Publications by authors named "Haiyu Shao"

23 Publications

  • Page 1 of 1

Bibliometric Analysis of the Scientific Literature on Adolescent Idiopathic Scoliosis.

World Neurosurg 2021 Apr 16. Epub 2021 Apr 16.

Daniel and Jane Och Spine Hospital, New York-Presbyterian Healthcare System, Columbia University Medical Center, New York, NY, USA.

Background: In recent years, there has been increasing research on adolescent idiopathic scoliosis (AIS), leading to a large number of publications on this topic. To our knowledge, there is no study focused on the research trend in this field yet.

Methods: Using the Web of Science Core Collection database for all publications on AIS. The number of citations, authorship, year of publication, journal of publication, country and institution of origin and keywords were subjected to co-occurrence analysis using VOSviewer software. The top 100 most-cited publications on AIS were analyzed.

Results: Between 1985 and 2020, a total of 2266 publications related to AIS were identified. The frequency of publication on AIS has increased substantially over time. Among all countries, United States has contributed the most publications on AIS (n=671). The most productive institution has been Nanjing university (n=154). SPINE topped the list of journals and has published 569 AIS-related publications that received 19,862 total citations. The clinical description of AIS has been the most common research focus in the AIS literature.

Conclusion: The scientific literature on AIS has rapidly expanded in recent years. This study represents the updated bibliometric analysis of scientific publications on AIS and provides research trend for the first time, which aiming to give us a unique insight into the development of AIS research focus and serve as a useful guide to clinicians and researchers in the field.
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http://dx.doi.org/10.1016/j.wneu.2021.04.020DOI Listing
April 2021

Trelagliptin stimulates osteoblastic differentiation by increasing runt-related transcription factor 2 (RUNX2): a therapeutic implication in osteoporosis.

Bioengineered 2021 Dec;12(1):960-968

Department of Orthopedics, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China.

Osteoporosis, an aging-associated bone metabolic disease, is affecting millions of people worldwide. The deregulated process of osteoblastic differentiation has been linked with the progression of osteoporosis. Trelagliptin is a long-acting inhibitor of DPP-4 used for the management of type 2 diabetes mellitus. However, it is unknown whether Trelagliptin possesses a beneficial effect in osteoblastic differentiation. Interestingly, we found that treatment with Trelagliptin enhanced differentiation and promoted the mineralization of MC3T3-E1 cells. Firstly, Trelagliptin increased the activity of alkaline phosphatase (ALP) and promoted osteoblastic calcium deposition. Additionally, treatment with Trelagliptin upregulated ALP, osteocalcin (OCN), osteopontin (OPN), and bone morphogenetic protein-2 (BMP-2). Notably, Trelagliptin increased RUNX2, a major regulator of osteoblastic differentiation. Mechanistically, Trelagliptin upregulated the levels of p-AMPKα. Blockage of AMPK with compound C abolished the effects of Trelagliptin in RUNX2 and osteoblastic differentiation, suggesting the involvement of AMPK. Our findings suggest that Trelagliptin might possess a potential for the treatment of osteoporosis.
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http://dx.doi.org/10.1080/21655979.2021.1900633DOI Listing
December 2021

Bibliometric and Visualized Analysis of Scientific Publications on Ossification of the Posterior Longitudinal Ligament Based on Web of Science.

World Neurosurg 2021 May 19;149:e231-e243. Epub 2021 Feb 19.

Department of Orthopedic Surgery, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Background: In recent years, there has been increasing study of ossification of the posterior longitudinal ligament (OPLL), leading to many articles on this topic. We aimed to identify trends in OPLL-related research and to analyze the most highly cited scientific articles on OPLL.

Methods: We searched the Web of Science Core Collection database for all articles on OPLL. The years of publication, countries, journals, institutions, and total citations were extracted and analyzed. Results related to countries, institutions, and keywords were subjected to co-occurrence analysis using VOSviewer software. The top 100 most-cited articles on OPLL were analyzed.

Results: A total of 876 articles related to OPLL were identified. The frequency of publication on OPLL has increased substantially over time. Among all countries, Japan has contributed the most articles on OPLL (n = 349). The most productive institution has been Hirosaki University (n = 57). Spine topped the list of journals and has published 120 OPLL-related articles, which received 4221 total citations. The surgical treatment of OPLL has been the most common research focus in the OPLL literature.

Conclusions: The scientific literature on OPLL has rapidly expanded in recent years. This study represents the first bibliometric analysis of scientific articles on OPLL and can serve as a useful guide to clinicians and researchers in the field.
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http://dx.doi.org/10.1016/j.wneu.2021.02.045DOI Listing
May 2021

Top 100 Cited Articles on Spinal Disc Arthroplasty Research.

Spine (Phila Pa 1976) 2020 Nov;45(21):1530-1536

Daniel and Jane Och Spine Hospital, New York-Presbyterian Healthcare System, Columbia University Medical Center, New York, NY.

Study Design: A bibliometric review of current literature.

Objective: The purpose of this study was to identify and analyze the 100 most cited articles in spinal disc arthroplasty (SDA) research.

Summary Of Background Data: In the last several decades, SDA has been widely performed all over the world, with increasing popularity of cervical disc arthroplasty (CDA). While there is a large number of articles on this topic, to our knowledge, there is no bibliometric analysis yet.

Methods: All databases from the Web of Science were searched in a three-step approach. The information of the 100 most cited studies was collected, including title, first and last author, year of publication, journal, total citations, geographic origin, subspecialty, and types of artificial intervertebral disc for further analysis.

Results: The 100 most-cited articles were published from 1966 to 2015 in 9 different journals and were cited from 66 to 346 times. A total of 11 countries contributed to the 100 articles and the United States topped the list, with 54 articles, followed by Germany and France, with 10 and nine articles, respectively. There were more studies in CDA (n = 53) than lumbar disc arthroplasty (n = 35). Most of the studies reported clinical and radiographic outcomes (n = 33). The most productive periods were from 2006 to 2010. The majority of publications were in Spine, which published 43 articles. In total, 12 authors published more than two articles on the list.

Conclusion: Of the top 100 most cited articles on SDA, cervical papers outnumbered lumbar articles and the United States had 55 articles, with no other countries having more than 10. Our paper can help readers determine which of the thousands of articles on this topic are the most impactful and important ones to be familiar with.

Level Of Evidence: 3.
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http://dx.doi.org/10.1097/BRS.0000000000003608DOI Listing
November 2020

In Reply to "The Top 100 Most-Cited Articles on Kyphoplasty and Vertebroplasty".

World Neurosurg 2020 07;139:688-689

Hangzhou Medical School People's Hospital, Hangzhou, Zhejiang, China; Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China. Electronic address:

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http://dx.doi.org/10.1016/j.wneu.2020.04.076DOI Listing
July 2020

The 100 Most-Cited Publications in Endoscopic Spine Surgery Research.

Global Spine J 2021 May 25;11(4):587-596. Epub 2020 Jun 25.

74678Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.

Study Design: A bibliometric review of the literature.

Objective: Our objective was to identify and analyze the 100 most-cited publications in the field of endoscopic spine surgery (ESS).

Methods: In order to determine the top cited 100 articles, a 3-step approach was employed. First, the 100 most-cited ESS studies were identified using the key phrase "endoscopic spine surgery." Then, 8 keywords were identified from the 100 studies of step 1 were used to conduct a second round searching in all databases of the Web of Science. Finally, when the results of the first and second steps were overlapped, duplicated studies were removed. The 100 top-cited articles were used for further analysis.

Results: The citation number of the top 100 most-cited articles ranged from 44 to 236 with a mean value of 84.4. The most productive periods were from 2001 to 2010. The majority of publications came from and , where holds the largest number of 35 articles, followed by with 13 articles. Overall, 10 countries contributed to the 100 articles, with the most productive country being the United States, followed by Germany and Korea.

Conclusion: This bibliometric study is meant to produce a list of intellectual milestones in the field of ESS. This article's identification of the most influential articles in the field of ESS gives us a unique and comprehensive insight into the development of ESS in the past several decades.
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http://dx.doi.org/10.1177/2192568220934740DOI Listing
May 2021

Minimally invasive surgery for low-grade spondylolisthesis: percutaneous endoscopic or oblique lumbar interbody fusion.

J Comp Eff Res 2020 06 18;9(9):639-650. Epub 2020 Jun 18.

Department of Orthopaedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang Province, China.

To compare the clinical and radiographic outcomes of percutaneous endoscopic-assisted lumbar interbody fusion (PELIF) versus oblique lumbar interbody fusion (OLIF) for the treatment of symptomatic low-grade lumbar spondylolisthesis. The clinical and radiographic records of 48 patients underwent single-level minimally invasive lumbar fusion with a PELIF (n = 16) or OLIF (n = 32) were reviewed. The clinical and radiographic outcomes were similar in both groups. PELIF procedure exhibited superior capability of the enlargement of foraminal width, but inferior capability of the restoration of foraminal height than OLIF procedure. PELIF minimizes the iatrogenic damages and perioperative risks to a great extent, and seems to be a promising option for the treatment of symptomatic low-grade lumbar spondylolisthesis.
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http://dx.doi.org/10.2217/cer-2020-0022DOI Listing
June 2020

Percutaneous endoscopic-assisted direct repair of pars defect without general anesthesia could be a satisfying treatment alternative for young patient with symptomatic lumbar spondylolysis: a technique note with case series.

BMC Musculoskelet Disord 2020 Jun 2;21(1):340. Epub 2020 Jun 2.

Department of Orthopaedics, Zhejiang Provincial People's Hospital, Shangtang Road No. 158, Hangzhou, 310014, Zhejiang Province, China.

Background: Multiple surgical procedures are applied in young patients with symptomatic lumbar spondylolysis when conservative treatments fail. Although the optimal surgical procedure option is controversial, the treatment paradigm has shifted from open surgery to minimally invasive spine surgery. To date, a limited number of studies on the feasibility of percutaneous endoscopic-assisted direct repair of pars defect have been carried out. Herein, for the first time, we retrospectively explore the outcomes of pars defect via percutaneous endoscopy.

Methods: We retrospectively examined young patients with spondylolysis treated using the percutaneous endoscopic-assisted direct repair of pars defect supplemented with autograft as well as percutaneous pedicle screw fixation between September 2014 and December 2018. Six patients with a mean age of 18.8 years were enrolled in the study. We used preoperatively computed tomographic (CT) scans to evaluate the size of pars defect, and graded disc degeneration using Pfirrmann's classification through magnetic resonance images (MRI). We assessed the clinical outcomes using the Oswestry Disability Index (ODI), 36-Item Short-Form Health Survey (SF-36) as well as Visual Analogue Scale for back pain (VAS-B).

Results: Our findings revealed that pain intensity and function outcomes, including VAS-B, ODI, and SF-36 (PCS and MCS) scores, were markedly improved after surgery and at the final follow-up visit. The change in the gap distance of the pars defect was remarkably significant after surgery and during the follow-up period. Only one of the 12 pars repaired was reported as a non-union at the final follow-up visit. Moreover, no surgery-related complications were reported in any of the cases.

Conclusion: Percutaneous endoscopic-assisted direct repair of pars defect without general anesthesia, a minimally invasive treatment option, supplemented with autograft and percutaneous pedicle screw fixation, could be a satisfying treatment alternative for young patients with symptomatic lumbar spondylolysis.
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http://dx.doi.org/10.1186/s12891-020-03365-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268338PMC
June 2020

Percutaneous transforaminal endoscopic decompression for the treatment of intraspinal tophaceous gout: A case report.

Medicine (Baltimore) 2020 May;99(21):e20125

Department of Orthopedics, Zhejiang Provincial People's Hospital.

Rationale: Intraspinal tophaceous gout is relatively rare condition presenting with major clinical manifestations, such as spinal cord or nerve roots compressions (radiculopathy). It is usually difficult to differentiate intraspinal tophaceous gout, lumbar disc herniation, stenosis of spinal canal, ossification of ligamentum flavum, and other degenerative spinal disorders from each other.

Patient Concerns: A 64-year-old man was admitted with a history of progressive low back pain for 11 months. He also presented with radiculopathy and numbness of his left lower extremity.

Diagnoses: Preoperative computed tomography (CT) and magnetic resonance imaging (MRI) showed L4/5 disc herniation and lateral recess stenosis on the left side. During the operation of percutaneous transforaminal endoscopic decompression, intraspinal chalky white material was seen. Post-operative pathologic results confirmed the diagnosis of gouty tophi.

Interventions: Percutaneous transforaminal endoscopic decompression was performed as treatment. Intraspinal chalky white material was seen. We removed most of the chalky white material and extruded nucleus.

Outcomes: His symptom subsided rapidly and no deterioration was noted 1 year post-operatively.

Lessons: Although intraspinal tophaceous gout is not commonly seen, clinicians should take it into consideration as a possible differential diagnosis when the patient exhibits axial pain or neurological deficits with risk factors of gout. We identified and treated this case with percutaneous transforaminal endoscopic decompression for the first time and got an excellent outcome. Percutaneous transforaminal endoscopic surgery proved to be an effective and minimally invasive alternative for identifying and treating intraspinal tophaceous gout.
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http://dx.doi.org/10.1097/MD.0000000000020125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249926PMC
May 2020

Novel prognostic nomograms for female patients with breast cancer and bone metastasis at presentation.

Ann Transl Med 2020 Mar;8(5):197

Department of Orthopaedics, Centre for Orthopaedic Research, Orthopedics Research Institute of Zhejiang University, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310000, China.

Background: There is a paucity of literature about prognostic evaluation for patients with breast cancer (BC) and bone metastasis at presentation. To date, little is known about how to accurately predict the prognosis of BC patients with bone metastasis at presentation. Thus, an accurate prediction tool of prognosis in this population is urgently needed. Our goal is to construct novel and prognostic nomograms for BC patients with bone metastasis at presentation.

Methods: We searched Surveillance, Epidemiology, and End Results (SEER) database for BC patients with bone metastasis at presentation between 2010 and 2016. Multivariate analysis was performed to obtain significantly independent variables. Then, novel prognostic nomograms were constructed based on those independent predictors.

Results: Tumor grade, histological type, primary tumor size, tumor subtype, surgery, chemotherapy and number of metastatic organs except bone were recognized as significantly independent variables of both overall survival (OS) and cancer-specific survival (CSS). Then those significant variables were integrated to construct nomograms for 3- and 5-year survival. Calibration plots for the 3- and 5-year survival in training and validation sets showed that the prediction curve was close to a 45 degree slash. The C-indices of OS in training and validation cohorts were 0.705 and 0.678, respectively. Similar results were observed for CSS in training and validation cohorts.

Conclusions: Our proposed nomograms can effectively and accurately predict the prognosis of BC patients with bone metastasis at presentation, which provide a basis for individual treatments for metastatic lesions.
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http://dx.doi.org/10.21037/atm.2020.01.37DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154431PMC
March 2020

Percutaneous Transforaminal Endoscopic Lumbar Interbody Fusion for Degenerative Lumbar Diseases: A Consecutive Case Series with Mean 2-Year Follow-Up.

Pain Physician 2020 03;23(2):165-174

Department of Orthopaedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang Province, China; People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang Province, China; Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, China.

Background: Conventional open surgical procedures may cause massive dissections of the spine, higher perioperative complications, prolonged hospitalization, protracted rehabilitation programs and recovery. Percutaneous endoscopic lumbar interbody fusion (PELIF) is an evolving treatment option.

Objectives: To present the detailed procedure and preliminary clinical and radiologic results of PELIF for degenerative lumbar diseases.

Study Design: A retrospective cohort study.

Setting: A university affiliated tertiary hospital.

Methods: The medical records of patients with degenerative lumbar diseases who underwent PELIF between January 2016 and December 2017 were retrospectively reviewed. Surgical level, surgical time, blood loss, hospital length of stay, and perioperative complications were discussed. Patients were also evaluated for pain by the Visual Analog Scale (VAS), and functional assessment by the Oswestry Disability Index (ODI) and the 36-Item Short Form Health Survey (SF-36), including Physical Component Summary (PCS) and Mental Component Summary (MCS) preoperatively, postoperatively, and during the follow-up period.

Results: Thirty-nine consecutive patients (25 men and 14 women) with a mean age of 59.0 years (range, 39-77 years) were enrolled. The average surgical time was 213.8 ± 31.7 minutes (range, 185-324 minutes). Mean estimated blood loss was 25.0 ± 12.6 mL (range, 15-50 mL). At the latest follow-up visit, the VAS scores for back pain, leg pain, ODI, and SF-36 (MCS/PCS) scores improved 89.5%, 95.0%, 71.2%, and 37.5%/58%, respectively. Reoperations were performed in one patient for residual disc mass and one for misplacement of pedicle screw. Fusion was achieved in all patients.

Limitations: The presented results are preliminary and should be interpreted taking the limitations into account, including nonrandomized design, relatively small sample size, and less intensive follow-up period.

Conclusions: The presented PELIF technique seems to be a promising surgical alternative for the treatment of patients with specific degenerative lumbar diseases. Randomized studies with larger sample size and long-term follow-up duration are needed to validate the superiorities of this versatile surgery.

Key Words: Endoscopic, minimally invasive spine surgery, lumbar interbody fusion, disc herniation, spondylolisthesis.
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March 2020

The Top 100 Most-Cited Articles on Kyphoplasty and Vertebroplasty.

World Neurosurg 2020 Mar 11;135:e435-e446. Epub 2019 Dec 11.

Department of Spine Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China; Hangzhou Medical College People's Hospital, Hangzhou, Zhejiang, China. Electronic address:

Introduction: Over the last several decades, both percutaneous kyphoplasty (PKP) and percutaneous vertebroplasty (PVP) have been used for pain relief in patients with osteoporotic vertebral compression fractures. The purpose of our study was to use citation analysis to identify and review the top 100 most-cited publications regarding PKP and PVP.

Methods: All databases of the Web of Science were searched using the keywords "kyphoplasty" and "vertebroplasty." All publications with >100 citations were identified and the results were ranked in descending order of citations. The 100 most-cited publications were included for analysis.

Results: A total of 6271 publications on PKP and PVP were identified. The number of citations of the 100 most-cited studies ranged from 735 to 109, with a mean of 225.3 citations per study. The most productive period was 2001-2010, which produced 79 of the top 100 publications. Thirteen journals published these 100 studies, with Spine publishing the largest number (23) of studies. Most of the identified articles originated in the United States, with France and Switzerland found to be the next most heavily represented countries of origin of the 11 countries that produced them. Most of the studies focused on treatment of osteoporotic vertebral compression fractures, followed by pathologic fractures caused by tumors.

Conclusions: We identified the 100 most-cited publications on PKP and PVP and performed a bibliometric analysis characterizing distinguishing features of these studies. This list can help guide clinical decision making and future research directions as clinicians and researchers continue to explore these controversial therapeutic techniques.
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http://dx.doi.org/10.1016/j.wneu.2019.12.014DOI Listing
March 2020

Cauda Equina Syndrome as First Manifestation of von Hippel-Lindau Disease.

World Neurosurg 2019 05 16;125:316-319. Epub 2019 Feb 16.

Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, China; Hangzhou Medical College People's Hospital, Hangzhou, China. Electronic address:

Background: Von-Hippel Lindau (VHL) disease is rare neurocutaneous disorder arising from an inactivating mutation of the VHL gene on chromosome 3p35. The disease is inherited in an autosomal dominant fashion and predominantly involves tissues originating from the ectodermal germ layer. Although hemangioblastomas of retina and cerebellum are the most common clinical features, multiple-organ involvement in the form of renal cell carcinomas, spinal hemangioblastomas, renal cysts, and pancreatic cysts have also been reported. Hemangioblastomas of the spinal canal could present as cauda equina syndrome.

Case Description: We describe the case of a 55-year-old man with cauda equina syndrome as his first manifestation. An initial magnetic resonance imaging scan uncovered hemangiomas in the spinal canal. A thorough family history strongly suggested an autosomal dominant pattern of disease inheritance. Further physical examination and imaging revealed additional organ involvement consistent with VHL. We performed laminectomy and partial resection of hemangioblastoma for decompression. He recovered satisfactorily with his cauda equina syndrome resolving quickly post operation. No deterioration was apparent by the 12-month follow-up visit.

Conclusions: To the best of our knowledge, this is the first case with cauda equina syndrome as the first manifestation of VHL disease. Laminectomy and partial resection of hemangioblastoma are safe and effective treatments to resolve impingement of the spinal cord. VHL disease resulting in hemangioblastoma in the spinal canal is rarely seen but should be included in the differential diagnosis of cauda equina syndrome to facilitate genetic counseling for the proband and offspring.
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http://dx.doi.org/10.1016/j.wneu.2019.01.269DOI Listing
May 2019

miR-33a Mediates the Anti-Tumor Effect of Lovastatin in Osteosarcoma by Targeting CYR61.

Cell Physiol Biochem 2018 22;51(2):938-948. Epub 2018 Nov 22.

People's Hospital of Hangzhou Medical College, Hangzhou,

Background/aims: Preventing cell metastasis is an effective therapeutic strategy to treat osteosarcoma and improve prognosis. Statins have been found to have anticancer effects in addition to their cholesterol-lowering action. As a new target of statins, cysteine-rich 61 (CYR61) was recently identified to promote cell migration and metastasis in osteosarcoma. However, the underlying mechanisms mediating the regulation of CYR61 expression by statins remain unknown.

Methods: Human osteosarcoma cell lines MG63 and SaOS2 were used to clarify the effect of lovastatin on CYR61 expression. Real-time PCR was performed to detect mRNA or microRNA (miRNA) levels and western blot was performed to detect protein levels. Cell invasive ability was determined using Transwell assays. Lentivirus encoding CYR61 cDNA or sterol regulatory element-binding protein 2 (SREBP-2) shRNA was used to upregulate CYR61 expression or downregulate SREBP-2 expression. Binding of the CYR61 3' untranslated region (UTR) and miR-33a was analyzed by luciferase reporter assay.

Results: We found that lovastatin treatment decreased CYR61 expression, inhibited cell invasion and altered epithelial-to-mesenchymal-transition (EMT)-related protein expression, while CYR61 overexpression abolished the effect of lovastatin. Moreover, lovastatin increased the expression of SREBP-2 and miR-33a, which were then downregulated by SREBP-2 silencing. Bioinformatics analysis indicated that the CYR61 3'UTR harbored a potential miR-33a binding site and luciferase reporter assay demonstrated that CYR61 was a target of miR-33a in osteosarcoma cells. Furthermore, miR-33a could inhibit cell invasion and alter EMT-related protein expression. SREBP-2 silencing or miR-33a inhibitor upregulated CYR61 expression and reversed the effects of lovastatin on cell invasion and EMT-related proteins.

Conclusion: Our findings suggest lovastatin suppresses osteosarcoma cell invasion through the SREBP-2/miR-33a/CYR61 pathway.
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http://dx.doi.org/10.1159/000495396DOI Listing
December 2018

miRNA-340 inhibits osteoclast differentiation via repression of MITF.

Biosci Rep 2017 08 20;37(4). Epub 2017 Jul 20.

People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China

Many miRNAs play critical roles in modulating various biological processes of osteoclast differentiation and function. Microphthalmia-associated transcription factor (MITF), a target of miR-340, served as pivotal transcription factor involved in osteoclast differentiation. However, the role of miR-340 and MITF during osteoclast differentiation has not yet been clearly established. Tartrate-resistant acid phosphatase (TRAP) staining assay was performed to identify osteoclasts differentiated from bone marrow-derived macrophages (BMMs). Quantitative reverse transcription PCR (qRT-PCR) or Western blotting was undertaken to examine the mRNA or protein expression respectively. Luciferase reporter assay was performed to investigate the interaction between miR-340 and MITF. MITF was knocked down and miR-340 was overexpressed and transfected into BMMs to detect their effects on osteoclast differentiation. Firstly, qRT-PCR analysis showed that miR-340 was down-regulated during osteoclast differentiation stimulated by macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-κB (RANK) ligand (RANKL). Besides, we found that overexpression of miRNA-340 inhibited osteoclast differentiation and suppressed both the mRNA and protein level of MITF. Finally, Western blot and qRT-PCR analysis revealed that silencing MITF inhibited TRAP, calcitonin receptor, V-ATPase d2, and cathepsin K. miR-340 suppresses osteoclast differentiation by inhibiting MITF. Our findings may provide promising therapeutic targets for osteoclast-associated diseases.
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http://dx.doi.org/10.1042/BSR20170302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518531PMC
August 2017

Erratum to: Transforming Growth Factor b1/Smad4 Signaling Affects Osteoclast Differentiation via Regulation of miR-155 Expression.

Mol Cells 2017 05;40(5):378

Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.

[This corrects the article on p. 211 in vol. 40.].
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http://dx.doi.org/10.14348/molcells.2017.1303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5463047PMC
May 2017

Transforming Growth Factor β1/Smad4 Signaling Affects Osteoclast Differentiation via Regulation of miR-155 Expression.

Mol Cells 2017 Mar 29;40(3):211-221. Epub 2017 Mar 29.

Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.

Transforming growth factor β1 (TGFβ1)/Smad4 signaling plays a pivotal role in maintenance of the dynamic balance between bone formation and resorption. The microRNA miR-155 has been reported to exert a significant role in the differentiation of macrophage and dendritic cells. The goal of this study was to determine whether miR-155 regulates osteoclast differentiation through TGFβ1/Smad4 signaling. Here, we present that TGFβ1 elevated miR-155 levels during osteoclast differentiation through the stimulation of M-CSF and RANKL. Additionally, we found that silencing Smad4 attenuated the upregulation of miR-155 induced by TGFβ1. The results of luciferase reporter experiments and ChIP assays demonstrated that TGFβ1 promoted the binding of Smad4 to the miR-155 promoter at a site located in 454 bp from the transcription start site , further verifying that miR-155 is a transcriptional target of the TGFβ1/Smad4 pathway. Subsequently, TRAP staining and qRT-PCR analysis revealed that silencing Smad4 impaired the TGFβ1-mediated inhibition on osteoclast differentiation. Finally, we found that miR-155 may target SOCS1 and MITF to suppress osteoclast differentiation. Taken together, we provide the first evidence that TGFβ1/Smad4 signaling affects osteoclast differentiation by regulation of miR-155 expression and the use of miR-155 as a potential therapeutic target for osteoclast-related diseases shows great promise.
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http://dx.doi.org/10.14348/molcells.2017.2303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386959PMC
March 2017

Grape seed procyanidin B2 inhibits adipogenesis of 3T3-L1 cells by targeting peroxisome proliferator-activated receptor γ with miR-483-5p involved mechanism.

Biomed Pharmacother 2017 Feb 21;86:292-296. Epub 2016 Dec 21.

Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. Electronic address:

Procyanidins have lipolysis effect on adipose metabolism, but the underlying mechanism is not fully understood. The aim of present study was to examine the effect of grape seed procyanidin B2 (GSP) on the adipogenic differentiation of 3T3-L1 preadipocyte cell line and investigate the underlying mechanism. The results showed that GSP treatment significantly reduced the intracellular lipid accumulation in induced 3T3-L1 cells by targeting miR-483-5p as well as peroxisome proliferator-activated receptor γ (PPARγ). In addition, our results revealed that overexpression of miR-483-5p increased adipogenic differentiation, while inhibition of miR-483-5p reduced the lipid accumulation by suppressing the adipogenic differentiation. Moreover, overexpression of miR-483-5p could reverse GSP's inhibition of adipocyte differentiation as well as increase the level of PPARγ. These results demonstrate that GSP inhibits adipogenesis by targeting PPARγ and suggest this effect might be mediated by miR-483-5p.
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http://dx.doi.org/10.1016/j.biopha.2016.12.019DOI Listing
February 2017

Zero-profile implant versus conventional cage-plate implant in anterior cervical discectomy and fusion for the treatment of degenerative cervical spondylosis: a meta-analysis.

J Orthop Surg Res 2015 Sep 17;10:148. Epub 2015 Sep 17.

Department of Orthopaedics, Zhejiang Provincial People's Hospital, No.158, Shangtang Road, Hangzhou, 310000, Zhejiang, China.

Background: Zero-profile implant has become more and more popular in anterior cervical discectomy and fusion (ACDF) for the treatment of degenerative cervical spondylosis. However, there was no enough evidence judging its efficiency and safety. The aim of this analysis was to evaluate the efficacy and safety of Zero-profile implant compared with conventional cage-plate (CCP) in ACDF.

Methods: All studies directly comparing the outcomes between the Zero-profile implant and CCP implant in ACDF were included, and the search strategy followed the requirements of the Cochrane Library Handbook. Two of the authors extracted relevant data and checked the accuracy independently using standardized data collection form.

Results: Seven studies involving 560 patients were included, 262 in the Zero-profile group and 298 in the CCP group. Zero-profile implant had a lower rate of postoperative dysphagia at 2 weeks, 6 months, and 1 year (p = 0.0002, p = 0.008, and p = 0.001, respectively) than CCP implant. Zero-profile also reduced blood loss (p = 0.0001), while operation time and incidence of postoperative transient dysphagia had no statistical significance (p = 0.92, p = 0.42, respectively) between two groups.

Conclusion: Based on the results of our analysis, the application of Zero-profile implant in ACDF had a lower rate of postoperative dysphagia at 2 weeks, 6 months, and 1 year than CCP implant. Zero-profile implant also had fewer blood loss during operation. More rigorous and adequately powered prospective randomized controlled trials with larger sample size are required to elucidate a more objective outcome.
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http://dx.doi.org/10.1186/s13018-015-0290-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574194PMC
September 2015

Association between duration of playing video games and bone mineral density in Chinese adolescents.

J Clin Densitom 2015 Apr-Jun;18(2):198-202

Department of DEXA, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.

The aim of the study was to investigate the association between duration of playing video games and bone mineral density (BMD) in Chinese adolescents. Three hundred eighty-four Chinese adolescents aged 14-18 yr (148 males and 236 females) were analyzed. Anthropometric measurements were obtained using standard procedures. Total body and regional BMD were measured using dual-energy X-ray absorptiometry. Duration of playing video games, defined as hours per day, was measured by a self-report questionnaire. We examined the association between duration of playing video games and BMD using multiple linear regression analysis. After adjustment for age, sex, pubertal stage, parental education, body mass index, adolescents with longer video game duration were more likely to have lower legs, trunk, pelvic, spine, and total BMD (p < 0.05). We concluded that duration of video game was negatively associated with BMD in Chinese adolescents. These findings provide support for reducing duration of playing video games as a possible means to increase BMD in adolescents. Future research is needed to elucidate the underlined mechanisms linking playing video games and osteoporosis.
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http://dx.doi.org/10.1016/j.jocd.2015.02.007DOI Listing
August 2015

Associations of fat mass and fat distribution with bone mineral density in Chinese obese population.

J Clin Densitom 2015 Jan-Mar;18(1):44-9. Epub 2014 May 9.

Department of Dual Energy X-ray Absorptiometry (DEXA), Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China. Electronic address:

The purpose of the study was to investigate the associations of fat mass (FM) and fat distribution with bone mineral density (BMD) in Chinese obese population. Three hundred and forty-seven Chinese obese females and 339 males aged 20-39 years were analyzed. Lean mass (LM), FM, percent body fat (%BF), android FM, gynoid FM, and total and regional BMD were measured using dual-energy X-ray absorptiometry. Fat distribution was assessed by android-to-gynoid FM ratio (AOI). As a result, increased central body fat had an inverse association with total and leg BMD in females but not in males. Increased FM and %BF were positively associated with arm, trunk, and pelvic BMD in Chinese obese females. Increased FM was positively associated with total, rib, and trunk BMD in Chinese obese males. The results remained almost unchanged after adjusting for LM, and LM was significantly positively associated with spine BMD in female group. FM was positively associated with trunk BMD in male group after adjusting for LM. AOI was inversely associated with total and leg BMD, and %BF was positively associated with arm, trunk, and pelvic BMD when replacing FM with %BF in female group. The results remained almost unchanged after adjusting for LM. There is no significant association in male group when replacing FM with %BF. In conclusion, our findings demonstrate that there are different associations of FM and fat distribution with BMD, and AOI has a negative association with BMD.
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http://dx.doi.org/10.1016/j.jocd.2014.03.001DOI Listing
March 2015

Hemostatic techniques following multilevel posterior lumbar spine surgery: a randomized control trial.

J Spinal Disord Tech 2014 Dec;27(8):442-6

Spinal Unit of Orthopedic Department of Zhejiang Provincial People's Hospital, Zhejiang, P. R. China.

Study Design: This was a prospective, randomized controlled clinical study.

Objective: To determine the efficacy of absorbable gelatin sponge in reducing blood loss, as well as shortening the length of hospital stay in patients undergoing multilevel posterior lumbar spinal surgery.

Background: Absorbable gelatin sponge is reported to decrease postoperative drain output and the length of hospital stay after multilevel posterior cervical spine surgery. However, there is a dearth of literature on prospective study of the efficacy of absorbable gelatin sponge in reducing postoperative blood loss, as well as shortening the length of hospital stay in patients undergoing multilevel posterior lumbar spinal surgery.

Materials And Methods: A total of 82 consecutive patients who underwent multilevel posterior lumbar fusion or posterior lumbar interbody fusion between June 2011 and June 2012 were prospectively randomized into one of the 2 groups according to whether absorbable gelatin sponge for postoperative blood management was used or not. Demographic distribution, total drain output, blood transfusion rate, the length of stay, the number of readmissions, and postoperative complications were analyzed.

Results: Total drain output averaged 173 mL in the study group and 392 mL in the control group (P=0.000). Perioperative allogeneic blood transfusion rate were lower in the Gelfoam group (34.1% vs. 58.5%, P=0.046); moreover, length of stay in patients with the use of absorbable gelatin sponge (12.58 d) was significantly shorter (P=0.009) than the patients in the control group (14.46 d). No patient developed adverse reactions attributable to the absorbable gelatin sponge.

Conclusions: Application of absorbable gelatin sponge at the end of multilevel posterior lumbar fusion can significantly decrease postoperative drain output and length of hospital stay.
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http://dx.doi.org/10.1097/BSD.0000000000000063DOI Listing
December 2014

Nicotine should be judged without fear and favor.

Med Hypotheses 2010 Nov 6;75(5):471. Epub 2010 May 6.

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http://dx.doi.org/10.1016/j.mehy.2010.04.014DOI Listing
November 2010