Publications by authors named "Han Hao"

173 Publications

Dissecting the Flash Chemistry of Electrogenerated Reactive Intermediates by Microdroplet Fusion Mass Spectrometry.

Angew Chem Int Ed Engl 2021 Jun 15. Epub 2021 Jun 15.

Najing University, Chemistry, Xianlin Ave. 163, , 210023, Nanjing, CHINA.

A novel mass spectrometric method for probing the flash chemistry of electrogenerated reactive intermediates was developed based on rapid collision mixing of electrosprayed microdroplets by using a theta-glass capillary. The two individual microchannels of the theta-glass capillary are asymmetrically or symmetrically fabricated with a carbon bipolar electrode to produce intermediates in situ . Microdroplets containing the newly formed intermediates collide with those of the invoked reactants at sub-10 microsecond level, making it a powerful tool for exploring their ultrafast initial transformations. As a proof-of-concept, we present the identification of the key radical cation intermediate in the oxidative dimerization of 8-methyl-1,2,3,4-tetrahydroquinoline and also the first revealment of previously hidden nitrenium ion involved reaction pathway in the C-H/N-H cross-coupling between N,N'-dimethylaniline and phenothiazine.
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http://dx.doi.org/10.1002/anie.202106945DOI Listing
June 2021

Hybrid AI-assistive diagnostic model permits rapid TBS classification of cervical liquid-based thin-layer cell smears.

Nat Commun 2021 06 10;12(1):3541. Epub 2021 Jun 10.

Department of Pathology, Guangdong Provincial Women's and Children's Dispensary, Shenzhen, Guangdong Province, PR China.

Technical advancements significantly improve earlier diagnosis of cervical cancer, but accurate diagnosis is still difficult due to various factors. We develop an artificial intelligence assistive diagnostic solution, AIATBS, to improve cervical liquid-based thin-layer cell smear diagnosis according to clinical TBS criteria. We train AIATBS with >81,000 retrospective samples. It integrates YOLOv3 for target detection, Xception and Patch-based models to boost target classification, and U-net for nucleus segmentation. We integrate XGBoost and a logical decision tree with these models to optimize the parameters given by the learning process, and we develop a complete cervical liquid-based cytology smear TBS diagnostic system which also includes a quality control solution. We validate the optimized system with >34,000 multicenter prospective samples and achieve better sensitivity compared to senior cytologists, yet retain high specificity while achieving a speed of <180s/slide. Our system is adaptive to sample preparation using different standards, staining protocols and scanners.
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http://dx.doi.org/10.1038/s41467-021-23913-3DOI Listing
June 2021

Surgical treatment of sternum comminuted fracture with memory alloy embracing fixator.

J Thorac Dis 2021 Apr;13(4):2194-2202

Department of Thoracic Surgery, The 901st Hospital of the Joint Logistics Support Force of PLA, Hefei, China.

Background: Sternal fracture can result from multiple types of severe chest trauma and carries significant risk. Surgical fixation is an effective method for sternal fracture.

Methods: The clinical data of patients with sternal fractures who presented to our hospital between August 2016 and July 2019 were collected. The 42 patients were divided into three groups, with 15 patients treated by internal fixation with NI-TI memory alloy embracing fixator, 10 patients receiving steel wire fixation, and 17 who received non-surgical treatment and who was designated as a control (conservative) group. Differences in clinical indices included the duration of surgery, blood loss, hospitalization, wound healing, hospitalization expenses, VAS scores, and patient satisfaction scores between the three groups was compared. The analysis of variance and t-test were used for quantitative variables, which were approximately normally distributed. Dichotomous data were compared used Pearson χ or Fisher's exact test, and a P value less than 0.05 was considered as statistically significant.

Results: All patients were cured, and there were no significant differences in general clinical features between the three groups (P>0.05). Thoracic deformity in the surgical groups was corrected anatomically and received better pain scores, while patients in the NI-TI memory alloy embracing fixator group showed advantages of bleeding and patient satisfaction (P<0.05).

Conclusions: Operative treatment for a sternal fracture is safe, effective and can quickly restore the stability of the thorax. Memory alloy embracing fixator is markedly superior to other fixator materials.
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http://dx.doi.org/10.21037/jtd-20-3603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107563PMC
April 2021

Combination of Urine Exosomal mRNAs and lncRNAs as Novel Diagnostic Biomarkers for Bladder Cancer.

Front Oncol 2021 27;11:667212. Epub 2021 Apr 27.

Department of Clinical Laboratory, Peking University First Hospital, Beijing, China.

Background: The recent discovery of miRNAs and lncRNAs in urine exosomes has emerged as promising diagnostic biomarkers for bladder cancer (BCa). However, mRNAs as the direct products of transcription has not been well evaluated in exosomes as biomarkers for BCa diagnosis. The purpose of this study was to identify tumor progression-related mRNAs and lncRNAs in urine exosomes that could be used for detection of BCa.

Methods: RNA-sequencing was performed to identify tumor progression-related biomarkers in three matched superficial tumor and deep infiltrating tumor regions of muscle-invasive bladder cancer (MIBC) specimens, differently expressed mRNAs and lncRNAs were validated in TCGA dataset (n = 391) in the discovery stage. Then candidate RNAs were chosen for evaluation in urine exosomes of a training cohort (10 BCa and 10 healthy controls) and a validation cohort (80 BCa and 80 healthy controls) using RT-qPCR. The diagnostic potential of the candidates were evaluated by receiver operating characteristic (ROC) curves.

Results: RNA sequencing revealed 8 mRNAs and 32 lncRNAs that were significantly upregulated in deep infiltrating tumor region. After validation in TCGA database, 10 markedly dysregulated RNAs were selected for further investigation in urine exosomes, of which five (mRNAs: KLHDC7B, CASP14, and PRSS1; lncRNAs: MIR205HG and GAS5) were verified to be significantly dysregulated. The combination of the five RNAs had the highest AUC to disguising the BCa (0.924, 95% CI, 0.875-0.974) or early stage BCa patients (0.910, 95% CI, 0.850 to 0.971) from HCs. The expression levels of these five RNAs were correlated with tumor stage, grade, and hematuria degrees.

Conclusions: These findings highlight the potential of urine exosomal mRNAs and lncRNAs profiling in the early diagnosis and provide new insights into the molecular mechanisms involved in BCa.
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http://dx.doi.org/10.3389/fonc.2021.667212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111292PMC
April 2021

3D reconstruction of the specular surface using an iterative stereoscopic deflectometry method.

Opt Express 2021 Apr;29(9):12867-12879

Phase measuring deflectometry (PMD) is an effective technique for three-dimensional measurement of specular surfaces. However, the ambiguity of monoscopic PMD and the time-consuming searching process of stereoscopic PMD are challenges for specular surface reconstruction. To solve it, we propose an iterative reconstruction algorithm for the stereoscopic phase measuring deflectometry system free of the time-consuming searching process for each pixel. An arbitrary seed point on the specular surface is accurately obtained via a coarse-to-fine optimization means without any other expensive and complicate auxiliaries. Then, a plane with the height of seed point is set as the initial surface form for the iteration, in which the pinhole model is used to find the linear relation to update the surface form. The converging height is the output as the final result. Simulations and experiments verify the feasibility and efficiency of our proposed method based on the stereoscopic phase measuring deflectometry system. The accuracy and robustness are comprehensively evaluated as well.
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http://dx.doi.org/10.1364/OE.421898DOI Listing
April 2021

A 10.8 µW Neural Signal Recorder and Processor With Unsupervised Analog Classifier for Spike Sorting.

IEEE Trans Biomed Circuits Syst 2021 Apr 25;15(2):351-364. Epub 2021 May 25.

Implantable brain machine interfaces for treatment of neurological disorders require on-chip, real-time signal processing of action potentials (spikes). In this work, we present the first spike sorting SoC with integrated neural recording front-end and analog unsupervised classifier. The event-driven, low power spike sorter features a novel hardware-optimized, K-means based algorithm that effectively eliminates duplicate clusters and is implemented using a novel clockless and ADC-less analog architecture. The 1.4 mm chip is fabricated in a 180-nm CMOS SOI process. The analog front-end achieves a 3.3 μV noise floor over the spike bandwidth (400 - 5000 Hz) and consumes 6.42 μW from a 1.5 V supply. The analog spike sorter consumes 4.35 μW and achieves 93.2% classification accuracy on a widely used synthetic test dataset. In addition, higher than 93% agreement between the chip classification result and that of a standard spike sorting software is observed using pre-recorded real neural signals. Simulations of the implemented spike sorter show robust performance under process-voltage-temperature variations.
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http://dx.doi.org/10.1109/TBCAS.2021.3076147DOI Listing
April 2021

Indocyanine green fluorescence imaging for laparoscopic complex upper urinary tract reconstructions: a comparative study.

Transl Androl Urol 2021 Mar;10(3):1071-1079

Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, National Urological Cancer Centre, Beijing, China.

Background: To describe our technique for using an intraureteral injection of indocyanine green (ICG) and visualization under near-infrared fluorescence (NIRF) to facilitate challenging upper urinary tract reconstructions (UUTRs) and to present the comparative outcomes.

Methods: We collected 36 patients who underwent laparoscopic UUTRs between April 2019 and March 2020, and we divided the patients into two groups based on the use of ICG (ICG group and non-ICG group). Demographic characteristics, perioperative outcomes, and functional outcomes were compared between the two groups.

Results: There were 18 cases in the ICG group and 18 cases in the non-ICG group, respectively. There were no differences in the baseline characteristics between the two groups. The intraoperative time to identification of the ureter (TIU; 20.9±11.7 30.0±14.6 min, P=0.03) and length of postoperative hospital stay (LPHS; 11.1±3.0 16.6±10.0 days, P=0.03) were significantly shorter in the ICG group. There was also a trend for lesser time for locating the stricture (43.0±27.9 55.4±18.6 min, P=0.14) and lower estimated blood loss (EBL) in the ICG group patients (88.3±75.4 91.7±46.2 mL, P=0.22). During the mean 3.8-month follow-up for the ICG group and the 6.2-month for the non-ICG group, there was a trend for more severe complications in the non-ICG group.

Conclusions: Visualizing intraureteral ICG under NIRF is useful in challenging UUTRs, allows for rapid ureteral identification and accurate real-time delineation of the ureteral stricture margins, and provides encouraging follow-up outcomes compared with those in the non-ICG group.
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http://dx.doi.org/10.21037/tau-20-1261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039610PMC
March 2021

Impacts of the New Worldwide Light-Duty Test Procedure on Technology Effectiveness and China's Passenger Vehicle Fuel Consumption Regulations.

Int J Environ Res Public Health 2021 03 19;18(6). Epub 2021 Mar 19.

State Key Laboratory of Automotive Safety and Energy, Tsinghua University, Beijing 100084, China.

As a main measure to promote the development of China's energy-saving and new energy vehicles, the Phase V fuel consumption regulation is dramatically different from the past four phases, especially in the test procedure, moving from the New European Driving Cycle (NEDC) to the worldwide harmonized light duty test cycle (WLTC) and corresponding test procedure (WLTP). The switch of test procedure will not only affect the effectiveness of technologies but also change the fuel consumption target of the industry. However, few studies have systematically investigated the impacts of the new WLTP on the Chinese market. This study establishes a "technology-vehicle-fleet" bottom-up framework to estimate the impacts of test procedure switching on technology effectiveness and regulation stringency. The results show that due to the WLTP being closer to the real driving condition and more stringent, almost all baseline vehicles in the WLTP have higher fuel consumption than that in the NEDC, and diesel vehicles are slightly more impacted than gasoline vehicles. In addition, the impacts are increased with the strengthening of electrification, where the fuel consumption of plug-in hybrid electric vehicles (PHEVs) and range-extended electric vehicles (REEVs) in the WLTP are about 6% higher than that in the NEDC. Engine technologies that gain higher effects in low load conditions, such as turbocharging and downsizing, fuel stratified injection (FSI), lean-burn, and variable valve timing (VVT), are faced with deterioration in the WLTP. Among these, the effect of turbocharging and downsizing shows a maximum decline of 8.5%. The variable compression ratio (VCR) and stoichiometric gasoline direct injection (SGDI) are among the few technologies that benefited from procedure switching, with an average improvement of 1.6% and 0.2% respectively. Except for multi-speed transmissions, which have improvement effects in the WLTP, all automatic transmissions are faced with decreases. From the perspective of the whole fleet and national regulation target, the average fuel consumption in the WLTP will increase by about 7.5% in 2025 compared to 4 L/100 km in the NEDC. According to the current planning of the Chinese government, the fuel consumption target of Phase V is set at 4.6 L/100 km in 2025, which is equivalent to loosening the stringency by 0.3 L/100 km. In Phase VI, the target of 3.2 L/100 km is maintained, which is 30.4% stricter than that of Phase V, and the annual compound tightening rate reaches 7.5%. This means that automakers need to launch their product planning as soon as possible and expand the technology bandwidth to comply with the Phase VI fuel consumption regulation, and the government should evaluate the technical feasibility before determining the evaluation methods and targets of the next phase.
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http://dx.doi.org/10.3390/ijerph18063199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003633PMC
March 2021

Etiology and Ureteral Reconstruction Strategy for Iatrogenic Ureteral Injuries: A Retrospective Single-Center Experience.

Urol Int 2021 19;105(5-6):470-476. Epub 2021 Mar 19.

Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.

Objective: To analyze the etiology, characteristics, and ureteral reconstruction strategies of iatrogenic ureteric injuries in a high-volume center.

Methods: Between September 2010 and August 2019, we retrospectively collected patients who underwent ureteral reconstruction due to iatrogenic ureteric injuries. Patient profiles, laboratory data, imaging studies, perioperative data, and complications were recorded.

Results: Sixty-eight patients were enrolled in this study. The upper, middle, and lower thirds of the ureter were affected in 30, 2, and 36 cases, respectively. Of the 68 ureteric injuries, 69.1% occurred during urological procedures, followed by gynecological procedures, general surgery, radiotherapy, and orthopedic surgery. The majority of urological injuries (41, 87.2%) occurred due to stone removal. There was a significant difference in the age, sex, and location of ureteric injuries between the urological and nonurological groups. The median follow-up time was 17.9 months. The overall symptom remission rate was 91.2% and ranged from 87.5 to 100% for different reconstructive surgeries.

Conclusions: Urological procedures were the most common cause of iatrogenic ureteric injury; thus, extra care should be taken. Timely detection and appropriate treatment of the ureteric injuries are necessary. Treatment strategies should be depended on the location and length of injury.
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http://dx.doi.org/10.1159/000511141DOI Listing
March 2021

Laparoscopic versus open radical cystectomy in 607 patients with bladder cancer: Comparative survival analysis.

Int J Urol 2021 Jun 13;28(6):673-680. Epub 2021 Mar 13.

Department of Urology, Peking University First Hospital, Beijing, China.

Objectives: To compare perioperative and oncologic survival outcomes between laparoscopic radical cystectomy and open radical cystectomy.

Methods: A total of 607 patients underwent open radical cystectomy (n = 412) or laparoscopic radical cystectomy (n = 195) at a single academic institution from January 2006 to April 2017. Their medical records were retrospectively analyzed. One-to-one propensity score matching was carried out to reduce selection bias. Estimated blood loss and complications were compared. Overall survival, cancer-specific survival and progression-free survival estimates for all patients and patients with locally advanced bladder cancer were analyzed using the Kaplan-Meier method.

Results: Either before or after matching, the laparoscopic radical cystectomy group had less estimated blood loss (P < 0.001 and P < 0.001) and fewer complications (P < 0.001 and P = 0.008). There was no difference in the overall survival (P = 0.216 and P = 0.961) and progression-free survival (P = 0.826 and P = 0.462) for all the patients having either laparoscopic radical cystectomy or open radical cystectomy. However, the 5-year progression-free survival of open radical cystectomy was higher than that of laparoscopic radical cystectomy (P = 0.019 and P = 0.021) for patients with locally advanced bladder cancer.

Conclusions: Laparoscopic radical cystectomy is superior to open radical cystectomy in terms of perioperative outcomes, and similar to open radical cystectomy in terms of oncologic outcomes for patients with early stage bladder cancer. However, for patients with locally advanced bladder cancer, laparoscopic radical cystectomy seems to be associated with shorter progression-free survival than open radical cystectomy.
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http://dx.doi.org/10.1111/iju.14537DOI Listing
June 2021

Using Catalysts To Make Catalysts: Titanium-Catalyzed Hydroamination To Access -Ligands for Assembling Catalysts in One Pot.

Org Lett 2021 Mar 4;23(6):1974-1979. Epub 2021 Mar 4.

Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC Canada, V6T1Z1.

Using a diamido-bis(amidate) titanium precatalyst, the hydroamination of alkynylphosphines afforded phosphinoenamine products. After reduction, 2-aminophosphines are prepared in excellent yield and on gram scale. A broad variety of alkynylphosphines and primary amines with different electronic and steric features are tolerated in this sequential transformation, enabling the rapid assembly of a collection of ligands. Additionally, intermediate phosphinoenamines can be used directly as proligands for coordination to transition metals using protonolysis or salt metathesis reactions. These transformations result in easy-to-use one pot protocols to prepare metal ,-complexes for catalysis or small molecule activation.
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http://dx.doi.org/10.1021/acs.orglett.0c04212DOI Listing
March 2021

Effects of KAI gene expression on ferroptosis in pancreatic cancer cells.

Mol Med Rep 2021 02 22;23(2). Epub 2020 Dec 22.

Department of Gastroenterology, General Hospital of Northern Theater Command, Shenyang, Liaoning 110840, P.R. China.

The specific role and mechanism of ferroptosis in the development of pancreatic cancer (PC) remain to be elucidated. The present study aimed to investigate the effects of the overexpression of the KAI1 gene on the ferroptosis of the human PC cell line MIA PaCa‑2. MIA PaCa‑2 cells infected with pCMV‑KAI1 and selected by G418 and KAI1 protein were analyzed by western blotting. The MIA PaCa‑2 cells with a stable expression of the KAI1 gene were termed MIA PaCa‑2‑KAI1. The proliferative capacities of MIA PaCa‑2 and MIA PaCa‑2‑KAI1 cells were detected using Cell Counting Kit‑8. The reactive oxygen species (ROS) in the cells were compared by flow cytometry. The expressions of ferroportin (FPN) and glutathione peroxidase 4 (GPX4) protein were analyzed by western blotting. The KAI1 stable expression cell line was confirmed and relabeled as MIA PaCa‑2‑KAI1. No significant differences in the proliferation of MIA PaCa‑2 and MIA PaCa‑2‑KAI1 were identified. Following treatment with a ferroptosis blocker, the increase in the proliferation of MIA PaCa‑2‑KAI1 (from 2.06±0.02 to 2.75±0.02) was more evident compared with MIA PaCa‑2 (from 2.94±0.02 to 2.95±0.02; P<0.05). The ROS in MIA PaCa‑2‑KAI1 was significantly higher compared with MIA PaCa‑2 (P<0.05). FPN and GPX4 protein demonstrated higher expression levels in MIA PaCa‑2‑KAI1 compared with MIA PaCa‑2. Moreover, KAI1 exerted an obvious promotion effect on FPN expression. This study identified that the high expression of the KAI1 gene promoted the occurrence of ferroptosis in PC cells through its extensive effect on FPN and GPX4. KAI1‑induced ferroptosis did not significantly inhibit the proliferation of PC cells.
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http://dx.doi.org/10.3892/mmr.2020.11802DOI Listing
February 2021

Primary synchronous colloid adenocarcinoma and squamous cell carcinoma in the same lung: A rare case report.

Medicine (Baltimore) 2021 Feb;100(6):e24700

Department of Pathology, Hebei General Hospital.

Rationale: Double primary lung cancer (DPLC) is a relatively rare type of lung cancers. According to whether the diagnosis interval between lesions is more than 6 months, it can be divided into synchronous DPLC (sDPLC) and metachronous DPLC (mDPLC). Here, we describe a case of sDPLC in which one of the components is a rare colloid adenocarcinoma (CA).

Patient Concerns: A 69-year-old male was admitted to the hospital due to chest distress and shortness of breath for 1 year, getting worse in the last 15 days.

Diagnosis: Both HE staining and IHC supported the diagnosis of CA in the right lower lobe and moderately differentiated squamous cell carcinoma in the right upper lobe.

Interventions: The patient was treated with 3 cycles of adjuvant chemotherapy with pemetrexed and lobaplatin after the right upper lobectomy, wedge resection of the right lower lobe and lymph node dissection under video-assisted thoracoscope.

Outcomes: Our plan was to follow him up with general physical examination, chest-abdomen CT and serum tumor markers every 6 months for 2 years. The patient was still alive until the last follow-up in November 2020.

Lessons: CA of the lung is a rare primary lung adenocarcinoma. The diagnosis should be based on the patient's clinical characteristics, imaging examination and pathological characteristics, and also need to be differentiated from other mucinous adenocarcinomas. Interestingly, our patient developed not only a CA in the right lower lobe, but also a moderately differentiated squamous cell carcinoma in the right upper lobe.
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http://dx.doi.org/10.1097/MD.0000000000024700DOI Listing
February 2021

Junctophilin-4 facilitates inflammatory signalling at plasma membrane-endoplasmic reticulum junctions in sensory neurons.

J Physiol 2021 04 3;599(7):2103-2123. Epub 2021 Mar 3.

School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, UK.

Key Points: Rat somatosensory neurons express a junctional protein, junctophilin-4 (JPH4) JPH4 is necessary for the formation of store operated Ca entry (SOCE) complex at the junctions between plasma membrane and endoplasmic reticulum in these neurons. Knockdown of JPH4 impairs endoplasmic reticulum Ca store refill and junctional Ca signalling in sensory neurons. In vivo knockdown of JPH4 in the dorsal root ganglion (DRG) sensory neurons significantly attenuated experimentally induced inflammatory pain in rats. Junctional nanodomain Ca signalling maintained by JPH4 is an important contributor to the inflammatory pain mechanisms.

Abstract: Junctions of endoplasmic reticulum and plasma membrane (ER-PM junctions) form signalling nanodomains in eukaryotic cells. ER-PM junctions are present in peripheral sensory neurons and are important for the fidelity of G protein coupled receptor (GPCR) signalling. Yet little is known about the assembly, maintenance and physiological role of these junctions in somatosensory transduction. Using fluorescence imaging, proximity ligation, super-resolution microscopy, in vitro and in vivo gene knockdown we demonstrate that a member of the junctophilin protein family, junctophilin-4 (JPH4), is necessary for the formation of store operated Ca entry (SOCE) complex at the ER-PM junctions in rat somatosensory neurons. Thus we show that JPH4 localises to the ER-PM junctional areas and co-clusters with SOCE proteins STIM1 and Orai1 upon ER Ca store depletion. Knockdown of JPH4 impairs SOCE and ER Ca store refill in sensory neurons. Furthermore, we demonstrate a key role of the JPH4 and junctional nanodomain Ca signalling in the pain-like response induced by the inflammatory mediator bradykinin. Indeed, an in vivo knockdown of JPH4 in the dorsal root ganglion (DRG) sensory neurons significantly shortened the duration of nocifensive behaviour induced by hindpaw injection of bradykinin in rats. Since the ER supplies Ca for the excitatory action of multiple inflammatory mediators, we suggest that junctional nanodomain Ca signalling maintained by JPH4 is an important contributor to the inflammatory pain mechanisms.
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http://dx.doi.org/10.1113/JP281331DOI Listing
April 2021

Modified Laparoscopic and Robotic Flap Pyeloplasty for Recurrent Ureteropelvic Junction Obstruction with a Long Proximal Ureteral Stricture: The "Wishbone" Anastomosis and the "Ureteral Plate" Technique.

Urol Int 2021 Feb 10:1-8. Epub 2021 Feb 10.

Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.

Objectives: The aim of the study was to present our modified flap pyeloplasty techniques for recurrent ureteropelvic junction obstruction (UPJO) with a long proximal ureteral stricture and compare outcomes of laparoscopic and robotic procedures.

Materials And Methods: Between March 2018 and January 2020, 21 patients underwent modified laparoscopic or robotic flap pyeloplasty for recurrent UPJO with a long proximal ureteral stricture. Our surgical modifications included the "wishbone" anastomosis and "ureteral plate" technique. Demographic, perioperative, and follow-up data were recorded and compared retrospectively between the groups. Success was defined as subjective pain alleviation and hydronephrosis improvement.

Results: Thirteen modified laparoscopic flap pyeloplasty (mLFP) and 8 modified robotic flap pyeloplasty (mRFP) were performed successfully without conversion. mRFP tended to have shorter overall operative time (142.4 vs. 179.1 min, p = 0.122) and anastomosis time (43.1 vs. 61.0 min, p = 0.093) than mLFP. No difference was found in estimated blood loss (p = 0.723) and pararenal draining time (p = 0.175) between the groups. The mean postoperative hospital stay of mRFP was significantly shorter than that of mLFP (5.0 vs. 8.2 days, p = 0.015). No major complications occurred. During the mean follow-up of 17.9 months, the overall success rate was 90.5%, and there was no significant difference between 2 groups.

Conclusions: The modified flap pyeloplasty could be considered a practical and effective treatment option with a high success rate for recurrent UPJO with a long proximal ureteral stricture, and the robotic procedures showed advantages of higher efficiency and faster recovery.
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http://dx.doi.org/10.1159/000512994DOI Listing
February 2021

Plant sterol ester of α-linolenic acid ameliorates high-fat diet-induced nonalcoholic fatty liver disease in mice: association with regulating mitochondrial dysfunction and oxidative stress via activating AMPK signaling.

Food Funct 2021 Mar 10;12(5):2171-2188. Epub 2021 Feb 10.

Department of Nutrition and Food Hygiene, School of Public Health, Shanxi Medical University, No. 56, Xinjian South Road, Taiyuan, Shanxi 030001, PR China.

The present study was designed to explore the beneficial mitochondrial effects and anti-oxidative activities of plant sterol ester of α-linolenic acid (PS-ALA) through AMP-activated protein kinase (AMPK) signaling in the treatment of nonalcoholic fatty liver disease (NAFLD) using in vivo and in vitro models. The mitochondrial function was evaluated and the oxidative stress index was measured. The protein expression was analyzed by immunohistochemical, immunofluorescence, and western blotting methods. The results showed that PS-ALA significantly suppressed NAFLD and alleviated steatosis in HepG2 cells induced by oleic acid (OA). In addition, PS-ALA promoted mitochondrial biogenesis, enhanced mitochondrial fatty acid oxidation capacity, improved mitochondrial dynamics, and restored mitochondrial membrane potential. Moreover, PS-ALA reduced reactive oxygen species production both in the liver tissue of HFD-fed mice and OA-loaded HepG2 cells. At the molecular level, PS-ALA accelerated the phosphorylation of AMPK and increased the protein expression of peroxisome proliferator-activated receptor-γ co-activator 1α (PGC-1α) and nuclear NF-E2-related factor 2 (Nrf2). Furthermore, the stimulating effects of PS-ALA on the PGC-1α/Nrf1/Tfam pathway and Nrf2/HO-1 pathway as well as its mitochondrial biogenesis promotion effects and anti-oxidative activities were abrogated by the AMPK inhibitor in OA-treated HepG2 cells. In conclusion, the protective effects of PS-ALA on NAFLD appear to be associated with improving mitochondrial function and oxidative stress via activating AMPK signaling.
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http://dx.doi.org/10.1039/d0fo02623aDOI Listing
March 2021

Minimally invasive ileal ureter replacement: Comparative analysis of robot-assisted laparoscopic versus conventional laparoscopic surgery.

Int J Med Robot 2021 Jun 24;17(3):e2230. Epub 2021 Feb 24.

Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, National Urological Cancer Centre, Beijing, China.

Background: This study is an initial comparative analysis of perioperative and intermediate-term functional outcomes between patients who underwent robot-assisted laparoscopic (RALS) or conventional laparoscopic surgery (LS).

Materials And Methods: A total of 25 patients who underwent ileal ureter replacement (10 RALS and 15 LS) were followed by functional cine magnetic resonance urography (MRU) combined with a modified Whitaker test. Also, the characteristics, perioperative data and functional outcomes of the patients were compared.

Results: The estimated blood loss, postoperative hospital stay and time to oral intake were significantly lower in the RALS group. At the median 14-month follow-up, all the patients showed improved renal function and were symptom-free, with no signs of leakage or stenosis observed by cine MRU combined with a modified Whitaker test.

Conclusions: RALS with an extracorporeal bowel resection is feasible and appears to be safe, with quick postoperative recovery and encouraging outcomes.
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http://dx.doi.org/10.1002/rcs.2230DOI Listing
June 2021

Experience managing distal ureteral strictures with Boari flap-psoas hitch and comparison of open and laparoscopic procedures.

Transl Androl Urol 2021 Jan;10(1):56-65

Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.

Background: To summarize our experience with the Boari flap-psoas hitch and compare the indications, perioperative data and outcomes between open and laparoscopic procedures.

Methods: This study retrospectively reviewed 35 patients with complex distal ureteral stricture between January 2015 and April 2019. All patients were treated with Boari flap-psoas hitch by either an open or a laparoscopic procedure. Selection criteria were based on the etiology, comorbidities, medical history, and patient preference.

Results: All surgeries were performed successfully. The median operation time was 201 min (range, 120 to 300 min), and the median estimated blood loss was 50 mL (range, 20 to 400 mL). The median postoperative hospitalization was 9 days (range, 3 to 46 days). Nineteen patients were treated by the open procedure, and 16 were treated by the transperitoneal laparoscopic procedure. The surgical indication of open surgery was broader than that for laparoscopic surgery. For patients experiencing iatrogenic injury and ureterovesical reimplantation failure, no significant differences in sex, laterality, operative time, ASA score or postoperative hospitalization stay were observed between the two groups. The median estimated blood loss was lower in the laparoscopic group than in the open group (P=0.047). Patients in the open group had more surgical complications than patients in the laparoscopic group (P=0.049). The postoperative follow-up showed the radiological resolution of hydronephrosis in 33 patients.

Conclusions: With the appropriate surgical considerations, Boari flap-psoas hitch is a valid method to bridge distal ureteral defects. For select patients, laparoscopic surgery had advantages being a minimal invasive surgery with less estimated blood loss and fewer surgical complications.
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http://dx.doi.org/10.21037/tau-20-789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844529PMC
January 2021

Risk factors of poor prognosis in patients with pyrrolidine alkaloid-induced hepatic sinusoidal obstruction syndrome after transjugular intrahepatic portosystemic shunt.

Hepatol Int 2021 Jan 28. Epub 2021 Jan 28.

Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, 321#, Zhongshan Road, Nanjing, 210008, Jiangsu, China.

Aims: Transjugular intrahepatic portosystemic shunt (TIPS) is an effective method in treating patients with severe hepatic sinusoidal obstruction syndrome induced by pyrrolidine alkaloids (PA-HSOS). However, some patients still have poor postoperative prognosis. So, we aim to evaluate the predictors associated with poor outcomes in PA-HSOS patients receiving TIPS.

Methods: Patients who were diagnosed as PA-HSOS and received TIPS in our hospital between January 2013 and April 2019 were reviewed retrospectively. Baseline information and clinical data were collected. The hazard ratios (HRs) of factors associated with poor prognosis were analyzed by Cox proportional hazard analysis. The Kaplan-Meier method was used to analyze and compare the cumulative incidence of the poor results and survival rate of patients.

Results: During a median of 19.25-month follow-up, death occurred in 17 patients. We found that prothrombin time at baseline with an adjusted HR 1.110 (95% confidence interval 1.014-1.216, p = 0.024) and serum total bilirubin of 9 mg/dl 5 days after TIPS with an adjusted HR 1.114 (95% confidence interval 1.042-1.190, p = 0.001) were independent risk factors for death. The 1-year and 5-year survival rate were 86.2% and 82.1%, respectively. The 1-year survival rate in patients with prothrombin time > 17.85 s at baseline and serum total bilirubin > 9 mg/dl at 5 days after TIPS was significantly lower than that of patients below the corresponding threshold, respectively.

Conclusions: Prolonged prothrombin time at baseline and increased serum total bilirubin levels 5 days after TIPS are independent risk factors for predicting death after TIPS treatment in PA-HSOS patients.
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http://dx.doi.org/10.1007/s12072-020-10126-xDOI Listing
January 2021

Anthocyanins Are Converted into Anthocyanidins and Phenolic Acids and Effectively Absorbed in the Jejunum and Ileum.

J Agric Food Chem 2021 Jan 11;69(3):992-1002. Epub 2021 Jan 11.

Shaanxi Provincial Bioresource Key Laboratory, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, Shaanxi, China.

Anthocyanins have been known for their health benefits. However, the digestion and absorption of anthocyanins through the gastrointestinal tract have not been fully clarified, creating challenges for understanding why anthocyanins have high biological activities and purported low bioavailability . Twenty-seven male rats were intubated with a 500 mg/kg dose of cyanidin-3-glucoside (C3G). Samples from rats' stomach, duodenum, jejunum, ileum, colon, and serum were collected at 0.5, 1, 2, 3, 4, 5, 6, 12, and 24 h after intubation. Three rats without C3G were used as the control with samples collected at 0 h. C3G and its metabolites in each sample were analyzed using high-performance liquid chromatography-PDA-electrospray ionization-MS/MS. These studies' results unequivocally demonstrated that cyanidin and phenolic acids were the primary C3G metabolites absorbed, mainly in the jejunum and ileum, between 1 and 5 h post-ingestion. We speculate that C3G uses phloroglucinaldehyde and protocatechuic acid metabolic pathways in its metabolism .
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http://dx.doi.org/10.1021/acs.jafc.0c07771DOI Listing
January 2021

Spleen Stiffness Predicts Survival after Transjugular Intrahepatic Portosystemic Shunt in Cirrhotic Patients.

Biomed Res Int 2020 13;2020:3860390. Epub 2020 Nov 13.

Department of Gastroenterology, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.

Objectives: Transjugular intrahepatic portosystemic shunt (TIPS) is an effective intervention for portal hypertensive complications. Little is known about the ability of spleen stiffness (SS) for predicting the survival of cirrhotic patients undergoing TIPS. This study is to evaluate the influence of SS detected by point shear wave elastography (pSWE) in predicting survival after TIPS.

Methods: This retrospective cohort study screened consecutive patients who underwent TIPS and reliable pSWE measurement between October 2014 and September 2017 from our prospectively maintained database. SS values were measured before TIPS. The primary endpoint was the overall survival after TIPS. The Cox regression analysis model was used for univariate and multivariate analyses. A receiver operating characteristic (ROC) curve analysis was performed to calculate the sensitivity, specificity, and positive and negative predictive values.

Results: A total of 89 patients were involved in the final analysis. 24 patients (27.0%) died during a median follow-up time of 31 m. Multivariable Cox regression analysis confirmed that higher SS value ( < 0.001), LS value ( = 0.008), diameter of shunt ( = 0.001), and older age ( < 0.001) were independent prognostic factors of survival after TIPS. The risk of death rose 57.440-fold for each SS unit (m/s) increase. SS was also correlated with liver failure after TIPS. ROC analysis showed that the best SS cutoff value was 3.60 m/s for predicting survival, with a sensitivity of 54.2% and specificity of 90.8%.

Conclusions: The SS value determined by pSWE in cirrhotic patients was an independent predictive factor for survival after TIPS.
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http://dx.doi.org/10.1155/2020/3860390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685811PMC
May 2021

Plant Sterol Ester of -Linolenic Acid Attenuates Nonalcoholic Fatty Liver Disease by Rescuing the Adaption to Endoplasmic Reticulum Stress and Enhancing Mitochondrial Biogenesis.

Oxid Med Cell Longev 2019 12;2019:8294141. Epub 2019 Dec 12.

Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Wuhan 430062, China.

Nonalcoholic fatty liver disease (NAFLD) is becoming more common in the world and is presenting a great challenge concerning prevention and treatment. Plant sterol ester of -linolenic acid (PS-ALA) has a potential benefit to NAFLD. To examine the effect of PS-ALA on NAFLD, C57BL/6J mice were given a control diet, high fat and high cholesterol diet (HFD), and HFD plus 2% PS, 1.3% ALA, or 3.3% PS-ALA for 16 weeks. Our results showed that PS-ALA treatment suppressed hepatic steatosis, ameliorated lipid disorder, attenuated inflammatory response, and inhibited oxidative stress. In the molecular level, PS-ALA downregulated high transcriptional and translational levels of endoplasmic reticulum (ER) stress markers ( and ) leading to decreased protein expression of transcription factor and key enzymes involved in de novo lipogenesis ( and ) and cholesterol synthesis ( and ). In parallel, PS-ALA blocked activation and reduced release of IL-1 and IL-18 via inhibiting ER stress-induced sensitization of unfolded protein response sensors ( and ). Finally, PS-ALA improved HFD-induced mitochondrial damage and fatty acid accumulation as exhibited by higher protein and mRNA expression of key genes administering mitochondrial biogenesis (, , and ) and fatty acid -oxidation ( and ). In conclusion, our study originally demonstrated that PS-ALA rescued ER stress, enhanced mitochondrial biogenesis, and thus ameliorated NAFLD.
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http://dx.doi.org/10.1155/2019/8294141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695996PMC
May 2021

Diagnostic value of conventional ultrasound and shear wave elastography in detecting transjugular intrahepatic portosystemic shunt dysfunction.

Acta Radiol 2020 Nov 28:284185120975183. Epub 2020 Nov 28.

Department of Ultrasound, Jinling Hospital, Nanjing Medical University, Nanjing, PR China.

Background: Transjugular intrahepatic portosystemic shunt (TIPS) dysfunction can cause recurrent portal hypertension (PH)-related complications such as ascites and gastroesophageal variceal bleeding. Portography is invasive and costly limits its use as a screening modality.

Purpose: To assess the clinical value of conventional ultrasound in combination with point shear wave elastography (pSWE) to predict TIPS dysfunction.

Material And Methods: A total of 184 patients with cirrhosis scheduled for TIPS implantation were enrolled in this study and evaluated retrospectively. The splenoportal venous blood flow parameter, liver stiffness (LS), and spleen stiffness (SPS) were measured. Outcome measures included differences in portal vein velocity (PVV), splenic vein velocity (SPVV), LS, and SPS. The accuracy of change in PVV (ΔPVV), SPVV (ΔSPVV), and SPS (ΔSPS) to diagnose TIPS dysfunction was investigated.

Results: TIPS dysfunction occurred in 28 of 184 patients (15.2%). Eighteen (64.3%) patients had shunt stenoses and 10 (35.7%) had shunt occlusion. Portal vein diameter (PVD), PVV, splenic vein diameter (SPVD), SPVV, LS, and SPS were not significantly different between the TIPS normal and TIPS dysfunction groups. Compared with the TIPS normal group, PVV and SPVV of the TIPS dysfunction group decreased significantly, whereas SPS increased significantly ( < 0.001). The values of areas under the receiver operating characteristic curves of ΔPVV, ΔSPVV, and ΔSPS for the diagnosis of TIPS dysfunction were 0.97, 0.96, and 0.87, respectively.

Conclusion: pSWE showed a diagnostic efficacy comparable to conventional ultrasound for diagnosing TIPS dysfunction and can be used routinely after TIPS procedures.
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http://dx.doi.org/10.1177/0284185120975183DOI Listing
November 2020

Impact of cancer on mortality and severity of corona virus disease 2019: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Oct;99(44):e23005

The First Clinical Medical College of Lanzhou University, Lanzhou, China.

Background: Cancer patients are in a state of systemic immunosuppression and are considered a highly vulnerable population in the Corona Virus Disease 2019 (COVID-19) epidemic. However, the relationship between cancer and the severity and mortality of patients with COVID-19 remains unclear. This study aims to evaluate whether cancer patients with COVID-19 may be at an increased risk of severe illness and mortality.

Methods: We will perform comprehensive searches in PubMed, EMBASE.com, Web of Science, and the Cochrane Central Register of Controlled Trials to identify studies providing prevalence of cancer between patients with severe and non-severe illness or between non-survivors and survivors. We will use the Newcastle-Ottawa quality assessment scale to assess the quality of included studies. We will conduct pairwise meta-analyses to compute the odds ratio and 95% confidence interval using the Mantel Haenszel method with the random-effects model. The statistical heterogeneity will be assessed using the I statistic. Subgroup analyses, sensitivity analyses, and meta-regression analyses will be performed to explore the sources of heterogeneity.

Results: The results of this study will be published in a peer-reviewed journal.

Conclusion: Our meta-analysis will systematically evaluate the association between cancer and the severity and mortality of patients with COVID-19. This study will provide evidence to help determine whether cancer patients should be provided with special precautions and advised to use stronger personal protection.

Inplasy Registration Number: INPLASY202090093.
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http://dx.doi.org/10.1097/MD.0000000000023005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598814PMC
October 2020

Clinical characterization and outcome of prolonged heart rate-corrected QT interval among children with syndactyly.

Medicine (Baltimore) 2020 Oct;99(42):e22740

Department of Cardiology, Beijing Jishuitan Hosptial, No. 31 East Street, Xinjiekou, XiCheng.

Prolonged heart rate-corrected QT (QTc) interval is an independent risk factor for sudden cardiac death, which is the hallmark of Timothy syndrome (TS). There are little data on children with syndactyly and QTc prolongation.To evaluate the characteristics and long-term outcomes in children with syndactyly, and to attempt to identify TS in patients with syndactyly and QTc prolongation.This is a retrospective case-control study of children with syndactyly who visited Beijing Jishuitan Hospital between July 2003 and February 2013. The patients with prolonged QTc intervals are matched 1:4 with patients without prolongation. Genetic testing of the CACNA1C gene is routinely performed in patients with QTc prolongation.The mean age at admission is 3.4 ± 2.3 years. Compared with the normal QTc group, those with QTc prolongation showed higher frequencies of congenital heart disease (11.8% vs 1.5%, P = .042), mental retardation and facial dysmorphia (11.8% vs 0, P = .004), and T wave alternans (23.5% vs 4.4%, P = .01). In the multivariable analysis, only T wave alternans (OR = 10.61, 95%CI: 1.39-81.16, P = .023) is independently associated with QTc prolongation in patients with syndactyly. One child with QTc prolongation had a mutation in the CACNA1C gene. No patients with prolonged QTs interval met the threshold for TS.Children with syndactyly and prolonged QTc interval had more multisystem diseases and electrocardiography abnormalities. T wave alternans is independently associated with QTc prolongation in patients with syndactyly.
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http://dx.doi.org/10.1097/MD.0000000000022740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571997PMC
October 2020

Comparison of KD-SR-01 robotic partial nephrectomy and 3D-laparoscopic partial nephrectomy from an operative and ergonomic perspective: A prospective randomized controlled study in porcine models.

Int J Med Robot 2021 Apr 24;17(2):e2187. Epub 2020 Nov 24.

Department of Urology, Peking University First Hospital, Beijing, China.

Background: To investigate the feasibility, validity and security of a new robot platform called KD-SR-01 and compare relative merits of KD-SR-01 robotic partial nephrectomy (KD-RPN) and 3D-Laparoscopic Partial Nephrectomy (3D-LPN).

Methods: Twelve porcine models were randomly and equally divided for KD-RPN and 3D-LPN. The perioperative outcomes, physical and mental workload of the surgeon were compared. Physical workload was evaluated with surface electromyography. Mental workload was evaluated with National Aeronautics and Space Administration Task Load Index (NASA-TLX).

Results: All surgeries were performed successfully. The average docking time of KD-RPN was 6 min. The estimated blood loss was lower in KD-RPN (p < 0.05). There were no differences in all the other operative variables (p > 0.05). KD-RPN showed advantages in physical workload and was significantly superior in mental workload (p < 0.05).

Conclusions: KD-RPN is technically feasible, valid and safe in porcine models. It's comparable from operative perspective while KD-RPN had advantages over 3D-LPN from ergonomic perspective.
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http://dx.doi.org/10.1002/rcs.2187DOI Listing
April 2021

Exploring Amino Sugar and Phosphoenolpyruvate Metabolism to Improve -Acetylneuraminic Acid Production.

J Agric Food Chem 2020 Oct 7;68(42):11758-11764. Epub 2020 Oct 7.

State Key Laboratory of Microbial Technology, National Glycoengineering Research Center Shandong University, Jinan 250100, P. R. China.

-acetyl-d-neuraminic acid (NeuAc) has attracted considerable attention because of its wide-ranging applications. The use of cheap carbon sources such as glucose without the addition of any precursor in microbial NeuAc production has many advantages. In this study, improved NeuAc production was attained through the optimization of amino sugar metabolism pathway kinetics and reservation of a phosphoenolpyruvate (PEP) pool in . -acylglucosamine 2-epimerase and -acetylneuraminate synthase from different sources and their best combinations were used to obtain optimized enzyme kinetics and expression intensity, which resulted in a significant increase in NeuAc production. Next, after a design was engineered for enabling the PEP metabolic pathway to retain the PEP pool, the production of NeuAc reached 16.7 g/L, which is the highest NeuAc production rate that has been reported from using glucose as the sole carbon source.
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http://dx.doi.org/10.1021/acs.jafc.0c04725DOI Listing
October 2020

A novel near-infrared fluorescent probe for intracellular detection of cysteine.

Anal Bioanal Chem 2020 Oct 5;412(26):7211-7217. Epub 2020 Aug 5.

College of Chemistry, Jilin University, Qianjin Street 2699, Changchun, 130012, Jilin, China.

Cysteine (Cys) takes part in redox balance in cells as an antioxidant, and imbalance of Cys content in the body can cause a variety of diseases. It is very important to develop a new fluorescent chemosensor to specifically detect Cys intracellular. In this work, a novel NIR fluorescent probe was constructed based on 3-ethyl-1,1,2-trimethyl-1H-benzo[e]indol-3-ium iodide and 5-(4-hydroxyphenyl)furan-2-carbaldehyde. The probe could selectively detect Cys in the presence of homocysteine (Hcy), glutathione (GSH), and other interferences. It also had a number of advantages, including nucleolus-targeting ability, long fluorescence emission wavelength (685 nm), low detection limit (56 nM), and large Stokes shift (172 nm). The probe was employed to enable visualization of Cys in HepG2 cells, and due to its good response in viscous environment, the probe could also locate nucleoli intracellular.
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http://dx.doi.org/10.1007/s00216-020-02853-9DOI Listing
October 2020

Cost analysis of road traffic crashes in China.

Int J Inj Contr Saf Promot 2020 Sep 7;27(3):385-391. Epub 2020 Jul 7.

State Key Laboratory of Automotive Safety and Energy, Tsinghua University, Beijing, China.

Fatalities and injuries resulting from road traffic crashes is always a serious problem. The overall economic losses caused by road traffic crashes are beyond imagination. Including the economic cost of property damage, productivity loss, medical cost, travel delay time cost, legal cost and insurance cost, the total economic cost of traffic crashes in China in 2017 is calculated as 490.1 billion yuan (72.6 billion USD 2017), which is equivalent to 0.60% of the GDP. The cost of productivity loss accounts for the highest proportion of total economic cost, which is 72%. The second is the travel delay cost, accounting for 12% insurance cost, property damage cost and medical cost are followed. The more serious the injury, the higher the unit economic cost. The unit cost of a crash that caused only property damage is 11,274 yuan. The unit cost of a minor injured crash is 20,223 yuan. The highest unit economic cost is the unit cost of a fatal crash, which is 3,181,394 yuan. This study provides important insights into the cost-benefit analysis of China's road safety policies.
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http://dx.doi.org/10.1080/17457300.2020.1785507DOI Listing
September 2020

A novel colorimetric and near-infrared fluorescence probe for detecting and imaging exogenous and endogenous hydrogen peroxide in living cells.

Talanta 2020 Sep 15;217:121000. Epub 2020 Apr 15.

College of Chemistry, Jilin University, Qianjin Street 2699, Changchun, 130012, China. Electronic address:

Hydrogen peroxide (HO), an important member of the family of reactive oxygen species (ROS), has a significant impact on cell signal transduction, energy conversion and immune responses of living organisms. Therefore, accurate detection of the content of HO in living cells is of vital importance. In this paper, we report on the synthesis of a novel colorimetric and near-infrared fluorescent probe HAA, a heterocyclic aromatic amine with acetyl group for the specific detection of both exogenous and endogenous HO in living cells. Our results show that the probe not only possesses high specificity and sensitivity, but also has advantages of low cytotoxicity and good biocompatibility. Theoretical computations elucidated the luminescence and quenching mechanism of HAA in the absence and presence of HO. In addition, HAA was applied to the determination of HO in human serum and the imaging of endogenous HO in living cells, during which it demonstrated excellent performance and good potential for future bioanalysis applications.
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http://dx.doi.org/10.1016/j.talanta.2020.121000DOI Listing
September 2020