Publications by authors named "Hamideh Moravvej"

50 Publications

Evaluation of Fibroblast Viability Seeded on Acellular Human Amniotic Membrane.

Biomed Res Int 2021 24;2021:5597758. Epub 2021 May 24.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Investigating the viability and proliferative rates of fibroblast cells on human amniotic membrane (HAM) as a scaffold will be an important subject for further research. The aim of this study was to assess the fibroblast viability seeded on acellular HAM, since foreskin neonatal allogenic fibroblasts seeded on HAM accelerate the wound healing process.

Methods: Fibroblasts were retrieved from the foreskin of a genetically healthy male infant, and we exploited AM of healthy term neonates to prepare the amniotic scaffold for fibroblast transfer. After cell culture, preparation of acellular HAM, and seeding of cells on HAM based on the protocol, different methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI), and propidium iodide (PI) staining were employed for assessment of fibroblast viability on HAM.

Results: Based on the results obtained from the DAPI and PI staining, the percentage of viable cells in the former staining was clearly higher than that of the dead cells in the latter one. The results of DAPI and PI staining were in accordance with the findings of MTT assay, confirming that fibroblasts were viable and even proliferate on HAM.

Conclusion: Our findings showed the viability of fibroblasts seeded on the acellular HAM using MTT assay, DAPI, and PI staining; however, this study had some limitations. It would be an interesting subject for future research to compare the viability and proliferation rate of fibroblasts seeded on both cellular and acellular HAM.
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http://dx.doi.org/10.1155/2021/5597758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169243PMC
May 2021

Androgenetic alopecia and COVID-19: A review of the hypothetical role of androgens.

Dermatol Ther 2021 May 25:e15004. Epub 2021 May 25.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

The coronavirus disease 2019 (COVID-19) has become the most emerging health issue globally. A prompt investigation regarding disease management and treatment is crucial for decreasing the burden of the disease. Many explorations and hypotheses have been posed, but the definite treatment has not been determined for COVID-19. Recent studies described a substantial prevalence of COVID-19 and also a higher rate of morbidity and mortality in men afflicted with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The gender-related discordance in COVID-19 infection may be due to hormonal differences, socioeconomic factors, genetic susceptibility, gender-related comorbidities, and habits like alcohol consumption. On the other hand, several studies proposed that androgens could improve the immune system and have a protective role in COVID-19, and decreased levels of androgens might be associated with unsatisfactory outcomes. In the field of dermatology, androgenetic alopecia (AGA) is correlated with a hyperandrogenic state and may be related to COVID-19 severity. Furthermore, recent research has assessed the plausible association of AGA and COVID-19. In this review, we investigate all evidence on AGA and its relationship with COVID-19, including the possible role of androgens in COVID-19 severity and outcomes as well as candidate androgen-related drugs for the treatment of COVID-19.
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http://dx.doi.org/10.1111/dth.15004DOI Listing
May 2021

Emerging and Novel Therapies for Keloids: A compendious review.

Sultan Qaboos Univ Med J 2021 Feb 15;21(1):e22-e33. Epub 2021 Mar 15.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Keloids are abnormal fibroproliferative scars with aggressive dermal growth expanding beyond the borders of the original injury. Different therapeutic modalities, such as corticosteroids, surgical excision, topical silicone gel sheeting, laser therapy, cryotherapy, photodynamic therapy and radiotherapy, have been used to treat keloids; however, none of these modalities has proven completely effective. Recently, researchers have devised several promising anti-keloid therapies including anti-hypertensive pharmaceuticals, calcineurin inhibitors, electrical stimulation, mesenchymal stem cell therapy, microneedle physical contact and ribonucleic acid-based therapies. The present review summarises emerging and novel treatments for keloids. PubMed (National Library of Medicine, Bethesda, Maryland, USA), EMBASE (Elsevier, Amsterdam, Netherlands) and Web of Science (Clarivate Analytics, Philadelphia, Pennsylvania, USA) were searched for relevant literature published between January 1987 to June 2020. A total of 118 articles were included in this review. A deeper understanding of the molecular mechanisms underlying keloid scarring pathogenesis would open further avenues for developing innovative treatments.
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http://dx.doi.org/10.18295/squmj.2021.21.01.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968901PMC
February 2021

Therapeutic effects of supplementation with Curcuminoids in critically ill patients receiving enteral nutrition: a randomized controlled trial protocol.

J Diabetes Metab Disord 2020 Dec 26;19(2):1609-1614. Epub 2020 Sep 26.

Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.

Background: Curcuminoids are dietary polyphenols that can improve health indices through different mechanisms such as anti-inflammatory, antioxidant and immunoregulatory properties. Due to the lack of evidences on the efficacy of curcuminoids in critically ill patients, this study was designed to investigate the effects of short-term curcuminoids supplementation on inflammatory, oxidative stress and adipokine indices as well as nutritional and clinical status in Traumatic Brain Injury (TBI) patients admitted in the Intensive Care Unit (ICU).

Methods: The present trial will be performed in the ICU of Sina and Shohadaye Tajrish hospitals of Tehran, Iran. Sixty-two critically ill patients with TBI will be enrolled based on the eligibility criteria. The patients will be randomly assigned into two groups. For 7 days, they will received either 500 mg curcuminoids in combination with 5 mg piperine or matched placebo. A general questionnaire, consent form as well as NUTRIC, SOFA and APACHEII scoring system and anthropometrics will be assessed at baseline. The inflammatory markers including TNF-α, IL-6, MCP-1 and CRP, oxidative stress indices (GPx and SOD) and adipokines (leptin and adiponetctin) will be measured at baseline and at the end of the study. In addition, dietary intake, concomitant drugs and laboratory tests will be recorded daily.

Discussion: To the best of our knowledge, this is the first clinical trial investigating the effect of curcuminoids supplementation in critically ill patient with TBI. The findings of the present study will provide evidence on the efficacy and safety of curcuminoids in these patients.

Trial Registration Number: (http://www.irct.ir, identifier: IRCT20180619040151N1), Registration date:18.09.2018.
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http://dx.doi.org/10.1007/s40200-019-00451-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843870PMC
December 2020

Photoclinic: Cutaneous Metastatic Renal Cell Carcinoma.

Arch Iran Med 2020 12 1;23(12):880-882. Epub 2020 Dec 1.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

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http://dx.doi.org/10.34172/aim.2020.117DOI Listing
December 2020

TH17/IL23 cytokine gene polymorphisms in bullous pemphigoid.

Mol Genet Genomic Med 2020 12 19;8(12):e1519. Epub 2020 Dec 19.

Skin Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: TH17/IL-23 immune axis is considered to be involved in the pathogenesis of autoimmune and chronic inflammatory diseases. Bullous pemphigoid (BP) is the most frequent autoimmune blistering disease, characterized by the presence of autoantibodies against the components of the dermal-epidermal junction. Animal studies and characterization of patient samples point toward a contribution of TH17 cells in BP pathogenesis. However, genetic polymorphisms in the genes of TH17/IL-23 cytokines have not yet been well investigated in BP.

Methods: Detection of polymorphisms in IL-17A (rs2275913 and rs3819025), IL-17F (rs2397084 and rs763780), IL-17RA (rs2229151), and IL-23R (rs2201841, rs7530511, rs11209026, and rs10889677) genes were performed following the collection of blood samples and DNA extraction from BP patients and controls. Gene expression of IL-23R was determined by quantitative RT-PCR analysis.

Results: The prevalence of IL-23R rs7530511 genotypes and alleles, as well as IL-23R rs2201841 alleles, is significantly different between the BP patients and controls. While the minor C-allele of IL-23R rs7530511 is highly present in the patients, the G-allele distribution of IL-23R rs2201841 is significantly more prevalent in the control individuals compared to the BP patients. Genotypes and alleles of other SNPs in IL-17A, IL-17F, and IL-17RA were similarly distributed in patients and controls.

Conclusions: No alteration was found in the gene expression between wild and polymorphic genotypes of IL-23R (rs2201841 and rs7530511) variations, indicating they do not contribute to altering the levels of gene expression in blood. In summary, our data show that the alleles of two SNPs in IL-23R rs2201841 and rs7530511 are associated with BP.
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http://dx.doi.org/10.1002/mgg3.1519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767565PMC
December 2020

Anti-Staphylococcal and cytotoxic activities of the short anti-microbial peptide PVP.

World J Microbiol Biotechnol 2020 Oct 21;36(11):174. Epub 2020 Oct 21.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Over the past years, short anti-microbial peptides have drawn growing attention in the research and trade literature because they are usually capable of killing a broad spectrum of pathogens by employing unique mechanisms of action. This study aimed to evaluate the anti-bacterial effects of a previously designed peptide named PVP towards the clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) in vitro. Secondary structure, cytotoxicity, and membrane-permeabilizing effects of the peptide were also assessed. PVP had a tendency to adopt alpha-helical conformation based upon structural predictions and circular dichroism spectroscopy (in 50% trifluoroethanol). The peptide showed MIC values ranging from 1 to 16 µg/mL against 10 strains of MRSA. In contrast to ciprofloxacin and gentamicin, PVP at sub-lethal concentration (1 µg/mL) did not provoke the development of peptide resistance after 14 serial passages. Remarkably, 1 h of exposure to 4 × MBC of PVP (8 µg/mL) was sufficient for total bacterial clearance, whereas 4 × MBC of vancomycin (8 µg/mL) failed to totally eradicate bacterial cells, even after 8 h. PVP showed negligible cytotoxicity against human dermal fibroblasts at concentrations required to kill the MRSA strains. The results of flow cytometric analysis and fluorescence microscopy revealed that PVP caused bacterial membrane permeabilization, eventually culminating in cell death. Owing to the potent anti-bacterial activity, fast bactericidal kinetics, and negligible cytotoxicity, PVP has the potential to be used as a candidate antibiotic for the topical treatment of MRSA infections.
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http://dx.doi.org/10.1007/s11274-020-02948-6DOI Listing
October 2020

Association between TH2 Cytokine Gene Polymorphisms and Risk of Bullous Pemphigoid.

Immunol Invest 2020 Oct 13:1-13. Epub 2020 Oct 13.

Skin Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

: T-helper 2 (Th2)-associated cytokines are involved in the pathogenesis of bullous pemphigoid (BP), an autoimmune skin disease. Increased expression of Th2 cytokines such as interleukin-4 (IL-4), IL-5, IL-6, IL-10, and IL-13 have been observed in serum, skin biopsies and/or blister fluid. This study aimed to uncover a possible association between Th2 cytokine genetic variations and susceptibility to BP. In a cohort study, blood samples of BP patients and controls were obtained and variations in IL-4 (rs2243250 and rs2070874), IL-4R (rs1805010), IL-5 (rs2069812), IL-6 (rs1800795), IL-10 (rs1800896, rs1800871, and rs1800872), and IL-13 (rs1800925 and rs20541) were genotyped by PCR-RFLP assays. Furthermore, quantitative expression levels of IL-13 gene were evaluated by real-time RT-PCR analysis. Among the studied variations, a significantly higher frequency of the C-allele was observed in IL-13 gene variation (rs1800925) in the healthy individuals than BP patients. This may indicate a protective effect of C-allele on predisposition to BP. Considering individuals carrying polymorphic genotypes compared to wild genotype, the minor G-allele of IL-4R rs1805010 and A-allele of IL-13 rs20541 had a promotive and protective effect, respectively, on predisposing to the development of BP. No significant difference in IL-13 mRNA expression was detected between BP patients and healthy individuals. Our results indicate that IL-13 rs1800925 variation may be a protective genetic marker for the development of BP. Given this preventive effect against BP, therapeutic strategies could potentially be developed interfering with the functions of IL-13 cytokine, which seems to be integral in the pathogenesis of eosinophilic inflammatory disorders, such as BP.
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http://dx.doi.org/10.1080/08820139.2020.1832113DOI Listing
October 2020

Evaluation of the efficacy and safety of cow placenta extract lotion versus minoxidil 2% in the treatment of female pattern androgenetic alopecia.

Int J Womens Dermatol 2020 Sep 4;6(4):318-321. Epub 2020 May 4.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Placenta extract has been shown to improve androgenetic alopecia (AGA) by inducing the anagen phase and increasing hair follicle density and size.

Objective: This study aimed to evaluate the safety and efficacy of cow placenta extract lotion compared with topical minoxidil 2% as a gold standard treatment for female pattern AGA.

Methods: In this double-blind, randomized controlled trial, a total of 90 women with AGA were enrolled and randomly assigned to receive either topical minoxidil 2% or cow placenta solutions. At the end of the sixth month, the number of hair follicles was evaluated using a trichoscope and compared with the baseline. Global photographic review was also conducted by a blinded dermatologist.

Results: By the end of the sixth month, there was an increase in total hair count in the specified area in both groups. The mean increase in hair count was 10.9 ± 5.74 and 10.2 ± 6.5 for minoxidil and cow placenta groups, respectively ( = .63). The percentage of patients who were rated as having moderate or marked growth was 44.2% and 32.2% in the cow placenta and minoxidil groups, respectively ( = .90).

Limitations: The study limitations were the limited number of cases, short duration of treatment, and the fact that none of our participants volunteered to undergo a biopsy to evaluate microscopic changes.

Conclusions: Cow placenta hair-tonic lotion can be as effective as minoxidil 2% for female pattern AGA.
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http://dx.doi.org/10.1016/j.ijwd.2020.04.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522890PMC
September 2020

The quality of life in epidermolysis bullosa (EB-QoL) questionnaire: Translation, cultural adaptation, and validation into the Farsi language.

Int J Womens Dermatol 2020 Sep 3;6(4):301-305. Epub 2020 Jun 3.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Defining the quality of life in the patients with epidermolysis bullosa (EB) is important in patient care and management. Evaluation of quality of life requires a valid and reliable scale. The Quality of Life in Epidermolysis Bullosa (EB-QoL) questionnaire, which is an English 17-item questionnaire, has emerged as a useful tool for assessing the quality of life in the patients with EB.

Objective: This study aimed to evaluate the psychometric properties of the Farsi version of the EB-QoL questionnaire among a group of Iranian patients with EB.

Methods: The Farsi version of the EB-QoL questionnaire was finalized after translation and back-translation. From the 100 patients with EB invited to participate in the study, 83 completed the questionnaire (response rate: 83%). Subsequently, the content validity and construct validity of the questionnaire were assessed. The reliability of the questionnaire was assessed with Cronbach's alpha. Moreover, the correlation between EB-QoL scores and EB severity scores (based on the Birmingham Epidermolysis bullosa severity score scale) was evaluated.

Results: A total of 83 patients (40 male and 43 female) with a median age of 15 years (interquartile range, 9-24 years) and an age range between 3 and 43 years were enrolled in this study. Mean ± standard deviation scores from the EB-QoL questionnaire were 43.7 ± 9.9. The translated EB-QoL questionnaire showed a high internal consistency (Cronbach alpha = 0.90) and adequate item-total correlation. Also, there was a significant correlation between EB-QoL and EB severity scores ( = 0.39;  < .001).

Conclusion: The Farsi version of EB-QoL questionnaire has acceptable validity and reliability. Thus, the questionnaire can be used for future studies to assess the quality of life among Iranian patients with EB.
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http://dx.doi.org/10.1016/j.ijwd.2020.05.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522813PMC
September 2020

Epidermolysis bullosa and the COVID-19 pandemic: challenges and recommendations.

J Dermatolog Treat 2020 Jul 26:1-2. Epub 2020 Jul 26.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

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http://dx.doi.org/10.1080/09546634.2020.1788701DOI Listing
July 2020

Development of keloidal morphea after treatment with cyclosporine in a case of recalcitrant generalized morphea.

Clin Case Rep 2020 May 9;8(5):837-839. Epub 2020 Mar 9.

Skin Research Center Shahid Beheshti University of Medical Sciences Tehran Iran.

Nodular or keloidal morphea, also known as nodular scleroderma, is a rare form of localized sclerosis (SSc) or morphea. In this paper, we reported a case of this rare entity with a review of the literature.
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http://dx.doi.org/10.1002/ccr3.2776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250991PMC
May 2020

IL12B and IL23R polymorphisms are associated with alopecia areata.

Genes Immun 2020 05 1;21(3):203-210. Epub 2020 May 1.

Skin Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Alopecia areata is an autoimmune disease in which activation of autoreactive T cells and inflammatory immune signals target the hair follicles autoantigens. Although cytokines are involved in regulating autoimmune inflammation, the specific involvement of these molecules in the pathogenesis of alopecia areata has been remained unsettled. Here, a possible influence of IL12B, IL17A, and IL23R variations on susceptibility to alopecia areata in Iranian patients was investigated. Genotyping of IL12B (rs3212227), IL17A (rs2275913), and IL23R (rs10889677) variants were performed by extracting genomic DNA from patients and controls. Gene expression was analyzed by real-time RT-PCR. The frequency of IL12B and IL23R gene polymorphisms is significantly higher in the patients than controls, while no significant difference was found for IL17A. Stratification of the patients with respect to age at disease onset indicated that CC genotype of IL12B (rs3212227) and AA genotype of IL23R (rs10889677) gene polymorphisms are significantly associated with late-onset alopecia areata disease. In contrast to IL17A and IL23R, IL12B gene expression levels elevated in patients to that of controls, but genotypes had no effect on levels of gene expression. Overall, our data confirmed that the IL12B and IL23R polymorphisms are associated with the risk to develop alopecia areata in our population.
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http://dx.doi.org/10.1038/s41435-020-0100-1DOI Listing
May 2020

Can Antimicrobial Peptides Be Repurposed as a Novel Therapy for Keloids?

Dermatology 2021;237(2):293-295. Epub 2020 Mar 20.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

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http://dx.doi.org/10.1159/000506831DOI Listing
March 2020

Skin Metastasis of Laryngeal Carcinoma Presenting as Multiple Eruptive Nodules.

Head Neck Pathol 2020 Dec 10;14(4):1154-1157. Epub 2020 Feb 10.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Metastasis of head and neck squamous cell carcinoma (SCC) to the skin of this region is extremely rare and reported in 1-2% of cases. The cutaneous metastases of head and neck cancers often present as multiple papulonodular lesions; however, sporadic cases of solitary or multiple keratoacanthoma-like lesions are reported. We describe a rare case of cutaneous metastases of laryngeal SCC presenting as multiple eruptive keratoacanthoma-like lesions with concomitant scrofuloderma in an area of previous radiotherapy.
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http://dx.doi.org/10.1007/s12105-020-01143-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669965PMC
December 2020

Subcision: Indications, adverse reactions, and pearls.

J Cosmet Dermatol 2020 May 28;19(5):1029-1038. Epub 2020 Jan 28.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Subcision, also known as subcutaneous incisionless surgery, is a surgical intervention used to treat a variety of skin conditions including atrophic acne scars, depressed scars, cellulite, and wrinkles.

Aims: We plan to prepare a review article about the indications of subcision, its adverse reactions, and pearls.

Patients/methods: We searched the literature for the information about subcision and its applications from papers published up to March 2019. The search was performed through the databases of the National Library of Medicine.

Results: After a thorough review of the published papers about subcision, we finally chose to review 40 articles that had proper sample size and design to prepare this review article. Twenty-one papers were related to the application of subcision in the treatment of acne scar and other scars. Four articles were about the application of subcision to treat cellulite, and eight articles were related to other applications of subcision such as the treatment of striae or nasolabial fold. The remaining seven articles include published review papers about subcision or acne scar classification.

Conclusion: Subcision seems to be effective on various skin conditions including acne scar, other depressed scars, and cellulite. It is easy to apply and inexpensive with short downtime and no significant complications. Subcision needs proper technique and adequate experience.
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http://dx.doi.org/10.1111/jocd.13308DOI Listing
May 2020

Quercetin: A potential treatment for keloids.

Sultan Qaboos Univ Med J 2019 Nov 22;19(4):e372-e373. Epub 2019 Dec 22.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

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http://dx.doi.org/10.18295/squmj.2019.19.04.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930037PMC
November 2019

Overlapping and Distinct Gene Polymorphisms in Alopecia Areata in an Iranian Population.

Immunol Invest 2020 Feb 19;49(1-2):204-214. Epub 2019 Nov 19.

Skin Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Alopecia areata (AA) is considered to have a multifactorial etiology and polymorphisms in certain genes have been shown to be associated with AA. Although several reports have investigated the effect of (FASLG) gene variations with predisposing to AA, genetic association of disease, however, varies among different ethnicities and no data have so far been reported in Iranian population. The present study aimed to uncover a possible association between variations in genes and AA. Genomic DNA was extracted from all samples and the SNPs of (rs1800682) and (rs5030772) genes were genotyped in AA patients and controls. In addition, gene expression of was assessed by RT-PCR. Regarding , the frequency of the G-allele was significantly higher in the patients compared to the controls, indicating that the G-allele at this locus could be a risk for developing AA. In contrast, no association was found for rs1800682 () with AA. Similarly, compared to controls, gene expression was upregulated. While no association between clinical-demographic characteristics of the AA patients and their genotypes in variations was observed, multivariate regression analysis indicated a correlation between the incidence of AA disease and its familial history as well as AG/GG genotypes of (rs5030772). In conclusion, our data indicate an association between rs5030772 variation and AA. However, previously reported the association of rs1800682 polymorphism with AA was not observed here. These findings highlight overlapping and distinct genetic polymorphisms in an Iranian cohort which might influence the susceptibility to AA.
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http://dx.doi.org/10.1080/08820139.2019.1688827DOI Listing
February 2020

Melittin: a venom-derived peptide with promising anti-viral properties.

Eur J Clin Microbiol Infect Dis 2020 Jan 17;39(1):5-17. Epub 2019 Aug 17.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Despite tremendous advances in the development of anti-viral therapeutics, viral infections remain a chief culprit accounting for ongoing morbidity and mortality worldwide. Natural products, in particular animal venoms, embody a veritable cornucopia of exotic constituents, suggesting an immensurable source of anti-infective drugs. In this context, melittin, the principal constituent in the venom of the European honeybee Apis mellifera, has been demonstrated to exert anti-cancer, anti-inflammatory, anti-diabetic, anti-infective, and adjuvant properties. To our knowledge, there is no review appertaining to effects of melittin against viruses, prompting us to synopsize experimental investigations on its anti-viral activity throughout the past decades. Accumulating evidence indicates that melittin curbs infectivity of a diverse array of viruses including coxsackievirus, enterovirus, influenza A viruses, human immunodeficiency virus (HIV), herpes simplex virus (HSV), Junín virus (JV), respiratory syncytial virus (RSV), vesicular stomatitis virus (VSV), and tobacco mosaic virus (TMV). However, medication safety, different routes of administrations, and molecular mechanisms behind the anti-viral activity of melittin should be scrutinized in future studies.
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http://dx.doi.org/10.1007/s10096-019-03674-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224078PMC
January 2020

Methylprednisolone pulse therapy plus adjuvant therapy for pemphigus vulgaris: an analysis of 10 years' experience on 312 patients.

Dermatol Ther 2019 09 20;32(5):e13057. Epub 2019 Aug 20.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Steroid pulse therapy has shown satisfactory efficacy and safety in treating pemphigus vulgaris (PV). However, there is a paucity of data about the efficacy and safety of methylprednisolone, despite its frequent administration. The aim of this study is to evaluate the efficacy and safety of steroid pulse therapy in treating PV. In this 10-year retrospective cohort study, 312 patients with PV, who had received methylprednisolone pulse therapy, were included. Data of pulse therapy sessions, adjuvant medications, dosages, remission rates, complications, and mortalities were collected from all patients. A total of 276 patients out of 312 underwent the final follow-up at least 6 months after the last session of pulse therapy. Complete remission off therapy was achieved in 83 patients (30%), and 152 patients (55%) had complete remission on therapy. About 29 (10.5%) patients had lesions of pemphigus at the time of the study follow-up, and 26.8% of remained patients were on the minimal therapy. Methylprednisolone pulse therapy could be considered as an option for proper control of PV in severe cases. It might lead to shorter periods of hospitalization and reduce the need to take long-term high-dose oral steroid therapy.
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http://dx.doi.org/10.1111/dth.13057DOI Listing
September 2019

Cultured Epidermal Melanocyte Transplantation in Vitiligo: A Review Article.

Iran J Public Health 2019 Mar;48(3):388-399

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: The color of the skin is highly heritable but can be influenced by the environments and endocrine factors. Many other factors, sometimes destructive, are also involved in the formation of skin color, which sometimes affects pigmentation patterns. Vitiligo is an autoimmune hypopigmentation painless disorder with appearance of white patches and psychological effects on patients. It is a disease in which melanocytes of the skin are destroyed in certain areas; therefore depigmentation appears.

Methods: We studied more than 60 articles. Several therapeutic methods have been used to return the color of skin in vitiligo. These methods include non-invasive treatment and surgical techniques. Among all these therapies, cell transplantation is an advanced procedure in regenerative medicine. Extraction of melanocytes from normal skin and then their cultivation in the laboratory provides a large number of these cells, the transplanting of which to depigmentation areas stimulates the site to irreversibly produce melanin.

Results: The transplantation methods of these cells have been evolved over many years and the methods of producing blister have been changed to the injection of these cells to the target sites.

Conclusion: In this review, autologous cultured melanocyte transplantation has been considered to be the most viable, safe, and effective method in the history of vitiligo treatments.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570809PMC
March 2019

A comparative study of wound dressings loaded with silver sulfadiazine and silver nanoparticles: In vitro and in vivo evaluation.

Int J Pharm 2019 Jun 24;564:350-358. Epub 2019 Apr 24.

Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.

In the current study, two series of antimicrobial dressings conjugated with silver sulfadiazine (SSD) and silver nanoparticles (AgNPs) were developed and evaluated for chronic wound healing. Highly porous polycaprolactone (PCL)/polyvinyl alcohol (PVA) nanofibers were loaded with different concentrations of SSD or AgNPs and compared comprehensively in vitro and in vivo. SSD and AgNPs indicated a strong and equal antimicrobial activity against S. aureus. However, SSD had more toxicity against fibroblast cells over one week in vitro culture. An in vivo model of wound healing on male Wistar rats was developed with a full thickness wound. All the wound dressings indicated enough flexibility and hydrophilicity, which resulted an adequate adhesion into the wound closure. After 30 days, the control group without any treatment indicated 31% wound closure while the group treated with PCL/PVA (without antimicrobial components) indicated 44% wound closure. Presence of antimicrobial components in the PCL/PVA nanofibers resulted into a lower inflammation response leading to a faster proliferation and maturation phases. In agreement with the higher biocompatibility of AgNPs than SSD, a faster angiogenesis, epithelialization and subsequently, remodeling were observed for the wound dressings loaded with AgNPs. The group treated with the highest concentration of AgNPs showed the fastest healing process leading to a final epithelialization with 96% wound closure after 30 days. This study indicated that AgNPs have higher biocompatibility and regulate wound healing process more efficiently compared to SSD. PCL/PVA nanofibers conjugated with AgNPs are promising wound dressings for full-thickness wounds.
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http://dx.doi.org/10.1016/j.ijpharm.2019.04.068DOI Listing
June 2019

The Association between Genetic Variation in Wnt Transcription Factor () and Alopecia Areata.

Immunol Invest 2019 Aug 23;48(6):555-562. Epub 2019 Apr 23.

a Skin Research Center , Shahid Beheshti University of Medical Sciences , Tehran , Iran.

: Alopecia areata (AA) is a non-scarring hair loss with a polymorphous presentation ranging from patchy lesions to involvement of the entire scalp. The disease is the consequence of an autoimmune attack on hair bulbs that results in a premature transition of hair follicles to catagen and telogen. Thus the Wnt/β-catenin signaling pathway that regulates the hair cycling might be involved in the pathogenesis of AA. Genetic variations in the components of Wnt/β-catenin could greatly alter their adaptive mechanisms against an immunologic attack. : Our aim was to investigate the association between AA and genetic polymorphisms in the TCF7L2 gene, one of the most important components of the Wnt/β-catenin pathway. : This is a case-control study of 145 patients with AA and 152 healthy controls. Genotyping of the gene (rs7903146) was performed via the ARMS-PCR method (amplification refractory mutation system- polymerase chain reaction). The allele and genotype distribution was compared between the two groups. : The frequency of the T allele (0.38 vs. 0.28, odds ratio = 1.56, 95% CI = 1.09-2.17, = 0.013) and TT + CT genotypes (0.68 vs. 0.53, odds ratio = 1.88, 95% CI = 1.17-3.02, = 0.008) were significantly higher in AA patients. : This study indicates that the gene variant is associated with AA. Its contribution to disease pathogenesis could either be through a hair cycling defect or dendritic cell dysregulation.
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http://dx.doi.org/10.1080/08820139.2019.1597109DOI Listing
August 2019

Proinflammatory Cytokine Gene Polymorphisms in Bullous Pemphigoid.

Front Immunol 2019 29;10:636. Epub 2019 Mar 29.

Skin Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Bullous pemphigoid (BP) is a rare autoimmune skin blistering disease, characterized by the presence of autoantibodies against hemidesmosomal autoantigens. Cytokine expression is altered in BP patients, and several of these differently expressed cytokines, including IL-1α, IL-1β, IL-8, and TNF-α, contribute to disease pathogenesis. Since genetic polymorphisms in the genes of these cytokines might be implicated in susceptibility to BP disease, we aimed at testing this implication in susceptibility to BP in an Iranian cohort. Blood samples were collected from the subjects and genomic DNA was extracted. To detect the single nucleotide polymorphisms (SNPs), α (rs1800587), β (rs1143627, rs16944, rs1143634), (rs4073), and α (rs1799964, rs1800630, rs1799724, and rs361525) genes were genotyped in BP patients and healthy controls as well as (rs4073) in pemphigus vulgaris (PV) patients. Quantitative gene expression was evaluated by RT-PCR analysis. A significant difference was observed in the distribution of genotypes or alleles of SNP between the BP patients and controls. The A-allele of SNP is significantly more prevalent in the control individuals compared to the BP patient. To further validate this observation, we included PV patients as an additional control. Again, the A-allele of SNP is significantly more prevalent in the PV compared to the BP patients. While we observed a trend toward significant differences regarding alleles of α rs1799724 as well as alleles of α rs1799964, this difference was, however, not evident after correction for multiple analysis. There was no significant difference in all other studied SNPs. In contrast to α, β, and α, gene expression levels were significantly higher in the patients than that of controls. The minor allele in SNP might play a protective role in susceptibility to BP in Iranian patients. Although higher expression levels of gene was found in the patients compared with healthy controls, these levels, however, suggest no association with the examined polymorphism. Moreover, further investigation revealed an elevation in gene expression between wild and polymorphic genotypes of α rs1800587 and α rs361525 in the patient group and these SNPs are therefore associated with altering the levels of gene expression.
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http://dx.doi.org/10.3389/fimmu.2019.00636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6455081PMC
August 2020

One-pot reactive electrospinning of chitosan/PVA hydrogel nanofibers reinforced by halloysite nanotubes with enhanced fibroblast cell attachment for skin tissue regeneration.

Colloids Surf B Biointerfaces 2019 Jul 27;179:270-279. Epub 2019 Mar 27.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

In this study, in situ glyoxal crosslinked chitosan/poly (vinyl alcohol) (PVA) hydrogel nanofibers reinforced with halloysite nanotubes (HNT) were prepared by the electrospinning method without needing post-treatment for stabilization of the nanofibers in aqueous media. FTIR spectroscopy approved the formation of acetal bonds between glyoxal and hydroxyl groups of PVA and chitosan. Morphological studies by SEM/EDX and TEM in accordance with XRD patterns proved that HNT was successfully incorporated into the crosslinked chitosan/PVA nanofibers. The crosslinked nanofibers were insoluble in water. Due to the hydrophilic nature of HNT, the swelling of the nanofibers was increased from 272% for crosslinked chitosan/PVA nanofibers to around 400% for the HNT reinforced nanocomposite nanofibers. Comparing to chitosan/PVA nanofibers, the tensile strength of the crosslinked nanocomposite nanofibers was increased to 2.4 and 3.5 fold by incorporation of 3 and 5% HNT, respectively. Presence of HNT in chitosan/PVA nanofibers reduced the contact angle with water and increased the hydrophilicity of HNT-reinforced nanofibers favoring the attachment of fibroblast cells. Cytotoxicity studies by AlamarBlue assay showed that presence of HNT increased the biocompatibility of the nanofibers. It was also concluded that glyoxal can be used safely for crosslinking of chitosan/PVA nanofibers without any cytotoxic effect for fibroblast cells. From the results of this work, HNT reinforced chitosan/PVA nanofibers crosslinked by glyoxal are introduced as promising nanomaterials for skin tissue regeneration.
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http://dx.doi.org/10.1016/j.colsurfb.2019.03.054DOI Listing
July 2019

Melittin: from honeybees to superbugs.

Appl Microbiol Biotechnol 2019 Apr 1;103(8):3265-3276. Epub 2019 Mar 1.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

The emergence of antibiotic-resistant bacteria, dubbed superbugs, together with relative stagnation in developing efficient antibiotics has led to enormous health and economic problems, necessitating the need for discovering and developing novel antimicrobial agents. In this respect, animal venoms represent a rich repertoire of pharmacologically active components. As a major component in the venom of European honeybee Apis mellifera, melittin has a great potential in medical applications. In this mini-review, we summarize a multitude of studies on anti-bacterial effects of melittin against planktonic and biofilm-embedded bacteria. Several investigations regarding synergistic effects between melittin and antibiotics were also described. On the whole, the properties of melittin can open up new horizons in a range of biomedical areas, from agriculture to veterinary and clinical microbiology.
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http://dx.doi.org/10.1007/s00253-019-09698-yDOI Listing
April 2019

Chitosan-based biocompatible dressing for treatment of recalcitrant lesions of cutaneous leishmaniasis: A pilot clinical study.

Indian J Dermatol Venereol Leprol 2019 Nov-Dec;85(6):609-614

Department of Polymer Engineering, Amirkabir University of Technology, Tehran, Iran.

Background: Chitosan has a biocompatible, biodegradable and nontoxic nature. The effectiveness of nano-chitosan films in the treatment of cutaneous leishmaniasis has been confirmed previously in susceptible laboratory animals.

Aims: The aim of this study is to evaluate the safety and efficacy of a chitosan-based biocompatible dressing in patients with cutaneous leishmaniasis who were either nonresponsive to or had medical contraindications for conventional treatments.

Materials And Methods: A total of 10 eligible patients were included in this single arm, single center study. The sterile chitosan film was immersed in saline serum and was cautiously extended over the wound to avoid air occlusion. Sterile Vaseline gauze was then applied and the film was kept on the wound site for 7 days and was repeated every week until the healing was completed. Complete clinical response was defined as complete re-epithelialization of the skin lesion as well as microscopic negative results for amastigote forms of Leishmania sp.

Results: All patients showed either significant (30%) or complete (70%) improvement after 8 weeks of therapy and at 16 weeks post treatment all cases were completely cured. It was well tolerated and there were no product-related adverse events such as allergic reaction or infection. Moreover, no recurrences were observed in any patients after 6 months follow-up.

Limitations: The lack of a control group, relatively small sample size and failure to evaluate the histological and molecular effects of chitosan were the limitations of this study.

Conclusion: Our findings confirmed that chitosan can be safely and effectively used for the treatment of cutaneous leishmaniasis. We were unable to find any previous clinical study in evaluating the efficacy of chitosan for cutaneous leishmaniasis on human subjects. Further studies are recommended to design a randomized, double-blinded clinical trial with more volunteers who infected with different species of Leishmania and various clinical forms of cutaneous leishmaniasis.
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http://dx.doi.org/10.4103/ijdvl.IJDVL_189_18DOI Listing
February 2020

Losartan ointment relieves hypertrophic scars and keloid: A pilot study.

Wound Repair Regen 2018 07 25;26(4):340-343. Epub 2018 Oct 25.

Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Keloid and hypertrophic scars are two types of fibrosis caused by extracellular matrix overexpression, and angiotensin II via AT receptor is known to play a key role in stimulation of fibrosis. A pilot placebo controlled single blind study was carried out on patients with hypertrophic scars and keloids. A total of 37 adult volunteers were randomly assigned into losartan 5% or placebo treatment groups. The treatment was performed twice a day for three months and a 6-month follow-up. The treatment was evaluated using Vancouver scar scale method. Totally, 30 participants were analyzed (Losartan ointment n = 20; placebo ointment n = 10; seven placebo volunteers left the study because they thought the treatment was not effective for them). In the losartan group, VSS scores dropped significantly (p < 0.01) both in keloid and hypertrophic scar patients. Vascularity and pliability were significantly reduced by losartan treatment (p < 0.05). It can be concluded that losartan potassium ointment (5%) can alleviate the keloid and hypertrophic scar.
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http://dx.doi.org/10.1111/wrr.12648DOI Listing
July 2018