Publications by authors named "Han Hu"

238 Publications

Spatial spillover effects of urbanization on carbon emissions in the Yangtze River Delta urban agglomeration, China.

Environ Sci Pollut Res Int 2022 Jan 15. Epub 2022 Jan 15.

School of Tourism and Urban Management, Jiangxi University of Finance and Economics, Nanchang, 330013, China.

To achieve a win-win situation for both urbanization and carbon emissions reduction from a spatiotemporal perspective, we need to identify the salient links between urbanization and carbon emissions in different dimensions. Using 2008-2018 panel data on the Yangtze River Delta urban agglomeration, this paper constructs a Stochastic Impacts by Regression on Population, Affluence, and Technology (STIRPAT) model based on four dimensions of urbanization: population, economy, land, and ecology. Additionally, it uses a whole group of variables for reference, constructs a Spatial Durbin model (SDM) to estimate the spatial effect, and empirically investigates the spatial dependence of carbon emissions and the influence of various driving factors. The results show that (1) in the temporal dimension, the historical carbon emissions of the study area continue to increase. However, the extent to which they are doing so is slowing, the number of low carbon emissions areas has significantly decreased, the number of medium carbon emissions areas have significantly increased, the number of high and relatively high carbon emissions areas are relatively stable, and energy intensity continues to decline. (2) In the spatial dimension, Shanghai, Suzhou, and their surrounding cities have always been carbon emissions hotspots, high and relatively high carbon emissions areas are mainly concentrated in these cities. Low carbon emissions areas and cold spots are mainly distributed in Anhui Province. Medium carbon emissions areas show a great spatial and temporal evolution and are distributed in all provinces. (3) In the four dimensions of urbanization, per capita GDP will not only affect regional carbon emissions but also have a spatial spillover effect. For every 1% increase in the economic factors, carbon emissions in neighboring regions will increase by 0.38-0.43%. Population, economic, and technological factors have significant positive effects on carbon emissions, and economic factor is the most important factor. (4) In different dimensions of urbanization, there are obvious heterogeneities in the impacts of different factors on carbon emissions. Among them, the elasticity coefficient of per capita GDP and energy intensity is the smallest among the dimension of land urbanization, and the elasticity coefficient of the total population is the smallest among the dimension of population urbanization. Therefore, when formulating carbon emissions reduction policies, it is necessary to fully consider the spatial spillover effects, determine the optimal population size threshold, advocate for a low-carbon lifestyle, promote clean technology, and realize information exchange and policy interaction across regions from the perspective of holistic governance.
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http://dx.doi.org/10.1007/s11356-021-17872-xDOI Listing
January 2022

Novel fusion peptides deliver exosomes to modify injectable thermo-sensitive hydrogels for bone regeneration.

Mater Today Bio 2022 Jan 27;13:100195. Epub 2021 Dec 27.

School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin, 300070, China.

Injectable thermo-sensitive hydrogels composed of small intestinal submucosa (SIS) with exosomes derived from bone marrow mesenchymal stem cells (BMSCs) are desired for bone regeneration. However, poor mechanical properties limit the clinical application of SIS hydrogels. Herein, the mechanical properties of SIS hydrogels incorporated with 3-(3,4-dihydroxyphenyl) propionic acid (CA) are assessed. The results show that the mechanical properties of SIS hydrogels are improved. In addition, the retention and stability of exosomes over time at the defect site are also challenges. Fusion peptides are designed by connecting collagen-binding domines (CBDs) of collagen type I/III with exosomal capture peptides CP05 (CRHSQMTVTSRL) directly or via rigid linkers (EAAAK). In vitro experiments demonstrate that fusion peptides are contribute to promoting the positive effect of exosomes on osteogenic differentiation of BMSCs. Meanwhile, the results of hydrogels combining exosomes and fusion peptides in the treatment of rat skull defect models reveal that fusion peptides could enhance the retention and stability of exosomes, thereby strengthen the therapeutic effect for skull defects. Therefore, SIS hydrogels with CA modified by fusion peptides and exosomes appear to be a promising strategy in bone regenerative medicine.
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http://dx.doi.org/10.1016/j.mtbio.2021.100195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724941PMC
January 2022

Tumor acidity-activatable photothermal/Fenton nanoagent for synergistic therapy.

J Colloid Interface Sci 2021 Dec 25;612:355-366. Epub 2021 Dec 25.

Department of Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong SAR; State Key Laboratory of Terahertz and Millimeter Waves, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong SAR. Electronic address:

Intracellular formation of therapeutic agents has become one of the effective ways for cancer-specific treatment. Herein, a tumor acidity-activatable photothermal/Fenton nanoagent (denoted as CoPy) was constructed based on oxidized zeolitic imidazolate framework-67 (oxZIF-67) nanosheet and pyrrole (Py) monomer for synergistic therapy. The CoPy showed negligible toxicity to normal cell models RAW264.7 and 3T3 cell lines, and could be degraded by ascorbic acid in normal physiological conditions. However, once uptaken by 4T1 cells, the acidic pH led to the release of Co, which served as a strong oxidant to induce the polymerization of Py to form polypyrrole (PPy) for site-specific photothermal therapy (PTT). Most appealingly, the PPy could chelate the generated Co in the polymerization process to initiate the Fenton-like reaction, which was more capable to produce highly toxic hydroxyl radical (•OH) for chemodynamic therapy (CDT) compared to the free Co ones. In vitro and in vivo experiments demonstrated that all functionalities on CoPy worked collaboratively, and 78% of tumors were inhibited through cooperative PTT/CDT. Such a novel therapeutic nanoagent with tumor selectivity opens new opportunities for combinational treatment paradigms.
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http://dx.doi.org/10.1016/j.jcis.2021.12.134DOI Listing
December 2021

Multifunctional phototheranostic nanoplatform based on polydopamine-manganese dioxide-IR780 iodide for effective magnetic resonance imaging-guided synergistic photodynamic/photothermal therapy.

J Colloid Interface Sci 2022 Apr 13;611:193-204. Epub 2021 Dec 13.

Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

Multifunctional phototheranostics combining diagnostic and therapeutic modalities may provide a revolutionary opportunity for cancer treatment. As a promising tumor phototheranostic molecule, IR780 iodide (IR780) shows excellent photodynamic and photothermal performance under near-infrared laser irradiation; however, its hydrophobicity and instability limit its further use in organisms. This work demonstrates the design and development of a multifunctional nanoplatform (PMIDA, referring to polydopamine (PDA)-manganese dioxide (MnO)-IR780) for imaging-guided phototherapy. The good biocompatibility of PDA greatly improves the water solubility and photostability of IR780, and its excellent photothermal properties make PMIDA a dual photothermal therapy (PTT). MnO-induced generation of oxygen in the tumor microenvironment improves the hypoxia effect and photodynamic therapy (PDT) of IR780. Moreover, Mn serves as a decent T-weighted magnetic resonance imaging (MRI) probe to guide treatment. Notably, in relevant cellular assays, PMIDA shows high photodynamic and photothermal effects contributing to the final therapeutic effect. The MRI-guided PDT/PTT synergistic therapy effect in vivo is demonstrated by precise tumor diagnosis and complete tumor elimination outcomes. Based on these experiments, PMIDA nanoparticles display promising effects in facilitating intravenous injection of IR780 and achieving magnetic resonance imaging (MRI)-guided phototheranostic efficacy for tumor treatment.
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http://dx.doi.org/10.1016/j.jcis.2021.12.071DOI Listing
April 2022

Design of 2,5-furandicarboxylic based polyesters degraded in different environmental conditions: Comprehensive experimental and theoretical study.

J Hazard Mater 2022 Mar 30;425:127752. Epub 2021 Nov 30.

Key Laboratory of Bio-based Polymeric Materials Technology and Application of Zhejiang Province, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China. Electronic address:

Nowadays, the promotion and application of aliphatic-aromatic copolyesters, such as poly (butylene adipate-co-terephthalate) (PBAT), are growing into a general trend. Although the structures of diacids exerted substantial impacts on degradation behavior, the underlying mechanisms have rarely been studied. In this work, 2,5-Furandicarboxylic acid was combined with succinic acid (PBSF), adipic acid (PBAF) and diglycolic acid (PBDF) to prepare three kinds of copolyesters. They showed unique degradation behaviors in buffer, enzyme environment and artificial seawater. These characteristics are closely related to the structural compositions of diacids. PBAFs displayed impressive biodegradability when catalyzed by Candida antarctica lipase B (CALB), while the more hydrophilic PBDFs exhibited faster hydrolysis in both buffer and artificial seawater. PBSFs, with hydrophobic and short segments, obtained a relatively slower rate of hydrolysis and enzymatic degradation. The reactivity sites and hydrolytic pathway were revealed by the combination of DFT calculation and Fukui function analysis. MD simulations, QM/MM optimizations and theozyme calculations showed that PBAF-CALB was prone to form a pre-reaction state, leading to the reduced energy barrier in the acylation process. This work revealed the effects of different structural features of diacids on polymer degradation and paved a way to design target biodegradable polymers in different degradation conditions.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127752DOI Listing
March 2022

Research on the Interaction of Genetic Algorithm in Assisted Composition.

Authors:
Han Hu

Comput Intell Neurosci 2021 22;2021:3137666. Epub 2021 Nov 22.

School of Architecture and Art, Central South University, Changsha, Hunan 410006, China.

Computer-aided composition is an attempt to use a formalized process to minimize human (or composer) involvement in the creation of music using a computer. Exploring the problem of computer-aided composition can enable us to understand and simulate the thinking mode of composers in the special process of music creation, which is an important application of artificial intelligence in the field of art. Feature extraction on the MIDI files has been introduced in this paper. Based on the genetic algorithm in this paper, a platform of the sampling coding method to optimize the character representation has solved the traditional algorithmic music composition study. Music directly from the pitch and duration can be derived from the characteristics, respectively, in the form of a one-hot encoding independently said. Failure to the rhythm of the characterization of the pitch and duration are problems that lead to the inability of compositional networks to learn musical styles better. Rhythm is the combination of pitch and time values according to certain rules. The rhythm of music affects the overall style of music. By associating the pitch and time value coding, the rhythm style of music can be preserved better so that the composition network can learn the style characteristics of music more easily.
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http://dx.doi.org/10.1155/2021/3137666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629674PMC
December 2021

Lenvatinib and CuS nanocrystals co-encapsulated in poly(D,L-lactide--glycolide) for synergistic chemo-photothermal therapy against advanced hepatocellular carcinoma.

J Mater Chem B 2021 12 15;9(48):9908-9922. Epub 2021 Dec 15.

Cancer Center, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei, 430022, China.

Lenvatinib (LT) is gradually replacing sorafenib as an alternative targeted drug against advanced hepatocellular carcinoma (HCC). However, the anticancer effects of LT are still limited because of its low cytotoxicity, multidrug resistance (MDR), and tumor relapse. Herein, we constructed a smart biophotonic nanoplatform to overcome the barriers preventing high performance. LT and copper sulfide nanocrystals (CuS NCs) with excellent photothermal properties in the near-infrared-II (NIR-II) zone were co-encapsulated in poly(D,L-lactide--glycolide) (PLGA) through nanoprecipitation. Both and evaluations demonstrated that CuS NCs enhanced the anticancer efficacy of LT, without recurrence. In addition, the presence of copper ions could allow glutathione (GHS) to be consumed and oxygen to be produced, likely suppressing the expression of P-glycoprotein (P-gp) and overcoming the issue of MDR relating to LT. More importantly, synergistic chemo-photothermal therapy with LT and CuS NCs was more effective than any single therapy or theoretical combination. This nanoplatform is promising for advancing future LT-based treatment strategies for HCC therapy.
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http://dx.doi.org/10.1039/d1tb01808fDOI Listing
December 2021

Research on Task-Service Network Node Matching Method Based on Multi-Objective Optimization Model in Dynamic Hyper-Network Environment.

Micromachines (Basel) 2021 Nov 21;12(11). Epub 2021 Nov 21.

Anyang Institute of Technology, College of Mechanical Engineering, Anyang 455000, China.

In order to reduce the cost of manufacturing and service for the Cloud 3D printing (C3DP) manufacturing grid, to solve the problem of resources optimization deployment for no-need preference under circumstance of cloud manufacturing, consider the interests of enterprises which need Cloud 3D printing resources and cloud platform operators, together with QoS and flexibility of both sides in the process of Cloud 3D printing resources configuration service, a task-service network node matching method based on Multi-Objective optimization model in dynamic hyper-network environment is built for resource allocation. This model represents interests of the above-mentioned two parties. In addition, the model examples are solved by modifying Mathematical algorithm of Node Matching and Evolutionary Solutions. Results prove that the model and the algorithm are feasible, effective and stable.
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http://dx.doi.org/10.3390/mi12111427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624609PMC
November 2021

Flexural Behavior of Two-Span Continuous CFRP RC Beams.

Materials (Basel) 2021 Nov 9;14(22). Epub 2021 Nov 9.

Hubei Key Laboratory of Roadway Bridge & Structure Engineering, Wuhan University of Technology, Wuhan 430070, China.

This paper investigates the feasibility of replacing steel bars with carbon-fiber-reinforced polymer (CFRP) bars in continuous reinforced concrete (RC) beams. A numerical model is introduced. Model predictions are compared with the experimental results that are available in the literature. A comprehensive numerical investigation is then performed on two-span CFRP/steel RC beams with = 0.61-3.03% and / = 1.5, where and are tensile bar ratios (ratios of tensile bar area to effective cross-sectional area of beams) over positive and negative moment regions, respectively. The study shows that replacing steel bars with CFRP bars greatly improves the crack mode at a low bar ratio. The ultimate load of CFRP RC beams is 89% higher at = 0.61% but 7.2% lower at = 3.03% than that of steel RC beams. In addition, CFRP RC beams exhibit around 13% greater ultimate deflection compared to steel RC beams. The difference of moment redistribution between CFRP and steel RC beams diminishes as increases. ACI 318-19 appears to be conservative, and it leads to more accurate predictions of moment redistribution in CFRP RC beams than that in steel RC beams.
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http://dx.doi.org/10.3390/ma14226746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622512PMC
November 2021

Responses of AM fungal abundance to the drivers of global climate change: A meta-analysis.

Sci Total Environ 2022 Jan 16;805:150362. Epub 2021 Sep 16.

Shaanxi Key Laboratory of Earth Surface System and Environmental Carrying Capacity, Northwest University, Xi'an 710127, Shaanxi, China; College of Urban and Environmental Sciences, Northwest University, Xi'an 710127, Shaanxi, China. Electronic address:

Arbuscular mycorrhizal fungi (AMF), playing critical roles in carbon cycling, are vulnerable to climate change. However, the responses of AM fungal abundance to climate change are unclear. A global-scale meta-analysis was conducted to investigate the response patterns of AM fungal abundance to warming, elevated CO concentration (eCO), and N addition. Both warming and eCO significantly stimulated AM fungal abundance by 18.6% (95%CI: 5.9%-32.8%) and 21.4% (15.1%-28.1%) on a global scale, respectively. However, the response ratios (RR) of AM fungal abundance decreased with the degree of warming while increased with the degree of eCO. Furthermore, in warming experiments, as long as the warming exceeded 4 °C, its effects on AM fungal abundance changed from positive to negative regardless of the experimental durations, methods, periods, and ecosystem types. The effects of N addition on AM fungal abundance are -5.4% (-10.6%-0.2%), and related to the nitrogen fertilizer input rate and ecosystem type. The RR of AM fungal abundance is negative in grasslands and farmlands when the degree of N addition exceeds 33.85 and 67.64 kg N ha yr, respectively; however, N addition decreases AM fungal abundance in forests only when the degree of N addition exceeds 871.31 kg N ha yr. The above results provide an insight into predicting ecological functions of AM fungal abundance under global changes.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150362DOI Listing
January 2022

NK cell tumor therapy modulated by UV-inactivated oncolytic herpes simplex virus type 2 and checkpoint inhibitors.

Transl Res 2022 Feb 29;240:64-86. Epub 2021 Oct 29.

National "111" Centre for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Centre of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China. Electronic address:

Oncolytic virotherapy is a new and safe therapeutic strategy for cancer treatment. In our previous study, a new type of oncolytic herpes simplex virus type 2 (oHSV2) was constructed. Following the completion of a preclinical study, oHSV2 has now entered into clinical trials for the treatment of melanoma and other solid tumors (NCT03866525). Oncolytic viruses (OVs) are generally able to directly destroy tumor cells and stimulate the immune system to fight tumors. Natural killer (NK) cells are important components of the innate immune system and critical players against tumor cells. But the detailed interactions between oncolytic viruses and NK cells and these interaction effects on the antitumor immune response remain to be elucidated. In particular, the functions of activating surface receptors and checkpoint inhibitors on oHSV2-treated NK cells and tumor cells are still unknown. In this study, we found that UV-oHSV2 potently activates human peripheral blood mononuclear cells, leading to increased antitumor activity in vitro and in vivo. Further investigation indicated that UV-oHSV2-stimulated NK cells release IFN-γ via Toll-like receptor 2 (TLR2)/NF-κB signaling pathway and exert antitumor activity via TLR2. We found for the first time that the expression of a pair of checkpoint molecules, NKG2A (on NK cells) and HLA-E (on tumor cells), is upregulated by UV-oHSV2 stimulation. Anti-NKG2A and anti-HLA-E treatment could further enhance the antitumor effects of UV-oHSV2-stimulated NK92 cells in vitro and in vivo. As our oHSV2 clinical trial is ongoing, we expect that the combination therapy of oncolytic virus oHSV2 and anti-NKG2A/anti-HLA-E antibodies may have synergistic antitumor effects in our future clinical trials.
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http://dx.doi.org/10.1016/j.trsl.2021.10.006DOI Listing
February 2022

Polycyclic Aromatic Hydrocarbons as a New Class of Promising Cathode Materials for Aluminum-Ion Batteries.

Angew Chem Int Ed Engl 2022 Jan 27;61(3):e202114681. Epub 2021 Nov 27.

State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao, 266580, P. R. China.

As an emerging post-lithium battery technology, aluminum ion batteries (AIBs) have the advantages of large Al reserves and high safety, and have great potential to be applied to power grid energy storage. But current graphite cathode materials are limited in charge storage capacity due to the formation of stage-4 graphite-intercalated compounds (GICs) in the fully charged state. Herein, we propose a new type of cathode materials for AIBs, namely polycyclic aromatic hydrocarbons (PAHs), which resemble graphite in terms of the large conjugated π bond, but do not form GICs in the charge process. Quantum chemistry calculations show that PAHs can bind AlCl through the interaction between the conjugated π bond in the PAHs and AlCl forming on-plane interactions. The theoretical specific capacity of PAHs is negatively correlated with the number of benzene rings in the PAHs. Then, under the guidance of theoretical calculations, anthracene, a three-ring PAH, was evaluated as a cathode material for AIBs. Electrochemical measurements show that anthracene has a high specific capacity of 157 mAh g (at 100 mA g ) and still maintains a specific capacity of 130 mAh g after 800 cycles. This work provides a feasible "theory guides practice" research model for the development of energy storage materials, and also provides a new class of promising cathode materials for AIBs.
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http://dx.doi.org/10.1002/anie.202114681DOI Listing
January 2022

Flexible electrodes with high areal capacity based on electrospun fiber mats.

Nanoscale 2021 Nov 18;13(44):18391-18409. Epub 2021 Nov 18.

State Key Laboratory of Heavy Oil Processing, Institute of New Energy, College of Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China.

The ever-growing portable, flexible, and wearable devices impose new requirements from power sources. In contrast to gravitational metrics, areal metrics are more reliable performance indicators of energy storage systems for portable and wearable devices. For energy storage devices with high areal metrics, a high mass loading of the active species is generally required, which imposes formidable challenges on the current electrode fabrication technology. In this regard, integrated electrodes made by electrospinning technology have attracted increasing attention due to their high controllability, excellent mechanical strength, and flexibility. In addition, electrospun electrodes avoid the use of current collectors, conductive additives, and polymer binders, which can essentially increase the content of the active species in the electrodes as well as reduce the unnecessary physically contacted interfaces. In this review, the electrospinning technology for fabricating flexible and high areal capacity electrodes is first highlighted by comparing with the typical methods for this purpose. Then, the principles of electrospinning technology and the recent progress of electrospun electrodes with high areal capacity and flexibility are elaborately discussed. Finally, we address the future perspectives for the construction of high areal capacity electrodes using electrospinning technology to meet the increasing demands of flexible energy storage systems.
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http://dx.doi.org/10.1039/d1nr05681fDOI Listing
November 2021

Phosphoproteomic basis of neuroplasticity in the antennal lobes influences the olfactory differences between A. mellifera and A. cerana honeybees.

J Proteomics 2022 Jan 30;251:104413. Epub 2021 Oct 30.

Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Science, Beijing 100093, China. Electronic address:

The honeybee species A. mellifera and A. cerana have evolved substantial differences in olfactory-driven behaviors and in peripheral olfactory systems. Knowledge of the central nervous system regulating these olfaction differences is limited, however. We compared the phosphoproteome of the antennal lobes (ALs, the primary olfactory neuropil) of A. mellifera and A. cerana, and identified a total of 2812 phosphopeptides carrying 2971 phosphosites from 1265 phosphoproteins. Of these, 76% of the phosphoproteins were shared by both species, which were mainly presynapse and cytoskeleton components, and were involved in signal transduction and neurotransmitter secretion. This finding indicates the fundamental role of protein phosphorylation in regulating signal transduction in the ALs. The mTOR signaling pathway, the phagosome pathway, and the autophagy pathway, which are important in protein metabolism, were enriched, suggesting glomeruli plasticity and olfactory processing are intensively modulated by phosphorylation via these pathways. Compared with A. mellifera, 107 phosphoproteins associated with protein metabolism and transport were uniquely expressed in A. cerana, indicating the protein synthesis-dependent synaptic plasticity is enhanced in A. cerana to facilitate the processing of more complex floral odor clues in mountain foraging areas. This finding is further supported by the significantly upregulated key phosphoproteins of the mTOR signaling pathway in A. cerana ALs. These results provide insights into the phosphoproteomic basis of neuroplasticity that is coupled with the divergent evolution of bees in different environments. SIGNIFICANCE: To adapt to their own ecological niche, the two major honeybee species, A. mellifera and A. cerana, have developed significant difference in olfactory-driven behaviors, but our understanding of the underlying regulation of the central nervous system is still limitate. Here we performed the first comprehensive phosphoproteomic comparison of antennal lobes (Als) between A. mellifera and A. cerena. A large proportion of the identified phosphosites and phosphoproteins were shared between the two species to serve as a core network in the regulation of signal transduction and glomeruli plasticity of ALs. However, compared with A. mellifera, 107 phosphoproteins associated with protein metabolism and transport were uniquely identified in A. cerana ALs, and also several key phosphoproteins in mTOR signaling pathway were found upregulated in A. cerana. These findings indicate protein phosphorylation enhanced the protein synthesis-dependent synaptic plasticity in A. cerana to facilitate the processing of more complex floral odor clues in mountain foraging areas. Our data provide a valuable insight into phosphoproteome-driven cerebral regulation of honeybee olfactory behaviors, which is potentially useful for further neurobiological investigation in both honeybees and other insects.
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http://dx.doi.org/10.1016/j.jprot.2021.104413DOI Listing
January 2022

Robust and Fast Lithium Storage Enabled by Polypyrrole-Coated Nitrogen and Phosphorus Co-Doped Hollow Carbon Nanospheres for Lithium-Ion Capacitors.

Front Chem 2021 24;9:760473. Epub 2021 Sep 24.

State Key Laboratory of Heavy Oil Processing, Institute of New Energy, College of Chemical Engineering, China University of Petroleum (East China), Qingdao, China.

Lithium-ion capacitors (LICs) have been proposed as an emerging technological innovation that integrates the advantages of lithium-ion batteries and supercapacitors. However, the high-power output of LICs still suffers from intractable challenges due to the sluggish reaction kinetics of battery-type anodes. Herein, polypyrrole-coated nitrogen and phosphorus co-doped hollow carbon nanospheres ([email protected]) were synthesized by a facile method and employed as anode materials for LICs. The unique hybrid architecture composed of porous hollow carbon nanospheres and PPy coating layer can expedite the mass/charge transport and enhance the structural stability during repetitive lithiation/delithiation process. The N and P dual doping plays a significant role on expanding the carbon layer spacing, enhancing electrode wettability, and increasing active sites for pseudocapacitive reactions. Benefiting from these merits, the [email protected] composite exhibits excellent lithium-storage performances including high rate capability and good cycling stability. Furthermore, a novel LIC device based on the [email protected] anode and the nitrogen-doped porous carbon cathode delivers a high energy density of 149 Wh kg and a high power density of 22,500 W kg as well as decent cycling stability with a capacity retention rate of 92% after 7,500 cycles. This work offers an applicable and alternative way for the development of high-performance LICs.
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http://dx.doi.org/10.3389/fchem.2021.760473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497749PMC
September 2021

Antioxidation and Energy-Level Alignment for Improving Efficiency and Stability of Hole Transport Layer-Free and Methylammonium-Free Tin-Lead Perovskite Solar Cells.

ACS Appl Mater Interfaces 2021 Sep 10;13(37):45059-45067. Epub 2021 Sep 10.

Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong 999077, SAR, China.

Tin-lead (Sn-Pb) perovskites have shown great potential in applications of single-junction perovskite solar cells (PSCs) and tandem devices due to outstanding photoelectrical properties and low band gaps. Currently, Sn-Pb PSCs typically have a p-i-n structure, but choices of hole transport layer (HTL) materials are very limited and there are different concerns in each of them. Eliminating the HTL is a direct and promising strategy to address the concerns, but is rarely studied. In this work, we demonstrate HTL-free and MA-free based Sn-Pb PSCs and a synergistic integration strategy of simultaneously introducing a reducing agent and in situ surface passivation. With the integration strategy, Sn-Pb perovskite films with enhanced antioxidation, reduced trap density, prolonged carrier lifetime, and improved energy-level alignment are achieved. Consequently, final HTL-free PSCs exhibit a champion power conversion efficiency (PCE) of 17.4%, which is a new record for HTL-free and MA-free Sn-Pb PSCs. Meanwhile, the integration strategy-based HTL-free device maintains excellent stability with efficiency unchanged for the first 200 h, and finally retaining 81% of the efficiency after 480 h aging in the air. This study shows the potential of achieving desirable HTL-free and MA-free Sn-Pb PSCs and offers more opportunities for tandem solar cells and other photovoltaic devices.
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http://dx.doi.org/10.1021/acsami.1c12180DOI Listing
September 2021

Development of a Freshness Assay for Royal Jelly Based on the Temperature- and Time-Dependent Changes of Antimicrobial Effectiveness and Proteome Dynamics of Royal Jelly Proteins.

J Agric Food Chem 2021 Sep 1;69(36):10731-10740. Epub 2021 Sep 1.

Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

Although the antimicrobial, nutritional, and health-promoting properties of royal jelly (RJ) have been widely confirmed, the effects of storage temperature and time on RJ quality remain to be further explored. Herein, the antimicrobial and proteomic dynamics of RJ stored under different conditions were comprehensively investigated to identify consistent and sensitive markers of RJ degradation. We confirmed the negative correlation between antimicrobial properties and increased the storage temperature and duration in RJ. Using surface plasmon resonance, we showed the protein degradation-induced conformation changes in RJ, which reflected the overall variation in RJ proteins caused by the storage conditions. Further proteomic and western blotting analyses demonstrated the sensitivity and reliability of major RJ protein 4 (MRJP4) as a measure of temperature- and time-dependent RJ changes. Based on these results, we developed a colloidal gold immunoassay strip for MRJP4 detection, providing a reliable, simple, and rapid method for the evaluation of RJ freshness.
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http://dx.doi.org/10.1021/acs.jafc.1c02843DOI Listing
September 2021

Investigation of morphological changes of HPS membrane caused by cecropin B through scanning electron microscopy and atomic force microscopy.

J Vet Sci 2021 Sep 7;22(5):e59. Epub 2021 Jul 7.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.

Background: Antimicrobial peptides (AMPs) have been identified as promising compounds for consideration as novel antimicrobial agents.

Objectives: This study analyzed the efficacy of cecropin B against isolates through scanning electron microscopy (SEM) and atomic force microscopy (AFM) experiments.

Results: Cecropin B exhibited broad inhibition activity against 15 standard (HPS) strains and 5 of the clinical isolates had minimum inhibition concentrations (MICs) ranging from 2 to 16 μg/mL. Microelectrophoresis and hexadecane adsorption assays indicated that the more hydrophobic and the higher the isoelectric point (IEP) of the strain, the more sensitive it was to cecropin B. Through SEM, multiple blisters of various shapes and dents on the cell surface were observed. Protrusions and leakage were detected by AFM.

Conclusions: Based on the results, cecropin B could inhibit HPS via a pore-forming mechanism by interacting with the cytoplasmic membrane of bacteria. Moreover, as cecropin B concentration increased, the bacteria membrane was more seriously damaged. Thus, cecropin B could be developed as an effective anti-HPS agent for use in clinical applications.
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http://dx.doi.org/10.4142/jvs.2021.22.e59DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460462PMC
September 2021

[Stability of Soil Aggregates at Different Altitudes in Qinling Mountains and Its Coupling Relationship with Soil Enzyme Activities].

Huan Jing Ke Xue 2021 Sep;42(9):4510-4519

Shaanxi Key Laboratory of Earth Surface System and Environmental Carrying Capacity, College of Urban and Environmental Sciences, Northwest University, Xi'an 710127, China.

To explore changes in soil aggregate stability along an elevation gradient, and its regulating factors, soil samples were taken from the 0-10 cm surface layer at 3 different elevations on Taibai Mountain. We measured and analyzed the distribution of soil aggregates, physical and chemical properties, microbial biomass, and extracellular enzymes. The results showed that: ① the soil aggregates from the 3 elevations had mean weight diameters (MWD) of 2.17 mm, 1.83 mm, and 1.82 mm (increasing elevation), and geometric mean diameters (GMD) of 1.66 mm, 1.39 mm, and 1.32 mm, respectively. ② The change in soil aggregate stability along an elevation gradient was regulated by extracellular enzymes in the soil, in particular, the LAP in soil meso-aggregate and the BG in soil micro-aggregate. ③ Microorganisms can alleviate the N limitation at high elevations by adjusting the relative production of extracellular enzymes and altering nutrient utilization efficiency, which also changes soil aggregate stability along an elevation gradient. The results of this study have important scientific significance for soil quality evaluation and ecological environment protection in Taibai Mountain.
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http://dx.doi.org/10.13227/j.hjkx.202101236DOI Listing
September 2021

Unraveling the Synergy of Chemical Hydroxylation and the Physical Heterointerface upon Improving the Hydrogen Evolution Kinetics.

ACS Nano 2021 Sep 18;15(9):15017-15026. Epub 2021 Aug 18.

Center for Integrated Nanostructure Physics, Institute for Basic Science (IBS), Sungkyunkwan University, Suwon 16419, Republic of Korea.

Efficient transition metal oxide electrocatalysts for the alkaline hydrogen evolution reaction (HER) have received intensive attention to energy conversion but are limited by their sluggish water dissociation and unfavorable hydrogen migration and coupling. Herein, systematic density functional theory (DFT) predicts that on representative NiO, the hydroxylation (OH) and heterointerface coupled with metallic Cu can respectively reduce the energy barrier of water dissociation and facilitate hydrogen spillover. Motivated by theoretical predictions, we subtly designed a delicate strategy to realize the electrochemical OH modification in KOH with moderate concentration (HO-NiO) and to channel rapid hydrogen spillover at the heterointerface of HO-NiO and Cu, ensuring an enhanced HER kinetic. This HO-NiO/Cu is systematically investigated by XAS and electrochemical simulations, verifying its extraordinary merits for HER including the enhanced water dissociation, alleviated oxophilicity that is advantageous for consecutive adsorptions of water, and accelerated hydrogen spillover, thereby exhibiting superb HER activity with 33 and 310 mV overpotentials at the current densities of 10 and 1000 mA cm in 1.0 M KOH, outperforming the Pt/C. This study might provide a reasonable strategy for the functionalized design of superior electrocatalysts.
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http://dx.doi.org/10.1021/acsnano.1c05324DOI Listing
September 2021

Diabetes is associated with poor short-term prognosis in patients with hepatitis B virus-related acute-on-chronic liver failure.

Hepatol Int 2021 Oct 10;15(5):1093-1102. Epub 2021 Aug 10.

Department of Infectious Diseases, Affiliated Hospital of Zunyi Medical University, 201 Dalian Street, Zunyi, 563003, Guizhou, China.

Background/purpose: Associations between the disturbances in glucose homeostasis and prognosis in patients with hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) remain unclear. This study was conducted to investigate the clinical characteristics of disturbances in glucose homeostasis and their associations with 90-day mortality in patients with HBV-related ACLF.

Methods: Ninety-six patients with HBV-related ACLF without pre-existing diabetes were prospectively included. Glucose abnormalities were diagnosed based on fasting plasma glucose and oral glucose tolerance test results on admission and during follow-up. Homeostasis model assessment was used to establish insulin resistance (HOMA2-IR), insulin sensitivity (HOMA2-IS) and HOMA2-β-cell function (HOMA2-β). Multivariate Cox proportional hazards analysis was used to identify independent risk factors for death within 90 days after admission.

Results: Among 96 patients with ACLF, 51 (53.1%) had diabetes, 29 (30.2%) had impaired glucose tolerance (IGT), and 17 (17.7%) had hypoglycemia. Patients with diabetes had significantly lower levels of HOMA2-β than did patients with normal glucose tolerance. Of 22 patients with diabetes or IGT and without anti-hyperglycemic treatment, 8 (36.4%) exhibited regression of their glucose metabolism disorders after a follow-up of 32.8 ± 28.8 days, and higher platelet levels were associated with regression. Twenty-five patients (25.0%) with ACLF died of liver failure within 90 days. Diabetes [odds ratio (OR) 3.601, 95% confidence interval (CI) 1.342-9.661] and age (OR 1.045, 95% CI 1.010-1.082) were the independent risk factors associated with mortality.

Conclusion: Impaired pancreatic β-cell function is related to diabetes development, and diabetes is associated with high mortality in patients with chronic HBV-related ACLF.
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http://dx.doi.org/10.1007/s12072-021-10243-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352756PMC
October 2021

Reacquainting the Electrochemical Conversion Mechanism of FeS Sodium-Ion Batteries by Operando Magnetometry.

J Am Chem Soc 2021 Aug 9;143(32):12800-12808. Epub 2021 Aug 9.

College of Physics, University-Industry Joint Center for Ocean Observation and Broadband Communication, State Key Laboratory of Bio-Fibers and Eco-Textiles Qingdao University, Qingdao 266071, China.

In spite of the excellent electrochemical performance in lithium-ion batteries (LIBs), transition-metal compounds usually show inferior capacity and cyclability in sodium-ion batteries (SIBs), implying different reaction schemes between these two types of systems. Herein, coupling operando magnetometry with electrochemical measurement, we peformed a comprehensive investigation on the intrinsic relationship between the ion-embedding mechanisms and the electrochemical properties of the typical FeS/Na (Li) cells. Operando magnetometry together with ex-situ transmission electron microscopy (TEM) measurement reveal that only part of FeS is involved in the conversion reaction process, while the unreactive parts form "inactive cores" that lead to the low capacity. Through quantification with Langevin fitting, we further show that the size of the iron grains produced by the conversion reaction are much smaller in SIBs than that in LIBs, which may lead to more serious pulverization, thereby resulting in worse cycle performance. The underlying reason for the above two above phenomena in SIBs is the sluggish kinetics caused by the larger Na-ion radius. Our work paves a new way for the investigation of novel SIB materials with high capacity and long durability.
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http://dx.doi.org/10.1021/jacs.1c06115DOI Listing
August 2021

Evaluation of Cloud 3D Printing Order Task Execution Based on the AHP-TOPSIS Optimal Set Algorithm and the Baldwin Effect.

Micromachines (Basel) 2021 Jul 6;12(7). Epub 2021 Jul 6.

Shandong Longli Electronic Co., Ltd., Linyi 276000, China.

Focusing on service control factors, rapid changes in manufacturing environments, the difficulty of resource allocation evaluation, resource optimization for 3D printing services (3DPSs) in cloud manufacturing environments, and so on, an indicator evaluation framework is proposed for the cloud 3D printing (C3DP) order task execution process based on a Pareto optimal set algorithm that is optimized and evaluated for remotely distributed 3D printing equipment resources. Combined with the multi-objective method of data normalization, an optimization model for C3DP order execution based on the Pareto optimal set algorithm is constructed with these agents' dynamic autonomy and distributed processing. This model can perform functions such as automatic matching and optimization of candidate services, and it is dynamic and reliable in the C3DP order task execution process based on the Pareto optimal set algorithm. Finally, a case study is designed to test the applicability and effectiveness of the C3DP order task execution process based on the analytic hierarchy process and technique for order of preference by similarity to ideal solution (AHP-TOPSIS) optimal set algorithm and the Baldwin effect.
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http://dx.doi.org/10.3390/mi12070801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305594PMC
July 2021

Home blood pressure telemonitoring for improving blood pressure control in middle-aged and elderly patients with hypertension.

J Clin Hypertens (Greenwich) 2021 09 5;23(9):1744-1751. Epub 2021 Aug 5.

Research Institute of Hypertension, Department of Cardiovascular Medicine, The Second Affiliated Hospital of Baotou Medical College, Baotou, Inner Mongolia, P.R. China.

The blood pressure (BP) control rate among treated hypertensives in China remains low at 37.5%. The relationship between home blood pressure telemonitoring (HBPT) and BP control is controversial. The authors aimed to investigate the relationship between HBPT and BP control in middle-aged and elderly hypertensives. In total, 252 hypertension patients aged between 60 and 79 years were enrolled. The patients were given either HBPT through interactive platforms between physicians and patients (telemonitoring group, n = 126) or conventional management (routine management group, n = 126). All patients were followed-up for 15 months. BP control was defined as home systolic blood pressure < 135 mm Hg and home diastolic blood pressure < 85 mm Hg. At baseline, there were no significant differences in the baseline BP control rate (p = .083). However, after 15 months, the BP control rate improved in both groups, and the telemonitoring group (71.3%) had a significantly higher BP control than the routine management group (49.8%) (p < .001). The change of BP control rate from baseline in the routine management group increased by 26.1%, and that of the telemonitoring group increased by 35.4%. The results of the fully adjusted binary logistic regression showed that HBPT was positively associated with BP control after adjusting for confounders (OR = 4.15, 95% CI 2.05-8.39). Similar results were observed after 3, 9, and 12 months. The association of HBPT with BP control was similar in subgroups. In conclusions, HBPT is recommended for BP control in middle-aged and elderly hypertensives in the community setting.
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http://dx.doi.org/10.1111/jch.14341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678721PMC
September 2021

All-Climate Aluminum-Ion Batteries Based on Binder-Free MOF-Derived [email protected]/CNT Cathode.

Nanomicro Lett 2021 Jul 23;13(1):159. Epub 2021 Jul 23.

Nanomaterials Centre, School of Chemical Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland, 4072, Australia.

Aluminum-ion batteries (AIBs) are promising next-generation batteries systems because of their features of low cost and abundant aluminum resource. However, the inferior rate capacity and poor all-climate performance, especially the decayed capacity under low temperature, are still critical challenges toward high-specific-capacity AIBs. Herein, we report a binder-free and freestanding metal-organic framework-derived [email protected]/carbon nanotube ([email protected]/CNT) as a novel all-climate cathode in AIBs working under a wide temperature window between -25 and 50 °C with exceptional flexibility. The resultant cathode not only drastically suppresses the side reaction and volumetric expansion with high capacity and long-term stability but also greatly enhances the kinetic process in AIBs with remarkable rate capacity (above 151 mAh g at 2 A g) at room temperature. More importantly, to break the bottleneck of the inherently low capacity in graphitic material-based all-climate AIBs, the new hierarchical conductive composite [email protected]/CNT highly promotes the all-climate performance and delivers as high as 117 mAh g capacity even under -25 °C. The well-designed metal sulfide electrode with remarkable performance paves a new way toward all-climate and flexible AIBs.
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http://dx.doi.org/10.1007/s40820-021-00682-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302704PMC
July 2021

Efficacy and safety of pulsatile gonadotropin-releasing hormone therapy in patients with congenital hypogonadotropic hypogonadism: a multicentre clinical study.

Ann Transl Med 2021 Jun;9(12):962

NHC Key Laboratory of Endocrinology, Peking Union Medical College Hospital; Department of Endocrinology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Background: Pulsatile gonadotropin-releasing hormone (GnRH) therapy may restore function of the hypothalamus-pituitary-gonad axis and induce spermatogenesis in male patients with congenital hypogonadotropic hypogonadism (CHH). The study sought to test the reliability of a newly developed Innopump hormone pump, and to confirm the efficacy and safety of pulsatile GnRH therapy (by Innopump hormone pump) in CHH patients.

Methods: From November 2017 to November 2018, 28 male patients with CHH were treated with pulsatile GnRH at Peking Union Medical College Hospital, Beijing Chaoyang Hospital, and Shandong Provincial Hospital. A prospective, self-controlled, 7-day clinical trial was conducted. The primary outcome measures were the efficacy and safety of pulsatile GnRH therapy (which was administered via the Innopump hormone pump). The secondary outcome measures included total serum testosterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels.

Results: All of the patients participated the clinical study. For 7 days, a dosage prescribed by doctors was accurately administered by the Innopump hormone pump, and recorded by the pump. During the treatment, LH and FSH levels gradually increased to 2.66±1.74 and 5.05±3.03 IU/L, respectively. Upper respiratory tract infection in 1 patient and slight nausea in another patient were reported, which were confirmed to be unrelated to the pulsatile GnRH therapy.

Conclusions: The Innopump hormone pump was found to be reliable in drug administration, and to have an accurate alarming system. It effectively and safely treated patients with CHH. Pulsatile GnRH therapy may produce a physiological pattern of GnRH secretion, and re-establish pituitary-gonad axis function by increasing gonadotropin levels.
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http://dx.doi.org/10.21037/atm-21-1632DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267282PMC
June 2021

A temperature-dependent phosphorus doping on TiCT MXene for enhanced supercapacitance.

J Colloid Interface Sci 2021 Dec 12;604:239-247. Epub 2021 Jul 12.

School of Chemical Engineering, China University of Petroleum (East), Qingdao 266580, China. Electronic address:

A novel type of phosphorus doped TiCT MXene nanosheets (P-TiCT) is synthesized via a facile and controllable strategy of annealing MXene nanosheets with the presence of sodium hypophosphite. A combination of theoretical density functional theory calculation and experimental X-ray photoelectron spectroscopy discloses that the doped P atoms are prone to fill into Ti vacancies first due to their lowest formation free energy (ΔG = -0.028 eV·Å) and next to bond with surface terminals on MXene layers (ΔG = 0.013 eV·Å), forming P-C and P-O species, respectively. More importantly, the as-obtained P-TiCT is, for the first time, investigated as the electrode material for supercapacitors, demonstrating a significantly boosted electrochemical performance by P doping. As a result, P-TiCT electrode delivers a high specific capacitance of 320 F·g at a current density of 0.5 A·g (much higher than 131 F·g for undoped MXene), an ultrahigh rate retention of 83.8% capacitance at 30 A·g, and a high cycling stability over continuous 5000 cycles.
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http://dx.doi.org/10.1016/j.jcis.2021.06.020DOI Listing
December 2021

Chimeric Peptides Quickly Modify the Surface of Personalized 3D Printing Titanium Implants to Promote Osseointegration.

ACS Appl Mater Interfaces 2021 Jul 14;13(29):33981-33994. Epub 2021 Jul 14.

School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, People's Republic of China.

Titanium (Ti) and titanium alloys have been widely used in the field of biomedicine. However, the unmatched biomechanics and poor bioactivities of conventional Ti implants usually lead to insufficient osseointegration. To tackle these challenges, it is critical to develop a novel Ti implant that meets the bioadaptive requirements for load-bearing critical bone defects. Notably, three-dimensional (3D)-printed Ti implants mimic the microstructure and mechanical properties of natural bones. Additionally, eco-friendly techniques based on inorganic-binding peptides have been applied to modify Ti surfaces. Herein, in our study, Ti surfaces were modified to reinforce osseointegration using chimeric peptides constructed by connecting W9, RP1P, and minTBP-1 directly or (GP), respectively. PR1P is derived from the extracellular VEGF-binding domain of prominin-1, which increases the expression of VEGF and promotes the binding of VEGF to endothelial cells, thereby accelerating angiogenesis. W9 induces osteoblast differentiation in bone marrow mesenchymal stem cells and human mesenchymal stem cells to promote bone formation. Overall, chimeric peptides promote osseointegration by promoting angiogenesis and osteogenesis. Additionally, chimeric peptides with P3&4 were more effective than those with P1&2 in improving osseointegration, which might be ascribed to the capacity of P3&4 to provide a greater range for chimeric peptides to express their activity. This work successfully used chimeric peptides to modify 3D-Ti implant surfaces to improve osseointegration on the implant-bone surface.
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http://dx.doi.org/10.1021/acsami.1c11207DOI Listing
July 2021

Serum NSE and S100B protein levels for evaluating the impaired consciousness in patients with acute carbon monoxide poisoning.

Medicine (Baltimore) 2021 Jun;100(25):e26458

Department of Emergency.

Abstract: The aim of this study was to investigate the associations between the levels of neuron-specific enolase (NSE) and S100B protein and coma duration, and evaluate the optimal cut-off values for prediction coma duration ≥ 72 hours in patients with acute carbon monoxide poisoning (ACOP).A total of 60 patients with ACOP were divided into 3 following groups according to their status of consciousness and coma duration at admission: Awake group [Glasgow Coma Scale score (GCS score) ≥ 13 points], Coma < 72 hours group (GCS score < 13 points and coma duration < 72 h), and Coma ≥ 72 hours group (GCS score < 13 points and coma duration ≥ 72 h). The levels of serum NSE and S100B protein were measured after admission.There were significant differences in GCS score, carbon monoxide (CO) exposure time, NSE, and S100B levels between the Coma ≥ 72 h group and the Awake group, and between the Coma < 72 h group and the Awake group. Significant differences in GCS score, NSE, and S100B levels were also found between Coma ≥ 72 h group and Coma < 72 h group. Correlation analysis showed that NSE and S100B were positively correlated (rs = 0.590, P < .01); NSE and S100B were negatively correlated with GCS score (rs = -0.583, rs = -0.590, respectively, both P < .01). The areas under the curve (AUCs) of NSE, S100B, and GCS score to predict the coma duration ≥ 72 hours were 0.754, 0.791, and 0.785, respectively. Pairwise comparisons did not show differences among the 3 groups (all P > .05). The sensitivity and specificity of NSE prediction with a cut-off value of 13 μg/L were 80% and 64%, respectively, and those of S100B prediction with a cut-off value of 0.43 μg/L were 70% and 88%, respectively.The NSE and S100B protein levels were significantly correlated with the degree of impaired consciousness and had the same clinical value in predicting coma duration of ≥ 72 hours in patients with ACOP.
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http://dx.doi.org/10.1097/MD.0000000000026458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238324PMC
June 2021

First Report of Anthracnose on Hymenocallis littoralis Caused by Colletotrichum siamense in China.

Plant Dis 2021 Jun 4. Epub 2021 Jun 4.

Department of Biotechnology, College of Coastal Agricultural Sciences of Guangdong Ocean University, Zhanjiang, China;

Spider lily (Hymenocallis littoralis (Jacq.) Salisb.) is a widely cultivated horticultural plant worldwide and has ornamental and medicinal value. Spider lily plants were seriously affected by a leaf spot disease in the campus of Guangdong Ocean University and gardens in Zhanjiang city in February 2018 with an incidence of 30 to 100%. Affected leaves usually developed small circular purple spots, which enlarged to oval spots and large irregular spots. The spots were brown at the center, deep purple at the border and surrounded by a yellow halo. Diseased cultivars were collected in Zhanjiang city, Gangzhou city in Guangdong province and and Zhangping city in Fujian province. Symptomatic leaf samples were disinfested with 1% NaOCl, and cultured on sucrose agar (PSA) at 28 °C for one week. Ten single-spore isolates were recovered from PSA medium. Colonies developing on PSA were grayish white with a regular border. Conidia were straight, hyaline with rounded ends, 4.3 to 6.1×12.8 to 32.1μm (n = 50 conidia of each isolate). Fungal mycelia were hyaline, septate, and branched. Conidia were born on a long conidiogenous cell, appressoria were oval, 6.7 to 10.7 × 5.2 to 6.2 μm (n=50). The isolates were morphologically identified as Colletotrichum sp. (Weir et al. 2012). Tests of pathogenicity were performed according to Koch's postulates using three isolates. Fresh wounds were made with a sterile needle on the healthy surface of leaves of H. littoralis at the 4- to 6-leaf stage and each leaf was covered with a piece of cotton drenched with 200 μL of conidial suspension (106 conidia/ml) from each isolate. Control seedlings were inoculated identically except sterile water was used to drench the cotton. Inoculated plants were placed in a moisturizing light incubator at 25℃ and 80% humidity under a 12-h light/dark cycle for 20 days and examined daily to monitor disease symptom development. Small round brown spots were observed at the inoculation sites 3 days after the inoculation. The brown spots developed into large brown lesions 5 days after inoculation. There were no symptoms observed in the water-inoculated plants. A Colletotrichum spp. strain based on morphology was consistently reisolated from leaves lesions fulfilling Koch's postulates. For molecular identification, the internal transcribed spacer (ITS) region of ribosomal DNA, calmodulin (CAL), Tublin (Tub) and Apmat loci of three isolates were amplified using primer pairs of ITS4/ITS5, CL1C/CL2C, T1/T2 and AM-F/AM-R (Sharma et al. 2015). A phylogenetic tree derived from a neighbor-joining analysis of a concatenated alignment of ITS, CAL, Tub and ApMAT sequences was created. The accession numbers of three isolates GZHLCG, ZJHLCG and FJHLCG used in this study were MW553083, MN540457, MN540458 for ITS, MW553087- MW553089 for CL, MW553090-MW553092 for Tub and MW553084-MW553086 for ApMAT. The sequences of the three isolates were aligned with related species of Colletotrichum (Sharma et al. 2015). Analyses based on concatenated data sets of four genes showed that the sequences had high levels of identity to those of the C. siamense strains. According to both morphological and sequence analyses, the H. littoralis pathogen was identified as C. siamense. There is a report of foliar diseases on H. littoralis caused by Colletotrichum sp. (Tan et al., 2009). To our knowledge, this is the first report of anthracnose on H. littoralis caused by C. siamense in China. Identification of the pathogen provide valuable information for diagnosis and controlling this disease in H. littoralis.
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http://dx.doi.org/10.1094/PDIS-02-21-0405-PDNDOI Listing
June 2021
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