Publications by authors named "Hamid Reza Monsef-Esfahani"

14 Publications

  • Page 1 of 1

The activity of W.D.J. Koch essential oil against multidrug-resistant .

Iran J Microbiol 2018 Dec;10(6):394-399

Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.

Background And Objectives: Essential oils are used for controlling and preventing human diseases and the application of those can often be quite safe and effective with no side effect. The essential oils have been found to have antiparasitic, antifungal, antiviral, antioxidant and especially antibacterial activity including antibacterial activity against tuberculosis. In this study the chemical composition and anti-TB activity of essential oil extracted from has been evaluated.

Materials And Methods: The essential oil of was obtained by the hydro distillation method and the oil was analyzed by GC-FID and GC-MS techniques. The antibacterial activity of essential oil was evaluated through Minimum Inhibitory Concentration (MIC) assay using micro broth dilution method against multidrug-resistant . The molecular modeling of major compounds was evaluated through molecular docking using Auto Dock Vina against-2-trans-enoyl-ACP reductase (InhA) as key enzyme in cell wall biosynthesis.

Results: The hydrodistillation on aerial parts of yielded 2.5% v/w of essential oil. The major compounds of essential oil were identified as α-terpinenyl acetate (52.85%), β- phellandrene (10.26%) and neocnidilide (10.12%). The essential oil showed relatively good anti-MDR with MIC = 252 μg/ml. The results of Molecular Docking showed that affinity of major compounds was comparable to isoniazid.

Conclusion: The essential oil of aerial parts extracted from was relatively active against MDR , and molecular docking showed the major compounds had high affinity to inhibit 2-trans-enoyl-acyl carrier protein reductase (InhA) as an important enzyme in cell wall biosynthesis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414743PMC
December 2018

Chemical Constituents of (DC.) Boiss., and Evaluation of Its Anti- Activity.

Turk J Pharm Sci 2018 Apr 2;15(1):103-106. Epub 2018 Apr 2.

University of Alabama in Huntsville, Faculty of College of Science, Departments of Chemistry, Huntsville, USA.

Objective: (Apiaceae) is an endemic species in Iran. Up to now, there have been no phytochemical and biological investigations on this species. Therefore, isolation of the main secondary metabolites of the plant along with its anti- activity have been considered in this paper.

Materials And Methods: The dried parts of the plant were extracted with different solvents using solvent percolation and the antibacterial activity of the extracts evaluated by the disk diffusion method. Four compounds were isolated using different column chromatography methods.

Results: The compounds were identified by proton nuclear magnetic resonance and carbon-13 nuclear magnetic resonance as isoquercetin (1), rutin (2), β-sitosterol (3) and 2-decenol (4).

Conclusion: Anti- evaluation of the extracts and isolated compounds against three clinical isolates of revealed that hexane extract of the plant inhibited all strains.
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http://dx.doi.org/10.4274/tjps.96168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227904PMC
April 2018

Phytochemical Analysis and Antioxidant Activity of Aerial Extracts.

Iran Red Crescent Med J 2016 Aug 21;18(8):e24836. Epub 2016 Jun 21.

Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, IR Iran.

Background: Salvia, known as Maryam Goli in the Persian language, is an important genus that includes approximately 900 species in the Lamiaceae family. There are 58 Salvia species growing naturally in Iran, including Salvia . f. and Allen., which grows wild in the northeastern and central parts of the country.

Objectives: This study was designed to determine the chemical composition, in vitro antioxidant activity, and total phenol content of various extracts of .

Materials And Methods: Dried aerial parts of the plant were crushed, then sequentially extracted with n-hexane, ethyl acetate, and methanol. The fractions of were subjected to silica gel column chromatography and Sephedex LH-20. The antioxidant activities of these extracts were measured by ferric reducing antioxidant power (FRAP), and the total phenolic contents of the extracts were evaluated using Folin-Ciocalteu reagent.

Results: The separation and purification processes were carried out using different chromatographic methods. Structural elucidation was on the basis 1H-NMR and 13C-NMR spectral data, in comparison with that reported in the literature. The isolated compounds were salvigenin (1), luteolin (2), cirsiliol (3), β-sitosterol (4), and daucosterol (5). Ethyl acetate extract displayed the highest level of total antioxidants and total polyphenols compared to the other analyzed extracts (n-hexane and methanol). In the FRAP assay, ethyl acetate extract had the highest (230.4±10.5) FRAP value, followed by methanol (211.4 ± 8.3) and n-hexane (143.4 ± 12.04). Total phenol contents were calculated to be 13.8 ± 0.3, 58.25 ± 0.05, and 43.48 ± 0.38 mg of gallic acid/100 g in the n-hexane, ethyl acetate, and methanol extracts, respectively.

Conclusions: The above-mentioned compounds were isolated for the first time from . The antioxidant activity of this plant could be in part related to isolated flavonoids and sterols. The results of this study indicated that could be an important dietary source of phenolic compounds with high antioxidant capacity.
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http://dx.doi.org/10.5812/ircmj.24836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056592PMC
August 2016

A new rearranged tricyclic abietane diterpenoid from Salvia chloroleuca Rech. f. & Allen.

Nat Prod Res 2016 15;30(1):120-4. Epub 2015 May 15.

c Faculty of Pharmacy, Medicinal Plants Research Center, Tehran University of Medical Sciences , Tehran , Iran.

Phytochemical investigation of the roots of Salvia chloroleuca led to the isolation and identification of a new rearranged abietane diterpenoid (1). Its structure was elucidated by interpretation of the 1D and 2D NMR spectra and completed by the analysis of the HR-ESI-MS data. Compound 1 is the secondly reported compound on a rearranged tricyclic abietane (4,5-seco-5,10-friedo-abietane) diterpenoid with a ketal functionality between C-2 and C-11. A plausible biosynthetic pathway of 1 was also proposed.
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http://dx.doi.org/10.1080/14786419.2015.1038536DOI Listing
July 2016

In vitro antioxidant and free radical scavenging activity of four Alkanna species growing in Iran.

Pharmacognosy Res 2015 Jan-Mar;7(1):100-4

Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Background: Medicinal plants are recognized as sources of natural antioxidants that can protect biological system from oxidative stress.

Objective: In this study, the antioxidant activities of four species of the Alkanna genus (Alkanna bracteosa, Alkanna frigida, Alkanna orientalis and Alkanna tricophila) were evaluated.

Materials And Methods: The dried roots of plant samples (100 g) were extracted separately by percolation method with MeOH/H2O (80/20) and four fractions were provided respectively with hexane, chloroform, ethyl acetate, butanol and water. Antioxidant activities were investigated by using 1,1-diphenyl-2-picrylhydrazyl, ferric thiocyanate and thiobarbituric acid methods and compared with quercetin (as positive control).

Results: The results indicated that the butanol fractions of four species had the highest antioxidant activity and radical scavenging activity compared with the other fractions studied (P < 0.05). The 50% effective concentration (half - effective doses) values of butanol fractions are less than quercetin and other fractions, so, these fractions showed potent antioxidant activity. This indicated that the active compounds in the root parts of Alkanna species dissolved in the butanol. All the fractions of four species of Alkanna had antioxidant activity, while, there were no significant differences (P > 0.05) between the total antioxidant activities of same fractions. The total antioxidant activity values of Alkanna fractions in a descending order are as follows: Butanol fraction > ethyl acetate fraction > total extract > hexane fraction > chloroform fraction > aqueous fraction.

Conclusion: The antioxidant activities of butanol fractions of samples were higher than quercetin. This may be because most of the active compounds of Alkanna species dissolved in the butanol.
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http://dx.doi.org/10.4103/0974-8490.147218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285637PMC
January 2015

Antimicrobial effect of the Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (higher Basidiomycetes) and its main compounds.

Int J Med Mushrooms 2014 ;16(1):77-84

Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Mushrooms are considered one of the richest sources of natural antibiotics, and various species of them inhibit the growth of a wide diversity of microorganisms. Ganoderma lucidum, a well-known medicinal mushroom. has many pharmacological and biological activities including an antimicrobial effect, although few studies have investigated the antibacterial and antifungal effects of its purified compounds. The chemical structure of the purified compounds from the hexane fraction was elucidated as ergosta-7,22-dien-3β-yl acetate, ergosta-5,7,22-trien-3β-yl acetate (isopyrocalciferol acetate), ergosta-7,22-dien-3-one, ergosta-7,22-dien-3β-ol, and ergosta-5,7,22-trien-3β-ol (ergostrol). In addition, the structure of ganodermadiol was demonstrated after purification from the chloroform fraction. The fractions inhibited Gram-positive bacteria and yeast, with minimum inhibitory concentration values of 6.25 mg/mL, but were ineffective against Gram-negative bacteria in the tested concentrations. The results were comparable for isolated compounds, whereas the mixture of ergosta-7,22-dien-3β-yl acetate and isopyrocalciferol acetate was weakly effective against Escherichia coli (minimum inhibitory concentration, 10 mg/mL). It could be assumed that the antimicrobial effect of crude fractions is the consequence of mixing triterpenoid and steroid compounds.
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http://dx.doi.org/10.1615/intjmedmushr.v16.i1.70DOI Listing
January 2015

Two matrix metalloproteinase inhibitors from scrophularia striata boiss.

Iran J Pharm Res 2014 ;13(1):149-55

Pharmacognosy and Pharmaceutics Department of Medicinal plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.

Many species belonging to the Scrophularia genus have been used since ancient times as folk remedies for many medical conditions such as scrofulas, scabies, tumors, eczema, psoriasis, inflammations. The aim of this study was to characterize the matrix metalloproteinases (MMPs) inhibitor compounds of the Scrophularia striata extract by bio-guide fractionation. The aerial parts of S. striata were collected and different extracts were sequentially prepared with increasingly polar solvents. The MMPs inhibitory activity of the crude extract and its fractions were evaluated by the Zymoanalysis method. The pure compounds were purified from the active fraction by chromatography methods. Chemical structures were deduced by nuclear magnetic resonance and mass spectrometry. Two active compounds (acteoside and nepitrin) were identified by bio-guide fractionation. The inhibitory effects of nepitrin and acteoside at 20 µg/mL were about 56 and 18 percent, respectivly. The inhibitory effects of acteoside at 80 µg/mL were increased to about 73 percent. In summary, the results suggest that nepitrin effectively inhibited MMPs inhibitory activity at low concentrations, whereas acteoside showed inhibition at high concentrations.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985265PMC
April 2014

Coumarin compounds of Biebersteinia multifida roots show potential anxiolytic effects in mice.

Daru 2013 Jun 27;21(1):51. Epub 2013 Jun 27.

Background: Traditional preparations of the root of Biebersteinia multifida DC (Geraniaceae), a native medicinal plant of Irano-Turanian floristic region, have been used for the treatment of phobias as anxiolytic herbal preparation.

Methods: We utilized the phobic behavior of mice in an elevated plus-maze as a model to evaluate the anxiolytic effect of the plant extract and bio-guided fractionation was applied to isolate the active compounds. Total root extract, alkaline and ether fraction were administered to mice at different doses 30 and 90 min prior to the maze test. Saline and diazepam were administered as negative and positive controls, respectively. The time spent in open and closed arms, an index of anxiety behavior and entry time, was measured as an index of animal activity.

Results: The total root extract exhibited anxiolytic effect which was comparable to diazepam but with longer duration. This sustained effect of the crude extract was sustained for 90 min and was even more after injection of 45 mg/kg while the effect of diazepam had been reduced by 90 min. The anxiolytic effect factor was only present in the alkaline fraction and displayed its effect at lower doses than diazepam while pure vasicinone as the previously known alkaloid did not shown anxiolytic effect. The effect of the alkaline fraction was in a dose dependent manner starting at 0.2 mg/kg with a maximum at 1.0 mg/kg. Bio-guided fractionation using a variety of chromatographic methods led to isolation and purification of three coumarin derivatives from the bioactive fraction, including umbelliferone, scopoletin, and ferulic acid.

Conclusion: For the first time, bio-guided fractionation of the root extract of B. multifida indicates significant sustained anxiolytic effects which led to isolation of three coumarin derivatives with well-known potent MAO inhibitory and anti-anxiety effects. These data contribute to evidence-based traditional use of B. multifida root for anxiety disorders.
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http://dx.doi.org/10.1186/2008-2231-21-51DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707806PMC
June 2013

Benefit of Aloe vera and Matricaria recutita mixture in rat irritable bowel syndrome: Combination of antioxidant and spasmolytic effects.

Chin J Integr Med 2012 Dec 21. Epub 2012 Dec 21.

Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, 1417614411, Iran.

OBJECTIVE: To evaluate the beneficial effects of a mixture of Aloe vera (AV) and Matricaria recutita (German chamomile, GC) in an experimental model of irritable bowel syndrome (IBS). METHODS: IBS was induced by a 5-day restraint stress in rats including the groups of control (water), GC (300 mg/kg), loperamide (10 mg/kg), mixed AV and GC (50: 50 at doses of 150, 300 or 450 mg/kg assigned as Mix-150, Mix-300 and Mix-450, respectively) and the sham group which did not receive any restraint stress and was fed with saline. All medications were administered intragastrically by gavage for 7 days, 2 days as pre-treatment followed by 5 days during induction of IBS every day before restraining. RESULTS: The increased tumor necrosis factor alpha (TNF-α), myeloperoxidase (MPO) activity, and lipid peroxidation (LPO) in colonic cells in the control group were significantly decreased in the treatment groups. GC inhibited only small bowel transit while the AV/GC mixture delayed gastric emptying at the doses of 150 and 300 mg/kg. The AV/GC mixture also reduced colonic transit and small bowel transit at the dose of 150 mg/kg. CONCLUSIONS: The severity of stress-induced IBS was diminished by the AV/GC mixture at all doses used but not dose-dependently, via inhibiting colonic MPO activity and improving oxidative stress status. The effect of the mixture was more effective than GC alone. The present results support effectiveness of the AV and GC combination in IBS.
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http://dx.doi.org/10.1007/s11655-012-1027-9DOI Listing
December 2012

Comparative Chemical Composition and Antioxidant Properties of the Essential Oils and Aromatic Water from Teucrium persicum Boiss.

Iran J Pharm Res 2012 ;11(2):573-81

Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.

The essential oils and aromatic water, known as Arak in traditional Iranian medicine, comes from the aerial part of Teucrium persicum Boiss., which is grown in Fars Province located in Iran. The samples were collected in summer and the oils and aromatic water were obtained through steam distillation. The chemical composition of the oils was analyzed using GC-MS. An analysis of the chemical profile of the isolated oils revealed the presence of more than 80 compounds, mainly oxygenated monoterpenes and sesquiterpene hydrocarbons. The principal components of essential oil were α-cadinene (9.7%), 1,4-cadinadiene (9.2%) and α-terpinyl acetate (7.9%). The major constituents in the Arak were determined to be linalool (10.4%), α-cadinene (7.5%) and γ-terpineol (7.3%). Most of the compounds identified from different oils were similar, but their amounts differed. The oil revealed a higher content of total phenolics than the Arak (1.71 ± 0.12 mg GAE/g DW and 1.36 ± 0.11 mg GAE/g DW, respectively). The antioxidant activity of the oils was calculated by using a ferric reducing antioxidant power assay (FRAP), DPPH radical scavenging activity, and a reducing power assay (RP). The FRAP value points to a considerably higher reducing power of essential oil (220 ± 7.2 µmol Fe(2+)/g DW) compared to that of Arak (113 ± 5.4 µmol Fe(2+)/g DW). Essential oil exhibited higher radical scavenging potential (IC50 = 0.29 mg/mL) than Arak (IC50 = 4.19 mg/mL). The reducing power of essential oil (51.7 ± 4.3 µg BHA/g DW) was higher than that of Arak (34.1 ± 2.7 µg BHA/g DW). The studied essential oils showed good antioxidant activities, which were higher than those of Arak.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832155PMC
November 2013

Anti-inflammatory and analgesic activity of Alkanna bracteosa and Alkanna tricophila.

Nat Prod Res 2012 27;26(6):564-9. Epub 2011 Jul 27.

Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Alkanna bracteosa and Alkanna tricophila, Boraginaceae, have been reported to be useful for their anti-inflammatory and wound-healing effects in traditional medicine. Methanol extracts of A. bracteosa and A. tricophila were evaluated for their potential analgesic and anti-inflammatory properties. Alkanna bracteosa was observed to produce a maximum of 42% reduction of hind paw licking in acute as well as 68% alleviation in inflammatory phase of formalin test in mice and about 30% declination of carrageenan-induced rats paw swelling at doses 100-400 mg kg⁻¹ in comparison to negative control; on the other hand, A. tricophila required at least 200 mg kg⁻¹ to exhibit a significant reduction in paw licking or oedemas at the early phase of formalin test and the late phase of carrageenan test, respectively. Our results suggest that the extracts may be of use for their analgesic and anti-inflammatory effects.
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http://dx.doi.org/10.1080/14786419.2010.532795DOI Listing
June 2012

On the benefit of Teucrium in murine colitis through improvement of toxic inflammatory mediators.

Hum Exp Toxicol 2010 Apr 9;29(4):287-95. Epub 2010 Feb 9.

Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Iran.

Regarding the role of free radicals in pathogenesis of inflammatory bowel disease (IBD), we were interested to investigate the effects of Teucrium persicum with approved antioxidant and anti-inflammatory properties in an experimental model of colitis. Immunologic colitis was induced by rectal administration of a mixture of 2,4,6-trinitrobenzene sulphonic acid (TNBS) and ethanol through rubber cannula into rats. Three different doses of Teucrium (100, 200, and 400 mg/kg) were gavaged in a duration of 10 days to rats. Endpoint markers of colitis included macroscopic and microscopic examination of colon tissue and measuring colonic cells concentrations of tumor necrosis factor-alpha (TNF-alpha), interlukin-1beta (IL-1beta), total antioxidant power as ferric reducing antioxidant power (FRAP), myeloperoxidase (MPO), and lipid peroxidation as thiobarbitoric acid-reactive substance (TBARS). Teucrium at all doses improved both macroscopic and histological damages of rats with colitis. Teucrium reduced colonic MPO activity and concentrations of cellular lipid peroxides, TNF-alpha, and IL-1beta, with a concomitant increase in FRAP value in rats with colitis. It is concluded that beneficial effects of Teucrium in experimental colitis is mediated through its antioxidant and anti-inflammatory potentials. Examination of this herbal medicine in patients with IBD as a supplement would further reveal the potential of Teucrium.
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http://dx.doi.org/10.1177/0960327110361754DOI Listing
April 2010

Efficacy of purslane in the treatment of oral lichen planus.

Phytother Res 2010 Feb;24(2):240-4

Department of Oral Medicine, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

This study evaluated the effectiveness of antioxidant-rich purslane in the treatment of oral lichen planus (OLP). A total of 37 biopsy-proven symptomatic OLP patients were selected for this randomized double-blind placebo-controlled trial. All subjects were divided into two groups to receive purslane (n = 20) or placebo (n = 17) for 3 months. Assessments were made at baseline, after 2 weeks and each month for 6 months, based on the visual analog scale (VAS) and clinical improvement including lesion type and size. Approximately 83% of the purslane patients showed partial to complete clinical improvement but 17% had no response. In the placebo group 17% experienced partial improvement, 73% did not respond and 10% showed worsening. According to VAS scores, a partial to complete response was observed in all purslane-treated patients, while 71%, 15% and 14% of the controls demonstrated partial response, no response and worsening of the symptoms, respectively. A significant decrease in VAS scores was seen at the end of the study period (p < 0.001). No serious side-effects occurred in either of the groups. According to our findings purslane is clinically effective in the treatment of OLP. Considering the lack of side-effects during the study period, it may be a favorable alternative treatment for OLP.
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http://dx.doi.org/10.1002/ptr.2919DOI Listing
February 2010

Studies on the microbial transformation of androst-1,4-dien-3,17-dione with Acremonium strictum.

J Ind Microbiol Biotechnol 2006 Sep 31;33(9):725-33. Epub 2006 May 31.

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

The strain of Acremonium strictum PTCC 5282 was applied to investigate the biotransformation of androst-1,4-dien-3,17-dione (I; ADD). Microbial products obtained were purified by preparative TLC and the pure metabolites were characterized on the basis of their spectroscopic features (13C NMR, 1H NMR, FTIR, MS) and physical constants (melting points and optical rotations). The 15 alpha-Hydroxyandrost-1,4-dien-3,17-dione (II), 17 beta-hydroxyandrost-1,4-dien-3-one (III), androst-4-en-3,17-dione (IV; AD), 15 alpha-hydroxyandrost-4-en-3,17-dione (V), 15 alpha,17 beta-dihydroxyandrost-1,4-dien-3-one (VI) and testosterone (VII) were produced during this fermentation. Formation of the 15 alpha,17 beta-dihydroxy derivative of ADD is reported for the first time during steroid biotransformation. The bioconversion reactions observed were 1,2-hydrogenation, 15 alpha-hydroxylation and 17-ketone reduction. From the time course profile of this biotransformation, ketone reduction and 1,2-hydrogenation were observed from the first day of fermentation while 15 alpha-hydroxylation occurred from the third day. Optimum concentration of the substrate, which gave the maximum bioconversion efficiency, was 0.5 mg ml(-1) in one batch. The highest yield of the microbial products recorded in this work was achieved within the pH range 6.5-7.3 and at the temperature of 27 degrees C.
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http://dx.doi.org/10.1007/s10295-006-0135-yDOI Listing
September 2006