Publications by authors named "Haiyang Chen"

101 Publications

Research progress of DLX6-AS1 in human cancers.

Hum Cell 2021 Sep 11. Epub 2021 Sep 11.

Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.

Long non-coding RNAs (lncRNAs) are a kind of translational-repressor RNAs composed of more than 200 nucleotides and formerly considered as "transcriptional noise". Recently studies have shown that lncRNAs could bind to multiple biomolecules such as DNA, transcription factors, RNA, chromatin complexes and proteins, and regulate target gene expression at multi-levels, thus playing an essential role in human tumors. DLX6-AS1, a recently discovered oncogenic lncRNA, is highly expressed in various human tumors, including lung cancer, liver cancer and pancreatic cancer. This paper mainly reviewed the regulatory mechanism of DLX6-AS1 as a competitive endogenous RNA (ceRNA) in tumor cell proliferation, cell apoptosis, angiogenesis, epithelial-mesenchymal transformation, chemotherapy resistance and metabolic changes. Furthermore, the translational value of DLX6-AS1 in cancer was also elucidated, which suggested its potential as a diagnostic or prognostic biomarker in cancer. In summary, this present article not only makes an in-depth analysis of the expression changes and carcinogenic mechanism of DLX6-AS1 in various human cancers, but also provides a new breakthrough for the diagnosis and treatment of cancers.
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http://dx.doi.org/10.1007/s13577-021-00613-0DOI Listing
September 2021

A quality evaluation of the clinical practice guidelines on breast cancer using the RIGHT checklist.

Ann Transl Med 2021 Jul;9(14):1174

Department of Internal Medicine, Henan Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, China.

Background: Breast cancer is the most frequent type of cancer in women. The methodological quality of clinical practice guidelines (CPGs) on breast cancer has been shown to be heterogeneous. The aim of our study was to evaluate the quality of breast cancer CPGs published in years 2018-2020, using the Reporting Items for Practice Guidelines in Healthcare (RIGHT) checklist.

Methods: We searched Medline (via PubMed), Chinese National Knowledge Infrastructure (CNKI), Wanfang and Chinese Biomedical Literature (CBM) as well as websites of guideline organizations for CPGs on breast cancer published between 2018 and 2020. We used the RIGHT checklist to evaluate the reporting quality of the included guidelines by assessing whether the CPGs adhered to each item of the checklist and calculated the proportions of appropriately reported RIGHT checklist items. We also presented the adherence reporting rates for each guideline and the mean rates for each of the seven domains of the RIGHT checklist.

Results: A total of 45 guidelines were included. Eighteen (40.0%) guidelines had an overall reporting rate below 50% and only three (6.7%) reported more than 80% of the items. The domains "Basic information" and "Background" had the highest reporting rates (75.9% and 62.5%, respectively). The mean reporting rates of the domains "Evidence", "Recommendation", "Review and quality assurance", "Funding and declaration and management of interests" and "Other information" were 42.7%, 53.0%, 33.3%, 45.0%, and 44.4%, respectively.

Conclusions: The reporting quality varied among guidelines for breast cancer, showing the need for improvement in reporting the contents. Guideline developers should pay more attention to reporting the evidence, review and quality assurance, and funding and declaration and management of interests in future.
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http://dx.doi.org/10.21037/atm-21-2884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350626PMC
July 2021

Curcumin induced G2/M cycle arrest in SK-N-SH neuroblastoma cells through the ROS-mediated p53 signaling pathway.

J Food Biochem 2021 Sep 31;45(9):e13888. Epub 2021 Jul 31.

Department of Pediatric Urology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

Neuroblastoma (NB) is a solid tumor in the nervous system and has a high mortality rate in children. Curcumin has well-characterized anticancer properties, while there is no effective method in clinical treatment. MTT assays revealed that curcumin dramatically inhibited the proliferation of SK-N-SH cells. Compared with the control group, curcumin markedly restrained the migration of SK-N-SH cells. Curcumin induced SK-N-SH cell apoptosis by G2/M cycle arrest and activated caspase-3 activity. Furthermore, curcumin promoted the overproduction of intracellular ROS and apoptosis induced by activating p53 and Bcl-2 signal pathways. This finding demonstrated the application of curcumin is an effective strategy for the therapeutics of NB.
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http://dx.doi.org/10.1111/jfbc.13888DOI Listing
September 2021

An evaluation of the reporting quality in clinical practice guidelines for hepatocellular carcinoma using the RIGHT checklist.

Ann Transl Med 2021 Jun;9(12):1004

Department of Internal Medicine, Henan Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, China.

Background: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. Clinical practice guidelines (CPGs) on the prevention, surveillance, diagnosis and management of HCC are essential to guide clinical practice. The objective of this study was to evaluate the reporting quality of the most recent CPGs for HCC published worldwide.

Methods: We systematically searched literature databases and websites of guideline development organizations and medical associations to extract CPGs on HCC published between January 2018 and December 2020. We evaluated the reporting quality using the Reporting Items for practice Guidelines in Healthcare (RIGHT) statement. We assessed for each of the 35 RIGHT checklist items whether the guidelines reported the corresponding information. We calculated the mean (± standard error of the mean, SEM) percentages of the guidelines' compliance with the items (reporting rate), both overall and for each of the seven domains of the RIGHT checklist.

Results: We identified 22 guidelines, of which three (14%) were written in Chinese and 19 (86%) in English. The mean ±SEM overall reporting rate in the twenty-two guidelines was 56%±4%. The reporting rates of the seven domains were the following: basic information 81%±3%, background 58%±6%, evidence 58%±6%, recommendations 59%±5%, review and quality assurance 34%±10%, funding and declaration and management of interests 39%±4%, and other information 23%±6%.

Conclusions: The reporting quality of the recently published guidelines for HCC was suboptimal. While there is no doubt about the great value of the CPGs' recommendations in clinical practice, the reporting in CPGs for HCC still needs improvement.
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http://dx.doi.org/10.21037/atm-21-2611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267298PMC
June 2021

Evaluation of the reporting quality of guidelines for gastric cancer using the RIGHT checklist.

Ann Transl Med 2021 Jun;9(12):1003

Department of Internal Medicine, Henan Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, China.

Background: Gastric cancer is the fifth most common type of cancer globally. We aimed to evaluate the reporting quality of clinical practice guidelines in the field of gastric cancer.

Methods: We searched Medline (via PubMed), China Biology Medicine, Chinese National Knowledge Infrastructure and WanFang databases and the websites of the main guideline development organizations from 2018 to 2020 for guidelines on gastric cancer. Data were extracted and the reporting quality evaluated by two researchers independently using the Reporting Items for Practice Guidelines in Healthcare (RIGHT) checklist. We assessed the compliance of the guidelines to each of the 35 items of RIGHT and summarized the reporting proportions of the seven domains of RIGHT.

Results: Eighteen guidelines were included. The mean proportion of appropriately reported RIGHT items was 52.4%. Among the seven domains of the RIGHT checklist, Basic information had the highest reporting rate (78.7%), and Review and quality assurance domain the lowest rate (16.7%). The domains Evidence (40.0%), Funding and declaration and management of interests (43.1%), and Other information (31.5%) had also reporting rates below 50%. Two RIGHT items (17 and 19b) were not reported by any of the guidelines.

Conclusions: The reporting quality of gastric cancer guidelines published in the years 2018-2020 was suboptimal, especially regarding the reporting of review, quality assurance and evidence. Guideline developers should pay attention on rigorous reporting following international standard to improve the quality of guidelines.
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http://dx.doi.org/10.21037/atm-21-2491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267280PMC
June 2021

Aging-related upregulation of the homeobox gene caudal represses intestinal stem cell differentiation in Drosophila.

PLoS Genet 2021 Jul 6;17(7):e1009649. Epub 2021 Jul 6.

Laboratory for Aging and Stem Cell Research, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

The differentiation efficiency of adult stem cells undergoes a significant decline in aged animals, which is closely related to the decline in organ function and age-associated diseases. However, the underlying mechanisms that ultimately lead to this observed decline of the differentiation efficiency of stem cells remain largely unclear. This study investigated Drosophila midguts and identified an obvious upregulation of caudal (cad), which encodes a homeobox transcription factor. This factor is traditionally known as a central regulator of embryonic anterior-posterior body axis patterning. This study reports that depletion of cad in intestinal stem/progenitor cells promotes quiescent intestinal stem cells (ISCs) to become activate and produce enterocytes in the midgut under normal gut homeostasis conditions. However, overexpression of cad results in the failure of ISC differentiation and intestinal epithelial regeneration after injury. Moreover, this study suggests that cad prevents intestinal stem/progenitor cell differentiation by modulating the Janus kinase/signal transducers and activators of the transcription pathway and Sox21a-GATAe signaling cascade. Importantly, the reduction of cad expression in intestinal stem/progenitor cells restrained age-associated gut hyperplasia in Drosophila. This study identified a function of the homeobox gene cad in the modulation of adult stem cell differentiation and suggested a potential gene target for the treatment of age-related diseases induced by age-related stem cell dysfunction.
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http://dx.doi.org/10.1371/journal.pgen.1009649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284806PMC
July 2021

Developing an integrated framework for source apportionment and source-specific health risk assessment of PAHs in soils: Application to a typical cold region in China.

J Hazard Mater 2021 08 24;415:125730. Epub 2021 Mar 24.

College of Water Sciences, Beijing Normal University, Beijing 100875, China; Engineering Research Center of Groundwater Pollution Control and Remediation, Ministry of Education, Beijing 100875, China. Electronic address:

Here, a new integrated methodology framework has been proposed for source apportionment and source-oriented risk evaluation, and applied to identify the characteristics, sources and health risks of PAHs in the soils of a typical cold region in Northeastern China. To this end, a large-scale data set containing 1780 soil samples and 16 priority PAHs has been collected from the study area. Two advanced receptor models, positive matrix factorization (PMF) and multivariate curve resolution-weighted alternating least-squares (MCR-WALS), have been comparatively employed to apportion the pollution sources of soil PAHs, with the help of a set of modified literature PAH source fingerprints. Further, the apportionment results have been incorporated into a probabilistic incremental lifetime cancer risk model for assessing the source-specific health risk of soil PAHs. Notably, the PMF and MCR-WALS models have apportioned essentially same results. The coal combustion and gasoline engine are identified as the main contributors of soil PAHs, with contributions of 57.9-58.1% and 25.2-22.2%, respectively. The health risks posed by PAHs in the soils are negligible for both adult and children; relatively, source-oriented risk assessment shows coal combustions make the largest contribution to the total risk of PAHs (56.1%), followed by gasoline engine (22.5%) and coke oven (21.4%).
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http://dx.doi.org/10.1016/j.jhazmat.2021.125730DOI Listing
August 2021

Rice SPL10 positively regulates trichome development through expression of HL6 and auxin-related genes.

J Integr Plant Biol 2021 Aug 4;63(8):1521-1537. Epub 2021 Aug 4.

State Key Laboratory of Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.

Trichomes function in plant defenses against biotic and abiotic stresses; examination of glabrous lines, which lack trichomes, has revealed key aspects of trichome development and function. Tests of allelism in 51 glabrous rice (Oryza sativa) accessions collected worldwide identified OsSPL10 and OsWOX3B as regulators of trichome development in rice. Here, we report that OsSPL10 acts as a transcriptional regulator controlling trichome development. Haplotype and transient expression analyses revealed that variation in the approximately 700-bp OsSPL10 promoter region is the primary cause of the glabrous phenotype in the indica cultivar WD-17993. Disruption of OsSPL10 by genome editing decreased leaf trichome density and length in the NIL-HL6 background. Plants with genotype OsSPL10 /HL6 generated by crossing WD-17993 with NIL-HL6 also had fewer trichomes in the glumes. HAIRY LEAF6 (HL6) encodes another transcription factor that regulates trichome initiation and elongation, and OsSPL10 directly binds to the HL6 promoter to regulate its expression. Moreover, the transcript levels of auxin-related genes, such as OsYUCCA5 and OsPIN-FORMED1b, were altered in OsSPL10 overexpression and RNAi transgenic lines. Feeding tests using locusts (Locusta migratoria) demonstrated that non-glandular trichomes affect feeding by this herbivore. Our findings provide a molecular framework for trichome development and an ecological perspective on trichome functions.
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http://dx.doi.org/10.1111/jipb.13140DOI Listing
August 2021

Integrating Metagenomic and Bayesian Analyses to Evaluate the Performance and Confidence of CrAssphage as an Indicator for Tracking Human Sewage Contamination in China.

Environ Sci Technol 2021 04 14;55(8):4992-5000. Epub 2021 Mar 14.

Engineering Research Center of Groundwater Pollution Control and Remediation, Ministry of Education; College of Water Sciences, Beijing Normal University, No. 19, Xinjiekouwai Street, Beijing 100875, P. R. China.

Recently, has been proposed as a human-specific marker for tracking fecal contamination. However, its performance has always been validated in a limited number of host samples, which may obscure our understanding of its utility. Furthermore, few studies have quantified confidence of fecal contamination when using . Here, we evaluate the performance and confidence of by analyzing a large panel of metagenomic data sets combined with Bayesian analyses. Results demonstrate that exhibits superior performance with high host sensitivity and specificity, indicating its suitability for tracking human fecal sources. With the marker, a high confidence (>90%) can be obtained and particularly, multiple samples with positive results provide a near certainty of confidence. The application of in the sediments of three Chinese urban rivers shows a high confidence of >97% of human fecal contamination, suggesting the serious challenge of sewage pollution in these environments. Additionally, significant correlation is observed between and antibiotic resistance genes (ARGs), expanding the utilization of for pollution management of ARGs. This study highlights the benefit of using metagenomic-based analysis for evaluating the performance and confidence of microbial source tracking markers in the coming era of big data with increasing resources in available metagenomic data.
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http://dx.doi.org/10.1021/acs.est.1c00071DOI Listing
April 2021

Epidermal growth factor upregulates the expression of A20 in hepatic cells via the MEK1/MSK1/p-p65 (Ser276) signaling pathway.

Am J Transl Res 2021 15;13(2):708-718. Epub 2021 Feb 15.

Department of Internal Medicine, Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University Zhengzhou 450008, Henan, P. R. China.

Tumor necrosis factor α-induced protein 3 (A20) suppresses inflammation by inhibiting the activation of nuclear factor kappa B (NF-κB). The aberrant expression of A20 is reportedly correlated with tumor development in human malignancies, including hepatocellular carcinoma (HCC). Proinflammatory mediators, including tumor necrosis factor α (TNF-α), interleukin-1, and lipopolysaccharide, may induce A20 expression. The present study revealed that epidermal growth factor (EGF) significantly increased A20 mRNA and protein levels in normal hepatic and hepatoma cells via the mitogen-activated protein kinase kinase-1 (MEK1)/mitogen- and stress-activated protein kinase-1 (MSK1)/phosphorylated (p)-p65 (Ser276) signaling pathway. A significant positive correlation was observed between the expression of EGF receptor and A20 in HCC and normal healthy liver tissues. The EGF-induced A20 upregulation was NF-κB-dependent and abolished by either the overexpression of the nuclear factor of a κ light polypeptide gene enhancer in a B-cell inhibitor α or treatment with the NF-κB inhibitor BAY11-7082. However, unlike TNF-α, EGF expression did not result in the upregulation of inflammatory molecules, including intercellular adhesion molecule 1, vascular cell adhesion molecule 1, and monocyte chemoattractant protein-1. These results indicate that EGF preferentially upregulated the protective mediator A20 over proinflammatory factors. To our knowledge, the present study is the first to demonstrate that EGF induced A20 expression by activating the MEK1/MSK1/p-p65 (Ser276) signaling pathway without causing an apparent inflammatory response. These results may further extend our understanding of liver inflammation and tumor development.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868826PMC
February 2021

Taurine represses age-associated gut hyperplasia in Drosophila via counteracting endoplasmic reticulum stress.

Aging Cell 2021 03 9;20(3):e13319. Epub 2021 Feb 9.

Laboratory for Stem Cell and anti-Aging Research, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, China.

As they age, adult stem cells become more prone to functional decline, which is responsible for aging-associated tissue degeneration and diseases. One goal of aging research is to identify drugs that can repair age-associated tissue degeneration. Multiple organ development-related signaling pathways have recently been demonstrated to have functions in tissue homeostasis and aging process. Therefore, in this study, we tested several chemicals that are essential for organ development to assess their ability to delay intestinal stem cell (ISC) aging and promote gut function in adult Drosophila. We found that taurine, a free amino acid that supports neurological development and tissue metabolism in humans, represses ISC hyperproliferation and restrains the intestinal functional decline seen in aged animals. We found that taurine represses age-associated ISC hyperproliferation through a mechanism that eliminated endoplasmic reticulum (ER) stress by upregulation of the target genes of unfolded protein response in the ER (UPR ) and inhibiting the c-Jun N-terminal kinase (JNK) signaling. Our findings show that taurine plays a critical role in delaying the aging process in stem cells and suggest that it may be used as a natural compound for the treatment of age-associated, or damage-induced intestinal dysfunction in humans.
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http://dx.doi.org/10.1111/acel.13319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7963329PMC
March 2021

Coexistence of a novel CCNY-ALK and ATIC-ALK double-fusion in one patient with ALK-positive NSCLC and response to crizotinib: a case report.

Transl Lung Cancer Res 2020 Dec;9(6):2494-2499

Department of Internal Medicine, Henan Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, China.

Anaplastic lymphoma kinase (ALK) rearrangement, one of the common oncogene rearrangements in the mutational history of lung adenocarcinoma, occurs in approximately 5% of non-small cell lung cancer (NSCLC) patients who could be effectively treated with ALK tyrosine kinase inhibitors (TKIs). The earlier phase III PROFILE 1014 study has shown that crizotinib, a first-generation ALK-TKI, significantly improved progression-free survival (PFS) compared with platinum-based chemotherapy in patients with previously untreated advanced ALK-positive NSCLC. Thus, clinicians must screen potential candidates for this driver alteration to guide ALK inhibitor therapy with a molecular testing platform capable of capturing all ALK fusions. Echinoderm microtubule-associated proteins, including the gene, are the most common ALK rearrangement partner. With the widespread use of the next-generation sequencing (NGS) techniques, which could approach enable the simultaneous screening of multiple genetic alterations, increasingly ALK rearrangement partners have been documented. However, the concurrent two ALK rearrangements within the same patient have rarely previously been reported. Here, we describe a novel CCNY-ALK (C1:A20) and ATIC-ALK (A7:A20), coexisting in the same case with poorly differentiated NSCLC and providing evidence of its sensitivity to ALK inhibitors. The newly identified rearrangement partners can be added to the list of ALK rearrangements that occurred in ALK-positive NSCLC, as it could lead to prolonged disease control. Also, while different ALK rearrangement variants might bring differing clinical outcomes, we discuss the impact of the co-mutations of these two ALK rearrangements on the sensitivity to ALK inhibitors. However, the impact of co-mutations on the pathogenesis of NSCLC should be further studied to supply more theoretical insight that co-mutations present for personalized anti-cancer therapy.
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http://dx.doi.org/10.21037/tlcr-20-1049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815378PMC
December 2020

Source apportionment of heavy metals in sediments and soils in an interconnected river-soil system based on a composite fingerprint screening approach.

J Hazard Mater 2021 06 14;411:125125. Epub 2021 Jan 14.

College of Water Sciences, Beijing Normal University, Beijing 100875, China. Electronic address:

Heavy metal pollution has been a global concern and key points of environmental pollution prevention and control due to the growing problems of urbanization and industrialization. Rapidly and correctly apportioning sources of heavy metal is still a great challenge because of the stability of source fingerprint and complex interaction of multiple contaminants and sources. In this study, we perform a combination of optimization of pollution source fingerprint and source apportionment through jointly utilizing two machine classification and screening methods for characterizing the pollution sources of heavy metal in the sediments of an urban river and its surrounding soils. Dominance-based rough set model (DRS), content optimization tools, and multivariate curve resolution-alternating least squares model (MCR-WALS) were employed to screen representative pollution source samples, optimize pollution source fingerprint, and apportion the potential sources of heavy metals, respectively. Further, Support vector machine (SVM) was adopted to correspondence analysis results and pollution fingerprint based on the factor characteristics for achieving source apportionment accurately. Results showed that the pollution source pollution source fingerprints optimized by DRS and optimization tools are more representative and stable, and the results obtained by SVM and MCR-WALS are more accurate comparing with traditional methods. As whole, source apportionment suggested that printing and dyeing, chemical, electroplating, metal processing were the main origins of heavy metals in this area and the proportions of them in sediment and soil pollution sources were 67.05% and 28.43%, respectively. Besides, coal combustion was also the main sources of heavy metal pollution in soils, accounting about 34.16%. Results of the study can advance our knowledge to better understand the characterization of heavy metal pollution in the peri-urban ecosystem and to design effective targeted strategies for reducing heavy metal pollution diffusion.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125125DOI Listing
June 2021

Environmental risk characterization and ecological process determination of bacterial antibiotic resistome in lake sediments.

Environ Int 2021 02 29;147:106345. Epub 2020 Dec 29.

Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China. Electronic address:

The increasing prevalence of antibiotic resistance genes (ARGs) in aquatic environments has attracted considerable concerns due to their potential threat to public health. For reducing environmental risk of ARGs, it is crucial to identify the pathogenic resistant bacteria, determine the driving forces governing the ARG community and apportion their sources, which is yet remained to explore. In this study, we developed a framework integrating high-throughput sequencing (HTS) analyses, null-model-based methods and machine-learning classification tool for understanding the environmental resistome risk and the ecological processes that control the ARG profile in aquatic sediments, and applied to two urban lakes (Lake Tai and Lake Baiyang) in China. The HTS-based metagenomic analyses revealed abundant and diverse resistome, mobilome and virulome in the two lakes, including some emerging ARGs such as mcr and carbapenemases types. Relatively, the diversities for ARGs, mobile genetic elements (MGEs) and virulence factor genes in Lake Baiyang were significantly higher than those in Lake Tai (p < 0.05). The metagenomic assembly and binning approaches tracked a number of potential pathogenic antibiotic resistant bacteria and found the co-occurrence of ARGs, MGEs and human bacterial pathogens in ~50% of the sediment samples, indicating a substantial resistome risk in the lakes. Comparison of multiple-site beta-diversity dissimilarity indexes suggested the ARG diversity was mainly explained by the spatial turnover rather than nestedness and exhibited significant distance-decay pattern. The results of using a novel null-model-based stochasticity ratio showed the stochastic processes made a higher contribution than the deterministic processes on the ARG profile in the environment, especially for Lake Baiyang (>65%). This was confirmed by the determination analyses of various ecological processes on ARG community by utilizing the null-model-based statistical framework for quantifying community assembly. That is, homogenizing dispersal (40%) dominated in Lake Baiyang, followed by homogeneous selection (32%) and ecological drift (15%), while ecological drift (33%) and homogenizing dispersal (31%) were the dominators in Lake Baiyang. SourceTracker analysis showed human sewage-associated sources were the largest contributor (~62%) of ARGs in the environment. The findings shed light on the dissemination risk and driver dynamics of antimicrobial resistance in the aquatic environment, which may help to make effective management strategies for controlling pollution of ARGs.
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http://dx.doi.org/10.1016/j.envint.2020.106345DOI Listing
February 2021

A coupled optimization of groundwater remediation alternatives screening under health risk assessment: An application to a petroleum-contaminated site in a typical cold industrial region in Northeastern China.

J Hazard Mater 2021 04 13;407:124796. Epub 2020 Dec 13.

Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081 China. Electronic address:

Contaminated sites have been recognized as posing serious comprehensive social and environmental issues and have earned worldwide attention. China is becoming one of the largest contaminated sites remediation markets in the world and the contaminated sites in northeastern China need to rehabilitate urgently. However, remediation planning is often hindered by high financial costs resulting from incomplete assessments of pollution and inappropriate remediation plans. In-depth contaminated site assessments can provide the necessary baseline data for remediation alternatives screening. Therefore, risk assessments and remediation decisions will play crucial roles in the rehabilitation and reconstruction of contaminated sites in China. The main objectives of this study were to present a novel method for health risk assessment (HRA) and to demonstrate a multicriteria decision analysis (MCDA) based on this method to select the most suitable remediation alternatives of groundwater and to prioritize management of contaminated site. To demonstrate the HRA and MCDA processes, a typical contaminated site in Longtan, Jilin province, China, was used. The results of this research indicated that Benzene (PhH) and 1,2-Dichloroethylene (1,2-DCE) were the main organic pollutants and the vanillin plant in the north of the site was main pollution source. Pollution migrated from the north to the south and the health risk range in winter was significantly greater than in summer. Four remediation alternatives were proposed on the basis of the HRA results. The MCDA results showed that PRB was the most suitable technology for integrating the relevant environmental, social, economic, and technical aspects required for remediation. This study may help responsible agencies to strengthen local risk-based program screening frameworks for contaminated sites, to promote reconstruction projects, and to increase local public confidence of contaminated sites remediation.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124796DOI Listing
April 2021

The Inhibition of H1N1 Influenza Virus-Induced Apoptosis by Surface Decoration of Selenium Nanoparticles with β-Thujaplicin through Reactive Oxygen Species-Mediated AKT and p53 Signaling Pathways.

ACS Omega 2020 Dec 16;5(47):30633-30642. Epub 2020 Nov 16.

Center Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 318 Renminzhong Road, Yuexiu District, Guangzhou 510120, Guangdong, People's Republic of China.

β-Thujaplicin possess a variety of biological activities. The use of modified biological nanoparticles (NPs) to develop novel anti-influenza drugs has increased in recent years. Selenium nanoparticles (SeNPs) with antiviral activity have attracted increasing attention for biomedical intervention. Functionalized SeNPs by β-thujaplicin ([email protected]) surface modified with superior antiviral activity were synthesized in this study. Compared to a virus group (43%), when treated with [email protected] (88%), the cell survival rate of MDCK cells was 45% higher. [email protected] could inhibit H1N1 from infecting Madin-Darby canine kidney (MDCK) cells and block chromatin condensation and DNA fragmentation. [email protected] obviously prevented MDCK cells from generating reactive oxygen species. Furthermore, [email protected] prevents lung injury in H1N1-infected mice through eosin staining and hematoxylin . Mechanistic investigation revealed that [email protected] inhibited H1N1 influenza virus from infecting MDCK cells through induction of apoptosis via suppressing AKT and p53 signaling pathways through immunohistochemical assay. Our results suggest that β-thujaplicin-modified SeNPs as carriers are an efficient way to achieve an antiviral pharmaceutical candidate for H1N1 influenza.
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http://dx.doi.org/10.1021/acsomega.0c04624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711941PMC
December 2020

The pathogenesis of renal injury in obstructive jaundice: A review of underlying mechanisms, inducible agents and therapeutic strategies.

Pharmacol Res 2021 01 24;163:105311. Epub 2020 Nov 24.

Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian 116011, China. Electronic address:

Kidney injury is one of the main complications of obstructive jaundice (OJ) and its pathogenesis has not been clarified. As an independent risk factor for OJ associated with significant morbidity and mortality, it can be mainly divided into two types of morphological injury and functional injury. We called these dysfunctions caused by OJ-induced kidney injury as OJKI. However, the etiology of OJKI is still not fully clear, and research studies on how OJKI becomes a facilitated factor of OJ are limited. This article reviews the underlying pathological mechanism from five aspects, including metabolisms of bile acids, hemodynamic disturbances, oxidative stress, inflammation and the organic transporter system. Some nephrotoxic drugs and measures that can enhance or reduce the renal function with potential intervention in perioperative periods to alleviate the incidence of OJKI were also described. Furthermore, a more in-depth study on the pathogenesis of OJKI from multiple aspects for exploring more targeted treatment measures were further put forward, which may provide new methods for the prevention and treatment of clinical OJKI and improve the prognosis.
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http://dx.doi.org/10.1016/j.phrs.2020.105311DOI Listing
January 2021

The inhibition of enterovirus 71 induced apoptosis by Durvillaea antarctica through P53 and STAT1 signaling pathway.

J Med Virol 2021 06 17;93(6):3532-3538. Epub 2020 Dec 17.

Center Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

The infection of enterovirus 71 (EV71) resulted in hand, foot, and mouth disease and may lead to severe nervous system damage and even fatalities. There are no effective drugs to treat the EV71 virus and it is crucial to find novel drugs against it. Polysaccharide isolated from Durvillaea antarctica green algae has an antiviral effect. In this study, D. antarctica polysaccharide (DAPP) inhibited the infection of EV71 was demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), reverse transcription polymerase chain reaction, flow cytometry, and western blot. MTT assay showed that DAPP had no toxicity on Vero cells at the concentration 250 μg/ml. Furthermore, DAPP significantly reduced the RNA level of EV71 in a dose-dependent manner. Moreover, DAPP inhibited the Vero cells apoptosis induced by EV71 via the P53 signaling pathway. Meanwhile, the expression of signal transducer and activator of transcription 1 and mammalian target of rapamycin were increased and the proinflammatory cytokines were significantly inhibited by DAPP. Taken together, these results suggested that DAPP could be a potential pharmaceutical against the infection of EV71 virus.
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http://dx.doi.org/10.1002/jmv.26693DOI Listing
June 2021

Effects on microbiomes and resistomes and the source-specific ecological risks of heavy metals in the sediments of an urban river.

J Hazard Mater 2021 05 4;409:124472. Epub 2020 Nov 4.

College of Water Sciences, Beijing Normal University, Beijing 100875, China; Engineering Research Center of Groundwater Pollution Control and Remediation, Ministry of Education, Beijing 100875, China. Electronic address:

This study aims to better understand the effects of heavy metal enrichment on microbiomes and resistomes and the source-specific ecological risks of metals in the sediments of an urban river. Geo-accumulation index and enrichment factor suggested the river sediments were contaminated by Cd, Cu, Pb, and Zn in varying degrees. High-throughput sequencing-based metagenomics analysis identified 430 types of antibiotic resistance genes (ARGs), dominated by the multidrug, MLS, bacitracin, quinolone, and aminoglycoside ARGs, and 52 metal resistance genes (MRGs) mainly conferring resistance to zinc, copper, cadmium, lead, mercury and multiple metals. Spearman correlation analysis and Mantel test showed the heavy metal enrichment exerted significant effects on the microbial community, ARGs and MRGs. Source apportionment using positive matrix factorization revealed that natural source (42.8%) was the largest contributor of metals in the river sediments, followed by urban activities (35.4%) and a mixed source (21.7%). However, when incorporating the apportionment results into a modified risk model to evaluate the source-specific ecological risks, results showed human activities dominated the risks of metals. Comparatively, the urban activities majorly caused moderate- and considerable- ecological risks, while the mixed source with respect to agricultural and industrial activities contributed higher percentages on high- and extremely high- ecological risks.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124472DOI Listing
May 2021

Intestinal barrier damage, systemic inflammatory response syndrome, and acute lung injury: A troublesome trio for acute pancreatitis.

Biomed Pharmacother 2020 Dec 2;132:110770. Epub 2020 Oct 2.

Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, PR China; Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, 116044, PR China. Electronic address:

Severe acute pancreatitis (SAP), a serious inflammatory disease of the pancreas, can easily lead to systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndromes (MODS). Acute lung injury (ALI) is one of the most serious complications of SAP. However, the specific pathogenesis of SAP-associated ALI is not fully understood. Crosstalk and multi-mechanisms involving pancreatic necrosis, bacteremia, intestinal barrier failure, activation of inflammatory cascades and diffuse alveolar damage is the main reason for the unclear pathological mechanism of SAP-associated ALI. According to previous research on SAP-associated ALI in our laboratory and theories put forward by other scholars, we propose that the complex pattern of SAP-associated ALI is based on the "pancreas-intestine-inflammation/endotoxin-lung (P-I-I/E-L) pathway". In this review, we mainly concentrated on the specific details of the "P-I-I/E-L pathway" and the potential treatments or preventive measures for SAP-associated ALI.
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http://dx.doi.org/10.1016/j.biopha.2020.110770DOI Listing
December 2020

Colorectal cancer under 20 years old: a retrospective analysis from three tertiary hospitals.

J Cancer Res Clin Oncol 2021 Apr 23;147(4):1145-1155. Epub 2020 Sep 23.

Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, 510060, China.

Purpose: Colorectal cancer (CRC) rarely occurs in children and adolescents. This study aimed to perform a retrospective analysis and disclose more detailed information about CRC in patients under 20 years old.

Methods: Medical records of CRCs in patients under 20 years old referred to three tertiary hospitals in China from September 2000 to July 2019 were retrospectively reviewed. Clinicopathological characteristics, treatment processes and laboratory findings were summarized and treatment outcomes and prognostic factors were analyzed.

Results: A total of 33,394 CRC medical records were analyzed, and we identified seventy (0.21%) CRCs in patients under 20. The most common primary tumor location was the left hemicolon (35.7%). The prominent pathological types were mucinous adenocarcinoma (22.9%) and signet ring cell carcinoma (22.9%). Nearly half (47.1%) of the patients presented with distant metastasis at diagnosis. The fractions of patients with deficient mismatch repair (dMMR) protein expression and microsatellite instability-high (MSI-H) were 23.8% (5/21) and 71.4% (5/7), respectively. Forty-four patients underwent radical surgery. Fifty-five patients received chemotherapy and six patients received radiotherapy. One dMMR/MSI-H rectal cancer patient received immunotherapy and achieved a clinically complete response. The median overall survival (OS) time was 80 months. The 3-year and 5-year OS rates were 61.8% and 57.2%, respectively. An absence of distant metastasis was a favorable factor for OS. For stage II/III CRCs, classic adenocarcinoma and radical surgery were favorable factors for OS. For stage IV CRCs, primary location at the colon was a favorable factor for OS.

Conclusion: Child and adolescent CRC patients are likely to have distant metastasis, undifferentiated, left hemicolon location, and a dMMR/MSI-H phenotype at diagnosis. Additional efforts are needed to improve their survival outcomes.
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http://dx.doi.org/10.1007/s00432-020-03397-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954766PMC
April 2021

Epstein-Barr virus infection in ulcerative colitis: a clinicopathologic study from a Chinese area.

Therap Adv Gastroenterol 2020 18;13:1756284820930124. Epub 2020 Aug 18.

Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, NO.58 Zhongshan Road II, Guangzhou 510080, Guangdong Province, P.R. China.

Background: Opportunistic Epstein-Barr virus (EBV) infection in patients with ulcerative colitis (UC) has attracted increasing attention. This study aimed to evaluate the clinicopathological characteristics and clinical outcomes of UC with intestinal EBV infection and to explore the predictive value of blood EBV DNA for the presence of EBV in the intestine.

Methods: Both peripheral blood and intestinal biopsies from 92 consecutive UC inpatients were included in this study. Normal colonic mucosal tissues from 20 colon cancer patients were used as controls. EBV testing and assessment were performed by EBV-DNA polymerase chain reaction (PCR), EBV-encoded small RNA hybridization (EBER-ISH) and immunohistochemistry.

Results: A total of 36 patients (39.1%) had UC with superimposed EBV colitis [EBER greater than 2/high-power field (HPF)]. EBER counts and disease activity were significantly correlated ( < 0.05). The major endoscopic findings revealed more irregular and longitudinal ulcers in patients with superimposed EBV colitis ( = 0.016,  = 0.021, respectively). Age, steroid dependence, and irregular ulcerations were identified as possible risk factors. The best EBER cut-off point for outcome prediction was 2.5/HPF. At a cut-off value of 2035 copies/ml, the sensitivity and specificity of the blood EBV-DNA PCR analysis for predicting EBV presence in the intestine were 76.5% and 68.5%, respectively. EBV-infected cells in UC with high EBV concentrations mainly included B lymphocytes by clinicopathology, and the infection might have progressed from the latent to the lytic phase of the EBV life cycle.

Conclusion: The EBER count is positively correlated with disease activity. The best cut-off point for outcome prediction is 2.5/HPF. A high EBV viremia load may effectively predict EBV presence in the colonic mucosa.
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http://dx.doi.org/10.1177/1756284820930124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444145PMC
August 2020

Source apportionment and source-oriented risk assessment of heavy metals in the sediments of an urban river-lake system.

Sci Total Environ 2020 Oct 17;737:140310. Epub 2020 Jun 17.

Engineering Research Center of Ministry of Education on Groundwater Pollution Control and Remediation, College of Water Sciences, Beijing Normal University, Beijing, 100875, China.

Heavy metal pollution in lakes has attracted concerns worldwide since long retention times in lakes allow metals to accumulate and may pose significant threat to ecosystem health. For designing targeted risk mitigation strategies, it is necessary to identify the source-specific risks of heavy metals in the environment. Although previous studies have addressed either risk assessment or source identification of heavy metals in the environment, few have attempted to establish a link between them. In the study, we perform a combination of source apportionment and risk assessment for characterizing the pollution sources and source-specific risks of heavy metals in the sediments of an urban river-lake system. To this end, positive matrix factorization (PMF) was employed to apportion the potential sources of heavy metals, combined with a support vector machine classifier and the referential source fingerprints of metals in the study area. Then, the apportionment results were incorporated into the environmental risk models to evaluate the ecological and human health risks posed by heavy metals from the identified pollution sources. Results showed the river-lake system was contaminated by Cd, Cu, Zn and other metals in varying degree. Particularly, the element of Cd presented moderate to heavy pollution level. In relative, the industrial activities were identified as the largest contributor (48.0%) of heavy metals in the river-lake sediments, mainly associating with electroplating and paper making, followed by the agricultural activities (27.3%) and mix source (24.7%). Overall, the non-carcinogenic and carcinogenic risks posed by the heavy metals were acceptable, however, the element of Cd showed moderate ecological effect. Further, source-oriented risk evaluation suggested industrial processes made higher contributions to the ecological risk of heavy metals in the river-lake system. The study will provide regulators help to update the information by adding apportionment analysis in the context of risk assessment to facilitate subsequent mitigation strategies.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140310DOI Listing
October 2020

Association between adjuvant chemotherapy and survival in patients with rectal cancer and pathological complete response after neoadjuvant chemoradiotherapy and resection.

Br J Cancer 2020 10 29;123(8):1244-1252. Epub 2020 Jul 29.

Department of Radiation Oncology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: For patients with locally advanced rectal cancer (LARC), it is unclear whether neoadjuvant chemoradiotherapy-induced pathologic complete response (pCR) individuals would further benefit from adjuvant chemotherapy (ACT).

Methods: The pCR individuals who received different ACT cycles were paired by propensity score matching. Overall survival (OS), disease-free survival (DFS), local recurrence-free survival (LRFS), and distant metastasis-free survival (DMFS) were calculated by Kaplan-Meier and log-rank test.

Results: In total, 1041 pCR individuals were identified from 5567 LARC cases. Specifically, 303 pCR cases had no ACT treatment, and 738 pCR patients received fluoropyrimidine-based ACT (median, 4 cycles) treatment. After 1:3 propensity score matching, 297 cases without ACT treatment were matched to 712 cases who received ACT treatment. Kaplan-Meier analysis showed that pCR individuals treated with or without ACT had the similar 3-year outcome (OS, DFS, LRFS and DMFS) (all P > 0.05). Moreover, the pCR patients received different ACT cycle(s) (0 vs. 1-4 cycles, 0 vs. ≥5 cycles) had comparable 3-year OS, DFS, LRFS and DMFS (all P > 0.05). In stratified analysis, ACT treatment did not improve 3-year survival (OS, DFS, LRFS and DMFS) for the baseline high-risk (cT3-4/cN1-2) subgroup patients (all P > 0.05).

Conclusion: ACT, which did not improve survival, is unnecessary to neoadjuvant treatment-induced pCR LARC patients.

Trial Registration: 2019ZSLYEC-136 (24-6-2019).
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http://dx.doi.org/10.1038/s41416-020-0989-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553967PMC
October 2020

Next-Generation Sequencing Identified a Novel Crizotinib-Sensitive PLB1-ALK Rearrangement in Lung Large-Cell Neuroendocrine Carcinoma.

Clin Lung Cancer 2021 05 2;22(3):e366-e370. Epub 2020 Jun 2.

Department of Internal Medicine, Henan Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.cllc.2020.05.026DOI Listing
May 2021

Lipoic acid rejuvenates aged intestinal stem cells by preventing age-associated endosome reduction.

EMBO Rep 2020 08 9;21(8):e49583. Epub 2020 Jul 9.

Laboratory for Stem Cell and Anti-Aging Research, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, China.

The age-associated decline of adult stem cell function is closely related to the decline in tissue function and age-related diseases. However, the underlying mechanisms that ultimately lead to the observed functional decline of stem cells still remain largely unexplored. This study investigated Drosophila midguts and found a continuous downregulation of lipoic acid synthase, which encodes the key enzyme for the endogenous synthesis of alpha-lipoic acid (ALA), upon aging. Importantly, orally administration of ALA significantly reversed the age-associated hyperproliferation of intestinal stem cells (ISCs) and the observed decline of intestinal function, thus extending the lifespan of Drosophila. This study reports that ALA reverses age-associated ISC dysfunction by promoting the activation of the endocytosis-autophagy network, which decreases in aged ISCs. Moreover, this study suggests that ALA may be used as a safe and effective anti-aging compound for the treatment of ISC-dysfunction-related diseases and for the promotion of healthy aging in humans.
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http://dx.doi.org/10.15252/embr.201949583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403706PMC
August 2020

Inhibition of Enterovirus 71 by Selenium Nanoparticles Loaded with siRNA through Bax Signaling Pathways.

ACS Omega 2020 Jun 13;5(21):12495-12500. Epub 2020 May 13.

Center Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 318 Renminzhong Road, Yuexiu District, Guangzhou 510120, Guangdong, People's Republic of China.

Enterovirus 71 (EV71) is the principal pathogen leading to severe cases of hand, foot, and mouth disease (HFMD). Specific drugs for EV71 are not discovered currently. Small interfering RNA (siRNA) provides a promising antiviral treatment pathway, but it is difficult to cross cell membranes and is easy to degrade. Nanoparticles are promising for their carrying capacity currently. In this study, the siRNA targeting EV71 VP1 gene was loaded with selenium nanoparticles (SeNPs) and surface decorated with polyethylenimine (PEI) ([email protected]@siRNA). [email protected]@siRNA showed a remarkable interference efficiency in the nerve cell line SK-N-SH and prevented the cells to be infected. The mechanism study revealed that [email protected]@siRNA could lighten the extent of SK-N-SH cells for staying in the sub-G1 phase. Activation of Bax apoptosis signaling was restrained either. Taken together, this study demonstrated that [email protected]@siRNA is a promising drug against EV71 virus.
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http://dx.doi.org/10.1021/acsomega.0c01382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271353PMC
June 2020

When cancer encounters COVID-19 in China: what have we suffered, experienced and learned.

Jpn J Clin Oncol 2020 Jun;50(6):712-717

Department of Internal Medicine, Henan Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, China.

The outbreak of the coronavirus disease (COVID-19) occurred in Wuhan, China, in December 2019. As of 21 March 2020, this epidemic has spread to 179 countries with more than 200 000 confirmed cases and 8578 deaths. The outbreak has put enormous pressure on the medical establishment and even led to exhaustion of medical resources in the most affected areas. Other medical work has been significantly affected in the context of COVID-19 epidemic. In order to reduce or avoid cross-infection with COVID-19, many hospitals have taken measures to limit the number of outpatient visits and inpatients. For example, emergency surgery can only be guaranteed, and most other surgeries can be postponed. Patients with cancer are one of the groups most affected by the epidemic because of their systematic immunosuppressive state and requirement of frequent admission to hospital. Consequently, specific adjustments for their treatment need to be made to cope with this situation. Therefore, it is of significance to summarize the relevant experience of China in the prevention and control of COVID-19 infection and treatment of patients with cancer during the epidemic.
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http://dx.doi.org/10.1093/jjco/hyaa077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239118PMC
June 2020

Ecotoxicological risk assessment and source apportionment of antibiotics in the waters and sediments of a peri-urban river.

Sci Total Environ 2020 Aug 6;731:139128. Epub 2020 May 6.

Engineering Research Center of Groundwater Pollution Control and Remediation, Ministry of Education, College of Water Sciences, Beijing Normal University, No 19, Xinjiekouwai Street, Beijing 100875, China.

Antibiotics have been widely used in the past decades and caused global public health concerns due to the growing problems of antimicrobial resistance. The peri-urban rivers are always receiving massive wastes containing antibiotics and appear to be a reservoir of antibiotics and antibiotic resistance genes in the environment. To prevent and control the pollution of antibiotics, it is essential to correctly identify the potential sources of antibiotics in peri-urban rivers. Currently, systematic knowledge on risk characteristics and source apportionment of antibiotics in peri-urban rivers is still lacking. In the study, we addressed this problem and focused on exploring the ecotoxicological risk and potential sources of antibiotics in a peri-urban river in Beijing (Chaobai River). To this end, the waters and sediments were collected from the river, as well as the potential source types including domestic sewage, WWTP effluent, chicken manure, pig manure and cattle manure. The occurrence and concentration levels of 16 antibiotics in the waters and sediments of the river were comprehensively characterized, as well as the correlation of antibiotics with environmental factors. Then, risk quotients and mixture risk quotients were used to assess the ecotoxicological risk of single compound and the mixture toxicity of antibiotics, respectively. The synergistic effects of antibiotic mixtures were also analyzed. Further, positive matrix factorization was employed to apportion the potential sources of antibiotics based on the multilinear engine (ME-2) algorithm. The target antibiotics were widely detected in the peri-urban river and several antibiotics posed moderate ecotoxicological risks on aquatic organisms. Apportionment analysis identified four potential sources of antibiotics in the waters of Chaobai River, including domestic sewage (31.5%), chicken waste (26.4%), WWTP effluent (22.2%) and a mix source (20.0%). Additionally, WWTP effluent (~58%) and sewage effluent (41%) were apportioned as the main contributors of antibiotics in the sediments.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139128DOI Listing
August 2020

Peroxisome Elevation Induces Stem Cell Differentiation and Intestinal Epithelial Repair.

Dev Cell 2020 04 2;53(2):169-184.e11. Epub 2020 Apr 2.

Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong, China; Laboratory for Stem Cell and anti-Aging Research, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China. Electronic address:

Epithelial-repair-dependent mucosal healing (MH) is associated with a more favorable prognosis for patients with inflammatory bowel disease (IBD). MH is accomplished via repair and regeneration of the intestinal epithelium. However, the mechanism underlying MH is ill defined. We found a striking upregulation of peroxisomes in the injured crypts of IBD patients. By increasing peroxisome levels in Drosophila midguts, we found that peroxisome elevation enhanced RAB7-dependent late endosome maturation, which then promoted stem and/or progenitor-cell differentiation via modulation of Janus Kinase (JAK) and Signal Transducer and Activator of Transcription (STAT)-SOX21A signaling. This in turn enhanced ISC-mediated regeneration. Importantly, RAB7 and SOX21 were upregulated in the crypts of IBD patients. Moreover, administration of drugs that increased peroxisome levels reversed the symptoms of dextran sulfate sodium (DSS)-induced colitis in mice. This study demonstrates a peroxisome-mediated epithelial repair mechanism, which opens a therapeutic avenue for the enhancement of MH in IBD patients.
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http://dx.doi.org/10.1016/j.devcel.2020.03.002DOI Listing
April 2020
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