Publications by authors named "Haiyan Wu"

252 Publications

Epidemiological Analysis of Multidrug-Resistant Isolates in a Tertiary Hospital Over a 12-Year Period in China.

Front Public Health 2021 12;9:707435. Epub 2021 Aug 12.

Tai'an City Central Hospital, Taian, China.

is an important nosocomial pathogen, which is multidrug resistant (MDR). has become a major threat to public health worldwide due to its ability to easily acquire resistant genes. In order to analyze its epidemiology characteristics and the genetic evolution, isolates obtained from a Chinese tertiary hospital in the past 12 years (2008-2019), 295 isolates of non-repetitive , were recovered from patients and wards environments. The resistance genes were analyzed using antimicrobial susceptibility testing. The genetic relatedness of 295 isolates was identified by multilocus sequence typing (MLST) and eBURST analysis. It was found that the antibiotic-resistant and carbapenemase-resistant genes of all the 295 MDR in the hospital have not changed significantly over the past 12 years; all of them were resistant to multiple antibiotics except the polymyxin E and tigecycline. The results of drug-resistant genes showed that the detection rates of carbapenemase-resistant genes , , and were 97.6, 75.3, and 71.9%, respectively, which were detected almost every year from 2008 to 2019. Additionally, 16s rRNA methylation enzyme gene , aminoglycoside-resistant gene , and class I integrase gene could also have a high positive rate. By MLST, these isolates were assigned to 12 sequence types (STs), including ST369, ST208, ST195, ST191, ST368, ST530, ST469, ST451, ST229, ST381, ST543, and ST1176. eBURST analysis showed that 9 STs with ST208 as the founder genotype belonged to Group 1 except for ST229, ST530, and ST1176. Therefore, most MDR isolates had a relatively close genetic relationship. Notably, the predominant ST208 and ST369 at the early stage changed to ST451 in 2019, indicating that the complex and diverse genetic background of the prevalence of isolates in the hospital. Overall, further epidemiological surveillance and genetic evolution analysis of are required, which can provide new strategies for the prevention and control of infections.
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http://dx.doi.org/10.3389/fpubh.2021.707435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388840PMC
August 2021

Proteiniclasticum sediminis sp. nov., an obligate anaerobic bacterium isolated from anaerobic sludge.

Antonie Van Leeuwenhoek 2021 Oct 16;114(10):1541-1549. Epub 2021 Aug 16.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, People's Republic of China.

An obligate anaerobic bacterial BAD-10 was isolated from anaerobic acetochlor-degrading sludge. The strain was Gram-stain negative, curved rod-shaped, non-motile and non-spore-forming. Growth was observed in PYT medium at pH 6.0-9.0 (optimum, pH 7.5), at 25-47 °C (37 °C) and with 0-1.0% NaCl (w/v, 0%). Strain BAD-10 could degrade acetochlor. The major fermentation products from peptone-yeast (PY) medium were acetate and butyrate. The predominant cellular fatty acids were iso-C FAME, anteiso-C FAME and C FAME. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain BAD-10 showed closest affiliation to Proteiniclasticum ruminis D3RC-2, with a sequence similarity of 97.6%. Genome sequencing revealed a genome size of 2,983,986 bp, a G + C content of 51.4 mol% and protein-coding genes of 3,102. The average nucleotide identity and in silico DNA-DNA hybridization values between strain BAD-10 and Proteiniclasticum ruminis D3RC-2 were 71.0% and 20.4%, respectively, which were below the standard thresholds for species differentiation. On the basis of phenotypic, physiological and phylogenetic evidence, strain BAD-10 represents a novel species in the genus Proteiniclasticum, for which the name Proteiniclasticum sediminis sp. nov. is proposed. Strain BAD-10 (= CCTCC AB 2021091 = KCTC 25288) is the type strain of the proposed novel species.
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http://dx.doi.org/10.1007/s10482-021-01620-9DOI Listing
October 2021

Case Report/Case Series: Rare case of anti-LGI1 limbic encephalitis with rapidly progressive dementia, psychiatric symptoms, and frequently seizures: A case report.

Medicine (Baltimore) 2021 Jul;100(29):e26654

Psychiatry Department, Beijing Geriatric Hospital, Beijing, PR China.

Rationale: Anti leucine-rich glioma inactivated 1 (LGI1) limbic encephalitis (LE) is rare autoimmune encephalitis, characterized by acute or subacute cognitive impairment, faciobrachial dystonic seizures, mental disorders, and refractory hyponatremia. As a type of treatable rapidly progressive dementia with a good prognosis, early, and accurate diagnosis is essential. We present a case of anti-LGI1 LE who was initially misdiagnosed with Alzheimer disease because his clinical manifestations were similar to Alzheimer disease.

Patient Concerns: A male patient presenting with rapidly progressive dementia, faciobrachial dystonic seizures, psychiatric disturbance, and refractory hyponatremia was admitted. The scores of Mini-Mental State Examination, Montreal Cognitive Assessment, and Neuropsychiatric Inventory were 19/30, 16/30, and 91/144, respectively. Brain magnetic resonance images indicated moderate atrophy of the hippocampus and abnormally hyperintensities in the left medial temporal and hippocampus.

Diagnosis: The patient was diagnosed with anti-LGI1 LE based on the presence of LGI-1 antibodies in the cerebrospinal fluid and serum and clinical manifestations.

Interventions: Patient was treated with glucocorticoid against LGI1, antiepileptic drug, cholinesterase inhibitors, and other adjuvant therapy.

Outcomes: The patient showed marked improvement on immunotherapy. Clinical symptoms were disappeared and the LGI-1 antibodies in cerebrospinal fluid and serum were both negative at the time of discharge.

Conclusions: Recognition of the specific symptoms and LGI-1 antibody test will be helpful for the early diagnosis, prompt immunotherapy, and good prognosis. This case raises the awareness that rapidly progressive dementia with frequent seizures could be caused by immunoreactions.
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http://dx.doi.org/10.1097/MD.0000000000026654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294912PMC
July 2021

Tuning emission color and improving the warm-white persistent luminescence of phosphor BaLuAlGaSiO:Pr Zn co-doping.

Dalton Trans 2021 Sep 14;50(35):12137-12146. Epub 2021 Sep 14.

State key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

In this article, we synthesized a series of new warm-white emitting persistent luminescent phosphors by co-doping Zn into Pr activated BaLuAlGaSiO, and systematically investigated the effect of Zn co-doping on both their photoluminescence and persistent luminescence properties. Following the removal of UV excitation, the phosphor emits warm-white persistent luminescence consisting of greenish-blue and red emissions originating from P and D multiplet electron transitions at the 4f level of Pr. The luminescence properties of the BaZnLuAlGaSiO:Pr phosphors can be modified by changing the content of Ba/Zn in the host, which affects the non-radiative energy flow between 5d-P-D levels and resultantly enhances the intensity of the 4f → 4f transition. Compared with the undoped sample, Zn co-doping can significantly enhance the persistent luminescence intensity of the phosphors in the range of 400-800 nm and reduce the intensity in the UV region. Meanwhile, Zn co-doping can also change the intensity ratio between the greenish-blue and red emissions, and the persistent luminescence color can be tuned from red to warm-white with the increase of Zn concentration. Besides, the Zn ions entering the crystal lattice also enhance the persistent luminescence performance by modifying the defect levels in the phosphor. For the optimized phosphor, bright warm-white persistent luminescence can be observed by the naked eye in the dark after the removal of the excitation source for 4 h. Based on the experimental results, a feasible mechanism was also proposed to reveal the persistent luminescence generation process for the BaLuAlGaSiO:Pr,Zn phosphor.
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http://dx.doi.org/10.1039/d1dt01913aDOI Listing
September 2021

Variation p.R1045H in MYH7 correlated with hypertrophic cardiomyopathy in a Chinese pedigree.

BMC Med Genomics 2021 Jul 30;14(1):196. Epub 2021 Jul 30.

Department of Cardiology, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, No. 157 Jinbi Road, Kunming, 650032, Yunnan Province, China.

Background: Inherited hypertrophic cardiomyopathy (HCM) is a common heart muscle disease that damages heart function and may cause the heart to suddenly stop beating. Genetic factors play an important role in HCM. Pedigree analysis is a good way to identify the genetic defects that cause disease.

Methods: An HCM pedigree was determined in Yunnan, China. Whole-exome sequencing was performed to identify the genetic variants of HCM. Another 30 HCM patients and 200 healthy controls were also used to investigate the frequency of the variants by customized TaqMan genotyping assay.

Results: The variant NM_000257.4:c.3134G > A (NP_000248.2:p.Arg1045His, rs397516178, c.3134G > A in short) was found to cosegregate with the clinical phenotype of HCM. Moreover, the variant was not found in the 200 control subjects. After genotyping the variant in 30 HCM patients, there was one patient who carried the variant and had a family history.

Conclusions: Our findings suggest that this variant may be closely related to the occurrence of the disease. According the ACMG guidelines, the c.3134G > A variant should be classified as "Likely pathogenic".
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http://dx.doi.org/10.1186/s12920-021-01046-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325323PMC
July 2021

Inflammatory Myofibroblastic Tumors in Children: A Clinical Retrospective Study on 19 Cases.

Front Pediatr 2021 8;9:543078. Epub 2021 Jul 8.

Department of Cardiothoracic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.

Inflammatory myofibroblastic tumor (IMFT) is a rare neoplasm mainly affecting children and young adults. We conducted a retrospective study to evaluate the clinical features and treatment alternatives of childhood inflammatory myofibroblastic tumors. A total of 19 patients who were pathologically diagnosed with IMT between December 2008 and October 2018 were included. Collected data were demographic information, main complaints, tumor characteristics, treatment, pathological results, immunohistochemical analysis, and prognosis. The male/female ratio was 13:6. The mean age at disease onset was 44.9 ± 33.9 months (range 4 to 111 months). The mean tumor size was 6.5 ± 4.0 cm (range 1.2 to 17.0 cm). The most common site was the abdomen (13/19). The most commonly used detection tool was CT. Eleven patients (57.9%) had aggressive tumor growth, including eight receiving extensive resection and three receiving palliative resection due to high local invasiveness and postoperative chemotherapy. Eight cases whose tumors were completely enveloped received complete resection. Immunohistochemistry was performed for 17 patients and ALK positivity was found in 11 patients. Despite three children lost to follow-up, sixteen patients were followed up for 6 to 132 months (average 63.9 months, median 66 months). Of which, twelve children survived with no evidence of IMT, and four cases (21%) showed local recurrences (two of them died). No distant metastasis was detected. IMT is rare in children with various locations, mostly appearing in the abdomen. Whether the tumor could be completely removed, the location and the invasiveness of surrounding tissues might be highly prognosis-related.
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http://dx.doi.org/10.3389/fped.2021.543078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295553PMC
July 2021

[email protected] Nanoparticles for Photodynamic/Photothermal Combined Cancer Therapy with Single Near-Infrared Irradiation.

Int J Nanomedicine 2021 16;16:4863-4871. Epub 2021 Jul 16.

Laboratory of Veterinary Pathology and Nanopathology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.

Background: Phototherapy has significant potential as an effective treatment for cancer. However, the application of a multifunctional nanoplatform for photodynamic therapy (PDT) and photothermal therapy (PTT) at a single excitation wavelength remains a challenge.

Materials And Methods: The double emulsion solvent evaporation method was used to prepare toluidine [email protected] lactic-co-glycolic acid ([email protected]) nanoparticles (NPs). The biocompatibility of [email protected] NPs was evaluated, and a 660 nm luminescence was used as the light source. The photothermal effect, photothermal stability, and singlet oxygen yield of NPs in an aqueous solution verified the feasibility of NPs as a PTT/PDT synergistic therapy drug.

Results: [email protected] NPs were successfully prepared and characterized. In vitro experiments demonstrated that [email protected] NPs can cause massive necrosis of tumor cells and induce apoptosis through a photodynamic mechanism under 660 nm laser irradiation. The [email protected] NPs also achieved optimal tumor inhibition effect in vivo.

Conclusion: The [email protected] NPs prepared in this study were applied as a dual-mode phototherapeutic agent under single laser irradiation. Both in vitro and in vivo experiments demonstrated the good potential of PTT/PDT for tumor inhibitors.
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http://dx.doi.org/10.2147/IJN.S304713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291662PMC
July 2021

Cascade targeting tumor mitochondria with CuS nanoparticles for enhanced photothermal therapy in the second near-infrared window.

Biomater Sci 2021 Aug 23;9(15):5209-5217. Epub 2021 Jun 23.

i-Lab and Division of Nanobiomedicine, CAS Key Laboratory of Nano-Bio Interface, CAS Center for Excellence in Nanoscience, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.

Photothermal therapy, assisted by local heat generation using photothermal nanoparticles (NPs), is an emerging strategy to treat tumors noninvasively. To improve treatment outcomes and to alleviate potential side effects on normal tissue cells, utilizing the optically transparent second near-infrared (NIR-II) window and actively targeting tumors are critical. Considering that mitochondria are heat sensitive and play an important role in the up-regulation of metabolic activity in tumor cells, herein we report a cascade targeting scheme that enables active photothermal ablation of tumor mitochondria. First, NIR-II absorbing CuS NPs were surface modified with the mitochondria targeting moiety (3-carboxypropyl) triphenylphosphonium bromide (TPP) and then shielded with CD44 targeting hyaluronic acid, which will only expose TPP upon reaching the tumor sites. This allowed over 90% CuS NP enrichment at tumor mitochondria, and as a result, significantly improved tumor cell photothermal ablation was observed at the cellular level. An in vivo study demonstrated enhanced tumor uptake and improved tumor growth suppression by using these cascade targeting CuS NPs as NIR-II photothermal agents.
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http://dx.doi.org/10.1039/d1bm00589hDOI Listing
August 2021

Reward mechanism of depressive episodes in bipolar disorder: Enhanced theta power in feedback-related negativity.

J Affect Disord 2021 09 29;292:217-222. Epub 2021 May 29.

Division of Mood Disorder, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. Electronic address:

Introduction: This study aimed to explore the reward-related neural mechanism in patients with depressive mood in bipolar disorder (BD) using event-related potentials. It remains unknown whether or not different neurobiological markers underlying depression symptoms in BD depression and major depression disorder (MDD).

Methods: 24 patients with BD depression and 20 healthy controls were included. Participants underwent evaluation with the Temporal Experience of Pleasure Scale (TEPS), followed by the classical gambling paradigm, while undergoing 64-channel electroencephalography. The waveform of feedback-related negativity (FRN) was extracted from the 250-350 ms time-window after participants received feedback regarding loss or gain. Event-related potential datasets were obtained using time-frequency analysis.

Results: (1) The TEPS scores of the patients were significantly lower than those of the controls [t = 5.16, p < 0.01]. (2) The event of loss elicited a deeper FRN in patients than that in controls [t = 2.19, p < 0.05], while no difference was observed in the event of gains (t = 1.12, p > 0.05). (3) Theta power rooted in FRN in patients was significantly higher in loss than in gain [F = 30.32, p < 0.01]. (4) Analysis of Variance (ANOVA) illustrated the interaction effect of theta power in gain/loss between two groups [F = 3.59, p = 0.06].

Limitation: Our study did not analyze the effect of different drugs which might affect our results.

Conclusion: The enhanced reflection of negative feedback was consistent with the negative bias, impulse control impairment, and emotional dysregulation observed in the bipolar disorder spectrum. We suggested that the extreme theta power generated from the anterior cingulate gyrus (ACC) might be the main component of abnormal FRN.
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http://dx.doi.org/10.1016/j.jad.2021.05.057DOI Listing
September 2021

[Visual analysis of research on multidrug-resistant bacteria in intensive care unit in China].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 May;33(5):587-592

Department of Intensive Care Unit, Taian City Central Hospital, Taian 271000, Shandong, China.

Objective: To identify the distribution of research hotspots and frontiers of multidrug-resistant bacteria in intensive care units in China through the method of visualization, and to predict future research directions, analyze the research development process, so as to provide reference basis for further research in this field.

Methods: Studies related to multidrug-resistant bacteria in intensive care units published in China from 2000 to 2019 by CNKI were reviewed. According to the keywords by CiteSpace 5.6.R2, the co-occurring network was generated to analyze the distribution of research hotspots in this field. Meanwhile, the mutation map of keywords was used to forecast the future research directions to a certain extent.

Results: A total of 1 324 articles were finally included in the quantitative analysis. From 2000 to 2019, the number of publications in the field of multi-drug resistant bacteria in intensive care units showed a gradual increase, of which the number of publications increased rapidly from 2008 to 2014 (the number of publications increased from 34 to 124 articles). In the initial stage of research, conceptual keywords appeared, such as intensive care unit, infection, pathogenic bacteria and drug resistance among others. A dense keywords group appeared from 2008 to 2014, which covered several aspects, such as nosocomial infection, Acinetobacter baumannii, pathogenic bacteria, drug resistance, pulsed field gel electrophoresis and nursing etc., suggesting that research in this field has entered a period of vigorous development. High-frequency keywords, such as risk factors, pathogens, antibiotics, nosocomial infections, drug resistance genes and homology appeared from 2015 to 2019, thus representing the hotspots in recent years.

Conclusions: The overall research on multidrug-resistant bacteria in intensive care units in China has gradually improved. The current studies focus on homology analysis and multidrug-resistant bacteria infections, among other topics. Further explorations at the genetic level will be conducted to fill the research vacancy in this field and to provide molecular biological basis for reducing the occurrence of multidrug-resistant bacteria in the future.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200915-00626DOI Listing
May 2021

Soil bacterial community dynamics following bioaugmentation with Paenarthrobacter sp. W11 in atrazine-contaminated soil.

Chemosphere 2021 Nov 28;282:130976. Epub 2021 May 28.

Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, 1363 Shengtai Street, Changchun, 130033, China. Electronic address:

Atrazine is one of the most widely used herbicides, however it and its metabolites cause widespread contamination in soil and ground water. Bioaugmentation is an effective method for remediation of environmental organic pollutants. High-throughput sequencing provides an important tool for understanding the changes of microbial community and function in response to pollutants degradation based on bioaugmentation. In this study, the effect of biodegradation with Paenarthrobacter sp. W11 and the change of microbial community during atrazine degradation were investigated. The results showed that bioaugmentation significantly accelerated the degradation rate of atrazine in soil and reduced the toxic effect of atrazine residues on wheat growth. The extra available NH through atrazine mineralization could serve as a nitrogen source to increase microbial numbers. High-throughput sequencing further revealed that the microbial community restored a new balance. The function of microbial community predicted by PICRUSt2 suggested that the biodegradation process of atrazine affected not only the atrazine degradation pathway, but also the nitrogen metabolism pathway. Methylobacillus and Pseudomonas were considered as the most important indigenous atrazine-degrading microorganisms, because their relative abundances were positively correlated with the relative abundance of Paenarthrobacter and atrazine degradation pathway. This study provides insight into the cooperation between indigenous microorganisms and external inoculums on atrazine degradation process.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130976DOI Listing
November 2021

[Chronic venous disease increases placental villous angiogenesis and lymphangiogenesis and villous cell apoptosis].

Authors:
Haiyan Wu

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Jun;37(6):538-545

Department of Obstetrics, Northwest Women's and Children's Hospital, Xi'an 710003, China. *Corresponding author, E-mail:

Objective To investigate the changes of key factors such as placental villi and lymph vessels in chronic venous disease (CVD) during pregnancy. Methods According to the CEAP classification criteria, tissues of CVD patients were collected, and was divided into control group and CVD group. Real-time quantitative PCR was used to detect mRNA level of CD31, D2-4, fms-related tyrosine kinase 1 (FLT-1), placental growth factor (PlGF) and hypoxia-inducible factor-1 (HIF-1α) in placental tissues. Immohistochemical staining was performed to measure the expression level of CD31, D2-40, FLT-1, PlGF, HIF-1α, B-cell lymphoma 2 (Bcl2), Bcl2-related X protein (BAX), caspase 3 and caspase-9. The change in average number of syncytiotrophoblast cell nodes in placental villi was observed by optical microscope and transmission electron microscope. Periodic acid Schiff (PAS) staining was used to detect the PAS-positive substances in placental villi. Results Compared with the control group, the average numbers of placenta villi, syncytiotrophoblast cell nodes, syncytiotrophoblast cell nodes/villi and the connection bridges between villi of the average syncytrotrophoblast cells increased significantly in the CVD group, while the average number of fibrinoid necrosis showed no significant changes. The mRNA levels of CD31, D2-40, FLT-1, PlGF, and HIF-1α in the CVD group also increased significantly, and the proportion of villi cells positive of BAX, caspase-3 and caspase-9 rose significantly, while that of villi cells positive positive of Bcl2 showed no obvious change. CVD group also reported a marked increase in the number of blood vessels positive of CD31, D2-40, FLT-1 and PlGF. The expression level of HIF-1α in syncytiotrophoblast cells, cytotrophoblast cells and fetal capillary villi increased significantly in the CVD group. The percentage of placenta with PAS positive substances observed an increase in the villi, in comparison to the control group. Conclusion The production of blood vessels and lymph vessels increased in the placental villi of patients with CVD, coupled with an accelerated apoptosis of villi cells. Meanwhile, CVD patients show an impaired function of placental villi and obstructed gas exchange between mother and infant.
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June 2021

Nanopore/Illumina hybrid genome sequence resource for strain XJ infecting rubber tree in China.

Plant Dis 2021 May 21. Epub 2021 May 21.

Henan Agricultural University, 70573, Department of Plant Pathology , Zhengzhou, Henan, China;

is a ubiquitous pathogenic fungus that can infect a broad range of plant hosts. Corynespora leaf fall (CLF) caused by C. cassiicola is one of the major diseases on rubber tree in China. This disease is having an increasing affect on natural rubber production worldwide. In this study, by combining the Nanopore and Illumina sequencing technologies, we present the chromosome-scale genome sequence of the rubber tree-sampled C. cassiicola strain XJ collected in the sub-tropical region of China. The assembly consists of 23 scaffolds (N50, 4.62 Mb) with an estimated genome size of 44.42 Mb (only 166 non-ATCG bases) and 16,108 protein-coding genes. The genome will provide a valuable resource for further research on the pathogenesis and comparative genomics of C. cassiicola on rubber tree and other hosts.
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http://dx.doi.org/10.1094/PDIS-03-21-0458-ADOI Listing
May 2021

The effect of Omaha System-based continuous nursing care on the psychological status, self-esteem, and quality of life in epileptic children.

Am J Transl Res 2021 15;13(4):3435-3442. Epub 2021 Apr 15.

Department of Nursing, Haikou Hospital Affiliated to Xiangya Medical College of Central South University Haikou 570208, Hainan, China.

Objectives: This study explored and analyzed the effect of Omaha System-based continual nursing care on the psychological status, self-esteem and quality of life in epileptic children.

Methods: 127 epileptic children hospitalized from March 2018 to September 2019 were recruited as the study cohort and stochastically divided into an observation group (n=65) and a control group (n=62). The control group children were given regular out-of-hospital guidance after discharge, and the observation-group were treated with Omaha System-based continuous nursing intervention in addition to the routine out-of-hospital guidance the control group underwent. The two groups' psychological statuses, self-esteem, sense of defect, and quality of life were compared both pre- and post-intervention.

Results: The SAS and SDS scores in the two groups after the intervention were significantly lower than their pre-intervention scores (P<0.05), and the scores in the observation group after the intervention were evidently lower than the scores in the control group (P<0.05). The two groups' post-intervention SES and FIS scores were significantly higher than their pre-intervention scores (P<0.05), and the scores in the observation group were notably lower than the scores in the control group (P<0.05). The post-intervention quality of life scores in the two groups were dramatically higher than the pre-intervention scores (P<0.05), and the quality of life scores in the observation group were notably higher than the quality of life scores in the control group (P<0.05).

Conclusion: The Omaha System-based continual nursing care can efficaciously elevate the psychological status of children with epilepsy, improve their self-esteem, reduce their sense of self-defect, and contribute to the improvement of their quality of life, so it is worthy of clinical promotion and application.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129271PMC
April 2021

Progress in the Clinical Features and Pathogenesis of Abnormal Liver Enzymes in Coronavirus Disease 2019.

J Clin Transl Hepatol 2021 Apr 25;9(2):239-246. Epub 2021 Apr 25.

Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

With the rapid development of research on coronavirus disease 2019 (COVID-19), more and more attention has been drawn to its damage to extrapulmonary organs. There are increasing lines of evidence showing that liver injury is closely related to the severity of COVID-19, which may have an adverse impact on the progression and prognosis of the patients. What is more, severe acute respiratory syndrome coronavirus-2 infection, cytokine storm, ischemia/hypoxia reperfusion injury, aggravation of the primary liver disease and drug-induced liver injury may all contribute to the hepatic damage in COVID-19 patients; although, the drug-induced liver injury, especially idiosyncratic drug-induced liver injury, requires further causality confirmation by the updated Roussel Uclaf Causality Assessment Method published in 2016. Up to now, there is no specific regimen for COVID-19, and COVID-19-related liver injury is mainly controlled by symptomatic and supportive treatment. Here, we review the clinical features of abnormal liver enzymes in COVID-19 and pathogenesis of COVID-19-related liver injury based on the current evidence, which may provide help for clinicians and researchers in exploring the pathogenesis and developing treatment strategies.
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http://dx.doi.org/10.14218/JCTH.2020.00126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111107PMC
April 2021

Gestational high-fat diet impaired demethylation of Pparα and induced obesity of offspring.

J Cell Mol Med 2021 06 6;25(12):5404-5416. Epub 2021 May 6.

The Key Laboratory of Reproductive Genetics (Zhejiang University School of Medicine), Ministry of Education, Hangzhou, China.

Gestational and postpartum high-fat diets (HFDs) have been implicated as causes of obesity in offspring in later life. The present study aimed to investigate the effects of gestational and/or postpartum HFD on obesity in offspring. We established a mouse model of HFD exposure that included gestation, lactation and post-weaning periods. We found that gestation was the most sensitive period, as the administration of a HFD impaired lipid metabolism, especially fatty acid oxidation in both foetal and adult mice, and caused obesity in offspring. Mechanistically, the DNA hypermethylation level of the nuclear receptor, peroxisome proliferator-activated receptor-α (Pparα), and the decreased mRNA levels of ten-eleven translocation 1 (Tet1) and/or ten-eleven translocation 2 (Tet2) were detected in the livers of foetal and adult offspring from mothers given a HFD during gestation, which was also associated with low Pparα expression in hepatic cells. We speculated that the hypermethylation of Pparα resulted from the decreased Tet1/2 expression in mothers given a HFD during gestation, thereby causing lipid metabolism disorders and obesity. In conclusion, this study demonstrates that a HFD during gestation exerts long-term effects on the health of offspring via the DNA demethylation of Pparα, thereby highlighting the importance of the gestational period in regulating epigenetic mechanisms involved in metabolism.
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http://dx.doi.org/10.1111/jcmm.16551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184666PMC
June 2021

Phenalen-1-one-mediated photodynamic therapy inhibits keloid graft progression by reducing vessel formation and promoting fibroblast apoptosis.

Adv Clin Exp Med 2021 Apr;30(4):431-439

Department of Burn Orthopedics, Xingtai People's Hospital, China.

Background: Keloid is a unique refractory syndrome characterized by a proliferation disorder of the fibroblasts. Recently, photodynamic therapy (PDT) has become a promising technique to modulate fibroblasts. However, use of the photosensitizer Phenalen-1-one (Ph1) in PDT for keloid remains to be explored.

Objectives: This study investigated the efficacy of Ph1-PDT in the in vitro and in vivo models of keloid.

Material And Methods: Cell viability was assessed with a Cell Counting Kit-8 (CCK-8) analysis in keloid fibroblasts. The migrated and invaded keloid fibroblasts after Ph1-PDT were detected using scratch and matrigel invasion assays in vitro. Flow cytometry measured the apoptosis changes. The protein concentrations and the mRNA expression of inflammatory modulators (interleukin 8 (IL-8) and IL-1β) were determined using enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase chain reaction (RT-qPCR) methods, respectively. Nude mice were used to perform the transplantation of keloid grafts. Western blot analysis measured the protein expression of CD31, CD34, tumor growth factor β1 (TGF-β1), and collagen 1 in keloid fibroblasts and grafts.

Results: Our results revealed that Ph1-PDT significantly suppressed cell viability, migration and invasion, and enhanced the rate of cell apoptosis and caspase-3 expression in keloid fibroblasts. Moreover, in the nude mice model, Ph1-PDT decreased the volume of the graft and attenuated the vessel density by inhibiting the expression of vessel density biomarkers (CD31 and CD34) in keloid grafts. Furthermore, Ph1-PDT significantly inactivated the inflammatory mediators in keloid grafts. In addition, Ph1-PDT considerably attenuated the development of keloids by inhibiting TGF-β1 and collagen 1 proteins in keloid fibroblasts and grafts.

Conclusions: Ph1-PDT may suppress keloid progression by reducing vessel formation and inflammation, and promoting fibroblast apoptosis, suggesting a potential therapy method for keloid.
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http://dx.doi.org/10.17219/acem/130594DOI Listing
April 2021

Synthesis and luminescence properties of a broadband near-infrared emitting non-gallate persistent luminescence MgZnSnO:Cr phosphor.

Dalton Trans 2021 Apr;50(16):5666-5675

State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. and The GBA National Institute for Nanotechnology Innovation, Guangzhou, 510535, China.

A series of novel non-gallate near-infrared long-persistent phosphorescence Mg2-xZnxSnO4:Cr3+ phosphors were synthesized, and their structure and luminescence properties were investigated systematically. Under 448 nm blue light excitation, all the phosphors exhibit a broad emission band centered at 730 nm and a shoulder peak at 708 nm, which are attributed to the 4T2(4F) → 4A2 and 2E → 4A2 transitions of Cr3+, respectively. The excitation spectra of the samples clearly show the characteristic excitation of Cr3+ in the octahedral crystal field, with three obvious peaks at 324, 448 and 620 nm respectively. The phosphor with a composition of Mg1.4Zn0.6SnO4:0.03Cr3+ shows the strongest photoluminescence intensity which is 2.87 times and 3.09 times that of Mg2SnO4:0.03Cr3+ and Zn2SnO4:0.03Cr3+, respectively. Besides, all the samples show intense near-infrared long-persistent phosphorescence. For the optimized sample Mg1.4Zn0.6SnO4:0.005Cr3+, its phosphorescence can still be observed with a night vision instrument 18 h after removing the 365 nm UV light source. Finally, a feasible phosphorescence mechanism of the Mg1.4Zn0.6SnO4:Cr3+ phosphor was proposed and discussed. This study may provide a new method for developing novel near-infrared long-persistent phosphorescence phosphors through crystal structure modification.
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http://dx.doi.org/10.1039/d1dt00452bDOI Listing
April 2021

Uptake, Tissue Distribution, and Elimination of 8:2 Polyfluoroalkyl Phosphate Diesters in Mytilus galloprovincialis.

Environ Toxicol Chem 2021 Jul 28;40(7):1992-2004. Epub 2021 May 28.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.

Although the distribution of 8:2 polyfluoroalkyl phosphate diester (8:2 diPAP) in aquatic environments has been reported, details on its uptake, tissue specificity, and elimination in bivalve mollusks remain to be clarified. The present study is the first report on the accumulation and elimination of 8:2 diPAP in mussels (Mytilus galloprovincialis). The tissue-specific accumulation and depuration of 8:2 diPAP and its metabolites were investigated via semistatic seawater exposure (8:2 diPAP at a nominal concentration of 10 μg/L), through water-borne exposure with static daily renewal over a 72-h exposure period and a 360-h depuration period. The digestive gland was found to be the target organ where accumulation and biotransformation primarily occur. The bioaccumulation factor values (mL/g dry wt) in different organs were in the following order: digestive gland (1249) > adductor muscle (315) > gills (289) > gonad (82.9) > mantle (33.0). Moreover, the distribution of 8:2 diPAP among tissues may be related to the total protein content. The 8:2 diPAP tended to be excreted in feces. The compounds 8:2 fluorotelomer carboxylic acid, 8:2 fluorotelomer unsaturated carboxylic acid, 7:3 fluorotelomer carboxylic acid, perfluorooctanoic acid, and perfluoroheptanoic acid were detected and quantified as phase I metabolites, and the concentration of all phase I metabolites relative to the 8:2 diPAP concentration (72 h) was 0.304 mol%. A phase II metabolite, 8:2 fluorotelomer alcohol conjugated with sulfate, was detected but not quantitated in the digestive gland. A biotransformation pathway of 8:2 diPAP in M. galloprovincialis was proposed on the basis of the results obtained in the present study and previous studies. These findings improve our understanding of the accumulation of perfluorocarboxylic acids in bivalve mollusks. Environ Toxicol Chem 2021;40:1992-2004. © 2021 SETAC.
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http://dx.doi.org/10.1002/etc.5060DOI Listing
July 2021

Preliminary Assessment of the Resource and Exploitation Potential of Lower Permian Marine-Continent Transitional Facies Shale Gas in the Huainan Basin, Eastern China, Based on a Comprehensive Understanding of Geological Conditions.

ACS Omega 2021 Mar 22;6(12):8502-8516. Epub 2021 Mar 22.

School of Earth and Environment, Anhui University of Science & Technology, Huainan 232001, China.

The Huainan Basin in eastern China contains abundant shale gas resources; the Lower Permian is an exploration horizon with a high potential for shale gas in marine-continent transitional facies. However, few detailed analyses have investigated shale gas in this area. In this paper, a comprehensive investigation of the geochemical characteristics, physical properties, and gas-bearing capacities of shale reservoirs was conducted, and the resource and exploitation potential were evaluated. The results show that the cumulative thicknesses of the Shanxi Formation (P) and lower Shihezi Formation (P) are mostly greater than 35 and 65 m, respectively. The TOC contents of the P and P shale vary from 0.11 to 8.87% and from 0.22 to 14.63%, respectively; the kerogens predominantly belong to type II with minor amounts of type I or type III kerogens; average values range between 0.83 and 0.94% and between 0.82 and 1.02% in P and P, respectively; the shale samples are primarily at a low maturity, while some shale samples have entered the high-maturity stage. The shale reservoirs have low permeability and porosity in P and P, respectively. The pores of the P shale reservoir are characterized by well-developed micropores and transition pores and poorly developed mesopores, while the pores in the P shale reservoir are all characterized by well-developed micropores and transition pores and some well-developed macropores; the different pore types in the shale reservoirs developed in the organic matter, clay minerals, and pyrite, while a few endogenous fractures developed in the organic matter and structural fractures developed in the minerals. The total shale gas contents in P and P are 2.85 and 2.96 m t, respectively. The P shale reservoir has a higher hydrocarbon generation potential than P and has a lower gas generation potential. The total shale gas amounts in P and P are 3602.29-4083.04 × 10 and 2811.04-3450.77 × 10 m, respectively. Further research on shale gas exploration and exploitation for these formations needs to be performed.
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http://dx.doi.org/10.1021/acsomega.1c00290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015131PMC
March 2021

Serum kisspeptin levels in polycystic ovary syndrome: A meta-analysis.

J Obstet Gynaecol Res 2021 Jun 25;47(6):2157-2165. Epub 2021 Mar 25.

Department of Pharmacology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.

Aim: To clarify the association of serum kisspeptin levels in women with polycystic ovary syndrome (PCOS) by meta-analysis.

Methods: Two English databases and two Chinese databases were searched for the relationship between kisspeptin and PCOS published from 2009. After the studies screening according to specific principles, we used STATA 12.0 for meta-analysis. Standardized mean difference (SMD) and its 95% confidence intervals (95% CIs) were used as the effect size and STATA 12.0 software was performed by this meta-analysis.

Results: Nine articles were included in the end, with a total of 1282 participants (699 patients and 583 controls). Heterogeneity between studies was statistically significant. Therefore, the random effects model was used to combine the effects. Meta-analysis showed statistically significant differences in serum kisspeptin levels between the PCOS patients and controls (SMD = 0.57, 95% CI [0.32, 0.82]), which indicated that there is a strong association between serum kisspeptin levels and PCOS. The source of high heterogeneity between the inclusion studies (I  = 73.2%) might be due to the small sample size. The larger variation of kisspeptin concentration might be caused by different diagnosis criteria of PCOS and short half-time period of kisspeptin combined with nonstandard testing process.

Conclusion: Serum kisspeptin levels in PCOS patients were higher than non-PCOS patients. It is a hint to indicate us that kisspeptin might be an independent biomarker of PCOS patients.
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http://dx.doi.org/10.1111/jog.14767DOI Listing
June 2021

Anatomical Findings and Short-term Efficacy of Fascial Anatomy-guided Infrapyloric Lymphadenectomy in Laparoscopic Radical Gastrectomy for Gastric Cancer.

Surg Laparosc Endosc Percutan Tech 2021 Mar 12;31(4):434-438. Epub 2021 Mar 12.

Gastrointestinal Surgery Unit 1, Teaching Hospital of Putian First Hospital of Fujian Medical University.

Objective: The aim was to investigate the anatomical layers of the specific fascia involved in infrapyloric lymphadenectomy in laparoscopic radical gastrectomy for gastric cancer and to analyze the short-term efficacy of an anatomy-guided surgical approach.

Materials And Methods: On the basis of many years of clinical practice in fascial anatomy-guided laparoscopic radical gastrectomy for gastric cancer, we proposed anatomical considerations for infrapyloric lymphadenectomy in this procedure and investigated the anatomy of the mesentery and mesenteric fusion in this region, including the specific starting and ending points and the plane of the operation. We also retrospectively analyzed the clinical data of 265 patients who underwent fascial anatomy-guided infrapyloric lymphadenectomy in laparoscopic radical gastrectomy for gastric cancer from January 2015 to January 2019 and compared the short-term efficacy between the fascial anatomy-guided laparoscopic infrapyloric lymphadenectomy plus mesogastric excision group and the laparoscopic infrapyloric lymphadenectomy group.

Results: Extensive mesenteric fusion and folds exist in the infrapyloric region of the stomach, and removal of the medial fold (medial leg) and lateral fold (lateral leg) of the infrapyloric mesogastrium during surgery is easily missed, resulting in incomplete removal of the infrapyloric mesogastrium. Baseline data were comparable between the laparoscopic infrapyloric lymphadenectomy plus mesogastric excision group and the laparoscopic infrapyloric lymphadenectomy group. The mean operative time for infrapyloric lymphadenectomy, the number of positive lymph nodes harvested in the infrapyloric region, and the number of patients with mesenteric metastasis in the infrapyloric region were not significantly different (P>0.05). The number of harvested lymph nodes was higher in the laparoscopic infrapyloric lymphadenectomy plus mesogastric excision group than in the laparoscopic infrapyloric lymphadenectomy group (5.09±3.30 vs. 4.13±2.90, P<0.05), and intraoperative blood loss was lower in the former group than in the latter group (5.89±3.78 vs. 25.21±11.24 mL, P=0.000).

Conclusion: Fascial anatomy-guided laparoscopic infrapyloric lymphadenectomy enables systematic and complete removal of the lymph nodes and mesentery of the infrapyloric region with less intraoperative blood loss.
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http://dx.doi.org/10.1097/SLE.0000000000000886DOI Listing
March 2021

Seven computations of the social brain.

Soc Cogn Affect Neurosci 2021 Aug;16(8):745-760

Department of Humanities and Social Sciences, California Institute of Technology, Pasadena, CA 91125, USA.

The social environment presents the human brain with the most complex information processing demands. The computations that the brain must perform occur in parallel, combine social and nonsocial cues, produce verbal and nonverbal signals and involve multiple cognitive systems, including memory, attention, emotion and learning. This occurs dynamically and at timescales ranging from milliseconds to years. Here, we propose that during social interactions, seven core operations interact to underwrite coherent social functioning; these operations accumulate evidence efficiently-from multiple modalities-when inferring what to do next. We deconstruct the social brain and outline the key components entailed for successful human-social interaction. These include (i) social perception; (ii) social inferences, such as mentalizing; (iii) social learning; (iv) social signaling through verbal and nonverbal cues; (v) social drives (e.g. how to increase one's status); (vi) determining the social identity of agents, including oneself and (vii) minimizing uncertainty within the current social context by integrating sensory signals and inferences. We argue that while it is important to examine these distinct aspects of social inference, to understand the true nature of the human social brain, we must also explain how the brain integrates information from the social world.
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http://dx.doi.org/10.1093/scan/nsab024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343565PMC
August 2021

Association between NER pathway gene polymorphisms and neuroblastoma risk in an eastern Chinese population.

Mol Ther Oncolytics 2021 Mar 19;20:3-11. Epub 2020 Dec 19.

Department of Pathology, Children's Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu, China.

Neuroblastoma is a common childhood malignancy. Nucleotide excision repair (NER) polymorphisms have been shown to influence cancer susceptibility by modifying DNA repair efficiency. To investigate the association of NER gene polymorphisms with neuroblastoma risk, we constructed a three-center case-control study. A total of 19 candidate single-nucleotide polymorphisms (SNPs) in NER genes were analyzed. Odds ratios (ORs) and 95% confidential intervals (CIs) were calculated to evaluate the associations. We identified five independent SNPs that were significantly associated with neuroblastoma risk, including rs1800975 (dominant model: adjusted OR = 0.73, 95% CI = 0.55-0.98, p = 0.033), rs3176752 (recessive model: adjusted OR = 2.78, 95% CI = 1.12-6.91, p = 0.028), rs3810366 (dominant: adjusted OR = 1.44, 95% CI = 1.05-1.97, p = 0.022; recessive: adjusted OR = 1.58, 95% CI = 1.18-2.11, p = 0.002), rs238406 (dominant: adjusted OR = 0.64, 95% CI = 0.48-0.84, p = 0.002; recessive: adjusted OR = 0.67, 95% CI = 0.48-0.94, p = 0.021), and rs2094258 (recessive: adjusted OR = 1.44, 95% CI = 1.03-2.04, p = 0.036). Stratified analysis was carried out. Furthermore, these findings were strengthened by false-positive report probability (FPRP) analysis and expression quantitative trait loci (eQTL) analysis. In conclusion, our study indicates that five SNPs in NER genes are correlated with neuroblastoma susceptibility in the eastern Chinese population, providing novel insight into the genetic underpinnings of neuroblastoma. However, further large-scale studies are required to verify these findings.
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http://dx.doi.org/10.1016/j.omto.2020.12.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851491PMC
March 2021

Deep Targeted Sequencing and Its Potential Implication for Cancer Therapy in Chinese Patients with Gastric Adenocarcinoma.

Oncologist 2021 05 8;26(5):e756-e768. Epub 2021 Feb 8.

Department of Gastric Surgery, Institute of Cancer Research and Basic Medical Sciences of Chinese Academy of Sciences, Cancer Hospital of University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China.

Introduction: Gastric cancer (GC) has a high incidence and mortality rate, especially in East Asians, and about 90% of GCs are adenocarcinomas. Histological and etiological heterogeneity and ethnic diversity make molecular subtyping of GC complicated, thus making it difficult to determine molecular division systems and standard treatment modalities. Limited cohorts from South Korea, Singapore, Australia, and Japan have been studied; however, the mutational landscape of gastric adenocarcinomas in Chinese patients is still unknown.

Methods: We performed a targeted sequencing panel focusing on cancer-related genes and tumor-associated microorganisms of 529 gastric adenocarcinoma samples with matched blood controls. We identified 449 clinically relevant gene mutations.

Results: Approximately 47.1% of Chinese patients with GC harbored at least one actionable mutation. The top somatic mutations were TP53, ARID1A, LRP1B, PIK3CA, ERBB2, CDH1, KRAS, FAT4, CCNE1, and KMT2D. Truncation mutations of ARID1A, KMT2D, RNF43, TGFBR2, and CIC occurred in patients with high tumor mutational burden. Gene amplifications of ERBB2, CCNE1, CDK12, and CCND1 were detected in patients with low tumor mutational burden. Pathway analysis revealed common gene alterations in the Wnt and PI3K/Akt signaling pathways. The ratio of patients with high microsatellite instability was significantly lower than other cohorts, and high microsatellite instability and Epstein-Barr virus (EBV)-positive features seemed mutually inclusive in Chinese patients with GC. In 44 (8.3%) patients, 45 germline mutations were identified, among which SPINK1 mutations, all SPINK1 c.194 + 2T > C, were present in 15.9% (7/44) of patients. Microorganisms found in Chinese patients with GC included Helicobacter pylori, EBV, hepatitis B virus, and human papillomavirus types 16 and 18.

Conclusion: Identification of varied molecular features by targeted next-generation sequencing provides more insight into patient stratification and offers more possibilities for both targeted therapies and immunotherapies of Chinese patients with GC.

Implications For Practice: This study investigated the genomic alteration profile of 529 Chinese patients with gastric adenocarcinoma by deep targeting sequencing, which might be the largest Chinese cohort on the genomic research of gastric adenocarcinoma up to now.
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http://dx.doi.org/10.1002/onco.13695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100567PMC
May 2021

FeO nanoparticles as matrix solid-phase dispersion extraction adsorbents for the analysis of thirty pesticides in vegetables by ultrahigh-performance liquid chromatography-tandem mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Feb 9;1165:122532. Epub 2021 Jan 9.

Guangxi Key Laboratory of Agro-Environment and Agro-Product Safety, Agricultural College, Guangxi University, Nanning 530005, PR China. Electronic address:

Herein we report the first example of FeO nanoparticles (FNPs) being used as single-matrix solid-phase dispersion (MSPD) adsorbents for the extraction of 30 representative pesticides from vegetables. This study was aimed at analyzing the extracted samples using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Various condition parameters, such as the eluent, volume of the eluent, and amount of FNPs were optimized to achieve good sensitivity and precision for the elution and extraction of the analytes. The developed method was validated using matrices consisting of eight vegetables (lettuce, cucumber, carrot, tomato, pepper, shallot, Chinese flowering cabbage, and cabbage) spiked with 30 pesticides at concentrations of 0.01, 0.1, and 1.0 mg/kg. The recoveries of the 30 pesticides (organophosphorus, triazole, carbamate, nicotine, amide, and other different structures of pesticides) were in the range 71.0-110.8% (n = 5) (except those of prothioconazole and dinotefuran), with relative standard deviations lower than 13.5% in all the matrices under optimal conditions. The matrix effects were observed by comparing the slope of the matrix-matched standard calibration curve with that of the solvent. However, the matrix effects of the eight vegetables did not show evident regularities. For pepper, tomato, and shallot, a sizable number of pesticides (24, 21, and 21, respectively) showed suppressive matrix effects. On the other hand, for cucumber, Chinese flowering cabbage, and cabbage, a good number of pesticides (19, 18, and 15, respectively) showed negligible matrix effects. Furthermore, for carrot matrices, 21 pesticides showed a matrix enhancement effect. Excellent linearity was achieved at pesticide concentrations of 0.01-1.0 mg/L, and the limits of quantification (LOQ) for the developed method reached 0.01 mg/kg (except that for dinotefuran, which was 0.1 mg/kg), based on the spiked test. The developed method was successfully employed in the analysis of real samples in Nanning, China, and three pesticide residues (halosulfuron methyl, tebuconazole, and azoxystrobin) were commonly detected in vegetable samples. In the present study, a reliable method-validation performance and excellent cleanup effects were observed by using the modified MSPD method consisting of the FNPs in the cleanup step.
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http://dx.doi.org/10.1016/j.jchromb.2021.122532DOI Listing
February 2021

Graph Theoretic Analysis Reveals Intranasal Oxytocin Induced Network Changes Over Frontal Regions.

Neuroscience 2021 04 21;459:153-165. Epub 2021 Jan 21.

Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China. Electronic address:

In this study, we aim to elucidate how intranasal oxytocin modulates brain network characteristics, especially over the frontal network. As an essential brain hub of social cognition and emotion regulation, we also explore the association between graphic properties of the frontal network and individual personality traits under oxytocin (OT) administration. Fifty-nine male participants administered intranasal OT or placebo were followed by resting-state fMRI scanning. The correlation-based network model was applied to study OT modulation effects. We performed community detection algorithms and conducted further network analyses, including clustering coefficient, average shortest path and eigenvector centrality. In addition, we conducted a correlation analysis between clustering coefficients and the self-assessed psychological scales. Modular organizations in the OT group reveal integrations of the frontoparietal network (FPN) and the default mode network (DMN) over frontal regions. Results show that frontal nodes within the FPN are characterized by lower clustering coefficients and higher average shortest path values compared to the placebo group. Notably, these modulation effects on frontal network property are associated with Interpersonal Reactivity Index (IRI) fantasy value. Our results suggest that OT elevates integrations between FPN, DMN and limbic system as well as reduces small-worldness within the FPN. Our results support graph theoretic analysis as a potential tool to assess OT induced effects on the information integration in the frontal network.
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http://dx.doi.org/10.1016/j.neuroscience.2021.01.018DOI Listing
April 2021

Case Report of a pathologically confirmed vascular parkinsonism with early cognitive impairment and Behavioral disturbance.

BMC Neurol 2021 Jan 11;21(1):15. Epub 2021 Jan 11.

Institute of Systems Biomedicine, Peking University Health Science Center, Beijing, 100191, P.R. China.

Background: Vascular Parkinsonism(VaP) is defined as parkinsonism resulting from cerebral vascular disease(CVD), with presence of variable motor and non-motor signs that are corroborated by clinical, anatomic or imaging findings of cerebrovascular disease. Overlapping syndromes with mixed pathologies make VaP difficult to distinguish from primary neurodegenerative parkinsonism.To understand the clinical and pathological features of VaP,we report a case of autopsy confirmed vascular Parkinsonism that was clinical misdiagnosed as idiopathic Parkinson's disease.Clinical features include early mixed symptoms of dementia,behavioral disturbance and parkinsonism that were similar to Dementia with lewy Body(DLB) and Parkinson disease Dementia(PDD).

Case Presentation: A 84-year-old man presented progressive parkinsonism with prominent postural instability, gait impairment, pseudobulbar, early cognitive impairment, irritability, hallucination, urinary symptoms and poor responsiveness to dopaminergic drugs. He was clinically diagnosed as Parkinson disease(PD). In the post-mortem study, we examined Aβ and phospho-tau as pathological biomarker for Alzheimer's disease(AD), α-synucleing in medulla, pons and midbrain for PD and DLB. Hematoxylin and eosin staining in cerebral cortex, cerebellum and brainstem examines vascular pathological changes and microvascular lesion.Neither Lewy bodies in the substantia nigra ,locus ceruleus and cerebrumnor accumulation of Aβ, neurofibrillary tangles were noted. Instead, there were many cerebral infarctions and widespread arteriosclerosis in the brain. The final brain autopsy supported a diagnosis of VaP not PD.

Conclusions: This case of pathologically confirmed VaP misdiagnosed as idiopathic PD suggested that we must be vigilant about the possibility of VaP for patients with parkinsonisms, cognitive impairments, early behavioral and psychological symptoms,imaging performances of cerebral small vessel disease and other vascular damages.
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http://dx.doi.org/10.1186/s12883-020-02038-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798293PMC
January 2021

MicroRNA-30a-5p silencing polarizes macrophages toward M2 phenotype to alleviate cardiac injury following viral myocarditis by targeting SOCS1.

Am J Physiol Heart Circ Physiol 2021 04 8;320(4):H1348-H1360. Epub 2021 Jan 8.

Department of Cardiology, the First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, People's Republic of China.

Viral myocarditis (VMC) is a life-threatening disease characterized by severe cardiac inflammation generally caused by coxsackievirus B3 (CVB3) infection. Several microRNAs (miRNAs or miRs) are known to play crucial roles in the pathogenesis of VMC. The study aimed to decipher the role of miR-30a-5p in the underlying mechanisms of VMC pathogenesis. We first quantified miR-30a-5p expression in a CVB3-induced mouse VMC model. The physiological characteristics of mouse cardiac tissues were then detected by hematoxylin and eosin (HE) and Picrosirius red staining. We established the correlation between miR-30a-5p and SOCS1, using dual-luciferase gene assay and Pearson's correlation coefficient. The expression of inflammatory factors (IFN-γ, IL-6, IL-10, and IL-13), M1 polarization markers [TNF-α, inducible nitric oxide synthase (iNOS)], M2 polarization markers (Arg-1, IL-10), and myocardial hypertrophy markers [atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP)] was detected by RT-qPCR and Western blot analysis. miR-30a-5p was found to be highly expressed in VMC mice. Silencing of miR-30a-5p improved the cardiac function index and reduced heart weight-to-body weight ratio, myocardial tissue pathological changes and fibrosis degree, serological indexes, as well as proinflammatory factor levels, while enhancing anti-inflammatory factor levels in VMC mice. Furthermore, silencing of miR-30a-5p inhibited M1 polarization of macrophages while promoting M2 polarization in vivo and in vitro. SOCS1 was a target gene of miR-30a-5p, and the aforementioned cardioprotective effects of miR-30a-5p silencing were reversed upon silencing of SOCS1. Overall, this study shows that silencing of miR-30a-5p may promote M2 polarization of macrophages and improve cardiac injury following VMC via SOCS1 upregulation, constituting a potential therapeutic target for VMC treatment. We found in this study that microRNA (miR)-30a-5p inhibition might improve cardiac injury following viral myocarditis (VMC) by accelerating M2 polarization of macrophages via SOCS1 upregulation. Furthermore, the anti-inflammatory mechanisms of miR-30a-5p inhibition may contribute to the development of new therapeutic strategies for VMC.
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http://dx.doi.org/10.1152/ajpheart.00431.2020DOI Listing
April 2021

Modality-specific neural mechanisms of cognitive control in a Stroop-like task.

Brain Cogn 2021 02 21;147:105662. Epub 2020 Dec 21.

CAS Key Laboratory of Behavioral Science, Institute of Psychology, Beijing, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

The successful resolution of ever-changing conflicting contexts requires efficient cognitive control. Previous studies have found similar neural patterns in conflict processing for different modalities using an event-related potential (ERP) approach and have concluded that cognitive control is supramodal. However, recent behavioral studies have found that conflict adaptation (a phenomenon with the reduction of congruency effect in the current trial after an incongruent trial as compared with a congruent trial) could not transfer across visual and auditory modalities and suggested that cognitive control is modality-specific, challenging the supramodal view. These discrepancies may have also arisen from methodological differences across studies. The current study examined the electroencephalographic profiles of a Stroop-like task to elucidate the modality-specific neural mechanisms of cognitive control. Participants were instructed to respond to a target always coming from the visual modality while disregarding the distractor coming from either the auditory or the visual modality. The results revealed significant congruency effects on both behavioral indices, i.e., reaction time and error rate, and ERP components, including the P3 and the conflict slow potential. Besides, the congruency effects on the amplitude of the P3 showed a negative correlation with reaction time, indicating an intrinsic link between these neural and behavioral indices. Furthermore, in the modality-repetition condition, conflict adaptation effects were significant on both reaction time and P3 amplitude, and the reaction time could be predicted by the P3 amplitude, while such effects were not observed in the modality-alternation condition. The time-frequency analysis also showed that conflict adaptation occurred in the modality-repetition condition, but not in the modality-alternation condition in low frequency bands, including the theta (4-8 Hz), alpha (8-12 Hz), and beta1 (12-20 Hz) bands. Taken together, our results revealed modality-specific patterns of the conflict adaptation effects on the P3 amplitude and oscillatory power (in theta, alpha, and beta1 bands), providing neural evidence for the modality specificity of cognitive control and expanding the boundaries of cognitive control.
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http://dx.doi.org/10.1016/j.bandc.2020.105662DOI Listing
February 2021
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