confirmed apoptosis of lymphocytes, and the mRNA levels of Fas and FasL were significantly increased in the GNAstV-infected goslings. In addition, GNAstV infection reduced the number and protein expression of CD8. In conclusion, GNAstV infection causes lymphocyte depletion, reticular cell necrosis, reticular fiber destruction, lymphocyte apoptosis, and reduction in CD8 levels, which contribute to spleen injury.
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http://dx.doi.org/10.3390/v13061108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229047PMC
June 2021

Creating a Dual-Functional 2D Perovskite Layer at the Interface to Enhance the Performance of Flexible Perovskite Solar Cells.

Small 2021 Aug 26;17(32):e2102368. Epub 2021 Jun 26.

Hunan Key Laboratory for Super-microstructure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha, 410083, China.

Flexible perovskite solar cells (f-PSCs) have been attracting tremendous attention due to their potentially commercial prospects in flexible energy system and mobile energy system. Reducing the energy barriers and charge extraction losses at the interfaces between perovskite and charge transport layers is essential to improve both efficiency and stability of f-PSCs. Herein, 4-trifluoromethylphenylethylamine iodide (CF PEAI) is introduced to form a 2D perovskite at the interface between perovskite and hole transport layer (HTL). It is found that the 2D perovskite plays a dual-functional role in aligning energy band between perovskite and HTL and passivating the traps in the 3D perovskite, thus reducing energy loss and charge carrier recombination at the interface, facilitating the hole transfer from perovskite to the Spiro-OMeTAD. Consequently, the photovoltaic performance of f-PSCs is significantly improved, leading to a power conversion efficiency (PCE) of 21.1% and a certified PCE of 20.5%. Furthermore, the long-term stability of f-PSCs is greatly improved through the protection of 2D perovskite layer to the underlying 3D perovskite. This work provides an excellent strategy to produce efficient and stable f-PSCs, which will accelerate their potential applications.
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http://dx.doi.org/10.1002/smll.202102368DOI Listing
August 2021

Emerging photothermal-derived multimodal synergistic therapy in combating bacterial infections.

Chem Soc Rev 2021 Aug;50(15):8762-8789

Frontiers Science Center for Flexible Electronics (FSCFE), Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials & Engineering (IBME), Northwestern Polytechnical University, 127 West Youyi Road, Xi'an 710072, China. and State Key Laboratory of Organic Electronics and Information Displays (SKLOEID), Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China and Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), Nanjing 211816, China.

Due to the emerging bacterial resistance and the protection of tenacious biofilms, it is hard for the single antibacterial modality to achieve satisfactory therapeutic effects nowadays. In recent years, photothermal therapy (PTT)-derived multimodal synergistic treatments have received wide attention and exhibited cooperatively enhanced bactericidal activity. PTT features spatiotemporally controllable generation of hyperthermia that could eradicate bacteria without inducing resistance. The synergy of it with other treatments, such as chemotherapy, photo-dynamic/catalytic therapy (PDT/PCT), immunotherapy, and sonodynamic therapy (SDT), could lower the introduced laser density in PTT and avoid undesired overheating injury of normal tissues. Simultaneously, by heat-induced improvement of the bacterial membrane permeability, PTT is conducive for accelerated intracellular permeation of chemotherapeutic drugs as well as reactive oxygen species (ROS) generated by photosensitizers/sonosensitizers, and could promote infiltration of immune cells. Thereby, it could solve the currently existing sterilization deficiencies of other combined therapeutic modes, for example, bacterial resistance for chemotherapy, low drug permeability for PDT/PCT/SDT, adverse immunoreactions for immunotherapy, etc. Admittedly, PTT-derived synergistic treatments are becoming essential in fighting bacterial infection, especially those caused by antibiotic-resistant strains. This review firstly presents the classical and newly reported photothermal agents (PTAs) in brief. Profoundly, through the introduction of delicately designed nanocomposite platforms, we systematically discuss the versatile photothermal-derived multimodal synergistic therapy with the purpose of sterilization application. At the end, challenges to PTT-derived combinational therapy are presented and promising synergistic bactericidal prospects are anticipated.
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http://dx.doi.org/10.1039/d1cs00074hDOI Listing
August 2021

Thermodynamic properties and hysteresis loops in a hexagonal core-shell nanoparticle.

J Mol Graph Model 2021 09 15;107:107967. Epub 2021 Jun 15.

School of Environmental and Chemical Engineering, Shenyang University of Technology, Shenyang, 110870, China; School of Sciences, Shenyang University of Technology, Shenyang, 110870, China.

We applied Monte Carlo simulation to investigate the thermodynamic properties and hysteresis loops of the hexagonal core-shell nanoparticle described by a ferrimagnetic mixed-spin (3/2, 5/2) Ising model. The results revealed the significance of the single-ion anisotropy, exchange coupling, external magnetic field in dominating various thermodynamic quantities and hysteresis loops. We obtained the variation of the critical temperature with various parameters. Under certain parameter conditions, the system may exhibit rich multiple-loop hysteresis behaviors, depending on the competition among the physical parameters.
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http://dx.doi.org/10.1016/j.jmgm.2021.107967DOI Listing
September 2021

Application of microRNA in Human Osteoporosis and Fragility Fracture: A Systemic Review of Literatures.

Int J Mol Sci 2021 May 15;22(10). Epub 2021 May 15.

Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, Taiwan.

MicroRNAs (miRNAs) could serve as ideal entry points to the deregulated pathways in osteoporosis due to their relatively simple upstream and downstream relationships with other molecules in the signaling cascades. Our study aimed to give a comprehensive review of the already identified miRNAs in osteoporosis from human blood samples and provide useful information for their clinical application. A systematic literature search for relevant studies was conducted in the Pubmed database from inception to December 2020. We set two essential inclusion criteria: human blood sampling and design of controlled studies. We sorted the results of analysis on human blood samples according to the study settings and compiled the most promising miRNAs with analyzed diagnostic values. Furthermore, in vitro and in vivo evidence for the mechanisms of the identified miRNAs was also illustrated. Based on both diagnostic value and evidence of mechanism from in vitro and in vivo experiments, miR-23b-3p, miR-140-3p, miR-300, miR-155-5p, miR-208a-3p, and miR-637 were preferred candidates in diagnostic panels and as therapeutic agents. Further studies are needed to build sound foundations for the clinical usage of miRNAs in osteoporosis.
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http://dx.doi.org/10.3390/ijms22105232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156577PMC
May 2021

Quantitative determining of pre-operative osteotomy plan for severe spinal deformity: an analysis of 131 consecutive Yang's A type cases from single center.

Eur Spine J 2021 May 18. Epub 2021 May 18.

Spine Surgery Center, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, China.

Purpose: This study aimed to quantify osteotomy protocol for severe spinal deformity correction based on pre-operative demographic, clinical and radiologic parameters.

Methods: A total of 131 Yang's A type severe spinal deformity patients were included. All patients received one of following osteotomies: Ponte/2 (Ponte osteotomy/Grade 2 osteotomy, n = 30), PSO/3 (pedicle subtraction osteotomy/Grade 3 osteotomy, n = 19), BDBO/4 (bone-disc-bone osteotomy/Grade 4 osteotomy, n = 26), SVCR/5 (single-level vertebral column resection/Grade 5 osteotomy, n = 38) and MVCR/6 (multilevel vertebral column resection/Grade 6 osteotomy, n = 18). Demographic, clinical and radiologic characteristics were compared among groups.

Results: Tukey's test identified 6 significant variables between paired groups: age for MVCR/6 versus Ponte/2 + PSO/3 + BDBO/4 + SVCR/5 groups; bending Cobb angle for Ponte/2 versus MVCR/6; sagittal deformity angular ratio (S-DAR) for Ponte/2 + PSO/3 versus BDBO/3 + SVCR/5 versus MVCR/6; pre-operative scoliosis for Ponte/2 versus SVCR/5; total-DAR (T-DAR) and pre-operative kyphosis for Ponte/2 + PSO/3 versus BDBO/4 + SVCR/5 + MVCR/6. Receiver Operating Characteristic (ROC) analysis showed the Area under the Curve (AUC) for the 6 variables ranged from 0.69 to 0.839. Multivariate k-means clustering analysis showed that Ponte/2 + PSO/3, BDBO/4 + SVCR/5, and MVCR/6 were three comparatively significant clusters, which could be discriminated by pre-operative kyphosis, T-DAR, age and S-DAR.

Conclusion: This study showed that osteotomy plan of severe spinal deformity could be determined as follows: Firstly, Ponte/2 + PSO/3 and BDBO/4 + SVCR/5 + MVCR/6 groups can be divided by either T-DAR (cutoff = 28) or the Cobb angle of pre-operative maximum kyphosis (cutoff = 100). Secondly, Ponte/2 + PSO/3 group could be further dichotomized into Ponte/2 and PSO/3 by age (cutoff = 18). Finally, BDBO/4 + SVCR/5 + MVCR/6 group could be divided into BDBO/4 + SVCR/5 and MVCR/6 groups by S-DAR (cutoff = 20).
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http://dx.doi.org/10.1007/s00586-021-06840-1DOI Listing
May 2021

Effect of continuous infusion of a subhypnotic dose of propofol on nausea and vomiting after carboprost administration at cesarean delivery: A randomized, double-blind, placebo-controlled trial.

Int J Gynaecol Obstet 2021 May 16. Epub 2021 May 16.

Department of Anesthesiology and Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.

Objective: To investigate whether continuous infusion of propofol at a subhypnotic dose prevents nausea and vomiting following carboprost administration at cesarean delivery.

Methods: A prospective, randomized, double-blind, placebo-controlled trial conducted at West China Second University Hospital, from June 28, 2017 to January 30, 2018. Pregnant women were randomly allocated to propofol or saline infusion immediately before receiving carboprost at cesarean delivery under combined spinal-epidural (CSE) anesthesia. Propofol was given at an infusion rate of 1.0 mg/kg/h following a loading dose of 0.3 mg/kg. Primary outcome was incidence of intraoperative nausea and vomiting (IONV). Potential sedative effect of propofol infusion was assessed using Modified Observer's Assessment of Alertness and Sedation (MOAA/S) scoring and continuous Bispectral Index (BIS) monitoring.

Results: The incidence of IONV was lower in patients who received propofol compared with saline (46.7% vs 76.7%, OR 0.27; 95% CI, 0.092-0.78, P = 0.016 for nausea; 26.7% vs 53.3%, OR 0.50; 95% CI, 0.25-0.95, P = 0.032 for retching; 10.0% vs 50.0%, OR 0.11; 95% CI, 0.03-0.44, P < 0.001 for vomiting). There were no differences in MOAA/S scoring or BIS between the two groups.

Conclusion: A subhypnotic dose of propofol reduces the incidence of nausea and vomiting following carboprost administration at cesarean delivery under CSE anesthesia, without measurable effect on patients' consciousness or alertness. ClincalTrials.gov: NCT03185156.
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http://dx.doi.org/10.1002/ijgo.13742DOI Listing
May 2021

Effectiveness and safety of acupoint application for chronic obstructive pulmonary disease: A protocol for updated systematic review and meta-analysis.

Medicine (Baltimore) 2021 May;100(18):e25802

Affiliated Hospital of Liaoning University of Traditional Chinese Medicine.

Background: Acute exacerbation is a primary cause of repeated hospitalization and death in chronic obstructive pulmonary disease (COPD) patients. Therefore, how to control the symptoms of COPD at stable stage and reduce the number of acute exacerbation is a hot spot of medical research. Acupoint application (AA) is a significant part of external treatment of traditional Chinese medicine (TCM), Previous researches have reported that AA can be applied to the treatment of COPD. Nevertheless, its effectiveness is still inconclusive. This systematic review (SR) and meta-analysis is designed to appraise its effectiveness and safety for the treatment of patients with COPD.

Methods: Eight databases will be systematically retrieved from their inceptions to February 2021. Inclusion criteria are randomized control trials of AA combined with routine western medicine interventions in the treatment of COPD at stable stage. The primary outcomes we focus on comprise clinical effective rate, TCM symptom score, quality of life, dyspnea, exercise capacity, lung function, frequency of acute exacerbation, adverse events. The research screening, data extraction, and risk of bias assessment will be conducted by 2 individuals independently, and divergence will be adjudicated by a third senior investigator. The Stata 13.1 software will be used for meta-analysis. The confidence of evidence will be classified adopting grading of recommendations assessment, development and evaluation (GRADE) algorithm and methodological quality of this SR will be assessed using assessment of multiple systematic reviews-2 (AMSTAR-2) tool.

Results: This SR will provide evidence-based medical proof for the treatment of COPD at stable stage by AA combined with conventional western medicine interventions. The findings of this SR will be presented at relevant conferences and submitted for peer-review publication.

Conclusions: The findings of this SR will provide up-todated summary proof for evaluating the effectiveness and safety of AA for COPD.

Registration Number: INPLASY 202140080.
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http://dx.doi.org/10.1097/MD.0000000000025802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104243PMC
May 2021

[Effect of Long-time Postnatal Exposure to Sevoflurane on Causing Attention-deficit/Hyperactivity Disorder in Rats].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Mar;52(2):207-215

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, China.

Objective: To investigate whether long-term exposure to inhaled sevoflurane, a volatile anesthetic, causes abnormal activities and memory impairment related to attention-deficit/hyperactivity disorder (ADHD) in neonatal rats.

Methods: On postnatal day 5 (P5), Sprague-Dawley rats were randomly assigned to two sevoflurane subgroups and two control subgroups and underwent experimental intervention. The two sevoflurane (SEVO) subgroups were exposed to 3% sevoflurane for 2 h and 4 h respectively, while the two control subgroups were given pure oxygen for the same amount and duration. Behavioral tests, including open-field test (OFT), five-choice serial reaction time task (5-CSRTT), fear-conditioning (FC) and Morris water maze (MWM), were applied to evaluate changes in cognition, memory, anxiety and ADHD-related behavioral changes in the rats in adolescence (-P25) and in adulthood (-P65).

Results: In OFT, the SEVO 2 h and SEVO 4 h subgroups displayed activity level and exploratory behaviors similar to those of the control subgroups on P21 and P61, with no statistically significant difference identified in the data. 5-CSRTT results on P25 and P65 indicated no statistically significant difference between the SEVO subgroups and the control subgroups in regard to ADHD-related abnormal behaviors, including number of immature reaction, rate of correct response and omission rate. In the FC experiment, SEVO 4 h group had a shorter freezing period and longer period of freezing latency ( =0.029) in comparison to the control groups. The results of the MWM test showed that the escape latency period of rats in the SEVO 4 h group was significantly prolonged on the second day and the third day, compared to the control groups ( <0.05). The average swimming speed of SEVO groups did no exhibit any statistically significant difference on P69 or P76. The time the SEVO 4 h group spent in the target quadrant was significantly shorter than that of the control group ( =0.039) and percentage of distance traveled in the target quadrant was significantly reduced compared to that the control group ( =0.048).

Conclusion: The findings suggest that four hours of inhaled sevoflurane exposure in neonate rats may cause memory impairment, but does no increase risks for ADHD-related abnormal activities.
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http://dx.doi.org/10.12182/20210360601DOI Listing
March 2021

Disseminated Infection in a Non-HIV Infant With a Homozygous Private Variant of .

Front Cell Infect Microbiol 2021 15;11:605589. Epub 2021 Mar 15.

Department of Biochemistry, School of Basic Medical Science, Chengde Medical University, Chengde, China.

Objective: This study presents a relatively rare case of disseminated () infection in an HIV-negative patient.

Methods: An 8-month-old girl was hospitalized because of uncontrollable fever and cough for 6 days. Routine laboratory tests, biochemical detection, immunological tests, pathogenic examination, and imaging inspection were performed. Genetic tests of trio whole genome sequencing (Trio-WES), trio copy number sequencing (Trio-CNVseq), and Sanger sequencing were conducted to identify pathogenic variants. analysis of the sequence alignment and structural modeling results was carried out to study the possible pathogenicity of the identified variant. Western blotting was performed to investigate the expression of the identified gene at the protein level.

Results: Enhanced CT and MRI scanning demonstrated thymic dysplasia, diffuse pulmonary and liver nodules, and many balloon-like air sacs in both lungs. The white blood cell count, neutrophil count, and neutrophil ratio were normal or elevated. The patient was HIV-negative and bone marrow and blood culture showed infection. Total lymphocyte count, CD3+ T lymphocyte count, CD3+CD4+ T lymphocyte count, CD3+CD8+ T lymphocyte count, and NK cell count decreased, while the number of CD19 positive B cells increased. However, the ratio of CD3+CD4+:CD3+CD8+ T cells increased. Trio-WES identified a homozygous private variant of NM_006509: c.400_c.401insAGC/p.Lys134 delinsLysGln in and Sanger sequencing validated the result. Structural modeling indicated that the variant may be pathogenic. Reverse transcription-polymerase chain reaction and Western blot analysis showed that the expression of RelB in the patient was lower than that in the healthy controls at mRNA and protein levels.

Conclusion: This is the first report on disseminated infection in a patient with a homozygous private variant of .
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http://dx.doi.org/10.3389/fcimb.2021.605589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005656PMC
June 2021

Sodium leak channel contributes to neuronal sensitization in neuropathic pain.

Prog Neurobiol 2021 Jul 22;202:102041. Epub 2021 Mar 22.

Laboratory of Anesthesia & Critical Care Medicine, Translational Neuroscience Center, West China Hospital of Sichuan University, Chengdu 610041, Sichuan, China; Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu 610041, Sichuan, China. Electronic address:

Neuropathic pain affects up to 10 % of the total population and no specific target is ideal for therapeutic need. The sodium leak channel (NALCN), a non-selective cation channel, mediates the background Na leak conductance and controls neuronal excitability and rhythmic behaviors. Here, we show that increases of NALCN expression and function in dorsal root ganglion (DRG) and dorsal spinal cord contribute to chronic constriction injury (CCI)-induced neuropathic pain in rodents. NALCN current and neuronal excitability in acutely isolated DRG neurons and spinal cord slices of rats were increased after CCI which were decreased to normal levels by NALCN-siRNA. Accordingly, pain-related symptoms were significantly alleviated by NALCN-siRNA-mediated NALCN knockdown and completely prevented by NALCN-shRNA-mediated NALCN knockdown in rats or by conditional NALCN knockout in mice. Our results indicate that increases in NALCN expression and function contribute to CCI-induced neuronal sensitization; therefore, NALCN may be a novel molecular target for control of neuropathic pain.
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http://dx.doi.org/10.1016/j.pneurobio.2021.102041DOI Listing
July 2021

Thermodynamics Controlled Sharp Transformation from InP to GaP Nanowires via Introducing Trace Amount of Gallium.

Nanoscale Res Lett 2021 Mar 20;16(1):49. Epub 2021 Mar 20.

State Key Laboratory of Powder Metallurgy, Central South University, Changsha, 410083, China.

Growth of high-quality III-V nanowires at a low cost for optoelectronic and electronic applications is a long-term pursuit of research. Still, controlled synthesis of III-V nanowires using chemical vapor deposition method is challenge and lack theory guidance. Here, we show the growth of InP and GaP nanowires in a large area with a high density using a vacuum chemical vapor deposition method. It is revealed that high growth temperature is required to avoid oxide formation and increase the crystal purity of InP nanowires. Introduction of a small amount of Ga into the reactor leads to the formation of GaP nanowires instead of ternary InGaP nanowires. Thermodynamic calculation within the calculation of phase diagrams (CALPHAD) approach is applied to explain this novel growth phenomenon. Composition and driving force calculations of the solidification process demonstrate that only 1 at.% of Ga in the catalyst is enough to tune the nanowire formation from InP to GaP, since GaP nucleation shows a much larger driving force. The combined thermodynamic studies together with III-V nanowire growth studies provide an excellent example to guide the nanowire growth.
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http://dx.doi.org/10.1186/s11671-021-03505-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981363PMC
March 2021

Immune-related gene expression in the kidneys and spleens of goslings infected with goose nephritic astrovirus.

Poult Sci 2021 Apr 19;100(4):100990. Epub 2021 Jan 19.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Goose nephritic astrovirus (GNAstV) was first isolated in 2018, causing great economic losses to the goose industry. However, little is known about host immune response to GNAstV infection. In this study, forty 2-day-old goslings were randomly divided into 2 groups: infection and negative control groups. Each gosling in the infection group was challenged with 0.5 mL GNAstV-JSHA intramuscularly, whereas the gosling in the negative control group was inoculated with the same amount of PBS. Histopathological changes and virus location in the spleen and kidney were examined, and the expression of immune-related genes was determined by qPCR at 7 and 14 d after infection. Our results showed that GNAstV infection induced degeneration and necrosis of splenic lymphocytes and renal epithelial cells, and these cells were positive for the virus. In addition, GNAstV infection induced the activation of pattern recognition receptors (RIG-I, MDA-5, and TLR3) and key adaptor molecules (MyD88, MAVS, and IRF7) in the spleen and kidney, and upregulated the gene expression of interferon-α in the spleen and antiviral proteins (MX1, OASL, and IFITM3) in the spleen and kidney. Moreover, high expression levels of interleukin (IL)-1β and IL-8 in the spleen and iNOS in the spleen and kidney were found. These results indicated that GNAstV infection activated host innate immune response. Furthermore, GNAstV infection increased the expression levels of CD8, MHCI, and MHCII, indicating that adaptive immune response was activated. Besides, TGF-β was highly expressed in the spleen and kidney, which may be an immune evasion strategy of GNAstV to cause infection. Interestingly, both IL-1β and IL-6 mRNA levels were decreased in the kidney, which may help reduce kidney lesions. This is the first study to report changes in immune-related gene expression in response to GNAstV infection, and our results provide insights into viral pathogenesis.
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http://dx.doi.org/10.1016/j.psj.2021.01.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921877PMC
April 2021

Characterizing the Structural Pattern of Heavy Smokers Using Multivoxel Pattern Analysis.

Front Psychiatry 2020 4;11:607003. Epub 2021 Feb 4.

Department of Radiology, Shenzhen University General Hospital, Shenzhen, China.

Smoking addiction is a major public health issue which causes a series of chronic diseases and mortalities worldwide. We aimed to explore the most discriminative gray matter regions between heavy smokers and healthy controls with a data-driven multivoxel pattern analysis technique, and to explore the methodological differences between multivoxel pattern analysis and voxel-based morphometry. Traditional voxel-based morphometry has continuously contributed to finding smoking addiction-related regions on structural magnetic resonance imaging. However, voxel-based morphometry has its inherent limitations. In this study, a multivoxel pattern analysis using a searchlight algorithm and support vector machine was applied on structural magnetic resonance imaging to identify the spatial pattern of gray matter volume in heavy smokers. Our proposed method yielded a voxel-wise accuracy of at least 81% for classifying heavy smokers from healthy controls. The identified regions were primarily located at the temporal cortex and prefrontal cortex, occipital cortex, thalamus (bilateral), insula (left), anterior and median cingulate gyri, and precuneus (left). Our results suggested that several regions, which were seldomly reported in voxel-based morphometry analysis, might be latently correlated with smoking addiction. Such findings might provide insights for understanding the mechanism of chronic smoking and the creation of effective cessation treatment. Multivoxel pattern analysis can be efficient in locating brain discriminative regions which were neglected by voxel-based morphometry.
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http://dx.doi.org/10.3389/fpsyt.2020.607003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890259PMC
February 2021

Initiating Ullmann-like coupling of BrPy by a semimetal surface.

Sci Rep 2021 Feb 9;11(1):3414. Epub 2021 Feb 9.

Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201000, China.

Intensive efforts have been devoted to surface Ullmann-like coupling in recent years, due to its appealing success towards on-surface synthesis of tailor-made nanostructures. While attentions were mostly drawn on metallic substrates, however, Ullmann dehalogenation and coupling reaction on semimetal surfaces has been seldom addressed. Herein, we demonstrate the self-assembly of 2, 7-dibromopyrene (BrPy) and the well controllable dehalogenation reaction of BrPy on the Bi(111)-Ag substrate with a combination of scanning tunnelling microscopy (STM) and density functional theory calculations (DFT). By elaborately investigating the reaction path and formed organic nanostructures, it is revealed that the pristinely inert bismuth layer supported on the silver substrate can initiate Ullmann-like coupling in a desired manner by getting alloyed with Ag atoms underneath, while side products have not been discovered. By clarifying the pristine nature of Bi-Ag(111) and Ullmann-like reaction mechanisms, our report proposes an ideal template for thoroughly exploring dehalogenative coupling reaction mechanisms with atomic insights and on-surface synthesis of carbon-based architectures.
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http://dx.doi.org/10.1038/s41598-021-82973-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873249PMC
February 2021

Biodegradable Charge-Transfer Complexes for Glutathione Depletion Induced Ferroptosis and NIR-II Photoacoustic Imaging Guided Cancer Photothermal Therapy.

Angew Chem Int Ed Engl 2021 04 4;60(15):8157-8163. Epub 2021 Mar 4.

Institute of Advanced Materials and Flexible Electronics (IAMFE), School of Chemistry and Materials Science, Nanjing University of Information Science & Technology, Nanjing, 210044, China.

Suffering from the laborious synthesis and undesirable tumor microenvironment response, the exploitation of novel NIR-II absorbing organic photothermal agents is of importance to promote phototherapeutic efficacy. Herein, two kinds of charge-transfer complex nanoparticles (TMB-F4TCNQ and TMB-TCNQ) are prepared by supramolecular assembly. Because of the larger energy gap between donor and acceptor, TMB-F4TCNQ presents higher charge-transfer degree (72 %) than that of TMB-TCNQ (48 %) in nanoaggregates. Therefore, TMB-F4TCNQ exhibits stronger NIR-II absorption ability with a mass extinction coefficient of 15.4 Lg  cm at 1300 nm and excellent photothermal effect. Impressively, the specific cysteine response can make the TMB-F4TCNQ effectively inhibit the intracellular biosynthesis of GSH, leading to redox dsyhomeostasis and ROS-mediated ferroptosis. TMB-F4TCNQ can serve as a contrast agent for NIR-II photoacoustic imaging to guide precise and efficient photothermal therapy in vivo.
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http://dx.doi.org/10.1002/anie.202014852DOI Listing
April 2021

Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat Model.

Antioxidants (Basel) 2020 Dec 23;10(1). Epub 2020 Dec 23.

Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, Taiwan.

(-)-Epigallocatechin 3-gallate (EGCG) is the main active green tea catechin and has a wide variety of benefits for health. Post-traumatic osteoarthritis (PTOA) occurs as a consequence of joint injuries that commonly happen in the young population. In this study, we investigated the effects of EGCG on PTOA prevention by using the anterior cruciate ligament transection (ACLT)-OA model and further investigated the roles of autophagy in OA treatment. Our results showed that intra-articular injection of EGCG significantly improved the functional performances and decreased cartilage degradation. EGCG treatment attenuated the inflammation on synovial tissue and cartilage through less immunostained cyclooxygenase-2 and matrix metalloproteinase-13. We further noted EGCG may modulate the chondrocyte apoptosis by activation of the cytoprotective autophagy through reducing the expression of the mTOR and enhancing the expression of microtubule-associated protein light chain 3, beclin-1, and p62. In conclusion, intra-articular injection of EGCG after ACL injury inhibited the joint inflammation and cartilage degradation, thereby increasing joint function. EGCG treatment also reduced the chondrocyte apoptosis, possibly by activating autophagy. These findings suggested that EGCG may be a potential disease-modifying drug for preventing OA progression.
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http://dx.doi.org/10.3390/antiox10010008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824012PMC
December 2020

Phase Engineering of Epitaxial Stanene on a Surface Alloy.

J Phys Chem Lett 2021 Jan 16;12(1):211-217. Epub 2020 Dec 16.

School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Stanene is a notable two-dimensional topological insulator with a large spin-orbit-coupling-induced band gap. However, the formation of surface alloy intermediates during the epitaxial growth on noble metal substrates prevents the as-grown stanene from preserving its intrinsic electronic states. Here, we show that an intentionally prepared AuSn(111) alloy surface is a suitable inert substrate for growing stanene without the further formation of a complicated surface alloy by scanning tunneling microscopy. The Sn tetramer and clover-shaped Sn pentamer are intermediates for the black-phosphorene-like Sn film at a substrate temperature of <420 K, which transforms to a blue-phosphorene-like stanene with a lattice constant of 0.50 nm above 500 K. First-principles calculations reveal that the epitaxial Sn layer exhibits a lattice registry growth mode and holds a direct energy gap of ∼0.4 eV. Furthermore, interfacial charge-transfer-induced significant Rashba splitting in its electronic structure gives it great potential in spintronic applications.
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http://dx.doi.org/10.1021/acs.jpclett.0c03311DOI Listing
January 2021

[Detection of respiratory pathogens and clinical features in 225 children with acute exacerbation of bronchial asthma].

Zhongguo Dang Dai Er Ke Za Zhi 2020 Nov;22(11):1198-1203

Children's Medical Center, Hunan Provincial People's Hospital, Changsha 410002, China.

Objective: To investigate the respiratory pathogens and clinical features in children with acute exacerbation of bronchial asthma.

Methods: Nasopharyngeal swabs were collected from 225 children with acute exacerbation of bronchial asthma, aged <14 years, who attended the outpatient service or were hospitalized from August 2017 to August 2019. Quantitative real-time PCR was used to detect 12 pathogens, i.e., respiratory syncytial virus (RSV), human rhinovirus (HRV), influenza virus A (IFVA), influenza virus B (IFVB), parainfluenza virus types 1-3 (PIV1-3), human metapneumovirus (HMPV), adenovirus (ADV), Bordetella pertussis (BP), Chlamydia pneumoniae (CP), and Mycoplasma pneumoniae (MP).

Results: The overall detection rate of virus was 46.2% (104/225), and 7 kinds of viruses were detected, i.e., HRV (19.6%, 44/225), ADV (16.0%, 36/225), IFVB (5.8%, 13/225), RSV (4.9%, 11/225), IFVA (3.6%, 8/225), PIV3 (1.8%, 4/225), and HMPV (0.4%, 1/225). Of all pathogens, BP had the highest detection rate of 28.4% (64/225), and the detection rates of MP and CP were 16.4% (37/225) and 0.4% (1/225), respectively. The mild exacerbation group had a higher detection rate of BP than the severe exacerbation group (P<0.05), while the severe exacerbation group had significantly higher detection rates of RSV and MP than the mild exacerbation group (P<0.05). There were significant differences in the proportion of children with paroxysmal cough, spasmodic cough, fever, lung rales and abnormal lung imaging findings among the simple BP infection, simple virus infection and simple MP infection groups (P<0.05).

Conclusions: BP, HRV, and MP are common respiratory pathogens detected in children with acute exacerbation of bronchial asthma, and respiratory virus infection is an important pathogen of acute exacerbation of asthma in children. Acute exacerbation of asthma caused by different pathogens has different clinical features and severities.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666394PMC
November 2020

Modification of an ultrathin C interlayer on the electronic structure and molecular packing of C8-BTBT on HOPG.

Phys Chem Chem Phys 2020 Nov 2;22(43):25264-25271. Epub 2020 Nov 2.

School of Physics and Electronics, Central South University, Changsha, 410083, P. R. China.

X-ray photoelectron spectroscopy (XPS), ultraviolet photoelectron spectroscopy (UPS), atomic force microscopy (AFM) and X-ray diffraction (XRD) were applied to investigate the electronic structure and molecular packing of C8-BTBT on HOPG with an ultrathin C interlayer. It was found that C8-BTBT displays a Vollmer-Weber (V-W) growth mode on HOPG, with an ultrathin C interlayer (0.7 nm). Compared to the uniform lying-down growth mode as directly grown on HOPG, the C8-BTBT molecules here adopt a lying-down orientation at low coverage with some small tilt angles because the π-π interaction between C8-BTBT and HOPG is partly disturbed by the C interlayer, delivering a higher highest occupied molecular orbital (HOMO) in C8-BTBT. An interface dipole of 0.14 eV is observed due to electron transport from C8-BTBT to C. The upward and downward band bending in C8-BTBT and C, respectively, near the C8-BTBT/C interface reduces the hole transport barrier at the interface, facilitating the hole injection from C to C8-BTBT, while a large electron transfer barrier from C to C8-BTBT is detected at this interface, which effectively limits electron injection from C to C8-BTBT. The HOMO of C8-BTBT near the interface is largely lifted up by the C insertion layer, which causes a p-doping effect and increases the hole mobility in C8-BTBT. Furthermore, owing to the lowest occupied molecular orbital (LUMO) of C residing in the gap of C8-BTBT, charge transfer occurs between C and the trap states in C8-BTBT to effectively passivate the trapping states. Our efforts aid a better understanding of the electron structure and film growth of anisotropic molecules and provide a useful strategy to improve the performance of C8-BTBT-based devices.
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http://dx.doi.org/10.1039/d0cp04288aDOI Listing
November 2020

A comparative study on the dielectric response and microwave absorption performance of FeNi-capped carbon nanotubes and FeNi-cored carbon nanoparticles.

Nanotechnology 2020 Dec 10;32(10). Epub 2020 Dec 10.

School of Mechanical and Mining Engineering, The University of Queensland, Brisbane, QLD 4072, Australia.

The mechanisms responsible for the dielectric response of C-based microwave absorbers remain a long-standing theoretical question. Uncovering these mechanisms is critical to enhance their microwave absorption performance. To determine how different C forms alter the dielectric response of C-based absorbers, FeNi-capped carbon nanotubes (FeNi-CNTs) and FeNi-cored carbon nanoparticles (FeNi-CNPs) are synthesized, and a comparative study of their dielectric responses is then carried out in this study. The as-synthesized FeNi-CNTs and FeNi-CNPs have similar magnetic properties and complex permeabilities, but differ in complex permittivities. It is shown that FeNi-CNTs have a much stronger dielectric loss than FeNi-CNPs. At a thickness of 2.8 mm, a low optimal reflection loss of -32.2 dB and a broad effective absorption bandwidth of 8.0 GHz are achieved for FeNi-CNTs. Meanwhile, equivalent circuit models reveal that the CNT network of the FeNi-CNTs could introduce an electrical inductance that can effectively improve its dielectric loss capability. This study demonstrates that designing a composite with a tailored C form and composition is a successful strategy for tuning its microwave absorption performance. Furthermore, the equivalent circuit modeling is an effective tool for analyzing the dielectric response of the microwave absorbers, as is expected to be applicable for other metal-C composites.
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http://dx.doi.org/10.1088/1361-6528/abc644DOI Listing
December 2020

USF3 modulates osteoporosis risk by targeting WNT16, RANKL, RUNX2, and two GWAS lead SNPs rs2908007 and rs4531631.

Hum Mutat 2021 Jan 30;42(1):37-49. Epub 2020 Nov 30.

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China.

Osteoporotic fractures cause major morbidity and mortality in the aging population. Genome-wide association studies (GWAS) have identified USF3 as the novel susceptibility gene of osteoporosis. However, the functional role in bone metabolism and the target gene of the basic helix-loop-helix transcription factor USF3 are unclear. Here, we show that USF3 enhances osteoblast differentiation and suppresses osteoclastogenesis in cultured human osteoblast-like U-2OS cells. Mechanistic studies revealed that transcription factor USF3 antagonistically interacts with anti-osteogenic TWIST1/TCF12 heterodimer in the WNT16 and RUNX2 promoter, and counteracts CREB1 and JUN/FOS in the RANKL promoter. Importantly, the osteoporosis GWAS variant g.1744A>G (rs2908007A>G) located in the WNT16 promoter confers G-allele-specific transcriptional modulation by USF3, TWIST1/TCF12 and TBX5/TBX15, and USF3 transactivates the osteoclastogenesis suppressor WNT16 promoter activity and antagonizes the repression of WNT16 by TWIST1 and TCF12. The risk G allele of osteoporosis GWAS variant g.3260A>G (rs4531631A>G) in the RANKL promoter facilitates the binding of CREB1 and JUN/FOS and enhances transactivation of the osteoclastogenesis contributor RANKL that is inhibited by USF3. Our findings uncovered the functional mechanisms of osteoporosis novel GWAS-associated gene USF3 and lead single nucleotide polymorphisms rs2908007 and rs4531631 in the regulation of bone formation and resorption.
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http://dx.doi.org/10.1002/humu.24126DOI Listing
January 2021

Vertical 0D-Perovskite/2D-MoS van der Waals Heterojunction Phototransistor for Emulating Photoelectric-Synergistically Classical Pavlovian Conditioning and Neural Coding Dynamics.

Small 2020 11 9;16(45):e2005217. Epub 2020 Oct 9.

Hunan Key Laboratory of Super Microstructure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha, Hunan, 410083, China.

Optoelectronic-neuromorphic transistors are vital for next-generation nanoscale brain-like computational systems. However, the hardware implementation of optoelectronic-neuromorphic devices, which are based on conventional transistor architecture, faces serious challenges with respect to the synchronous processing of photoelectric information. This is because mono-semiconductor material cannot absorb adequate light to ensure efficient light-matter interactions. In this work, a novel neuromorphic-photoelectric device of vertical van der Waals heterojunction phototransistors based on a colloidal 0D-CsPbBr -quantum-dots/2D-MoS heterojunction channel is proposed using a polymer ion gel electrolyte as the gate dielectric. A highly efficient photocarrier transport interface is established by introducing colloidal perovskite quantum dots with excellent light absorption capabilities on the 2D-layered MoS semiconductor with strong carrier transport abilities. The device exhibits not only high photoresponsivity but also fundamental synaptic characteristics, such as excitatory postsynaptic current, paired-pulse facilitation, dynamic temporal filter, and light-tunable synaptic plasticity. More importantly, efficiency-adjustable photoelectronic Pavlovian conditioning and photoelectronic hybrid neuronal coding behaviors can be successfully implemented using the optical and electrical synergy approach. The results suggest that the proposed device has potential for applications associated with next-generation brain-like photoelectronic human-computer interactions and cognitive systems.
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http://dx.doi.org/10.1002/smll.202005217DOI Listing
November 2020

The adhesion of a mica nanolayer on a single-layer graphene supported by SiO substrate characterised in air.

Nanotechnology 2021 Jan;32(4):045701

School of Mechanical and Mining Engineering, The University of Queensland, Brisbane QLD, 4072, Australia.

Two-dimensional nanolayers have found increasingly widespread applications in modern flexible electronic devices. Their adhesion with neighbouring layers can significantly affect the mechanical stability and the reliability of those devices. However, the measurement of such adhesion has been a great challenge. In this work, we develop a new and simple methodology to measure the interfacial adhesion between a mica nanolayer (MNL) and a single-layer graphene (SLG) supported by a SiO substrate. The method is based on the well-known Obreimoff method but integrated with innovative nanomanipulation and profile measuring approaches. Our study shows that the adhesion energy of MNLs on the SLG/SiO substrate system is considerably lower than that on the SiO substrate alone. Quantitative analyses reveal that the wrinkles formed on the SLG can considerably lower the adhesion. This outcome is of technological value as the adhesion maybe tailored by controlling the wrinkle formation in the graphene layer in a flexible electronic device.
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http://dx.doi.org/10.1088/1361-6528/abbf25DOI Listing
January 2021
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