Publications by authors named "Han Gao"

373 Publications

Elimination of light chain tailing in reducing capillary electrophoresis with sodium dodecyl sulfate analysis of a monoclonal antibody.

Electrophoresis 2022 Jul 1. Epub 2022 Jul 1.

Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, P. R. China.

Capillary electrophoresis with sodium dodecyl sulfate (CE-SDS) is a common analytical technique for investigating the purity and molecular size heterogeneity of monoclonal antibody (mAb) drugs. In reducing CE-SDS analysis of mAb-A, the light chain peak exhibited severe tailing, seriously affecting the purity analysis. The purposes of this investigation are to clarify the source of tailing and develop a more appropriate CE-SDS method to eliminate light chain tailing. The degree of light chain tailing was closely related to the mAb concentration, SDS concentration, and injection amount, and more hydrophobic detergents such as sodium hexadecyl sulfate (SHS) and sodium tetradecyl sulfate (STS) could be used instead of SDS to obtain better peak shapes. The results also indicated that the tailing was caused by the binding problem associated with SDS, and SHS/STS could provide a more stable and uniform complexation for the light chain. In summary, the method we developed successfully eliminated the light chain tailing and provided a robust characterization of mAb-A in reducing CE-SDS analysis. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/elps.202200134DOI Listing
July 2022

ARL4C Regulates the Progression of Clear Cell Renal Cell Carcinoma by Affecting the Wnt/-Catenin Signaling Pathway.

J Oncol 2022 21;2022:2724515. Epub 2022 Jun 21.

Department of Urology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, China.

Purpose: To investigate the expression of the ADP-ribosylation factor (ARF)-like proteins (ARLs) and ARL4C in clear cell renal cell carcinoma (ccRCC) based on bioinformatics analysis and experimentally determine the effect and mechanism of ARL4C on cellular properties involved in ccRCC progression.

Methods: After downloading the data of cancer patients from the TCGA database, we used various bioinformatics analysis websites and methods to analyze the expression and function of ARLs and ARL4C. The differential expression of ARL4C in clinical renal cancer tissues versus adjacent normal tissues was further verified using immunohistochemistry and real-time quantitative reverse-transcription (qRT-PCR). qRT-PCR was used to explore the expression of ARL4C mRNA in normal renal cells versus different ccRCC cell lines, and the protein expression of ARL4C was further verified using western blotting. CCK-8, colony formation, and EdU assays were used to determine the effect of ARL4C knockdown on ccRCC cell proliferation. We also used wound healing and Transwell assays to analyze the changes in ccRCC cell migration and invasion following ARL4C knockdown. Finally, we used western blotting to probe the molecular mode of action of ARL4C in ccRCC cells after exposure to Wnt signaling pathway agonists.

Results: Biological function analysis showed that methylation of ARL4C and changes in immune cell infiltration and targeted drug sensitivity caused by altered ARL4C expression affected the prognosis of ccRCC. Further bioinformatics analysis suggested that the expression of ARL4C mRNA was increased in ccRCC, and this was associated with a poor prognosis in ccRCC patients. Increased expression of ARL4C was further verified using qRT-PCR and western blotting of human ccRCC tissue samples. Downregulation of ARL4C significantly inhibited the proliferation, migration, and invasion of ccRCC cells, and activation of the Wnt/-catenin pathway promoted the expression of ARL4C. As an essential downstream effector of the Wnt signaling pathway, ARL4C increased the expression of cyclin D1 and c-myc, thereby increasing the ability of the cells to undergo epithelial-mesenchymal transition (EMT) and ccRCC progression.

Conclusions: As a critical factor in the Wnt/-catenin pathway, ARL4C regulates EMT and progression in ccRCC.
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http://dx.doi.org/10.1155/2022/2724515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239764PMC
June 2022

The pathological risk score: A new deep learning-based signature for predicting survival in cervical cancer.

Cancer Med 2022 Jun 28. Epub 2022 Jun 28.

Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, School of Medicine and Engineering, Beihang University, Beijing, China.

Purpose: To develop and validate a deep learning-based pathological risk score (RS) with an aim of predicting patients' prognosis to investigate the potential association between the information within the whole slide image (WSI) and cervical cancer prognosis.

Methods: A total of 251 patients with the International Federation of Gynecology and Obstetrics (FIGO) Stage IA1-IIA2 cervical cancer who underwent surgery without any preoperative treatment were enrolled in this study. Both the clinical characteristics and WSI of each patient were collected. To construct a prognosis-associate RS, high-dimensional pathological features were extracted using a convolutional neural network with an autoencoder. With the score threshold selected by X-tile, Kaplan-Meier survival analysis was applied to verify the prediction performance of RS in overall survival (OS) and disease-free survival (DFS) in both the training and testing datasets, as well as different clinical subgroups.

Results: For the OS and DFS prediction in the testing cohort, RS showed a Harrell's concordance index of higher than 0.700, while the areas under the curve (AUC) achieved up to 0.800 in the same cohort. Furthermore, Kaplan-Meier survival analysis demonstrated that RS was a potential prognostic factor, even in different datasets or subgroups. It could further distinguish the survival differences after clinicopathological risk stratification.

Conclusion: In the present study, we developed an effective signature in cervical cancer for prognosis prediction and patients' stratification in OS and DFS.
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http://dx.doi.org/10.1002/cam4.4953DOI Listing
June 2022

Structural and Functional Analysis of SHP Promoter and Its Transcriptional Response to FXR in Zn-Induced Changes to Lipid Metabolism.

Int J Mol Sci 2022 Jun 10;23(12). Epub 2022 Jun 10.

College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China.

Zinc alleviates hepatic lipid deposition, but the transcriptional regulatory mechanisms are still unclear. In this study, we characterized the promoter of an SHP (short heterodimer partner) in a teleost . The binding sites of an FXR (farnesoid X receptor) were predicted by the SHP promoter, indicating that the FXR mediated its transcriptional activity. The site mutagenesis and the EMSA (electrophoretic mobility shift assay) found that the -375/-384 bp FXR site on the SHP promoter was the functional binding locus responsible for the Zn-induced transcriptional activation. A further study of yellow catfish hepatocytes suggested that the activation of the FXR/SHP is responsible for the effect of Zn on the decreasing lipid content. Thus, this study provides direct evidence of the interaction between the FXR and SHP promoter in fish, and accordingly elucidates the potential transcriptional mechanism by which Zn reduces hepatic lipid accumulation.
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http://dx.doi.org/10.3390/ijms23126523DOI Listing
June 2022

The Inflammatory Response after Moderate Contusion Spinal Cord Injury: A Time Study.

Biology (Basel) 2022 Jun 20;11(6). Epub 2022 Jun 20.

Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense C, Denmark.

Spinal cord injury (SCI) initiates detrimental cellular and molecular events that lead to acute and delayed neuroinflammation. Understanding the role of the inflammatory response in SCI requires insight into the temporal and cellular synthesis of inflammatory mediators. We subjected C57BL/6J mice to SCI and investigated inflammatory reactions. We examined activation, recruitment, and polarization of microglia and infiltrating immune cells, focusing specifically on tumor necrosis factor (TNF) and its receptors TNFR1 and TNFR2. In the acute phase, TNF expression increased in glial cells and neuron-like cells, followed by infiltrating immune cells. TNFR1 and TNFR2 levels increased in the delayed phase and were found preferentially on neurons and glial cells, respectively. The acute phase was dominated by the infiltration of granulocytes and macrophages. Microglial/macrophage expression of increased from 1-7 days after SCI, followed by an increase in , , and , which remained elevated throughout the study. By 21 and 28 days after SCI, the lesion core was populated by galectin-3, CD68, and CD11b microglia/macrophages, surrounded by a glial scar consisting of GFAP astrocytes. Findings were verified in postmortem tissue from individuals with SCI. Our findings support the consensus that future neuroprotective immunotherapies should aim to selectively neutralize detrimental immune signaling while sustaining pro-regenerative processes.
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http://dx.doi.org/10.3390/biology11060939DOI Listing
June 2022

The Function of Nilaparvata lugens (Hemiptera: Delphacidae) E74 and Its Interaction With βFtz-F1.

J Insect Sci 2022 May;22(3)

College of Life Sciences, China Jiliang University, Hangzhou, 310018, China.

Drosophila E74 is an early gene located in the polytene chromosome 74EF puff position. E74 controls the production of late genes, indicating that it plays a crucial role in this cascade model. Nilaparvata lugens E74 is closely related to Diaphorina citri, Bemisia tabaci, and Laodelphax striatellus. After downregulating E74, molting, and nymphal mortality were increased, and ovarian development was delayed. Moreover, the expression of Vg was reduced at the transcriptional level, as measured by qRT-PCR, and the content of Vg protein was reduced, as detected by Western blotting. After downregulating E74, the expression of hormone-related genes, including Tai, βFtz-F1, Met, Kr-h1, UspA, UspB, E93, and Br, was changed. The expression of E74 was significantly decreased after downregulating hormone-related genes. When the expression of E74 and βFtz-F1 was downregulated together, nymph mortality and molting mortality were higher than those when E74 or βFtz-F1 was downregulated alone. Thus, E74 probably interacts with βFtz-F1 at the genetic level. In summary, this study showed that E74 plays a crucial role in the development, metamorphosis and reproduction of N. lugens, possibly via the interaction with βFtz-F1 at the genetic level. This study provides a basis for the development of new target-based pesticides and new methods for the effective control of N. lugens.
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http://dx.doi.org/10.1093/jisesa/ieac041DOI Listing
May 2022

Investigation of an Uncommon Artifact during Reducing Capillary Electrophoresis-Sodium Dodecyl Sulfate Analysis of a Monoclonal Antibody with Dynamic Light Scattering and Reversed Phase High-Performance Liquid Chromatography.

Pharm Res 2022 Jun 14. Epub 2022 Jun 14.

Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.

Purposes: In reducing capillary electrophoresis sodium dodecyl sulfate (CE-SDS) analysis of a monoclonal antibody (mAb-1), the peak area ratio of heavy chain (HC) to light chain (LC) was out of balance, while multiple artifact peaks were observed following the migration of HC. The main purposes of this study were to describe the techniques utilized to eliminate this artifact and clarify the root cause for this interesting phenomenon.

Methods: We optimized the CE-SDS analysis of mAb-1 by a vairety of techniques including changing the concentration of protein or replacing SDS with a more hydrophobic surfactant (i.e., sodium hexadecyl sulfate (SHS) or sodium tetradecyl sulfate (STS) instead of SDS) in sample and/or the sieving gel buffer. Dynamic light scattering (DLS) and reversed phase high-performance liquid chromatography (RP-HPLC) were used to study the protein-surfactant complex.

Results: The artifact could be partially mitigated by reducing the protein concentration and replacing SDS with SHS or STS in the sample and/or the sieving gel buffer solutions. Due to replacing a more hydrophobic surfactant, the HC-surfactant complex formed was more resistant to dissociation, preventing additional hydrophobic HC-HC interaction and aggregation, thus eliminating the artifact problem.

Conclusions: DLS and RP-HPLC are powerful supplementary techniques in characterizing the protein-surfactant complex, and hydrophobic surfactants such as SHS and STS could afford more normal electropherograms during the analysis of mAbs.
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http://dx.doi.org/10.1007/s11095-022-03303-0DOI Listing
June 2022

miR-17-5p/ Is a Potential Driver for Brain Metastasis of Lung Adenocarcinoma Related to Ferroptosis Revealed by Bioinformatic Analysis.

Front Neurol 2022 25;13:878947. Epub 2022 May 25.

Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Objectives: Present study aims to identify the essential mRNAs responsible for the development of brain neurovascular-related metastases (BNM) among lung adenocarcinoma (LUAD) patients. Further, we attempted to predict brain metastases more accurately and prevent their development in LUAD patients.

Methods: Transcriptome data analysis was used to identify differentially expressed mRNAs (DEMs) associated with brain metastasis, and thereby the ferroptosis index (FPI) is calculated using a computational model. Meanwhile, the DEmRNAs linked with FPI, and brain metastasis were derived by the intersection of these two groups of DEMs. We also constructed a ceRNA network containing these DEmRNAs, identifying the /hsa-miR-17-5p/ axis for analysis. Further, a clinical cohort was employed to validate the regulatory roles of molecules involved in the ceRNA regulatory axis.

Results: Here we report the development of a ceRNA network based on BNM-associated DEMs and FPI-associated DEmRNAs which includes three core miRNAs (hsa-miR-338-3p, hsa-miR-429, and hsa-miR-17-5p), three mRNAs (, and ), and five lncRNAs (). Using gene set enrichment analysis (GSEA) and survival analysis, the potential axis of /hsa-miR-17-5p/ was further investigated. It is found that and ferroptosis index are positively correlated while inhibiting tumor brain metastasis. It may be that binds competitively with miR-17-5p and upregulates to increase iron death limiting brain cancer metastases.

Conclusions: The expression of both and is positively correlated with FPI, indicating a possible link between ferroptosis and BNM. According to the results of our study, the ferroptosis-related ceRNA /hsa-miR-17-5p/ axis may contribute to the development of BNM in LUAD patients.
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http://dx.doi.org/10.3389/fneur.2022.878947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174431PMC
May 2022

Highly Diastereoselective Synthesis of γ-Lactams Enabled by Photoinduced Deaminative [3 + 2] Annulation Reaction.

Org Lett 2022 Jun 10;24(24):4365-4370. Epub 2022 Jun 10.

State Key Lab of Urban Water Resource and Environment, School of Science, Harbin Institute of Technology, Shenzhen, Guangdong 518055, China.

The photoinitiated deaminative [3 + 2] annulation reaction of -aminopyridinium salts with alkenes for the synthesis of functionalized γ-lactams is described. This transformation shows good functional group tolerance as well as excellent diastereoselectivity. Preliminary studies suggest that the employed -aminopyridinium salts generate the key amidyl radical intermediates through N-N bond cleavage via a photoinduced single-electron transfer (SET) process. The amidyl radical species would add to the double bond of alkenes, followed by a radical-mediated annulation process, to afford the desired γ-lactams.
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http://dx.doi.org/10.1021/acs.orglett.2c01565DOI Listing
June 2022

Cyanopeptides restriction and degradation co-mediate microbiota assembly during a freshwater cyanobacterial harmful algal bloom (CyanoHAB).

Water Res 2022 Jul 25;220:118674. Epub 2022 May 25.

Key Laboratory of Coastal Environment and Resources of Zhejiang Province, School of Engineering, Westlake University, 18 Shilongshan Road, Hangzhou 310024, China; Institute of Advanced Technology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou 310024, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China. Electronic address:

Cyanobacterial harmful algal blooms (CyanoHABs) are globally intensifying and exacerbated by climate change and eutrophication. However, microbiota assembly mechanisms underlying CyanoHABs remain elusive. Especially, cyanopeptides, as a group of bioactive secondary metabolites of cyanobacteria, could affect microbiota assembly and ecosystem function. Here, the trajectory of cyanopeptides was followed and linked to microbiota during Microcystis-dominated CyanoHABs in Lake Taihu, China. The most abundant cyanopeptide classes detected included microginin, spumigin, microcystin, nodularin and cyanopeptolin with total MC-LR-equivalent concentrations between 0.23 and 2051.54 ppb, of which cyanotoxins beyond microcystins (e.g., cyanostatin B and nodularin_R) far exceeded reported organismal IC and negatively correlated with microbiota diversity, exerting potential collective eco-toxicities stronger than microcystins alone. The microbial communities were differentiated by size fraction and sampling date throughout CyanoHABs, and surprisingly, their variances were better explained by cyanopeptides (19-38%) than nutrients (0-16%). Cyanopeptides restriction (e.g., inhibition) and degradation were first quantitatively verified as the deterministic drivers governing community assembly, with stochastic processes being associated with the interplay between cyanopeptide dynamics and lake microbiota. This study presents an emerging paradigm in which cyanopeptides restriction and degradation co-mediate lake water microbiota assembly, unveiling new insights into the ecotoxicological significance of CyanoHABs to freshwater ecosystems.
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http://dx.doi.org/10.1016/j.watres.2022.118674DOI Listing
July 2022

Computational insights into strain-increase allylborations for alkylidenecyclopropanes.

Chem Commun (Camb) 2022 Jun 21;58(50):7034-7037. Epub 2022 Jun 21.

School of Chemistry and Chemical Engineering, Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, Shandong University, Jinan, Shandong, 250100, China.

The origins of the reactivity of strain-increase allylborations were computationally investigated. The low reactivity of vinylcyclopropyl boronates is due to weak electronic interactions between benzaldehyde and allylboronates. By increasing the acidity of the boron center, the reactivity is significantly improved because the stronger stabilizing O→B interaction effectively compensates for destabilizing steric effects.
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http://dx.doi.org/10.1039/d2cc02264hDOI Listing
June 2022

Relationship between subgroups of central and lateral lymph node metastasis in clinically node-negative papillary thyroid carcinoma.

World J Clin Cases 2022 Apr;10(12):3709-3719

Department of Thyroid and Breast Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Background: Lymph node metastasis (LNM) of papillary thyroid carcinoma (PTC) has a certain regularity and occurs first to the central lymph node and then to the lateral lymph node. The pathway of PTC LNM can guide surgical prophylactic lymph node dissection (LND) for clinical surgeons.

Aim: To investigate the relationship between subgroups of central LNM and lateral LNM in unilateral clinically node-negative PTC (cN0-PTC).

Methods: Data were collected for 1089 PTC patients who underwent surgical treatment at the Department of Endocrine and Breast Surgery of the First Hospital of Chongqing Medical University from January 2016 to December 2017. A total of 388 unilateral cN0-PTC patients met the inclusion criteria and were enrolled in this study. The clinical and pathological data for these 388 patients who underwent total thyroidectomy + central LND + lateral LND were retrospectively analyzed. The relationship between the central LNM and lateral LNM subgroups was investigated.

Results: The coincidence rate of cN0-PTC was only 30.0%.Optimal scaling regression analysis showed that sex (57.1% 42.9%, = 0.026), primary tumor size (68.8% 31.2%, = 0.008), tumor location (59.7% 40.3%, = 0.007), extrathyroid extension (ETE) (50.6% 49.9%, = 0.046), and prelaryngeal LNM (57.1% 42.9%, = 0.004) were significantly associated with ipsilateral level-II LNM. Their importance levels were 0.122, 0.213, 0.172, 0.110, and 0.227, respectively. Primary tumor size (74.6% 30.2%, = 0.016), pretracheal LNM (67.5% 32.5%, < 0.001), and paratracheal LNM (71.4% 28.6%, < 0.001) were significantly associated with ipsilateral level-III LNM. Their importance levels were 0.120, 0.408, and 0.351, respectively. Primary tumor size (72.1% 27.9%, = 0.003), ETE (70.4% 29.6%, = 0.016), pretracheal LNM (68.3% 31.7%, =0.001), and paratracheal LNM (80.8% 19.2%, < 0.001) were significantly associated with ipsilateral level-IV LNM. Their importance levels were 0.164, 0.146, 0.216, and 0.472, respectively.

Conclusion: The LNM pathway of thyroid cancer has a certain regularity. For unilateral cN0-PTC patients with a tumor diameter > 2 cm and pretracheal or ipsilateral paratracheal LNM, LND at ipsilateral level III and level IV must be considered. When there is a tumor in the upper third of the thyroid with prelaryngeal LNM, LND at level II, level III and level IV must be considered.
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http://dx.doi.org/10.12998/wjcc.v10.i12.3709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100740PMC
April 2022

An Unexpected Alteration Colonic Mucus Appearance in the Constipation Model via an Intestinal Microenvironment.

Microsc Microanal 2022 May 30:1-14. Epub 2022 May 30.

Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing 100069, China.

Due to the lack of research between the inner layers in the structure of colonic mucous and the metabolism of fatty acid in the constipation model, we aim to determine the changes in the mucous phenotype of the colonic glycocalyx and the microbial community structure following treatment with Rhubarb extract in our research. The constipation and treatment models are generated using adult male C57BL/6N mice. We perform light microscopy and transmission electron microscopy (TEM) to detect a Muc2-rich inner mucus layer attached to mice colon under different conditions. In addition, 16S rDNA sequencing is performed to examine the intestinal flora. According to TEM images, we demonstrate that Rhubarb can promote mucin secretion and find direct evidence of dendritic structure-linked mucus structures with its assembly into a lamellar network in a pore size distribution in the isolated colon section. Moreover, the diversity of intestinal flora has noticeable changes in constipated mice. The present study characterizes a dendritic structure and persistent cross-links have significant changes accompanied by the alteration of intestinal flora in feces in models of constipation and pretreatment with Rhubarb extract.
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http://dx.doi.org/10.1017/S1431927622000836DOI Listing
May 2022

Ustilaginoidea virens secretes a family of phosphatases that stabilize the negative immune regulator OsMPK6 and suppress plant immunity.

Plant Cell 2022 May 26. Epub 2022 May 26.

Department of Plant Pathology, the Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management, and Joint International Research Laboratory of Crop Molecular Breeding, Ministry of Education, China Agricultural University, Beijing, China.

Rice false smut caused by Ustilaginoidea virens is emerging as a devastating disease of rice (Oryza sativa) worldwide; however, the molecular mechanisms underlying U. virens virulence and pathogenicity remain largely unknown. Here we demonstrate that the small cysteine-rich secreted protein SCRE6 in U. virens is translocated into host cells during infection as a virulence factor. Knockout of SCRE6 leads to attenuated U. virens virulence to rice. SCRE6 and its homologs in U. virens function as a novel family of mitogen-activated protein (MAP) kinase phosphatases harboring no canonical phosphatase motif. SCRE6 interacts with and dephosphorylates the negative immune regulator OsMPK6 in rice, thus enhancing its stability and suppressing plant immunity. Ectopic expression of SCRE6 in transgenic rice promotes pathogen infection by suppressing the host immune responses. Our results reveal a previously unidentified fungal infection strategy in which the pathogen deploys a family of tyrosine phosphatases to stabilize a negative immune regulator in the host plant to facilitate its infection.
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http://dx.doi.org/10.1093/plcell/koac154DOI Listing
May 2022

Improved Active Disturbance Rejection Double Closed-Loop Control of a Rotary-Type VCM in a Moving Mirror Control System.

Sensors (Basel) 2022 May 20;22(10). Epub 2022 May 20.

Aerospace Information Research Institute, Chinese Academy of Sciences, No.9 Dengzhuang South Road, Haidian District, Beijing 100094, China.

Aiming to address the problem of moving mirror speed fluctuations in moving mirror control systems, an improved active disturbance rejection double closed-loop controller (IADR-DCLC) is proposed and verified by simulation to realize the high-performance control of a moving mirror control system. First, the mathematical model of a rotary-type voice coil motor (RT VCM) is established, and the relationship between the angular velocity of the RT VCM and the optical path scanning velocity is analyzed. Second, in order to suppress the model uncertainty and external disturbance of the system, an improved active disturbance rejection controller (IADRC) is proposed. Compared with a conventional ADRC, the tracking differentiator of the proposed IADRC is replaced with desired signal optimization (DSO), and the actual speed is introduced to the extended state observer (ESO). The IADRC is used in the position-speed double closed-loop control model. Finally, the simulation results show that the IADR-DCLC has not only a good tracking effect but also a good anti-interference ability and can meet the requirements of the moving mirror control system for the uniformity of optical-path scanning speed and accurate control of the position of the moving mirror.
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http://dx.doi.org/10.3390/s22103897DOI Listing
May 2022

Clinical significance and correlation of PD-L1, B7-H3, B7-H4, and TILs in pancreatic cancer.

BMC Cancer 2022 May 27;22(1):584. Epub 2022 May 27.

Department of Gastroenterology, The Affiliated Wuxi People's Hospital of Nanjing Medical University, No. 299 Qing Yang Road, Wuxi, Jiangsu Province, 214023, China.

Background: B7 molecules play significant roles in regulating tumor immunity, but their expression patterns and immuno-biological correlations in pancreatic cancer (PaCa) have not been fully discussed.

Methods: RNA-sequencing data of B7 molecules of PaCa samples in the Cancer Genome Atlas (TCGA) dataset was downloaded from the UCSC Xena to assess the expression, correlation, and mutation of the B7 family in PaCa. Next, two PaCa tissue microarrays (TMAs, Cat. HPanA150CS02 and HPanA120Su02) were obtained from Outdo BioTech (Shanghai, China). To detect the expression levels of PD-L1, B7-H3 and B7-H4, immunohistochemistry (IHC) staining was performed on these TMAs.

Results: Most B7 molecules, including B7-1, B7-2, PD-L1, B7-DC, B7-H2, and B7-H5 exhibited similar expression patterns, but B7-H3, B7-H4, B7-H6, and B7-H7 showed outlier expression patterns compared with other B7 molecules. Besides, B7 molecules were genetically stable and exhibited low alteration frequency. IHC staining indicated PD-L1, B7-H3, and B7-H4 were up-regulated in PaCa tissues and showed uncorrelated expression patterns. Furthermore, high expression of PD-L1 and B7-H3 indicated poor-differentiated grades in PaCa. PD-L1 was positively, but B7-H4 was negatively correlated with CD8+ TILs infiltration in PaCa. Moreover, combined PD-L1 and B7-H4 expression was a novel subtyping strategy in PaCa, namely patients with both high PD-L1 and B7-H4 expression exhibited decreased CD8+ TILs infiltration in tumor tissues.

Conclusion: Overall, we systemically analyzed the expression patterns of B7 molecules and proposed a novel subtyping strategy in PaCa. Patients with both high PD-L1 and B7-H4 expression exhibited the immuno-cold phenotype, which may be not suitable for immunotherapy.
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http://dx.doi.org/10.1186/s12885-022-09639-5DOI Listing
May 2022

Plasma and serum exosome markers analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry coupled with electron multiplier.

Talanta 2022 Sep 19;247:123560. Epub 2022 May 19.

National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, PR China. Electronic address:

Although matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple, rapid, and high-throughput assay, its microchannel plate (MCP) detector is limited by the low sensitivity and ion saturation effect when analyzing macromolecules. Herein, we introduced a strategy that combined MALDI-TOF MS with electron multiplier (EM) for the direct analysis of exosomal proteins isolated from human plasma and serum. The results demonstrated that EM yielded a higher sensitivity than MCP detector in high-mass range (m/z 5000-100000). Through the analysis of MALDI-TOF MS coupled with EM, chemokine (C-X-C motif) ligand 12 (CXCL12) ion at m/z 7960 and its degradation products at m/z 7927, 7587, and 7553 were identified as characteristic exosomal proteins in plasma. CXCL4 ion at m/z 7765 was identified as a characteristic protein in serum exosomes. Additionally, the peak intensity of CXCL12 and CXCL4 standards exhibited great linear relationship (CXCL12, R = 0.989; CXCL4, R = 0.986) with the concentrations (ranging from 0.1 to 20 μg/mL) when using EM as detector. In conjunction with ultrasonic assisted matrix coating technology (UAMCT), this assay repeatability in our lab has been excellent with coefficient of variation (CV%) of 4.6% for CXCL12 and 9.3% for CXCL4. Finally, the spectra demonstrated that the intensity of exosome related peaks was significantly enhanced in plasma and serum of patients with Parkinson's disease (PD) (m/z 7553, P < 0.01; m/z 7587, P < 0.01; m/z 7927, P < 0.001; m/z 7980, P < 0.001; m/z 7765, P < 0.01), Alzheimer's disease (AD) (m/z 7553, P < 0.001; m/z 7587, P < 0.001; m/z 7927, P < 0.001; m/z 7980, P < 0.001), and ischemic cerebrovascular disease (ICD) (m/z 7553, P < 0.05; m/z 7587, P < 0.05; m/z 7927, P < 0.01; m/z 7980, P < 0.05; m/z 7765, P < 0.05) compared to that in healthy persons. The fingerprint information of CXCL12 in plasma exosomes has better clinical relevance than serum exosome CXCL4 in MALDI-TOF MS analysis.
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http://dx.doi.org/10.1016/j.talanta.2022.123560DOI Listing
September 2022

Effect of nickel doping on the structure, morphology and oxygen evolution reaction performance of Cu-BTC derived CuCoO.

Dalton Trans 2022 Jun 7;51(22):8757-8765. Epub 2022 Jun 7.

State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, P. R. China.

In this work, nickel (Ni) doped Cu-BTC derived CuCoO (CCO) was successfully synthesized by a solvothermal method, and the effects of Ni doping concentration (such as 1 at%, 3 at% and 5 at%) on the crystal structure, morphology, composition and oxygen evolution reaction (OER) catalytic performance of CuCoO were investigated. X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) were carried out to characterize the crystal structure, morphology and chemical composition of CuCoO crystals. The results show that Ni ions have been successfully doped into the CuCoO crystal structure and this Ni introduction can reduce its grain size, and 5 at% Ni doped CCO (5NCCO) nanosheets exhibit an average particle size of 386 nm with thicknesses around 28 nm. The optimal [email protected] electrode needs an overpotential of 409 mV to generate a current density of 10 mA cm and is able to sustain galvanostatic OER electrolysis for 18 hours with only a minor degradation of 30 mV. The enhanced OER performance may be due to the increase in the catalytic activity area and the improvement in conductivity, which is caused by a decrease in grain size and the formation of a porous structure for Ni doped Cu-BTC derived CuCoO.
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http://dx.doi.org/10.1039/d2dt00970fDOI Listing
June 2022

Combination of FOXD1 and Plk2: A novel biomarker for predicting unfavourable prognosis of colorectal cancer.

J Cell Mol Med 2022 Jun 17;26(12):3471-3482. Epub 2022 May 17.

Department of General Surgery, Ruijin hospital, Shanghai Jiaotong University School of Medicine, Shanghai, PR China.

Colorectal cancer (CRC) is a worldwide disease with worse survival. Our objective is to identify previously unrecognized prognostic factors to better evaluate disease progression. Seven GEO datasets were collected and analysed using R software, followed by KEGG enrichment analysis and TFs network construction. LASSO-COX analysis was performed to select the most useful prognostic features. COX model was used to analyse prognostic factors associated with OS. The survival curve was constructed using Kaplan-Meier analysis. A Nomogram model was also constructed to predict prognosis. A total of 3559 differentially expressed genes (DEGs) and 66 differentially expressed transcription factors were identified. FOXD1 was identified as the most differentially expressed factor of TFs covering the most downstream DEGs and independent risk prognostic factor. Next, FOXD1 expression was detected using immunohistochemical staining in 131 CRC patients' tissue and the association between FOXD1 expression and clinicopathologic features was analysed. High expression of FOXD1 was correlated with TNM stage and pathological differentiation. Multivariate COX regression analyses confirmed that FOXD1 high-expression, TNM stage and tumour differentiation were independent prognostic risk factor of OS and DFS. Patients with high expression of FOXD1 were more likely to have poor overall survival and disease-free survival. The combination of FOXD1 and Plk2 which we have previously reported allowed us to predict the survival of post-surgical CRC patients more accurately, adding to the former prognostic model based on the TNM Stage. The results showed that patients with high expression of both FOXD1 and Plk2 have the worst survival. A combination of FOXD1 and Plk2 can better evaluate patients' survival.
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http://dx.doi.org/10.1111/jcmm.17361DOI Listing
June 2022

Whole genome sequencing of clinical specimens reveals the genomic diversity of porcine reproductive and respiratory syndrome viruses emerging in China.

Transbound Emerg Dis 2022 May 15. Epub 2022 May 15.

Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

The Porcine reproductive and respiratory syndrome virus (PRRSV), a single (+) RNA virus, is characterized by high genome variability and constant evolution. Owing to increasingly complex mutations, there is a growing difficulty in accessing the whole genome. Additionally, there is limited knowledge on PRRSV intra-host nucleotide variants, which may reflect the complex viral-host dynamics. Here, we performed next-generation sequencing on four clinical lung tissues to reveal the genomic diversity and highlight virus-host interactions. The complete genomes of the HN0713 and GDYJ1224 strains shared 90.7% and 91.3% homology with the lineage 1 strain NADC30, respectively, while the GDGZ0408 and GDHY0425 strains shared 92.0% and 91.6% homology with the JXA1 strain, respectively. Recombination analysis showed that the ORF5-7 genes of the GDGZ0408 and GDHY0425 strains, whose complete genomes belong to lineage 8.7 based on the phylogenetic tree, are both recombined with lineage 3 strains. Furthermore, nsp3, nsp10-12, ORF2 genes and a part of the 3'-UTR of the GDHY0425 strain were provided by the lineage 5.1 strain. Two lineage 1 strains (GDYJ1224 and HN0713) were produced by a recombination of lineages 8.7 and 1. Additionally, the lineage 3 strain was associated with the recombinant HN0713 strain. We determined the intra-host single nucleotide variant frequencies and found more than 200 sites at a frequency of >1% in all samples. GDGZ0408 with parts of the nsp9 and nsp10 genes of HP-PRRSV lineage 8.7 presented more genetically diverse populations than others, indicating that lineage 8.7 might drive robust intra-host single nucleotide variants (iSNVs). Moreover, in the iSNV pools, nsp2 and ORF2a presented the highest mutation dynamic. Overall, this study provided evidence for the alarmingly increasing recombination and ever-changing evolutionary dynamics of PRRSV, and revealed the potential causes of vaccine escape, providing a novel insight into the nucleotide variant population in clinical samples.
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http://dx.doi.org/10.1111/tbed.14597DOI Listing
May 2022

Insight into the Role of Psychological Factors in Oral Mucosa Diseases.

Int J Mol Sci 2022 Apr 26;23(9). Epub 2022 Apr 26.

Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing 100069, China.

With the development of psychology and medicine, more and more diseases have found their psychological origins and associations, especially ulceration and other mucosal injuries, within the digestive system. However, the association of psychological factors with lesions of the oral mucosa, including oral squamous cell carcinoma (OSCC), burning mouth syndrome (BMS), and recurrent aphthous stomatitis (RAS), have not been fully characterized. In this review, after introducing the association between psychological and nervous factors and diseases, we provide detailed descriptions of the psychology and nerve fibers involved in the pathology of OSCC, BMS, and RAS, pointing out the underlying mechanisms and suggesting the clinical indications.
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http://dx.doi.org/10.3390/ijms23094760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099906PMC
April 2022

Peroxisome Proliferator-Activated Receptor-α: A Pivotal Regulator of the Gastrointestinal Tract.

Front Mol Biosci 2022 26;9:864039. Epub 2022 Apr 26.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.

Peroxisome proliferator-activated receptor (PPAR)-α is a ligand-activated transcription factor distributed in various tissues and cells. It regulates lipid metabolism and plays vital roles in the pathology of the cardiovascular system. However, its roles in the gastrointestinal tract (GIT) are relatively less known. In this review, after summarizing the expression profile of PPAR-α in the GIT, we analyzed its functions in the GIT, including physiological control of the lipid metabolism and pathologic mediation in the progress of inflammation. The mechanism of this regulation could be achieved interactions with gut microbes and further impact the maintenance of body circadian rhythms and the secretion of nitric oxide. These are also targets of PPAR-α and are well-described in this review. In addition, we also highlighted the potential use of PPAR-α in treating GIT diseases and the inadequacy of clinical trials in this field.
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http://dx.doi.org/10.3389/fmolb.2022.864039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086433PMC
April 2022

THE APPLICATION OF NEURAL NETWORK TECHNOLOGY BASED ON MEA-BP ALGORITHM IN THE PREDICTION OF MICRODOSIMETRIC QUALITIES.

Radiat Prot Dosimetry 2022 Jun;198(7):405-413

School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, PR China.

The most abundant products of the interaction between radiation and matter are low-energy electrons, and the collisions between these electrons and biomolecules are the main initial source of radiation-based biological damage. To facilitate the rapid and accurate quantification of low-energy electrons (0.1-10 keV) in liquid water at different site diameters (1-2000 nm), this study obtained ${\overline{y}}_{\mathrm{F}}$ and ${\overline{y}}_{\mathrm{D}}$data for low-energy electrons under these conditions. This paper proposes a back-propagation (BP) neural network optimized by the mind evolutionary algorithm (MEA) to construct a prediction model and evaluate the corresponding prediction effect. The results show that the ${\overline{y}}_{\mathrm{F}}$ and ${\overline{y}}_{\mathrm{D}}$ values predicted by the MEA-BP neural network algorithm reach a training precision on the order of ${10}^{-8}$. The relative error range between the prediction results of the validated model and the Monte Carlo calculation results is 0.03-5.98% (the error range for single-energy electrons is 0.1-5.98%, and that for spectral distribution electrons is 0.03-4.4%).
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http://dx.doi.org/10.1093/rpd/ncac062DOI Listing
June 2022

Role of the substrate on Ni inhibition in biological sulfate reduction.

J Environ Manage 2022 Aug 9;316:115216. Epub 2022 May 9.

School of Civil Engineering, The University of Queensland, 4072, QLD, Australia.

In treating mine-impacted waters using sulfate-reducing bacteria (SRB), metal inhibition and substrate selection are important factors affecting the efficiency of the bioprocess. This work investigated the role of the substrate (i.e. lactate, formate, glycerol and glucose) on Ni inhibition to SRB with sulfate-reducing activity tests at initial pH 5, 7 and 9 and 100 mg/L of Ni. Results indicated that the type of substrate was a significant factor affecting Ni inhibition in SRB, which was the most negligible in the lactate system, followed by glycerol, glucose, and formate. Although less significant, Ni inhibition also varied with the pH, leading for instance, to a reduction of 77% in the sulfate reducing activity for the formate system, but only of 28% for lactate at pH 5. The added substrate also influenced the precipitation kinetics and the characteristics of the precipitates, reaching Ni precipitation extents above 95%, except for glucose (83.2%).
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http://dx.doi.org/10.1016/j.jenvman.2022.115216DOI Listing
August 2022

Percutaneous delivery of self-propelling thrombin-containing powder increases survival from non-compressible truncal hemorrhage in a swine model of coagulopathy and hypothermia.

J Trauma Acute Care Surg 2022 May 23. Epub 2022 May 23.

Department of Surgery, St. Michael's Hospital and University of Toronto, 30 Bond Street, Toronto, ON, Canada, M5B 1W8.

Background: Non-compressible truncal hemorrhage (NCTH) remains a leading cause of preventable death on the battlefield. Definitively managing severe NCTH requires surgery within the first hour after injury, which is difficult when evacuating casualties from remote and austere environments. During delays to surgery, hemostatic interventions that are performed prehospital can prevent coagulopathy and hemorrhagic shock and increase the likelihood that casualties survive to receive definitive care. We previously reported that a self-propelling thrombin-containing powder (SPTP) can be delivered percutaneously into the abdomen as a minimally invasive intervention and can self-disperse through pooled blood to deliver the hemostatic agents thrombin and tranexamic acid (TXA) locally to noncompressible intracavitary wounds. We hypothesized that in swine with massive NCTH, dilutional coagulopathy and hypothermia, delivering SPTP could extend survival times.

Methods: Ten swine (n = 5 per group) underwent NCTH from a Grade V liver injury following a midline laparotomy. The laparotomy was closed with sutures afterwards, creating a hemoperitoneum, and animals were managed with crystalloid fluid resuscitation, or crystalloid resuscitation and SPTP. SPTP was delivered into the closed abdomen using a CO2-powered spray device and a catheter placed into the hemoperitoneum, entering through the upper right quadrant using the Seldinger technique. Survival to one and three hours was recorded. In an additional animal, hemorrhage was created laparoscopically and SPTP was imaged in-situ within the abdomen to visually track dispersion of the particles.

Results: SPTP dispersed as far as 35 +/- 5.0 cm within the abdomen. SPTP increased survival to one and three hours (Kaplan-Meier p = 0.007 for both). The median survival time was 61 minutes with SPTP and 31 minutes without (p = 0.016).

Conclusion: SPTP effectively disperses medications throughout a hemoperitoneum and increases survival in a model of NCTH. SPTP is a promising strategy for nonsurgical management of NCTH, warranting further testing of its safety and efficacy.

Level Of Evidence: Basic Science, N/A.
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http://dx.doi.org/10.1097/TA.0000000000003670DOI Listing
May 2022

High-Strength, Conductive, Antifouling, and Antibacterial Hydrogels for Wearable Strain Sensors.

ACS Biomater Sci Eng 2022 Jun 5;8(6):2624-2635. Epub 2022 May 5.

Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi 710127, China.

Conductive hydrogels have shown great potential in the field of flexible strain sensors. However, their application is greatly limited due to the poor antifouling and low mechanical strength. Unfortunately, it is still a challenge to improve these two distinct properties simultaneously. Herein, a hydrogel with high strength, good conductivity, and excellent antifouling and antibacterial properties was prepared through the synergistic effect of physical and chemical cross-linking. First, acrylic acid (AA), acrylamide (AM), and 2-methacryloyloxyethyl phosphorylcholine (MPC) monomers were polymerized in the presence of chitosan chains to form the hydrogel. Then, the prepared hydrogel was immersed in a ferric ion solution to further strengthen the hydrogel through ion coordination. The obtained CS-P(AM-MPC-AA)-Fe hydrogel showed outstanding tensile strength (1.03 MPa), excellent stretchability (1075%), good toughness (7.03 MJ/m), and fatigue resistance. The CS-P(AM-MPC-AA)-Fe hydrogel also demonstrated good ion conductivity (0.42 S/m) and excellent antifouling and antibacterial properties. In addition, the strain sensor constructed by the CS-P(AM-MPC-AA)-Fe hydrogel showed high sensitivity and good stability. This work presented a facile method to construct a zwitterionic hydrogel with high-strength, conductive, antifouling, and antibacterial properties, which suggested a promising gel platform for flexible wearable sensors.
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http://dx.doi.org/10.1021/acsbiomaterials.1c01630DOI Listing
June 2022

The potential effectiveness of mixed bacteria-loaded biochar/activated carbon to remediate Cd, Pb co-contaminated soil and improve the performance of pakchoi plants.

J Hazard Mater 2022 08 26;435:129006. Epub 2022 Apr 26.

School of Environmental Science and Engineering, Shaanxi University of Science & Technology, Xi'an 710021, PR China.

Cadmium (Cd) and lead (Pb) are toxic heavy metals that cause severe soil pollution and pose health risks to humans. It is urgent to develop feasible strategies for Pb and Cd remediation. In this study, a bacteria consortium (Enterobacter asburiae G3, Enterobacter tabaci I12 and Klebsiella variicola J2 in a 1:3:3 proportion) with optimal Cd, Pb adsorption ability was constructed and immobilized on biochar (BC)/activated carbon (AC) via physisorption and sodium alginate encapsulation. The effects of mixed bacteria-loaded BC/AC on Cd and Pb remediation were investigated. The results indicated that their application reduced the DTPA-extractable Cd, Pb in soil by 22.05%-55.84% and 31.64%-48.13%, respectively. The residual Pb, Cd were increased while the exchangeable fractions were decreased. Soil urease, catalase and phosphatase activities were enhanced and soil bacterial community was improved, indicating a soil quality improvement. Consequently, the biomass of pakchoi plants was significantly increased. Cd and Pb in the shoots of pakchoi plants were decreased by 28.68%-51.01% and 24.18%-52.87%, respectively. Collectively, the bacteria-loaded BC/AC showed superior performance than free bacteria, BC and AC alone. Our study may provide a better understanding of the development of green and sustainable materials for remediation of heavy metal by the combination of BC/AC and functional bacteria.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129006DOI Listing
August 2022

Comparative analysis of buds transcriptome and identification of two florigen gene AkFTs in Amorphophallus konjac.

Sci Rep 2022 04 26;12(1):6782. Epub 2022 Apr 26.

College of Biosciences and Biotechnology, Hunan Agricultural University, Changsha, 410128, China.

Leaves and flowers of Amorphophallus konjac do not develop simultaneously thus unique features can be elucidated through study of flowering transformation in A. konjac. In this study, transcriptome libraries of A. konjac leaf buds (LB) and flower buds (FB) were constructed followed by high-throughput sequencing. A total of 68,906 unigenes with an average length of 920 bp were obtained after library assembly. Out of these genes, 24,622 unigenes had annotation information. A total of 6859 differentially expressed genes (DEGs) were identified through differential expression analysis using LB as control. Notably, 2415 DEGs were upregulated whereas 4444 DEGs were downregulated in the two transcriptomes. Go and KEGG analysis showed that the DEGs belonged to 44 functional categories and were implicated in 98 metabolic pathways and 38 DEGs involved in plant hormone signal transduction. Several genes were mined that may be involved in A. konjac flower bud differentiation and flower organ development. Eight DEGs were selected for verification of RNA-seq results using qRT-PCR analysis. Two FLOWERING LOCUS T (FT) genes named AkFT1 and AkFT2 were identified though homologous analysis may be the florigen gene implicated in modulation of A. konjac flowering. These genes were significantly upregulated in flower buds compared with the expression levels on leaf buds. Overexpression of AkFT genes though heterologous expression in Arabidopsis showed that the transgenics flowered at a very early stage relative to wild type plants. These findings indicate that AkFT1 and AkFT2 function as regulation genes in A. konjac flowering development and the two genes may present similar functions during flowering transition.
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http://dx.doi.org/10.1038/s41598-022-10817-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043200PMC
April 2022

Coupling of (methane + air)-membrane biofilms and air-membrane biofilms: Treatment of p-nitroaniline wastewater.

J Hazard Mater 2022 08 19;435:128946. Epub 2022 Apr 19.

College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.

Membrane biofilm (MBf) technology is a promising biological water treatment process that combines membrane aeration with biofilms. To expand its application in the treatment of toxic organic wastewater, methane/air gas mixture-MBfs ((CH + Air)-MBfs) and air-MBfs were coupled to enhance the treatment of p-nitroaniline (PNA) wastewater. Based on exploration of the membrane permeability of methane and oxygen, a hybrid MBf reactor was constructed, and the degradation characteristics of PNA and the coupling effects of (CH + Air)-MBfs and air-MBfs were studied. The permeation flux of methane was found to be 1.114 g/(m d) when using a methane/air gas mixture at an aeration pressure of 10 kPa, and this result was better than that when methane was used as the aeration gas alone. Aeration with a methane/air gas mixture provided conditions for realizing aerobic methane oxidation; the aerobic methane oxidation that occurred in the (CH + Air)-MBfs promoted the reduction of PNA, and the intermediates of PNA degradation were further degraded by the air-MBfs. At an influent PNA membrane area load of 1.67 g/(m d), the PNA removal load reached 187.30 g/(m d). The coupling of MBfs took advantage of different matrix-based MBfs and promoted the degradation of PNA by utilizing the synergistic effects of various functional microorganisms.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128946DOI Listing
August 2022

High-Performance Flexible Pressure Sensor with a Self-Healing Function for Tactile Feedback.

Adv Sci (Weinh) 2022 Apr 15:e2200507. Epub 2022 Apr 15.

Center for Nanoscale Characterization and Devices (CNCD), School of Physics and Wuhan National Laboratory for Optoelectronics (WNLO), Huazhong University of Science and Technology (HUST), Wuhan, 430074, P. R. China.

High-performance flexible pressure sensors have attracted a great deal of attention, owing to its potential applications such as human activity monitoring, man-machine interaction, and robotics. However, most high-performance flexible pressure sensors are complex and costly to manufacture. These sensors cannot be repaired after external mechanical damage and lack of tactile feedback applications. Herein, a high-performance flexible pressure sensor based on MXene/polyurethane (PU)/interdigital electrodes is fabricated by using a low-cost and universal spray method. The sprayed MXene on the spinosum structure PU and other arbitrary flexible substrates (represented by polyimide and membrane filter) act as the sensitive layer and the interdigital electrodes, respectively. The sensor shows an ultrahigh sensitivity (up to 509.8 kPa ), extremely fast response speed (67.3 ms), recovery speed (44.8 ms), and good stability (10 000 cycles) due to the interaction between the sensitive layer and the interdigital electrodes. In addition, the hydrogen bond of PU endows the device with the self-healing function. The sensor can also be integrated with a circuit, which can realize tactile feedback function. This MXene-based high-performance pressure sensor, along with its designing/fabrication, is expected to be widely used in human activity detection, electronic skin, intelligent robots, and many other aspects.
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http://dx.doi.org/10.1002/advs.202200507DOI Listing
April 2022
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