Publications by authors named "Hamid Mollahajian"

3 Publications

  • Page 1 of 1

Three common CARD15 mutations are not responsible for the pathogenesis of Crohn's disease in Iranians.

Hepatogastroenterology 2010 Mar-Apr;57(98):275-82

Digestive Disease Research Center (DDRC), Shariati Hospital, Tehran University, Medical Sciences North Kargar Street, Tehran, Iran 14117-13135.

Background/aims: Crohn's disease frequency has increased in recent years in Iran. Genetic and environmental factors predispose people to this disease. Mutation in Caspase Recruitment Domain 15 (CARD15) gene is the most well known genetic predisposing factor to this disease. Frequency of three common CARD15 mutations has been studied in different ethnic groups. We aimed to study the frequency of these mutations in Iranian patients affected with Crohn's Disease.

Methodology: One hundred fifteen proved cases of Crohn Disease and 115 age and sex matched normal controls were recruited in this study. Lf1007fs, R702W and G908R mutations were studied by Polymerase Chain Reaction-Restriction Fragment Length Polymorphims (PCR-RFLP) followed by sequencing the positive cases.

Results: Lf1007fs and G908R mutations were not found in either patients or age-sex matched controls. Just in two patients, R702W mutation was proved by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and sequencing. None of these patients had illeal or fibrostenotic type of disease while 14.7% of total patients had stricturing type of disease. No complication was seen in these two patients while 50.4% of patients had acquired complications during the course of disease.

Conclusion: The three mutations described are not responsible for the pathogenesis of Crohn's Disease in Iranians. The results are in accordance with other Asian nations' studies on IBD Patients.
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August 2010

Nail molybdenum and zinc contents in populations with low and moderate incidence of esophageal cancer.

Arch Iran Med 2008 Jul;11(4):392-6

Jostar Research Group, Students' Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Background: The objective of our study was to compare the zinc and molybdenum contents of nails in two populations at high and low risk for esophageal cancer in northern Iran.

Methods: Four groups were studied including a control group in Tehran--a low-risk area (n=20); a group of patients with esophageal cancer from Sari--a moderate- risk region in northern Iran (n=20); a group of patients' family members (n=20); and a control group in Sari (n=40). Molybdenum and zinc levels were measured in their nail samples using flame atomic absorption spectrometry.

Results: Both molybdenum and Zinc were significantly (P<0.01) lower in the Sari control group (molybdenum: 0.472 ppm, zinc: 173.0 ppm) compared with the Tehran control group (molybdenum: 0.740 ppm, zinc: 251.5 ppm). These elements were lower in content in patients with esophageal cancer (molybdenum: 0.283 ppm, zinc: 126.5 ppm) compared with the Sari control group (P<0.05 for molybdenum and P<0.001 for zinc). Only zinc was significantly (P<0.001) lower in patients compared with their family members (molybdenum: 0.456 ppm, zinc: 175.5 ppm). No differences were found between the Sari control group and the patients' family members in any of the two elements.

Conclusion: Zinc and molybdenum levels are much lower in north of Iran--a moderate- risk area for esophageal cancer--compared with Tehran--a low-risk area. Based on these results, more investigations on the relations of trace elements with esophageal cancer in high-risk areas in northern Iran are warranted.
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July 2008

Prevalence of human papillomavirus genotypes in women with normal and abnormal cervical cytology in Iran.

Asian Pac J Cancer Prev 2006 Oct-Dec;7(4):529-32

Department of Genetics and Genomics, Cancer Research Center, Tehran University of Medical Sciences, Gorgan, Iran.

Introduction: HPV infection has a prime etiologic role in development and progression of cervical cancer, one of the most frequent forms of cancer among women in developing countries. This study was designed to determine the most prevalent HPV genotypes in women with normal and abnormal cervical cytology in Iran.

Materials And Methods: Samples from134 patients, including 127 who attended gynecology clinics and 7 with solid cervical tumors were used. All 127 patients underwent routine Pap tests for cytological evaluation and at the same visit a sample of cervical epithelial cells was obtained by scraping the cervix osteum. In each case HPV infection was primarily evaluated by PCR using GP 5/6 primers and then subtyping was performed in proved infected samples with specific primers for HPV 16, 18, 31, 33, 11 and 6. After cytological evaluation, 50 patients with abnormal Pap tests were categorized as the abnormal group and the remaining 77 patients as the normal group.

Results: In the normal group, HPV infection was established in 10 cases (13% infection rate), while 30 HPV positive cases were discovered in the abnormal group (60% infected). The most prevalent genotypes among the infected samples were HPV 16 (76%), HPV18 (12.7%) and HPV11/6 (8.5%). Moreover, all 7 tumor samples were positive for HPV general primers of which, 5 samples were infected with HPV 16, two were co-infected with HPV16,18 and HPV16,31 genotypes and one was infected with HPV 18.

Conclusions: Infection with HPV 16 was found to be significantly higher in abnormal group in comparison with normal group (42% vs. 11.6%, P value <0.005), likewise HPV18 genotypes were proved to be more prevalent in abnormal group (8% vs. 0%, P value <0.05). No significant relation between other HPV genotypes and pathologic cervical changes was obtained. According to our study high rates of infection with HPV genotypes in sexually active Iranian women makes molecular investigation for HPV16 and 18 very essential in clinical approaches to patients with proven dysplasia in their screening tests and also for those patients with borderline (i.e. ASCUS) or incongruous pathology reports. Larger studies are required to determine the most appropriate vaccine with highest protection in Iranian women.
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March 2007