Publications by authors named "Haiyan Wang"

1,242 Publications

  • Page 1 of 1

Thermodynamic Characteristics of Oxidation and Combustion of Coal under Lean-Oxygen Conditions.

ACS Omega 2021 Jul 1;6(27):17255-17266. Epub 2021 Jul 1.

School of Emergency Management and Safety Engineering, China University of Mining and Technology, Beijing 100083, P. R. China.

Coal spontaneous combustion (CSC) often occurs in environments that are poorly ventilated or under lean-oxygen environments in coal mines or coal seam outcrops. The understanding of the thermodynamic properties of CSC under lean-oxygen conditions is important to avoid safety and environmental problems. In this paper, the mass variation and critical temperature (the minimum temperature required for coal reaction in each stage) of six coals during CSC under lean-oxygen conditions were investigated using thermogravimetric methods. Furthermore, the thermodynamics parameters and kinetic compensation processes during CSC under lean-oxygen conditions were analyzed. The results showed that the oxidation and combustion of coal under lean-oxygen conditions was affected by the coal rank and oxygen concentration. The increase in the critical temperature was more significant when the oxygen concentration was reduced from 10 to 5%. The mechanism functions most likely to cause CSC at different oxygen concentrations were similar. The reaction mechanisms of high-rank coals at a low-temperature oxidation stage were more influenced by oxygen concentration than low-rank coals. Evaluations of kinetic behavior showed that activation energy decreased in a linear manner as the oxygen concentration decreased. The mathematical relationship between the activation energy and the pre-exponential factor indicated that there was a kinetic compensation process during CSC under lean-oxygen conditions. It is also worth noting that the effect of the coal rank on the thermodynamic characteristics of CSC is better than that of oxygen concentration. This work is helpful for enhancing the prediction and prevention of CSC.
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http://dx.doi.org/10.1021/acsomega.1c01096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280641PMC
July 2021

Is Health Contagious?-Based on Empirical Evidence From China Family Panel Studies' Data.

Front Public Health 2021 2;9:691746. Epub 2021 Jul 2.

International Business Research Institute, Zhejiang Gongshang University, Hangzhou, China.

This study empirically analysed the contagion of health using data from China Family Panel Studies. We first controlled variables related to health behaviour, medical conditions, individual characteristics, household characteristics, group characteristics, and prefecture/county characteristics and then employed multiple methods for estimation. The estimates showed that the average health level of others in the community had a significant positive effect on individual self-rated health-health was contagious. The measurement results remained robust after the endogeneity of the core explanatory variables was controlled using two-stage least squares. Furthermore, by analysing the heterogeneity of health contagion, we found that the contagion effect of health varied with the level of medical care, household affiliation, gender, rural/urban areas, and age groups. The contagion effect of health was more pronounced in the elderly population and the rural areas of the central region, where the level of medical care is relatively low, whereas it did not differ significantly between genders. Finally, the learning or imitation mechanism and social interaction mechanism of health contagion were examined.
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http://dx.doi.org/10.3389/fpubh.2021.691746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283520PMC
July 2021

Selective Interface Synthesis of Cobalt Metaphosphate Nanosheet Arrays Motivated by Functionalized Carbon Cloths for Fast and Durable Na/K-Ion Storage.

ACS Appl Mater Interfaces 2021 Jul 19. Epub 2021 Jul 19.

State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute, Sichuan University, Chengdu 610065, China.

Exploiting novel nanomaterials with fast and durable sodium/potassium ion storage capability is key to alleviate the application limitations of lithium-ion batteries. Herein, a novel energy storage material based on cobalt metaphosphate nanosheet arrays self-supported on carbon cloths [Co(PO) NSs/CC] is fabricated by a two-step strategy. This rationally designed strategy avoids the preparation of the complex {Co[OP(OBu)]} precursor, which significantly simplifies the synthesis process. The active CC acts not only as an electrically conductive substrate as usual but also as a functional basis to suppress PH-involved reaction and to promote HPO-involved reaction during the phosphating process, contributing to the formation of Co(PO). The mutual cross-linked porous Co(PO) nanosheets vertically grow on the surface of activated CC, ensuring sufficient electrolyte infiltration and fast electron transport among the electrodes. Sodium ion storage analysis for the Co(PO) NSs/CC electrode reveals a multi-step reaction mechanism with high reversibility, as reflected by the high reversible capacity (667 mA h g at 50 mA g) and excellent cyclability (with almost no capacity decay over 500 cycles). This novel electrode is also well capable of storing potassium ions, exhibiting high reversible capacity, which outperforms most reported anodes for potassium-ion batteries. The development of this novel high-performance nanomaterial would advance the performance of sodium/potassium-ion batteries toward practical applications.
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http://dx.doi.org/10.1021/acsami.1c08950DOI Listing
July 2021

Long-Residence Pneumonia Vaccine Developed Using PEG-Grafted Hybrid Nanovesicles from Cell Membrane Fusion of Mycoplasma and IFN-γ-Primed Macrophages.

Small 2021 Jul 16:e2101183. Epub 2021 Jul 16.

School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.

CD8 T cell responses play a critical regulatory role in protection against mycoplasma infection-related respiratory diseases. Nanovesicles derived from cell membranes have been shown to induce CD8 T cell responses. Moreover, the short residence time of mycoplasma membrane-related vaccines in local lymph nodes limits the efficacy of current mycoplasma vaccines. Here, a long-residence pneumonia vaccine is developed using nanovesicles prepared by cell membrane fusion of Mycoplasma hyopneumoniae and interferon-γ (IFN-γ   )-primed macrophages, which are grafted with polyethylene glycol to increase residence time in the lymph nodes. Upregulation of intercellular adhesion molecule-1 (ICAM-1) on the membrane of IFN-γ-primed macrophages increases the targeting of the hybrid nanovesicle vaccine to the local lymph nodes, with increased CD8 T cell activation. A mechanistic study reveals that CD8 T cell activation is achieved via a pathway involving upregulation of C-C motif chemokine ligand 2/3 expression by E26 transformation-specific sequences, followed by increased immune-stimulatory activity of dendritic cells. In vivo, prophylactic testing reveals that the hybrid nanovesicle vaccine triggers a long-term immune response, as evidenced by a memory CD8 T cell response against mycoplasma infection. The current study provides a new design strategy for mycoplasma vaccines that involves a hybrid method using biological sources and artificial modification.
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http://dx.doi.org/10.1002/smll.202101183DOI Listing
July 2021

Single- and dual-wavelength fiber laser with multi-transverse modes.

Opt Express 2021 Jun;29(13):20299-20306

A single- and dual-wavelength fiber laser with multi-transverse modes is proposed. Mode interference is realized in the core of an optical fiber by writing a long period fiber grating on a few-mode fiber to obtain the LP mode and the LP mode simultaneously. A mode interferometer based on a few-mode long period fiber grating (FM-LPFG) is used as a comb filter in the ring-cavity fiber laser. Single- and dual-wavelength outputs can be achieved in the fiber laser by adjusting a polarization controller (PC). A mode-selective photonic lantern is used to realize mode conversion, and six LP modes, LP, LP, LP, LP, LP, and LP, can be generated. A single- and dual-wavelength fiber laser with multi-transverse modes can be achieved by combining a mode interferometer with a mode-selective photonic lantern. This work has potential applications in mode division multiplexing (MDM) systems to enlarge the capacity of optical communications.
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http://dx.doi.org/10.1364/OE.430258DOI Listing
June 2021

Significant optical force enhancements of nanostructure with balanced gain and loss.

Opt Express 2021 Jul;29(14):22202-22213

In this paper, we theoretically analyze the optical force between a pair of active and passive plasmonic core-shell nanoparticles (NPs). The optical force between the NPs can be either attractive or repulsive near the critical point while the passive dimer provides only attractive force. We reveal that the reversal of attractive or repulsive force is determined by the relative phase of electric dipole (ED) modes, which can be strongly affected by the gain and loss coefficient κ. Compared with the passive dimer with the same size, the active-passive dimer can exhibit a very high repulsive force (about two orders of magnitude) while remaining the same order magnitude attractive force when the value of coefficient is 0.345. Interestingly, we find that the position of the maximum repulsive force occurs near the critical point. We also investigate the influence of variations in geometrical parameters of the dimer and polarization angle on the force. Finally, the numerical results demonstrate that when the dimer is illuminated by a laser beam, the attractive and repulsive forces can also be achieved. The manipulation of optical force can find potential in optical sorting and transport of NPs.
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http://dx.doi.org/10.1364/OE.430719DOI Listing
July 2021

Technical Note: A preliminary study of dual-tracer PET image reconstruction guided by FDG and/or MR kernels.

Med Phys 2021 Jul 12. Epub 2021 Jul 12.

Lauterbur Research Center for Biomedical Imaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Purpose: Clinically, single radiotracer positron emission tomography (PET) imaging is a commonly used examination method; however, since each radioactive tracer reflects the information of only one kind of cell, it easily causes false negatives or false positives in disease diagnosis. Therefore, reasonably combining two or more radiotracers is recommended to improve the accuracy of diagnosis and the sensitivity and specificity of the disease when conditions permit.

Methods: This paper proposes incorporating F-fluorodeoxyglucose (FDG) as a higher-quality PET image to guide the reconstruction of other lower-count C-methionine (MET) PET datasets to compensate for the lower image quality by using a popular kernel algorithm. Specifically, the FDG prior is needed to extract kernel features, and these features were used to build a kernel matrix using a k-nearest-neighbor (kNN) search for MET image reconstruction. We created a 2-D brain phantom to validate the proposed method by simulating sinogram data containing Poisson random noise and quantitatively compared the performance of the proposed FDG-guided kernelized expectation maximization (KEM) method with the performance of Gaussian and non-local means (NLM) smoothed maximum likelihood expectation maximization (MLEM), MR-guided KEM and multi-guided-S KEM algorithms. Mismatch experiments between FDG/MR and MET data were also carried out to investigate the outcomes of possible clinical situations.

Results: In the simulation study, the proposed method outperformed the other algorithms by at least 3.11% in the signal-to-noise ratio (SNR) and 0.68% in the contrast recovery coefficient (CRC), and it reduced the mean absolute error (MAE) by 8.07%. Regarding the tumor in the reconstructed image, the proposed method contained more pathological information. Furthermore, the proposed method was still superior to the MR-guided KEM method in the mismatch experiments.

Conclusions: The proposed FDG-guided KEM algorithm can effectively utilize and compensate for the tissue metabolism information obtained from dual-tracer PET to maximize the advantages of PET imaging.
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http://dx.doi.org/10.1002/mp.15089DOI Listing
July 2021

Potent and protective IGHV3-53/3-66 public antibodies and their shared escape mutant on the spike of SARS-CoV-2.

Nat Commun 2021 07 9;12(1):4210. Epub 2021 Jul 9.

Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong Province, China.

Neutralizing antibodies (nAbs) to SARS-CoV-2 hold powerful potentials for clinical interventions against COVID-19 disease. However, their common genetic and biologic features remain elusive. Here we interrogate a total of 165 antibodies from eight COVID-19 patients, and find that potent nAbs from different patients have disproportionally high representation of IGHV3-53/3-66 usage, and therefore termed as public antibodies. Crystal structural comparison of these antibodies reveals they share similar angle of approach to RBD, overlap in buried surface and binding residues on RBD, and have substantial spatial clash with receptor angiotensin-converting enzyme-2 (ACE2) in binding to RBD. Site-directed mutagenesis confirms these common binding features although some minor differences are found. One representative antibody, P5A-3C8, demonstrates extraordinarily protective efficacy in a golden Syrian hamster model against SARS-CoV-2 infection. However, virus escape analysis identifies a single natural mutation in RBD, namely K417N found in B.1.351 variant from South Africa, abolished the neutralizing activity of these public antibodies. The discovery of public antibodies and shared escape mutation highlight the intricate relationship between antibody response and SARS-CoV-2, and provide critical reference for the development of antibody and vaccine strategies to overcome the antigenic variation of SARS-CoV-2.
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http://dx.doi.org/10.1038/s41467-021-24514-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270942PMC
July 2021

Effects of graphene on morphology, microstructure and transcriptomic profiling of Pinus tabuliformis Carr. roots.

PLoS One 2021 8;16(7):e0253812. Epub 2021 Jul 8.

Shanxi Poplar High-yield Forest Bureau, Datong, Shanxi Province, PR China.

Graphene has shown great potential for improving growth of many plants, but its effect on woody plants remains essentially unstudied. In this work, Pinus tabuliformis Carr. bare-rooted seedlings grown outdoors in pots were irrigated with a graphene solution over a concentration range of 0-50 mg/L for six months. Graphene was found to stimulate root growth, with a maximal effect at 25 mg/L. We then investigated root microstructure and carried out transcript profiling of root materials treated with 0 and 25 mg/L graphene. Graphene treatment resulted in plasma-wall separation and destruction of membrane integrity in root cells. More than 50 thousand of differentially expressed genes (DEGs) were obtained by RNA sequencing, among which 6477 could be annotated using other plant databases. The GO enrichment analysis and KEGG pathway analysis of the annotated DEGs indicated that abiotic stress responses, which resemble salt stress, were induced by graphene treatment in roots, while responses to biotic stimuli were inhibited. Numerous metabolic processes and hormone signal transduction pathways were altered by the treatment. The growth promotion effects of graphene may be mediated by encouraging proline synthesis, and suppression of the expression of the auxin response gene SMALL AUXIN UP-REGULATED RNA 41 (SAUR41), PYL genes which encode ABA receptors, and GSK3 homologs.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253812PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266090PMC
July 2021

mGluR5-Mediated eCB Signaling in the Nucleus Accumbens Controls Vulnerability to Depressive-Like Behaviors and Pain After Chronic Social Defeat Stress.

Mol Neurobiol 2021 Jul 5. Epub 2021 Jul 5.

Department of Anesthesiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, People's Republic of China.

Stress contributes to major depressive disorder (MDD) and chronic pain, which affect a significant portion of the global population, but researchers have not clearly determined how these conditions are initiated or amplified by stress. The chronic social defeat stress (CSDS) model is a mouse model of psychosocial stress that exhibits depressive-like behavior and chronic pain. We hypothesized that metabotropic glutamate receptor 5 (mGluR5) expressed in the nucleus accumbens (NAc) normalizes the depressive-like behaviors and pain following CSDS. Here, we show that CSDS induced both pain and social avoidance and that the level of mGluR5 decreased in susceptible mice. Overexpression of mGluR5 in the NAc shell and core prevented the development of depressive-like behaviors and pain in susceptible mice, respectively. Conversely, depression-like behaviors and pain were exacerbated in mice with mGluR5 knockdown in the NAc shell and core, respectively, compared to control mice subjected to 3 days of social defeat stress. Furthermore, (RS)-2-chloro-5-hydroxyphenylglycine (CHPG), an mGluR5 agonist, reversed the reduction in the level of the endocannabinoid (eCB) 2-arachidonoylglycerol (2-AG) in the NAc of susceptible mice, an effect that was blocked by 3-((2-methyl-1, 3-thiazol-4-yl) ethynyl) pyridine hydrochloride (MTEP), an mGluR5 antagonist. In addition, the injection of CHPG into the NAc shell and core normalized depressive-like behaviors and pain, respectively, and these effects were inhibited by AM251, a cannabinoid type 1 receptor (CB1R) antagonist. Based on these results, mGluR5-mediated eCB production in the NAc relieves stress-induced depressive-like behaviors and pain.
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http://dx.doi.org/10.1007/s12035-021-02469-9DOI Listing
July 2021

Sevoflurane postconditioning reduces hypoxia/reoxygenation injury in cardiomyocytes via up-regulating heat shock proteins 70.

J Microbiol Biotechnol 2021 Jun 11;31(8). Epub 2021 Jun 11.

Department of Anesthesiology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, P. R. China.

Sevoflurane postconditioning (SPostC) is proved effective against myocardial cardioprotection injury. It has been reported that heat shock protein 70 (HSP70) could be induced by sevoflurane, which played a crucial role in hypoxic/reoxygenation (HR) injury of cardiomyocytes. The mechanism of sevoflurane to protect cardiomyocytes via HSP70 is still understood. We aimed to investigate the related mechanisms of SPostC inducing HSP70 expression to reduce the HR injury of cardiomyocytes. After HR cardiomyocytes model was established, the cells transfected with siRNA for HSP70 (siHSP70) or not were treated with sevoflurane during reoxygenation. The lactate dehydrogenase (LDH) level was detected by colorimetry, cell viability and apoptosis were detected by MTT and flow cytometry. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were used to detect HSP70, apoptosis-, cell cycle-associated factors, iNOS, and Cox-2 expressions. Enzyme-linked immuno sorbent assay (ELISA) was used to measure malondialdehyde (MDA) and superoxide dismutase (SOD). SPostC decreased apoptosis, cell injury, oxidative stress, and inflammation and increased viability of HR-induced cardiomyocytes. Besides, SPostC down-regulated Bax and Cleaved caspase-3 levels, while SPostC up-regulated Bcl-2, CDK-4, CyclinD1, and HSP70 levels. SiHSP70 had the opposite effect which SPostC had on HR-induced cardiomyocytes. Moreover, siHSP70 further reversed the effect of SPostC on apoptosis, cell injury, oxidative stress, inflammation, viability and the expressions of HSP70, apoptosis-, and cell cycle-associated factors in HR-induced cardiomyocytes. In conclusion, this study demonstrated that SPostC reduced the HR injury of cardiomyocytes by inducing HSP70 expression.
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http://dx.doi.org/10.4014/jmb.2103.03040DOI Listing
June 2021

Magnetron sputtering of strontium nanolayer on zirconia implant to enhance osteogenesis.

Mater Sci Eng C Mater Biol Appl 2021 Aug 19;127:112191. Epub 2021 May 19.

Department of Oral and Maxillofacial Surgery/Pathology, Amsterdam UMC and Academic Center for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam (VU), Amsterdam Movement Science (AMS), Amsterdam, the Netherlands; Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU), Amsterdam, the Netherlands.

The zirconia implants have a wide range of clinical applications, however, the biological inertness and lack of osteoinductive properties limit these applications. Strontium possesses superior biocompatibility and excellent osteogenic properties. To take advantage of these, the strontium titanate-coated zirconia implants were prepared in this study by sandblasting, acid etching, and magnetron sputtering, followed by the analysis of the biological behavior. Briefly, the zirconia sheets were polished and subjected to sandblasting and acid etching. Subsequently, a nano‑strontium titanate coating was developed on the sheets by magnetron sputtering. The specimens were characterized by scanning electron microscopy (SEM), water contact angle measurement (WCA) and EDS mapping, which confirmed the physical alternation and successful deposition of the strontium titanate coating. The in vitro experiments indicated that the majority of the filopodia and actin fibers of the MC3T3-E1 cells on SA-ZrO/Sr possessed an optimal osteogenic property to promote the osteogenic differentiation. Moreover, the RT-PCR results revealed that SA-ZrO/Sr significantly up-regulated the gene expression of Runx2, COL-1, ALP, OPG, OPN and OCN. Further, the in vivo evaluation confirmed that the SA-ZrO/Sr implants promoted the bone-implant osseointegration to the greatest extent as compared to SA-ZrO and ZrO implant. Overall, the SA-ZrO/Sr system was confirmed to be a promising implant, thus, providing new pathways for an effective implant design.
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http://dx.doi.org/10.1016/j.msec.2021.112191DOI Listing
August 2021

High-strength nanocrystalline intermetallics with room temperature deformability enabled by nanometer thick grain boundaries.

Sci Adv 2021 Jul 2;7(27). Epub 2021 Jul 2.

School of Materials Engineering, Purdue University, West Lafayette, IN 47907, USA.

Although intermetallics are attractive for their high strength, many of them are often brittle at room temperature, thereby severely limiting their potential as structural materials. Here, we report on a previously unidentified deformable nanocrystalline CoAl intermetallics with Co-rich thick grain boundaries (GBs). In situ micropillar compression studies show that nanocrystalline CoAl with thick GBs exhibits ultrahigh yield strength, exceeding 4.5 gigapascals. Unexpectedly, nanocrystalline CoAl intermetallics also show prominent work hardening to a flow stress of 5.7 gigapascals up to 20% compressive strain. Transmission electron microscopy studies show that deformation induces abundant dislocations inside CoAl grains with thick GBs, which accommodate plastic deformation. Molecular dynamics simulations reveal that the Co-rich thick GBs play a vital role in promoting nucleation of dislocations at the Co/CoAl interfaces, thereby enhancing the plasticity of the intermetallics. This study provides a perspective to promoting the plasticity of intermetallics via the introduction of thick GBs.
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http://dx.doi.org/10.1126/sciadv.abc8288DOI Listing
July 2021

A general scattering proximity immunoassay with the formation of dimer of gold nanoparticle.

Talanta 2021 Oct 26;233:122515. Epub 2021 May 26.

Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong, 266071, PR China.

In this work, we structured a colorimetric ultrasensitive detection of carcinoembryonic antigen (CEA) based on a proximity hybridization-induced gold nanoparticles (Au NPs) dimers structure. Under the dark-field microscope, this method takes advantage of the distinctive and strong distance-relative localized surface plasmon resonance (LSPR) of Au NPs and their oriented assembly. DNA served as a medium showing wonderful flexibility to label antibody and Au NPs, and tune interparticle spacing as well. Two capture probes were formed by the integration of DNA labeled antibody (DNA1-Ab1 or DNA2-Ab2) and asymmetrically assembled DNA (DNA 3 or DNA 4)- Au NPs via partly hybridization between DNA sequences. In the presence of antigen, the reaction between target protein and capture probes could trigger the generation of immunocomplex which led to the proximity hybridization of the DNA1 and DNA2, and then change the distance of interparticle to form Au NP dimers and thus showed a different color under dark-field microscope. A limit of detection of 14.25 pg/mL was obtained for the detection of CEA, which indicated a promising sensing method in clinical diagnosis of protein biomarkers.
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http://dx.doi.org/10.1016/j.talanta.2021.122515DOI Listing
October 2021

Transcriptome-Wide Analysis of RNA mA Methylation and Gene Expression Changes Among Two Ecotypes and Their Reciprocal Hybrids.

Front Plant Sci 2021 10;12:685189. Epub 2021 Jun 10.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Key Laboratory for Information Agriculture, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, China.

The remodeling of transcriptome, epigenome, proteome, and metabolome in hybrids plays an important role in heterosis. N(6)-methyladenosine (mA) methylation is the most abundant type of post-transcriptional modification for mRNAs, but the pattern of inheritance from parents to hybrids and potential impact on heterosis are largely unknown. We constructed transcriptome-wide mRNA mA methylation maps of Col-0 and (Ler) and their reciprocal F hybrids. Generally, the transcriptome-wide pattern of mA methylation tends to be conserved between accessions. Approximately 74% of mA methylation peaks are consistent between the parents and hybrids, indicating that a majority of the mA methylation is maintained after hybridization. We found a significant association between differential expression and differential mA modification, and between non-additive expression and non-additive methylation on the same gene. The overall RNA mA level between Col-0 and Ler is clearly different but tended to disappear at the allelic sites in the hybrids. Interestingly, many enriched biological functions of genes with differential mA modification between parents and hybrids are also conserved, including many heterosis-related genes involved in biosynthetic processes of starch. Collectively, our study revealed the overall pattern of inheritance of mRNA mA modifications from parents to hybrids and a potential new layer of regulatory mechanisms related to heterosis formation.
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http://dx.doi.org/10.3389/fpls.2021.685189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222996PMC
June 2021

Remediation of heavy metal-polluted alkaline vegetable soil using mercapto-grafted palygorskite: effects of field-scale application and soil environmental quality.

Environ Sci Pollut Res Int 2021 Jun 22. Epub 2021 Jun 22.

Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, 300191, China.

Remediation materials are the most critical factors for in situ immobilization of soil contaminated by heavy metals. In this study, in order to improve the performance of palygorskite (Pal), a new remediation material, mercapto-grafted palygorskite (MPal) was synthesized by grafting mercapto groups onto the surface of Pal. The results of field application in northern China showed that at a dosage of 0.12-0.23 kg m, MPal significantly reduced the available concentrations of Cd, Pb, and Cr in the soil by 52.2%, 29.9%, and 46.2%, respectively. Concurrently, Cd, Pb, and Cr concentrations in the shoots of head lettuce also decreased significantly, with the highest reduction being 44.0%, 61.5%, and 50.0%, respectively. At the same dosage, MPal had a better immobilization effect than Pal. There was no significant change in the pH of the vegetable soil, while the zeta potential decreased significantly, indicating that the MPal did not immobilize the heavy metals by increasing the pH, making it suitable for alkaline farmland soil. In addition, soil environmental quality was improved overall. MPal increased the activities of urease, β-glucosidase, cellulase, and catalase by 15.4%, 56.5%, 7.8%, and 14.9%, respectively. It increased the number of fungi and actinomycetes by 4.5% and 23.1%, respectively. MPal, as a new remediation material for soil contaminated by heavy metals, could achieve efficient remediation effects when applied in small doses. Compared with Pal, it is environmentally friendly, is low cost, and is more suitable for the treatment of heavy metal pollution in large areas of farmland.
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http://dx.doi.org/10.1007/s11356-021-15034-7DOI Listing
June 2021

Microbubbles intensification and mechanism of wet air oxidation process of MDEA-containing wastewater.

Environ Technol 2021 Jun 28:1-12. Epub 2021 Jun 28.

College of Chemical Engineering, Nanjing Tech University, Nanjing, People's Republic of China.

In order to the intensification of gas-liquid mass transfer of MDEA-containing wastewater during wet air oxidation (WAO) process, the microbubbles and millimetre bubbles were applied by fine-pore sparger (5 and 20-30 μm) and single pore sparger (6.35 mm), respectively. Effect of the superficial gas velocity on the average microbubble size, gas holdup and oxygen mass transfer coefficient (Ka) of MDEA-containing wastewater at the ambient conditions was studied. The results showed that the microbubbles (less than 1 mm) were beneficial to enhance mass transfer process and had a higher dissolved oxygen concentration during WAO process of MDEA-containing wastewater owing to higher gas holdup and larger oxygen mass transfer coefficient. The COD removal ratio was 66% at low superficial gas velocity (u= 0.3 cm/s) in WAO process by microbubbles, while it achieved at high superficial gas velocity (u= 3.0 cm/s) by millimetre bubbles. The critical oxygen mass transfer coefficient Ka was 0.183 min of MDEA-containing wastewater by 20-30 and 5 μm fine pore sparger, which was 2∼5 times more than that of single pore sparger (<0.1 min). The microbubbles could improve dissolved oxygen concentration and enhance the formation of hydroxyl radical at short time with atmospheric pressure. During the WAO process, the MDEA would be converted into intermediates including formic acid, acetic acid, ammonium, nitrite and nitrate. The WAO process with microbubbles could significantly improve the gas-liquid mass transfer performance at low superficial gas velocity and greatly reduce air consumption for MDEA-containing wastewater.
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http://dx.doi.org/10.1080/09593330.2021.1945687DOI Listing
June 2021

The Use of Aerosolized Medications in Adult Intensive Care Unit Patients: A Prospective, Multicenter, Observational, Cohort Study.

J Aerosol Med Pulm Drug Deliv 2021 Jun 14. Epub 2021 Jun 14.

Department of Respiratory and Critical Care Medicine, West China Medical Center, Sichuan University, Chengdu, China.

Only limited data are available on the real-life clinical utilization of aerosolized medications in intensive care unit (ICU) patients. Exploring the utilization of aerosolized medications in the ICU may contribute to develop appropriate education and improve the quality of aerosol therapy. A 2-week, prospective, multicenter, observational, cohort study was conducted to record how the aerosolized medications were utilized in the Chinese ICUs, including indications, medications used in solo or combination, dosage, and side-effects in adult patients. A total of 1006 patients from 28 ICUs were enrolled, of which 389 (38.7%) received aerosol therapy. The most common indications for aerosol therapy were difficulty in secretion management (23.1%) and chronic obstructive pulmonary disease exacerbation (18.5%). The combination of inhaled corticosteroids and short-acting muscarinic antagonist was the most commonly used medication (19.5%, 76/389). Ninety-two percent (358/389) of the patients did not have any side effects during aerosol therapy. More patients in the group with mechanical ventilation received bronchodilators than spontaneous breathing patients (81.3% vs. 55.5%,  < 0.001), and more patients who breathed spontaneously through a tracheostomy received mucus-regulating agents than other patients (70% vs. 37.9%,  = 0.004). In mainland China, more than one-third of adult ICU patients received aerosol therapy. Medications utilized during aerosol therapy were variable in patients with different respiratory support. To promote appropriate use of aerosolized medications, high-quality randomized, controlled trials and clinical guidance on aerosolized medication indications and dosing are needed to improve clinical outcomes.
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http://dx.doi.org/10.1089/jamp.2021.0004DOI Listing
June 2021

Qualitative and quantitative determination of trace aldehydes and ketones in food preservative propionic acid for quality improvement.

Anal Methods 2021 Jul;13(26):2989-2996

College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, China.

Aiming at the difficulty in qualitative and quantitative analysis of trace compositions in food preservative propionic acid, the trace compositions and the key components influencing the total aldehyde content in propionic acid were analyzed by gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC) and gas chromatography (GC) in this paper. The results show that the food preservative propionic acid contains 18 trace compositions. The main compositions affecting the total aldehyde content in propionic acid are acetaldehyde, propionaldehyde, 1-cyclopropyl-1-propanone and 2-methyl-2-pentenal, which account for ∼90% of the total aldehyde content in propionic acid. The total aldehyde content in propionic acid can be quickly determined by iodometric titration. However, the impurity compositions, solution oxygen content and pH value of propionic acid may affect the stability of NaHSO3, which lead to a higher titration value of the aldehyde content. The total aldehyde contents in propionic acid determined by GC (148.535-337.890 mg L-1) and HPLC (157.730-335.780 mg L-1) are close and reliable with a relative deviation of 0.31-3.00%. The volatile low-carbon aldehydes and ketones including formaldehyde, acetaldehyde and acetone have a weak response and a high detection limit in GC. The derivation conditions of all aldehydes and ketones in propionic acid should affect the determination results of HPLC. It provides the basic data for quality improvement and ultra-deep purification of food-grade propionic acid.
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http://dx.doi.org/10.1039/d1ay00742dDOI Listing
July 2021

Identification of Differentially Expressed Plasma lncRNAs As Potential Biomarkers for Breast Cancer.

Clin Breast Cancer 2021 May 18. Epub 2021 May 18.

Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, China. Electronic address:

Background: Breast cancer is the most common malignant tumor in women and is not easy to diagnose. Increasing evidence has underscored that long non-coding RNAs (lncRNAs) play important regulatory roles in the occurrence and progression of many cancers, including breast cancer. We aimed to identify lncRNAs in plasma as potential biomarkers for breast cancer.

Patients And Methods: We analyzed the Gene Expression Omnibus (GEO) datasets GSE22820, GSE42568, and GSE65194 to identify the common differential genes between cancer tissues and adjacent tissues. Then 14 lncRNAs were identified among the common differential genes and validated by using real-time quantitative polymerase chain reaction in 92 patients with breast cancer and 100 healthy controls. Receiver operating characteristic (ROC) curves were constructed to evaluate their diagnostic value for breast cancer.

Results: Integrated analysis of the GEO datasets identified three significantly upregulated and 11 downregulated lncRNAs in breast cancer tissues. Compared with healthy controls, MIAT was significantly upregulated in breast cancer patient plasma, and LINC00968 and LINC01140 were significantly downregulated. ROC curve analysis suggested that these three lncRNAs can discriminate breast cancer from healthy individual with high specificity and sensitivity.

Conclusion: This research identified three differentially expressed lncRNAs in breast cancer patient plasma. Our data suggest that these three lncRNAs can be used as potential diagnostic biomarkers of breast cancer.
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http://dx.doi.org/10.1016/j.clbc.2021.05.003DOI Listing
May 2021

Evaluation of the prognostic value of CBXs in gastric cancer patients.

Sci Rep 2021 Jun 11;11(1):12375. Epub 2021 Jun 11.

Department of Epidemiology, College of Public Health, Hainan Medical University, Longhua District, No.3 Xueyuan Road, Haikou, 570216, China.

Chromobox (CBX) proteins were suggested to exert epigenetic regulatory and transcriptionally repressing effects on target genes and might play key roles in the carcinogenesis of a variety of carcinomas. Nevertheless, the functions and prognostic significance of CBXs in gastric cancer (GC) remain unclear. The current study investigated the roles of CBXs in the prognosis of GC using the Oncomine, The Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN, The Cancer Genome Atlas (TCGA), and cBioPortal databases. CBX1/2/3/4/5 were significantly upregulated in GC tissues compared with normal tissues, and CBX7 was downregulated. Multivariate analysis showed that high mRNA expression levels of CBX3/8 were independent prognostic factors for prolonged OS in GC patients. In addition, the genetic mutation rate of CBXs was 37% in GC patients, and genetic alterations in CBXs showed no association with OS or disease-free survival (DFS) in GC patients. These results indicated that CBX3/8 can be prognostic biomarkers for the survival of GC patients.
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http://dx.doi.org/10.1038/s41598-021-91649-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196000PMC
June 2021

Analysis of sagittal curvature and its influencing factors in adolescent idiopathic scoliosis.

Medicine (Baltimore) 2021 Jun;100(23):e26274

Department of Anatomy, Inner Mongolia Medical University.

Abstract: This study aimed to explore the characteristics of changes in the sagittal arrangement of the spine between adolescent patients with idiopathic scoliosis (AIS) and normal adolescents, the risk factors for AIS and the factors affecting the progress of AIS.X-ray images of the full length of the spine in standing position were taken in AIS patients and normal adolescents. Radiographic measurements made at intermediate follow-up included the following:C1 and C2 cervical lordosis and C2 - C7 curvature of cervical lordosis, C2-C7sagittal horizontal distance (C2-C7SagittalVerticalAxis, C2-C7SVA), TS-CL, after thoracic lobe (Thoracic Kyphosis, TK), thoracic lumbar segment Angle (thoracolumbar kyphosis, [TLK]), lumbar lordosis Angle (Lumbar Lordosis, LL), sacral slope Angle (Sacrum Slope, SS), pelvic tilt Angle (Pelvic Tilt, PT), pelvic incidence (PI), L5 Incidence (Lumbar5 Slope (L5S), L5 incidence (Lumbar5 Incidence (L5I), sagittal horizontal distance (CSVA), lower depression Angle of the 2nd cervical spine. The difference of sagittal plane parameters between AIS group and normal adolescent group was compared. To evaluate the progress of AIS, correlation analysis was conducted between diagonal 2 and other parameters. The main risk factors of AIS were determined by binary Logistic analysis.The CSVA of AIS patients was higher than that of healthy adolescents (AIS: 27.64 ± 19.56) mm. Healthy adolescents: (17.74 ± 12.8) mm), L5S (AIS: 19.93°= 7.07° and healthy adolescents: 15.38°= 7.78°, P = .024 < .05), C2 downward sag Angle (AIS: 15.12°= 2.7°;Healthy adolescents: 12.97°= 4.56°); AIS patients had lower TS-CL (AIS: 22.48 ± 6.09 and healthy adolescents: 28.26°= 10.32°), PT (AIS: 10.42°= 4.53° and healthy adolescents: 15.80°=7.68°), (AIS: 41.87°=9.72° and healthy adolescents: 48.75°= 8.22°). The main risk factor for idiopathic scoliosis in adolescents was L5 (OR = 1.239, 95%CI = 1.049-1.463, P = .012 < .05).L5S is a major risk factor for idiopathic scoliosis in adolescents. The larger PI is, the higher the risk of scoliosis progression is. In AIS patients, lumbar lordosis is increased, cervical lordosis is reduced, and even cervical kyphosis occurs.
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http://dx.doi.org/10.1097/MD.0000000000026274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202640PMC
June 2021

Vertical and seasonal changes in soil carbon pools to vegetation degradation in a wet meadow on the Qinghai-Tibet Plateau.

Sci Rep 2021 Jun 10;11(1):12268. Epub 2021 Jun 10.

College of Forestry, Gansu Agricultural University, Lanzhou, 730070, China.

Wet meadows provide opportunities to decrease carbon dioxide (CO) and methane (CH) released into the atmosphere by increasing the soil organic carbon (SOC) stored in wetland systems. Although wet meadows serve as the most important and stable C sinks, there has been very few investigations on the seasonal distributions of SOC fractions in high-altitude wet meadows. Here, we studied the effects of four vegetation degradation levels, non-degraded (ND), lightly degraded (LD), moderately degraded (MD), and heavily degraded (HD), on the measured vertical and seasonal changes of SOC and its different fractions. Among these vegetation degradation levels, 0-10 and 10-20 cm soil depths in ND plots had significantly higher SOC contents than the other degradation levels had throughout the year. This is attributed to the relatively greater inputs of aboveground plant litter and richer fine-root biomass in ND plots. Particulate organic carbon (POC) and light fraction organic carbon (LFOC) showed similar vertical and seasonal variations in autumn, reaching a minimum. Moreover, microbial biomass (MBC) and easily oxidizable organic carbon (EOC) contents were highest in summer and the smallest in winter, while dissolved organic carbon (DOC) content was highest in spring and lowest in summer, and were mainly concentrated in the 0-20 cm layer. Pearson correlation analysis indicated that soil properties and aboveground biomass were significantly related to different SOC fractions. The results indicate that vegetation degradation reduces the accumulation of total SOC and its different fractions, which may reduce carbon sink capacity and soil quality of alpine wet meadows, and increase atmospheric environmental pressure. In addition, vegetation biomass and soil characteristics play a key role in the formation and transformation of soil carbon. These results strengthen our understanding of soil C dynamics, specifically related to the different C fractions as affected by vegetation degradation levels and soil depth, in wet meadow systems.
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http://dx.doi.org/10.1038/s41598-021-90543-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192520PMC
June 2021

Intratumoral expression of interleukin 23 variants using oncolytic vaccinia virus elicit potent antitumor effects on multiple tumor models via tumor microenvironment modulation.

Theranostics 2021 3;11(14):6668-6681. Epub 2021 May 3.

Allegheny Health Network Cancer Institute, Pittsburgh, PA 15212, USA.

Newly emerging cancer immunotherapy has led to significant progress in cancer treatment; however, its efficacy is limited in solid tumors since the majority of them are "cold" tumors. Oncolytic viruses, especially when properly armed, can directly target tumor cells and indirectly modulate the tumor microenvironment (TME), resulting in "hot" tumors. These viruses can be applied as a cancer immunotherapy approach either alone or in combination with other cancer immunotherapies. Cytokines are good candidates to arm oncolytic viruses. IL-23, an IL-12 cytokine family member, plays many roles in cancer immunity. Here, we used oncolytic vaccinia viruses to deliver IL-23 variants into the tumor bed and explored their activity in cancer treatment on multiple tumor models. Oncolytic vaccinia viruses expressing IL-23 variants were generated by homologue recombination. The characteristics of these viruses were evaluated by RT-qPCR, ELISA, flow cytometry and cytotoxicity assay. The antitumor effects of these viruses were evaluated on multiple tumor models and the mechanisms were investigated by RT-qPCR and flow cytometry. IL-23 prolonged viral persistence, probably mediated by up-regulated IL-10. The sustainable IL-23 expression and viral oncolysis elevated the expression of Th1 chemokines and antitumor factors such as IFN-γ, TNF-α, Perforin, IL-2, Granzyme B and activated T cells in the TME, transforming the TME to be more conducive to antitumor immunity. This leads to a systemic antitumor effect which is dependent on CD8 and CD4 T cells and IFN-γ. Oncolytic vaccinia viruses could not deliver stable IL-23A to the tumor, attributed to the elevated tristetraprolin which can destabilize the IL-23A mRNA after the viral treatment; whereas vaccinia viruses could deliver membrane-bound IL-23 to elicit a potent antitumor effect which might avoid the possible toxicity normally associated with systemic cytokine exposure. Either secreted or membrane-bound IL-23-armed vaccinia virus can induce potent antitumor effects and IL-23 is a candidate cytokine to arm oncolytic viruses for cancer immunotherapy.
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http://dx.doi.org/10.7150/thno.56494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171085PMC
May 2021

Enhanced electrochemiluminescence ratiometric cytosensing based on surface plasmon resonance of Au nanoparticles and nanosucculent films.

Biosens Bioelectron 2021 Oct 1;189:113367. Epub 2021 Jun 1.

Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong, 266071, PR China.

Ramos cells are human Burkitt's lymphoma cells, which are a kind of cancer cells to facilitate the monitoring of the relevant biological processes of cancers. Sensitive and accurate detection of Ramos cells using emerging ratiometric ECL biosensing technology shows increasing importance, however, the target analytes of current ratiometric ECL biosensors are mainly limited to DNA/RNA or proteins. In this study, we proposed a dual-potential ratiometric sensing strategy for the electrochemiluminescence detection of Ramos cells based on two types of electrochemiluminescence (ECL)-responding molecular. Au nanosucculent films (AuNFs) were electrodeposited on the fluorine doped tin oxide (FTO) electrode to increase the effective area of the electrode for more efficient assembly of DNA and effectively improving the conductivity of the sensing interfaces. In the presence of Ramos cells, aptamers capped with [email protected] would conjugate with Ramos cells and then remove from AuNFs, accompanying the decrease of ECL signal from [email protected] Then, Au-DNA was captured and alternately hybridized with DNA-modified CdS nanocrystals (NCs) on the surface of AuNFs with the formation of a super reticulate structure. The reticulate structure not only raised another identified ECL signal from CdS NCs but also greatly promoted its ECL intensity from the surface plasmon resonance originating from Au NPs. The value of log (ECL/ECL) and the logarithm of the number of cells exhibit considerable linear relation ranging from 80 to 8 × 10 cells mL with a low detection limit of 20 cells mL (S/N = 3). The selectivity and specificity of this dual-potential ECL sensor showed good performance and indicated considerable promise in avoiding false-positive results in detection.
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http://dx.doi.org/10.1016/j.bios.2021.113367DOI Listing
October 2021

A Case of Malignant Atrophic Papulosis With Multiple Complications.

Am J Gastroenterol 2021 May 26. Epub 2021 May 26.

Department of Radiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, PRC; Key Laboratory of the Ministry of Education for Experimental Teratology, Department of Histology and Embryology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, PRC.

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http://dx.doi.org/10.14309/ajg.0000000000001322DOI Listing
May 2021

Intrauterine insemination (IUI) with or without letrozole for unexplained or mild male factor infertility: A randomized pilot study.

Eur J Obstet Gynecol Reprod Biol 2021 Jul 15;262:216-220. Epub 2021 May 15.

Department of Obstetrics and Gynaecology, School of Clinical Sciences at Monash Health, Monash University, Clayton, Australia.

Objective: To study the feasibility of a randomized controlled trial (RCT) comparing intrauterine insemination (IUI) with and without letrozole in couples with unexplained or mild male factor infertility STUDY DESIGN: We performed a randomized pilot study including 100 couples with unexplained or mild male factor infertility in the Reproductive Medicine Centre of Peking University Third Hospital in China. The couples scheduled for IUI were randomized to IUI with or without ovarian stimulation (letrozole) for up to 3 cycles within a time horizon of 4 months. Women in the letrozole group received 5 mg oral letrozole daily starting from cycle day 3-5 for 5 days. Women in the natural cycle IUI group did not receive any ovarian stimulation before IUI. The primary outcome is ongoing pregnancy leading to live birth. The study was registered under trial number NCT03455426 RESULTS: Between March 2018 and January 2019, 158 couples were eligible to participate after initial screening and 100 (63.3 %) couples agreed to participate. Of the 100 recruited couples, 50 were randomly allocated to IUI with letrozole and 50 to natural cycle IUI. Live birth occurred in 12 women (24.0 %) in the letrozole group and 10 women (20.0 %) in the natural cycle group (RR 1.20 (95 % CI 0.57-2.52)). Clinical pregnancy rates were 28 % and 26 % in the letrozole group and natural cycle group respectively (RR 1.08 (95 % CI 0.56-2.05). There were no multiple pregnancies in both groups. Patients were willing to be randomized and useful information was gained to plan a definitive trial.

Conclusions: We showed that an RCT comparing IUI with letrozole versus natural cycle IUI in couples with unexplained or mild male factor infertility is feasible and acceptable.
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http://dx.doi.org/10.1016/j.ejogrb.2021.05.029DOI Listing
July 2021

Metabolic Defects of Peripheral T Cells in COVID-19 Patients.

J Immunol 2021 May 28. Epub 2021 May 28.

Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital; The Second Affiliated Hospital, School of Medicine, South University of Science and Technology, Shenzhen, Guangdong Province, China;

The relatively low partial pressure of oxygen, reduced oxygen saturation, and aberrant plasma metabolites in COVID-19 may alter energy metabolism in peripheral immune cells. However, little is known regarding the immunometabolic defects of T cells in COVID-19 patients, which may contribute to the deregulated immune functions of these cells. In this study, we longitudinally characterized the metabolic profiles of resting and activated T cells from acutely infected and convalescent COVID-19 patients by flow cytometry and confirmed the metabolic profiles with a Seahorse analyzer. Non-COVID-19 and healthy subjects were enrolled as controls. We found that ex vivo T cells from acutely infected COVID-19 patients were highly activated and apoptotic and displayed more extensive mitochondrial metabolic dysfunction, especially cells in CD8 T cell lineages, than those from convalescent COVID-19 patients or healthy controls, but slightly disturbed mitochondrial metabolic activity was observed in non-COVID-19 patients. Importantly, plasma IL-6 and C-reactive protein (CRP) levels positively correlated with mitochondrial mass and negatively correlated with fatty acid uptake in T cells from COVID-19 patients. Additionally, compared with those from healthy controls, in vitro-activated T cells from acutely infected COVID-19 patients showed signs of lower glycolysis, a reduced glycolytic capacity, and a decreased glycolytic reserve, accompanied by lower activation of mTOR signaling. Thus, newly identified defects in T cell mitochondrial metabolic functions and metabolic reprogramming upon activation might contribute to immune deficiency in COVID-19.
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http://dx.doi.org/10.4049/jimmunol.2100068DOI Listing
May 2021

A natural variation of an SVP MADS-box transcription factor in Triticum petropavlovskyi leads to its ectopic expression and contributes to elongated glume.

Mol Plant 2021 May 25. Epub 2021 May 25.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, Jiangsu 210095, China. Electronic address:

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http://dx.doi.org/10.1016/j.molp.2021.05.022DOI Listing
May 2021

Biochemical and cytotoxic evaluation of latroeggtoxin-VI against PC12 cells.

J Biochem Mol Toxicol 2021 May 28:e22825. Epub 2021 May 28.

State Key Laboratory of Developmental Biology of Freshwater Fish, Protein Chemistry Laboratory, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.

Latroeggtoxin-VI (LETX-VI) is a peptide neurotoxin discovered from Latrodectus tredecimguttatus eggs. In the current study, the action features of the neurotoxin on PC12 cells were systematically investigated. LETX-VI could promote dopamine release from PC12 cells in the absence and presence of Ca2+, involving an even more complex action mechanism in the presence of Ca2+ and when the treatment time was longer. Although LETX-VI enchanced the autophagy and secretion activity in PC 12 cells, it showed no remarkable influence on the proliferation, cell cycle, apoptosis and ultrastructure of the cells. Pulldown combined with CapLC-MS/MS analysis suggested that LETX-VI affected PC12 cells by interacting with multiple proteins involved in the metabolism, transport, and release of neurotransmitters, particularly dopamine. The low cytotoxicity and effective regulatory action of LETX-VI on PC12 cells suggest the potential of the active peptide in the development of drugs for the treatment of some dopamine-related psychotic diseases and cancers.
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http://dx.doi.org/10.1002/jbt.22825DOI Listing
May 2021