Publications by authors named "Haiyun Guo"

15 Publications

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Single-shot digital phase-shifting Moiré patterns for 3D topography.

Appl Opt 2021 Feb;60(4):A84-A92

A simple and robust technique of Moiré topography with single-image capture and incorporating digital filtering along with a four-step digitally implemented phase-shifting method is introduced for three-dimensional (3D) surface mapping. Feature details in the order of tens to hundreds of microns can be achieved using interferometrically generated structured light to illuminate the object surface. Compared to the traditional optical phase-shifting method, a digital phase-shifting method based on Fourier processing is implemented with computer-generated sinusoidal patterns derived from the recorded deformed fringes. This enables a single capture of the image that can be used to reconstruct the 3D topography of the surface. Single-shot imaging is simple to implement experimentally and avoids errors in introducing the correct phase shifts. The feasibility of this technique is verified experimentally, and applications to metallic surfaces are demonstrated.
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http://dx.doi.org/10.1364/AO.404424DOI Listing
February 2021

Rosmarinic acid protects rats against post-stroke depression after transient focal cerebral ischemic injury through enhancing antioxidant response.

Brain Res 2021 Apr 4;1757:147336. Epub 2021 Feb 4.

Department of Anesthesiology, Xijing Hospital, the Fourth Military Medical University, Xi'an, Shaanxi 710032, China. Electronic address:

Rosmarinic acid (RA), a natural polyphenol, possesses potent antioxidant and anti-inflammatory activities. To evaluate the ability of RA to cure ischemic stroke and post-stroke depression (PSD), rats were treated with various doses of RA after cerebral ischemia. Neurological deficits and infarct volume of the brain were measured. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) were examined at different time points. In addition, a forced swimming test and sucrose preference test were performed to detect the anti-depressive effects of RA. Our results revealed RA administration significantly alleviated neurological deficits and reduced infarct volumes. RA attenuated the decrease of SOD, CAT activities and GSH levels in the ischemic penumbra of the brain. Most importantly, RA treatment alleviated the depression behaviors. Increased expression of Nrf2 was also induced by RA, while down regulation Nrf2 by Nrf2-short-hairpin RNA sequences reversed the increasing activity of SOD and CAT induced by RA, as well as the protection against PSD. The present study indicates that RA exerts a potent neuroprotective effect against stroke and PSD, which could be a promising therapeutic intervention for stroke.
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http://dx.doi.org/10.1016/j.brainres.2021.147336DOI Listing
April 2021

Glycogenolysis Is Crucial for Astrocytic Glycogen Accumulation and Brain Damage after Reperfusion in Ischemic Stroke.

iScience 2020 May 6;23(5):101136. Epub 2020 May 6.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China; Translational Research Institute of Brain and Brain-Like Intelligence & Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital Affiliated to Tongji University School of Medicine, Shanghai 200081, China. Electronic address:

Astrocytic glycogen is an important energy reserve in the brain and is believed to supply fuel during energy crisis. However, the pattern of glycogen metabolism in ischemic stroke and its potential therapeutic impact on neurological outcomes are still unknown. Here, we found extensive brain glycogen accumulation after reperfusion in ischemic stroke patients and primates. Glycogenolytic dysfunction in astrocytes is responsible for glycogen accumulation, caused by inactivation of the protein kinase A (PKA)-glycogen phosphorylase kinase (PhK)-glycogen phosphorylase (GP) cascade accompanied by the activation of glycogen synthase kinase-3β (GSK3β). Genetic or pharmacological augmentation of astrocytic GP could promote astrocyte and neuron survival and improve neurological behaviors. In addition, we found that insulin exerted a neuroprotective effect, at least in part by rescuing the PKA-PhK-GP cascade to maintain homeostasis of glycogen metabolism during reperfusion. Together, our findings suggest a promising intervention for undesirable outcomes in ischemic stroke.
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http://dx.doi.org/10.1016/j.isci.2020.101136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240195PMC
May 2020

Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes.

Cell Mol Neurobiol 2020 Jul 20;40(5):801-812. Epub 2019 Dec 20.

Department of Neurobiology, The School of Basic Medicine, The Fourth Military Medical University, Xi'an, China.

Cerebral glycogen is principally localized in astrocytes rather than in neurons. Glycogen metabolism has been implicated in higher brain functions, including learning and memory, yet the distribution patterns of glycogen in different types of astrocytes have not been fully described. Here, we applied a method based on the incorporation of 2-NBDG, a D-glucose fluorescent derivative that can trace glycogen, to investigate glycogen's distribution in the brain. We identified two types of astrocytes, namely, 2-NBDG (glycogen-deficient) and 2-NBDG (glycogen-rich) cells. Whole-cell patch-clamp and fluorescence-activated cell sorting (FACS) were used to separate 2-NBDG astrocytes from 2-NBDG astrocytes. The expression levels of glycogen metabolic enzymes were analyzed in 2-NBDG and 2-NBDG astrocytes. We found unique glycogen metabolic patterns between 2-NBDG and 2-NBDG astrocytes. We also observed that 2-NBDG astrocytes were mainly identified as fibrous astrocytes but not protoplasmic astrocytes. Our data reveal cell type-dependent glycogen distribution and metabolism patterns, suggesting diverse functions of these different astrocytes.
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http://dx.doi.org/10.1007/s10571-019-00775-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261284PMC
July 2020

Facile Fabrication of Room-Temperature Self-Healing, Mechanically Robust, Highly Stretchable, and Tough Polymers Using Dual Dynamic Cross-Linked Polymer Complexes.

ACS Appl Mater Interfaces 2019 Sep 26;11(36):33356-33363. Epub 2019 Aug 26.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry , Jilin University , Changchun 130012 , P. R. China.

The development of polymeric materials with a combination of excellent mechanical performance and room-temperature self-healing property is still a huge challenge. Here, we report a facile method for the fabrication of dual dynamic cross-linked polymer complexes that simultaneously possess multiple remarkable mechanical properties and room-temperature self-healability by simply mixing polymers that have complementary interactions in solutions. Thanks to the synergistic effects of electrostatic and hydrogen-bonding interactions within their networks, the complexes obtained a superhigh tensile strength of 27.4 MPa and toughness of 110.0 MJ/m when compared with other polymers that can self-heal at room temperature. More importantly, the complexes can repair a physical cut in an ∼90% relative humid environment at room temperature with a high healing efficiency of ∼96% because of the dynamic nature of the noncovalent interactions. This method is a simple, low-cost, and widely applicable strategy for the large-scale fabrication of room-temperature self-healing materials that possess superior and controllable mechanical performances.
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http://dx.doi.org/10.1021/acsami.9b11166DOI Listing
September 2019

Two-Level Attention Network With Multi-Grain Ranking Loss for Vehicle Re-Identification.

IEEE Trans Image Process 2019 Sep 16;28(9):4328-4338. Epub 2019 Apr 16.

Vehicle re-identification (re-ID) aims to identify the same vehicle across multiple non-overlapping cameras, which is rather a challenging task. On the one hand, subtle changes in viewpoint and illumination condition can make the same vehicle look much different. On the other hand, different vehicles, even different vehicle models, may look quite similar. In this paper, we propose a novel Two-level Attention network supervised by a Multi-grain Ranking loss (TAMR) to learn an efficient feature embedding for the vehicle re-ID task. The two-level attention network consisting of hard part-level attention and soft pixel-level attention can adaptively extract discriminative features from the visual appearance of vehicles. The former one is designed to localize the salient vehicle parts, such as windscreen and car head. The latter one gives an additional attention refinement at pixel level to focus on the distinctive characteristics within each part. In addition, we present a multi-grain ranking loss to further enhance the discriminative ability of learned features. We creatively take the multi-grain relationship between vehicles into consideration. Thus, not only the discrimination between different vehicles but also the distinction between different vehicle models is constrained. Finally, the proposed network can learn a feature space, where both intra-class compactness and inter-class discrimination are well guaranteed. Extensive experiments demonstrate the effectiveness of our approach and we achieve state-of-the-art results on two challenging datasets, including VehicleID and Vehicle-1M.
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http://dx.doi.org/10.1109/TIP.2019.2910408DOI Listing
September 2019

Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis.

Evid Based Complement Alternat Med 2019 7;2019:7493286. Epub 2019 Feb 7.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, Shaanxi, China.

The concept of "acupoint sensitization" refers to the functional status of acupoint switches from silent to active under pathological conditions. In clinic, acupoint sensitization provides important guidance for acupoints selection in different diseases. However, the mechanism behind this phenomenon remains unclear. We generated a model of knee osteoarthritis (KOA) by intra-articular injection of monosodium iodoacetate (MIA) into the left knee of rats. The paw withdrawal mechanical threshold (PWMT) and the total number of mast cells as well as mast cell degranulation rate (MCDR) of acupoint tissue were used to test whether the acupoints were sensitized. The results showed that KOA resulted in a reduced mechanical threshold and elevated total number of mast cell as well as mast cell degranulation rate at bilateral ST35 (Dubi) but not GB37 (Guangming) or nonacupoint area. The acupoint sensitization was accompanied by upregulation of glycine transporter 2 (GlyT2) and reduction of extracellular glycine levels in the bilateral dorsal horns of the spinal cord at L3-5. Selective inhibition of GlyT2 or intrathecal administration of glycine attenuated ST35 acupoint sensitization. The sensitization of bilateral ST35 was blocked after intraspinal GlyT2 short hairpin (sh) RNA (GlyT2-shRNA) microinjection to specifically downregulate GlyT2 expression in the left side (ipsilateral) L3-5 spinal cord dorsal horn before MIA injection. Moreover, electroacupuncture (EA) stimulation at ST35 ameliorated articular pathological lesions and improved KOA-related pain behaviors. GlyT2-shRNA injection reversed EA-induced pain relief but not EA-induced reduction of joint lesions. Overall, this study demonstrated that spinal GlyT2, especially elevated GlyT2 expression in the ipsilateral dorsal horn of the spinal cord, is a crucial mediator of ST35 acupoint sensitization in KOA rats.
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http://dx.doi.org/10.1155/2019/7493286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383421PMC
February 2019

Acupoint Sensitization is Associated with Increased Excitability and Hyperpolarization-Activated Current (I) in C- But Not Aδ-Type Neurons.

Neuroscience 2019 04 1;404:499-509. Epub 2019 Mar 1.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, Shaanxi, China. Electronic address:

Under pathological conditions, acupoint sensitization is the phenomenon of acupoints transforming from the stable state to the dynamic state. Evidences suggest that hyperpolarization-activated current (I), conducted by the hyperpolarization-activated/cyclic nucleotide-gated (HCN) channel, greatly contributes to the peripheral and central sensitization. However, the role of the I current in acupoint sensitization has not been explained. In the present study, changes in excitability, I density and the HCN channel of dorsal root ganglion (DRG) nociceptive neurons were examined in the later phase of knee osteoarthritis (KOA) rats. To investigate the neuronal specificity of acupoint sensitization, retrograde dyes were injected into the acupoints ST35 and GB37. The results showed that acupoint sensitization occurred in bilateral ST35 but not GB37 acupoints. The excitability and I density of C- but not A-type neurons innervating ST35 acupoint increased in bilateral L5 DRG of acupoint sensitized rats than that of sham rats. No obvious changes were found in the excitability or I density of C- and A-type neurons innervating the GB37 acupoint in the bilateral L5 DRG. HCN channel subtype 2 (HCN2) expression levels significantly increased after acupoint sensitization. Furthermore, ZD7288, an HCN current (I) blocker, attenuated the acupoint sensitization of the ST35 acupoint. Taken together, our findings suggest that the increased excitability of C- but not A-type neurons and the upregulation of I/HCN2 channels contribute to the formation of acupoint sensitization.
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http://dx.doi.org/10.1016/j.neuroscience.2019.02.028DOI Listing
April 2019

Attention CoupleNet: Fully Convolutional Attention Coupling Network for Object Detection.

IEEE Trans Image Process 2019 Jan 13;28(1):113-126. Epub 2018 Aug 13.

The field of object detection has made great progress in recent years. Most of these improvements are derived from using a more sophisticated convolutional neural network. However, in the case of humans, the attention mechanism, global structure information, and local details of objects all play an important role for detecting an object. In this paper, we propose a novel fully convolutional network, named as Attention CoupleNet, to incorporate the attention-related information and global and local information of objects to improve the detection performance. Specifically, we first design a cascade attention structure to perceive the global scene of the image and generate class-agnostic attention maps. Then the attention maps are encoded into the network to acquire object-aware features. Next, we propose a unique fully convolutional coupling structure to couple global structure and local parts of the object to further formulate a discriminative feature representation. To fully explore the global and local properties, we also design different coupling strategies and normalization ways to make full use of the complementary advantages between the global and local information. Extensive experiments demonstrate the effectiveness of our approach. We achieve state-of-the-art results on all three challenging data sets, i.e., a mAP of 85.7% on VOC07, 84.3% on VOC12, and 35.4% on COCO. Codes are publicly available at https://github.com/tshizys/CoupleNet.
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http://dx.doi.org/10.1109/TIP.2018.2865280DOI Listing
January 2019

The novel estrogenic receptor GPR30 alleviates ischemic injury by inhibiting TLR4-mediated microglial inflammation.

J Neuroinflammation 2018 Jul 12;15(1):206. Epub 2018 Jul 12.

Department of Anaesthesiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710032, China.

Background: The steroid hormone estrogen (17-β-estradiol, E2) provides neuroprotection against cerebral ischemic injury by activating estrogen receptors. The novel estrogen receptor G protein-coupled receptor 30 (GPR30) is highly expressed in the brain and provides acute neuroprotection against stroke. However, the underlying mechanisms remain unclear.

Methods: In this study, ovariectomized female mice were subjected to middle cerebral artery occlusion (MCAO), and E2, G1, and ICI182780 were administered immediately upon reperfusion. The infarction volume, neurological scores, and neuronal injuries were examined. Primary microglial cells were subjected to oxygen-glucose deprivation (OGD), and the drugs were administered immediately upon reintroduction. The pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 in penumbra and microglia were assessed by ELISA. The cell viability and lactose dehydrogenase (LDH) release of neurons co-cultured with microglia were analyzed using cell counting kit-8 (CCK8) and LDH release assays. Microglial activation as well as GPR30, Iba1, and Toll-like receptor 4 (TLR4) protein expression and TLR4 mRNA expression were detected. Additionally, NF-κB activity was detected in lipopolysaccharide (LPS)-activated microglia after the activation of GPR30.

Results: GPR30 was highly expressed in microglia and significantly increased after ischemic injury. The activation of GPR30 significantly reduced the infarction volume, improved the neurological deficit, and alleviated neuronal injuries. Moreover, GPR30 activation significantly reduced the release of TNF-α, IL-1β, and IL-6 from ischemic penumbra and microglia subjected to OGD and alleviated neuronal injury as assessed using the CCK8 and LDH assays. Finally, the activation of GPR30 relieved microglial activation, reduced Iba1 and TLR4 protein expression and TLR4 mRNA levels, and inhibited NF-κB activity.

Conclusions: Microglial GPR30 exerts acute neuroprotective effects by inhibiting TLR4-mediated microglial inflammation, which indicates that GPR30 may be a potential target for the treatment of ischemic stroke.
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http://dx.doi.org/10.1186/s12974-018-1246-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6043971PMC
July 2018

A rat model of knee osteoarthritis suitable for electroacupuncture study.

Exp Anim 2018 May 1;67(2):271-280. Epub 2018 Jan 1.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the Fourth Military Medical University. 127th West Changle Road, Xi'an, 710032, Shaanxi, P.R. China.

Acupuncture is widely used for knee osteoarthritis (KOA) treatment in clinical practice. In the present study, we aimed to set a standard KOA animal model for electroacupuncture (EA) study and provide an acupuncture recipe for further KOA studies. Rats intra-articularly administered monosodium iodoacetate (MIA, 0.3, 1 or 3 mg respectively, n=12 each) were evaluated for pain-like behavior: paw withdrawal mechanical threshold, weight bearing deficit, and joint pathological changes (OARSI score) until 28 days after injury. Then by using the suitable dose (1 mg MIA), therapeutic effects of EA treatment (bilateral ST36 and ST35 acupoints, 2/10 Hz, 30 min/d, 6d/w, 2w) were evaluated in 3 groups (n=16 each): Early-on EA, Mid-term EA and Delayed EA, in which EA was started on day 1, day 7 or day 14 after MIA injection. Both 1 mg and 3 mg MIA induced significant joint damage and persistent pain behavior. But animals accepted 3 mg MIA rapidly developed cartilage and bone damage within 14 days. Early-on EA treatment provided significant pain relief and joint structure preservation in KOA rats. Mid-term EA treatment only reduced pain, while delayed EA treatment resulted in no effects in both aspects. 1 mg of MIA produces steady pain behavior and progressive joint damage, which was suitable for EA treatment evaluation. Early-on EA treatment provided both joint protection and pain reduction, while Mid-term EA could only be used for studying EA-induced analgesia in KOA.
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http://dx.doi.org/10.1538/expanim.17-0142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955758PMC
May 2018

TREK-1 pathway mediates isoflurane-induced memory impairment in middle-aged mice.

Neurobiol Learn Mem 2017 Nov 16;145:199-204. Epub 2017 Oct 16.

Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. Electronic address:

Post-operative cognitive dysfunction (POCD) has been widely reported, especially in elderly patients. An association between POCD and inhalational anesthetics, such as isoflurane, has been suggested. The TWIK-related K channel-1 (TREK-1) controls several major cellular responses involved in memory formation and is believed to participate in the development of depression, cerebral ischemia and blood-brain barrier dysfunction. However, the specific role of TREK-1 in mediating anesthesia-induced POCD remains unknown. In the current study, we determined that exposure to isoflurane affected memory in middle-aged mice and altered TREK-1 expression. In addition, TREK-1 over-expression exacerbated isoflurane-induced memory impairment, while TREK-1 silence attenuated the impairment. Taken together, our data demonstrate that inhibition of TREK-1 protects mice from cognitive impairment induced by anesthesia and TREK-1 is a potential therapeutic target against memory impairment induced by volatile anesthetics.
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http://dx.doi.org/10.1016/j.nlm.2017.10.012DOI Listing
November 2017

TREK-1 mediates isoflurane-induced cytotoxicity in astrocytes.

BMC Anesthesiol 2017 Sep 5;17(1):124. Epub 2017 Sep 5.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.

Background: There are growing concerns that anaesthetic exposure can cause extensive apoptotic degeneration of neurons and the impairment of normal synaptic development and remodelling. However, little attention has been paid to exploring the possible cytotoxicity of inhalation anaesthetics, such as isoflurane, in astrocytes. In this research, we determined that prolonged exposure to an inhalation anaesthetic caused cytotoxicity in astrocytes, and we identified the underlying molecular mechanism responsible for this process.

Methods: Astrocytes were exposed to isoflurane, and astrocytic survival was then measured via LDH release assays, MTT assays, and TUNEL staining. TWIK-related potassium (K) channel-1 (TREK-1) over-expression and knockdown models were also created using lentiviruses. The levels of TREK-1 and brain-derived neurotrophic factor (BDNF) were measured via Western blot and qRT-PCR.

Results: Prolonged exposure to isoflurane decreased primary astrocytic viability in a dose- and time-dependent manner. Moreover, with prolonged exposure to isoflurane, the TREK-1 level increased, and the BDNF level was reduced. TREK-1 knockdown promoted the survival of astrocytes and increased BDNF expression following isoflurane exposure.

Conclusions: Overdoses of and prolonged exposure to isoflurane induce cytotoxicity in primary astrocytes. TREK-1 plays an important role in isoflurane-induced cultured astrocytic cytotoxicity by down-regulating the expression of BDNF.
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http://dx.doi.org/10.1186/s12871-017-0420-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584525PMC
September 2017

Multi-View 3D Object Retrieval With Deep Embedding Network.

IEEE Trans Image Process 2016 12 15;25(12):5526-5537. Epub 2016 Sep 15.

In multi-view 3D object retrieval, each object is characterized by a group of 2D images captured from different views. Rather than using hand-crafted features, in this paper, we take advantage of the strong discriminative power of convolutional neural network to learn an effective 3D object representation tailored for this retrieval task. Specifically, we propose a deep embedding network jointly supervised by classification loss and triplet loss to map the high-dimensional image space into a low-dimensional feature space, where the Euclidean distance of features directly corresponds to the semantic similarity of images. By effectively reducing the intra-class variations while increasing the inter-class ones of the input images, the network guarantees that similar images are closer than dissimilar ones in the learned feature space. Besides, we investigate the effectiveness of deep features extracted from different layers of the embedding network extensively and find that an efficient 3D object representation should be a tradeoff between global semantic information and discriminative local characteristics. Then, with the set of deep features extracted from different views, we can generate a comprehensive description for each 3D object and formulate the multi-view 3D object retrieval as a set-to-set matching problem. Extensive experiments on SHREC'15 data set demonstrate the superiority of our proposed method over the previous state-of-the-art approaches with over 12% performance improvement.
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http://dx.doi.org/10.1109/TIP.2016.2609814DOI Listing
December 2016

Expression characteristics of , , and in families with a history of oesophageal cancer in a region with a high incidence of oesophageal cancer.

Oncol Lett 2015 Jan 7;9(1):430-436. Epub 2014 Nov 7.

Department of Thoracic Surgery, Linzhou Central Hospital, Linzhou, Henan 456550, P.R. China.

The aim of the present study was to determine the changes to the expression levels of fragile histidine triad (), breast cancer type 2 susceptibility protein (), MutL homolog 1 () and tumour protein 53 () exhibited by families with a history of oesophageal cancer in a region that has a high incidence of oesophageal cancer, and to determine the association of these changes with the cancer history of the families. Immunohistochemistry was used to detect the protein expression of FHIT, p53, BRCA2, and MLH1 in the excised specimens of cancer tissues from 74 oesophageal cancer patients (positive family history of oesophageal cancer [OCFH +], n=33; negative family history of oesophageal cancer [OCFH -], n=41) from a region with a high incidence of oesophageal cancer. The positive expression rates of (61%; 45/74), 50%; 37/74) and (27%; 9/33 in the oesophageal cancer tissues were significantly lower than those in the healthy tissues adjacent to the cancer (97% [29/30], 87% [26/30] and 73% [25/41], respectively). A significant difference was identified between the positive expression rates (P<0.01). However, , , and expression demonstrated no significant affect on clinicopathological changes, such as oesophageal cancerous tissue differentiation, the degree of infiltration and cancer cell metastasis. The , and expression levels were identified to be significantly lower in the cancer tissues from OCFH + patients. This result indicates that the expression levels of FHIT, BRCA2, and MLH1 are important molecular indices of genetic susceptibility to oesophageal cancer.
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http://dx.doi.org/10.3892/ol.2014.2682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246613PMC
January 2015