Publications by authors named "Haiyan Wang"

1,462 Publications

  • Page 1 of 1

Insulin-like growth factor binding protein 5 accelerate the senescence of periodontal ligament stem cells.

Cell Tissue Bank 2022 Aug 8. Epub 2022 Aug 8.

Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, People's Republic of China.

Evidences have showed stem cell mediated tissue regeneration is a promising method for the treatment of periodontitis. Insulin-like growth factor binding proteins-5 (IGFBP5) is a member of the insulin growth factor (IGFs) family and plays a regulatory role in cell proliferation and differentiation. Our previous study showed that IGFBP5 can promote osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and enhance periodontal tissue regeneration mediated by PDLSCs. However, the function of IGFBP5 in the process of PDLSCs senescence remains unclear. The present study showed IGFBP5 mRNA level was highly expressed in passage-induced aged PDLSCs cells. IGFBP5 knockdown decreased the ratio of senescence associated β-galactosidase (SA-β-Gal) positive cells, enhanced the activity of TERT, and down-regulated the expression levels of P16, P21, P53 mRNA and protein. Overexpression of IGFBP5 increased the ratio of SA-β-Gal positive staining PDLSCs, decreased the activity of telomerase TERT, and up-regulated the expression levels of P16, P21, P53 mRNA and protein related to PDLSCs senescence. In conclusion, IGFBP5 can accelerate the senescence of PDLSCs, indicating the potential target for maintaining the "young state" of stem cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10561-022-10030-8DOI Listing
August 2022

An online tool for predicting ovarian reserve based on AMH level and age: A retrospective cohort study.

Front Endocrinol (Lausanne) 2022 22;13:946123. Epub 2022 Jul 22.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.

Purpose: To establish a more convenient ovarian reserve model with anti-Müllerian hormone (AMH) level and age (the AA model), with blood samples taken at any time in the menstrual cycle.

Methods: We have established this AA model for predicting ovarian reserve using the AMH level and age. The outcome variable was defined as poor ovarian response (POR) with <5 oocytes retrieved during assisted reproductive technology treatment cycles. Least Absolute Shrinkage and Selection Operator logistic regression with 5-fold cross validation methods was applied to construct the model, and that with the lowest scaled log-likelihood was selected as the final one.

Results: The areas under the receiver operating characteristic curve for the training, inner, and external validation sets were 0.862, 0.843, and 0.854 respectively. The main effects of AMH level and age contributing to the prediction of POR were 95.3% and 1.8%, respectively. The incidences of POR increased with its predicted probability in both the model building and in external validation datasets, indicating its stability. An online website-based tool for assessing the score of ovarian reserve (http://121.43.113.123:9999) has been developed.

Conclusions: Based on external validation data, the AA model performed well in predicting POR, and was more cost-effective and convenient than our previous published models.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2022.946123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353219PMC
August 2022

Accounting Transparency, Fear Sentiment and the COVID-19 Epidemic: For Public Health Security and the Construction of an Early Warning System.

Front Public Health 2022 19;10:908430. Epub 2022 Jul 19.

Business School, Zhejiang Wanli University, Ningbo, China.

A central issue of public health security and the construction of an early warning system is to establish a set of responsibility-oriented incentives and restraint mechanisms. This is closely related to the accounting transparency of the institutional environment and the fear sentiment of the individual's predicament. This study analyses the relationship between accounting transparency, fear sentiment, and COVID-19 through a VAR model analysis. The results show a significant and negative relationship between accounting transparency and daily new COVID-19 patients. In particular, accounting transparency has a negative impact on the increase in the number of people infected with a two-period lag, while the three-period lag in the number of new epidemics has a negative impact on accounting information. Second, accounting transparency has a positive impact on the increase in the search volume on COVID-19 within a three-period lag. After the three-period lag, the number of new epidemics has a positive impact on accounting information. Third, an increase in fear sentiment can be driven by the fear of COVID-19. Fourth, in the public health early warning system, according to the abovementioned time characteristics, the system arranges the emotional counseling, early warning incentives, and institutional constraints to be dealt with in the first 4 days. In addition, in the early warning target-oriented system setting, the parallel system helps to improve the early warning efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2022.908430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347418PMC
August 2022

The Relationship Between Vitamin D and Activity of Daily Living in the Elderly.

Int J Gen Med 2022 1;15:6357-6364. Epub 2022 Aug 1.

Department of Nephrology, Shanghai Changhai Hospital, Shanghai, People's Republic of China.

Objective: To analyze the association between vitamin D and the performance of activity of daily living in the elderly.

Methods: A total of 94 patients over the age of 65 were eligible to participate if they had undergone a bone mineral density test and if they were in a stable health condition. Subjects were further divided into two groups according to activity of daily living (ADL): the score over 40 of the patients as the high ADL group and the below as the low ADL group.

Results: According to univariate analysis, the mean of total hip T score, serum creatinine/cystatin C ratio (CCR), and vitamin D were significantly different between the two groups (=0.024, 0.008, 0.010). Multivariate ORs showed that the CCR (OR: 0.948; 95%CI: 0.910-0.989; =0.013) and vitamin D (OR: 865; 95%CI: 0.752-0.994; =0.047) were inversely associated with having low ADL. Furthermore, on multiple linear regression analysis, the Barthel ADL index was related to geriatric nutritional risk index (GNRI), CCR and vitamin D but independent of patients' age with the slope of 0.732, 0.539, and 0.689 separately, reflected the stronger relative within the variables.

Conclusion: We demonstrated that there is a negative correlation of CCR and vitamin D with having low ADL in elderly population. Monitoring the trend of serum vitamin D and CCR, may have a role in the early detection of low ADL with loss of muscle mass and strength in the population of the elderly.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJGM.S366203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355018PMC
August 2022

In-depth profiling of urinary N-glycome in diabetic kidney disease by ultrafast glycoprotein immobilization for glycan extraction (UltraGIG).

Anal Chim Acta 2022 Aug 7;1221:340144. Epub 2022 Jul 7.

Department of Nephrology, Changhai Hospital, Second Military Medical University/Naval Medical University, Shanghai, 200433, PR China. Electronic address:

Diabetic kidney disease (DKD) is a common and serious kidney-related complication of diabetic mellitus. Although albuminuria and estimated glomerular filtration rate (eGFR) are commonly used diagnostic biomarkers, they have limitations in the diagnosis of DKD due to the lack of specificity and sensitivity. Urinary N-glycans are emerging as novel biomarkers for predicting renal prognosis in DKD. However, most of the current N-glycan profiling methods for DKD were based on lectin affinity enrichment or hydrophilic interaction chromatography (HILIC) with optical detection, which provided limited N-glycome coverage in low throughput. Herein, we developed a novel N-glycan purification method, termed Ultrafast Glycoprotein Immobilization for Glycan extraction (UltraGIG), for in-depth profiling of urinary N-glycome in DKD using mass spectrometry (MS). In UltraGIG, proteins were rapidly (within 40 min) immobilized to resin with high efficiency (over 98%) through NHS-reactive chemistry, and then N-glycans were enzymatically released from the resin by PNGase F. Owing to good efficiency of immobilization of proteins to resin, the subsequent washing steps to remove proteins and other impurities could be easily performed. The UltraGIG showed good selectivity towards glycans (extracting N-glycans from the mixture of IgG and BSA at a 1:100 M ratio) and samples loss was minimized (detection sensitivity at fmol level). A total of 237 N-glycan compositions were identified from urine samples with DKD and healthy controls, which representing the largest data set of N-glycome from DKD. Compared to healthy controls, 3 N-glycans were up-regulated and 14 N-glycans were down-regulated in DKD patients. Collectively, UltraGIG offers a competitive sample purification method for in-depth analysis of urinary N-glycome by MS and provide new insights into biomarker study for the diagnosis of DKD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2022.340144DOI Listing
August 2022

Activation of the JAK1/STAT1 signaling pathway is associated with peroxiredoxin 6 expression levels in human epididymis epithelial cells.

Folia Histochem Cytobiol 2022 Aug 5. Epub 2022 Aug 5.

Key Laboratory of Male Reproductive Health, National Health and Family Planning Commission, Beijing, PR China.

Introduction: Peroxiredoxin 6 (Prdx6) is widely expressed in mammalian tissues. Our previous study demonstrated that Prdx6 was expressed in human epididymis, present in human seminal fluid, and in spermatozoa. The protective role of Prdx6 in maintaining the viability and DNA integrity of human spermatozoa was also detected. Here, we demonstrate the potential role and mechanism of Prdx6 in human epididymis epithelial cells (HEECs).

Material And Methods: Western blotting was used to measure expression levels of key proteins in the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway. The malonaldehyde (MDA) levels and antioxidant capacity in HEECs were detected with the commercial kits. Digital gene expression analysis (DGE) was used to identify gene expression patterns in control and Prdx6-interference HEECs. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to validate the DGE findings.

Results: Compared to control HEECs, the expression levels of JAK1, STAT1, phosphorylated JAK1 and STAT1 were significantly increased, while the expression level of SOCS3 was significantly decreased in Prdx6-interference HEECs. The MDA level and total antioxidant capacity in Prdx6-interference HEECs were significantly increased and decreased compared to that of control, respectively. DGE analysis identified 589 up-regulated and 314 down-regulated genes (including Prdx6) in Prdx6-interference HEECs. Thirteen significantly different pathways were identified between the two groups, with the majority of genes belonging to the CCL, CXCL, IL, and IFIT family of proteins and were related to immunity. In particular, the expression levels of IL6, IL6ST, and eighteen IFN-related genes were significantly increased in Prdx6-interference HEECs compared to control HEECs.

Conclusions: We found that reduced Prdx6 expression induced higher ROS levels in HEECs, which resulted in the activation of the IL-6 receptor and IFNγ expression to induce the JAK1/STAT1 signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5603/FHC.a2022.0021DOI Listing
August 2022

Astragalus polysaccharide alleviates ulcerative colitis by regulating the balance of Tfh/Treg cells.

Int Immunopharmacol 2022 Aug 1;111:109108. Epub 2022 Aug 1.

Formula-Pattern Research Center, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China. Electronic address:

The immunomodulatory function of natural active ingredients has long been a focus of scientific research, with recent hotspots reporting targeted modulation of the follicular helper T cells (Tfh)/regulatory T cells (Treg) balance as an emerging strategy for the treatment of ulcerative colitis (UC). Here, dextran sodium sulfate induced mice UC and Astragalus polysaccharide (APS, 200 mg/kg/day) was administered simultaneously. In this study, APS effectively alleviated colitis in mice by improving survival rate, disease activity index (DAI), the change rate of body weight, colonic length and weight, and histopathological injury of the colon. Moreover, APS regulated the expression of inflammatory cytokines interleukin (IL)-2, IL-6, IL-12p70, IL-23, Tumour necrosis factor (TNF)-ɑ, and transforming growth factor (TGF)-β1 in colonic tissues of colitis mice. Importantly, APS significantly downregulated Tfh cell and the expression of its related nuclear transcription factors Blimp-1 and Bcl-6, and cytokine IL-21. Meanwhile, APS regulated the differentiation of Tfh subpopulations in colitis mice, with Tfh10 and Tfr significantly upregulated while Tfh1, Tfh17, and Tfh21 significantly downregulated. In addition, APS significantly upregulated Treg cells and the levels of its associated nuclear transcription factor Foxp3, and cytokine IL-10 in colitis mice. In conclusion, APS effectively alleviated UC by reshaping the balance of Tfh/Treg cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2022.109108DOI Listing
August 2022

Self-assembled HfO-Au nanocomposites with ultra-fine vertically aligned Au nanopillars.

Nanoscale 2022 Aug 4. Epub 2022 Aug 4.

School of Materials Engineering, Purdue University, West Lafayette, 47907, USA.

Oxide-metal-based hybrid materials have gained great research interest in recent years owing to their potential for multifunctionality, property coupling, and tunability. Specifically, oxide-metal hybrid materials in a vertically aligned nanocomposite (VAN) form could produce pronounced anisotropic physical properties, , hyperbolic optical properties. Herein, self-assembled HfO-Au nanocomposites with ultra-fine vertically aligned Au nanopillars (as fine as 3 nm in diameter) embedded in a HfO matrix were fabricated using a one-step self-assembly process. The film crystallinity and pillar uniformity can be obviously improved by adding an ultra-thin TiN-Au buffer layer during the growth. The HfO-Au hybrid VAN films show an obvious plasmonic resonance at 480 nm, which is much lower than the typical plasmonic resonance wavelength of Au nanostructures, and is attributed to the well-aligned ultra-fine Au nanopillars. Coupled with the broad hyperbolic dispersion ranging from 1050 nm to 1800 nm in wavelength, and unique dielectric HfO, this nanoscale hybrid plasmonic metamaterial presents strong potential for the design of future integrated optical and electronic switching devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2nr03104cDOI Listing
August 2022

Synaptotagmin 1-mediated cell membrane penetration and dopamine release enhancement by latroeggtoxin-VI.

Int J Biol Macromol 2022 Jul 30;216:906-915. Epub 2022 Jul 30.

State Key Laboratory of Developmental Biology of Freshwater Fish, Protein Chemistry Laboratory, College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan, China. Electronic address:

Latroeggtoxin-VI (LETX-VI), a proteinaceous neurotoxin mined from the egg transcriptome of spider L. tredecimguttatus, was previously found to promote the release of dopamine from PC12 cells. However, the relevant molecular mechanism has not been fully clear. Here LETX-VI was demonstrated to rapidly penetrate the plasma membrane of PC12 cells via the vesicle exocytosis/endocytosis cycle, during which vesicular transmembrane protein synaptotagmin 1 (Syt1) functions as a receptor, with its vesicle luminal domain interacting with the C-terminal region of LETX-VI. The C-terminal sequence of LETX-VI is the functional region for both entering cells and promoting dopamine release. After gaining entry into the PC12 cells, LETX-VI down-regulated the phosphorylation levels of Syt1 at T201 and T195, thereby facilitating vesicle fusion with plasma membrane and thus promoting dopamine release. The relevant mechanism analysis indicated that LETX-VI has a protein phosphatase 2A (PP2A) activator activity. The present work has not only probed into the Syt1-mediated action mechanism of LETX-VI, but also revealed the structure-function relationship of the toxin, thus suggesting its potential applications in the drug transmembrane delivery and treatment of the diseases related to dopamine release and PP2A activity deficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2022.07.208DOI Listing
July 2022

Hemin-graphene oxide-gold nanoflower-assisted enhanced electrochemiluminescence immunosensor for determination of prostate-specific antigen.

Mikrochim Acta 2022 07 28;189(8):297. Epub 2022 Jul 28.

Department of Blood Transfusion, The Affiliated Hospital of Qingdao University, Qingdao, 266042, People's Republic of China.

An ultrasensitive luminol electrochemiluminescence (ECL) immunosensor was constructed for the detection of prostate specific antigen (PSA) using glucose oxidase-decorated hemin-graphene oxide-gold nanoflowers ternary nanocomposites as probes. Graphene oxide was first modified with hemin and then with gold nanoflowers through an in situ growth method, which has significantly boosted the catalytic activity of this graphene oxide-based peroxidase mimetics. The biocatalytical activity of this ECL immunosensor was thoroughly investigated to achieve selective recognition of the analyte molecules (PSA) by specific binding between antigens and antibodies. The limit of detection was calculated to be 0.32 pg mL with a signal-to-noise ratio of 3. A broad linear range from 7.5 × 10 to 2.5 ng mL was obtained. Such step-by-step assembled biosensor showed controlled nanostructure and exhibited promising application in analysis of human serum samples with a recovery range of 90.6-111.8% and a RSD range of 3.9-5.5%.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-022-05387-2DOI Listing
July 2022

Lithiophilic Sn-Co nano-seeds sealed in a hollow carbon shell to stabilize lithium metal anodes.

Chem Commun (Camb) 2022 Jul 27. Epub 2022 Jul 27.

Hunan Provincial Key Laboratory of Chemical Power Sources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, P. R. China.

A lithiophilic Sn-Co nano-seed sealed in a nitrogen-doped carbon shell is designed to stabilize lithium metal anodes, in which lithiophilic alloys can regulate lithium deposition behavior and the hollow carbon shell is beneficial to prevent agglomeration. The modified lithium anode can be stable for 1350 h and 400 h under 1 mA cm and 5 mA cm in symmetric cells. The [email protected]@Li||LiFePO full cell with a low N/P ratio of 2.12 shows a superior capacity retention of >98% over 250 cycles under 1C.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2cc02932dDOI Listing
July 2022

Pleiotropic Effect of the Gene and Its Combined Effects with Other Loci for Spike and Grain-Related Traits in Wheat.

Plants (Basel) 2022 Jul 13;11(14). Epub 2022 Jul 13.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China.

Club wheat ( ssp. ) with a distinctly compact spike morphology was conditioned by the dominant () locus on chromosome 2D and resulted in a redistribution of spike yield components. The disclosure of the genetic basis of club wheat was a prerequisite for the development of widely adapted, agronomically competitive club wheat cultivars. In this study, we used a recombinant inbred line population derived from a cross between club wheat Hiller and modern cultivar Yangmai 158 to construct a genetic linkage map and identify quantitative trait loci associated with 15 morphological traits. The club allele acted in a semi-dominant manner and the gene was mapped to 370.12-406.29 Mb physical region on the long arm of 2D. Apart from compact spikes, exhibited a pleiotropic effect on ten other agronomic traits, including plant height, three spike-related traits and six grain-related traits. The compact spike phenotype was correlated with decreased grain size and weight, but with an increase in floret fertility and grain number. These pleiotropic effects make club wheat have compatible spike weight with a normal spike from common wheat. The genetic effects of various gene combinations of with four yield-related genes, including , , and , were evaluated. had no epistatic interaction with any of these genes, indicating that their combinations would have an additive effect on other agronomically important traits. Our research provided a theoretical foundation for the potentially effective deployment of gene into modern breeding varieties in combination with other favorable alleles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/plants11141837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316965PMC
July 2022

The comparative effects of sacubitril/valsartan versus enalapril on pulmonary hypertension due to heart failure with a reduced ejection fraction.

Pulm Circ 2022 Jul 1;12(3):e12034. Epub 2022 Jul 1.

Department of Cardiology Handan Central Hospital Handan Hebei China.

The purpose of this study was to investigate the effects of sacubitril/valsartan on right ventricular (RV) function in patients with pulmonary hypertension (PH) due to heart failure with reduced ejection fraction (HFrEF). We prospectively enrolled patients with HFrEF-induced PH admitted to the Department of Cardiology between August 2018 and December 2019. Patients were randomized to receive oral treatment with sacubitril/valsartan or enalapril. Epidemiological data were recorded before treatment. Echocardiography was performed at admission and 6 months of follow-up, and all parameters were compared. Major adverse cardiac events (MACEs) were compared between baseline and 6 months follow-up. There were no significant differences in the baseline characteristics between the two groups. After 6 months of treatment, both treatment groups improved the following parameters from baseline (mean ± SD): left atrium, left ventricle, the left ventricular ejection function (LVEF), RV systolic function (the tricuspid annular plane systolic excursion [TAPSE], the systolic pulmonary artery pressure [sPAP], and TAPSE/sPAP). After 6 months, sacubitril/valsartan improved significantly the following parameters compared with enalapril (all  < 0.05): LVEF (47.07 ± 6.93% vs. 43.47 ± 7.95%); TAPSE (15.33 ± 1.31 vs. 14.78 ± 1.36 mm); sPAP (36.76 ± 14.32 vs. 42.26 ± 12.07 mmHg); and TAPSE/sPAP ratio (0.50 ± 0.23 vs. 0.39 ± 0.14), respectively. There was no difference in readmissions due to recurrent heart failure. Sacubitril/valsartan seems to provide more beneficial effects among patients with HFrEF-induced PH to improve RV function, along with a decrease in pulmonary pressure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/pul2.12034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297686PMC
July 2022

Mutations of Asn321 and Glu322 Improve Resistance of 4-Hydroxyphenylpyruvate Dioxygenase HPPDm to Topramezone.

J Agric Food Chem 2022 Aug 20;70(31):9703-9710. Epub 2022 Jul 20.

Department of Microbiology, Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.

As a highly efficient 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicide, topramezone is an ideal target for herbicide-resistant genetic engineering. In this study, two mutants, K-19 (N321Y) and K-63 (Q166R/E322V), with topramezone resistance increased by 205.3 and 58.5%, respectively, were screened from the random mutation library of HPPDm, a topramezone-resistant HPPD mutant that we previously obtained. Sites N321 and E322 were identified as key sites for increased topramezone resistance by single-site mutation analysis. A mutant KB-145 (N321Y/E322K) was further obtained by saturation mutation at sites N321 and E322. The topramezone resistance of KB-145 increased by 955.3% compared to mutant HPPDm. In conclusion, this study identifies two new sites that significantly affect the topramezone resistance of HPPDm, which provides new insights into the molecular mechanism of herbicide resistance of HPPD, and the acquired mutants have great application potential in the construction of herbicide-resistant crops.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.2c02327DOI Listing
August 2022

Integrative Analysis of Genes Involved in the Global Response to Potato Wart Formation.

Front Plant Sci 2022 29;13:865716. Epub 2022 Jun 29.

Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu, China.

, the causal agent of potato wart disease, poses a major threat to commercial potato production. Understanding the roles of transcriptionally regulated genes following pathogen infection is necessary for understanding the system-level host response to pathogen. Although some understanding of defense mechanisms against infection has been gained for incompatible interactions, the genes and signaling pathways involved in the compatible interaction remain unclear. Based on the collection of wart diseased tubers of a susceptible cultivar, we performed phenotypic and dual RNA-Seq analyses of wart lesions in seven stages of disease progression. We totally detected 5,052 differentially expressed genes (DEGs) by comparing the different stages of infection to uninfected controls. The tendency toward differential gene expression was active rather than suppressed under attack by the pathogen. The number of DEGs step-up along with the development of the disease and the first, third and seventh of the disease stages showed substantially increase of DEGs in comparison of the previous stage. The important functional groups identified Gene ontology (GO) and KEGG enrichment were those responsible for plant-pathogen interaction, fatty acid elongation and phenylpropanoid biosynthesis. Gene coexpression networks, composed of 17 distinct gene modules that contained between 25 and 813 genes, revealed high interconnectivity of the induced response and led to the identification of a number of hub genes enriched at different stages of infection. These results provide a comprehensive perspective on the global response of potato to infection and identify a potential transcriptional regulatory network underlying this susceptible response, which contribute to a better understanding of the potato- pathosystem.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2022.865716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277394PMC
June 2022

Oriented External Electric Fields Regurating the Reaction Mechanism of CH Oxidation Catalyzed by Fe(IV)-Oxo-Corrolazine: Insight from Density Functional Calculations.

Front Chem 2022 29;10:896944. Epub 2022 Jun 29.

School of Chemistry and Chemical Engineering, Guizhou University, Guiyang, China.

Methane is the simplest alkane and can be used as an alternative energy source for oil and coal, but the greenhouse effect caused by its leakage into the air is not negligible, and its conversion into liquid methanol not only facilitates transportation, but also contributes to carbon neutrality. In order to find an efficient method for converting methane to methanol, CH oxidation catalyzed by Fe(IV)-Oxo-corrolazine (Fe(IV)-Oxo-Cz) and its reaction mechanism regulation by oriented external electric fields (OEEFs) are systematically studied by density functional calculations. The calculations show that Fe(IV)-Oxo-Cz can abstract one H atom from CH to form the intermediate with OH group connecting on the corrolazine ring, with the energy barrier of 25.44 kcal mol. And then the product methanol is formed through the following rebound reaction. Moreover, the energy barrier can be reduced to 20.72 kcal mol through a two-state reaction pathway. Furthermore, the effect of OEEFs on the reaction is investigated. We found that OEEFs can effectively regulate the reaction by adjusting the stability of the reactant and the transition state through the interaction of electric field-molecular dipole moment. When the electric field is negative, the energy barrier of the reaction decreases with the increase of electric intensity. Moreover, the OEEF aligned along the intrinsic FeO reaction axis can effectively regulate the ability of forming the OH on the corrolazine ring by adjusting the charges of O and H atoms. When the electric field intensity is -0.010 a.u., the OH can be directly rebounded to the CH· before it is connecting on the corrolazine ring, thus forming the product directly from the transition state without passing through the intermediate with only an energy barrier of 17.34 kcal mol, which greatly improves the selectivity of the reaction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2022.896944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277104PMC
June 2022

Uptake and metabolism of C-triclosan in celery under hydroponic system.

Sci Total Environ 2022 Jul 14;846:157377. Epub 2022 Jul 14.

Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Zhejiang Province, Zhejiang University, Hangzhou 310058, China. Electronic address:

As triclosan is used extensively as an antimicrobial agent, it inevitably enters agroecosystems, when sewage and treated wastewater are applied to agricultural fields. As a result, triclosan can be accumulated into crops and vegetables. Currently, limited information is available on the metabolism of triclosan in vegetables. In this study, the fate of C-triclosan in celery under a hydroponic system was investigated in a 30-day laboratory test. Most (97.7 %) of the C-triclosan accumulated in celery. The bioconcentration factors of triclosan were up to 3140 L kg at day 30. The concentration of C-triclosan in roots (17.8 mg kg) was 57- and 127-fold higher than that in stems (0.31 mg kg) and leaves (0.14 mg kg), respectively, at day 30, suggesting a higher accumulation of triclosan in celery roots and negligible transport to stems and leaves. Moreover, triclosan, as well as its eight metabolites, was detected and identified in celery tissues and the growth medium using C-labelling and LC-Q-TOF-MS analysis methods. Phase I metabolites in the growth medium were from hydroxylation, dechlorination, nitration, and nitrosylation. Phase II metabolism was the major pathway in celery tissues. Monosaccharide, disaccharide, and sulfate conjugates of triclosan were putatively identified. The results represent an important step toward a better evaluation of the behavior of triclosan in vegetables, with notable implications for environmental and human risk assessments of triclosan.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2022.157377DOI Listing
July 2022

Clinical Predictors of Functional Cure in Children 1-6 Years-old with Chronic Hepatitis B.

J Clin Transl Hepatol 2022 Jun 4;10(3):405-411. Epub 2022 Jan 4.

Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, China.

Background And Aims: Hepatitis B surface antigen (HBsAg) clearance is significantly more common in children with chronic hepatitis B (CHB) than in adults; however, the possible influencing factors related to HBsAg loss have yet to be found. This study aimed to explore the efficacy of long-term interferon (IFN)α therapy in treating children with CHB and analyzed the factors influencing functional cure after treatment.

Methods: A total of 236 children aged 1-6 years and diagnosed with CHB via liver biopsy were included in the study, all receiving IFNα treatment (IFNα-2b monotherapy, IFNα-2b followed by lamivudine [LAM] or IFNα-2b combined with LAM) and followed up for 144 weeks. A comprehensive analysis was conducted on clinical data, including biochemical items, serum markers of hepatitis B virus (HBV) and immunological indexes, and logistic regression analysis was used to screen the influencing factors related to HBsAg loss.

Results: The cumulative loss rates of HBsAg were 79.5%, 62.1% and 42.1% at 144 weeks after the start of treatment in the 1-3 years-old group, 3-5 years-old group and 5-7 years-old group, respectively (<0.05). IFNα-2b combined with LAM treatment displayed the highest HBsAg loss rates compared with monotherapy and sequential treatment (=0.011). Younger baseline age and lower HBsAg levels were independent factors for the prediction of HBsAg loss (<0.05). The baseline PreS1 and hepatitis B core antibody levels in the HBsAg loss group were lower than those in the HBsAg non-loss group. In addition, the PreS1 level was positively corelated with the level of HBsAg, HBV DNA and liver inflammation.

Conclusions: Long-term treatment with IFNα was effective in achieving HBsAg loss in CHB children aged 1-6 years-old. Age less than 3 years-old and lower HBsAg levels are independent predictors of functional cure in children with CHB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.14218/JCTH.2021.00142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240240PMC
June 2022

A high-capacity self-sacrificial additive based on electroactive sodiated carbonyl groups for sodium-ion batteries.

Chem Commun (Camb) 2022 Aug 2;58(62):8702-8705. Epub 2022 Aug 2.

Hunan Provincial Key Laboratory of Chemical Power Sources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, P. R. China.

A new type of high-capacity sacrificial additive (NaCO) is proposed to replenish the sodium loss in sodium ion full-cells. The HC//NaV(PO)F full-cells demonstrate significantly enhanced energy density after introducing an appropriate amount of additive.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2cc01812hDOI Listing
August 2022

Effect of aqueous extract of seed of broccoli on inflammatory cytokines and Helicobacter pylori infection: a randomized, double-blind, controlled trial in patients without atrophic gastritis.

Inflammopharmacology 2022 Jul 13. Epub 2022 Jul 13.

Institute of Epidemiology and Statistics, School of Public Health, Lanzhou University, Lanzhou, Gansu Province, China.

The purpose of this study was to investigate the anti-inflammatory effect of an aqueous extract of seed of broccoli (AESB) in Helicobacter pylori (HP)-infected patients without atrophic gastritis. This was a double-centre, randomized, double-blind, controlled study. A total of 110 HP-infected subjects were randomized to receive either AESB or placebo for 2 months. Inflammatory cytokine (IL-8, IFN-γ, TNF-α, CRP, IL-17A, IL-1β, IL-18), pepsinogen I, II (PG I, PG II), and gastrin-17 (G-17) measurements and C-urea breath tests were performed at baseline and at 60 days. At 60 days, there was no significant difference in any of the inflammatory cytokines, pepsinogen or gastrin between the two groups. However, IL-8, IFN-γ, PG I, PG I/PG II ratio (PGR), and G-17 were reduced by 9.02 pg/mL, 5.08 pg/mL, 24.56 ng/mL, 1.75 and 0.3 pmol/L, respectively, in the AESB group compared with baseline (all P < 0.05). The HP eradication rates in the AESB group and placebo group were 11.11 and 3.70% at 60 days, respectively (P > 0.05). No treatment-related adverse events were reported. Thus, AESB may reduce the risk of gastric mucosal lesions and decrease the risk of gastric cancer by relieving inflammatory cytokines. The safety profile of AESB was satisfactory. This study is registered with the Chinese Clinical Trials Registry (Registration No. ChiCTR2100054249).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10787-022-01030-xDOI Listing
July 2022

Characterization and determination of benvitimod, an unknown risk substance in cosmetics, using nuclear magnetic resonance spectroscopy and HPLC-MS/MS.

J Sep Sci 2022 Jul 13. Epub 2022 Jul 13.

National Institutes for Food and Drug Control/NMPA Key Laboratory for Research and Evaluation of Cosmetics, Beijing, 100050, China.

Ultrahigh-performance liquid chromatography-tandem high-resolution mass spectrometry, combined with preparative chromatography and nuclear magnetic resonance spectroscopy, a new method for identifying unknown risk substance structures in cosmetics has been established. Moreover, HPLC-MS/MS was developed for the determination of benvitimod in cosmetics. The sample was collected in ultrahigh-performance liquid chromatography-tandem high-resolution mass spectrometry, and the molecular formula of the unknown was obtained as C H O . After preparative chromatography enrichment and purification, the enriched compound was scanned by nuclear magnetic resonance spectroscopy, and the chemical structure of the unknown compound was confirmed to be benvitimod. Subsequently, the separation was determined in multiple reaction monitoring mode. The results showed that the linearity of benvitimod was good in the range of 1-100 μg/L with a correlation coefficient r  > 0.999; the limits of detection and quantification were 0.02 mg/kg and 0.067 mg/kg; the precision and stability were good; and the average recoveries were 104.2%, 108.2%, and 108.7% for low, medium, and high spiked concentrations, respectively. Forty batches of cosmetics were screened, of which two batches were detected with the illegal addition of benvitimod at 2.48 and 3.13 g/kg. The method effectively solved the loopholes in regulation and provided a research basis for the qualitative identification of structurally unknown compounds in cosmetics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jssc.202200388DOI Listing
July 2022

Construction and characterization of microsatellite markers for the Neptune whelk, Neptunea cumingii.

Mol Biol Rep 2022 Jul 9. Epub 2022 Jul 9.

Department of Marine Organism Taxonomy & Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, P. R. China.

Background: The Neptune whelk, Neptunea cumingii, is an economic gastropod endemic to the East Asia Warm Temperate Biotic Subregion. The study of the development in microsatellite markers maybe beneficial for assessing the genetic diversity and conservation of resources on this dwindling species.

Methods And Results: The microsatellite markers were constructed and characterized through Illumina high-throughput sequencing and capillary electrophoresis techniques. Eleven polymorphic microsatellite loci were screened and validated. The observed heterozygosity and expected heterozygosity of each locus showed a range from 0.0600 to 0.6508 and from 0.7380 to 0.9375, respectively. The average Shannon's information index and polymorphism information content were 2.0828 and 0.8325, respectively. Deviation from all loci was generated from Hardy-Weinberg equilibrium.

Conclusions: The 11 microsatellite markers developed in this study show polymorphic status. These markers are expected to be highly informative for further analysis of genetic diversity in N. cumingii.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-022-07639-zDOI Listing
July 2022

Degradation Law and Service Life Prediction Model of Tunnel Lining Concrete Suffered Combined Effects of Sulfate Attack and Drying-Wetting Cycles.

Materials (Basel) 2022 Jun 23;15(13). Epub 2022 Jun 23.

Key Laboratory of Transportation Tunnel Engineering, Ministry of Education, School of Civil Engineering, Southwest Jiaotong University, Chengdu 610031, China.

The present study explored the degradation law and service life prediction of tunnel lining concrete with different mineral admixtures under coupled actions of sulfate attack (SA) and drying-wetting (DW) cycles. The deterioration resistance coefficient (DRC) of compressive strength and influence coefficients of sulfate concentration, mineral admixture content, water/binder (w/b) ratio, and curing regime on DRC were studied. After that, a new service life prediction model based on damage mechanics was developed and analyzed. Results show that, by increasing the DW cycles, the DRC first increases and then decreases. DRCs of Ordinary Portland cement (OPC), fly ash (FA), and ground granulated blast-furnace slag (GGBS) concrete linearly decrease with the increase of sulfate concentration, while the silica fume (SF) concrete displays a two-stage process; by increasing the admixture content, the DRCs of FA and GGBS concrete exhibit two distinct stages, while the SF concrete depicts a three-stage process; increasing the w/b ratio linearly decreases the DRC; the DRC of curing regime was sequenced as standard curing (SC) > fog curing (FC) > water curing (WC) > same condition curing (SCC). Based on the experimental results, the service life prediction model is applied and validated. The validation results show that the proposed model can accurately predict the lifetime of concrete with different mix proportions. Furthermore, it is found that the mineral admixture can effectively improve the lifetime of concrete, and the composite mineral admixture is more effective than a single mineral admixture in improving the lifetime of concrete.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma15134435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267559PMC
June 2022

Engineering Crystal Orientation of Cathode for Advanced Lithium-ion Batteries: A Minireview.

Chem Rec 2022 Jul 8:e202200128. Epub 2022 Jul 8.

Hunan Provincial Key Laboratory of Chemical Power Sources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, P.R China.

Engineering crystal orientation has attracted widespread attention since it is related to the cyclability and rate performance of cathode materials for lithium-ion batteries (LIBs). Regulating the crystal directional growth with optimal exposed crystal facets is an effective strategy to improve the performance of cathode materials, but still lacks sufficient attention in research field. Herein, we briefly introduce the characterization techniques and identification methods for crystal facets, then summarize and illuminate the major methods for regulating crystal orientation and their internal mechanism. Furthermore, the optimization strategies for layered-, spinel-, and olivine-structure cathodes are discussed based on the characteristic of crystal structure, and the relationship between exposure of special crystal facets and lithium storage performance is deeply analyzed, which could guide the rational design of cathodes for LIBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/tcr.202200128DOI Listing
July 2022

Visible and near-infrared luminescence properties of Nd/Yb co-doped GdO phosphors for highly sensitive optical thermometry.

Dalton Trans 2022 Jul 12;51(27):10612-10622. Epub 2022 Jul 12.

School of Electrical and Computer Engineering, Jilin Jianzhu University, Changchun 130118, China.

Phosphors with rare earth (RE) ions are widely applied to optical temperature measurements. Moreover, the near-infrared (NIR) emission of phosphors has potential applications in the biological field. Herein, GdO:%Nd/Yb(0.2 ≤ ≤ 0.8) samples are successfully synthesized by the sol-gel method, and their phase and morphology are characterized. The XRD data are refined by Rietveld. Under 980 nm continuous wave (CW) laser excitation, the upconversion (UC) and downshift (DS) emissions are studied. The temperature sensing performances of thermally coupled levels (TCLs) and non-thermally coupled levels (non-TCLs) of GdO: Nd/Yb phosphors are investigated through the fluorescence intensity ratio (FIR) technology. In the temperature range of 303-523 K, the maximum relative sensitivity of / reaches 6.54% K, which is based on non-TCLs. At the same time, the maximum relative sensitivity of / reaches 3.13%. In addition, the influence of the laser induced heating (LIH) effect is studied. Compared with square wave (SW) pumping, the relative sensitivity would decrease at CW excitation. Finally, the durability and temperature uncertainty are also discussed. The results prove that the GdO:Nd/Yb samples present higher relative sensitivity and durability and lower temperature uncertainty in the biological window. Therefore, the phosphors can be used as optical thermometers in the biological field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2dt01271eDOI Listing
July 2022

Intrinsic ferroelectricity in Y-doped HfO thin films.

Nat Mater 2022 Aug 27;21(8):903-909. Epub 2022 Jun 27.

Department of Physics and Astronomy, University of Nebraska-Lincoln, Lincoln, NE, USA.

Ferroelectric HfO-based materials hold great potential for the widespread integration of ferroelectricity into modern electronics due to their compatibility with existing Si technology. Earlier work indicated that a nanometre grain size was crucial for the stabilization of the ferroelectric phase. This constraint, associated with a high density of structural defects, obscures an insight into the intrinsic ferroelectricity of HfO-based materials. Here we demonstrate that stable and enhanced polarization can be achieved in epitaxial HfO films with a high degree of structural order (crystallinity). An out-of-plane polarization value of 50 μC cm has been observed at room temperature in Y-doped HfO(111) epitaxial thin films, with an estimated full value of intrinsic polarization of 64 μC cm, which is in close agreement with density functional theory calculations. The crystal structure of films reveals the Pca2 orthorhombic phase with small rhombohedral distortion, underlining the role of the structural constraint in stabilizing the ferroelectric phase. Our results suggest that it could be possible to exploit the intrinsic ferroelectricity of HfO-based materials, optimizing their performance in device applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41563-022-01282-6DOI Listing
August 2022

FISH-based "pan" and "core" karyotypes reveal genetic diversification of Roegneria ciliaris.

J Genet Genomics 2022 Jun 23. Epub 2022 Jun 23.

State Key Laboratory of Crop Genetics and Germplasm Enhancement/Cytogenetics Institute/Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry (CIC-MCP), Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jgg.2022.06.005DOI Listing
June 2022
-->